Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009252

Physical Location & Seq

Genomic Coordinates Reverse (-)

3011874 .. 3017375

Gene Structure 5502 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1024700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCCGCGCTGCAGTACCTGGAGTCGCTGCGCAACGCGCATCCGGAGCTCGCCGAATGGTACAATTCACTCGCAGATCTGTACCAAAGGAAGCTATGGCACCAGCTCACTCTCAAGCTCGAGCAGTTCGTTGCTCTCGCCGTCTTTCAGGCTGGTGATGCTCTGATACAGTTATACCACAATTTCATCACCGATTTTGAGACTAAAATCAACCTTCTCAAGCTTGCACACTTTGCAGTCATTGTTTCTCGACAGTACCCAGAGAAGGAAGCTGCTATCAGTTACCTAGAAGGGGTGATTGAGAAGCTTCAAGCCACTAGAGAGCAACGCATAGAGGAACCAATCCTTTACATTAAGATGCAAATAGCAATATTCAAACTTGAACAACAAGACCAAAAGGGGTGCAAAAATCTTCTAGAAGATGGAAAGACTACACTTGACAGTATGACTGATATTGATCCGTCTGTGTATGCTAGCTATTATTGGGTGTCATCTCAATATCATAAGTTCCGTCAAGAATTTGCTGAGTTCTACAAAAGTGCTCTTCTTTATCTGGCATACACATCAGTGGAGTCTCTCTCAGAATCATTCAAGCTGGATCTGGCATTTGATTTGTCCCTTTCTGCGCTCCTGGGAGATAACATCTACAACTTTGGAGAGCTGCTTGCGCATCCTATTATTAAGTCTCTTCTTGGGACAAAGGTAGAGTGGCTTTACTATATTCTTCAAGCGTTCAACTCTGGTGATTTAGTTCGGTATCAAGAGTTATGCCGTGTGCACAATGCTGCTCTGAGAGCACAACCGGCATTAGTTGATAATGAGAAGAAGCTTTTGGAGAAGATTAACATTCTGTGCTTGATGGAAATTATCTTCAGCCGGCCAGCTGAAGATCGGACTATACCATTGAGTATCATTGCAGAGCGTACCAAACTTTCAGTCGAGGATGTGGAGCATCTTCTTGTGAAGAGCCTTTCCGTTCATCTGATCGAGGGAATAATCGATCAGGTCGAGGAGACAGTGCATGTGTCCTGGGTGCAGCCAAGAGTTTTGGGGATTCCACAGGTCAAGTCATTGCGTGATAGGTTGGACAGTTGGTTGGACAAAGTCCATACTGCTTTGTTATCTATCGAGGCGGAAACACCTGATTTGGTTGCATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008541 GO:0009056 GO:0009057 GO:0009987 GO:0016043 GO:0019538 GO:0019941 GO:0022607 GO:0022624 GO:0030163 GO:0032991 GO:0034622 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0065003 GO:0071704 GO:0071840 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

PCI

Protein Analysis

387

Amino Acids

44.38

Weight (kDa)

5.25

Isoelectric Point (pI)

35.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PSD13_N	PF22037	21 - 242	1e-73	PSD13 N-terminal repeats
PCI	PF01399	263 - 344	3.4e-09	PCI domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009252)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19006 AT4G19006 AT5G45620 AT5G45620
fragaria_vesca	FvH4_5g23350 FvH4_5g23350 FvH4_5g23350
malus_domestica	MD06G1024700.v1.1 MD16G1281300.v1.1
prunus_persica	Prupe.5G025900_v2.0.a1
pyrus_communis	pycom06g02000 pycom16g25120
rosa_chinensis	RchiOBHm_Chr7g0212291
rosa_laevigata	RLG00000002897
rosa_multiflora	Rmu_sc0001911.1_g000049
rosa_roxburghii	Rroxscaffold_3G00246920
rosa_rugosa	Rorug07G0133400
rosa_samantha	Rh7AG266000 Rh7BG260100 Rh7CG282900 Rh7DG272900
rosa_wichuraiana	Rw7G022770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	32, 669
AccB1I	GGYRCC	1 cut(s)	99
AccII	CGCG	2 cut(s)	8, 38
AccIII	TCCGGA	1 cut(s)	43
AciI	CCGC	2 cut(s)	6, 1133
AclWI	GGATC	2 cut(s)	452, 606
AcoI	YGGCCR	2 cut(s)	3, 878
AcsI	RAATTY	1 cut(s)	518
AcuI	CTGAAG	2 cut(s)	856, 906
AfaI	GTAC	5 cut(s)	17, 62, 83, 257, 925
AfiI	CCNNNNNNNGG	2 cut(s)	265, 292
AgsI	TTSAA	6 cut(s)	311, 376, 383, 592, 728, 736
AjnI	CCWGG	3 cut(s)	18, 630, 1028
AjuI	GAANNNNNNNTTGG	2 cut(s)	815, 847
AleI	CACNNNNGTG	1 cut(s)	566
Alw21I	GWGCWC	4 cut(s)	51, 544, 780, 799
Alw26I	GTCTC	4 cut(s)	194, 579, 690, 1007
Alw44I	GTGCAC	1 cut(s)	776
AlwI	GGATC	2 cut(s)	452, 606
AlwNI	CAGNNNCTG	1 cut(s)	19
Ama87I	CYCGRG	1 cut(s)	119
Aor13HI	TCCGGA	1 cut(s)	43
AoxI	GGCC	2 cut(s)	3, 878
ApaLI	GTGCAC	1 cut(s)	776
ApeKI	GCWGC	6 cut(s)	10, 28, 272, 661, 785, 1036
ApoI	RAATTY	1 cut(s)	518
ArsI	GACNNNNNNTTYG	2 cut(s)	921, 953
AspLEI	GCGC	5 cut(s)	10, 33, 40, 628, 670
AsuHPI	GGTGA	4 cut(s)	167, 181, 307, 755
AsuNHI	GCTAGC	1 cut(s)	473
AvaI	CYCGRG	1 cut(s)	119
BaeGI	GKGCMC	1 cut(s)	780
BanI	GGYRCC	1 cut(s)	99
BanII	GRGCYC	1 cut(s)	51
Bbv12I	GWGCWC	4 cut(s)	51, 544, 780, 799
BbvI	GCAGC	5 cut(s)	15, 259, 648, 772, 1048
BccI	CCATC	2 cut(s)	416, 853
BceAI	ACGGC	2 cut(s)	125, 756
BciT130I	CCWGG	3 cut(s)	20, 632, 1030
BcoDI	GTCTC	4 cut(s)	194, 579, 690, 1007
BfaI	CTAG	4 cut(s)	287, 318, 416, 474
BfmI	CTRYAG	1 cut(s)	11
BglII	AGATCT	1 cut(s)	76
BisI	GCNGC	7 cut(s)	6, 11, 29, 273, 662, 786, 1037
BlsI	GCNGC	7 cut(s)	7, 12, 30, 274, 663, 787, 1038
Bme1390I	CCNGG	3 cut(s)	20, 632, 1030
BmeT110I	CYCGRG	1 cut(s)	119
BmiI	GGNNCC	2 cut(s)	101, 339
BmrFI	CCNGG	3 cut(s)	20, 632, 1030
BmsI	GCATC	5 cut(s)	49, 148, 348, 679, 961
BmtI	GCTAGC	1 cut(s)	477
BplI	GAGNNNNNCTC	2 cut(s)	563, 595
BpmI	CTGGAG	1 cut(s)	41
BpuEI	CTTGAG	2 cut(s)	98, 203
Bsa29I	ATCGAT	1 cut(s)	999
BsaBI	GATNNNNATC	1 cut(s)	456
BsaJI	CCNNGG	2 cut(s)	631, 1029
BsaWI	WCCGGW	1 cut(s)	43
Bsc4I	CCNNNNNNNGG	2 cut(s)	265, 292
Bse118I	RCCGGY	2 cut(s)	802, 876
Bse3DI	GCAATG	2 cut(s)	912, 1070
Bse8I	GATNNNNATC	1 cut(s)	456
BseAI	TCCGGA	1 cut(s)	43
BseBI	CCWGG	3 cut(s)	20, 632, 1030
BseCI	ATCGAT	1 cut(s)	999
BseDI	CCNNGG	2 cut(s)	631, 1029
BseGI	GGATG	3 cut(s)	40, 670, 949
BseJI	GATNNNNATC	1 cut(s)	456
BseLI	CCNNNNNNNGG	2 cut(s)	265, 292
BseMI	GCAATG	2 cut(s)	912, 1070
BseMII	CTCAG	3 cut(s)	516, 594, 782
BseRI	GAGGAG	1 cut(s)	1025
BseSI	GKGCMC	1 cut(s)	780
BseXI	GCAGC	5 cut(s)	15, 259, 648, 772, 1048
BsgI	GTGCAG	1 cut(s)	1055
Bsh1236I	CGCG	2 cut(s)	8, 38
BshFI	GGCC	2 cut(s)	5, 880
BshNI	GGYRCC	1 cut(s)	99
BshVI	ATCGAT	1 cut(s)	999
BsiHKAI	GWGCWC	4 cut(s)	51, 544, 780, 799
BsiHKCI	CYCGRG	1 cut(s)	119
BsiSI	CCGG	3 cut(s)	44, 803, 877
BslFI	GGGAC	2 cut(s)	601, 709
BslI	CCNNNNNNNGG	2 cut(s)	265, 292
BsmAI	GTCTC	4 cut(s)	194, 579, 690, 1007
BsmFI	GGGAC	2 cut(s)	601, 709
BsnI	GGCC	2 cut(s)	5, 880
BsoBI	CYCGRG	1 cut(s)	119
Bsp1286I	GDGCHC	4 cut(s)	51, 544, 780, 799
Bsp13I	TCCGGA	1 cut(s)	43
Bsp143I	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
BspACI	CCGC	2 cut(s)	6, 1133
BspANI	GGCC	2 cut(s)	5, 880
BspCNI	CTCAG	3 cut(s)	517, 593, 783
BspDI	ATCGAT	1 cut(s)	999
BspEI	TCCGGA	1 cut(s)	43
BspFNI	CGCG	2 cut(s)	8, 38
BspHI	TCATGA	1 cut(s)	1157
BspLI	GGNNCC	2 cut(s)	101, 339
BspMAI	CTGCAG	1 cut(s)	15
BspOI	GCTAGC	1 cut(s)	477
BspPI	GGATC	2 cut(s)	452, 606
BspQI	GCTCTTC	2 cut(s)	549, 959
BspT107I	GGYRCC	1 cut(s)	99
BsrDI	GCAATG	2 cut(s)	912, 1070
BsrFI	RCCGGY	2 cut(s)	802, 876
BssAI	RCCGGY	2 cut(s)	802, 876
BssECI	CCNNGG	2 cut(s)	631, 1029
BssMI	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
Bst2UI	CCWGG	3 cut(s)	20, 632, 1030
Bst4CI	ACNGT	5 cut(s)	171, 255, 443, 1018, 1091
Bst6I	CTCTTC	3 cut(s)	549, 693, 959
BstAPI	GCANNNNNTGC	1 cut(s)	233
BstC8I	GCNNGC	6 cut(s)	51, 225, 475, 666, 878, 882
BstDEI	CTNAG	3 cut(s)	525, 580, 791
BstF5I	GGATG	3 cut(s)	40, 670, 949
BstFNI	CGCG	2 cut(s)	8, 38
BstHHI	GCGC	5 cut(s)	10, 33, 40, 628, 670
BstKTI	GATC	6 cut(s)	79, 460, 601, 892, 987, 1003
BstMAI	GTCTC	4 cut(s)	194, 579, 690, 1007
BstMBI	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
BstMWI	GCNNNNNNNGC	7 cut(s)	37, 46, 158, 233, 667, 794, 803
BstNI	CCWGG	3 cut(s)	20, 632, 1030
BstNSI	RCATGY	1 cut(s)	1025
BstSCI	CCNGG	3 cut(s)	18, 630, 1028
BstSFI	CTRYAG	1 cut(s)	11
BstSLI	GKGCMC	1 cut(s)	780
BstUI	CGCG	2 cut(s)	8, 38
BstV1I	GCAGC	5 cut(s)	15, 259, 648, 772, 1048
BstX2I	RGATCY	2 cut(s)	76, 598
BstYI	RGATCY	2 cut(s)	76, 598
Bsu15I	ATCGAT	1 cut(s)	999
BsuRI	GGCC	2 cut(s)	5, 880
BsuTUI	ATCGAT	1 cut(s)	999
BtsCI	GGATG	3 cut(s)	40, 670, 949
BtsIMutI	CAGTG	2 cut(s)	573, 1023
Cac8I	GCNNGC	6 cut(s)	51, 225, 475, 666, 878, 882
CaiI	CAGNNNCTG	1 cut(s)	19
CciI	TCATGA	1 cut(s)	1157
CfoI	GCGC	5 cut(s)	10, 33, 40, 628, 670
Cfr10I	RCCGGY	2 cut(s)	802, 876
ClaI	ATCGAT	1 cut(s)	999
Csp6I	GTAC	5 cut(s)	16, 61, 82, 256, 924
CviAII	CATG	2 cut(s)	1022, 1158
CviQI	GTAC	5 cut(s)	16, 61, 82, 256, 924
DdeI	CTNAG	3 cut(s)	525, 580, 791
DpnI	GATC	6 cut(s)	78, 459, 600, 891, 986, 1002
DpnII	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
EaeI	YGGCCR	2 cut(s)	3, 878
Eam1104I	CTCTTC	3 cut(s)	549, 693, 959
EarI	CTCTTC	3 cut(s)	549, 693, 959
EciI	GGCGGA	1 cut(s)	1148
Ecl136II	GAGCTC	1 cut(s)	49
Eco24I	GRGCYC	1 cut(s)	51
Eco53kI	GAGCTC	1 cut(s)	49
Eco57I	CTGAAG	2 cut(s)	856, 906
Eco88I	CYCGRG	1 cut(s)	119
EcoICRI	GAGCTC	1 cut(s)	49
EcoRII	CCWGG	3 cut(s)	18, 630, 1028
EcoT38I	GRGCYC	1 cut(s)	51
FaeI	CATG	2 cut(s)	1025, 1161
FaqI	GGGAC	2 cut(s)	601, 709
FatI	CATG	2 cut(s)	1021, 1157
Fnu4HI	GCNGC	7 cut(s)	6, 11, 29, 273, 662, 786, 1037
FokI	GGATG	3 cut(s)	27, 657, 956
FriOI	GRGCYC	1 cut(s)	51
Fsp4HI	GCNGC	7 cut(s)	6, 11, 29, 273, 662, 786, 1037
FspBI	CTAG	4 cut(s)	287, 318, 416, 474
FspI	TGCGCA	2 cut(s)	32, 669
GlaI	GCGC	5 cut(s)	9, 32, 39, 627, 669
GluI	GCNGC	7 cut(s)	6, 11, 29, 273, 662, 786, 1037
GsuI	CTGGAG	1 cut(s)	41
HaeIII	GGCC	2 cut(s)	5, 880
HapII	CCGG	3 cut(s)	44, 803, 877
HhaI	GCGC	5 cut(s)	10, 33, 40, 628, 670
Hin1II	CATG	2 cut(s)	1025, 1161
Hin6I	GCGC	5 cut(s)	8, 31, 38, 626, 668
HinP1I	GCGC	5 cut(s)	8, 31, 38, 626, 668
HindIII	AAGCTT	3 cut(s)	221, 305, 827
HinfI	GANTC	4 cut(s)	23, 572, 584, 1054
HpaII	CCGG	3 cut(s)	44, 803, 877
HphI	GGTGA	4 cut(s)	167, 181, 307, 755
Hpy166II	GTNNAC	1 cut(s)	778
Hpy188I	TCNGA	5 cut(s)	165, 583, 792, 894, 984
Hpy188III	TCNNGA	6 cut(s)	44, 249, 416, 515, 761, 1158
Hpy8I	GTNNAC	1 cut(s)	778
HpyAV	CCTTC	3 cut(s)	224, 259, 284
HpyCH4III	ACNGT	5 cut(s)	171, 255, 443, 1018, 1091
HpyF10VI	GCNNNNNNNGC	7 cut(s)	37, 46, 158, 233, 667, 794, 803
HpyF3I	CTNAG	3 cut(s)	525, 580, 791
Hsp92II	CATG	2 cut(s)	1025, 1161
HspAI	GCGC	5 cut(s)	8, 31, 38, 626, 668
Kpn2I	TCCGGA	1 cut(s)	43
KroI	GCCGGC	1 cut(s)	876
KroNI	GCCGGC	1 cut(s)	878
Kzo9I	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
LguI	GCTCTTC	2 cut(s)	549, 959
LmnI	GCTCC	3 cut(s)	46, 633, 949
Lsp1109I	GCAGC	5 cut(s)	15, 259, 648, 772, 1048
LweI	GCATC	5 cut(s)	49, 148, 348, 679, 961
MaeI	CTAG	4 cut(s)	287, 318, 416, 474
MaeIII	GTNAC	1 cut(s)	281
MalI	GATC	6 cut(s)	78, 459, 600, 891, 986, 1002
MboI	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
MflI	RGATCY	2 cut(s)	76, 598
MhlI	GDGCHC	4 cut(s)	51, 544, 780, 799
MluCI	AATT	4 cut(s)	64, 181, 518, 864
MlyI	GAGTC	2 cut(s)	32, 581
MmeI	TCCRAC	2 cut(s)	1065, 1077
MnlI	CCTC	5 cut(s)	328, 934, 982, 1003, 1123
MroI	TCCGGA	1 cut(s)	43
MroNI	GCCGGC	1 cut(s)	876
MseI	TTAA	3 cut(s)	354, 681, 843
MslI	CAYNNNNRTG	2 cut(s)	356, 566
MspA1I	CMGCKG	1 cut(s)	884
MspI	CCGG	3 cut(s)	44, 803, 877
MspR9I	CCNGG	3 cut(s)	20, 632, 1030
MvaI	CCWGG	3 cut(s)	20, 632, 1030
MvnI	CGCG	2 cut(s)	8, 38
MwoI	GCNNNNNNNGC	7 cut(s)	37, 46, 158, 233, 667, 794, 803
NaeI	GCCGGC	1 cut(s)	878
NdeII	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
NgoMIV	GCCGGC	1 cut(s)	876
NheI	GCTAGC	1 cut(s)	473
NlaIII	CATG	2 cut(s)	1025, 1161
NlaIV	GGNNCC	2 cut(s)	101, 339
NsbI	TGCGCA	2 cut(s)	32, 669
NspI	RCATGY	1 cut(s)	1025
OliI	CACNNNNGTG	1 cut(s)	566
PaeR7I	CTCGAG	1 cut(s)	119
PagI	TCATGA	1 cut(s)	1157
PciSI	GCTCTTC	2 cut(s)	549, 959
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 1005
PdiI	GCCGGC	1 cut(s)	878
PfeI	GAWTC	2 cut(s)	584, 1054
PflFI	GACNNNGTC	1 cut(s)	1103
PkrI	GCNGC	7 cut(s)	7, 12, 30, 274, 663, 787, 1038
PleI	GAGTC	2 cut(s)	31, 580
PpsI	GAGTC	2 cut(s)	31, 580
Psp124BI	GAGCTC	1 cut(s)	51
Psp6I	CCWGG	3 cut(s)	18, 630, 1028
PspGI	CCWGG	3 cut(s)	18, 630, 1028
PspN4I	GGNNCC	2 cut(s)	101, 339
PspXI	VCTCGAGB	1 cut(s)	119
PstI	CTGCAG	1 cut(s)	15
PstNI	CAGNNNCTG	1 cut(s)	19
PsuI	RGATCY	2 cut(s)	76, 598
PsyI	GACNNNGTC	1 cut(s)	1103
PvuII	CAGCTG	1 cut(s)	884
RsaI	GTAC	5 cut(s)	17, 62, 83, 257, 925
RsaNI	GTAC	5 cut(s)	16, 61, 82, 256, 924
RseI	CAYNNNNRTG	2 cut(s)	356, 566
SacI	GAGCTC	1 cut(s)	51
SapI	GCTCTTC	2 cut(s)	549, 959
SaqAI	TTAA	3 cut(s)	354, 681, 843
SatI	GCNGC	7 cut(s)	6, 11, 29, 273, 662, 786, 1037
Sau3AI	GATC	6 cut(s)	76, 457, 598, 889, 984, 1000
SchI	GAGTC	2 cut(s)	32, 581
ScrFI	CCNGG	3 cut(s)	20, 632, 1030
SduI	GDGCHC	4 cut(s)	51, 544, 780, 799
SfaNI	GCATC	5 cut(s)	49, 148, 348, 679, 961
SfcI	CTRYAG	1 cut(s)	11
Sfr274I	CTCGAG	1 cut(s)	119
SlaI	CTCGAG	1 cut(s)	119
SmiMI	CAYNNNNRTG	2 cut(s)	356, 566
SmlI	CTYRAG	3 cut(s)	113, 119, 218
SmoI	CTYRAG	3 cut(s)	113, 119, 218
Sse9I	AATT	4 cut(s)	64, 181, 518, 864
SsiI	CCGC	2 cut(s)	6, 1133
SspI	AATATT	1 cut(s)	372
SspMI	CTAG	4 cut(s)	287, 318, 416, 474
SstI	GAGCTC	1 cut(s)	51
StyD4I	CCNGG	3 cut(s)	18, 630, 1028
TaaI	ACNGT	5 cut(s)	171, 255, 443, 1018, 1091
TaqI	TCGA	7 cut(s)	120, 250, 939, 987, 999, 1008, 1128
TasI	AATT	4 cut(s)	64, 181, 518, 864
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	2 cut(s)	584, 1054
Tru1I	TTAA	3 cut(s)	354, 681, 843
Tru9I	TTAA	3 cut(s)	354, 681, 843
TscAI	CASTG	2 cut(s)	573, 1023
TseI	GCWGC	6 cut(s)	10, 28, 272, 661, 785, 1036
TspDTI	ATGAA	2 cut(s)	175, 968
TspGWI	ACGGA	3 cut(s)	450, 500, 964
TspRI	CASTG	2 cut(s)	573, 1023
Tth111I	GACNNNGTC	1 cut(s)	1103
VneI	GTGCAC	1 cut(s)	776
XapI	RAATTY	1 cut(s)	518
XbaI	TCTAGA	1 cut(s)	415
XceI	RCATGY	1 cut(s)	1025
XhoI	CTCGAG	1 cut(s)	119
XspI	CTAG	4 cut(s)	287, 318, 416, 474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.