Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0013140

Physical Location & Seq

Genomic Coordinates Forward (+)

1822890 .. 1825567

Gene Structure 2678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G002160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGCAAAGTCGTCGTCGTCGTCCTCCTCGACGGCGTCGTTTCCGCCTCTCAAGCCCGACGACTACTCCCACAGCCGCGTCCACTACACCGTCGTTTTGGCAGACCACGCCACTCTCTCCCGAATCGTCTCCACTCCCCCGCCTCGCCGATCCGGCTCAGATCCACACCGAGTCCGACTCGCTCTCCCAGAGCGTGTCGCTGATAAGATCTCCGCCCTGCTTGACCGCCGCGACGTCCCTCACAGAGAAACTCCTCTCCATCTCGCTGTCCGGTTAAACGACGCCGTTTCGGCCAAAATCCTCGCCTCCGCCGGCGCCGACGTGTCGCTGCAGAACGCCGCCGGGTGGAACCCCTTGCAGGAGGCTCTCTGCCGCCGGAGCTCCGAGATCGCCTTGATCTTGCTCAAGCTCCACCACCGCTCCGCCTGGGCCAAGTGGCGCCGCCGCCTGCCACGTGTCATCGCGGTGCTGCGGAGAATGCGGGACTTTTACATGGAGATCTCGTTCCACTTCGAGAGCTCCGTTATTCCGTTCGTCGGGAAAATCGCTCCGTCCGACACGTACAAGATCTGGAAGCGCGACGGGAACCTACGCGCCGACACATCGTTGGCCGGGTTCGACGGCCTGAAGATCCAGCGCGCCGATCAGAGCTTCCTCTTCCTCGGCGACGGCGACCAGGCTCACGACGTTCCGGCGGGGTCACTGCTCGTCCTCAACCGCGACGACCGGAAAATCTTCGACGCCTTCGAGAACGCCGGGTCGCCGATGACCGAGTCCGACATCGCCGGATTCTGCTCCCAGACGAGCGTCTACCGCCCCGGCATGGACGTCACCAGGGCCGAGCTCGTCGGCCGGACCAATTGGCGACGGCAGGAGAAGACCGAATCCGTCGGAGAGTGGAAGGCCAGAGTCTTCGAAATGCACAACGTCGTCTTCAGCTTCCGGTCCAGGAAAGTCGCCAATCCCGATAATGCCGACGTGGCGGCGGGGAGCGAGCAGGTCCTCCCTCTAGAGCTAGACGAAGACGACGACGGTTTCTTAGTGGCGGAGAATCCGAGCATGTTCGGAATCCCCGACGGCCGGAGGCACAGTAGCTTCGTCAGAGAAGACCGGGAGTTCGTGGCATTGGGGAGAAAAAGCGTCGACGTGACGTTTCAGCCGCCGCGGAGGTCCATGGCGGCGACCGTTACGGCGGCGCCGGTCCAGACCAAAGAGAAAGAGTACGTCAAGAGCCTGAGACCCTCCGTTTGGTTGACGGACCATTTTCCGTTAAAGACGGAGGAGCTGCTGCCGTTACTGGATATTCTGGCGAGTAAAGTGAAGGCGGTTAGGAGAATGAGGGAGCTGCTCACTACCAAGTTCCCGCCAGGGACGTTTCCGGTGAAGCTGGCGATACCGGTGGTGCCCACAGTGAGAGTGGTGATAACATTCACCAAGTTCGTTGAGCTACAGCCAACGGAGCAGTTCTTCACTCCAATGTCGAGCCCTAGGCAGTTGATAAGGGGCCAACAACAGCAAGAGGAGGAGCACAGGAGCAAGACGGCAACGACGACGCAGAAGCCTTCTGGGTCGTCGTGGTTGAGGCGGAACAGCAGCCAGTCCGCATCGGCCACGAGCAAGCAGCAACACCAGCATTCGAGTGCGGTGGGGTGGCAGGAGCCGGAGCCTTTTGCCATTCCTAGCGGTTATACGTGGACTAGTATCGATGACAAGTCTCGGAAGATGAGGAAATCGAAATCAATTAGGAAATCTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006621 GO:0008104 GO:0008150 GO:0009889 GO:0009987 GO:0010556 GO:0010869 GO:0010941 GO:0012505 GO:0019222 GO:0031323 GO:0032507 GO:0033036 GO:0033365 GO:0034613 GO:0035437 GO:0042981 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045185 GO:0048471 GO:0050789 GO:0050794 GO:0051179 GO:0051235 GO:0051641 GO:0051651 GO:0060255 GO:0065007 GO:0065008 GO:0070727 GO:0070972 GO:0072595 GO:2000209

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 GPCR_chapero_1

Protein Analysis

585

Amino Acids

65.75

Weight (kDa)

9.86

Isoelectric Point (pI)

56.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	81 - 136	6e-06	Ankyrin repeats (3 copies)
ANKRD13_C	PF11904	198 - 564	2.1e-102	ANKRD13 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62050
fragaria_vesca	FvH4_3g01950
malus_domestica	MD05G1347900.v1.1 MD10G1322300.v1.1
prunus_persica	Prupe.4G019600_v2.0.a1
pyrus_communis	pycom05g31540 pycom10g27280
rosa_chinensis	RchiOBHm_Chr5g0002861
rosa_laevigata	RLG00000031066
rosa_multiflora	Rmu_sc0013603.1_g000009 Rmu_sc0020722.1_g000002
rosa_roxburghii	Rroxscaffold_1G00072990
rosa_rugosa	Rorug04G0400300
rosa_samantha	Rh5AG023200 Rh5BG025900 Rh5CG025800 Rh5DG024300
rosa_wichuraiana	Rw5G002160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	237, 831
Acc36I	ACCTGC	1 cut(s)	988
AccB1I	GGYRCC	4 cut(s)	314, 438, 1195, 1402
AccBSI	CCGCTC	1 cut(s)	420
AccI	GTMKAC	2 cut(s)	810, 1143
AccII	CGCG	8 cut(s)	78, 231, 464, 579, 594, 639, 720, 1165
AclWI	GGATC	3 cut(s)	144, 155, 625
AcoI	YGGCCR	5 cut(s)	291, 609, 850, 1078, 1608
AcuI	CTGAAG	2 cut(s)	647, 919
AcvI	CACGTG	1 cut(s)	455
AcyI	GRCGYC	8 cut(s)	35, 234, 282, 315, 439, 741, 828, 1196
AfaI	GTAC	2 cut(s)	563, 1223
AfiI	CCNNNNNNNGG	6 cut(s)	345, 358, 536, 823, 1080, 1248
AflIII	ACRYGT	3 cut(s)	321, 454, 558
AgeI	ACCGGT	1 cut(s)	1396
AhlI	ACTAGT	1 cut(s)	1697
AjiI	CACGTC	3 cut(s)	322, 979, 1147
AjnI	CCWGG	5 cut(s)	425, 675, 833, 947, 1366
AloI	GAACNNNNNNTCC	2 cut(s)	488, 520
Alw21I	GWGCWC	4 cut(s)	383, 521, 846, 1530
Alw26I	GTCTC	3 cut(s)	133, 1231, 1719
AlwI	GGATC	3 cut(s)	144, 155, 625
ApeKI	GCWGC	7 cut(s)	328, 469, 1285, 1288, 1345, 1593, 1621
ArsI	GACNNNNNNTTYG	5 cut(s)	31, 1100, 1132, 1230, 1262
AsiGI	ACCGGT	1 cut(s)	1396
Asp700I	GAANNNNTTC	1 cut(s)	734
AspA2I	CCTAGG	1 cut(s)	1487
AspLEI	GCGC	7 cut(s)	317, 441, 579, 596, 639, 641, 1198
AspS9I	GGNCC	9 cut(s)	429, 837, 855, 945, 1000, 1170, 1201, 1258, 1504
AsuC2I	CCSGG	5 cut(s)	343, 613, 757, 819, 1112
AsuHPI	GGTGA	4 cut(s)	823, 1393, 1423, 1432
AsuII	TTCGAA	1 cut(s)	915
AvaII	GGWCC	6 cut(s)	855, 945, 1000, 1170, 1201, 1258
AvrII	CCTAGG	1 cut(s)	1487
BaeGI	GKGCMC	1 cut(s)	1407
BanI	GGYRCC	4 cut(s)	314, 438, 1195, 1402
BanII	GRGCYC	4 cut(s)	383, 521, 846, 1487
BauI	CACGAG	1 cut(s)	1612
BbrPI	CACGTG	1 cut(s)	455
BbsI	GAAGAC	5 cut(s)	884, 904, 925, 1029, 1113
Bbv12I	GWGCWC	4 cut(s)	383, 521, 846, 1530
BbvI	GCAGC	7 cut(s)	315, 456, 1272, 1275, 1332, 1605, 1633
BccI	CCATC	1 cut(s)	267
BceAI	ACGGC	9 cut(s)	48, 269, 637, 685, 884, 1093, 1206, 1276, 1557
BcgI	CGANNNNNNTGC	1 cut(s)	28
BciT130I	CCWGG	5 cut(s)	427, 677, 835, 949, 1368
BcnI	CCSGG	5 cut(s)	343, 613, 757, 819, 1112
BcoDI	GTCTC	3 cut(s)	133, 1231, 1719
BcuI	ACTAGT	1 cut(s)	1697
BfaI	CTAG	5 cut(s)	1010, 1016, 1488, 1680, 1698
BfmI	CTRYAG	2 cut(s)	329, 1448
BfoI	RGCGCY	3 cut(s)	318, 442, 1199
BfuAI	ACCTGC	1 cut(s)	988
BglI	GCCNNNNNGGC	3 cut(s)	153, 290, 980
BglII	AGATCT	3 cut(s)	207, 498, 567
BlnI	CCTAGG	1 cut(s)	1487
Bme18I	GGWCC	6 cut(s)	855, 945, 1000, 1170, 1201, 1258
BmgBI	CACGTC	3 cut(s)	322, 979, 1147
BmgT120I	GGNCC	9 cut(s)	429, 837, 855, 945, 1000, 1170, 1201, 1258, 1504
BmiI	GGNNCC	9 cut(s)	316, 350, 440, 587, 1197, 1404, 1505, 1659, 1665
BmsI	GCATC	1 cut(s)	1613
BoxI	GACNNNNGTC	1 cut(s)	806
BpiI	GAAGAC	5 cut(s)	884, 904, 925, 1029, 1113
BplI	GAGNNNNNCTC	2 cut(s)	162, 194
Bpu14I	TTCGAA	1 cut(s)	915
BpuEI	CTTGAG	2 cut(s)	35, 389
BpuMI	CCSGG	5 cut(s)	343, 613, 757, 819, 1112
Bsa29I	ATCGAT	1 cut(s)	1704
BsaAI	YACGTR	3 cut(s)	455, 561, 1692
BsaHI	GRCGYC	8 cut(s)	35, 234, 282, 315, 439, 741, 828, 1196
BsaI	GGTCTC	1 cut(s)	1231
BsaJI	CCNNGG	8 cut(s)	426, 661, 817, 834, 1163, 1173, 1367, 1487
BsaWI	WCCGGW	5 cut(s)	270, 726, 942, 1378, 1396
Bsc4I	CCNNNNNNNGG	6 cut(s)	345, 358, 536, 823, 1080, 1248
Bse118I	RCCGGY	3 cut(s)	311, 1198, 1396
Bse1I	ACTGG	2 cut(s)	1302, 1597
BseBI	CCWGG	5 cut(s)	427, 677, 835, 949, 1368
BseCI	ATCGAT	1 cut(s)	1704
BseDI	CCNNGG	8 cut(s)	426, 661, 817, 834, 1163, 1173, 1367, 1487
BseLI	CCNNNNNNNGG	6 cut(s)	345, 358, 536, 823, 1080, 1248
BseMII	CTCAG	2 cut(s)	171, 1226
BseNI	ACTGG	2 cut(s)	1302, 1597
BsePI	GCGCGC	1 cut(s)	637
BseRI	GAGGAG	5 cut(s)	16, 243, 1295, 1535, 1538
BseSI	GKGCMC	1 cut(s)	1407
BseX3I	CGGCCG	2 cut(s)	850, 1078
BseXI	GCAGC	7 cut(s)	315, 456, 1272, 1275, 1332, 1605, 1633
Bsh1236I	CGCG	8 cut(s)	78, 231, 464, 579, 594, 639, 720, 1165
Bsh1285I	CGRYCG	4 cut(s)	727, 853, 1081, 1185
BshNI	GGYRCC	4 cut(s)	314, 438, 1195, 1402
BshTI	ACCGGT	1 cut(s)	1396
BshVI	ATCGAT	1 cut(s)	1704
BsiEI	CGRYCG	4 cut(s)	727, 853, 1081, 1185
BsiHKAI	GWGCWC	4 cut(s)	383, 521, 846, 1530
BslFI	GGGAC	3 cut(s)	221, 497, 1384
BslI	CCNNNNNNNGG	6 cut(s)	345, 358, 536, 823, 1080, 1248
BsmAI	GTCTC	3 cut(s)	133, 1231, 1719
BsmBI	CGTCTC	1 cut(s)	133
BsmFI	GGGAC	3 cut(s)	221, 497, 1384
BsmI	GAATGC	2 cut(s)	483, 1633
Bso31I	GGTCTC	1 cut(s)	1231
Bsp119I	TTCGAA	1 cut(s)	915
Bsp1286I	GDGCHC	6 cut(s)	383, 521, 846, 1407, 1487, 1530
Bsp143I	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
Bsp19I	CCATGG	1 cut(s)	1173
BspCNI	CTCAG	2 cut(s)	170, 1227
BspDI	ATCGAT	1 cut(s)	1704
BspFNI	CGCG	8 cut(s)	78, 231, 464, 579, 594, 639, 720, 1165
BspLI	GGNNCC	9 cut(s)	316, 350, 440, 587, 1197, 1404, 1505, 1659, 1665
BspMAI	CTGCAG	1 cut(s)	333
BspMI	ACCTGC	1 cut(s)	988
BspPI	GGATC	3 cut(s)	144, 155, 625
BspT104I	TTCGAA	1 cut(s)	915
BspT107I	GGYRCC	4 cut(s)	314, 438, 1195, 1402
BspTNI	GGTCTC	1 cut(s)	1231
BsrBI	CCGCTC	1 cut(s)	420
BsrFI	RCCGGY	3 cut(s)	311, 1198, 1396
BsrI	ACTGG	2 cut(s)	1302, 1597
BssAI	RCCGGY	3 cut(s)	311, 1198, 1396
BssECI	CCNNGG	8 cut(s)	426, 661, 817, 834, 1163, 1173, 1367, 1487
BssHII	GCGCGC	1 cut(s)	637
BssMI	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
BssNI	GRCGYC	8 cut(s)	35, 234, 282, 315, 439, 741, 828, 1196
BssSI	CACGAG	1 cut(s)	1612
BssT1I	CCWWGG	2 cut(s)	1173, 1487
Bst2BI	CACGAG	1 cut(s)	1612
Bst2UI	CCWGG	5 cut(s)	427, 677, 835, 949, 1368
Bst4CI	ACNGT	5 cut(s)	91, 1034, 1091, 1186, 1411
Bst6I	CTCTTC	1 cut(s)	662
BstACI	GRCGYC	8 cut(s)	35, 234, 282, 315, 439, 741, 828, 1196
BstBAI	YACGTR	3 cut(s)	455, 561, 1692
BstBI	TTCGAA	1 cut(s)	915
BstC8I	GCNNGC	6 cut(s)	313, 449, 639, 995, 1389, 1619
BstDEI	CTNAG	4 cut(s)	157, 1039, 1235, 1752
BstDSI	CCRYGG	2 cut(s)	1163, 1173
BstFNI	CGCG	8 cut(s)	78, 231, 464, 579, 594, 639, 720, 1165
BstH2I	RGCGCY	3 cut(s)	318, 442, 1199
BstHHI	GCGC	7 cut(s)	317, 441, 579, 596, 639, 641, 1198
BstKTI	GATC	9 cut(s)	152, 163, 210, 390, 399, 501, 570, 633, 646
BstMAI	GTCTC	3 cut(s)	133, 1231, 1719
BstMBI	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
BstMCI	CGRYCG	4 cut(s)	727, 853, 1081, 1185
BstNI	CCWGG	5 cut(s)	427, 677, 835, 949, 1368
BstNSI	RCATGY	1 cut(s)	1063
BstPAI	GACNNNNGTC	1 cut(s)	806
BstSFI	CTRYAG	2 cut(s)	329, 1448
BstSLI	GKGCMC	1 cut(s)	1407
BstUI	CGCG	8 cut(s)	78, 231, 464, 579, 594, 639, 720, 1165
BstV1I	GCAGC	7 cut(s)	315, 456, 1272, 1275, 1332, 1605, 1633
BstV2I	GAAGAC	5 cut(s)	884, 904, 925, 1029, 1113
BstX2I	RGATCY	5 cut(s)	160, 207, 498, 567, 630
BstYI	RGATCY	5 cut(s)	160, 207, 498, 567, 630
BstZI	CGGCCG	2 cut(s)	850, 1078
Bsu15I	ATCGAT	1 cut(s)	1704
BsuTUI	ATCGAT	1 cut(s)	1704
BtgI	CCRYGG	2 cut(s)	1163, 1173
BtgZI	GCGATG	2 cut(s)	445, 766
BtrI	CACGTC	3 cut(s)	322, 979, 1147
BtsI	GCAGTG	1 cut(s)	701
BtsIMutI	CAGTG	2 cut(s)	701, 1416
BveI	ACCTGC	1 cut(s)	988
Cac8I	GCNNGC	6 cut(s)	313, 449, 639, 995, 1389, 1619
CfoI	GCGC	7 cut(s)	317, 441, 579, 596, 639, 641, 1198
Cfr10I	RCCGGY	3 cut(s)	311, 1198, 1396
Cfr13I	GGNCC	9 cut(s)	429, 837, 855, 945, 1000, 1170, 1201, 1258, 1504
Cfr42I	CCGCGG	1 cut(s)	1166
ClaI	ATCGAT	1 cut(s)	1704
CseI	GACGC	7 cut(s)	24, 67, 290, 749, 796, 1129, 1561
Csp6I	GTAC	2 cut(s)	562, 1222
CspAI	ACCGGT	1 cut(s)	1396
CviAII	CATG	4 cut(s)	493, 823, 1060, 1174
CviQI	GTAC	2 cut(s)	562, 1222
DdeI	CTNAG	4 cut(s)	157, 1039, 1235, 1752
DinI	GGCGCC	3 cut(s)	316, 440, 1197
DpnI	GATC	9 cut(s)	151, 162, 209, 389, 398, 500, 569, 632, 645
DpnII	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
EaeI	YGGCCR	5 cut(s)	291, 609, 850, 1078, 1608
EagI	CGGCCG	2 cut(s)	850, 1078
Eam1104I	CTCTTC	1 cut(s)	662
EarI	CTCTTC	1 cut(s)	662
EciI	GGCGGA	6 cut(s)	33, 202, 298, 412, 1061, 1600
Ecl136II	GAGCTC	3 cut(s)	381, 519, 844
EclXI	CGGCCG	2 cut(s)	850, 1078
Eco130I	CCWWGG	2 cut(s)	1173, 1487
Eco24I	GRGCYC	4 cut(s)	383, 521, 846, 1487
Eco31I	GGTCTC	1 cut(s)	1231
Eco47I	GGWCC	6 cut(s)	855, 945, 1000, 1170, 1201, 1258
Eco52I	CGGCCG	2 cut(s)	850, 1078
Eco53kI	GAGCTC	3 cut(s)	381, 519, 844
Eco57I	CTGAAG	2 cut(s)	647, 919
Eco72I	CACGTG	1 cut(s)	455
EcoICRI	GAGCTC	3 cut(s)	381, 519, 844
EcoO109I	RGGNCCY	1 cut(s)	1000
EcoRII	CCWGG	5 cut(s)	425, 675, 833, 947, 1366
EcoT14I	CCWWGG	2 cut(s)	1173, 1487
EcoT38I	GRGCYC	4 cut(s)	383, 521, 846, 1487
EgeI	GGCGCC	3 cut(s)	316, 440, 1197
EheI	GGCGCC	3 cut(s)	316, 440, 1197
ErhI	CCWWGG	2 cut(s)	1173, 1487
Esp3I	CGTCTC	1 cut(s)	133
FaeI	CATG	4 cut(s)	496, 826, 1063, 1177
FaiI	YATR	5 cut(s)	494, 824, 1061, 1175, 1689
FaqI	GGGAC	3 cut(s)	221, 497, 1384
FatI	CATG	4 cut(s)	492, 822, 1059, 1173
FauI	CCCGC	5 cut(s)	147, 474, 688, 979, 1371
FblI	GTMKAC	2 cut(s)	810, 1143
FriOI	GRGCYC	4 cut(s)	383, 521, 846, 1487
FspBI	CTAG	5 cut(s)	1010, 1016, 1488, 1680, 1698
GlaI	GCGC	7 cut(s)	316, 440, 578, 595, 638, 640, 1197
HaeII	RGCGCY	3 cut(s)	318, 442, 1199
HgaI	GACGC	7 cut(s)	24, 67, 290, 749, 796, 1129, 1561
HhaI	GCGC	7 cut(s)	317, 441, 579, 596, 639, 641, 1198
Hin1I	GRCGYC	8 cut(s)	35, 234, 282, 315, 439, 741, 828, 1196
Hin1II	CATG	4 cut(s)	496, 826, 1063, 1177
Hin6I	GCGC	7 cut(s)	315, 439, 577, 594, 637, 639, 1196
HinP1I	GCGC	7 cut(s)	315, 439, 577, 594, 637, 639, 1196
HincII	GTYRAC	2 cut(s)	1144, 1254
HindII	GTYRAC	2 cut(s)	1144, 1254
HinfI	GANTC	9 cut(s)	123, 171, 177, 773, 789, 884, 909, 1051, 1068
HphI	GGTGA	4 cut(s)	823, 1393, 1423, 1432
Hpy166II	GTNNAC	5 cut(s)	82, 811, 1144, 1254, 1695
Hpy8I	GTNNAC	5 cut(s)	82, 811, 1144, 1254, 1695
HpyAV	CCTTC	4 cut(s)	754, 895, 1315, 1572
HpyCH4III	ACNGT	5 cut(s)	91, 1034, 1091, 1186, 1411
HpyCH4V	TGCA	3 cut(s)	331, 358, 922
HpyF3I	CTNAG	4 cut(s)	157, 1039, 1235, 1752
Hsp92I	GRCGYC	8 cut(s)	35, 234, 282, 315, 439, 741, 828, 1196
Hsp92II	CATG	4 cut(s)	496, 826, 1063, 1177
HspAI	GCGC	7 cut(s)	315, 439, 577, 594, 637, 639, 1196
KasI	GGCGCC	3 cut(s)	314, 438, 1195
KroI	GCCGGC	1 cut(s)	311
KroNI	GCCGGC	1 cut(s)	313
KspI	CCGCGG	1 cut(s)	1166
Kzo9I	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
Lsp1109I	GCAGC	7 cut(s)	315, 456, 1272, 1275, 1332, 1605, 1633
LweI	GCATC	1 cut(s)	1613
MaeI	CTAG	5 cut(s)	1010, 1016, 1488, 1680, 1698
MaeIII	GTNAC	5 cut(s)	699, 829, 1147, 1186, 1293
MalI	GATC	9 cut(s)	151, 162, 209, 389, 398, 500, 569, 632, 645
MbiI	CCGCTC	1 cut(s)	420
MboI	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
MfeI	CAATTG	1 cut(s)	859
MflI	RGATCY	5 cut(s)	160, 207, 498, 567, 630
MhlI	GDGCHC	6 cut(s)	383, 521, 846, 1407, 1487, 1530
MluCI	AATT	2 cut(s)	859, 1740
Mly113I	GGCGCC	3 cut(s)	315, 439, 1196
MlyI	GAGTC	4 cut(s)	171, 180, 782, 918
MmeI	TCCRAC	4 cut(s)	199, 579, 801, 871
MreI	CGCCGGCG	1 cut(s)	311
MroNI	GCCGGC	1 cut(s)	311
MroXI	GAANNNNTTC	1 cut(s)	734
MseI	TTAA	2 cut(s)	275, 1271
MslI	CAYNNNNRTG	3 cut(s)	464, 1475, 1638
MspA1I	CMGCKG	1 cut(s)	1165
MunI	CAATTG	1 cut(s)	859
Mva1269I	GAATGC	2 cut(s)	483, 1633
MvaI	CCWGG	5 cut(s)	427, 677, 835, 949, 1368
MvnI	CGCG	8 cut(s)	78, 231, 464, 579, 594, 639, 720, 1165
NaeI	GCCGGC	1 cut(s)	313
NarI	GGCGCC	3 cut(s)	315, 439, 1196
NciI	CCSGG	5 cut(s)	343, 613, 757, 819, 1112
NcoI	CCATGG	1 cut(s)	1173
NdeII	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
NgoMIV	GCCGGC	1 cut(s)	311
NlaIII	CATG	4 cut(s)	496, 826, 1063, 1177
NlaIV	GGNNCC	9 cut(s)	316, 350, 440, 587, 1197, 1404, 1505, 1659, 1665
NmeAIII	GCCGAG	2 cut(s)	642, 865
NmuCI	GTSAC	3 cut(s)	699, 829, 1147
NspI	RCATGY	1 cut(s)	1063
NspV	TTCGAA	1 cut(s)	915
PauI	GCGCGC	1 cut(s)	637
PctI	GAATGC	2 cut(s)	483, 1633
PdiI	GCCGGC	1 cut(s)	313
PdmI	GAANNNNTTC	1 cut(s)	734
PfeI	GAWTC	5 cut(s)	123, 789, 884, 1051, 1068
PflFI	GACNNNGTC	2 cut(s)	34, 772
PfoI	TCCNGGA	1 cut(s)	947
PinAI	ACCGGT	1 cut(s)	1396
PleI	GAGTC	4 cut(s)	171, 179, 781, 917
PluTI	GGCGCC	3 cut(s)	318, 442, 1199
PmaCI	CACGTG	1 cut(s)	455
PmlI	CACGTG	1 cut(s)	455
PpsI	GAGTC	4 cut(s)	171, 179, 781, 917
Ppu21I	YACGTR	3 cut(s)	455, 561, 1692
PpuMI	RGGWCCY	1 cut(s)	1000
PshAI	GACNNNNGTC	1 cut(s)	806
Psp124BI	GAGCTC	3 cut(s)	383, 521, 846
Psp5II	RGGWCCY	1 cut(s)	1000
Psp6I	CCWGG	5 cut(s)	425, 675, 833, 947, 1366
PspCI	CACGTG	1 cut(s)	455
PspGI	CCWGG	5 cut(s)	425, 675, 833, 947, 1366
PspN4I	GGNNCC	9 cut(s)	316, 350, 440, 587, 1197, 1404, 1505, 1659, 1665
PspPI	GGNCC	9 cut(s)	429, 837, 855, 945, 1000, 1170, 1201, 1258, 1504
PspPPI	RGGWCCY	1 cut(s)	1000
PstI	CTGCAG	1 cut(s)	333
PsuI	RGATCY	5 cut(s)	160, 207, 498, 567, 630
PsyI	GACNNNGTC	2 cut(s)	34, 772
PteI	GCGCGC	1 cut(s)	637
RsaI	GTAC	2 cut(s)	563, 1223
RsaNI	GTAC	2 cut(s)	562, 1222
RseI	CAYNNNNRTG	3 cut(s)	464, 1475, 1638
SacI	GAGCTC	3 cut(s)	383, 521, 846
SacII	CCGCGG	1 cut(s)	1166
SalI	GTCGAC	1 cut(s)	1142
SaqAI	TTAA	2 cut(s)	275, 1271
Sau3AI	GATC	9 cut(s)	149, 160, 207, 387, 396, 498, 567, 630, 643
Sau96I	GGNCC	9 cut(s)	429, 837, 855, 945, 1000, 1170, 1201, 1258, 1504
SchI	GAGTC	4 cut(s)	171, 180, 782, 918
SduI	GDGCHC	6 cut(s)	383, 521, 846, 1407, 1487, 1530
SfaNI	GCATC	1 cut(s)	1613
SfcI	CTRYAG	2 cut(s)	329, 1448
SfoI	GGCGCC	3 cut(s)	316, 440, 1197
Sfr303I	CCGCGG	1 cut(s)	1166
SfuI	TTCGAA	1 cut(s)	915
SgrAI	CRCCGGYG	1 cut(s)	311
SgrBI	CCGCGG	1 cut(s)	1166
SgrDI	CGTCGACG	1 cut(s)	1142
SinI	GGWCC	6 cut(s)	855, 945, 1000, 1170, 1201, 1258
SmiMI	CAYNNNNRTG	3 cut(s)	464, 1475, 1638
SmlI	CTYRAG	2 cut(s)	50, 404
SmoI	CTYRAG	2 cut(s)	50, 404
SpeI	ACTAGT	1 cut(s)	1697
Sse9I	AATT	2 cut(s)	859, 1740
SspDI	GGCGCC	3 cut(s)	314, 438, 1195
SspMI	CTAG	5 cut(s)	1010, 1016, 1488, 1680, 1698
SstI	GAGCTC	3 cut(s)	383, 521, 846
StyI	CCWWGG	2 cut(s)	1173, 1487
TaaI	ACNGT	5 cut(s)	91, 1034, 1091, 1186, 1411
TaqII	GACCGA	2 cut(s)	785, 896
TasI	AATT	2 cut(s)	859, 1740
TfiI	GAWTC	5 cut(s)	123, 789, 884, 1051, 1068
Tru1I	TTAA	2 cut(s)	275, 1271
Tru9I	TTAA	2 cut(s)	275, 1271
TscAI	CASTG	2 cut(s)	708, 1416
TseFI	GTSAC	3 cut(s)	699, 829, 1147
TseI	GCWGC	7 cut(s)	328, 469, 1285, 1288, 1345, 1593, 1621
Tsp45I	GTSAC	3 cut(s)	699, 829, 1147
TspGWI	ACGGA	9 cut(s)	511, 519, 540, 877, 1234, 1257, 1271, 1292, 1472
TspRI	CASTG	2 cut(s)	708, 1416
Tth111I	GACNNNGTC	2 cut(s)	34, 772
VpaK11BI	GGWCC	6 cut(s)	855, 945, 1000, 1170, 1201, 1258
XbaI	TCTAGA	1 cut(s)	1009
XceI	RCATGY	1 cut(s)	1063
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1414
XmaJI	CCTAGG	1 cut(s)	1487
XmiI	GTMKAC	2 cut(s)	810, 1143
XmnI	GAANNNNTTC	1 cut(s)	734
XspI	CTAG	5 cut(s)	1010, 1016, 1488, 1680, 1698
ZraI	GACGTC	2 cut(s)	235, 829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.