Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp04

Orthogroup ID

OG0010533

Physical Location & Seq

Genomic Coordinates Reverse (-)

122482 .. 126470

Gene Structure 3989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.4G001000.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGGAATGGGAGGAGCAGGAGGAGGAGCATCGTCAAAATCTAAACTGAGAAATGCAGCGAAGAAAATGGTGGTGGCAGCTGCAAATGCTTGTGGTTCCTTCTCCCGGAGAAAGGCTCTTGTGGATCCCATCGTCTTCGATCATACCAACACCACAGCCACTGCCTCTATCTCAGGTTCTTCAGCGGTGTCTCCCACAACCCCAAAGAATATCTCAGGAGAAGAAGTTGCAAAGGAAACGGAATTTACGATCAAGAGTAACAATATGCCTTCAACTTCAACTAAGAATTTGTGCGCAATATGTCTAGACCCTCTGAGTTACCATAGCAAGAGCAGCACCCCAGGTCTTGCTATATTCACGGCCCAGTGCTCCCATGCTTTCCACTTTGCCTGCATCTCCTCCAATGTCCGCCATGGTAGTGTTACCTGCCCCATCTGCCGTGCCCATTGGACTCAGCTGCCTCGCAATCTGAACCCACCCGGGGGTTCCCTCTCGTCTTGCAACCGACCTGACCCCATCCTCCAAATCCTTGATGACTCTATTGCCACTTTCCGTATCCACAGGCGCTCCTTCTTGCGCTCTGCTCACTATGATGATGATGACCCAATTGAGCCTGATCATATGCCTAATTGGCCTCGTCTCCAACTCTCTCTAATACCCATCCCACCTAGTGCACCCCCTAGCTGGTGCACCCCATACCCATACCATCCGTCCCCACATCACCAAAGCTGTAGCTCTTCATCATTGTTACAATCACCAACTAGGCCAAAGCCTTTTACTCTCTGTGCCTTTTCAGATAGGGCATATCTTTCCGTGAAATTAGCACATCAACGAGCAACAGACTTGGTCTTGGTTGCTAGCCCCAATGGGCCACACTTGAGGCTTCTCAAGCAGTGCATGGCACTGGTGGTTTTTTCTCTTAGACCTATTGACCGTTTGGCCATTGTTACCTACTCATCAGCTGCTGCCCGTCTCTTCCCTTTGAGACGCATGACATCTTATGGGAAGCGAACGGCCCAACAGGTTATTGACCGTCTGTTCTATATGGGGCAAGCAGATCCAATAGAAGGGATCAAGAAAGGAATCAAGATACTTGAAGATCGTGCATACAAGAATCCAGAGTCCAGTATCCTGCATCTGTCTGACAGCCCAACCCAATCCTACCATGCTGCAATGAGCATGGAGTTGCCGATTCCAGTCCATCGCTTTCATGTAGGATTTGGCTTTGGCACTTCAAATGGGTTTATCATGCATGAGTTTGAAGAGTTACTAGGAACACTCATAGGTGGCATCGTTAGAGAAGTCCAGTTGAGAATCAGAATAGGGGAGGAGGCAAGCAGTAGGATCGTAAGGATAGGAGAGCTAAGAGGAGGTGAAGAAAGGAAAATCTTGGTGGAATTGGGTGTGTGTGGACATATCTGTGTGGGATATAGTTATATTGAGGAGGGTGAGATTGACGAACCTTTCACAACAGGAGAAACTGTGGTGAGCATAGGAGACAGCAAAAGTAAGGCAACAGAAGGAGCGGCTGCTGCTTCTGGGACGAGCGATGCAATCATTTGTGGGAGGTCTAGCAGTGCAAGTGTAGAAACTTGGGATTACCATGATCCTTACATGGCTAGAAGATGGGCCAAGCATTTGCATGGCTACAGGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA_2 zf-C3HC4 zf-RING_2 zf-rbx1

Protein Analysis

553

Amino Acids

60.21

Weight (kDa)

8.8

Isoelectric Point (pI)

57.47

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00090 FvH4_3g00090
malus_domestica	MD05G1364100.v1.1
prunus_persica	Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1 Prupe.4G001000_v2.0.a1
pyrus_communis	pycom05g33030
rosa_chinensis	RchiOBHm_Chr5g0000121
rosa_laevigata	RLG00000030843
rosa_multiflora	Rmu_sc0003125.1_g000002 Rmu_ssc0000243.1_g000019
rosa_roxburghii	Rroxscaffold_1G00075980
rosa_rugosa	Rorug04G0381700
rosa_samantha	Rh5AG000800 Rh5BG002000 Rh5CG000900 Rh5DG000800
rosa_wichuraiana	Rw5G000110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	295
Acc36I	ACCTGC	1 cut(s)	434
AccBSI	CCGCTC	1 cut(s)	1526
AciI	CCGC	3 cut(s)	185, 409, 1526
AclWI	GGATC	6 cut(s)	119, 132, 1052, 1079, 1352, 1601
AcoI	YGGCCR	1 cut(s)	939
AcsI	RAATTY	2 cut(s)	242, 286
AcuI	CTGAAG	1 cut(s)	165
AdeI	CACNNNGTG	1 cut(s)	671
AfiI	CCNNNNNNNGG	6 cut(s)	105, 112, 481, 482, 684, 1067
AgsI	TTSAA	5 cut(s)	273, 279, 1097, 1236, 1262
AjnI	CCWGG	1 cut(s)	340
AluBI	AGCT	7 cut(s)	80, 457, 684, 729, 735, 962, 1363
AluI	AGCT	7 cut(s)	80, 457, 684, 729, 735, 962, 1363
Alw21I	GWGCWC	3 cut(s)	371, 676, 692
Alw26I	GTCTC	5 cut(s)	195, 644, 977, 979, 1491
Alw44I	GTGCAC	2 cut(s)	672, 688
AlwI	GGATC	6 cut(s)	119, 132, 1052, 1079, 1352, 1601
AlwNI	CAGNNNCTG	3 cut(s)	161, 965, 1656
Ama87I	CYCGRG	1 cut(s)	479
AoxI	GGCC	7 cut(s)	360, 632, 764, 869, 939, 1014, 1629
ApaLI	GTGCAC	2 cut(s)	672, 688
ApoI	RAATTY	2 cut(s)	242, 286
AspLEI	GCGC	3 cut(s)	296, 567, 579
AspS9I	GGNCC	4 cut(s)	361, 869, 1015, 1629
AsuC2I	CCSGG	3 cut(s)	106, 480, 481
AsuHPI	GGTGA	5 cut(s)	713, 747, 1385, 1460, 1498
AsuNHI	GCTAGC	1 cut(s)	857
AvaI	CYCGRG	1 cut(s)	479
BaeGI	GKGCMC	3 cut(s)	445, 676, 692
BalI	TGGCCA	1 cut(s)	941
BamHI	GGATCC	1 cut(s)	124
BbsI	GAAGAC	1 cut(s)	127
Bbv12I	GWGCWC	3 cut(s)	371, 676, 692
BccI	CCATC	7 cut(s)	137, 440, 524, 668, 714, 1209, 1620
BceAI	ACGGC	3 cut(s)	375, 423, 1029
BcgI	CGANNNNNNTGC	2 cut(s)	1327, 1361
BciT130I	CCWGG	1 cut(s)	342
BciVI	GTATCC	2 cut(s)	566, 1139
BclI	TGATCA	1 cut(s)	616
BcnI	CCSGG	3 cut(s)	106, 480, 481
BcoDI	GTCTC	5 cut(s)	195, 644, 977, 979, 1491
BfaI	CTAG	8 cut(s)	305, 669, 681, 762, 858, 1271, 1572, 1620
BfmI	CTRYAG	2 cut(s)	730, 1648
BfoI	RGCGCY	1 cut(s)	568
BfuAI	ACCTGC	1 cut(s)	434
BfuI	GTATCC	2 cut(s)	566, 1139
BglI	GCCNNNNNGGC	1 cut(s)	631
Bme1390I	CCNGG	4 cut(s)	106, 342, 480, 481
BmeT110I	CYCGRG	1 cut(s)	479
BmgT120I	GGNCC	4 cut(s)	361, 869, 1015, 1629
BmiI	GGNNCC	3 cut(s)	97, 126, 487
BmrFI	CCNGG	4 cut(s)	106, 342, 480, 481
BmrI	ACTGGG	1 cut(s)	358
BmsI	GCATC	5 cut(s)	38, 402, 1144, 1299, 1540
BmtI	GCTAGC	1 cut(s)	861
BmuI	ACTGGG	1 cut(s)	358
BpiI	GAAGAC	1 cut(s)	127
BplI	GAGNNNNNCTC	2 cut(s)	100, 132
BpuEI	CTTGAG	2 cut(s)	872, 898
BpuMI	CCSGG	3 cut(s)	106, 480, 481
BsaJI	CCNNGG	4 cut(s)	340, 412, 479, 480
BsaXI	ACNNNNNCTCC	6 cut(s)	210, 240, 551, 581, 1467, 1497
Bsc4I	CCNNNNNNNGG	6 cut(s)	105, 112, 481, 482, 684, 1067
Bse1I	ACTGG	5 cut(s)	364, 909, 1125, 1196, 1306
Bse3DI	GCAATG	1 cut(s)	1179
BseBI	CCWGG	1 cut(s)	342
BseDI	CCNNGG	4 cut(s)	340, 412, 479, 480
BseGI	GGATG	3 cut(s)	516, 660, 706
BseLI	CCNNNNNNNGG	6 cut(s)	105, 112, 481, 482, 684, 1067
BseMI	GCAATG	1 cut(s)	1179
BseMII	CTCAG	5 cut(s)	38, 186, 228, 305, 467
BseNI	ACTGG	5 cut(s)	364, 909, 1125, 1196, 1306
BseRI	GAGGAG	7 cut(s)	27, 36, 39, 388, 1343, 1383, 1457
BseSI	GKGCMC	3 cut(s)	445, 676, 692
BshFI	GGCC	7 cut(s)	362, 634, 766, 871, 941, 1016, 1631
BsiHKAI	GWGCWC	3 cut(s)	371, 676, 692
BsiHKCI	CYCGRG	1 cut(s)	479
BsiSI	CCGG	2 cut(s)	106, 480
BslFI	GGGAC	2 cut(s)	697, 1555
BslI	CCNNNNNNNGG	6 cut(s)	105, 112, 481, 482, 684, 1067
BsmAI	GTCTC	5 cut(s)	195, 644, 977, 979, 1491
BsmBI	CGTCTC	3 cut(s)	644, 977, 979
BsmFI	GGGAC	2 cut(s)	697, 1555
BsnI	GGCC	7 cut(s)	362, 634, 766, 871, 941, 1016, 1631
BsoBI	CYCGRG	1 cut(s)	479
Bsp1286I	GDGCHC	4 cut(s)	371, 445, 676, 692
Bsp143I	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
Bsp19I	CCATGG	1 cut(s)	412
BspACI	CCGC	3 cut(s)	185, 409, 1526
BspANI	GGCC	7 cut(s)	362, 634, 766, 871, 941, 1016, 1631
BspCNI	CTCAG	5 cut(s)	39, 185, 227, 306, 466
BspLI	GGNNCC	3 cut(s)	97, 126, 487
BspMI	ACCTGC	1 cut(s)	434
BspOI	GCTAGC	1 cut(s)	861
BspPI	GGATC	6 cut(s)	119, 132, 1052, 1079, 1352, 1601
BspQI	GCTCTTC	1 cut(s)	742
BsrBI	CCGCTC	1 cut(s)	1526
BsrDI	GCAATG	1 cut(s)	1179
BsrI	ACTGG	5 cut(s)	364, 909, 1125, 1196, 1306
BssECI	CCNNGG	4 cut(s)	340, 412, 479, 480
BssMI	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
BssT1I	CCWWGG	1 cut(s)	412
Bst2UI	CCWGG	1 cut(s)	342
Bst4CI	ACNGT	3 cut(s)	935, 1034, 1483
Bst6I	CTCTTC	3 cut(s)	742, 980, 1257
BstC8I	GCNNGC	4 cut(s)	391, 859, 1053, 1336
BstDEI	CTNAG	8 cut(s)	47, 172, 214, 282, 314, 453, 920, 1364
BstDSI	CCRYGG	1 cut(s)	412
BstF5I	GGATG	3 cut(s)	516, 660, 706
BstH2I	RGCGCY	1 cut(s)	568
BstHHI	GCGC	3 cut(s)	296, 567, 579
BstKTI	GATC	9 cut(s)	127, 142, 252, 619, 1060, 1074, 1102, 1347, 1609
BstMAI	GTCTC	5 cut(s)	195, 644, 977, 979, 1491
BstMBI	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
BstMWI	GCNNNNNNNGC	6 cut(s)	86, 333, 435, 631, 889, 1532
BstNI	CCWGG	1 cut(s)	342
BstSCI	CCNGG	4 cut(s)	104, 340, 478, 479
BstSFI	CTRYAG	2 cut(s)	730, 1648
BstSLI	GKGCMC	3 cut(s)	445, 676, 692
BstV2I	GAAGAC	1 cut(s)	127
BstX2I	RGATCY	2 cut(s)	124, 1057
BstYI	RGATCY	2 cut(s)	124, 1057
BsuI	GTATCC	2 cut(s)	566, 1139
BsuRI	GGCC	7 cut(s)	362, 634, 766, 871, 941, 1016, 1631
BtgI	CCRYGG	1 cut(s)	412
BtgZI	GCGATG	2 cut(s)	1187, 1563
BtsCI	GGATG	3 cut(s)	516, 660, 706
BtsI	GCAGTG	3 cut(s)	159, 899, 1582
BtsIMutI	CAGTG	5 cut(s)	159, 371, 899, 902, 1582
BveI	ACCTGC	1 cut(s)	434
Cac8I	GCNNGC	4 cut(s)	391, 859, 1053, 1336
CaiI	CAGNNNCTG	3 cut(s)	161, 965, 1656
CfoI	GCGC	3 cut(s)	296, 567, 579
Cfr13I	GGNCC	4 cut(s)	361, 869, 1015, 1629
Cfr9I	CCCGGG	1 cut(s)	479
CseI	GACGC	1 cut(s)	996
DdeI	CTNAG	8 cut(s)	47, 172, 214, 282, 314, 453, 920, 1364
DpnI	GATC	9 cut(s)	126, 141, 251, 618, 1059, 1073, 1101, 1346, 1608
DpnII	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
DraIII	CACNNNGTG	1 cut(s)	671
EaeI	YGGCCR	1 cut(s)	939
Eam1104I	CTCTTC	3 cut(s)	742, 980, 1257
EarI	CTCTTC	3 cut(s)	742, 980, 1257
EciI	GGCGGA	1 cut(s)	398
Eco130I	CCWWGG	1 cut(s)	412
Eco57I	CTGAAG	1 cut(s)	165
Eco88I	CYCGRG	1 cut(s)	479
EcoRII	CCWGG	1 cut(s)	340
EcoT14I	CCWWGG	1 cut(s)	412
EcoT22I	ATGCAT	1 cut(s)	1254
ErhI	CCWWGG	1 cut(s)	412
Esp3I	CGTCTC	3 cut(s)	644, 977, 979
FaqI	GGGAC	2 cut(s)	697, 1555
FauNDI	CATATG	1 cut(s)	621
FbaI	TGATCA	1 cut(s)	616
FokI	GGATG	3 cut(s)	503, 647, 693
FspBI	CTAG	8 cut(s)	305, 669, 681, 762, 858, 1271, 1572, 1620
FspI	TGCGCA	1 cut(s)	295
GlaI	GCGC	3 cut(s)	295, 566, 578
HaeII	RGCGCY	1 cut(s)	568
HaeIII	GGCC	7 cut(s)	362, 634, 766, 871, 941, 1016, 1631
HapII	CCGG	2 cut(s)	106, 480
HgaI	GACGC	1 cut(s)	996
HhaI	GCGC	3 cut(s)	296, 567, 579
Hin6I	GCGC	3 cut(s)	294, 565, 577
HinP1I	GCGC	3 cut(s)	294, 565, 577
HinfI	GANTC	7 cut(s)	451, 536, 1083, 1114, 1121, 1192, 1314
HpaII	CCGG	2 cut(s)	106, 480
HphI	GGTGA	5 cut(s)	713, 747, 1385, 1460, 1498
Hpy166II	GTNNAC	3 cut(s)	674, 690, 1412
Hpy188I	TCNGA	6 cut(s)	315, 471, 796, 1144, 1319, 1658
Hpy188III	TCNNGA	6 cut(s)	216, 253, 305, 1075, 1087, 1118
Hpy8I	GTNNAC	3 cut(s)	674, 690, 1412
HpyAV	CCTTC	5 cut(s)	109, 279, 580, 1061, 1514
HpyCH4III	ACNGT	3 cut(s)	935, 1034, 1483
HpyF10VI	GCNNNNNNNGC	6 cut(s)	86, 333, 435, 631, 889, 1532
HpyF3I	CTNAG	8 cut(s)	47, 172, 214, 282, 314, 453, 920, 1364
HspAI	GCGC	3 cut(s)	294, 565, 577
Ksp22I	TGATCA	1 cut(s)	616
Kzo9I	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
LguI	GCTCTTC	1 cut(s)	742
LmnI	GCTCC	5 cut(s)	14, 26, 374, 572, 1523
LweI	GCATC	5 cut(s)	38, 402, 1144, 1299, 1540
MaeI	CTAG	8 cut(s)	305, 669, 681, 762, 858, 1271, 1572, 1620
MaeIII	GTNAC	6 cut(s)	257, 317, 421, 747, 946, 1266
MalI	GATC	9 cut(s)	126, 141, 251, 618, 1059, 1073, 1101, 1346, 1608
MbiI	CCGCTC	1 cut(s)	1526
MboI	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
MfeI	CAATTG	1 cut(s)	606
MflI	RGATCY	2 cut(s)	124, 1057
MhlI	GDGCHC	4 cut(s)	371, 445, 676, 692
MlsI	TGGCCA	1 cut(s)	941
MluCI	AATT	6 cut(s)	242, 286, 606, 628, 818, 1397
MluNI	TGGCCA	1 cut(s)	941
MlyI	GAGTC	3 cut(s)	445, 530, 1130
MmeI	TCCRAC	1 cut(s)	667
Mox20I	TGGCCA	1 cut(s)	941
Mph1103I	ATGCAT	1 cut(s)	1254
MscI	TGGCCA	1 cut(s)	941
MslI	CAYNNNNRTG	4 cut(s)	417, 591, 1419, 1641
Msp20I	TGGCCA	1 cut(s)	941
MspA1I	CMGCKG	4 cut(s)	80, 185, 457, 962
MspI	CCGG	2 cut(s)	106, 480
MspR9I	CCNGG	4 cut(s)	106, 342, 480, 481
MunI	CAATTG	1 cut(s)	606
MvaI	CCWGG	1 cut(s)	342
MwoI	GCNNNNNNNGC	6 cut(s)	86, 333, 435, 631, 889, 1532
NciI	CCSGG	3 cut(s)	106, 480, 481
NcoI	CCATGG	1 cut(s)	412
NdeI	CATATG	1 cut(s)	621
NdeII	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
NheI	GCTAGC	1 cut(s)	857
NlaIV	GGNNCC	3 cut(s)	97, 126, 487
NsbI	TGCGCA	1 cut(s)	295
NsiI	ATGCAT	1 cut(s)	1254
PciSI	GCTCTTC	1 cut(s)	742
PcsI	WCGNNNNNNNCGW	1 cut(s)	245
PfeI	GAWTC	4 cut(s)	1083, 1114, 1192, 1314
PflFI	GACNNNGTC	1 cut(s)	845
PfoI	TCCNGGA	1 cut(s)	104
PleI	GAGTC	3 cut(s)	445, 530, 1129
PpsI	GAGTC	3 cut(s)	445, 530, 1129
Psp6I	CCWGG	1 cut(s)	340
PspGI	CCWGG	1 cut(s)	340
PspN4I	GGNNCC	3 cut(s)	97, 126, 487
PspPI	GGNCC	4 cut(s)	361, 869, 1015, 1629
PstNI	CAGNNNCTG	3 cut(s)	161, 965, 1656
PsuI	RGATCY	2 cut(s)	124, 1057
PsyI	GACNNNGTC	1 cut(s)	845
PvuII	CAGCTG	3 cut(s)	80, 457, 962
RseI	CAYNNNNRTG	4 cut(s)	417, 591, 1419, 1641
SapI	GCTCTTC	1 cut(s)	742
Sau3AI	GATC	9 cut(s)	124, 139, 249, 616, 1057, 1071, 1099, 1344, 1606
Sau96I	GGNCC	4 cut(s)	361, 869, 1015, 1629
SchI	GAGTC	3 cut(s)	445, 530, 1130
ScrFI	CCNGG	4 cut(s)	106, 342, 480, 481
SduI	GDGCHC	4 cut(s)	371, 445, 676, 692
SfaNI	GCATC	5 cut(s)	38, 402, 1144, 1299, 1540
SfcI	CTRYAG	2 cut(s)	730, 1648
SmaI	CCCGGG	1 cut(s)	481
SmiMI	CAYNNNNRTG	4 cut(s)	417, 591, 1419, 1641
SmlI	CTYRAG	2 cut(s)	877, 887
SmoI	CTYRAG	2 cut(s)	877, 887
Sse9I	AATT	6 cut(s)	242, 286, 606, 628, 818, 1397
SsiI	CCGC	3 cut(s)	185, 409, 1526
SspMI	CTAG	8 cut(s)	305, 669, 681, 762, 858, 1271, 1572, 1620
StyD4I	CCNGG	4 cut(s)	104, 340, 478, 479
StyI	CCWWGG	1 cut(s)	412
TaaI	ACNGT	3 cut(s)	935, 1034, 1483
TaqI	TCGA	1 cut(s)	138
TasI	AATT	6 cut(s)	242, 286, 606, 628, 818, 1397
TauI	GCSGC	1 cut(s)	1529
TfiI	GAWTC	4 cut(s)	1083, 1114, 1192, 1314
TscAI	CASTG	5 cut(s)	166, 371, 899, 909, 1582
TspDTI	ATGAA	2 cut(s)	729, 1199
TspGWI	ACGGA	4 cut(s)	254, 542, 699, 802
TspMI	CCCGGG	1 cut(s)	479
TspRI	CASTG	5 cut(s)	166, 371, 899, 909, 1582
Tth111I	GACNNNGTC	1 cut(s)	845
VneI	GTGCAC	2 cut(s)	672, 688
XapI	RAATTY	2 cut(s)	242, 286
XbaI	TCTAGA	1 cut(s)	304
XmaI	CCCGGG	1 cut(s)	479
XspI	CTAG	8 cut(s)	305, 669, 681, 762, 858, 1271, 1572, 1620
Zsp2I	ATGCAT	1 cut(s)	1254

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.