Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005786

Physical Location & Seq

Genomic Coordinates Forward (+)

40635329 .. 40640989

Gene Structure 5661 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1326000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGGCTGATCACCCTTCTGCTGACTCGCTCTCTGTGACTCGCTGGCTCACTGAGTTCGTGCTAATCGCCTCGACTTTGGCTGCTCTGCCTCTCGTTAGTGGCACCACCGATGCTTCTGATGTTCGAGCACTTCAGGTACTGTACACCTCAGTAAACAGTCCTTCTCAGCTAACCGGTTGGAAAAGTAGTGGCGGTGATCCGTGTGGAGAGTCATGGAAAGGGGTAACTTGTGAGGGCTCAGCTGTTGTTTCAGTAGAGGTTTCCGGGTTAGGTCTCAGTGGAACTATTGGATACTTGCTTTCCGACCTGCTATCATTGTTAAAACTAAATCTTGCAAGGAACAACTTCACTGGAAATTTACCTTATTCTATTGCCAACATGGTTTCTCTCAGTTATTTGAATGTTAGCCGTAACTCACTATCCCAGTCAATTGGTGACATTTTTGCTCATCTTGCTGGCCTTTCTACCTTGGATCTATCTCTTAACAACTTTACCAGTGATATTCCTAATTCCTTAAGTTCATTGTCCAATCTTTCTTCCCTCTATATGCAGAACAATCAACTGACTGGTACTCTCAATGTTCTGACGGGTTTGCCTTTGACTACTTTAAATGTTGCCAACAACCATTTCAGTGGATGGATACCCCAAGAGCTTAGTTCAATTCGTACTTTTATTTATGATGGAAATGGTTTTGATAATGGTCCTGCTCCTCCACCACCACCATATAACCCACCTCCCCCGGGAAGATCTACTAAGAATCGCAGTCGTTCCGGATCTGGAACACCCCCACAGGGATCTGATGGCCAATCACATGATTCAAAAAATGGGCTGGCCGTTGGGGCTTTGGTAGGGATAATTCTGGGTTCTGTTTTTGTTGCTCTCATTGTCTTACTTGCTCTTGCCTTTTGCATACGCAAAAATAAAAGGGAGGACAGGATTCCAAGAGCTTCTGGGGGAAATGTCTCTGCTCGCACAAATAATGTAAATACTGAGATGCAAGAGCAGAGGGTAAAAAGTGCGGCTGCTGTAACTGATCTCATGCCTCCACCCGCAGAAATTTTGGTGGTTGATAGGTTACATAGTAAAAATGGATCGATAAAAAGGATAAAATCACCTATCACTGCTACTCCTTATACTGTAGCCTCTCTCCAATCAGCAACTAATAGCTTCAGTCAAGAATTTCTTATTGGTGAAGGTTCTCTTGGACGTGTTTACAGAGCAGAGTTCCCTAATGGAAAGATAATGGCAATTAAGAAAATTGACAATGCAGCACTATCATTGCAAGAGGAAGATAATTTCCTTGAAGCTATTTCAAATATGTCGCGCTTGAGGCATCAAAACATTGTTACACTGGCTGGATATTGCGCAGAGCATGGGCAGTGTCTTCTAGTCTATGAGCATATAGGAAACGGGAGCTTGCATGACATTCTTCATTTTGCTGAAGATGGCAGTAAGACTCTGAATTGGAATGCCCGTGTTAGGGTAGCACTTGGCACAGCTCGAGCTTTAGAGTACTTGCATGAAGTTTGCTTACCTTCTGTTGTACATAGAAACTTCAAGTCAGCAAATATTTTACTTGATGAAGAGCTCAATCCCCACTTATCTGACTGTGGATTAGCTGCTCTAACGCCAAACACAGAGCGACAGGTTTCACCTCAGATGGTGGGTTCATTTGGTTATAGTGCTCCTGAATTCGCCTTGTCAGGAGTATACACTGTAAAAAGTGACGTCTACAGCTTTGGGGTGGTGATGCTCGAGCTGTTGACTGGTCGGAAGCCACTAGACAGTTCAAGGTCGAGGTCAGAACAGTCACTTGTGAGATGGGCTACTCCCCAACTACATGATATAGACGCCCTAGCAAAAATGGTTGATCCGGCCTTAAATGGCATGTATCCTGCGAAGTCTCTTTCACGTTTCGCTGACGTCATTGCGCTTTGTGTTCAGCCGGAGCCTGAATTCCGGCCTCCCATGTCCGAAGTTGTGCAAGCGCTGGTCCGCTTAGTGCAAAGAGCAAGCGTGGTCAGACGTTCAAGTGACGAATCTGGTTTCGCATACAGGACCCCCGATCACGAAGCAATTGACATGCCATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

698

Amino Acids

75.08

Weight (kDa)

6.22

Isoelectric Point (pI)

41.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	38 - 77	1.6e-10	Leucine rich repeat N-terminal domain
LRR_8	PF13855	146 - 188	2.5e-06	Leucine rich repeat
CR_prot_dom_plant	PF22812	235 - 308	8.6e-06	Plant cysteine-rich protein domain
Pkinase	PF00069	393 - 662	5.3e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	395 - 664	7.3e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22130 AT4G22130
fragaria_vesca	FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460 FvH4_3g01460
malus_domestica	MD05G1351900.v1.1 MD10G1326000.v1.1
prunus_persica	Prupe.4G015700_v2.0.a1 Prupe.4G015700_v2.0.a1 Prupe.4G015700_v2.0.a1
pyrus_communis	pycom05g31890 pycom10g27590 pycom10g27600
rosa_chinensis	RchiOBHm_Chr5g0002301
rosa_laevigata	RLG00000031016
rosa_multiflora	Rmu_sc0001492.1_g000010
rosa_roxburghii	Rroxscaffold_1G00073570 Rroxscaffold_1G00073600
rosa_rugosa	Rorug04G0395200
rosa_samantha	Rh5AG017900 Rh5BG020500 Rh5CG019700 Rh5DG018600
rosa_wichuraiana	Rw5G001680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1731, 1926
Acc16I	TGCGCA	1 cut(s)	1366
Acc36I	ACCTGC	1 cut(s)	315
AccB1I	GGYRCC	1 cut(s)	101
AccI	GTMKAC	2 cut(s)	1710, 1731
AccII	CGCG	1 cut(s)	1324
AccIII	TCCGGA	1 cut(s)	770
AciI	CCGC	4 cut(s)	192, 1019, 1050, 1996
AclWI	GGATC	6 cut(s)	191, 480, 781, 802, 1099, 1865
AcoI	YGGCCR	2 cut(s)	802, 831
AcsI	RAATTY	5 cut(s)	355, 1056, 1178, 1691, 1955
AcuI	CTGAAG	3 cut(s)	116, 1153, 1461
AcyI	GRCGYC	3 cut(s)	1728, 1851, 1923
AfaI	GTAC	6 cut(s)	138, 143, 571, 667, 1514, 1545
AfeI	AGCGCT	1 cut(s)	1989
AfiI	CCNNNNNNNGG	4 cut(s)	740, 791, 1479, 1480
AflII	CTTAAG	1 cut(s)	514
AflIII	ACRYGT	1 cut(s)	1207
AgeI	ACCGGT	1 cut(s)	173
AgsI	TTSAA	8 cut(s)	400, 660, 819, 1304, 1314, 1558, 1791, 2031
AjiI	CACGTC	1 cut(s)	1208
AjuI	GAANNNNNNNTTGG	6 cut(s)	611, 643, 1170, 1185, 1202, 1217
Alw21I	GWGCWC	3 cut(s)	130, 1590, 1687
Alw26I	GTCTC	3 cut(s)	278, 967, 1908
AlwI	GGATC	6 cut(s)	191, 480, 781, 802, 1099, 1865
AlwNI	CAGNNNCTG	1 cut(s)	139
Ama87I	CYCGRG	3 cut(s)	739, 1500, 1754
Aor13HI	TCCGGA	1 cut(s)	770
Aor51HI	AGCGCT	1 cut(s)	1989
AoxI	GGCC	5 cut(s)	457, 802, 831, 1875, 1961
ApeKI	GCWGC	4 cut(s)	80, 1022, 1268, 1619
ApoI	RAATTY	5 cut(s)	355, 1056, 1178, 1691, 1955
AsiGI	ACCGGT	1 cut(s)	173
Asp700I	GAANNNNTTC	1 cut(s)	344
AspLEI	GCGC	4 cut(s)	1326, 1367, 1933, 1990
AspS9I	GGNCC	3 cut(s)	701, 1993, 2058
AsuC2I	CCSGG	3 cut(s)	265, 740, 741
AsuHPI	GGTGA	6 cut(s)	206, 446, 1104, 1202, 1644, 1759
AvaI	CYCGRG	3 cut(s)	739, 1500, 1754
AvaII	GGWCC	3 cut(s)	701, 1993, 2058
BalI	TGGCCA	1 cut(s)	804
BanI	GGYRCC	1 cut(s)	101
BanII	GRGCYC	2 cut(s)	239, 1590
BarI	GAAGNNNNNNTAC	2 cut(s)	1515, 1547
BbsI	GAAGAC	1 cut(s)	1376
Bbv12I	GWGCWC	3 cut(s)	130, 1590, 1687
BbvI	GCAGC	4 cut(s)	67, 1009, 1280, 1606
BccI	CCATC	6 cut(s)	630, 674, 794, 1439, 1654, 1815
BceAI	ACGGC	2 cut(s)	393, 818
BciVI	GTATCC	3 cut(s)	284, 633, 1902
BclI	TGATCA	1 cut(s)	7
BcnI	CCSGG	3 cut(s)	265, 740, 741
BcoDI	GTCTC	3 cut(s)	278, 967, 1908
BfaI	CTAG	3 cut(s)	1388, 1781, 1856
BfmI	CTRYAG	2 cut(s)	1137, 1732
BfoI	RGCGCY	1 cut(s)	1991
BfrI	CTTAAG	1 cut(s)	514
BfuAI	ACCTGC	1 cut(s)	315
BfuI	GTATCC	3 cut(s)	284, 633, 1902
BglI	GCCNNNNNGGC	1 cut(s)	839
BglII	AGATCT	1 cut(s)	746
BisI	GCNGC	5 cut(s)	81, 1020, 1023, 1269, 1620
BlpI	GCTNAGC	1 cut(s)	238
BlsI	GCNGC	5 cut(s)	82, 1021, 1024, 1270, 1621
BmcAI	AGTACT	1 cut(s)	1514
Bme1390I	CCNGG	3 cut(s)	265, 740, 741
Bme18I	GGWCC	3 cut(s)	701, 1993, 2058
BmeT110I	CYCGRG	3 cut(s)	739, 1500, 1754
BmgBI	CACGTC	1 cut(s)	1208
BmgT120I	GGNCC	3 cut(s)	701, 1993, 2058
BmiI	GGNNCC	3 cut(s)	103, 1950, 2060
BmrFI	CCNGG	3 cut(s)	265, 740, 741
BmrI	ACTGGG	1 cut(s)	418
BmsI	GCATC	4 cut(s)	100, 984, 1342, 1740
BmuI	ACTGGG	1 cut(s)	418
BpiI	GAAGAC	1 cut(s)	1376
Bpu1102I	GCTNAGC	1 cut(s)	238
BpuEI	CTTGAG	1 cut(s)	1348
BpuMI	CCSGG	3 cut(s)	265, 740, 741
Bsa29I	ATCGAT	1 cut(s)	1094
BsaHI	GRCGYC	3 cut(s)	1728, 1851, 1923
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	3 cut(s)	468, 738, 739
BsaWI	WCCGGW	2 cut(s)	173, 770
BsaXI	ACNNNNNCTCC	2 cut(s)	1111, 1141
Bsc4I	CCNNNNNNNGG	4 cut(s)	740, 791, 1479, 1480
Bse118I	RCCGGY	1 cut(s)	173
Bse1I	ACTGG	6 cut(s)	355, 424, 495, 571, 1356, 1771
Bse3DI	GCAATG	2 cut(s)	1277, 1926
BseAI	TCCGGA	1 cut(s)	770
BseCI	ATCGAT	1 cut(s)	1094
BseDI	CCNNGG	3 cut(s)	468, 738, 739
BseGI	GGATG	1 cut(s)	641
BseLI	CCNNNNNNNGG	4 cut(s)	740, 791, 1479, 1480
BseMI	GCAATG	2 cut(s)	1277, 1926
BseMII	CTCAG	8 cut(s)	42, 162, 179, 252, 289, 403, 981, 1670
BseNI	ACTGG	6 cut(s)	355, 424, 495, 571, 1356, 1771
BseRI	GAGGAG	1 cut(s)	699
BseXI	GCAGC	4 cut(s)	67, 1009, 1280, 1606
Bsh1236I	CGCG	1 cut(s)	1324
BshFI	GGCC	5 cut(s)	459, 804, 833, 1877, 1963
BshNI	GGYRCC	1 cut(s)	101
BshTI	ACCGGT	1 cut(s)	173
BshVI	ATCGAT	1 cut(s)	1094
BsiHKAI	GWGCWC	3 cut(s)	130, 1590, 1687
BsiHKCI	CYCGRG	3 cut(s)	739, 1500, 1754
BsiSI	CCGG	7 cut(s)	174, 264, 740, 771, 1874, 1946, 1960
BslI	CCNNNNNNNGG	4 cut(s)	740, 791, 1479, 1480
BsmAI	GTCTC	3 cut(s)	278, 967, 1908
BsmI	GAATGC	1 cut(s)	1474
BsnI	GGCC	5 cut(s)	459, 804, 833, 1877, 1963
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	3 cut(s)	739, 1500, 1754
Bsp1286I	GDGCHC	4 cut(s)	130, 239, 1590, 1687
Bsp13I	TCCGGA	1 cut(s)	770
Bsp1407I	TGTACA	2 cut(s)	141, 1543
Bsp1720I	GCTNAGC	1 cut(s)	238
BspACI	CCGC	4 cut(s)	192, 1019, 1050, 1996
BspANI	GGCC	5 cut(s)	459, 804, 833, 1877, 1963
BspCNI	CTCAG	8 cut(s)	43, 161, 178, 251, 288, 402, 982, 1669
BspDI	ATCGAT	1 cut(s)	1094
BspEI	TCCGGA	1 cut(s)	770
BspFNI	CGCG	1 cut(s)	1324
BspLI	GGNNCC	3 cut(s)	103, 1950, 2060
BspMI	ACCTGC	1 cut(s)	315
BspPI	GGATC	6 cut(s)	191, 480, 781, 802, 1099, 1865
BspQI	GCTCTTC	1 cut(s)	1578
BspT107I	GGYRCC	1 cut(s)	101
BspTI	CTTAAG	1 cut(s)	514
BspTNI	GGTCTC	1 cut(s)	278
BsrDI	GCAATG	2 cut(s)	1277, 1926
BsrFI	RCCGGY	1 cut(s)	173
BsrGI	TGTACA	2 cut(s)	141, 1543
BsrI	ACTGG	6 cut(s)	355, 424, 495, 571, 1356, 1771
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	3 cut(s)	468, 738, 739
BssNAI	GTATAC	1 cut(s)	1711
BssNI	GRCGYC	3 cut(s)	1728, 1851, 1923
BssT1I	CCWWGG	1 cut(s)	468
Bst1107I	GTATAC	1 cut(s)	1711
Bst4CI	ACNGT	7 cut(s)	141, 158, 1138, 1610, 1717, 1787, 1809
Bst6I	CTCTTC	1 cut(s)	1578
BstACI	GRCGYC	3 cut(s)	1728, 1851, 1923
BstAFI	CTTAAG	1 cut(s)	514
BstAUI	TGTACA	2 cut(s)	141, 1543
BstC8I	GCNNGC	7 cut(s)	44, 457, 831, 970, 1418, 1986, 2014
BstF5I	GGATG	1 cut(s)	641
BstFNI	CGCG	1 cut(s)	1324
BstH2I	RGCGCY	1 cut(s)	1991
BstHHI	GCGC	4 cut(s)	1326, 1367, 1933, 1990
BstMAI	GTCTC	3 cut(s)	278, 967, 1908
BstMWI	GCNNNNNNNGC	3 cut(s)	452, 839, 1330
BstNSI	RCATGY	2 cut(s)	1891, 2086
BstSCI	CCNGG	3 cut(s)	263, 738, 739
BstSFI	CTRYAG	2 cut(s)	1137, 1732
BstUI	CGCG	1 cut(s)	1324
BstV1I	GCAGC	4 cut(s)	67, 1009, 1280, 1606
BstV2I	GAAGAC	1 cut(s)	1376
BstX2I	RGATCY	4 cut(s)	472, 746, 773, 794
BstXI	CCANNNNNNTGG	2 cut(s)	431, 632
BstYI	RGATCY	4 cut(s)	472, 746, 773, 794
BstZ17I	GTATAC	1 cut(s)	1711
Bsu15I	ATCGAT	1 cut(s)	1094
BsuI	GTATCC	3 cut(s)	284, 633, 1902
BsuRI	GGCC	5 cut(s)	459, 804, 833, 1877, 1963
BsuTUI	ATCGAT	1 cut(s)	1094
BtrI	CACGTC	1 cut(s)	1208
BtsCI	GGATG	1 cut(s)	641
BtsI	GCAGTG	2 cut(s)	1119, 1385
BtsIMutI	CAGTG	9 cut(s)	48, 283, 348, 502, 637, 1119, 1349, 1385, 1713
BveI	ACCTGC	1 cut(s)	315
Cac8I	GCNNGC	7 cut(s)	44, 457, 831, 970, 1418, 1986, 2014
CaiI	CAGNNNCTG	1 cut(s)	139
CfoI	GCGC	4 cut(s)	1326, 1367, 1933, 1990
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	3 cut(s)	701, 1993, 2058
Cfr9I	CCCGGG	1 cut(s)	739
ClaI	ATCGAT	1 cut(s)	1094
CseI	GACGC	1 cut(s)	1859
Csp6I	GTAC	6 cut(s)	137, 142, 570, 666, 1513, 1544
CspAI	ACCGGT	1 cut(s)	173
CviQI	GTAC	6 cut(s)	137, 142, 570, 666, 1513, 1544
DraI	TTTAAA	1 cut(s)	609
EaeI	YGGCCR	2 cut(s)	802, 831
Eam1104I	CTCTTC	1 cut(s)	1578
EarI	CTCTTC	1 cut(s)	1578
Ecl136II	GAGCTC	1 cut(s)	1588
Eco130I	CCWWGG	1 cut(s)	468
Eco24I	GRGCYC	2 cut(s)	239, 1590
Eco31I	GGTCTC	1 cut(s)	278
Eco47I	GGWCC	3 cut(s)	701, 1993, 2058
Eco47III	AGCGCT	1 cut(s)	1989
Eco53kI	GAGCTC	1 cut(s)	1588
Eco57I	CTGAAG	3 cut(s)	116, 1153, 1461
Eco88I	CYCGRG	3 cut(s)	739, 1500, 1754
EcoICRI	GAGCTC	1 cut(s)	1588
EcoO109I	RGGNCCY	1 cut(s)	2058
EcoRI	GAATTC	2 cut(s)	1691, 1955
EcoT14I	CCWWGG	1 cut(s)	468
EcoT38I	GRGCYC	2 cut(s)	239, 1590
ErhI	CCWWGG	1 cut(s)	468
FalI	AAGNNNNNCTT	4 cut(s)	1167, 1199, 1185, 1217
FauI	CCCGC	1 cut(s)	1057
FbaI	TGATCA	1 cut(s)	7
FblI	GTMKAC	2 cut(s)	1710, 1731
Fnu4HI	GCNGC	5 cut(s)	81, 1020, 1023, 1269, 1620
FokI	GGATG	1 cut(s)	648
FriOI	GRGCYC	2 cut(s)	239, 1590
Fsp4HI	GCNGC	5 cut(s)	81, 1020, 1023, 1269, 1620
FspBI	CTAG	3 cut(s)	1388, 1781, 1856
FspI	TGCGCA	1 cut(s)	1366
GlaI	GCGC	4 cut(s)	1325, 1366, 1932, 1989
GluI	GCNGC	5 cut(s)	81, 1020, 1023, 1269, 1620
HaeII	RGCGCY	1 cut(s)	1991
HaeIII	GGCC	5 cut(s)	459, 804, 833, 1877, 1963
HapII	CCGG	7 cut(s)	174, 264, 740, 771, 1874, 1946, 1960
HgaI	GACGC	1 cut(s)	1859
HhaI	GCGC	4 cut(s)	1326, 1367, 1933, 1990
Hin1I	GRCGYC	3 cut(s)	1728, 1851, 1923
Hin6I	GCGC	4 cut(s)	1324, 1365, 1931, 1988
HinP1I	GCGC	4 cut(s)	1324, 1365, 1931, 1988
HincII	GTYRAC	1 cut(s)	1764
HindII	GTYRAC	1 cut(s)	1764
HinfI	GANTC	8 cut(s)	23, 37, 209, 757, 815, 937, 1456, 2039
HpaII	CCGG	7 cut(s)	174, 264, 740, 771, 1874, 1946, 1960
HphI	GGTGA	6 cut(s)	206, 446, 1104, 1202, 1644, 1759
Hpy166II	GTNNAC	6 cut(s)	144, 154, 1213, 1711, 1732, 1764
Hpy188III	TCNNGA	6 cut(s)	771, 777, 1175, 1688, 1704, 2069
Hpy8I	GTNNAC	6 cut(s)	144, 154, 1213, 1711, 1732, 1764
HpyAV	CCTTC	4 cut(s)	24, 171, 1187, 1545
HpyCH4III	ACNGT	7 cut(s)	141, 158, 1138, 1610, 1717, 1787, 1809
HpyCH4IV	ACGT	5 cut(s)	1207, 1728, 1912, 1923, 2026
HpyF10VI	GCNNNNNNNGC	3 cut(s)	452, 839, 1330
HpySE526I	ACGT	5 cut(s)	1207, 1728, 1912, 1923, 2026
Hsp92I	GRCGYC	3 cut(s)	1728, 1851, 1923
HspAI	GCGC	4 cut(s)	1324, 1365, 1931, 1988
Kpn2I	TCCGGA	1 cut(s)	770
Ksp22I	TGATCA	1 cut(s)	7
LguI	GCTCTTC	1 cut(s)	1578
LmnI	GCTCC	4 cut(s)	712, 1413, 1690, 1948
Lsp1109I	GCAGC	4 cut(s)	67, 1009, 1280, 1606
LweI	GCATC	4 cut(s)	100, 984, 1342, 1740
MaeI	CTAG	3 cut(s)	1388, 1781, 1856
MaeII	ACGT	5 cut(s)	1207, 1728, 1912, 1923, 2026
MboII	GAAGA	7 cut(s)	528, 756, 1301, 1376, 1421, 1454, 1595
MfeI	CAATTG	2 cut(s)	429, 2076
MflI	RGATCY	4 cut(s)	472, 746, 773, 794
MhlI	GDGCHC	4 cut(s)	130, 239, 1590, 1687
MlsI	TGGCCA	1 cut(s)	804
MluNI	TGGCCA	1 cut(s)	804
MlyI	GAGTC	4 cut(s)	17, 31, 218, 1450
MmeI	TCCRAC	3 cut(s)	158, 327, 1751
Mox20I	TGGCCA	1 cut(s)	804
MroI	TCCGGA	1 cut(s)	770
MroXI	GAANNNNTTC	1 cut(s)	344
MscI	TGGCCA	1 cut(s)	804
MseI	TTAA	7 cut(s)	320, 483, 515, 608, 1251, 1880, 2092
MslI	CAYNNNNRTG	1 cut(s)	630
Msp20I	TGGCCA	1 cut(s)	804
MspA1I	CMGCKG	1 cut(s)	242
MspCI	CTTAAG	1 cut(s)	514
MspI	CCGG	7 cut(s)	174, 264, 740, 771, 1874, 1946, 1960
MspR9I	CCNGG	3 cut(s)	265, 740, 741
MunI	CAATTG	2 cut(s)	429, 2076
Mva1269I	GAATGC	1 cut(s)	1474
MvnI	CGCG	1 cut(s)	1324
MwoI	GCNNNNNNNGC	3 cut(s)	452, 839, 1330
NciI	CCSGG	3 cut(s)	265, 740, 741
NlaIV	GGNNCC	3 cut(s)	103, 1950, 2060
NmuCI	GTSAC	5 cut(s)	34, 434, 1724, 1809, 2033
NsbI	TGCGCA	1 cut(s)	1366
NspI	RCATGY	2 cut(s)	1891, 2086
PaeR7I	CTCGAG	2 cut(s)	1500, 1754
PciSI	GCTCTTC	1 cut(s)	1578
PctI	GAATGC	1 cut(s)	1474
PdmI	GAANNNNTTC	1 cut(s)	344
PfeI	GAWTC	4 cut(s)	757, 815, 937, 2039
PinAI	ACCGGT	1 cut(s)	173
PkrI	GCNGC	5 cut(s)	82, 1021, 1024, 1270, 1621
PleI	GAGTC	4 cut(s)	17, 31, 217, 1450
PpsI	GAGTC	4 cut(s)	17, 31, 217, 1450
PpuMI	RGGWCCY	1 cut(s)	2058
Psp124BI	GAGCTC	1 cut(s)	1590
Psp5II	RGGWCCY	1 cut(s)	2058
PspN4I	GGNNCC	3 cut(s)	103, 1950, 2060
PspPI	GGNCC	3 cut(s)	701, 1993, 2058
PspPPI	RGGWCCY	1 cut(s)	2058
PspXI	VCTCGAGB	2 cut(s)	1500, 1754
PstNI	CAGNNNCTG	1 cut(s)	139
PsuI	RGATCY	4 cut(s)	472, 746, 773, 794
PvuII	CAGCTG	1 cut(s)	242
RsaI	GTAC	6 cut(s)	138, 143, 571, 667, 1514, 1545
RsaNI	GTAC	6 cut(s)	137, 142, 570, 666, 1513, 1544
RseI	CAYNNNNRTG	1 cut(s)	630
SacI	GAGCTC	1 cut(s)	1590
SapI	GCTCTTC	1 cut(s)	1578
SaqAI	TTAA	7 cut(s)	320, 483, 515, 608, 1251, 1880, 2092
SatI	GCNGC	5 cut(s)	81, 1020, 1023, 1269, 1620
Sau96I	GGNCC	3 cut(s)	701, 1993, 2058
ScaI	AGTACT	1 cut(s)	1514
SchI	GAGTC	4 cut(s)	17, 31, 218, 1450
ScrFI	CCNGG	3 cut(s)	265, 740, 741
SduI	GDGCHC	4 cut(s)	130, 239, 1590, 1687
SfaNI	GCATC	4 cut(s)	100, 984, 1342, 1740
SfcI	CTRYAG	2 cut(s)	1137, 1732
Sfr274I	CTCGAG	2 cut(s)	1500, 1754
SinI	GGWCC	3 cut(s)	701, 1993, 2058
SlaI	CTCGAG	2 cut(s)	1500, 1754
SmaI	CCCGGG	1 cut(s)	741
SmiMI	CAYNNNNRTG	1 cut(s)	630
SmlI	CTYRAG	4 cut(s)	514, 1327, 1500, 1754
SmoI	CTYRAG	4 cut(s)	514, 1327, 1500, 1754
SsiI	CCGC	4 cut(s)	192, 1019, 1050, 1996
SspI	AATATT	1 cut(s)	1570
SspMI	CTAG	3 cut(s)	1388, 1781, 1856
SstI	GAGCTC	1 cut(s)	1590
StyD4I	CCNGG	3 cut(s)	263, 738, 739
StyI	CCWWGG	1 cut(s)	468
TaaI	ACNGT	7 cut(s)	141, 158, 1138, 1610, 1717, 1787, 1809
TaiI	ACGT	5 cut(s)	1210, 1731, 1915, 1926, 2029
TaqI	TCGA	6 cut(s)	71, 124, 1094, 1501, 1755, 1796
TatI	WGTACW	3 cut(s)	141, 1512, 1543
TauI	GCSGC	1 cut(s)	1022
TfiI	GAWTC	4 cut(s)	757, 815, 937, 2039
Tru1I	TTAA	7 cut(s)	320, 483, 515, 608, 1251, 1880, 2092
Tru9I	TTAA	7 cut(s)	320, 483, 515, 608, 1251, 1880, 2092
TscAI	CASTG	9 cut(s)	55, 283, 355, 502, 637, 1126, 1356, 1385, 1720
TseFI	GTSAC	5 cut(s)	34, 434, 1724, 1809, 2033
TseI	GCWGC	4 cut(s)	80, 1022, 1268, 1619
Tsp45I	GTSAC	5 cut(s)	34, 434, 1724, 1809, 2033
TspDTI	ATGAA	5 cut(s)	510, 1421, 1536, 1596, 1659
TspGWI	ACGGA	1 cut(s)	189
TspMI	CCCGGG	1 cut(s)	739
TspRI	CASTG	9 cut(s)	55, 283, 355, 502, 637, 1126, 1356, 1385, 1720
Vha464I	CTTAAG	1 cut(s)	514
VpaK11BI	GGWCC	3 cut(s)	701, 1993, 2058
XapI	RAATTY	5 cut(s)	355, 1056, 1178, 1691, 1955
XceI	RCATGY	2 cut(s)	1891, 2086
XhoI	CTCGAG	2 cut(s)	1500, 1754
XmaI	CCCGGG	1 cut(s)	739
XmiI	GTMKAC	2 cut(s)	1710, 1731
XmnI	GAANNNNTTC	1 cut(s)	344
XspI	CTAG	3 cut(s)	1388, 1781, 1856
ZraI	GACGTC	2 cut(s)	1729, 1924
ZrmI	AGTACT	1 cut(s)	1514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.