Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0010533

Physical Location & Seq

Genomic Coordinates Reverse (-)

163878 .. 166651

Gene Structure 2774 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G000110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGGGAGGAGGAGGAGGACTGCGAAAAGCGGCCAAGAAGATGGTGGTGGCAGCTGCCAATGCATGTGGTGGTTCCTTCTCTTCCAGGAGGAAGCCTCTCGTGGACCCCATCGTCTTCTTCGATCATACCACCGCCACTGCCTCTATCTCAGGTTCTTCGGCTGTGTCTCCCACCAACTCCACAGCAGCAGAAGAAGAAGAAGAAGAATGCATTGCAAAGGAGACGGAATCTGCAATCACCAATTCTAACAATGTACCTTCCTCTTCTTCTTCTAATAAGAACTTGTGCGCAATATGTCTAGACCCTCTGAGTTACCATAGCAAGGGACGGATCTCAGGGCAGGCTATATTCACGGCCCAGTGCTCCCATGCATTCCACTTTGCCTGCATCTCATCCAATGTCCGCCATGGTAGTGTCACCTGCCCCATCTGCCGTGCCCATTGGACTCAGCTGCCCCGCAATCTGAACCCACCTGGTGGTTCCCTTTCCTCTTGCAACCCAACTGATCCCATCCTCCAAATCCTTGATGACTCTATAGCCACTTTCCGCATCCACAGGCGCTCTTTCCTGCGCTCTGCTCGCTATGATGATGATGACCCGATTGAGCCTCCCGATCACATGCCTAATTTCCCCCGTCTCCAGTTCTCTCTAGCACCCTTACCACCTAGTACTCCACCCCATAGCTTTCAACCGTCCTGGTGCACCACACACCCATATCATCCACCCCCACATCACCTGAGCTATAGCTCCACATCATTGTTACAATCGCCAACCAGGCCAAAGCCCTATACTACTATGTATGCCTCTTCGGACAGAGCTTATCTTTCGGTGAAATTGGCAGAGCAGCGAGCAACTGATTTTGTCTTGGTTGCTAGTCCCAACGGACCTCACTTGAGGCTTCTCAAGCAGTGCATGGCATTGGTGGTTTTCTCCCTTAGACCTATTGATCGTTTGGCCATTGTTACCTACTCATCAGCTGCTGCTCGTGTCTTCCCTCTAAGACGCATGACATCTTATGGGAAGCGAACAGCCCAGCAGGTTATTGACCGCCTGTTCTACATGGGGCAAGCAGATCCAGTGGAAGGGCTCAAGAAAGGGATCAAGATACTTCAAGATCGTGTATACAAGAATCAAAATTCGGGTATCCTGCATCTTTCTGACACCCCAACTCGATCCTACCATGCTGCTCTCAATATGGACGTGCCCATTCCAGTGCATCGTTTTCATGTAGGATTTGGATTTGGCACTTCAAATGGTTTTATCATGCATGAATTTGAAGAGTTCCTCAGAAGACTCATAGGTGGTGTTGTTAGAGAAATCCAGTTAAGGATCAGCACAACTGAGGAAGCCAGCAGTAGGATTGTAAGAATAGGAGAGCTAAGAAACGGAGAAGAAAGGAAAATCTTAGTGGACTTGAGTGTTTGTGGACATATTTGCGTGGGATACAGCTACATAGAGGATGGTGAGATTGACGAACCCATCACAACAGGGGAAACTATGGTGAGCATAGGAGAGGGTAAAAGTAATATGAGTGCAGCTGAAGCAGCGTCTGCTGGGTCAGGGACGAGCGGTCCAATTATTGGTGGGAGGCCCAGTAGTGCAGATAGTTGGGACTACCATGATCCTTACATGGCTAGAAGATGGGCCAAACATTTGCATGGCTACAGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA_2 zf-C3HC4 zf-RING_2 zf-rbx1

Protein Analysis

557

Amino Acids

61.01

Weight (kDa)

8.6

Isoelectric Point (pI)

58.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	95 - 145	2e-07	Ring finger domain
zf-rbx1	PF12678	95 - 145	1.3e-06	RING-H2 zinc finger domain
zf-C3HC4	PF00097	96 - 144	1.9e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	96 - 130	1.8e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00090 FvH4_3g00090
malus_domestica	MD05G1364100.v1.1
prunus_persica	Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1
pyrus_communis	pycom05g33030
rosa_chinensis	RchiOBHm_Chr5g0000121
rosa_laevigata	RLG00000030843
rosa_multiflora	Rmu_sc0003125.1_g000002 Rmu_ssc0000243.1_g000019
rosa_roxburghii	Rroxscaffold_1G00075980
rosa_rugosa	Rorug04G0381700
rosa_samantha	Rh5AG000800 Rh5BG002000 Rh5CG000900 Rh5DG000800
rosa_wichuraiana	Rw5G000110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	428
AasI	GACNNNNNNGTC	1 cut(s)	110
Acc16I	TGCGCA	1 cut(s)	289
Acc36I	ACCTGC	2 cut(s)	428, 1027
AccB7I	CCANNNNNTGG	2 cut(s)	476, 1580
AccBSI	CCGCTC	1 cut(s)	1569
AccI	GTMKAC	1 cut(s)	1122
AciI	CCGC	7 cut(s)	29, 132, 403, 457, 547, 1048, 1569
AclWI	GGATC	7 cut(s)	338, 500, 1067, 1106, 1167, 1337, 1616
AcoI	YGGCCR	2 cut(s)	30, 954
AcsI	RAATTY	2 cut(s)	1135, 1271
AcuI	CTGAAG	1 cut(s)	1560
AdeI	CACNNNGTG	1 cut(s)	476
AfaI	GTAC	2 cut(s)	255, 670
AfiI	CCNNNNNNNGG	5 cut(s)	322, 476, 1082, 1356, 1580
AgsI	TTSAA	4 cut(s)	689, 1112, 1251, 1277
AhdI	GACNNNNNGTC	1 cut(s)	1569
AjiI	CACGTC	1 cut(s)	1201
AjnI	CCWGG	4 cut(s)	83, 472, 695, 773
Alw21I	GWGCWC	2 cut(s)	365, 704
Alw26I	GTCTC	3 cut(s)	171, 215, 641
Alw44I	GTGCAC	1 cut(s)	700
AlwI	GGATC	7 cut(s)	338, 500, 1067, 1106, 1167, 1337, 1616
AlwNI	CAGNNNCTG	2 cut(s)	980, 1550
AoxI	GGCC	6 cut(s)	30, 354, 776, 954, 1589, 1644
ApaLI	GTGCAC	1 cut(s)	700
ApoI	RAATTY	2 cut(s)	1135, 1271
AspLEI	GCGC	3 cut(s)	290, 561, 573
AspS9I	GGNCC	6 cut(s)	103, 355, 884, 1571, 1590, 1644
AsuHPI	GGTGA	6 cut(s)	229, 409, 725, 841, 1475, 1513
AvaII	GGWCC	3 cut(s)	103, 884, 1571
BaeGI	GKGCMC	3 cut(s)	439, 704, 1206
BalI	TGGCCA	1 cut(s)	956
BanII	GRGCYC	1 cut(s)	1089
BauI	CACGAG	2 cut(s)	98, 984
BbsI	GAAGAC	3 cut(s)	106, 982, 1297
Bbv12I	GWGCWC	2 cut(s)	365, 704
BccI	CCATC	7 cut(s)	34, 116, 434, 518, 1454, 1487, 1635
BceAI	ACGGC	2 cut(s)	369, 417
BciT130I	CCWGG	4 cut(s)	85, 474, 697, 775
BciVI	GTATCC	2 cut(s)	1154, 1436
BcoDI	GTCTC	3 cut(s)	171, 215, 641
BfaI	CTAG	5 cut(s)	299, 650, 666, 873, 1635
BfmI	CTRYAG	3 cut(s)	534, 742, 1663
BfoI	RGCGCY	1 cut(s)	562
BfuAI	ACCTGC	2 cut(s)	428, 1027
BfuI	GTATCC	2 cut(s)	1154, 1436
BglI	GCCNNNNNGGC	1 cut(s)	775
BmcAI	AGTACT	1 cut(s)	670
Bme1390I	CCNGG	4 cut(s)	85, 474, 697, 775
Bme18I	GGWCC	3 cut(s)	103, 884, 1571
BmeRI	GACNNNNNGTC	1 cut(s)	1569
BmgBI	CACGTC	1 cut(s)	1201
BmgT120I	GGNCC	6 cut(s)	103, 355, 884, 1571, 1590, 1644
BmiI	GGNNCC	3 cut(s)	73, 105, 481
BmrFI	CCNGG	4 cut(s)	85, 474, 697, 775
BmrI	ACTGGG	2 cut(s)	352, 1587
BmsI	GCATC	4 cut(s)	396, 558, 1159, 1225
BmuI	ACTGGG	2 cut(s)	352, 1587
BpiI	GAAGAC	3 cut(s)	106, 982, 1297
BplI	GAGNNNNNCTC	2 cut(s)	79, 111
BpmI	CTGGAG	1 cut(s)	623
Bpu10I	CCTNAGC	1 cut(s)	737
BpuEI	CTTGAG	4 cut(s)	887, 913, 1073, 1435
BsaJI	CCNNGG	1 cut(s)	406
BsaXI	ACNNNNNCTCC	2 cut(s)	1579, 1609
Bsc4I	CCNNNNNNNGG	5 cut(s)	322, 476, 1082, 1356, 1580
Bse1I	ACTGG	6 cut(s)	358, 640, 1076, 1211, 1321, 1593
Bse3DI	GCAATG	1 cut(s)	210
BseBI	CCWGG	4 cut(s)	85, 474, 697, 775
BseDI	CCNNGG	1 cut(s)	406
BseGI	GGATG	5 cut(s)	392, 510, 549, 718, 1465
BseLI	CCNNNNNNNGG	5 cut(s)	322, 476, 1082, 1356, 1580
BseMI	GCAATG	1 cut(s)	210
BseMII	CTCAG	7 cut(s)	162, 299, 348, 461, 728, 1300, 1332
BseNI	ACTGG	6 cut(s)	358, 640, 1076, 1211, 1321, 1593
BseRI	GAGGAG	3 cut(s)	21, 24, 27
BseSI	GKGCMC	3 cut(s)	439, 704, 1206
BseYI	CCCAGC	2 cut(s)	1032, 1553
BsgI	GTGCAG	2 cut(s)	1554, 1620
BshFI	GGCC	6 cut(s)	32, 356, 778, 956, 1591, 1646
BsiHKAI	GWGCWC	2 cut(s)	365, 704
BslFI	GGGAC	4 cut(s)	339, 861, 1576, 1625
BslI	CCNNNNNNNGG	5 cut(s)	322, 476, 1082, 1356, 1580
BsmAI	GTCTC	3 cut(s)	171, 215, 641
BsmBI	CGTCTC	2 cut(s)	215, 641
BsmFI	GGGAC	4 cut(s)	339, 861, 1576, 1625
BsmI	GAATGC	2 cut(s)	212, 371
BsnI	GGCC	6 cut(s)	32, 356, 778, 956, 1591, 1646
Bsp1286I	GDGCHC	5 cut(s)	365, 439, 704, 1089, 1206
Bsp19I	CCATGG	1 cut(s)	406
BspACI	CCGC	7 cut(s)	29, 132, 403, 457, 547, 1048, 1569
BspANI	GGCC	6 cut(s)	32, 356, 778, 956, 1591, 1646
BspCNI	CTCAG	7 cut(s)	161, 300, 347, 460, 729, 1299, 1333
BspLI	GGNNCC	3 cut(s)	73, 105, 481
BspMI	ACCTGC	2 cut(s)	428, 1027
BspPI	GGATC	7 cut(s)	338, 500, 1067, 1106, 1167, 1337, 1616
BsrBI	CCGCTC	1 cut(s)	1569
BsrDI	GCAATG	1 cut(s)	210
BsrI	ACTGG	6 cut(s)	358, 640, 1076, 1211, 1321, 1593
BssECI	CCNNGG	1 cut(s)	406
BssNAI	GTATAC	1 cut(s)	1123
BssSI	CACGAG	2 cut(s)	98, 984
BssT1I	CCWWGG	1 cut(s)	406
Bst1107I	GTATAC	1 cut(s)	1123
Bst2BI	CACGAG	2 cut(s)	98, 984
Bst2UI	CCWGG	4 cut(s)	85, 474, 697, 775
Bst4CI	ACNGT	1 cut(s)	693
Bst6I	CTCTTC	4 cut(s)	85, 268, 811, 1272
BstAPI	GCANNNNNTGC	1 cut(s)	1550
BstC8I	GCNNGC	6 cut(s)	342, 385, 580, 849, 1068, 1351
BstDSI	CCRYGG	1 cut(s)	406
BstF5I	GGATG	5 cut(s)	392, 510, 549, 718, 1465
BstH2I	RGCGCY	1 cut(s)	562
BstHHI	GCGC	3 cut(s)	290, 561, 573
BstMAI	GTCTC	3 cut(s)	171, 215, 641
BstMWI	GCNNNNNNNGC	8 cut(s)	59, 429, 579, 775, 904, 1541, 1544, 1550
BstNI	CCWGG	4 cut(s)	85, 474, 697, 775
BstNSI	RCATGY	2 cut(s)	66, 622
BstSCI	CCNGG	4 cut(s)	83, 472, 695, 773
BstSFI	CTRYAG	3 cut(s)	534, 742, 1663
BstSLI	GKGCMC	3 cut(s)	439, 704, 1206
BstV2I	GAAGAC	3 cut(s)	106, 982, 1297
BstX2I	RGATCY	2 cut(s)	330, 1072
BstXI	CCANNNNNNTGG	1 cut(s)	40
BstYI	RGATCY	2 cut(s)	330, 1072
BstZ17I	GTATAC	1 cut(s)	1123
BsuI	GTATCC	2 cut(s)	1154, 1436
BsuRI	GGCC	6 cut(s)	32, 356, 778, 956, 1591, 1646
BtgI	CCRYGG	1 cut(s)	406
BtrI	CACGTC	1 cut(s)	1201
BtsCI	GGATG	5 cut(s)	392, 510, 549, 718, 1465
BtsI	GCAGTG	2 cut(s)	135, 914
BtsIMutI	CAGTG	5 cut(s)	135, 365, 914, 1083, 1218
BveI	ACCTGC	2 cut(s)	428, 1027
Cac8I	GCNNGC	6 cut(s)	342, 385, 580, 849, 1068, 1351
CaiI	CAGNNNCTG	2 cut(s)	980, 1550
CfoI	GCGC	3 cut(s)	290, 561, 573
Cfr13I	GGNCC	6 cut(s)	103, 355, 884, 1571, 1590, 1644
CseI	GACGC	2 cut(s)	1011, 1536
CsiI	ACCWGGT	1 cut(s)	472
Csp6I	GTAC	2 cut(s)	254, 669
CspCI	CAANNNNNGTGG	2 cut(s)	46, 81
CviQI	GTAC	2 cut(s)	254, 669
DraIII	CACNNNGTG	1 cut(s)	476
DrdI	GACNNNNNNGTC	1 cut(s)	110
DriI	GACNNNNNGTC	1 cut(s)	1569
DseDI	GACNNNNNNGTC	1 cut(s)	110
EaeI	YGGCCR	2 cut(s)	30, 954
Eam1104I	CTCTTC	4 cut(s)	85, 268, 811, 1272
Eam1105I	GACNNNNNGTC	1 cut(s)	1569
EarI	CTCTTC	4 cut(s)	85, 268, 811, 1272
EciI	GGCGGA	1 cut(s)	392
Eco130I	CCWWGG	1 cut(s)	406
Eco24I	GRGCYC	1 cut(s)	1089
Eco47I	GGWCC	3 cut(s)	103, 884, 1571
Eco57I	CTGAAG	1 cut(s)	1560
EcoRII	CCWGG	4 cut(s)	83, 472, 695, 773
EcoT14I	CCWWGG	1 cut(s)	406
EcoT22I	ATGCAT	4 cut(s)	64, 212, 373, 1269
EcoT38I	GRGCYC	1 cut(s)	1089
ErhI	CCWWGG	1 cut(s)	406
Esp3I	CGTCTC	2 cut(s)	215, 641
FaqI	GGGAC	4 cut(s)	339, 861, 1576, 1625
FauI	CCCGC	1 cut(s)	464
FblI	GTMKAC	1 cut(s)	1122
FokI	GGATG	5 cut(s)	379, 497, 536, 705, 1472
FriOI	GRGCYC	1 cut(s)	1089
FspBI	CTAG	5 cut(s)	299, 650, 666, 873, 1635
FspI	TGCGCA	1 cut(s)	289
GlaI	GCGC	3 cut(s)	289, 560, 572
GsaI	CCCAGC	2 cut(s)	1036, 1557
GsuI	CTGGAG	1 cut(s)	623
HaeII	RGCGCY	1 cut(s)	562
HaeIII	GGCC	6 cut(s)	32, 356, 778, 956, 1591, 1646
HgaI	GACGC	2 cut(s)	1011, 1536
HhaI	GCGC	3 cut(s)	290, 561, 573
Hin6I	GCGC	3 cut(s)	288, 559, 571
HinP1I	GCGC	3 cut(s)	288, 559, 571
HinfI	GANTC	5 cut(s)	227, 445, 530, 1129, 1293
HphI	GGTGA	6 cut(s)	229, 409, 725, 841, 1475, 1513
Hpy166II	GTNNAC	5 cut(s)	103, 702, 1123, 1411, 1427
Hpy188I	TCNGA	5 cut(s)	309, 465, 811, 1159, 1289
Hpy188III	TCNNGA	5 cut(s)	299, 611, 1090, 1102, 1112
Hpy8I	GTNNAC	5 cut(s)	103, 702, 1123, 1411, 1427
HpyAV	CCTTC	3 cut(s)	85, 267, 1076
HpyCH4III	ACNGT	1 cut(s)	693
HpyCH4IV	ACGT	1 cut(s)	1200
HpyF10VI	GCNNNNNNNGC	8 cut(s)	59, 429, 579, 775, 904, 1541, 1544, 1550
HpySE526I	ACGT	1 cut(s)	1200
HspAI	GCGC	3 cut(s)	288, 559, 571
LmnI	GCTCC	2 cut(s)	368, 752
LweI	GCATC	4 cut(s)	396, 558, 1159, 1225
MabI	ACCWGGT	1 cut(s)	472
MaeI	CTAG	5 cut(s)	299, 650, 666, 873, 1635
MaeII	ACGT	1 cut(s)	1200
MaeIII	GTNAC	4 cut(s)	311, 415, 759, 961
MbiI	CCGCTC	1 cut(s)	1569
MflI	RGATCY	2 cut(s)	330, 1072
MhlI	GDGCHC	5 cut(s)	365, 439, 704, 1089, 1206
MlsI	TGGCCA	1 cut(s)	956
MluCI	AATT	6 cut(s)	241, 625, 833, 1135, 1271, 1575
MluNI	TGGCCA	1 cut(s)	956
MlyI	GAGTC	3 cut(s)	439, 524, 1287
Mox20I	TGGCCA	1 cut(s)	956
Mph1103I	ATGCAT	4 cut(s)	64, 212, 373, 1269
MscI	TGGCCA	1 cut(s)	956
MseI	TTAA	1 cut(s)	1325
MslI	CAYNNNNRTG	3 cut(s)	411, 1211, 1656
Msp20I	TGGCCA	1 cut(s)	956
MspA1I	CMGCKG	4 cut(s)	53, 451, 977, 1538
MspR9I	CCNGG	4 cut(s)	85, 474, 697, 775
Mva1269I	GAATGC	2 cut(s)	212, 371
MvaI	CCWGG	4 cut(s)	85, 474, 697, 775
MwoI	GCNNNNNNNGC	8 cut(s)	59, 429, 579, 775, 904, 1541, 1544, 1550
NcoI	CCATGG	1 cut(s)	406
NlaIV	GGNNCC	3 cut(s)	73, 105, 481
NmuCI	GTSAC	1 cut(s)	415
NsbI	TGCGCA	1 cut(s)	289
NsiI	ATGCAT	4 cut(s)	64, 212, 373, 1269
NspI	RCATGY	2 cut(s)	66, 622
PaqCI	CACCTGC	1 cut(s)	428
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	2 cut(s)	212, 371
PfeI	GAWTC	2 cut(s)	227, 1129
PflMI	CCANNNNNTGG	2 cut(s)	476, 1580
PfoI	TCCNGGA	1 cut(s)	83
PleI	GAGTC	3 cut(s)	439, 524, 1287
PpsI	GAGTC	3 cut(s)	439, 524, 1287
Psp6I	CCWGG	4 cut(s)	83, 472, 695, 773
PspFI	CCCAGC	2 cut(s)	1032, 1553
PspGI	CCWGG	4 cut(s)	83, 472, 695, 773
PspN4I	GGNNCC	3 cut(s)	73, 105, 481
PspPI	GGNCC	6 cut(s)	103, 355, 884, 1571, 1590, 1644
PstNI	CAGNNNCTG	2 cut(s)	980, 1550
PsuI	RGATCY	2 cut(s)	330, 1072
PvuII	CAGCTG	4 cut(s)	53, 451, 977, 1538
RsaI	GTAC	2 cut(s)	255, 670
RsaNI	GTAC	2 cut(s)	254, 669
RseI	CAYNNNNRTG	3 cut(s)	411, 1211, 1656
SaqAI	TTAA	1 cut(s)	1325
Sau96I	GGNCC	6 cut(s)	103, 355, 884, 1571, 1590, 1644
ScaI	AGTACT	1 cut(s)	670
SchI	GAGTC	3 cut(s)	439, 524, 1287
ScrFI	CCNGG	4 cut(s)	85, 474, 697, 775
SduI	GDGCHC	5 cut(s)	365, 439, 704, 1089, 1206
SexAI	ACCWGGT	1 cut(s)	472
SfaNI	GCATC	4 cut(s)	396, 558, 1159, 1225
SfcI	CTRYAG	3 cut(s)	534, 742, 1663
SinI	GGWCC	3 cut(s)	103, 884, 1571
SmiMI	CAYNNNNRTG	3 cut(s)	411, 1211, 1656
SmlI	CTYRAG	4 cut(s)	892, 902, 1088, 1414
SmoI	CTYRAG	4 cut(s)	892, 902, 1088, 1414
Sse9I	AATT	6 cut(s)	241, 625, 833, 1135, 1271, 1575
SsiI	CCGC	7 cut(s)	29, 132, 403, 457, 547, 1048, 1569
SspMI	CTAG	5 cut(s)	299, 650, 666, 873, 1635
StyD4I	CCNGG	4 cut(s)	83, 472, 695, 773
StyI	CCWWGG	1 cut(s)	406
TaaI	ACNGT	1 cut(s)	693
TaiI	ACGT	1 cut(s)	1203
TaqI	TCGA	2 cut(s)	120, 1171
TasI	AATT	6 cut(s)	241, 625, 833, 1135, 1271, 1575
TatI	WGTACW	1 cut(s)	668
TauI	GCSGC	1 cut(s)	32
TfiI	GAWTC	2 cut(s)	227, 1129
Tru1I	TTAA	1 cut(s)	1325
Tru9I	TTAA	1 cut(s)	1325
TscAI	CASTG	5 cut(s)	142, 365, 914, 1083, 1218
TseFI	GTSAC	1 cut(s)	415
Tsp45I	GTSAC	1 cut(s)	415
TspDTI	ATGAA	2 cut(s)	1214, 1284
TspGWI	ACGGA	4 cut(s)	239, 343, 897, 1401
TspRI	CASTG	5 cut(s)	142, 365, 914, 1083, 1218
Van91I	CCANNNNNTGG	2 cut(s)	476, 1580
VneI	GTGCAC	1 cut(s)	700
VpaK11BI	GGWCC	3 cut(s)	103, 884, 1571
XapI	RAATTY	2 cut(s)	1135, 1271
XbaI	TCTAGA	1 cut(s)	298
XceI	RCATGY	2 cut(s)	66, 622
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XmiI	GTMKAC	1 cut(s)	1122
XspI	CTAG	5 cut(s)	299, 650, 666, 873, 1635
ZrmI	AGTACT	1 cut(s)	670
Zsp2I	ATGCAT	4 cut(s)	64, 212, 373, 1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.