Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010533

Physical Location & Seq

Genomic Coordinates Forward (+)

47908808 .. 47911876

Gene Structure 3069 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1364100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGGAGGAGGAGGAGGGGCATCATCAAAACTGAGAAAAGCAGCAAAGAAAATGGCGGTGGCTGCTGCAAATGCATGTGGTGGTTCCTTTTCCCGCAGGAAGACCATCGTCTCCGATCATACCAACACTACCACTCCCACTATCTCAGGTTCTTCAGCCGTGTCTCCAACAAACCCAACGAATGTCCAACCAGCAACAGCAGCAGCAAAAGAAGAAGAAGTTTCCAAAGAAAGGGAAATGACAATCAATTATAGCAATGTGCCTTATGCTAAGAGTTTGTGCGCAATATGTCTAGACCCTCTGAGTTACCATAGCAAGGGGAGCAGCCCAGGGCAGGCTATATTCACGGCCCAGTGCTCCCATGCTTTCCACTTTTCCTGCATCTCCTCCAATGTTCGCCATGGTAGTGTTACCTGCCCCATTTGCCGAGCCCATTGGACCCAGCTGCCCCGCAATCTGAACCCACCTTGGGGTTCCCTGTCTTCTTGCAACCGAGCTGACCCTATCTTCCAAATCCTGGATGACTCTATTGCCACTTTCCGCATCCACAGGCGCTCATTCTTGCGCTCTGCTCACTATGATGATGACGACCCAATTGAGCCCGATCACGTCTCTAGTTTCCCCCGTCTCCAATTCTCTTTGATACCCATCCCACATAGTGCACCCCCTAGTTTGTTTACTCCATATTCATACCATCCGCCCCCACATCACCTAAGCTGTAGCTCCTCATCATTGTTACAATCACCAACCAGGCCAAAGTCTTATACTATGTGTGCCTTCTCAGACAGAGCATACCTTTCTGTGAAATTAGCAAATCAACGAGCAACAGACTTGGTGTTGGTTGCTAGCCCCAATGGGCCGCACTTGAGGCTTCTCAAGCAGTGCATGGCATTGGTGGTTTTTTCCCTTAGACCAATTGATCGTTTGGCCATTGTTACCTACTCATCAGCTGCTGCTCGCGTCTTCCCTTTGAGACGCATGACATCTTATGGAAAGCGAACAGCCCAACAGGTTATTGATCGTCTGTTCTACATGGGGCAAGCAGATCCCGTGGAAGGGATCAAGAAGGGGATAAAGATACTTGAAGATCGTGCATACAAGAACCCAGAGTGTGGCATCCTGCATCTGTCTGACAGCCCAATGCGATCCTACCATGCTGCAATGGACATGGATGTGCCGATTCCAGTCCATCGCTTCCATGTAGGATTTGGATTTGGCACTTCGAATGGATATATCATGCATGAGTTTGAAGATTTGCTAAGAAGAATCCTAGGCGGCATGATTAGAGAAATTGAGTTGAGGATGAGAATAGGGGAGGAAGCCAGCAGAAGCAGTCGGATCGTGAGAATAGGAGAGCTAAGAGGAGGTGAAGAAAAGAAAATCTTGGTGGACTTGGGCGTGTGTGAACATATTTGTGTGGGATATAGCTACATTGAGGAGGGTGATGAGATGACAACAGGGGAAACAGTGGCGAAGGTAGGACTAGGAGATGATGTTGGTAAGAGTAGTAGTAGTAGTAGTAGTAATGCAACGGTTAGAGGAGGACGGGGAGGATTTGCTTGTGGTGCTGCATCAGGGACGAGTGGTGCAATCATCGGCGGGAGGTCTAGTACAAGTACTAGTAGTGTAGAAAGTTGGGACTACCATGACGCTTACATGGCTAGAAGATGGGCCAAGCATTTGCATGCCTACAGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA_2 zf-C3HC4 zf-RING_2 zf-rbx1

Protein Analysis

567

Amino Acids

61.72

Weight (kDa)

9.1

Isoelectric Point (pI)

55.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	93 - 143	1.6e-07	Ring finger domain
zf-rbx1	PF12678	93 - 143	4.2e-07	RING-H2 zinc finger domain
zf-C3HC4	PF00097	94 - 142	1.3e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	94 - 128	1.4e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00090 FvH4_3g00090
malus_domestica	MD05G1364100.v1.1
prunus_persica	Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1
pyrus_communis	pycom05g33030
rosa_chinensis	RchiOBHm_Chr5g0000121
rosa_laevigata	RLG00000030843
rosa_multiflora	Rmu_sc0003125.1_g000002 Rmu_ssc0000243.1_g000019
rosa_roxburghii	Rroxscaffold_1G00075980
rosa_rugosa	Rorug04G0381700
rosa_samantha	Rh5AG000800 Rh5BG002000 Rh5CG000900 Rh5DG000800
rosa_wichuraiana	Rw5G000110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	283
Acc36I	ACCTGC	1 cut(s)	422
AccB7I	CCANNNNNTGG	2 cut(s)	517, 1112
AccII	CGCG	1 cut(s)	960
AciI	CCGC	8 cut(s)	56, 94, 451, 541, 698, 860, 1275, 1599
AclWI	GGATC	4 cut(s)	1040, 1067, 1140, 1346
AcoI	YGGCCR	1 cut(s)	927
AcuI	CTGAAG	1 cut(s)	138
AdeI	CACNNNGTG	1 cut(s)	659
AfaI	GTAC	2 cut(s)	1612, 1618
AfiI	CCNNNNNNNGG	9 cut(s)	231, 316, 334, 469, 470, 517, 1055, 1056, 1112
AgsI	TTSAA	2 cut(s)	1085, 1250
AhlI	ACTAGT	1 cut(s)	1619
AjiI	CACGTC	1 cut(s)	610
AjnI	CCWGG	3 cut(s)	328, 516, 749
AluBI	AGCT	7 cut(s)	445, 497, 717, 723, 950, 1357, 1428
AluI	AGCT	7 cut(s)	445, 497, 717, 723, 950, 1357, 1428
Alw21I	GWGCWC	2 cut(s)	359, 664
Alw26I	GTCTC	5 cut(s)	115, 168, 616, 632, 967
Alw44I	GTGCAC	1 cut(s)	660
AlwI	GGATC	4 cut(s)	1040, 1067, 1140, 1346
AlwNI	CAGNNNCTG	1 cut(s)	953
AoxI	GGCC	5 cut(s)	348, 752, 857, 927, 1671
ApaLI	GTGCAC	1 cut(s)	660
AspA2I	CCTAGG	1 cut(s)	1270
AspLEI	GCGC	3 cut(s)	284, 555, 567
AspS9I	GGNCC	4 cut(s)	349, 438, 857, 1671
AsuHPI	GGTGA	4 cut(s)	701, 735, 1379, 1454
AsuII	TTCGAA	1 cut(s)	1223
AsuNHI	GCTAGC	1 cut(s)	845
AvaII	GGWCC	1 cut(s)	438
AvrII	CCTAGG	1 cut(s)	1270
BaeGI	GKGCMC	1 cut(s)	664
BalI	TGGCCA	1 cut(s)	929
BanII	GRGCYC	2 cut(s)	433, 603
BbsI	GAAGAC	3 cut(s)	107, 474, 955
Bbv12I	GWGCWC	2 cut(s)	359, 664
BccI	CCATC	5 cut(s)	113, 656, 702, 1197, 1662
BceAI	ACGGC	2 cut(s)	143, 363
BcgI	CGANNNNNNTGC	2 cut(s)	1321, 1355
BciT130I	CCWGG	3 cut(s)	330, 518, 751
BcoDI	GTCTC	5 cut(s)	115, 168, 616, 632, 967
BcuI	ACTAGT	1 cut(s)	1619
BfaI	CTAG	9 cut(s)	293, 615, 669, 846, 1271, 1484, 1608, 1620, 1662
BfmI	CTRYAG	2 cut(s)	718, 1690
BfoI	RGCGCY	1 cut(s)	556
BfuAI	ACCTGC	1 cut(s)	422
BlnI	CCTAGG	1 cut(s)	1270
BmcAI	AGTACT	1 cut(s)	1618
Bme1390I	CCNGG	3 cut(s)	330, 518, 751
Bme18I	GGWCC	1 cut(s)	438
BmgBI	CACGTC	1 cut(s)	610
BmgT120I	GGNCC	4 cut(s)	349, 438, 857, 1671
BmiI	GGNNCC	3 cut(s)	85, 440, 475
BmrFI	CCNGG	3 cut(s)	330, 518, 751
BmrI	ACTGGG	1 cut(s)	346
BmsI	GCATC	6 cut(s)	29, 390, 552, 1125, 1132, 1580
BmtI	GCTAGC	1 cut(s)	849
BmuI	ACTGGG	1 cut(s)	346
BoxI	GACNNNNGTC	1 cut(s)	107
BpiI	GAAGAC	3 cut(s)	107, 474, 955
Bpu10I	CCTNAGC	1 cut(s)	713
Bpu14I	TTCGAA	1 cut(s)	1223
BpuEI	CTTGAG	2 cut(s)	860, 886
BsaBI	GATNNNNATC	1 cut(s)	647
BsaJI	CCNNGG	6 cut(s)	328, 329, 400, 467, 1050, 1270
BsaXI	ACNNNNNCTCC	6 cut(s)	118, 148, 1479, 1509, 1594, 1624
Bsc4I	CCNNNNNNNGG	9 cut(s)	231, 316, 334, 469, 470, 517, 1055, 1056, 1112
Bse1I	ACTGG	2 cut(s)	352, 1184
Bse3DI	GCAATG	2 cut(s)	262, 1167
Bse8I	GATNNNNATC	1 cut(s)	647
BseBI	CCWGG	3 cut(s)	330, 518, 751
BseDI	CCNNGG	6 cut(s)	328, 329, 400, 467, 1050, 1270
BseGI	GGATG	7 cut(s)	526, 543, 648, 694, 1116, 1177, 1308
BseJI	GATNNNNATC	1 cut(s)	647
BseLI	CCNNNNNNNGG	9 cut(s)	231, 316, 334, 469, 470, 517, 1055, 1056, 1112
BseMI	GCAATG	2 cut(s)	262, 1167
BseMII	CTCAG	4 cut(s)	23, 159, 293, 795
BseNI	ACTGG	2 cut(s)	352, 1184
BseRI	GAGGAG	8 cut(s)	21, 24, 27, 376, 715, 1377, 1451, 1554
BseSI	GKGCMC	1 cut(s)	664
BseYI	CCCAGC	1 cut(s)	441
Bsh1236I	CGCG	1 cut(s)	960
BshFI	GGCC	5 cut(s)	350, 754, 859, 929, 1673
BsiHKAI	GWGCWC	2 cut(s)	359, 664
BslFI	GGGAC	2 cut(s)	1591, 1652
BslI	CCNNNNNNNGG	9 cut(s)	231, 316, 334, 469, 470, 517, 1055, 1056, 1112
BsmAI	GTCTC	5 cut(s)	115, 168, 616, 632, 967
BsmBI	CGTCTC	4 cut(s)	115, 616, 632, 967
BsmFI	GGGAC	2 cut(s)	1591, 1652
BsnI	GGCC	5 cut(s)	350, 754, 859, 929, 1673
Bsp119I	TTCGAA	1 cut(s)	1223
Bsp1286I	GDGCHC	4 cut(s)	359, 433, 603, 664
Bsp143I	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
Bsp19I	CCATGG	1 cut(s)	400
BspACI	CCGC	8 cut(s)	56, 94, 451, 541, 698, 860, 1275, 1599
BspANI	GGCC	5 cut(s)	350, 754, 859, 929, 1673
BspCNI	CTCAG	4 cut(s)	24, 158, 294, 794
BspFNI	CGCG	1 cut(s)	960
BspLI	GGNNCC	3 cut(s)	85, 440, 475
BspMI	ACCTGC	1 cut(s)	422
BspOI	GCTAGC	1 cut(s)	849
BspPI	GGATC	4 cut(s)	1040, 1067, 1140, 1346
BspT104I	TTCGAA	1 cut(s)	1223
BsrDI	GCAATG	2 cut(s)	262, 1167
BsrI	ACTGG	2 cut(s)	352, 1184
BssECI	CCNNGG	6 cut(s)	328, 329, 400, 467, 1050, 1270
BssMI	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
BssT1I	CCWWGG	3 cut(s)	400, 467, 1270
Bst2UI	CCWGG	3 cut(s)	330, 518, 751
Bst4CI	ACNGT	2 cut(s)	1468, 1534
BstBI	TTCGAA	1 cut(s)	1223
BstC8I	GCNNGC	6 cut(s)	336, 847, 958, 1041, 1324, 1686
BstDEI	CTNAG	9 cut(s)	32, 145, 270, 302, 713, 781, 908, 1259, 1358
BstDSI	CCRYGG	2 cut(s)	400, 1050
BstF5I	GGATG	7 cut(s)	526, 543, 648, 694, 1116, 1177, 1308
BstFNI	CGCG	1 cut(s)	960
BstH2I	RGCGCY	1 cut(s)	556
BstHHI	GCGC	3 cut(s)	284, 555, 567
BstKTI	GATC	9 cut(s)	118, 607, 922, 1021, 1048, 1062, 1090, 1148, 1341
BstMAI	GTCTC	5 cut(s)	115, 168, 616, 632, 967
BstMBI	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
BstMWI	GCNNNNNNNGC	9 cut(s)	62, 71, 200, 321, 423, 868, 877, 1565, 1658
BstNI	CCWGG	3 cut(s)	330, 518, 751
BstNSI	RCATGY	2 cut(s)	78, 1688
BstPAI	GACNNNNGTC	1 cut(s)	107
BstSCI	CCNGG	3 cut(s)	328, 516, 749
BstSFI	CTRYAG	2 cut(s)	718, 1690
BstSLI	GKGCMC	1 cut(s)	664
BstUI	CGCG	1 cut(s)	960
BstV2I	GAAGAC	3 cut(s)	107, 474, 955
BstX2I	RGATCY	1 cut(s)	1045
BstYI	RGATCY	1 cut(s)	1045
BsuRI	GGCC	5 cut(s)	350, 754, 859, 929, 1673
BtgI	CCRYGG	2 cut(s)	400, 1050
BtgZI	GCGATG	1 cut(s)	1175
BtrI	CACGTC	1 cut(s)	610
BtsCI	GGATG	7 cut(s)	526, 543, 648, 694, 1116, 1177, 1308
BtsI	GCAGTG	1 cut(s)	887
BtsIMutI	CAGTG	3 cut(s)	359, 887, 1473
BveI	ACCTGC	1 cut(s)	422
Cac8I	GCNNGC	6 cut(s)	336, 847, 958, 1041, 1324, 1686
CaiI	CAGNNNCTG	1 cut(s)	953
CfoI	GCGC	3 cut(s)	284, 555, 567
Cfr13I	GGNCC	4 cut(s)	349, 438, 857, 1671
CseI	GACGC	3 cut(s)	949, 984, 1658
Csp6I	GTAC	2 cut(s)	1611, 1617
CviQI	GTAC	2 cut(s)	1611, 1617
DdeI	CTNAG	9 cut(s)	32, 145, 270, 302, 713, 781, 908, 1259, 1358
DpnI	GATC	9 cut(s)	117, 606, 921, 1020, 1047, 1061, 1089, 1147, 1340
DpnII	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
DraIII	CACNNNGTG	1 cut(s)	659
EaeI	YGGCCR	1 cut(s)	927
EciI	GGCGGA	1 cut(s)	687
Eco130I	CCWWGG	3 cut(s)	400, 467, 1270
Eco24I	GRGCYC	2 cut(s)	433, 603
Eco47I	GGWCC	1 cut(s)	438
Eco57I	CTGAAG	1 cut(s)	138
EcoRII	CCWGG	3 cut(s)	328, 516, 749
EcoT14I	CCWWGG	3 cut(s)	400, 467, 1270
EcoT22I	ATGCAT	2 cut(s)	76, 1242
EcoT38I	GRGCYC	2 cut(s)	433, 603
ErhI	CCWWGG	3 cut(s)	400, 467, 1270
Esp3I	CGTCTC	4 cut(s)	115, 616, 632, 967
FalI	AAGNNNNNCTT	2 cut(s)	1367, 1399
FaqI	GGGAC	2 cut(s)	1591, 1652
FauI	CCCGC	3 cut(s)	101, 458, 1592
FokI	GGATG	7 cut(s)	530, 533, 635, 681, 1103, 1184, 1315
FriOI	GRGCYC	2 cut(s)	433, 603
FspBI	CTAG	9 cut(s)	293, 615, 669, 846, 1271, 1484, 1608, 1620, 1662
FspI	TGCGCA	1 cut(s)	283
GlaI	GCGC	3 cut(s)	283, 554, 566
GsaI	CCCAGC	1 cut(s)	445
HaeII	RGCGCY	1 cut(s)	556
HaeIII	GGCC	5 cut(s)	350, 754, 859, 929, 1673
HgaI	GACGC	3 cut(s)	949, 984, 1658
HhaI	GCGC	3 cut(s)	284, 555, 567
Hin6I	GCGC	3 cut(s)	282, 553, 565
HinP1I	GCGC	3 cut(s)	282, 553, 565
HinfI	GANTC	3 cut(s)	524, 1180, 1266
HphI	GGTGA	4 cut(s)	701, 735, 1379, 1454
Hpy166II	GTNNAC	4 cut(s)	662, 678, 1390, 1406
Hpy188I	TCNGA	6 cut(s)	115, 303, 459, 784, 1132, 1338
Hpy188III	TCNNGA	3 cut(s)	293, 1063, 1342
Hpy8I	GTNNAC	4 cut(s)	662, 678, 1390, 1406
HpyAV	CCTTC	4 cut(s)	787, 1049, 1060, 1468
HpyCH4III	ACNGT	2 cut(s)	1468, 1534
HpyCH4IV	ACGT	1 cut(s)	609
HpyF10VI	GCNNNNNNNGC	9 cut(s)	62, 71, 200, 321, 423, 868, 877, 1565, 1658
HpyF3I	CTNAG	9 cut(s)	32, 145, 270, 302, 713, 781, 908, 1259, 1358
HpySE526I	ACGT	1 cut(s)	609
HspAI	GCGC	3 cut(s)	282, 553, 565
Kzo9I	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
LmnI	GCTCC	3 cut(s)	321, 362, 728
LweI	GCATC	6 cut(s)	29, 390, 552, 1125, 1132, 1580
MaeI	CTAG	9 cut(s)	293, 615, 669, 846, 1271, 1484, 1608, 1620, 1662
MaeII	ACGT	1 cut(s)	609
MaeIII	GTNAC	4 cut(s)	305, 409, 735, 934
MalI	GATC	9 cut(s)	117, 606, 921, 1020, 1047, 1061, 1089, 1147, 1340
MboI	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
MfeI	CAATTG	2 cut(s)	594, 915
MflI	RGATCY	1 cut(s)	1045
MhlI	GDGCHC	4 cut(s)	359, 433, 603, 664
MlsI	TGGCCA	1 cut(s)	929
MluCI	AATT	6 cut(s)	247, 594, 632, 806, 915, 1290
MluNI	TGGCCA	1 cut(s)	929
MlyI	GAGTC	1 cut(s)	518
MmeI	TCCRAC	3 cut(s)	191, 211, 1316
Mox20I	TGGCCA	1 cut(s)	929
Mph1103I	ATGCAT	2 cut(s)	76, 1242
MscI	TGGCCA	1 cut(s)	929
MslI	CAYNNNNRTG	5 cut(s)	405, 579, 1172, 1413, 1683
Msp20I	TGGCCA	1 cut(s)	929
MspA1I	CMGCKG	2 cut(s)	445, 950
MspR9I	CCNGG	3 cut(s)	330, 518, 751
MunI	CAATTG	2 cut(s)	594, 915
MvaI	CCWGG	3 cut(s)	330, 518, 751
MvnI	CGCG	1 cut(s)	960
MwoI	GCNNNNNNNGC	9 cut(s)	62, 71, 200, 321, 423, 868, 877, 1565, 1658
NcoI	CCATGG	1 cut(s)	400
NdeII	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
NheI	GCTAGC	1 cut(s)	845
NlaIV	GGNNCC	3 cut(s)	85, 440, 475
NmeAIII	GCCGAG	1 cut(s)	452
NsbI	TGCGCA	1 cut(s)	283
NsiI	ATGCAT	2 cut(s)	76, 1242
NspI	RCATGY	2 cut(s)	78, 1688
NspV	TTCGAA	1 cut(s)	1223
PaeI	GCATGC	1 cut(s)	1688
PasI	CCCWGGG	1 cut(s)	329
PfeI	GAWTC	2 cut(s)	1180, 1266
PflMI	CCANNNNNTGG	2 cut(s)	517, 1112
PfoI	TCCNGGA	1 cut(s)	516
PleI	GAGTC	1 cut(s)	518
PpsI	GAGTC	1 cut(s)	518
PshAI	GACNNNNGTC	1 cut(s)	107
Psp6I	CCWGG	3 cut(s)	328, 516, 749
PspFI	CCCAGC	1 cut(s)	441
PspGI	CCWGG	3 cut(s)	328, 516, 749
PspN4I	GGNNCC	3 cut(s)	85, 440, 475
PspPI	GGNCC	4 cut(s)	349, 438, 857, 1671
PstNI	CAGNNNCTG	1 cut(s)	953
PsuI	RGATCY	1 cut(s)	1045
PvuII	CAGCTG	2 cut(s)	445, 950
RsaI	GTAC	2 cut(s)	1612, 1618
RsaNI	GTAC	2 cut(s)	1611, 1617
RseI	CAYNNNNRTG	5 cut(s)	405, 579, 1172, 1413, 1683
Sau3AI	GATC	9 cut(s)	115, 604, 919, 1018, 1045, 1059, 1087, 1145, 1338
Sau96I	GGNCC	4 cut(s)	349, 438, 857, 1671
ScaI	AGTACT	1 cut(s)	1618
SchI	GAGTC	1 cut(s)	518
ScrFI	CCNGG	3 cut(s)	330, 518, 751
SduI	GDGCHC	4 cut(s)	359, 433, 603, 664
SfaNI	GCATC	6 cut(s)	29, 390, 552, 1125, 1132, 1580
SfcI	CTRYAG	2 cut(s)	718, 1690
SfuI	TTCGAA	1 cut(s)	1223
SinI	GGWCC	1 cut(s)	438
SmiMI	CAYNNNNRTG	5 cut(s)	405, 579, 1172, 1413, 1683
SmlI	CTYRAG	2 cut(s)	865, 875
SmoI	CTYRAG	2 cut(s)	865, 875
SpeI	ACTAGT	1 cut(s)	1619
SphI	GCATGC	1 cut(s)	1688
Sse9I	AATT	6 cut(s)	247, 594, 632, 806, 915, 1290
SsiI	CCGC	8 cut(s)	56, 94, 451, 541, 698, 860, 1275, 1599
SspMI	CTAG	9 cut(s)	293, 615, 669, 846, 1271, 1484, 1608, 1620, 1662
StyD4I	CCNGG	3 cut(s)	328, 516, 749
StyI	CCWWGG	3 cut(s)	400, 467, 1270
TaaI	ACNGT	2 cut(s)	1468, 1534
TaiI	ACGT	1 cut(s)	612
TaqI	TCGA	1 cut(s)	1223
TasI	AATT	6 cut(s)	247, 594, 632, 806, 915, 1290
TatI	WGTACW	2 cut(s)	1610, 1616
TauI	GCSGC	2 cut(s)	862, 1278
TfiI	GAWTC	2 cut(s)	1180, 1266
TscAI	CASTG	3 cut(s)	359, 887, 1473
TspDTI	ATGAA	1 cut(s)	678
TspRI	CASTG	3 cut(s)	359, 887, 1473
Van91I	CCANNNNNTGG	2 cut(s)	517, 1112
VneI	GTGCAC	1 cut(s)	660
VpaK11BI	GGWCC	1 cut(s)	438
XbaI	TCTAGA	1 cut(s)	292
XceI	RCATGY	2 cut(s)	78, 1688
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1205
XmaJI	CCTAGG	1 cut(s)	1270
XspI	CTAG	9 cut(s)	293, 615, 669, 846, 1271, 1484, 1608, 1620, 1662
ZrmI	AGTACT	1 cut(s)	1618
Zsp2I	ATGCAT	2 cut(s)	76, 1242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.