Rorug04G0381700

von Willebrand factor (vWF) type A domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010533
Physical Location & Seq
Reverse (-)
55232640 .. 55234998
2359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0381700.1

Sequence Viewer

Length: 1572 bp
ATGATGATGAGTTGGGTGAGAGAGACAGTCCTCACTCTTCCCGGTGTTTCGTGTACCTTTCTTCTTCTTCTTCTGTTGGCTGTGATCTTCCTCAAGATAGTTCACAAACTATGGTGGACTCCAACTCGAACACAGAGATTCATGGCTTCTCAGGGAATCAGAGGTCCTTCTTACAGACTCATCCATGGAAACAACAAAGAAATCAGCAACATGCTAACGGAAGCCATGAGCAGACCCATACCGAATTTATCACATGATGTACTATCTGCAGTTCAACCTCACATTCACTCATGGACCAAGAGCTATGGGAAGAATTATCTACAATGGCATGGTCTTCAACCAGTTTTGGTCATTACAGAACCTGAGTTAGGCAAGGAGATTCTCAATAACAAATATAGGGTTTATACAAAACAGAAGCCCAGTATCTATGTGAAGAAGCTATTGGGAGACAGCATTTCCATGGCAGAAGGTGAAAAATGGTCAAAACTGAGAAAGATTTCCAACCATGCCTTCCATGGAGACAGCTTAAAAAGTATGATTCCAGACATGATAGCTAGTGCTGAGACGATGCTCGAAGGGTGGAAAAAGCATGAAGGAAAAGAGATTGAGGTGTATGAACATTTTAGGTTATTCACTTCAGAAGTGATTTCTAGGACAGCATTTGGCAGCAGCTATTTAGAAGGGAAGAATATTTTTGACATGTTGATGAAGTTAAGCTCTGTAATATTCAGAACTGCTCACAAACTCAGGGTTCCTGGCATCAGCAAGTTTTATAAAACCAGTGATGAGAAGGAATCAGAGAAGCTTGAGAAAGGAATAGGAGACACCATAATAGAGATTGTTAAGAAAAGAGAGAGGGAGGCAGTGACCGGAGAAGAAGGCAGCTTTGGGACTGATTTTCTTGGATTGCTTTTAAAGGCTCGCCATGCTACCAATGACAAGCAGAGGATTTCAGTGAATGAATTGGTCGAGGAGTGCAAGACATTTTACTTTGCTGGACAAGAAACGACTAACTCTTTGCTTGTTTGGACTGTCTTTCTTCTGGCCCTCCATCCGGAATGGCAAGAGGAAGCAAGAAAGGAGGTCCTGCAGTTATTTGGCAAAAAAACTCCAAATCCTGATGGCCTTGCCAAACTAAAAACGATGAGTATGATCATCAATGAGTCTCTGAGGCTATATCCTCCTGTCATTTCCCTTGTACGAACAGTGGATAAGGAAGTTAAATTGGGAAACCTCATTATACCTGCTAATGTTGAATTGCTTGTCTCAAATCTAGCACTTCATCATGAACCTCAGTTCTGGGGACAAGATGTGAAACATTTCAAACCTGAGAGATTCTCTGAAGGTGTTGCTAGTGCTACTAACAACAACATGGCTGCATTCTTACCCTTTGGAATGGGACCTAGAACTTGTGTTGGCCTCAACTTTGCCACCATTGAAGCAAAGATTGCTCTTTCAATGATTCTACAACGCTACTCCTTCACCCTTTCCCCAGCCTATGTCCACTCGCCCTTTCAGTTTCTCACAGTTCGTCCACAGCATGGACTTCAAGTAATTTTACACTCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_2 zf-C3HC4 zf-RING_2 zf-rbx1

Protein Analysis

523

Amino Acids

59.49

Weight (kDa)

9.3

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 95 - 499 4.1e-81 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 1252
AccB7I CCANNNNNTGG 1 cut(s) 1541
AccIII TCCGGA 1 cut(s) 1054
AcsI RAATTY 1 cut(s) 244
AcuI CTGAAG 2 cut(s) 621, 1362
AfaI GTAC 3 cut(s) 55, 261, 1200
AfiI CCNNNNNNNGG 3 cut(s) 368, 1054, 1541
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 7 cut(s) 275, 338, 1256, 1324, 1439, 1458, 1550
AjnI CCWGG 1 cut(s) 754
AjuI GAANNNNNNNTTGG 6 cut(s) 425, 457, 494, 526, 870, 902
AluBI AGCT 8 cut(s) 303, 439, 525, 554, 672, 717, 805, 885
AluI AGCT 8 cut(s) 303, 439, 525, 554, 672, 717, 805, 885
Alw26I GTCTC 7 cut(s) 17, 441, 513, 557, 816, 1170, 1270
AlwNI CAGNNNCTG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 1054
AoxI GGCC 3 cut(s) 1044, 1123, 1417
ApeKI GCWGC 4 cut(s) 666, 669, 882, 1376
ApoI RAATTY 1 cut(s) 244
ArsI GACNNNNNNTTYG 2 cut(s) 1000, 1032
Asp700I GAANNNNTTC 2 cut(s) 496, 1319
AspS9I GGNCC 5 cut(s) 164, 294, 1045, 1084, 1400
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 3 cut(s) 28, 482, 1474
AvaII GGWCC 4 cut(s) 164, 294, 1084, 1400
BbsI GAAGAC 1 cut(s) 326
BbvI GCAGC 4 cut(s) 678, 681, 894, 1363
BccI CCATC 2 cut(s) 1059, 1115
BciT130I CCWGG 1 cut(s) 756
BclI TGATCA 1 cut(s) 1152
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 7 cut(s) 17, 441, 513, 557, 816, 1170, 1270
BfaI CTAG 5 cut(s) 555, 651, 1274, 1353, 1404
BfmI CTRYAG 2 cut(s) 267, 1088
BfuAI ACCTGC 1 cut(s) 1252
BisI GCNGC 4 cut(s) 667, 670, 883, 1377
BlsI GCNGC 4 cut(s) 668, 671, 884, 1378
Bme1390I CCNGG 2 cut(s) 42, 756
Bme18I GGWCC 4 cut(s) 164, 294, 1084, 1400
BmgT120I GGNCC 5 cut(s) 164, 294, 1045, 1084, 1400
BmiI GGNNCC 2 cut(s) 753, 1401
BmrFI CCNGG 2 cut(s) 42, 756
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 558, 768
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 1322, 1354
BpuEI CTTGAG 2 cut(s) 77, 827
BpuMI CCSGG 1 cut(s) 42
BsaJI CCNNGG 3 cut(s) 184, 459, 514
BsaWI WCCGGW 2 cut(s) 869, 1054
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 1054, 1541
Bse1I ACTGG 3 cut(s) 341, 420, 780
BseAI TCCGGA 1 cut(s) 1054
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 3 cut(s) 184, 459, 514
BseGI GGATG 2 cut(s) 180, 1051
BseLI CCNNNNNNNGG 3 cut(s) 368, 1054, 1541
BseMII CTCAG 8 cut(s) 164, 354, 479, 552, 760, 1160, 1307, 1320
BseNI ACTGG 3 cut(s) 341, 420, 780
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 4 cut(s) 678, 681, 894, 1363
BseYI CCCAGC 1 cut(s) 1492
BshFI GGCC 3 cut(s) 1046, 1125, 1419
BsiSI CCGG 3 cut(s) 42, 870, 1055
BslFI GGGAC 3 cut(s) 904, 1317, 1413
BslI CCNNNNNNNGG 3 cut(s) 368, 1054, 1541
BsmAI GTCTC 7 cut(s) 17, 441, 513, 557, 816, 1170, 1270
BsmBI CGTCTC 1 cut(s) 557
BsmFI GGGAC 3 cut(s) 904, 1317, 1413
BsmI GAATGC 1 cut(s) 1379
BsnI GGCC 3 cut(s) 1046, 1125, 1419
Bsp13I TCCGGA 1 cut(s) 1054
Bsp143I GATC 2 cut(s) 84, 1152
Bsp19I CCATGG 3 cut(s) 184, 459, 514
BspANI GGCC 3 cut(s) 1046, 1125, 1419
BspCNI CTCAG 8 cut(s) 163, 355, 480, 553, 759, 1161, 1306, 1321
BspEI TCCGGA 1 cut(s) 1054
BspHI TCATGA 1 cut(s) 1285
BspLI GGNNCC 2 cut(s) 753, 1401
BspMAI CTGCAG 2 cut(s) 271, 1092
BspMI ACCTGC 1 cut(s) 1252
BsrI ACTGG 3 cut(s) 341, 420, 780
BssECI CCNNGG 3 cut(s) 184, 459, 514
BssMI GATC 2 cut(s) 84, 1152
BssT1I CCWWGG 3 cut(s) 184, 459, 514
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 4 cut(s) 28, 1033, 1207, 1528
Bst6I CTCTTC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 1448
BstC8I GCNNGC 1 cut(s) 922
BstDEI CTNAG 9 cut(s) 150, 363, 488, 561, 746, 1169, 1293, 1329, 1564
BstDSI CCRYGG 3 cut(s) 184, 459, 514
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 2 cut(s) 180, 1051
BstKTI GATC 2 cut(s) 87, 1155
BstMAI GTCTC 7 cut(s) 17, 441, 513, 557, 816, 1170, 1270
BstMBI GATC 2 cut(s) 84, 1152
BstMWI GCNNNNNNNGC 2 cut(s) 926, 1448
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 2 cut(s) 214, 703
BstSCI CCNGG 2 cut(s) 40, 754
BstSFI CTRYAG 2 cut(s) 267, 1088
BstV1I GCAGC 4 cut(s) 678, 681, 894, 1363
BstV2I GAAGAC 1 cut(s) 326
BsuRI GGCC 3 cut(s) 1046, 1125, 1419
BtgI CCRYGG 3 cut(s) 184, 459, 514
BtsCI GGATG 2 cut(s) 180, 1051
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 4 cut(s) 787, 870, 960, 1212
BveI ACCTGC 1 cut(s) 1252
Cac8I GCNNGC 1 cut(s) 922
CaiI CAGNNNCTG 1 cut(s) 362
CciI TCATGA 1 cut(s) 1285
Cfr13I GGNCC 5 cut(s) 164, 294, 1045, 1084, 1400
Csp6I GTAC 3 cut(s) 54, 260, 1199
CviQI GTAC 3 cut(s) 54, 260, 1199
DdeI CTNAG 9 cut(s) 150, 363, 488, 561, 746, 1169, 1293, 1329, 1564
DpnI GATC 2 cut(s) 86, 1154
DpnII GATC 2 cut(s) 84, 1152
DraI TTTAAA 1 cut(s) 915
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 3 cut(s) 184, 459, 514
Eco47I GGWCC 4 cut(s) 164, 294, 1084, 1400
Eco57I CTGAAG 2 cut(s) 621, 1362
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoO109I RGGNCCY 3 cut(s) 164, 1084, 1400
EcoRII CCWGG 1 cut(s) 754
EcoT14I CCWWGG 3 cut(s) 184, 459, 514
ErhI CCWWGG 3 cut(s) 184, 459, 514
Esp3I CGTCTC 1 cut(s) 557
FaqI GGGAC 3 cut(s) 904, 1317, 1413
FbaI TGATCA 1 cut(s) 1152
Fnu4HI GCNGC 4 cut(s) 667, 670, 883, 1377
FokI GGATG 2 cut(s) 167, 1038
Fsp4HI GCNGC 4 cut(s) 667, 670, 883, 1377
FspBI CTAG 5 cut(s) 555, 651, 1274, 1353, 1404
GluI GCNGC 4 cut(s) 667, 670, 883, 1377
GsaI CCCAGC 1 cut(s) 1496
HaeIII GGCC 3 cut(s) 1046, 1125, 1419
HapII CCGG 3 cut(s) 42, 870, 1055
HindIII AAGCTT 1 cut(s) 803
HpaII CCGG 3 cut(s) 42, 870, 1055
HphI GGTGA 3 cut(s) 28, 482, 1474
Hpy166II GTNNAC 5 cut(s) 54, 103, 117, 1504, 1535
Hpy188I TCNGA 6 cut(s) 161, 640, 731, 799, 1170, 1342
Hpy188III TCNNGA 5 cut(s) 94, 542, 1055, 1118, 1286
Hpy8I GTNNAC 5 cut(s) 54, 103, 117, 1504, 1535
HpyCH4III ACNGT 4 cut(s) 28, 1033, 1207, 1528
HpyCH4V TGCA 4 cut(s) 269, 978, 1090, 1379
HpyF10VI GCNNNNNNNGC 2 cut(s) 926, 1448
HpyF3I CTNAG 9 cut(s) 150, 363, 488, 561, 746, 1169, 1293, 1329, 1564
Kpn2I TCCGGA 1 cut(s) 1054
Ksp22I TGATCA 1 cut(s) 1152
Kzo9I GATC 2 cut(s) 84, 1152
Lsp1109I GCAGC 4 cut(s) 678, 681, 894, 1363
LweI GCATC 2 cut(s) 558, 768
MaeI CTAG 5 cut(s) 555, 651, 1274, 1353, 1404
MaeIII GTNAC 1 cut(s) 865
MalI GATC 2 cut(s) 86, 1154
MboI GATC 2 cut(s) 84, 1152
MluCI AATT 6 cut(s) 244, 313, 962, 1223, 1256, 1554
MlyI GAGTC 3 cut(s) 112, 171, 1172
MmeI TCCRAC 2 cut(s) 146, 525
MroI TCCGGA 1 cut(s) 1054
MroXI GAANNNNTTC 2 cut(s) 496, 1319
MseI TTAA 5 cut(s) 527, 713, 843, 914, 1221
MslI CAYNNNNRTG 2 cut(s) 458, 704
MspI CCGG 3 cut(s) 42, 870, 1055
MspR9I CCNGG 2 cut(s) 42, 756
Mva1269I GAATGC 1 cut(s) 1379
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 2 cut(s) 926, 1448
NciI CCSGG 1 cut(s) 42
NcoI CCATGG 3 cut(s) 184, 459, 514
NdeII GATC 2 cut(s) 84, 1152
NlaIV GGNNCC 2 cut(s) 753, 1401
NmuCI GTSAC 1 cut(s) 865
NspI RCATGY 2 cut(s) 214, 703
PagI TCATGA 1 cut(s) 1285
PciI ACATGT 1 cut(s) 699
PctI GAATGC 1 cut(s) 1379
PdmI GAANNNNTTC 2 cut(s) 496, 1319
PfeI GAWTC 7 cut(s) 138, 156, 379, 538, 794, 1335, 1462
PflMI CCANNNNNTGG 1 cut(s) 1541
PkrI GCNGC 4 cut(s) 668, 671, 884, 1378
PleI GAGTC 3 cut(s) 112, 171, 1171
PpsI GAGTC 3 cut(s) 112, 171, 1171
PpuMI RGGWCCY 3 cut(s) 164, 1084, 1400
PscI ACATGT 1 cut(s) 699
PsiI TTATAA 1 cut(s) 774
Psp5II RGGWCCY 3 cut(s) 164, 1084, 1400
Psp6I CCWGG 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 1492
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 753, 1401
PspPI GGNCC 5 cut(s) 164, 294, 1045, 1084, 1400
PspPPI RGGWCCY 3 cut(s) 164, 1084, 1400
PstI CTGCAG 2 cut(s) 271, 1092
PstNI CAGNNNCTG 1 cut(s) 362
RsaI GTAC 3 cut(s) 55, 261, 1200
RsaNI GTAC 3 cut(s) 54, 260, 1199
RseI CAYNNNNRTG 2 cut(s) 458, 704
SaqAI TTAA 5 cut(s) 527, 713, 843, 914, 1221
SatI GCNGC 4 cut(s) 667, 670, 883, 1377
Sau3AI GATC 2 cut(s) 84, 1152
Sau96I GGNCC 5 cut(s) 164, 294, 1045, 1084, 1400
SchI GAGTC 3 cut(s) 112, 171, 1172
ScrFI CCNGG 2 cut(s) 42, 756
SfaNI GCATC 2 cut(s) 558, 768
SfcI CTRYAG 2 cut(s) 267, 1088
SinI GGWCC 4 cut(s) 164, 294, 1084, 1400
SmiMI CAYNNNNRTG 2 cut(s) 458, 704
SmlI CTYRAG 2 cut(s) 92, 806
SmoI CTYRAG 2 cut(s) 92, 806
Sse9I AATT 6 cut(s) 244, 313, 962, 1223, 1256, 1554
SspI AATATT 2 cut(s) 691, 726
SspMI CTAG 5 cut(s) 555, 651, 1274, 1353, 1404
StyD4I CCNGG 2 cut(s) 40, 754
StyI CCWWGG 3 cut(s) 184, 459, 514
TaaI ACNGT 4 cut(s) 28, 1033, 1207, 1528
TaqI TCGA 3 cut(s) 127, 573, 969
TasI AATT 6 cut(s) 244, 313, 962, 1223, 1256, 1554
TatI WGTACW 1 cut(s) 259
TfiI GAWTC 7 cut(s) 138, 156, 379, 538, 794, 1335, 1462
Tru1I TTAA 5 cut(s) 527, 713, 843, 914, 1221
Tru9I TTAA 5 cut(s) 527, 713, 843, 914, 1221
TscAI CASTG 4 cut(s) 787, 870, 960, 1212
TseFI GTSAC 1 cut(s) 865
TseI GCWGC 4 cut(s) 666, 669, 882, 1376
Tsp45I GTSAC 1 cut(s) 865
TspDTI ATGAA 7 cut(s) 130, 606, 630, 722, 975, 1271, 1302
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 4 cut(s) 787, 870, 960, 1212
Van91I CCANNNNNTGG 1 cut(s) 1541
VpaK11BI GGWCC 4 cut(s) 164, 294, 1084, 1400
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 1 cut(s) 244
XceI RCATGY 2 cut(s) 214, 703
XcmI CCANNNNNNNNNTGG 1 cut(s) 512
XmnI GAANNNNTTC 2 cut(s) 496, 1319
XspI CTAG 5 cut(s) 555, 651, 1274, 1353, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.