Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0013140

Physical Location & Seq

Genomic Coordinates Reverse (-)

40374029 .. 40376945

Gene Structure 2917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1322300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGGCCAAATCGTCGGCGACATCGACGCCGTCATTTCCGCCGCTCAAGCCCGACGACTACTCCCACAGCCCGGTTCACTACGCCGTCGTTCTGGCTGACCACACCACGCTCTCCCGAATCGTCGCCACCCTTCCGCGACTCGCCGATCCTACTCAGATCCACACCGAGTCCGACTCCCTCTCTCAGGAACGGACCGCGGACCGGATCTCATCCGTACTCGACCGCCGCGACGTTCCCTTCCGAGAAACCCCTCTCCACCTCGCCGTCCGGCTAAACGACGCAGCGTCCGCCAAAATTCTAGCCTCCGCAGGCGCTGACGTGTCCCTCCAGAACTCCGCCGGCTGGAATCCCCTGCAGGAAGCCTTATGTCGCCGGAGCTCCGACGTTGCTTTAATCCTTCTCCGGCACCACCACCGCTCCGCTTGGGCGAAGTGGCGGCGCCGTCTTCCACGTGTCATCGCCGTGCTCCGAAGAATGCGAGATTTTTACATGGAGATCTCTTTCCACTTCGAAAGCTCCGTCATTCCCTTCGTCGGGAAGATCGCTCCCTCCGACACGTACAAGATCTGGAAGCGCGACGGCAACTTGCGCGCTGACACGTCGCTGGCCGGATTTGACGGATTGAAGATCCAGCGCGCCGATCAGAGCTTCCTCTTCCTCGGCGACGGCGATCAAAGCCACGACGTTCCCCCGGGATCGCTGCTCGTTTTGAACCGCGACGATCGGAAAATCTTCGACGCGTTCGAGAACGCCGGGTCGCCGATGAGTGAGTCGGACATCGCCGGATTCTGCTCCCAGACGAGTGTGTACCGCCCCGGCATGGACGTCACCAAGGCGGAGCTCGTCGGGCGGACCAATTGGAGGAGGCAGGATAAGACGGAGAGCGTCGGAGAGTGGAAAGCTCGAGTGTACGAAATTCACAATGTCGTGTTCATCTTCCGCTCGAGAAAAGTCGCCAATGGCGACGCTGATGTGGCGGGGAGCGAGCAGGTGCTTCCCCTCGAGCTCGACGAGGACGACGACGGTTTTTTGGTGGCGGAGAATCCGAGCTTCGGAATTCCCGACGGCAGGAGGCACAGTAGCTTCGTGAGGGAGGATAGGGAGTTCGTGGCATTGGGGAGAAAAAGTGTCGACGTTTCGTCAATTTCCGGACCACCGTCAAGGGTGGCATTGGGGAGAAAAAGCGTGGACGTTTCGTCAATTTCCGGGCCACCATCAAGGGTGGCATTGGGGAGAAAAAGCGTCGACGTTTCGTCAATTTCCGGGCCTCCGTCGAGGAGGTCTAAAACAGCTGCGATTGCTGCGGCGCCGGTGCAGACGAAAGAGAAAGAGTTCGTGAGGAGCTTGAGGCCGTGCGTTTGGCTGACGGAGCAGTTCCCGTTAAAGACGGAGGAGCTGCTGCCGTTACTGGACATCTTGGCGAACAAGGTGAAGGCCGTTAGGAGGATGAGGGAGTTGCTCACCACCAAGTTCCCGCCAGGGACTTTTCCGGTTAAGGTGGCAATACCGGTGGTCCCCACAGTGAGAGTGGTGATAACATTCACGAAGTTCGTTGAGCTCCCGCCAACTGAGCAATTCTTCACTCCAATGTCAAGCCCCAGACAGTTTATTCATGAAGGACGAGGCCAGCAAGAGGAGGAACACAAATACAAGGCGCAAAATCCACCATCCTTTTCGTCATCGTCGTGGCTGAGGCGGAACAGCAGCCAGTCGGGGTCTTCCGCGAGCAAGCAAAACCACCACCAACACCAACACTGTGCTCCTCCGGCAGTGGCGGCGGCTCAAGACTCTTCTGACCCTTTCGCCATTCCTAGTGGTTATACGTGGACGAGCATCGAGGACAAGTCTAGCAAAATGAAGAAATCCAAGTCCACGAGGAGATCGTCCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006621 GO:0008104 GO:0008150 GO:0009889 GO:0009987 GO:0010556 GO:0010869 GO:0010941 GO:0012505 GO:0019222 GO:0031323 GO:0032507 GO:0033036 GO:0033365 GO:0034613 GO:0035437 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0048471 GO:0050789 GO:0050794 GO:0051179 GO:0051235 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0070972 GO:0072595 GO:2000209

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 GPCR_chapero_1

Protein Analysis

631

Amino Acids

69.95

Weight (kDa)

9.58

Isoelectric Point (pI)

61.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	78 - 136	3.3e-06	Ankyrin repeats (3 copies)
ANKRD13_C	PF11904	197 - 608	4.6e-102	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62050
fragaria_vesca	FvH4_3g01950
malus_domestica	MD05G1347900.v1.1 MD10G1322300.v1.1
prunus_persica	Prupe.4G019600_v2.0.a1
pyrus_communis	pycom05g31540 pycom10g27280
rosa_chinensis	RchiOBHm_Chr5g0002861
rosa_laevigata	RLG00000031066
rosa_multiflora	Rmu_sc0013603.1_g000009 Rmu_sc0020722.1_g000002
rosa_roxburghii	Rroxscaffold_1G00072990
rosa_rugosa	Rorug04G0400300
rosa_samantha	Rh5AG023200 Rh5BG025900 Rh5CG025800 Rh5DG024300
rosa_wichuraiana	Rw5G002160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	979
AatII	GACGTC	1 cut(s)	828
Acc36I	ACCTGC	1 cut(s)	979
AccB1I	GGYRCC	3 cut(s)	405, 438, 1306
AccBSI	CCGCTC	3 cut(s)	43, 417, 942
AccI	GTMKAC	2 cut(s)	1131, 1245
AccII	CGCG	9 cut(s)	136, 197, 228, 576, 591, 636, 717, 740, 1724
AccIII	TCCGGA	1 cut(s)	1148
AclWI	GGATC	5 cut(s)	140, 151, 212, 622, 703
AcoI	YGGCCR	2 cut(s)	3, 606
AcsI	RAATTY	3 cut(s)	294, 915, 1056
AcvI	CACGTG	1 cut(s)	452
AcyI	GRCGYC	4 cut(s)	26, 439, 825, 1307
AfaI	GTAC	4 cut(s)	216, 560, 809, 911
AflIII	ACRYGT	5 cut(s)	318, 451, 555, 597, 738
AgeI	ACCGGT	1 cut(s)	1507
AgsI	TTSAA	2 cut(s)	625, 712
AhdI	GACNNNNNGTC	4 cut(s)	283, 1138, 1195, 1252
AjiI	CACGTC	2 cut(s)	319, 600
AjnI	CCWGG	1 cut(s)	1477
AloI	GAACNNNNNNTCC	2 cut(s)	1088, 1120
Alw21I	GWGCWC	6 cut(s)	380, 468, 843, 1008, 1560, 1762
AlwI	GGATC	5 cut(s)	140, 151, 212, 622, 703
AlwNI	CAGNNNCTG	1 cut(s)	314
Ama87I	CYCGRG	4 cut(s)	691, 903, 943, 1001
Aor13HI	TCCGGA	1 cut(s)	1148
AoxI	GGCC	7 cut(s)	3, 606, 1208, 1265, 1349, 1434, 1624
ApeKI	GCWGC	7 cut(s)	281, 700, 1292, 1301, 1396, 1399, 1704
ApoI	RAATTY	3 cut(s)	294, 915, 1056
ArsI	GACNNNNNNTTYG	2 cut(s)	504, 536
AsiGI	ACCGGT	1 cut(s)	1507
Asp700I	GAANNNNTTC	2 cut(s)	731, 1331
AspLEI	GCGC	9 cut(s)	314, 441, 576, 591, 593, 636, 638, 1309, 1657
AspS9I	GGNCC	7 cut(s)	192, 199, 852, 1151, 1208, 1265, 1513
AsuC2I	CCSGG	7 cut(s)	71, 692, 693, 754, 816, 1207, 1264
AsuHPI	GGTGA	4 cut(s)	820, 1441, 1453, 1543
AsuII	TTCGAA	1 cut(s)	510
AvaI	CYCGRG	4 cut(s)	691, 903, 943, 1001
AvaII	GGWCC	5 cut(s)	192, 199, 852, 1151, 1513
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	405, 438, 1306
BanII	GRGCYC	4 cut(s)	380, 843, 1008, 1560
BauI	CACGAG	1 cut(s)	1873
BbrPI	CACGTG	1 cut(s)	452
BbsI	GAAGAC	2 cut(s)	437, 1710
Bbv12I	GWGCWC	6 cut(s)	380, 468, 843, 1008, 1560, 1762
BbvCI	CCTCAGC	1 cut(s)	1691
BbvI	GCAGC	7 cut(s)	293, 687, 1279, 1288, 1383, 1386, 1716
BccI	CCATC	2 cut(s)	1222, 1675
BciT130I	CCWGG	1 cut(s)	1479
BcnI	CCSGG	7 cut(s)	71, 692, 693, 754, 816, 1207, 1264
BfaI	CTAG	3 cut(s)	299, 1812, 1848
BfmI	CTRYAG	1 cut(s)	353
BfoI	RGCGCY	3 cut(s)	315, 442, 1310
BfuAI	ACCTGC	1 cut(s)	979
BglII	AGATCT	2 cut(s)	495, 564
Bme1390I	CCNGG	8 cut(s)	71, 692, 693, 754, 816, 1207, 1264, 1479
Bme18I	GGWCC	5 cut(s)	192, 199, 852, 1151, 1513
BmeRI	GACNNNNNGTC	4 cut(s)	283, 1138, 1195, 1252
BmeT110I	CYCGRG	4 cut(s)	691, 903, 943, 1001
BmgBI	CACGTC	2 cut(s)	319, 600
BmgT120I	GGNCC	7 cut(s)	192, 199, 852, 1151, 1208, 1265, 1513
BmiI	GGNNCC	4 cut(s)	407, 440, 1308, 1515
BmrFI	CCNGG	8 cut(s)	71, 692, 693, 754, 816, 1207, 1264, 1479
BmsI	GCATC	1 cut(s)	1842
BoxI	GACNNNNGTC	1 cut(s)	1156
BpiI	GAAGAC	2 cut(s)	437, 1710
BplI	GAGNNNNNCTC	2 cut(s)	158, 190
BpmI	CTGGAG	1 cut(s)	311
Bpu10I	CCTNAGC	1 cut(s)	1691
Bpu14I	TTCGAA	1 cut(s)	510
BpuEI	CTTGAG	3 cut(s)	29, 1366, 1767
BpuMI	CCSGG	7 cut(s)	71, 692, 693, 754, 816, 1207, 1264
BsaAI	YACGTR	3 cut(s)	452, 558, 1824
BsaHI	GRCGYC	4 cut(s)	26, 439, 825, 1307
BsaJI	CCNNGG	7 cut(s)	195, 658, 690, 691, 814, 831, 1478
BsaWI	WCCGGW	4 cut(s)	201, 1148, 1489, 1507
BsaXI	ACNNNNNCTCC	8 cut(s)	44, 74, 401, 431, 1063, 1093, 1111, 1141
Bse118I	RCCGGY	3 cut(s)	338, 1309, 1507
Bse1I	ACTGG	2 cut(s)	1413, 1708
BseAI	TCCGGA	1 cut(s)	1148
BseBI	CCWGG	1 cut(s)	1479
BseDI	CCNNGG	7 cut(s)	195, 658, 690, 691, 814, 831, 1478
BseGI	GGATG	3 cut(s)	209, 1452, 1667
BseMII	CTCAG	4 cut(s)	167, 197, 1560, 1682
BseNI	ACTGG	2 cut(s)	1413, 1708
BsePI	GCGCGC	2 cut(s)	589, 634
BseRI	GAGGAG	7 cut(s)	877, 1291, 1354, 1406, 1649, 1752, 1891
BseXI	GCAGC	7 cut(s)	293, 687, 1279, 1288, 1383, 1386, 1716
BsgI	GTGCAG	1 cut(s)	1334
Bsh1236I	CGCG	9 cut(s)	136, 197, 228, 576, 591, 636, 717, 740, 1724
Bsh1285I	CGRYCG	2 cut(s)	223, 724
BshFI	GGCC	7 cut(s)	5, 608, 1210, 1267, 1351, 1436, 1626
BshNI	GGYRCC	3 cut(s)	405, 438, 1306
BshTI	ACCGGT	1 cut(s)	1507
BsiEI	CGRYCG	2 cut(s)	223, 724
BsiHKAI	GWGCWC	6 cut(s)	380, 468, 843, 1008, 1560, 1762
BsiHKCI	CYCGRG	4 cut(s)	691, 903, 943, 1001
BslFI	GGGAC	3 cut(s)	307, 1495, 1499
BsmFI	GGGAC	3 cut(s)	307, 1495, 1499
BsmI	GAATGC	1 cut(s)	480
BsnI	GGCC	7 cut(s)	5, 608, 1210, 1267, 1351, 1436, 1626
BsoBI	CYCGRG	4 cut(s)	691, 903, 943, 1001
Bsp119I	TTCGAA	1 cut(s)	510
Bsp1286I	GDGCHC	6 cut(s)	380, 468, 843, 1008, 1560, 1762
Bsp13I	TCCGGA	1 cut(s)	1148
BspANI	GGCC	7 cut(s)	5, 608, 1210, 1267, 1351, 1436, 1626
BspCNI	CTCAG	4 cut(s)	166, 196, 1561, 1683
BspEI	TCCGGA	1 cut(s)	1148
BspFNI	CGCG	9 cut(s)	136, 197, 228, 576, 591, 636, 717, 740, 1724
BspHI	TCATGA	1 cut(s)	1612
BspLI	GGNNCC	4 cut(s)	407, 440, 1308, 1515
BspMAI	CTGCAG	1 cut(s)	357
BspMI	ACCTGC	1 cut(s)	979
BspPI	GGATC	5 cut(s)	140, 151, 212, 622, 703
BspT104I	TTCGAA	1 cut(s)	510
BspT107I	GGYRCC	3 cut(s)	405, 438, 1306
BsrBI	CCGCTC	3 cut(s)	43, 417, 942
BsrFI	RCCGGY	3 cut(s)	338, 1309, 1507
BsrI	ACTGG	2 cut(s)	1413, 1708
BssAI	RCCGGY	3 cut(s)	338, 1309, 1507
BssECI	CCNNGG	7 cut(s)	195, 658, 690, 691, 814, 831, 1478
BssHII	GCGCGC	2 cut(s)	589, 634
BssNI	GRCGYC	4 cut(s)	26, 439, 825, 1307
BssSI	CACGAG	1 cut(s)	1873
BssT1I	CCWWGG	1 cut(s)	831
Bst2BI	CACGAG	1 cut(s)	1873
Bst2UI	CCWGG	1 cut(s)	1479
Bst4CI	ACNGT	6 cut(s)	1025, 1079, 1158, 1522, 1605, 1757
Bst6I	CTCTTC	2 cut(s)	659, 1795
BstACI	GRCGYC	4 cut(s)	26, 439, 825, 1307
BstBAI	YACGTR	3 cut(s)	452, 558, 1824
BstBI	TTCGAA	1 cut(s)	510
BstC8I	GCNNGC	9 cut(s)	310, 340, 591, 606, 636, 986, 1628, 1726, 1730
BstDEI	CTNAG	4 cut(s)	153, 183, 1569, 1691
BstDSI	CCRYGG	1 cut(s)	195
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	3 cut(s)	209, 1452, 1667
BstFNI	CGCG	9 cut(s)	136, 197, 228, 576, 591, 636, 717, 740, 1724
BstH2I	RGCGCY	3 cut(s)	315, 442, 1310
BstHHI	GCGC	9 cut(s)	314, 441, 576, 591, 593, 636, 638, 1309, 1657
BstMCI	CGRYCG	2 cut(s)	223, 724
BstNI	CCWGG	1 cut(s)	1479
BstPAI	GACNNNNGTC	1 cut(s)	1156
BstSCI	CCNGG	8 cut(s)	69, 690, 691, 752, 814, 1205, 1262, 1477
BstSFI	CTRYAG	1 cut(s)	353
BstUI	CGCG	9 cut(s)	136, 197, 228, 576, 591, 636, 717, 740, 1724
BstV1I	GCAGC	7 cut(s)	293, 687, 1279, 1288, 1383, 1386, 1716
BstV2I	GAAGAC	2 cut(s)	437, 1710
BstX2I	RGATCY	5 cut(s)	156, 204, 495, 564, 627
BstYI	RGATCY	5 cut(s)	156, 204, 495, 564, 627
BsuRI	GGCC	7 cut(s)	5, 608, 1210, 1267, 1351, 1436, 1626
BtgI	CCRYGG	1 cut(s)	195
BtgZI	GCGATG	2 cut(s)	442, 763
BtrI	CACGTC	2 cut(s)	319, 600
BtsCI	GGATG	3 cut(s)	209, 1452, 1667
BtsI	GCAGTG	1 cut(s)	1776
BtsIMutI	CAGTG	3 cut(s)	1527, 1753, 1776
BveI	ACCTGC	1 cut(s)	979
Cac8I	GCNNGC	9 cut(s)	310, 340, 591, 606, 636, 986, 1628, 1726, 1730
CaiI	CAGNNNCTG	1 cut(s)	314
CciI	TCATGA	1 cut(s)	1612
CfoI	GCGC	9 cut(s)	314, 441, 576, 591, 593, 636, 638, 1309, 1657
Cfr10I	RCCGGY	3 cut(s)	338, 1309, 1507
Cfr13I	GGNCC	7 cut(s)	192, 199, 852, 1151, 1208, 1265, 1513
Cfr42I	CCGCGG	1 cut(s)	198
Cfr9I	CCCGGG	1 cut(s)	691
CpoI	CGGWCCG	2 cut(s)	192, 199
CseI	GACGC	7 cut(s)	34, 273, 287, 746, 874, 974, 1231
Csp6I	GTAC	4 cut(s)	215, 559, 808, 910
CspAI	ACCGGT	1 cut(s)	1507
CspCI	CAANNNNNGTGG	2 cut(s)	1491, 1526
CspI	CGGWCCG	2 cut(s)	192, 199
CviAII	CATG	3 cut(s)	490, 820, 1613
CviQI	GTAC	4 cut(s)	215, 559, 808, 910
DdeI	CTNAG	4 cut(s)	153, 183, 1569, 1691
DinI	GGCGCC	2 cut(s)	440, 1308
DriI	GACNNNNNGTC	4 cut(s)	283, 1138, 1195, 1252
EaeI	YGGCCR	2 cut(s)	3, 606
Eam1104I	CTCTTC	2 cut(s)	659, 1795
Eam1105I	GACNNNNNGTC	4 cut(s)	283, 1138, 1195, 1252
EarI	CTCTTC	2 cut(s)	659, 1795
EciI	GGCGGA	7 cut(s)	27, 277, 325, 851, 865, 1052, 1711
Ecl136II	GAGCTC	4 cut(s)	378, 841, 1006, 1558
Eco130I	CCWWGG	1 cut(s)	831
Eco24I	GRGCYC	4 cut(s)	380, 843, 1008, 1560
Eco47I	GGWCC	5 cut(s)	192, 199, 852, 1151, 1513
Eco53kI	GAGCTC	4 cut(s)	378, 841, 1006, 1558
Eco72I	CACGTG	1 cut(s)	452
Eco88I	CYCGRG	4 cut(s)	691, 903, 943, 1001
EcoICRI	GAGCTC	4 cut(s)	378, 841, 1006, 1558
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoRI	GAATTC	1 cut(s)	1056
EcoRII	CCWGG	1 cut(s)	1477
EcoT14I	CCWWGG	1 cut(s)	831
EcoT38I	GRGCYC	4 cut(s)	380, 843, 1008, 1560
EgeI	GGCGCC	2 cut(s)	440, 1308
EheI	GGCGCC	2 cut(s)	440, 1308
ErhI	CCWWGG	1 cut(s)	831
FaeI	CATG	3 cut(s)	493, 823, 1616
FaiI	YATR	5 cut(s)	367, 491, 821, 1614, 1821
FaqI	GGGAC	3 cut(s)	307, 1495, 1499
FatI	CATG	3 cut(s)	489, 819, 1612
FauI	CCCGC	3 cut(s)	970, 1482, 1569
FblI	GTMKAC	2 cut(s)	1131, 1245
FokI	GGATG	3 cut(s)	196, 1459, 1654
FriOI	GRGCYC	4 cut(s)	380, 843, 1008, 1560
FspBI	CTAG	3 cut(s)	299, 1812, 1848
GlaI	GCGC	9 cut(s)	313, 440, 575, 590, 592, 635, 637, 1308, 1656
GsuI	CTGGAG	1 cut(s)	311
HaeII	RGCGCY	3 cut(s)	315, 442, 1310
HaeIII	GGCC	7 cut(s)	5, 608, 1210, 1267, 1351, 1436, 1626
HgaI	GACGC	7 cut(s)	34, 273, 287, 746, 874, 974, 1231
HhaI	GCGC	9 cut(s)	314, 441, 576, 591, 593, 636, 638, 1309, 1657
Hin1I	GRCGYC	4 cut(s)	26, 439, 825, 1307
Hin1II	CATG	3 cut(s)	493, 823, 1616
Hin6I	GCGC	9 cut(s)	312, 439, 574, 589, 591, 634, 636, 1307, 1655
HinP1I	GCGC	9 cut(s)	312, 439, 574, 589, 591, 634, 636, 1307, 1655
HincII	GTYRAC	2 cut(s)	1132, 1246
HindII	GTYRAC	2 cut(s)	1132, 1246
HinfI	GANTC	9 cut(s)	117, 138, 167, 173, 346, 770, 786, 1042, 1787
HphI	GGTGA	4 cut(s)	820, 1441, 1453, 1543
Hpy166II	GTNNAC	8 cut(s)	76, 808, 910, 1132, 1189, 1246, 1827, 1872
Hpy8I	GTNNAC	8 cut(s)	76, 808, 910, 1132, 1189, 1246, 1827, 1872
HpyAV	CCTTC	6 cut(s)	140, 247, 407, 538, 1426, 1610
HpyCH4III	ACNGT	6 cut(s)	1025, 1079, 1158, 1522, 1605, 1757
HpyCH4V	TGCA	2 cut(s)	355, 1315
HpyF3I	CTNAG	4 cut(s)	153, 183, 1569, 1691
Hsp92I	GRCGYC	4 cut(s)	26, 439, 825, 1307
Hsp92II	CATG	3 cut(s)	493, 823, 1616
HspAI	GCGC	9 cut(s)	312, 439, 574, 589, 591, 634, 636, 1307, 1655
KasI	GGCGCC	2 cut(s)	438, 1306
Kpn2I	TCCGGA	1 cut(s)	1148
KroI	GCCGGC	1 cut(s)	338
KroNI	GCCGGC	1 cut(s)	340
KspI	CCGCGG	1 cut(s)	198
Lsp1109I	GCAGC	7 cut(s)	293, 687, 1279, 1288, 1383, 1386, 1716
LweI	GCATC	1 cut(s)	1842
MaeI	CTAG	3 cut(s)	299, 1812, 1848
MaeIII	GTNAC	2 cut(s)	826, 1404
MbiI	CCGCTC	3 cut(s)	43, 417, 942
MfeI	CAATTG	1 cut(s)	856
MflI	RGATCY	5 cut(s)	156, 204, 495, 564, 627
MhlI	GDGCHC	6 cut(s)	380, 468, 843, 1008, 1560, 1762
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	294, 856, 915, 1056, 1143, 1200, 1257, 1574
MluI	ACGCGT	1 cut(s)	738
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	439, 1307
MlyI	GAGTC	5 cut(s)	132, 167, 176, 779, 1781
MmeI	TCCRAC	5 cut(s)	195, 405, 576, 753, 868
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	1 cut(s)	1148
MroNI	GCCGGC	1 cut(s)	338
MroXI	GAANNNNTTC	2 cut(s)	731, 1331
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	392, 1382, 1494
MslI	CAYNNNNRTG	3 cut(s)	461, 1586, 1684
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	197, 1292
MspR9I	CCNGG	8 cut(s)	71, 692, 693, 754, 816, 1207, 1264, 1479
MunI	CAATTG	1 cut(s)	856
Mva1269I	GAATGC	1 cut(s)	480
MvaI	CCWGG	1 cut(s)	1479
MvnI	CGCG	9 cut(s)	136, 197, 228, 576, 591, 636, 717, 740, 1724
NaeI	GCCGGC	1 cut(s)	340
NarI	GGCGCC	2 cut(s)	439, 1307
NciI	CCSGG	7 cut(s)	71, 692, 693, 754, 816, 1207, 1264
NgoMIV	GCCGGC	1 cut(s)	338
NlaIII	CATG	3 cut(s)	493, 823, 1616
NlaIV	GGNNCC	4 cut(s)	407, 440, 1308, 1515
NmeAIII	GCCGAG	1 cut(s)	639
NmuCI	GTSAC	1 cut(s)	826
NspV	TTCGAA	1 cut(s)	510
PaeR7I	CTCGAG	3 cut(s)	903, 943, 1001
PagI	TCATGA	1 cut(s)	1612
PaqCI	CACCTGC	1 cut(s)	979
PauI	GCGCGC	2 cut(s)	589, 634
PctI	GAATGC	1 cut(s)	480
PdiI	GCCGGC	1 cut(s)	340
PdmI	GAANNNNTTC	2 cut(s)	731, 1331
PfeI	GAWTC	4 cut(s)	117, 346, 786, 1042
PflFI	GACNNNGTC	1 cut(s)	28
PinAI	ACCGGT	1 cut(s)	1507
Ple19I	CGATCG	1 cut(s)	724
PleI	GAGTC	5 cut(s)	132, 167, 175, 778, 1781
PluTI	GGCGCC	2 cut(s)	442, 1310
PmaCI	CACGTG	1 cut(s)	452
PmlI	CACGTG	1 cut(s)	452
PpsI	GAGTC	5 cut(s)	132, 167, 175, 778, 1781
Ppu21I	YACGTR	3 cut(s)	452, 558, 1824
PshAI	GACNNNNGTC	1 cut(s)	1156
Psp124BI	GAGCTC	4 cut(s)	380, 843, 1008, 1560
Psp6I	CCWGG	1 cut(s)	1477
PspCI	CACGTG	1 cut(s)	452
PspGI	CCWGG	1 cut(s)	1477
PspN4I	GGNNCC	4 cut(s)	407, 440, 1308, 1515
PspPI	GGNCC	7 cut(s)	192, 199, 852, 1151, 1208, 1265, 1513
PspXI	VCTCGAGB	2 cut(s)	903, 1001
PstI	CTGCAG	1 cut(s)	357
PstNI	CAGNNNCTG	1 cut(s)	314
PsuI	RGATCY	5 cut(s)	156, 204, 495, 564, 627
PsyI	GACNNNGTC	1 cut(s)	28
PteI	GCGCGC	2 cut(s)	589, 634
PvuI	CGATCG	1 cut(s)	724
PvuII	CAGCTG	1 cut(s)	1292
RsaI	GTAC	4 cut(s)	216, 560, 809, 911
RsaNI	GTAC	4 cut(s)	215, 559, 808, 910
RseI	CAYNNNNRTG	3 cut(s)	461, 1586, 1684
Rsr2I	CGGWCCG	2 cut(s)	192, 199
RsrII	CGGWCCG	2 cut(s)	192, 199
SacI	GAGCTC	4 cut(s)	380, 843, 1008, 1560
SacII	CCGCGG	1 cut(s)	198
SalI	GTCGAC	2 cut(s)	1130, 1244
SaqAI	TTAA	3 cut(s)	392, 1382, 1494
Sau96I	GGNCC	7 cut(s)	192, 199, 852, 1151, 1208, 1265, 1513
SbfI	CCTGCAGG	1 cut(s)	357
SchI	GAGTC	5 cut(s)	132, 167, 176, 779, 1781
ScrFI	CCNGG	8 cut(s)	71, 692, 693, 754, 816, 1207, 1264, 1479
SdaI	CCTGCAGG	1 cut(s)	357
SduI	GDGCHC	6 cut(s)	380, 468, 843, 1008, 1560, 1762
SfaNI	GCATC	1 cut(s)	1842
SfcI	CTRYAG	1 cut(s)	353
SfoI	GGCGCC	2 cut(s)	440, 1308
Sfr274I	CTCGAG	3 cut(s)	903, 943, 1001
Sfr303I	CCGCGG	1 cut(s)	198
SfuI	TTCGAA	1 cut(s)	510
SgrAI	CRCCGGYG	1 cut(s)	1309
SgrBI	CCGCGG	1 cut(s)	198
SgrDI	CGTCGACG	1 cut(s)	1244
SinI	GGWCC	5 cut(s)	192, 199, 852, 1151, 1513
SlaI	CTCGAG	3 cut(s)	903, 943, 1001
SmaI	CCCGGG	1 cut(s)	693
SmiMI	CAYNNNNRTG	3 cut(s)	461, 1586, 1684
SmlI	CTYRAG	6 cut(s)	44, 903, 943, 1001, 1345, 1782
SmoI	CTYRAG	6 cut(s)	44, 903, 943, 1001, 1345, 1782
Sse8387I	CCTGCAGG	1 cut(s)	357
Sse9I	AATT	8 cut(s)	294, 856, 915, 1056, 1143, 1200, 1257, 1574
SspDI	GGCGCC	2 cut(s)	438, 1306
SspMI	CTAG	3 cut(s)	299, 1812, 1848
SstI	GAGCTC	4 cut(s)	380, 843, 1008, 1560
StyD4I	CCNGG	8 cut(s)	69, 690, 691, 752, 814, 1205, 1262, 1477
StyI	CCWWGG	1 cut(s)	831
TaaI	ACNGT	6 cut(s)	1025, 1079, 1158, 1522, 1605, 1757
TasI	AATT	8 cut(s)	294, 856, 915, 1056, 1143, 1200, 1257, 1574
TauI	GCSGC	6 cut(s)	43, 228, 439, 1307, 1778, 1781
TfiI	GAWTC	4 cut(s)	117, 346, 786, 1042
Tru1I	TTAA	3 cut(s)	392, 1382, 1494
Tru9I	TTAA	3 cut(s)	392, 1382, 1494
TscAI	CASTG	3 cut(s)	1527, 1760, 1776
TseFI	GTSAC	1 cut(s)	826
TseI	GCWGC	7 cut(s)	281, 700, 1292, 1301, 1396, 1399, 1704
Tsp45I	GTSAC	1 cut(s)	826
TspDTI	ATGAA	4 cut(s)	922, 1601, 1629, 1871
TspGWI	ACGGA	8 cut(s)	202, 205, 508, 633, 893, 1260, 1382, 1403
TspMI	CCCGGG	1 cut(s)	691
TspRI	CASTG	3 cut(s)	1527, 1760, 1776
Tth111I	GACNNNGTC	1 cut(s)	28
VpaK11BI	GGWCC	5 cut(s)	192, 199, 852, 1151, 1513
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	3 cut(s)	294, 915, 1056
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1525
XhoI	CTCGAG	3 cut(s)	903, 943, 1001
XmaI	CCCGGG	1 cut(s)	691
XmiI	GTMKAC	2 cut(s)	1131, 1245
XmnI	GAANNNNTTC	2 cut(s)	731, 1331
XspI	CTAG	3 cut(s)	299, 1812, 1848
ZraI	GACGTC	1 cut(s)	826

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.