MD06G1026100.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007240
Physical Location & Seq
Forward (+)
3210489 .. 3217400
6912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1026100.v1.1.491

Sequence Viewer

Length: 2673 bp
ATGGTGGAAGGAAGGGTTTGTTTGTCCAAAGAGGCCAAAAATGGATTAGAAATTCTGAAGCGTAGAAGGCTTCAGCGAATGAGACCAGAAACTGCTACCGAGCCTGTAAATCATACTAATACGATGGCTCGAAGTGGAGGTGATGCTTTAAAACCTTCTGTATCCTTTGGTGTTAGATTACAGGGAGGTGCCGACTCAGTTTCTCTGTCCAGTGGTGCTATGCATGAAAAAGATGTTTTTTCGAAGCGTAGGGTGGATAAGTTTGAAACAAGTGATCTAGAGTGGATTGAGAAAATTCCCGAGTGTCCTGTATACTATCCAGCAATGGAGGAGTTTGATGATCCTTTGGTTTATTTACAGAAGATAGCTCCAGAAGCTTCAAAATATGGTATATGCAAGATTGTCTCTCCTTTGAGTGCTTCTACTCCAGCTGGGGTTGTATTGATGAAAGAGAAAGCAGGATTCAAGTTCACAACTAGAGTACAACCTCTTCGTCTTGCAGAGTGGGACAATGATGACAAGGTCACCTTTTTCATGAGCGGAAGAAATTATACATTCCGTGATTTCGAGAAAATGGCGAACAAGGTTTTTGCTCGTAGATATTGTAGTACCGGTTCTCTTCCTGCCTCGTTCTTGGAAAAAGAATTTTGGCAAGAAATTTCTTGTGGAAAGACAGAAACTGTTGAGTATGCTTGTGATGTAGATGGTAGTGCCTTTTCATCTTCTCACAGTGATCCTCTTGGAAATAGCAAATGGAATTTAAAGAATCTTTCAAGGTTGCCCAAGTCCATTTTGCATCTTCTAGAAACTGCAATTCCGGGTGTAACGGATCCCATGCTATACATTGGAATGCTATTTAGTATGTTTGCTTGGCACGTGGAGGATCATTATTTGTACAGTATTAATTATCATCATTGTGGCGCATCCAAAACCTGGTATGGCATTCCAGGTGAAGCTGCTCTGCAATTTGAAAAGGTTGTTAAGGAACATGTGTACACGCATGATATCATATCAACTGATGGAGAAGATGGAGCATTTGATGTCTTGTTGGGGAAAACAACATTGTTTCCTCCAAATATTTTGTTGGAACATGATGTCCCTGTATACAAGGCTGTGCAAAAGCCAGGAGAGTTCGTTATTACTTTCCCTAGAGCATACCATGCTGGATTCAGTCATGGTTTCAACTGTGGCGAGGCAGTCAACTTTGCAATTGGTGATTGGTTCCCTTTAGGTGCTATTGCTAGCCGGCGTTATGCGCTGCTTAACAGGATGCCTCTGCTTCCTCATGAAGAACTTCTGTGTAAAGAAGCGATGCTTCTACACACAAGTTTAGAACTTGAAGATTCGTATTATTCATCTGCTGATTTGGTGTCCCATGATAGCATTAAGATGTCTTTTGTGAGATTGATGCGCTTTCAGCATCGTGCTCGTTGGTCTTTAATGAAGTCAGGGGCATGCACCAGTGTTTTGCCAAACTCCTATGGAACCATTCTATGCAGCCTATGCAAGCGTGATTGCTACATAGCTTTCATTAATTGCAATTGTTACATGCATCCTGTCTGCCTTCGCCATGATGTAAGGTCTCTTGACTTCTCATGTGGGACCAATCCTACTCTCTTTTTAAGGGAGGAAATAACAGAATTGGAAGCTGCAGCCAGAAAATTTGAAGAGGACGATGGTGTGTTGGAGGCGATAAAAGGGCTAGGCGATGACTTGTATTCATATCCATTAAATTTGTCTCAGAGCGCTGAAGAGAAAGGATACTCTCGTTATTGTGAGATAAAATTTGAGTTGGACACTGAACTAGCTGGCACAACTCAGTATCAGTCACAAGAGGCAGAAACAGCCCAAACGGGTTCTCATGGCCAGCCCATGTTGAGATCTGATGCAAAATATTCAAGTCCTGCAGTGTCAGACGGTTCCCTCTCATGTGCTGCATCAACTCTTTGTTCTCTTCTAGAGCCTCGTGAGAGCTCATCTGCACCCAATAATGTACAGGAAAATGCTAAAGAAACTGTAAATTCTAAAAGGCATTCTGAAGAAGTGGTTCGCAGTGTTTACGATTCTTCTCTGTCCTCTCCATCATATGATGAATGCTCTAGTGCACGCCCAGGGAATTCTAATGGATCGGAGGTTAGACGTGTTGTGGATCAAGGCAGTGATGATTCTGATTCAGAGATCTTTAGGGTTAAGCGTCGTTCATCATTGAAGGTGGTGGGCAAAAGGAGCGTAAATGATGTCTCGGCATCAAATCATTCTGAAAACAAGGGGTTTAAACGATTGAAGAAAATGCAACCTGAAAGAAGATGCGGGAGATCATCGGTGCCATTAGATTATTACAGCCCTGGTGAATCAAATACTAAGTTTCTTTCAACCACTAATTACAGAGGATTTCCAGAGAGTGCTGCTTCAGAGGGCAGGTTCTCGACAGGAAGTAGCGCTCCTAGAGGAGGTAATGTTCCCATCTCTATCAAGTTTAAGAAGTTGGCCACCGAAGATTCAGCTAGGAAACAGCGAGAACACCACAGAAAGGATAGATACCAGGATGAATTGGGAAGGAGCCGGAGGCAACCACCCCCACCCCCACCCCCACCCCCAATGGAGGTTGGGGCAAAGCGCATTAAAGTCAAAGGCCCGTCGTTCCTAGGGTCGGAGAGCAGGTTAAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0000792 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010016 GO:0010033 GO:0010228 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032454 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045815 GO:0048366 GO:0048367 GO:0048439 GO:0048518 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

891

Amino Acids

99.49

Weight (kDa)

7.09

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 103 - 135 3.8e-15 jmjN domain
JmjC PF02373 279 - 402 7.9e-40 JmjC domain, hydroxylase
zf-C5HC2 PF02928 499 - 550 1.4e-09 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2409, 2649
AccB1I GGYRCC 2 cut(s) 188, 2323
AccB7I CCANNNNNTGG 1 cut(s) 933
AccBSI CCGCTC 1 cut(s) 540
AccI GTMKAC 2 cut(s) 312, 1104
AciI CCGC 2 cut(s) 540, 2310
AclWI GGATC 7 cut(s) 335, 728, 824, 837, 891, 2134, 2157
AcoI YGGCCR 2 cut(s) 1864, 2487
AcuI CTGAAG 5 cut(s) 56, 77, 1770, 2058, 2394
AcvI CACGTG 1 cut(s) 877
AfaI GTAC 5 cut(s) 483, 610, 896, 995, 1995
AfeI AGCGCT 2 cut(s) 1747, 2440
AfiI CCNNNNNNNGG 5 cut(s) 933, 2450, 2530, 2602, 2650
AflIII ACRYGT 2 cut(s) 988, 2140
AgeI ACCGGT 1 cut(s) 611
AjiI CACGTC 1 cut(s) 2141
AjnI CCWGG 6 cut(s) 932, 946, 1123, 2110, 2344, 2541
AloI GAACNNNNNNTCC 4 cut(s) 1080, 1112, 2442, 2474
AluBI AGCT 9 cut(s) 368, 377, 431, 956, 1526, 1649, 1808, 1974, 2504
AluI AGCT 9 cut(s) 368, 377, 431, 956, 1526, 1649, 1808, 1974, 2504
Alw21I GWGCWC 3 cut(s) 1429, 1976, 2107
Alw26I GTCTC 5 cut(s) 76, 409, 1587, 1743, 2245
Alw44I GTGCAC 1 cut(s) 2103
AlwI GGATC 7 cut(s) 335, 728, 824, 837, 891, 2134, 2157
AlwNI CAGNNNCTG 2 cut(s) 92, 680
Ama87I CYCGRG 1 cut(s) 299
Aor51HI AGCGCT 2 cut(s) 1747, 2440
AoxI GGCC 4 cut(s) 33, 1864, 2487, 2632
ApaLI GTGCAC 1 cut(s) 2103
ApeKI GCWGC 7 cut(s) 956, 1258, 1497, 1649, 1652, 1934, 2405
AseI ATTAAT 2 cut(s) 903, 1533
AsiGI ACCGGT 1 cut(s) 611
Asp700I GAANNNNTTC 2 cut(s) 1293, 2046
AspA2I CCTAGG 1 cut(s) 2644
AspLEI GCGC 6 cut(s) 923, 1258, 1413, 1748, 2441, 2619
AspS9I GGNCC 2 cut(s) 1602, 2633
AsuC2I CCSGG 1 cut(s) 819
AsuHPI GGTGA 5 cut(s) 152, 517, 962, 1226, 2360
AsuII TTCGAA 1 cut(s) 242
AsuNHI GCTAGC 1 cut(s) 1241
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 1602
AvrII CCTAGG 1 cut(s) 2644
BaeGI GKGCMC 1 cut(s) 2107
BaeI ACNNNNGTAYC 2 cut(s) 1753, 1786
BalI TGGCCA 2 cut(s) 1866, 2489
BamHI GGATCC 1 cut(s) 829
BanI GGYRCC 2 cut(s) 188, 2323
BanII GRGCYC 1 cut(s) 1976
BarI GAAGNNNNNNTAC 4 cut(s) 474, 506, 535, 567
BauI CACGAG 1 cut(s) 1965
BbrPI CACGTG 1 cut(s) 877
Bbv12I GWGCWC 3 cut(s) 1429, 1976, 2107
BbvI GCAGC 7 cut(s) 943, 1245, 1509, 1636, 1664, 1921, 2392
BccI CCATC 7 cut(s) 118, 698, 1013, 1022, 1670, 2089, 2471
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 6 cut(s) 934, 948, 1125, 2112, 2346, 2543
BciVI GTATCC 2 cut(s) 172, 1754
BcnI CCSGG 1 cut(s) 819
BcoDI GTCTC 5 cut(s) 76, 409, 1587, 1743, 2245
BfmI CTRYAG 2 cut(s) 1650, 1905
BfoI RGCGCY 2 cut(s) 1749, 2442
BfuAI ACCTGC 2 cut(s) 2409, 2649
BfuI GTATCC 2 cut(s) 172, 1754
BglII AGATCT 2 cut(s) 1880, 2178
BisI GCNGC 7 cut(s) 957, 1259, 1498, 1650, 1653, 1935, 2406
BlnI CCTAGG 1 cut(s) 2644
BlsI GCNGC 7 cut(s) 958, 1260, 1499, 1651, 1654, 1936, 2407
Bme1390I CCNGG 7 cut(s) 819, 934, 948, 1125, 2112, 2346, 2543
Bme18I GGWCC 1 cut(s) 1602
BmeT110I CYCGRG 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 2141
BmgT120I GGNCC 2 cut(s) 1602, 2633
BmiI GGNNCC 8 cut(s) 190, 831, 1223, 1486, 1603, 1921, 2325, 2561
BmrFI CCNGG 7 cut(s) 819, 934, 948, 1125, 2112, 2346, 2543
BmtI GCTAGC 1 cut(s) 1245
BpmI CTGGAG 2 cut(s) 354, 411
Bpu14I TTCGAA 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 819
BsaAI YACGTR 1 cut(s) 877
BsaI GGTCTC 2 cut(s) 76, 1587
BsaJI CCNNGG 4 cut(s) 2110, 2111, 2344, 2644
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 5 cut(s) 1119, 1149, 2442, 2472, 2645
Bsc4I CCNNNNNNNGG 5 cut(s) 933, 2450, 2530, 2602, 2650
Bse118I RCCGGY 2 cut(s) 611, 1245
Bse1I ACTGG 2 cut(s) 210, 1461
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 6 cut(s) 934, 948, 1125, 2112, 2346, 2543
BseDI CCNNGG 4 cut(s) 2110, 2111, 2344, 2644
BseGI GGATG 4 cut(s) 923, 1275, 1552, 2551
BseLI CCNNNNNNNGG 5 cut(s) 933, 2450, 2530, 2602, 2650
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 3 cut(s) 210, 1754, 1832
BseNI ACTGG 2 cut(s) 210, 1461
BseRI GAGGAG 2 cut(s) 344, 2463
BseSI GKGCMC 1 cut(s) 2107
BseXI GCAGC 7 cut(s) 943, 1245, 1509, 1636, 1664, 1921, 2392
BseYI CCCAGC 1 cut(s) 431
BsgI GTGCAG 1 cut(s) 1965
BshFI GGCC 4 cut(s) 35, 1866, 2489, 2634
BshNI GGYRCC 2 cut(s) 188, 2323
BshTI ACCGGT 1 cut(s) 611
BsiHKAI GWGCWC 3 cut(s) 1429, 1976, 2107
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 612, 818, 1246, 2563
BslFI GGGAC 4 cut(s) 521, 1082, 1357, 1615
BslI CCNNNNNNNGG 5 cut(s) 933, 2450, 2530, 2602, 2650
BsmAI GTCTC 5 cut(s) 76, 409, 1587, 1743, 2245
BsmFI GGGAC 4 cut(s) 521, 1082, 1357, 1615
BsmI GAATGC 4 cut(s) 855, 942, 2032, 2099
BsnI GGCC 4 cut(s) 35, 1866, 2489, 2634
Bso31I GGTCTC 2 cut(s) 76, 1587
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 1429, 1976, 2107
Bsp1407I TGTACA 3 cut(s) 894, 993, 1993
BspACI CCGC 2 cut(s) 540, 2310
BspANI GGCC 4 cut(s) 35, 1866, 2489, 2634
BspCNI CTCAG 3 cut(s) 209, 1753, 1831
BspHI TCATGA 2 cut(s) 534, 1285
BspLI GGNNCC 8 cut(s) 190, 831, 1223, 1486, 1603, 1921, 2325, 2561
BspMAI CTGCAG 2 cut(s) 1654, 1909
BspMI ACCTGC 2 cut(s) 2409, 2649
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 7 cut(s) 335, 728, 824, 837, 891, 2134, 2157
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 2 cut(s) 188, 2323
BspTNI GGTCTC 2 cut(s) 76, 1587
BsrBI CCGCTC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 2 cut(s) 611, 1245
BsrGI TGTACA 3 cut(s) 894, 993, 1993
BsrI ACTGG 2 cut(s) 210, 1461
BssAI RCCGGY 2 cut(s) 611, 1245
BssECI CCNNGG 4 cut(s) 2110, 2111, 2344, 2644
BssNAI GTATAC 2 cut(s) 313, 1105
BssSI CACGAG 1 cut(s) 1965
BssT1I CCWWGG 1 cut(s) 2644
Bst1107I GTATAC 2 cut(s) 313, 1105
Bst2BI CACGAG 1 cut(s) 1965
Bst2UI CCWGG 6 cut(s) 934, 948, 1125, 2112, 2346, 2543
Bst4CI ACNGT 6 cut(s) 682, 731, 899, 1187, 1919, 2017
Bst6I CTCTTC 5 cut(s) 495, 624, 1662, 1746, 1959
BstAPI GCANNNNNTGC 2 cut(s) 1160, 1503
BstAUI TGTACA 3 cut(s) 894, 993, 1993
BstBAI YACGTR 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 7 cut(s) 1243, 1247, 1456, 1508, 1810, 1868, 2107
BstDEI CTNAG 4 cut(s) 196, 1740, 1818, 2361
BstEII GGTNACC 1 cut(s) 523
BstENI CCTNNNNNAGG 1 cut(s) 2448
BstF5I GGATG 4 cut(s) 923, 1275, 1552, 2551
BstH2I RGCGCY 2 cut(s) 1749, 2442
BstHHI GCGC 6 cut(s) 923, 1258, 1413, 1748, 2441, 2619
BstMAI GTCTC 5 cut(s) 76, 409, 1587, 1743, 2245
BstMWI GCNNNNNNNGC 8 cut(s) 67, 374, 1160, 1255, 1417, 1503, 1844, 2226
BstNI CCWGG 6 cut(s) 934, 948, 1125, 2112, 2346, 2543
BstNSI RCATGY 3 cut(s) 992, 1458, 1552
BstPI GGTNACC 1 cut(s) 523
BstSCI CCNGG 7 cut(s) 817, 932, 946, 1123, 2110, 2344, 2541
BstSFI CTRYAG 2 cut(s) 1650, 1905
BstSLI GKGCMC 1 cut(s) 2107
BstV1I GCAGC 7 cut(s) 943, 1245, 1509, 1636, 1664, 1921, 2392
BstX2I RGATCY 3 cut(s) 829, 1880, 2178
BstYI RGATCY 3 cut(s) 829, 1880, 2178
BstZ17I GTATAC 2 cut(s) 313, 1105
BsuI GTATCC 2 cut(s) 172, 1754
BsuRI GGCC 4 cut(s) 35, 1866, 2489, 2634
BtgZI GCGATG 2 cut(s) 1325, 1722
BtrI CACGTC 1 cut(s) 2141
BtsCI GGATG 4 cut(s) 923, 1275, 1552, 2551
BtsI GCAGTG 3 cut(s) 1914, 2059, 2164
BtsIMutI CAGTG 7 cut(s) 217, 736, 1468, 1797, 1914, 2059, 2164
BveI ACCTGC 2 cut(s) 2409, 2649
Cac8I GCNNGC 7 cut(s) 1243, 1247, 1456, 1508, 1810, 1868, 2107
CaiI CAGNNNCTG 2 cut(s) 92, 680
CciI TCATGA 2 cut(s) 534, 1285
CfoI GCGC 6 cut(s) 923, 1258, 1413, 1748, 2441, 2619
Cfr10I RCCGGY 2 cut(s) 611, 1245
Cfr13I GGNCC 2 cut(s) 1602, 2633
CseI GACGC 1 cut(s) 2183
CsiI ACCWGGT 1 cut(s) 932
Csp6I GTAC 5 cut(s) 482, 609, 895, 994, 1994
CspAI ACCGGT 1 cut(s) 611
CviQI GTAC 5 cut(s) 482, 609, 895, 994, 1994
DdeI CTNAG 4 cut(s) 196, 1740, 1818, 2361
DraI TTTAAA 3 cut(s) 150, 762, 2275
EaeI YGGCCR 2 cut(s) 1864, 2487
Eam1104I CTCTTC 5 cut(s) 495, 624, 1662, 1746, 1959
EarI CTCTTC 5 cut(s) 495, 624, 1662, 1746, 1959
Ecl136II GAGCTC 1 cut(s) 1974
Eco130I CCWWGG 1 cut(s) 2644
Eco24I GRGCYC 1 cut(s) 1976
Eco31I GGTCTC 2 cut(s) 76, 1587
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 1 cut(s) 1602
Eco47III AGCGCT 2 cut(s) 1747, 2440
Eco53kI GAGCTC 1 cut(s) 1974
Eco57I CTGAAG 5 cut(s) 56, 77, 1770, 2058, 2394
Eco72I CACGTG 1 cut(s) 877
Eco88I CYCGRG 1 cut(s) 299
Eco91I GGTNACC 1 cut(s) 523
EcoICRI GAGCTC 1 cut(s) 1974
EcoNI CCTNNNNNAGG 1 cut(s) 2448
EcoO65I GGTNACC 1 cut(s) 523
EcoRI GAATTC 1 cut(s) 2116
EcoRII CCWGG 6 cut(s) 932, 946, 1123, 2110, 2344, 2541
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 2644
EcoT22I ATGCAT 2 cut(s) 225, 1554
EcoT38I GRGCYC 1 cut(s) 1976
ErhI CCWWGG 1 cut(s) 2644
FalI AAGNNNNNCTT 4 cut(s) 512, 544, 1299, 1331
FaqI GGGAC 4 cut(s) 521, 1082, 1357, 1615
FauI CCCGC 1 cut(s) 2303
FauNDI CATATG 1 cut(s) 2086
FblI GTMKAC 2 cut(s) 312, 1104
Fnu4HI GCNGC 7 cut(s) 957, 1259, 1498, 1650, 1653, 1935, 2406
FokI GGATG 4 cut(s) 910, 1282, 1539, 2558
FriOI GRGCYC 1 cut(s) 1976
Fsp4HI GCNGC 7 cut(s) 957, 1259, 1498, 1650, 1653, 1935, 2406
GlaI GCGC 6 cut(s) 922, 1257, 1412, 1747, 2440, 2618
GluI GCNGC 7 cut(s) 957, 1259, 1498, 1650, 1653, 1935, 2406
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 2 cut(s) 354, 411
HaeII RGCGCY 2 cut(s) 1749, 2442
HaeIII GGCC 4 cut(s) 35, 1866, 2489, 2634
HapII CCGG 4 cut(s) 612, 818, 1246, 2563
HgaI GACGC 1 cut(s) 2183
HhaI GCGC 6 cut(s) 923, 1258, 1413, 1748, 2441, 2619
Hin6I GCGC 6 cut(s) 921, 1256, 1411, 1746, 2439, 2617
HinP1I GCGC 6 cut(s) 921, 1256, 1411, 1746, 2439, 2617
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 1 cut(s) 375
HpaII CCGG 4 cut(s) 612, 818, 1246, 2563
HphI GGTGA 5 cut(s) 152, 517, 962, 1226, 2360
Hpy166II GTNNAC 8 cut(s) 313, 471, 994, 996, 1105, 1201, 2059, 2105
Hpy8I GTNNAC 8 cut(s) 313, 471, 994, 996, 1105, 1201, 2059, 2105
Hpy99I CGWCG 2 cut(s) 2199, 2641
HpyAV CCTTC 6 cut(s) 6, 60, 165, 1574, 2203, 2550
HpyCH4III ACNGT 6 cut(s) 682, 731, 899, 1187, 1919, 2017
HpyCH4IV ACGT 2 cut(s) 876, 2140
HpyF10VI GCNNNNNNNGC 8 cut(s) 67, 374, 1160, 1255, 1417, 1503, 1844, 2226
HpyF3I CTNAG 4 cut(s) 196, 1740, 1818, 2361
HpySE526I ACGT 2 cut(s) 876, 2140
HspAI GCGC 6 cut(s) 921, 1256, 1411, 1746, 2439, 2617
KroI GCCGGC 1 cut(s) 1245
KroNI GCCGGC 1 cut(s) 1247
LmnI GCTCC 5 cut(s) 373, 1031, 2226, 2446, 2559
Lsp1109I GCAGC 7 cut(s) 943, 1245, 1509, 1636, 1664, 1921, 2392
MabI ACCWGGT 1 cut(s) 932
MaeII ACGT 2 cut(s) 876, 2140
MaeIII GTNAC 4 cut(s) 523, 823, 1544, 1827
MbiI CCGCTC 1 cut(s) 540
MfeI CAATTG 2 cut(s) 1209, 1540
MflI RGATCY 3 cut(s) 829, 1880, 2178
MhlI GDGCHC 3 cut(s) 1429, 1976, 2107
MlsI TGGCCA 2 cut(s) 1866, 2489
MluNI TGGCCA 2 cut(s) 1866, 2489
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 4 cut(s) 1065, 1665, 1773, 2631
Mox20I TGGCCA 2 cut(s) 1866, 2489
Mph1103I ATGCAT 2 cut(s) 225, 1554
MroNI GCCGGC 1 cut(s) 1245
MroXI GAANNNNTTC 2 cut(s) 1293, 2046
MscI TGGCCA 2 cut(s) 1866, 2489
MslI CAYNNNNRTG 3 cut(s) 848, 915, 1388
Msp20I TGGCCA 2 cut(s) 1866, 2489
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 4 cut(s) 612, 818, 1246, 2563
MspR9I CCNGG 7 cut(s) 819, 934, 948, 1125, 2112, 2346, 2543
MssI GTTTAAAC 1 cut(s) 2275
MunI CAATTG 2 cut(s) 1209, 1540
Mva1269I GAATGC 4 cut(s) 855, 942, 2032, 2099
MvaI CCWGG 6 cut(s) 934, 948, 1125, 2112, 2346, 2543
MwoI GCNNNNNNNGC 8 cut(s) 67, 374, 1160, 1255, 1417, 1503, 1844, 2226
NaeI GCCGGC 1 cut(s) 1247
NciI CCSGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 2086
NgoMIV GCCGGC 1 cut(s) 1245
NheI GCTAGC 1 cut(s) 1241
NlaIV GGNNCC 8 cut(s) 190, 831, 1223, 1486, 1603, 1921, 2325, 2561
NmeAIII GCCGAG 1 cut(s) 2222
NmuCI GTSAC 2 cut(s) 523, 1827
NsiI ATGCAT 2 cut(s) 225, 1554
NspI RCATGY 3 cut(s) 992, 1458, 1552
NspV TTCGAA 1 cut(s) 242
PaeI GCATGC 1 cut(s) 1458
PagI TCATGA 2 cut(s) 534, 1285
PasI CCCWGGG 1 cut(s) 2111
PciI ACATGT 1 cut(s) 988
PctI GAATGC 4 cut(s) 855, 942, 2032, 2099
PdiI GCCGGC 1 cut(s) 1247
PdmI GAANNNNTTC 2 cut(s) 1293, 2046
PfeI GAWTC 9 cut(s) 462, 766, 1167, 1343, 2063, 2165, 2171, 2351, 2498
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 1 cut(s) 933
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 7 cut(s) 958, 1260, 1499, 1651, 1654, 1936, 2407
PleI GAGTC 1 cut(s) 188
PmaCI CACGTG 1 cut(s) 877
PmeI GTTTAAAC 1 cut(s) 2275
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 877
PscI ACATGT 1 cut(s) 988
PshBI ATTAAT 2 cut(s) 903, 1533
Psp124BI GAGCTC 1 cut(s) 1976
Psp6I CCWGG 6 cut(s) 932, 946, 1123, 2110, 2344, 2541
PspCI CACGTG 1 cut(s) 877
PspEI GGTNACC 1 cut(s) 523
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 6 cut(s) 932, 946, 1123, 2110, 2344, 2541
PspN4I GGNNCC 8 cut(s) 190, 831, 1223, 1486, 1603, 1921, 2325, 2561
PspPI GGNCC 2 cut(s) 1602, 2633
PsrI GAACNNNNNNTAC 2 cut(s) 598, 630
PstI CTGCAG 2 cut(s) 1654, 1909
PstNI CAGNNNCTG 2 cut(s) 92, 680
PsuI RGATCY 3 cut(s) 829, 1880, 2178
PsyI GACNNNGTC 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 5 cut(s) 483, 610, 896, 995, 1995
RsaNI GTAC 5 cut(s) 482, 609, 895, 994, 1994
RseI CAYNNNNRTG 3 cut(s) 848, 915, 1388
SacI GAGCTC 1 cut(s) 1976
SatI GCNGC 7 cut(s) 957, 1259, 1498, 1650, 1653, 1935, 2406
Sau96I GGNCC 2 cut(s) 1602, 2633
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 7 cut(s) 819, 934, 948, 1125, 2112, 2346, 2543
SduI GDGCHC 3 cut(s) 1429, 1976, 2107
SexAI ACCWGGT 1 cut(s) 932
SfcI CTRYAG 2 cut(s) 1650, 1905
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 1 cut(s) 1602
SmiMI CAYNNNNRTG 3 cut(s) 848, 915, 1388
SphI GCATGC 1 cut(s) 1458
SsiI CCGC 2 cut(s) 540, 2310
SspI AATATT 2 cut(s) 1078, 1895
SstI GAGCTC 1 cut(s) 1976
StyD4I CCNGG 7 cut(s) 817, 932, 946, 1123, 2110, 2344, 2541
StyI CCWWGG 1 cut(s) 2644
TaaI ACNGT 6 cut(s) 682, 731, 899, 1187, 1919, 2017
TaiI ACGT 2 cut(s) 879, 2143
TaqI TCGA 4 cut(s) 130, 242, 567, 2426
TatI WGTACW 4 cut(s) 481, 894, 993, 1993
TfiI GAWTC 9 cut(s) 462, 766, 1167, 1343, 2063, 2165, 2171, 2351, 2498
TscAI CASTG 7 cut(s) 217, 736, 1468, 1804, 1914, 2059, 2164
TseFI GTSAC 2 cut(s) 523, 1827
TseI GCWGC 7 cut(s) 956, 1258, 1497, 1649, 1652, 1934, 2405
Tsp45I GTSAC 2 cut(s) 523, 1827
TspGWI ACGGA 2 cut(s) 548, 842
TspRI CASTG 7 cut(s) 217, 736, 1468, 1804, 1914, 2059, 2164
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 1 cut(s) 933
VneI GTGCAC 1 cut(s) 2103
VpaK11BI GGWCC 1 cut(s) 1602
VspI ATTAAT 2 cut(s) 903, 1533
XagI CCTNNNNNAGG 1 cut(s) 2448
XbaI TCTAGA 3 cut(s) 277, 802, 1957
XceI RCATGY 3 cut(s) 992, 1458, 1552
XmaJI CCTAGG 1 cut(s) 2644
XmiI GTMKAC 2 cut(s) 312, 1104
XmnI GAANNNNTTC 2 cut(s) 1293, 2046
Zsp2I ATGCAT 2 cut(s) 225, 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.