RLG00000031066

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013140
Physical Location & Seq
Forward (+)
1692383 .. 1696456
4074 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031066

Sequence Viewer

Length: 2043 bp
ATGGCAAAGTCGTCGTCGTCCTCGGCGGCGTCGTTTCCGCCTCTCAAGCCCGACGACTACTCCCACAGCCGCGTCCACTACGCCGTCGTTTTGGCAGACCACACCACTCTCTCCCGAATCGTCTCCACGCTCCCCCGCCTCGCCGATCCGTCTCAGATCCACACCGAGTCCGACTCGCTCTCCCAGGAGCGTGTCGCTGATAAGATCTCCGCCGTGCTCGACCGCCGCGACGTCCCTCACAGAGAAACCCCTCTCCATCTTGCTGTCCGGTTAAACGACGCCGTTTCGGCCAAAATCCTCGCCTCCGCCGGCGCCGACGTGTCGCTGCAGAACGCCGCCGGGTGGAACCCCTTGCAGGAGGCTCTCTGCCGCCGGAGCTCAGAGATCGCCTTGATCTTGCTCAAGCTCCACCACCGCTCCGCCTGGGCCAAGTGGCGCCGCCGCCTGCCACGTGTCATCGCGGTTCTGCGGAGAATGCGGGACTTCTACATGGAGATCTCGTTCCACTTCGAGAGCTCCGTCATTCCGTTCGTCGGGAAAATTGCTCCGTCCGATACGTACAAGATCTGGAAGCGCGACGGGAACCTACGCGCCGACACGTCGTTGGCCGGGTTCGACGGCCTGAAGATCCAGCGCGCCGATCAGAGCTTCCTCTTCCTCGGCGACGGAGACCAGACTCACGACGTTCCGGCGGGGTCACTGCTCGTCCTCAACCGCGACGACCGGAAAATCTTCGACGCCTTCGAGAACGCCGGGTCGCCGATGACCGAGTCCGACATCGCCGGATTCTGCTCCCAGACTAGCGTCTACCGCCCCGGCATGGACGTCACCAGAGCCGAGCTCGTCGGCCGGACCAATTGGCGACGGCAGGAGAAGACCGAATCCGTCGGAGAGTGGAAGGCCAGAGTCTTCGAAATGCACAACGTCGTCTTCAGCTTCCGGTCCAGGAAAGTCGCCAATCCTGATAATGCTGACGTGGCGGCGGGGAGCGAGCAGGTGCTCCCTCTAGAGCTAGACGAAGACGACGACGGTTTTTTAGTGGCGGAGAATCCGAGCATGTTCGGAATCCCCGACGGCCGGAGGCACAGTAGCTTCGTCAGAGAGGACCGGGAGTTCGTGGCATTGGGGAGAAAAAGCGTCGACGTGACATTTCAGCCGCCGCGGAGGTCCATGGCGGCGACCGTTACGGCGGCGCCGGTCCAGACGAAAGAGAAAGAGTACGTCAAGAGCCTGAGACCCTCCGTTTGGCTAACGGACCATTTTCCGTTAAAGACGGAGGAGCTGCTGCCGTTACTGGATATTCTGGCGAGTAAAGTGAAGGCGGTTAGGAGAATGAGGGAGCTGCTCACTACCAAGTTCCCGCCAGGGACGTTTCCGGTGAAGCTGGCGATACCGGTGGTGCCCACAGTGAGAGTGGTGATAACATTCACCAAGTTCGTTGAGCTACAACCAACCGAGCGGTTCTTCACTCCAATGTCGAGCCCTAGGCAGTTGTTAAGGGGACAACAGCAAGAGGAGGAGCACAAGAGCAAGACGACGACGACGACGACGACGCAGAAGGTTTCTGGGTCGTCGTGGTTGAGGCGGAACAGCAGTCAGTCCGCGTCGGCCACGAGCAAGCAGCAACACCAGCATTCTGCTGCGGCGGGGTGGCAGGAGCCGGAGCCTTTTGCCATTCCTAGTGGGTCTCTCTCTCTCTCCAATTCTTATTGCTTGGCAACCCTCCTTTGCTTAAACTACTCTTTCATCTTTCTGAATCTGTACGCTAACAATCTCCAGGGTAGTGAAATGGCAACCGAAGCACCGGGGTGGGCGGATCAATGGGGCGCCGGAGGCATCGGTGCATTGGAAGATGAAGACAAGAACAAAAGCCTGGAAGACAACAACAAGAAGAAGGGAGAGACCAAGTCTGGACTAAACAAAGCGAAGGTTGCAGCAATGGCTGGCGCTGAAAAGATCAAAAGTGGCACAACCAATGGCATCAAATGGATCAAAAATCAGTGCCAGAAGAAGAAATCTACCAAGACCCCAACTGAAGTTTAA

Protein Analysis

681

Amino Acids

75.6

Weight (kDa)

9.29

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 197 - 562 3e-100 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62050
fragaria_vesca FvH4_3g01950
malus_domestica MD05G1347900.v1.1 MD10G1322300.v1.1
prunus_persica Prupe.4G019600_v2.0.a1
pyrus_communis pycom05g31540 pycom10g27280
rosa_chinensis RchiOBHm_Chr5g0002861
rosa_laevigata RLG00000031066
rosa_multiflora Rmu_sc0013603.1_g000009 Rmu_sc0020722.1_g000002
rosa_roxburghii Rroxscaffold_1G00072990
rosa_rugosa Rorug04G0400300
rosa_samantha Rh5AG023200 Rh5BG025900 Rh5CG025800 Rh5DG024300
rosa_wichuraiana Rw5G002160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 985
AatII GACGTC 2 cut(s) 234, 828
Acc36I ACCTGC 1 cut(s) 985
AccB1I GGYRCC 5 cut(s) 311, 435, 1192, 1399, 1826
AccBSI CCGCTC 2 cut(s) 417, 1459
AccI GTMKAC 2 cut(s) 807, 1140
AccII CGCG 9 cut(s) 72, 228, 461, 576, 591, 636, 717, 1162, 1604
AclWI GGATC 5 cut(s) 140, 151, 622, 1824, 1997
AcoI YGGCCR 5 cut(s) 288, 606, 847, 1075, 1608
AcuI CTGAAG 2 cut(s) 644, 916
AcvI CACGTG 1 cut(s) 452
AcyI GRCGYC 9 cut(s) 29, 231, 279, 312, 436, 738, 825, 1193, 1827
AfaI GTAC 3 cut(s) 560, 1220, 1763
AfiI CCNNNNNNNGG 6 cut(s) 342, 355, 533, 820, 1077, 1245
AflIII ACRYGT 3 cut(s) 318, 451, 597
AgeI ACCGGT 1 cut(s) 1393
AjiI CACGTC 4 cut(s) 319, 600, 976, 1144
AjnI CCWGG 6 cut(s) 183, 422, 944, 1363, 1776, 1872
AleI CACNNNNGTG 1 cut(s) 1807
AloI GAACNNNNNNTCC 4 cut(s) 485, 517, 1097, 1129
Alw21I GWGCWC 6 cut(s) 219, 380, 518, 843, 1002, 1524
Alw26I GTCTC 6 cut(s) 127, 156, 663, 1228, 1692, 1895
AlwI GGATC 5 cut(s) 140, 151, 622, 1824, 1997
AoxI GGCC 8 cut(s) 288, 426, 606, 619, 847, 900, 1075, 1608
ApeKI GCWGC 7 cut(s) 325, 1282, 1285, 1342, 1621, 1640, 1934
ArsI GACNNNNNNTTYG 5 cut(s) 31, 1097, 1129, 1227, 1259
AsiGI ACCGGT 1 cut(s) 1393
Asp700I GAANNNNTTC 1 cut(s) 731
AspA2I CCTAGG 1 cut(s) 1484
AspLEI GCGC 9 cut(s) 314, 438, 576, 593, 636, 638, 1195, 1829, 1949
AspS9I GGNCC 7 cut(s) 426, 852, 942, 1105, 1167, 1198, 1255
AsuC2I CCSGG 6 cut(s) 340, 610, 754, 816, 1109, 1806
AsuHPI GGTGA 4 cut(s) 820, 1390, 1420, 1429
AsuII TTCGAA 1 cut(s) 912
AvaII GGWCC 6 cut(s) 852, 942, 1105, 1167, 1198, 1255
AvrII CCTAGG 1 cut(s) 1484
BaeGI GKGCMC 1 cut(s) 1404
BanI GGYRCC 5 cut(s) 311, 435, 1192, 1399, 1826
BanII GRGCYC 4 cut(s) 380, 518, 843, 1484
BauI CACGAG 1 cut(s) 1612
BbrPI CACGTG 1 cut(s) 452
BbsI GAAGAC 6 cut(s) 881, 901, 922, 1026, 1863, 1884
Bbv12I GWGCWC 6 cut(s) 219, 380, 518, 843, 1002, 1524
BbvI GCAGC 7 cut(s) 312, 1269, 1272, 1329, 1627, 1633, 1946
BccI CCATC 1 cut(s) 264
BceAI ACGGC 8 cut(s) 68, 197, 266, 634, 881, 1090, 1203, 1273
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 6 cut(s) 185, 424, 946, 1365, 1778, 1874
BcnI CCSGG 6 cut(s) 340, 610, 754, 816, 1109, 1806
BcoDI GTCTC 6 cut(s) 127, 156, 663, 1228, 1692, 1895
BfaI CTAG 5 cut(s) 801, 1007, 1013, 1485, 1680
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 5 cut(s) 315, 439, 1196, 1830, 1950
BfuAI ACCTGC 1 cut(s) 985
BglI GCCNNNNNGGC 1 cut(s) 287
BglII AGATCT 3 cut(s) 204, 495, 564
BlnI CCTAGG 1 cut(s) 1484
Bme18I GGWCC 6 cut(s) 852, 942, 1105, 1167, 1198, 1255
BmgBI CACGTC 4 cut(s) 319, 600, 976, 1144
BmgT120I GGNCC 7 cut(s) 426, 852, 942, 1105, 1167, 1198, 1255
BmiI GGNNCC 9 cut(s) 313, 347, 437, 584, 1194, 1401, 1659, 1665, 1828
BmsI GCATC 2 cut(s) 1845, 1989
BoxI GACNNNNGTC 1 cut(s) 803
BpiI GAAGAC 6 cut(s) 881, 901, 922, 1026, 1863, 1884
BplI GAGNNNNNCTC 4 cut(s) 158, 190, 825, 857
BpmI CTGGAG 1 cut(s) 1760
Bpu14I TTCGAA 1 cut(s) 912
BpuEI CTTGAG 2 cut(s) 29, 386
BpuMI CCSGG 6 cut(s) 340, 610, 754, 816, 1109, 1806
BsaAI YACGTR 2 cut(s) 452, 558
BsaHI GRCGYC 9 cut(s) 29, 231, 279, 312, 436, 738, 825, 1193, 1827
BsaI GGTCTC 4 cut(s) 663, 1228, 1692, 1895
BsaWI WCCGGW 5 cut(s) 267, 723, 939, 1375, 1393
Bsc4I CCNNNNNNNGG 6 cut(s) 342, 355, 533, 820, 1077, 1245
Bse118I RCCGGY 3 cut(s) 308, 1195, 1393
Bse1I ACTGG 1 cut(s) 1299
Bse3DI GCAATG 1 cut(s) 1944
BseBI CCWGG 6 cut(s) 185, 424, 946, 1365, 1778, 1874
BseLI CCNNNNNNNGG 6 cut(s) 342, 355, 533, 820, 1077, 1245
BseMI GCAATG 1 cut(s) 1944
BseMII CTCAG 3 cut(s) 167, 393, 1223
BseNI ACTGG 1 cut(s) 1299
BsePI GCGCGC 1 cut(s) 634
BseRI GAGGAG 3 cut(s) 1292, 1529, 1532
BseSI GKGCMC 1 cut(s) 1404
BseX3I CGGCCG 2 cut(s) 847, 1075
BseXI GCAGC 7 cut(s) 312, 1269, 1272, 1329, 1627, 1633, 1946
Bsh1236I CGCG 9 cut(s) 72, 228, 461, 576, 591, 636, 717, 1162, 1604
Bsh1285I CGRYCG 5 cut(s) 223, 724, 850, 1078, 1182
BshFI GGCC 8 cut(s) 290, 428, 608, 621, 849, 902, 1077, 1610
BshNI GGYRCC 5 cut(s) 311, 435, 1192, 1399, 1826
BshTI ACCGGT 1 cut(s) 1393
BsiEI CGRYCG 5 cut(s) 223, 724, 850, 1078, 1182
BsiHKAI GWGCWC 6 cut(s) 219, 380, 518, 843, 1002, 1524
BslFI GGGAC 4 cut(s) 218, 494, 1381, 1515
BslI CCNNNNNNNGG 6 cut(s) 342, 355, 533, 820, 1077, 1245
BsmAI GTCTC 6 cut(s) 127, 156, 663, 1228, 1692, 1895
BsmBI CGTCTC 2 cut(s) 127, 156
BsmFI GGGAC 4 cut(s) 218, 494, 1381, 1515
BsmI GAATGC 2 cut(s) 480, 1633
BsnI GGCC 8 cut(s) 290, 428, 608, 621, 849, 902, 1077, 1610
Bso31I GGTCTC 4 cut(s) 663, 1228, 1692, 1895
Bsp119I TTCGAA 1 cut(s) 912
Bsp1286I GDGCHC 8 cut(s) 219, 380, 518, 843, 1002, 1404, 1484, 1524
Bsp19I CCATGG 1 cut(s) 1170
BspANI GGCC 8 cut(s) 290, 428, 608, 621, 849, 902, 1077, 1610
BspCNI CTCAG 3 cut(s) 166, 392, 1224
BspFNI CGCG 9 cut(s) 72, 228, 461, 576, 591, 636, 717, 1162, 1604
BspLI GGNNCC 9 cut(s) 313, 347, 437, 584, 1194, 1401, 1659, 1665, 1828
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 985
BspPI GGATC 5 cut(s) 140, 151, 622, 1824, 1997
BspT104I TTCGAA 1 cut(s) 912
BspT107I GGYRCC 5 cut(s) 311, 435, 1192, 1399, 1826
BspTNI GGTCTC 4 cut(s) 663, 1228, 1692, 1895
BsrBI CCGCTC 2 cut(s) 417, 1459
BsrDI GCAATG 1 cut(s) 1944
BsrFI RCCGGY 3 cut(s) 308, 1195, 1393
BsrI ACTGG 1 cut(s) 1299
BssAI RCCGGY 3 cut(s) 308, 1195, 1393
BssHII GCGCGC 1 cut(s) 634
BssNI GRCGYC 9 cut(s) 29, 231, 279, 312, 436, 738, 825, 1193, 1827
BssSI CACGAG 1 cut(s) 1612
BssT1I CCWWGG 2 cut(s) 1170, 1484
Bst2BI CACGAG 1 cut(s) 1612
Bst2UI CCWGG 6 cut(s) 185, 424, 946, 1365, 1778, 1874
Bst4CI ACNGT 4 cut(s) 1031, 1088, 1183, 1408
Bst6I CTCTTC 1 cut(s) 659
BstACI GRCGYC 9 cut(s) 29, 231, 279, 312, 436, 738, 825, 1193, 1827
BstBAI YACGTR 2 cut(s) 452, 558
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 7 cut(s) 310, 446, 636, 992, 1386, 1619, 1945
BstDEI CTNAG 3 cut(s) 153, 379, 1232
BstDSI CCRYGG 2 cut(s) 1160, 1170
BstFNI CGCG 9 cut(s) 72, 228, 461, 576, 591, 636, 717, 1162, 1604
BstH2I RGCGCY 5 cut(s) 315, 439, 1196, 1830, 1950
BstHHI GCGC 9 cut(s) 314, 438, 576, 593, 636, 638, 1195, 1829, 1949
BstMAI GTCTC 6 cut(s) 127, 156, 663, 1228, 1692, 1895
BstMCI CGRYCG 5 cut(s) 223, 724, 850, 1078, 1182
BstNI CCWGG 6 cut(s) 185, 424, 946, 1365, 1778, 1874
BstNSI RCATGY 1 cut(s) 1060
BstPAI GACNNNNGTC 1 cut(s) 803
BstSFI CTRYAG 1 cut(s) 326
BstSLI GKGCMC 1 cut(s) 1404
BstSNI TACGTA 1 cut(s) 558
BstUI CGCG 9 cut(s) 72, 228, 461, 576, 591, 636, 717, 1162, 1604
BstV1I GCAGC 7 cut(s) 312, 1269, 1272, 1329, 1627, 1633, 1946
BstV2I GAAGAC 6 cut(s) 881, 901, 922, 1026, 1863, 1884
BstX2I RGATCY 5 cut(s) 156, 204, 495, 564, 627
BstYI RGATCY 5 cut(s) 156, 204, 495, 564, 627
BstZI CGGCCG 2 cut(s) 847, 1075
BsuRI GGCC 8 cut(s) 290, 428, 608, 621, 849, 902, 1077, 1610
BtgI CCRYGG 2 cut(s) 1160, 1170
BtgZI GCGATG 2 cut(s) 442, 763
BtrI CACGTC 4 cut(s) 319, 600, 976, 1144
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 3 cut(s) 698, 1413, 2006
BveI ACCTGC 1 cut(s) 985
Cac8I GCNNGC 7 cut(s) 310, 446, 636, 992, 1386, 1619, 1945
CfoI GCGC 9 cut(s) 314, 438, 576, 593, 636, 638, 1195, 1829, 1949
Cfr10I RCCGGY 3 cut(s) 308, 1195, 1393
Cfr13I GGNCC 7 cut(s) 426, 852, 942, 1105, 1167, 1198, 1255
Cfr42I CCGCGG 1 cut(s) 1163
CseI GACGC 8 cut(s) 18, 61, 287, 746, 793, 1126, 1561, 1593
Csp6I GTAC 3 cut(s) 559, 1219, 1762
CspAI ACCGGT 1 cut(s) 1393
CviAII CATG 4 cut(s) 490, 820, 1057, 1171
CviQI GTAC 3 cut(s) 559, 1219, 1762
DdeI CTNAG 3 cut(s) 153, 379, 1232
DinI GGCGCC 4 cut(s) 313, 437, 1194, 1828
EaeI YGGCCR 5 cut(s) 288, 606, 847, 1075, 1608
EagI CGGCCG 2 cut(s) 847, 1075
Eam1104I CTCTTC 1 cut(s) 659
EarI CTCTTC 1 cut(s) 659
EciI GGCGGA 7 cut(s) 27, 199, 295, 409, 1058, 1600, 1829
Ecl136II GAGCTC 3 cut(s) 378, 516, 841
EclXI CGGCCG 2 cut(s) 847, 1075
Eco105I TACGTA 1 cut(s) 558
Eco130I CCWWGG 2 cut(s) 1170, 1484
Eco24I GRGCYC 4 cut(s) 380, 518, 843, 1484
Eco31I GGTCTC 4 cut(s) 663, 1228, 1692, 1895
Eco47I GGWCC 6 cut(s) 852, 942, 1105, 1167, 1198, 1255
Eco52I CGGCCG 2 cut(s) 847, 1075
Eco53kI GAGCTC 3 cut(s) 378, 516, 841
Eco57I CTGAAG 2 cut(s) 644, 916
Eco72I CACGTG 1 cut(s) 452
EcoICRI GAGCTC 3 cut(s) 378, 516, 841
EcoRII CCWGG 6 cut(s) 183, 422, 944, 1363, 1776, 1872
EcoT14I CCWWGG 2 cut(s) 1170, 1484
EcoT38I GRGCYC 4 cut(s) 380, 518, 843, 1484
EgeI GGCGCC 4 cut(s) 313, 437, 1194, 1828
EheI GGCGCC 4 cut(s) 313, 437, 1194, 1828
ErhI CCWWGG 2 cut(s) 1170, 1484
Esp3I CGTCTC 2 cut(s) 127, 156
FaeI CATG 4 cut(s) 493, 823, 1060, 1174
FaiI YATR 4 cut(s) 491, 821, 1058, 1172
FaqI GGGAC 4 cut(s) 218, 494, 1381, 1515
FatI CATG 4 cut(s) 489, 819, 1056, 1170
FauI CCCGC 6 cut(s) 143, 471, 685, 976, 1368, 1639
FblI GTMKAC 2 cut(s) 807, 1140
FriOI GRGCYC 4 cut(s) 380, 518, 843, 1484
FspBI CTAG 5 cut(s) 801, 1007, 1013, 1485, 1680
GlaI GCGC 9 cut(s) 313, 437, 575, 592, 635, 637, 1194, 1828, 1948
GsuI CTGGAG 1 cut(s) 1760
HaeII RGCGCY 5 cut(s) 315, 439, 1196, 1830, 1950
HaeIII GGCC 8 cut(s) 290, 428, 608, 621, 849, 902, 1077, 1610
HgaI GACGC 8 cut(s) 18, 61, 287, 746, 793, 1126, 1561, 1593
HhaI GCGC 9 cut(s) 314, 438, 576, 593, 636, 638, 1195, 1829, 1949
Hin1I GRCGYC 9 cut(s) 29, 231, 279, 312, 436, 738, 825, 1193, 1827
Hin1II CATG 4 cut(s) 493, 823, 1060, 1174
Hin6I GCGC 9 cut(s) 312, 436, 574, 591, 634, 636, 1193, 1827, 1947
HinP1I GCGC 9 cut(s) 312, 436, 574, 591, 634, 636, 1193, 1827, 1947
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HphI GGTGA 4 cut(s) 820, 1390, 1420, 1429
Hpy166II GTNNAC 3 cut(s) 76, 808, 1141
Hpy8I GTNNAC 3 cut(s) 76, 808, 1141
HpyAV CCTTC 6 cut(s) 751, 892, 1312, 1552, 1888, 1921
HpyCH4III ACNGT 4 cut(s) 1031, 1088, 1183, 1408
HpyCH4V TGCA 5 cut(s) 328, 355, 919, 1844, 1934
HpyF3I CTNAG 3 cut(s) 153, 379, 1232
Hsp92I GRCGYC 9 cut(s) 29, 231, 279, 312, 436, 738, 825, 1193, 1827
Hsp92II CATG 4 cut(s) 493, 823, 1060, 1174
HspAI GCGC 9 cut(s) 312, 436, 574, 591, 634, 636, 1193, 1827, 1947
KasI GGCGCC 4 cut(s) 311, 435, 1192, 1826
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
KspI CCGCGG 1 cut(s) 1163
Lsp1109I GCAGC 7 cut(s) 312, 1269, 1272, 1329, 1627, 1633, 1946
LweI GCATC 2 cut(s) 1845, 1989
MaeI CTAG 5 cut(s) 801, 1007, 1013, 1485, 1680
MaeIII GTNAC 5 cut(s) 696, 826, 1144, 1183, 1290
MbiI CCGCTC 2 cut(s) 417, 1459
MfeI CAATTG 1 cut(s) 856
MflI RGATCY 5 cut(s) 156, 204, 495, 564, 627
MhlI GDGCHC 8 cut(s) 219, 380, 518, 843, 1002, 1404, 1484, 1524
MluCI AATT 3 cut(s) 540, 856, 1702
Mly113I GGCGCC 4 cut(s) 312, 436, 1193, 1827
MlyI GAGTC 5 cut(s) 167, 176, 670, 779, 915
MmeI TCCRAC 3 cut(s) 195, 798, 868
MreI CGCCGGCG 1 cut(s) 308
MroNI GCCGGC 1 cut(s) 308
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 5 cut(s) 272, 1268, 1496, 1733, 2041
MslI CAYNNNNRTG 2 cut(s) 1472, 1807
MspA1I CMGCKG 1 cut(s) 1162
MunI CAATTG 1 cut(s) 856
Mva1269I GAATGC 2 cut(s) 480, 1633
MvaI CCWGG 6 cut(s) 185, 424, 946, 1365, 1778, 1874
MvnI CGCG 9 cut(s) 72, 228, 461, 576, 591, 636, 717, 1162, 1604
NaeI GCCGGC 1 cut(s) 310
NarI GGCGCC 4 cut(s) 312, 436, 1193, 1827
NciI CCSGG 6 cut(s) 340, 610, 754, 816, 1109, 1806
NcoI CCATGG 1 cut(s) 1170
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 4 cut(s) 493, 823, 1060, 1174
NlaIV GGNNCC 9 cut(s) 313, 347, 437, 584, 1194, 1401, 1659, 1665, 1828
NmeAIII GCCGAG 2 cut(s) 639, 862
NmuCI GTSAC 3 cut(s) 696, 826, 1144
NspI RCATGY 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 912
OliI CACNNNNGTG 1 cut(s) 1807
PaqCI CACCTGC 1 cut(s) 985
PauI GCGCGC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 9 cut(s) 29, 225, 516, 741, 1023, 1068, 1541, 1544, 1547
PctI GAATGC 2 cut(s) 480, 1633
PdiI GCCGGC 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 6 cut(s) 117, 786, 881, 1048, 1065, 1756
PflFI GACNNNGTC 2 cut(s) 769, 1906
PfoI TCCNGGA 1 cut(s) 944
PinAI ACCGGT 1 cut(s) 1393
PleI GAGTC 5 cut(s) 167, 175, 670, 778, 914
PluTI GGCGCC 4 cut(s) 315, 439, 1196, 1830
PmaCI CACGTG 1 cut(s) 452
PmlI CACGTG 1 cut(s) 452
PpsI GAGTC 5 cut(s) 167, 175, 670, 778, 914
Ppu21I YACGTR 2 cut(s) 452, 558
PshAI GACNNNNGTC 1 cut(s) 803
Psp124BI GAGCTC 3 cut(s) 380, 518, 843
Psp6I CCWGG 6 cut(s) 183, 422, 944, 1363, 1776, 1872
PspCI CACGTG 1 cut(s) 452
PspGI CCWGG 6 cut(s) 183, 422, 944, 1363, 1776, 1872
PspN4I GGNNCC 9 cut(s) 313, 347, 437, 584, 1194, 1401, 1659, 1665, 1828
PspPI GGNCC 7 cut(s) 426, 852, 942, 1105, 1167, 1198, 1255
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 5 cut(s) 156, 204, 495, 564, 627
PsyI GACNNNGTC 2 cut(s) 769, 1906
PteI GCGCGC 1 cut(s) 634
RsaI GTAC 3 cut(s) 560, 1220, 1763
RsaNI GTAC 3 cut(s) 559, 1219, 1762
RseI CAYNNNNRTG 2 cut(s) 1472, 1807
SacI GAGCTC 3 cut(s) 380, 518, 843
SacII CCGCGG 1 cut(s) 1163
SalI GTCGAC 1 cut(s) 1139
SaqAI TTAA 5 cut(s) 272, 1268, 1496, 1733, 2041
Sau96I GGNCC 7 cut(s) 426, 852, 942, 1105, 1167, 1198, 1255
SchI GAGTC 5 cut(s) 167, 176, 670, 779, 915
SduI GDGCHC 8 cut(s) 219, 380, 518, 843, 1002, 1404, 1484, 1524
SfaNI GCATC 2 cut(s) 1845, 1989
SfcI CTRYAG 1 cut(s) 326
SfoI GGCGCC 4 cut(s) 313, 437, 1194, 1828
Sfr303I CCGCGG 1 cut(s) 1163
SfuI TTCGAA 1 cut(s) 912
SgrAI CRCCGGYG 1 cut(s) 308
SgrBI CCGCGG 1 cut(s) 1163
SgrDI CGTCGACG 1 cut(s) 1139
SinI GGWCC 6 cut(s) 852, 942, 1105, 1167, 1198, 1255
SmiMI CAYNNNNRTG 2 cut(s) 1472, 1807
SmlI CTYRAG 2 cut(s) 44, 401
SmoI CTYRAG 2 cut(s) 44, 401
SnaBI TACGTA 1 cut(s) 558
Sse9I AATT 3 cut(s) 540, 856, 1702
SspDI GGCGCC 4 cut(s) 311, 435, 1192, 1826
SspMI CTAG 5 cut(s) 801, 1007, 1013, 1485, 1680
SstI GAGCTC 3 cut(s) 380, 518, 843
StyI CCWWGG 2 cut(s) 1170, 1484
TaaI ACNGT 4 cut(s) 1031, 1088, 1183, 1408
TaqI TCGA 8 cut(s) 219, 510, 615, 735, 744, 912, 1140, 1478
TaqII GACCGA 2 cut(s) 782, 893
TasI AATT 3 cut(s) 540, 856, 1702
TfiI GAWTC 6 cut(s) 117, 786, 881, 1048, 1065, 1756
Tru1I TTAA 5 cut(s) 272, 1268, 1496, 1733, 2041
Tru9I TTAA 5 cut(s) 272, 1268, 1496, 1733, 2041
TscAI CASTG 3 cut(s) 705, 1413, 2006
TseFI GTSAC 3 cut(s) 696, 826, 1144
TseI GCWGC 7 cut(s) 325, 1282, 1285, 1342, 1621, 1640, 1934
Tsp45I GTSAC 3 cut(s) 696, 826, 1144
TspDTI ATGAA 2 cut(s) 1735, 1869
TspRI CASTG 3 cut(s) 705, 1413, 2006
Tth111I GACNNNGTC 2 cut(s) 769, 1906
VpaK11BI GGWCC 6 cut(s) 852, 942, 1105, 1167, 1198, 1255
XbaI TCTAGA 1 cut(s) 1006
XceI RCATGY 1 cut(s) 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 1411
XmaJI CCTAGG 1 cut(s) 1484
XmiI GTMKAC 2 cut(s) 807, 1140
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 5 cut(s) 801, 1007, 1013, 1485, 1680
ZraI GACGTC 2 cut(s) 232, 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.