Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005786

Physical Location & Seq

Genomic Coordinates Forward (+)

46870569 .. 46876001

Gene Structure 5433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1351900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGCTGTTCACCCTTTTGCTAACTCGCTCTCTGTGACTCGCTGGCTCATTGAGTTCGTGCTAATCGCCTCGGCTTTGGCTGCTCTGCCTCTCGTTAGTGGCACCACTGATGCTTCTGATGTTCAAGCACTTCAAGTTTTGTACACCTCAGTAAACAGTCCTTCTCAGCTAACCGGTTGGACAAGTAGTGGCGGTGATCCATGTGGAGAGTCATGGAAAGGGGTAACTTGTGAGGGCTCAGCTGTTGTTGCAGTAGAGGTTTCTGGGTTAGGTCTCAGTGGAACTATGGGATACTTGCTTTCCGATCTGCTATCGTTGAGAAAATTTGATTTGAGTGACAACAATTTTCATGATACAATCCCGTATCAACTACCTCCAAATCTTACAAGCCTAAATCTTGCAAGGAACAACTTCACGGGAAATTTACCTTATTCTATTGCCAACATGGTTTCTCTCAGCTATTTGAATGTTAGCCGTAACTCACTATCCCAGTCGATTGGTGACATTTTTGCTCATCTTGTGAGCCTTGCAACCTTGGATCTATCTCTTAACAACTTCACCAGTGATATTCCTAGTTCCTTAAGTTCATTATCCAATCTTTCTTCCCTCTATGTGCAGAATAATCAGTTGACTGGTTCTCTCAATGTTTTGACGGGTTTGCCCTTGACTACTTTAAATGTTGCGAACAACCATTTCAGCGGGTGGATACCTAGAGAGCTTAGTTCAATTCGTACTTTTATTTATGATGGAAATGGTTTTGATAATGGTCCTGCTCCTCCGCCACCACCATATATCCCACCTCCCCCAGGAAGAACTACTAAGAATCGCAGCCATTCTGGATCTGGAACACCCCCACGGGGATCTGATGGCCAATCACATGATTCAAAAAAAGGGCTGGCTGTCGGGGCTTTGGTAGGGATAATTCTGGGTTCTGTTGTTGTTGCTCTCATTGTCTTACTTGCTCTTGCTTTTTGCATTCGCAAAAACAAAAGGGAGGACAGGACTCCAAGAGCTTCTGGTGGAAATGTCTCTGCTCGCACAAATAATGTAAATACTGAGATGCAAGAGCAGAGGGTAAAAAGTACGGCTGCTGTAACTGATATCAAGCCCCCACCCGCAGAAATATTGGTGGTTGATAGGTTACATAGTAAAAATGGATCGACAAAAAGGATAAAATCACCTATCACTGCTACTCCTTATACTGTTGCCTCTCTCCAAACAGCAACAAATAGCTTTAGTCAAGAATTTCTTATTGGTGAAGGTTCTCTTGGTCGTGTTTACAGAGCAGAGTTCCCTAATGGAAAGGTAATGGCCATTAAGAAAATTGACAATGCAGCACTATCCTTGCAAGAGGAAGATAATTTCCTTGAAGCTATTTCAAATATGTCGCGCTTGAGGCATCCAAACATTGTTACATTGGCTGGATATTGTGCAGAGCATGGACAGCGTCTTCTAGTCTATGAGTATGTAGGAAATGGGAGCTTGCATGACATTCTTCATTTTGCTGAAGACGGCAGTAATACTCTGACTTGGAATGCTCGTGTTAGGGTAGCACTTGGCACTGCTCGAGCTTTAGAGTACTTGCATGAAGTTTGCTTACCTTCTGTTGTACATAGAAACTTCAAGTCAGCAAATATTTTACTTGATGAAGAGCTTAATCCCCACTTATCTGACTGTGGGTTAGCTGCTCTAACGCCAAACACAGAGCGACAGGTTTCAACTCAGATGGTAGGTTCATTTGGTTATAGTGCTCCCGAATTTGCCTTATCAGGAGTATACACTGTAAAAAGTGACGTCTACAGCTTTGGGGTGGTTATGCTCGAGCTGTTGACTGGTCGGAAGCCACTAGACAGTTCAAGGTCAAGGTCAGAACAATCACTTGTGAGATGGGCTACTCCCCAACTACATGATATAGATGCCCTATCAAAAATGGTTGATCCGGCTTTAAATGGCATGTATCCGGCAAAGTCACTTTCACGTTTCGCTGACATCATTGCACTTTGTGTTCAGCCGGAACCCGAATTTCGGCCTCCCATGTCCGAAGTTGTGCAAGCACTGGTCCGCTTAGTACAAAGAGCAAGCGTGGTCAGACGTTCAAGTGACGAATCTAGTTTCGGATTCAAGACCCCCGATCATGAAGCAATTGACATGTCGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

720

Amino Acids

77.61

Weight (kDa)

6.36

Isoelectric Point (pI)

36.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	38 - 77	5e-11	Leucine rich repeat N-terminal domain
LRR_8	PF13855	126 - 186	1.9e-07	Leucine rich repeat
CR_prot_dom_plant	PF22812	257 - 330	9e-06	Plant cysteine-rich protein domain
Pkinase	PF00069	415 - 684	4.4e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	417 - 686	1.9e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22130 AT4G22130
fragaria_vesca	FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460
malus_domestica	MD05G1351900.v1.1 MD10G1326000.v1.1
prunus_persica	Prupe.4G015700_v2.0.a1 Prupe.4G015700_v2.0.a1 Prupe.4G015700_v2.0.a1
pyrus_communis	pycom05g31890 pycom10g27590 pycom10g27600
rosa_chinensis	RchiOBHm_Chr5g0002301
rosa_laevigata	RLG00000031016
rosa_multiflora	Rmu_sc0001492.1_g000010
rosa_roxburghii	Rroxscaffold_1G00073570 Rroxscaffold_1G00073600
rosa_rugosa	Rorug04G0395200
rosa_samantha	Rh5AG017900 Rh5BG020500 Rh5CG019700 Rh5DG018600
rosa_wichuraiana	Rw5G001680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1797
AccB1I	GGYRCC	1 cut(s)	101
AccI	GTMKAC	2 cut(s)	1776, 1797
AccII	CGCG	1 cut(s)	1390
AciI	CCGC	5 cut(s)	192, 699, 779, 1116, 2062
AclWI	GGATC	6 cut(s)	191, 546, 847, 868, 1165, 1931
AcoI	YGGCCR	2 cut(s)	868, 1311
AcsI	RAATTY	5 cut(s)	323, 421, 1244, 1757, 2021
AcuI	CTGAAG	1 cut(s)	1527
AcyI	GRCGYC	1 cut(s)	1794
AdeI	CACNNNGTG	1 cut(s)	2003
AfaI	GTAC	6 cut(s)	143, 733, 1084, 1580, 1611, 2070
AfiI	CCNNNNNNNGG	3 cut(s)	806, 857, 2025
AflII	CTTAAG	1 cut(s)	580
AflIII	ACRYGT	1 cut(s)	2148
AgeI	ACCGGT	1 cut(s)	173
AjnI	CCWGG	1 cut(s)	805
AjuI	GAANNNNNNNTTGG	4 cut(s)	1236, 1251, 1268, 1283
Alw21I	GWGCWC	1 cut(s)	1753
Alw26I	GTCTC	2 cut(s)	278, 1033
AlwI	GGATC	6 cut(s)	191, 546, 847, 868, 1165, 1931
Ama87I	CYCGRG	2 cut(s)	1566, 1820
AoxI	GGCC	3 cut(s)	868, 1311, 2027
ApeKI	GCWGC	5 cut(s)	80, 828, 1088, 1334, 1685
ApoI	RAATTY	5 cut(s)	323, 421, 1244, 1757, 2021
AsiGI	ACCGGT	1 cut(s)	173
Asp700I	GAANNNNTTC	1 cut(s)	410
AspLEI	GCGC	1 cut(s)	1392
AspS9I	GGNCC	2 cut(s)	767, 2059
AsuHPI	GGTGA	5 cut(s)	206, 512, 550, 1170, 1268
AvaI	CYCGRG	2 cut(s)	1566, 1820
AvaII	GGWCC	2 cut(s)	767, 2059
BalI	TGGCCA	2 cut(s)	870, 1313
BanI	GGYRCC	1 cut(s)	101
BanII	GRGCYC	1 cut(s)	239
BarI	GAAGNNNNNNTAC	2 cut(s)	1581, 1613
BauI	CACGAG	1 cut(s)	1539
BbsI	GAAGAC	2 cut(s)	1442, 1515
Bbv12I	GWGCWC	1 cut(s)	1753
BbvI	GCAGC	5 cut(s)	67, 840, 1075, 1346, 1672
BccI	CCATC	4 cut(s)	740, 860, 1720, 1881
BceAI	ACGGC	3 cut(s)	459, 1101, 1528
BciT130I	CCWGG	1 cut(s)	807
BciVI	GTATCC	3 cut(s)	284, 699, 1968
BcoDI	GTCTC	2 cut(s)	278, 1033
BfaI	CTAG	5 cut(s)	573, 711, 1454, 1847, 2109
BfmI	CTRYAG	1 cut(s)	1798
BfrI	CTTAAG	1 cut(s)	580
BfuI	GTATCC	3 cut(s)	284, 699, 1968
BisI	GCNGC	5 cut(s)	81, 829, 1089, 1335, 1686
BlpI	GCTNAGC	1 cut(s)	238
BlsI	GCNGC	5 cut(s)	82, 830, 1090, 1336, 1687
BmcAI	AGTACT	1 cut(s)	1580
Bme1390I	CCNGG	1 cut(s)	807
Bme18I	GGWCC	2 cut(s)	767, 2059
BmeT110I	CYCGRG	2 cut(s)	1566, 1820
BmgT120I	GGNCC	2 cut(s)	767, 2059
BmiI	GGNNCC	2 cut(s)	103, 2016
BmrFI	CCNGG	1 cut(s)	807
BmrI	ACTGGG	1 cut(s)	484
BmsI	GCATC	4 cut(s)	100, 1050, 1408, 1906
BmuI	ACTGGG	1 cut(s)	484
BpiI	GAAGAC	2 cut(s)	1442, 1515
Bpu1102I	GCTNAGC	1 cut(s)	238
BpuEI	CTTGAG	1 cut(s)	1414
BsaHI	GRCGYC	1 cut(s)	1794
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	4 cut(s)	69, 534, 805, 854
BsaWI	WCCGGW	1 cut(s)	173
BsaXI	ACNNNNNCTCC	2 cut(s)	1177, 1207
Bsc4I	CCNNNNNNNGG	3 cut(s)	806, 857, 2025
Bse118I	RCCGGY	1 cut(s)	173
Bse1I	ACTGG	5 cut(s)	490, 561, 637, 1837, 2061
Bse3DI	GCAATG	1 cut(s)	1992
BseBI	CCWGG	1 cut(s)	807
BseDI	CCNNGG	4 cut(s)	69, 534, 805, 854
BseGI	GGATG	1 cut(s)	1399
BseLI	CCNNNNNNNGG	3 cut(s)	806, 857, 2025
BseMI	GCAATG	1 cut(s)	1992
BseMII	CTCAG	7 cut(s)	162, 179, 252, 289, 469, 1047, 1736
BseNI	ACTGG	5 cut(s)	490, 561, 637, 1837, 2061
BseRI	GAGGAG	1 cut(s)	765
BseXI	GCAGC	5 cut(s)	67, 840, 1075, 1346, 1672
BsgI	GTGCAG	2 cut(s)	635, 1452
Bsh1236I	CGCG	1 cut(s)	1390
BshFI	GGCC	3 cut(s)	870, 1313, 2029
BshNI	GGYRCC	1 cut(s)	101
BshTI	ACCGGT	1 cut(s)	173
BsiHKAI	GWGCWC	1 cut(s)	1753
BsiHKCI	CYCGRG	2 cut(s)	1566, 1820
BsiSI	CCGG	4 cut(s)	174, 1940, 1961, 2012
BslI	CCNNNNNNNGG	3 cut(s)	806, 857, 2025
BsmAI	GTCTC	2 cut(s)	278, 1033
BsmI	GAATGC	2 cut(s)	975, 1540
BsnI	GGCC	3 cut(s)	870, 1313, 2029
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	2 cut(s)	1566, 1820
Bsp1286I	GDGCHC	2 cut(s)	239, 1753
Bsp1407I	TGTACA	2 cut(s)	141, 1609
Bsp143I	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
Bsp1720I	GCTNAGC	1 cut(s)	238
BspACI	CCGC	5 cut(s)	192, 699, 779, 1116, 2062
BspANI	GGCC	3 cut(s)	870, 1313, 2029
BspCNI	CTCAG	7 cut(s)	161, 178, 251, 288, 468, 1048, 1735
BspFNI	CGCG	1 cut(s)	1390
BspHI	TCATGA	2 cut(s)	349, 2134
BspLI	GGNNCC	2 cut(s)	103, 2016
BspPI	GGATC	6 cut(s)	191, 546, 847, 868, 1165, 1931
BspQI	GCTCTTC	1 cut(s)	1644
BspT107I	GGYRCC	1 cut(s)	101
BspTI	CTTAAG	1 cut(s)	580
BspTNI	GGTCTC	1 cut(s)	278
BsrDI	GCAATG	1 cut(s)	1992
BsrFI	RCCGGY	1 cut(s)	173
BsrGI	TGTACA	2 cut(s)	141, 1609
BsrI	ACTGG	5 cut(s)	490, 561, 637, 1837, 2061
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	4 cut(s)	69, 534, 805, 854
BssMI	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
BssNAI	GTATAC	1 cut(s)	1777
BssNI	GRCGYC	1 cut(s)	1794
BssSI	CACGAG	1 cut(s)	1539
BssT1I	CCWWGG	1 cut(s)	534
Bst1107I	GTATAC	1 cut(s)	1777
Bst2BI	CACGAG	1 cut(s)	1539
Bst2UI	CCWGG	1 cut(s)	807
Bst4CI	ACNGT	5 cut(s)	158, 1204, 1676, 1783, 1853
Bst6I	CTCTTC	1 cut(s)	1644
BstACI	GRCGYC	1 cut(s)	1794
BstAFI	CTTAAG	1 cut(s)	580
BstAUI	TGTACA	2 cut(s)	141, 1609
BstC8I	GCNNGC	6 cut(s)	44, 897, 1036, 1484, 2052, 2080
BstDSI	CCRYGG	1 cut(s)	854
BstENI	CCTNNNNNAGG	1 cut(s)	804
BstF5I	GGATG	1 cut(s)	1399
BstFNI	CGCG	1 cut(s)	1390
BstHHI	GCGC	1 cut(s)	1392
BstKTI	GATC	8 cut(s)	199, 307, 541, 842, 863, 1160, 1939, 2134
BstMAI	GTCTC	2 cut(s)	278, 1033
BstMBI	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 248, 905, 1396, 1444
BstNI	CCWGG	1 cut(s)	807
BstNSI	RCATGY	2 cut(s)	1957, 2152
BstSCI	CCNGG	1 cut(s)	805
BstSFI	CTRYAG	1 cut(s)	1798
BstUI	CGCG	1 cut(s)	1390
BstV1I	GCAGC	5 cut(s)	67, 840, 1075, 1346, 1672
BstV2I	GAAGAC	2 cut(s)	1442, 1515
BstX2I	RGATCY	3 cut(s)	538, 839, 860
BstXI	CCANNNNNNTGG	1 cut(s)	497
BstYI	RGATCY	3 cut(s)	538, 839, 860
BstZ17I	GTATAC	1 cut(s)	1777
BsuI	GTATCC	3 cut(s)	284, 699, 1968
BsuRI	GGCC	3 cut(s)	870, 1313, 2029
BtgI	CCRYGG	1 cut(s)	854
BtsCI	GGATG	1 cut(s)	1399
BtsI	GCAGTG	2 cut(s)	1185, 1560
BtsIMutI	CAGTG	7 cut(s)	105, 283, 568, 1185, 1560, 1779, 2054
Cac8I	GCNNGC	6 cut(s)	44, 897, 1036, 1484, 2052, 2080
CciI	TCATGA	2 cut(s)	349, 2134
CfoI	GCGC	1 cut(s)	1392
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	2 cut(s)	767, 2059
CseI	GACGC	1 cut(s)	1436
Csp6I	GTAC	6 cut(s)	142, 732, 1083, 1579, 1610, 2069
CspAI	ACCGGT	1 cut(s)	173
CviQI	GTAC	6 cut(s)	142, 732, 1083, 1579, 1610, 2069
DpnI	GATC	8 cut(s)	198, 306, 540, 841, 862, 1159, 1938, 2133
DpnII	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
DraI	TTTAAA	2 cut(s)	675, 1947
DraIII	CACNNNGTG	1 cut(s)	2003
EaeI	YGGCCR	2 cut(s)	868, 1311
Eam1104I	CTCTTC	1 cut(s)	1644
EarI	CTCTTC	1 cut(s)	1644
EciI	GGCGGA	1 cut(s)	768
Eco130I	CCWWGG	1 cut(s)	534
Eco24I	GRGCYC	1 cut(s)	239
Eco31I	GGTCTC	1 cut(s)	278
Eco32I	GATATC	1 cut(s)	1102
Eco47I	GGWCC	2 cut(s)	767, 2059
Eco57I	CTGAAG	1 cut(s)	1527
Eco88I	CYCGRG	2 cut(s)	1566, 1820
EcoNI	CCTNNNNNAGG	1 cut(s)	804
EcoRII	CCWGG	1 cut(s)	805
EcoRV	GATATC	1 cut(s)	1102
EcoT14I	CCWWGG	1 cut(s)	534
EcoT38I	GRGCYC	1 cut(s)	239
ErhI	CCWWGG	1 cut(s)	534
FalI	AAGNNNNNCTT	4 cut(s)	1233, 1265, 1251, 1283
FauI	CCCGC	2 cut(s)	692, 1123
FblI	GTMKAC	2 cut(s)	1776, 1797
Fnu4HI	GCNGC	5 cut(s)	81, 829, 1089, 1335, 1686
FokI	GGATG	1 cut(s)	1386
FriOI	GRGCYC	1 cut(s)	239
Fsp4HI	GCNGC	5 cut(s)	81, 829, 1089, 1335, 1686
FspBI	CTAG	5 cut(s)	573, 711, 1454, 1847, 2109
GlaI	GCGC	1 cut(s)	1391
GluI	GCNGC	5 cut(s)	81, 829, 1089, 1335, 1686
HaeIII	GGCC	3 cut(s)	870, 1313, 2029
HapII	CCGG	4 cut(s)	174, 1940, 1961, 2012
HgaI	GACGC	1 cut(s)	1436
HhaI	GCGC	1 cut(s)	1392
Hin1I	GRCGYC	1 cut(s)	1794
Hin6I	GCGC	1 cut(s)	1390
HinP1I	GCGC	1 cut(s)	1390
HincII	GTYRAC	2 cut(s)	630, 1830
HindII	GTYRAC	2 cut(s)	630, 1830
HinfI	GANTC	7 cut(s)	37, 209, 823, 881, 1003, 2105, 2118
HpaII	CCGG	4 cut(s)	174, 1940, 1961, 2012
HphI	GGTGA	5 cut(s)	206, 512, 550, 1170, 1268
Hpy166II	GTNNAC	8 cut(s)	10, 144, 154, 630, 1279, 1777, 1798, 1830
Hpy188III	TCNNGA	8 cut(s)	350, 837, 843, 1241, 1754, 1770, 2122, 2135
Hpy8I	GTNNAC	8 cut(s)	10, 144, 154, 630, 1279, 1777, 1798, 1830
HpyAV	CCTTC	3 cut(s)	171, 1253, 1611
HpyCH4III	ACNGT	5 cut(s)	158, 1204, 1676, 1783, 1853
HpyCH4IV	ACGT	3 cut(s)	1794, 1978, 2092
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 248, 905, 1396, 1444
HpySE526I	ACGT	3 cut(s)	1794, 1978, 2092
Hsp92I	GRCGYC	1 cut(s)	1794
HspAI	GCGC	1 cut(s)	1390
Kzo9I	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
LguI	GCTCTTC	1 cut(s)	1644
LmnI	GCTCC	3 cut(s)	778, 1479, 1756
Lsp1109I	GCAGC	5 cut(s)	67, 840, 1075, 1346, 1672
LweI	GCATC	4 cut(s)	100, 1050, 1408, 1906
MaeI	CTAG	5 cut(s)	573, 711, 1454, 1847, 2109
MaeII	ACGT	3 cut(s)	1794, 1978, 2092
MalI	GATC	8 cut(s)	198, 306, 540, 841, 862, 1159, 1938, 2133
MboI	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
MboII	GAAGA	7 cut(s)	594, 822, 1367, 1442, 1487, 1520, 1661
MfeI	CAATTG	1 cut(s)	2142
MflI	RGATCY	3 cut(s)	538, 839, 860
MhlI	GDGCHC	2 cut(s)	239, 1753
MlsI	TGGCCA	2 cut(s)	870, 1313
MluNI	TGGCCA	2 cut(s)	870, 1313
MlyI	GAGTC	3 cut(s)	31, 218, 997
MmeI	TCCRAC	2 cut(s)	158, 1817
Mox20I	TGGCCA	2 cut(s)	870, 1313
MroXI	GAANNNNTTC	1 cut(s)	410
MscI	TGGCCA	2 cut(s)	870, 1313
MseI	TTAA	7 cut(s)	549, 581, 674, 1317, 1656, 1946, 2158
Msp20I	TGGCCA	2 cut(s)	870, 1313
MspA1I	CMGCKG	2 cut(s)	242, 699
MspCI	CTTAAG	1 cut(s)	580
MspI	CCGG	4 cut(s)	174, 1940, 1961, 2012
MspR9I	CCNGG	1 cut(s)	807
MunI	CAATTG	1 cut(s)	2142
Mva1269I	GAATGC	2 cut(s)	975, 1540
MvaI	CCWGG	1 cut(s)	807
MvnI	CGCG	1 cut(s)	1390
MwoI	GCNNNNNNNGC	5 cut(s)	80, 248, 905, 1396, 1444
NdeII	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
NlaIV	GGNNCC	2 cut(s)	103, 2016
NmeAIII	GCCGAG	1 cut(s)	50
NmuCI	GTSAC	6 cut(s)	34, 335, 500, 1790, 1968, 2099
NspI	RCATGY	2 cut(s)	1957, 2152
PaeR7I	CTCGAG	2 cut(s)	1566, 1820
PagI	TCATGA	2 cut(s)	349, 2134
PciI	ACATGT	1 cut(s)	2148
PciSI	GCTCTTC	1 cut(s)	1644
PctI	GAATGC	2 cut(s)	975, 1540
PdmI	GAANNNNTTC	1 cut(s)	410
PfeI	GAWTC	4 cut(s)	823, 881, 2105, 2118
PflFI	GACNNNGTC	1 cut(s)	1446
PinAI	ACCGGT	1 cut(s)	173
PkrI	GCNGC	5 cut(s)	82, 830, 1090, 1336, 1687
PleI	GAGTC	3 cut(s)	31, 217, 997
PpsI	GAGTC	3 cut(s)	31, 217, 997
PscI	ACATGT	1 cut(s)	2148
Psp6I	CCWGG	1 cut(s)	805
PspGI	CCWGG	1 cut(s)	805
PspN4I	GGNNCC	2 cut(s)	103, 2016
PspPI	GGNCC	2 cut(s)	767, 2059
PspXI	VCTCGAGB	2 cut(s)	1566, 1820
PsuI	RGATCY	3 cut(s)	538, 839, 860
PsyI	GACNNNGTC	1 cut(s)	1446
PvuII	CAGCTG	1 cut(s)	242
RsaI	GTAC	6 cut(s)	143, 733, 1084, 1580, 1611, 2070
RsaNI	GTAC	6 cut(s)	142, 732, 1083, 1579, 1610, 2069
SapI	GCTCTTC	1 cut(s)	1644
SaqAI	TTAA	7 cut(s)	549, 581, 674, 1317, 1656, 1946, 2158
SatI	GCNGC	5 cut(s)	81, 829, 1089, 1335, 1686
Sau3AI	GATC	8 cut(s)	196, 304, 538, 839, 860, 1157, 1936, 2131
Sau96I	GGNCC	2 cut(s)	767, 2059
ScaI	AGTACT	1 cut(s)	1580
SchI	GAGTC	3 cut(s)	31, 218, 997
ScrFI	CCNGG	1 cut(s)	807
SduI	GDGCHC	2 cut(s)	239, 1753
SfaNI	GCATC	4 cut(s)	100, 1050, 1408, 1906
SfcI	CTRYAG	1 cut(s)	1798
Sfr274I	CTCGAG	2 cut(s)	1566, 1820
SinI	GGWCC	2 cut(s)	767, 2059
SlaI	CTCGAG	2 cut(s)	1566, 1820
SmlI	CTYRAG	4 cut(s)	580, 1393, 1566, 1820
SmoI	CTYRAG	4 cut(s)	580, 1393, 1566, 1820
SsiI	CCGC	5 cut(s)	192, 699, 779, 1116, 2062
SspI	AATATT	2 cut(s)	1125, 1636
SspMI	CTAG	5 cut(s)	573, 711, 1454, 1847, 2109
StyD4I	CCNGG	1 cut(s)	805
StyI	CCWWGG	1 cut(s)	534
TaaI	ACNGT	5 cut(s)	158, 1204, 1676, 1783, 1853
TaiI	ACGT	3 cut(s)	1797, 1981, 2095
TaqI	TCGA	4 cut(s)	494, 1160, 1567, 1821
TatI	WGTACW	4 cut(s)	141, 1578, 1609, 2068
TfiI	GAWTC	4 cut(s)	823, 881, 2105, 2118
Tru1I	TTAA	7 cut(s)	549, 581, 674, 1317, 1656, 1946, 2158
Tru9I	TTAA	7 cut(s)	549, 581, 674, 1317, 1656, 1946, 2158
TscAI	CASTG	7 cut(s)	112, 283, 568, 1192, 1567, 1786, 2061
TseFI	GTSAC	6 cut(s)	34, 335, 500, 1790, 1968, 2099
TseI	GCWGC	5 cut(s)	80, 828, 1088, 1334, 1685
Tsp45I	GTSAC	6 cut(s)	34, 335, 500, 1790, 1968, 2099
TspDTI	ATGAA	7 cut(s)	338, 576, 1487, 1602, 1662, 1725, 2151
TspRI	CASTG	7 cut(s)	112, 283, 568, 1192, 1567, 1786, 2061
Tth111I	GACNNNGTC	1 cut(s)	1446
Vha464I	CTTAAG	1 cut(s)	580
VpaK11BI	GGWCC	2 cut(s)	767, 2059
XagI	CCTNNNNNAGG	1 cut(s)	804
XapI	RAATTY	5 cut(s)	323, 421, 1244, 1757, 2021
XceI	RCATGY	2 cut(s)	1957, 2152
XcmI	CCANNNNNNNNNTGG	1 cut(s)	839
XhoI	CTCGAG	2 cut(s)	1566, 1820
XmiI	GTMKAC	2 cut(s)	1776, 1797
XmnI	GAANNNNTTC	1 cut(s)	410
XspI	CTAG	5 cut(s)	573, 711, 1454, 1847, 2109
ZraI	GACGTC	1 cut(s)	1795
ZrmI	AGTACT	1 cut(s)	1580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.