MD16G1280000.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007240
Physical Location & Seq
Reverse (-)
38164869 .. 38171753
6885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1280000.v1.1.491

Sequence Viewer

Length: 2667 bp
ATGGTGGAAGGAAGGCTTTGTTTGTCCAAAGAGGCCAAAACTGGATTAGAAATTCTGAAGCGTACAAGGCTTCAGCGAATGAGACCGGAATCTGCTACCGAGCCTTTAAATCTCATTAATACAATGGCTCGAAGTGGAGGTGATGCTTTAAAATCTTCTGTATCCTGTGGTCTTACATTCCAGGGAGGTGCCCACTCAGTCTCTCTGTTCAGTGGTGCTTCACATGAAAAAGATGTTTTTCCAAAGCGTAGGGTGGATAAGTTCGAAACAAGTGATCTAGAGTGGACTGAGAAAATTCCCGAATGCCCTGTGTACTATCCAGCAATGGAGGAGTTTGAGGATCCTTTGGTTTATTTACAGAAGATAGCTCCAGAAGCTTCAAAATATGGTATATGCAAGATTGTTTCTCCTTTGAGTACTTCTACTCCAGCTGGGGTTGTATTGATGAGGGAGAAAGCAGGATTCAAGTTCACAACTAGAGTACAACCTCTTCGTCTTGCAGAGTGGGACAATGATGACAAGGTCACCTTTTTCATGAGCGGAAGAAATTATACATTCCGTGATTTCGAGAAAATGGCGAACAAGGTTTTTGCTCGTAGATATTATAGTACTGGTTCCCTTCCTGCCACGTACTTGGAAAAAGAATTTTGGCAAGAAATTTCTTGTGGAAAGACAGAAACTGTTGAGTATGCTTGTGATGTAGATGGTAGTGCCTTTTCATCTTCTCACAGCGATCCTCTTGGAAATAGCAAGTGGAATTTAAAGAATCTTTCAAGGTTACCCAAGTCCATTTTGCGTCTTCTAGAAATTGCAATTCCGGGTGTAACGGATCCCATGCTATACATTGGAATGCTGTTTAGTATGTTTGCTTGGCACGTGGAGGATCATTATTTGTACAGTATTAATTATCATCATTGCGGCGCATCCAAAACCTGGTATGGCATTCCAGGTGAAGCTGCTCTGCAATTTGAAAAGGTTGTTAGGGAGCATGTGTACACGCATGATATCATATCAACTGATGGAGAAGATGGAGCATTTGATGTCCTATTGGGGAAAACAACATTGTTTCCTCCAAATATTTTGTTGGAACATGATGTCCCTGTCTACAAGGCTGTGCAAAAGCCAGGAGAATTTGTTATTACTTTCCCCAGAGCATACCATGCTGGATTCAGTCATGGTTTCAACTGTGGCGAGGCAGTCAACTTTGCAATTGGTGATTGGTTCCCTTTAGGTGCTATTGCTAGCCGACGTTATGCGCTGCTTAACAGGATGCCTCTGCTTCCTCATGAAGAACTTCTGTGTAAAGAAGCGATGCTTCTACACACAAGTTTAGAACTTGAAGATTCAGACTATTCATCTGCTGATTTGGTCTCCCATAATAGAATTAAGTTGTCTTTTGTGAGATTGATGCGCTTTCAGCATCGTGCTCGTTGGTCTTTAATGAAGTCAGGGGCATGCACCGCTGTTTTGCCAAACTCCTATGGAACTGTTCTATGCAGCCTATGCAAGCGCGATTGCTATGTAGCTTACAATAATTGCACTTGTTACATGCATCCTGTCTGCCTGCGCCATGATGTAAGGTCTCTTGACTTCTCATGTGGGAGCAGCAATCTTACTCTCTTTTTAAGGGAGGAAATAACAGAACTGGAAGCTGCAGCCAGAAAATGTGAAAAGGACGATAGTATGCTGGAGGCGATAAGGGGGCTAGCGGAAAGTGGAGATGACTTGTATTCTTATCCATTAAATTTATCCCTGAGTGCTGAAGACAATGGATACTCTCCCTATTGTGAGATAAAATTTGAGTTGAACCCTGAACTTACTGGTAGAACTCAGTATAGGTCACATGAGCCAGAAACTGGTTCTCATGGCGGGCCCATGTTGAGATCTGATGCAGATTATTCAAGTCCTGCAGTGTCAGAAGGTTCCCTTTCATGTGCCGCATCAACTCTTTGTTCTCTTTTAGAGCCTCGGGAGAGCTTATCTGCTCCCAATAATGTACAGGGAAATGCTAACTCTTACACAGGGACTCTAAGTTCTAAAAGGCGTTTTGAAGAGTTGGTGCGCGGTGCATATGAGTCTTCTCAATCCTCTCTATCCTATGATGAATACTCTAGTGCACGTCCAGGGAATCTAAACGGATCAGAGGTTAGGCATGTTGTTGATCAAGGCAGTGATGATTCTGATTCAGAGATCTTTAGGGTTAAGCGTCGTTCATCATTGAAGACGGTGGACAAAAGAAGTGTAAATGATGTCTTGTCATCAAATCATTCCGAAAACAAGGGTTTTAAACGATTGAAGAAACTGCAACCTGACAGAAGATGCGGGCGATCGTCGGTGCCATTAGATTATTGCAGCCCTGGTGAACCAAATGCTAAATTTATTGCAACTAGTAATTACAAAGGAGTTCCAGAGAGTGCTTCTTTGGAGGGCAGGTTCTCCACAGGAAGTAGCGCTCCTACAGGAAGTAACGTTCCCATCTCTATCAAGTTTAAGAAGTTGGCCAATGAAGATGCAGCTAGACAACAGCGAGAACGCCACAGAAAGGATGGATACGAGGTTGAATTGGGAAAGAGCCGGAGGCAACCAACCCCAATGGAGATTGGGGCAAAGCGCCTTAAAGTCAAAGCCCCGTCGTTCCTAGGGTCGGAGAGCAGCAGGTTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0000792 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010016 GO:0010033 GO:0010228 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032454 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045815 GO:0048366 GO:0048367 GO:0048439 GO:0048518 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

889

Amino Acids

99.22

Weight (kDa)

6.91

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 103 - 135 1.6e-15 jmjN domain
JmjC PF02373 279 - 402 8.8e-40 JmjC domain, hydroxylase
zf-C5HC2 PF02928 499 - 551 5.1e-08 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2421, 2646
AccB1I GGYRCC 2 cut(s) 188, 2335
AccB7I CCANNNNNTGG 2 cut(s) 933, 1856
AccBSI CCGCTC 1 cut(s) 540
AccI GTMKAC 1 cut(s) 1104
AccII CGCG 2 cut(s) 1512, 2064
AciI CCGC 8 cut(s) 540, 918, 1461, 1709, 1869, 1938, 2064, 2322
AclI AACGTT 1 cut(s) 2469
AclWI GGATC 7 cut(s) 335, 348, 728, 824, 837, 891, 2146
AcoI YGGCCR 1 cut(s) 2499
AcsI RAATTY 9 cut(s) 51, 294, 644, 657, 757, 1130, 1744, 1796, 2375
AcuI CTGAAG 3 cut(s) 56, 77, 1782
AcvI CACGTG 1 cut(s) 877
AfaI GTAC 9 cut(s) 64, 314, 418, 483, 610, 632, 896, 995, 1998
AfeI AGCGCT 1 cut(s) 2452
AfiI CCNNNNNNNGG 4 cut(s) 933, 1856, 2542, 2644
AhlI ACTAGT 1 cut(s) 2387
AjiI CACGTC 1 cut(s) 2120
AjnI CCWGG 6 cut(s) 180, 932, 946, 1123, 2122, 2356
AloI GAACNNNNNNTCC 8 cut(s) 1080, 1112, 2017, 2049, 2417, 2449, 2454, 2486
AluBI AGCT 8 cut(s) 368, 377, 431, 956, 1526, 1652, 1977, 2516
AluI AGCT 8 cut(s) 368, 377, 431, 956, 1526, 1652, 1977, 2516
Alw21I GWGCWC 2 cut(s) 1429, 2119
Alw26I GTCTC 4 cut(s) 76, 205, 1375, 1587
Alw44I GTGCAC 1 cut(s) 2115
AlwI GGATC 7 cut(s) 335, 348, 728, 824, 837, 891, 2146
AlwNI CAGNNNCTG 2 cut(s) 680, 1856
Ama87I CYCGRG 1 cut(s) 1968
Aor51HI AGCGCT 1 cut(s) 2452
AoxI GGCC 3 cut(s) 33, 1871, 2499
ApaI GGGCCC 1 cut(s) 1875
ApaLI GTGCAC 1 cut(s) 2115
ApeKI GCWGC 9 cut(s) 956, 1258, 1497, 1605, 1652, 1655, 2352, 2513, 2652
ApoI RAATTY 9 cut(s) 51, 294, 644, 657, 757, 1130, 1744, 1796, 2375
AseI ATTAAT 2 cut(s) 117, 903
Asp700I GAANNNNTTC 1 cut(s) 1293
AspA2I CCTAGG 1 cut(s) 2638
AspLEI GCGC 8 cut(s) 923, 1258, 1413, 1512, 1569, 2064, 2453, 2613
AspS9I GGNCC 2 cut(s) 1871, 1872
AsuC2I CCSGG 1 cut(s) 819
AsuHPI GGTGA 5 cut(s) 152, 517, 962, 1226, 2372
AsuII TTCGAA 1 cut(s) 264
AsuNHI GCTAGC 2 cut(s) 1241, 1705
AvaI CYCGRG 1 cut(s) 1968
AvrII CCTAGG 1 cut(s) 2638
BaeGI GKGCMC 3 cut(s) 193, 1875, 2119
BalI TGGCCA 1 cut(s) 2501
BamHI GGATCC 2 cut(s) 340, 829
BanI GGYRCC 2 cut(s) 188, 2335
BanII GRGCYC 1 cut(s) 1875
BarI GAAGNNNNNNTAC 4 cut(s) 474, 506, 535, 567
BbrPI CACGTG 1 cut(s) 877
BbsI GAAGAC 4 cut(s) 791, 1770, 2070, 2228
Bbv12I GWGCWC 2 cut(s) 1429, 2119
BbvI GCAGC 8 cut(s) 943, 1245, 1509, 1617, 1639, 1667, 2364, 2525
BccI CCATC 5 cut(s) 698, 1013, 1022, 2483, 2540
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 6 cut(s) 182, 934, 948, 1125, 2124, 2358
BciVI GTATCC 3 cut(s) 172, 1766, 2543
BclI TGATCA 1 cut(s) 2161
BcnI CCSGG 1 cut(s) 819
BcoDI GTCTC 4 cut(s) 76, 205, 1375, 1587
BcuI ACTAGT 1 cut(s) 2387
BfaI CTAG 9 cut(s) 278, 477, 803, 1242, 1706, 2112, 2388, 2517, 2639
BfmI CTRYAG 3 cut(s) 1653, 1908, 2457
BfoI RGCGCY 2 cut(s) 2454, 2614
BfuAI ACCTGC 2 cut(s) 2421, 2646
BfuI GTATCC 3 cut(s) 172, 1766, 2543
BglII AGATCT 2 cut(s) 1883, 2190
BlnI CCTAGG 1 cut(s) 2638
BmcAI AGTACT 2 cut(s) 418, 610
Bme1390I CCNGG 7 cut(s) 182, 819, 934, 948, 1125, 2124, 2358
BmeT110I CYCGRG 1 cut(s) 1968
BmgBI CACGTC 1 cut(s) 2120
BmgT120I GGNCC 2 cut(s) 1871, 1872
BmiI GGNNCC 8 cut(s) 190, 342, 616, 831, 1223, 1873, 1924, 2337
BmrFI CCNGG 7 cut(s) 182, 819, 934, 948, 1125, 2124, 2358
BmtI GCTAGC 2 cut(s) 1245, 1709
BpiI GAAGAC 4 cut(s) 791, 1770, 2070, 2228
BpmI CTGGAG 3 cut(s) 354, 411, 1709
Bpu14I TTCGAA 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 819
BsaAI YACGTR 2 cut(s) 630, 877
BsaI GGTCTC 3 cut(s) 76, 1375, 1587
BsaJI CCNNGG 5 cut(s) 181, 1967, 2123, 2356, 2638
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 8 cut(s) 409, 439, 977, 1007, 1119, 1149, 2417, 2447
Bsc4I CCNNNNNNNGG 4 cut(s) 933, 1856, 2542, 2644
Bse1I ACTGG 5 cut(s) 46, 616, 1650, 1825, 1861
Bse3DI GCAATG 2 cut(s) 330, 913
BseBI CCWGG 6 cut(s) 182, 934, 948, 1125, 2124, 2358
BseDI CCNNGG 5 cut(s) 181, 1967, 2123, 2356, 2638
BseGI GGATG 4 cut(s) 923, 1275, 1552, 2551
BseLI CCNNNNNNNGG 4 cut(s) 933, 1856, 2542, 2644
BseMI GCAATG 2 cut(s) 330, 913
BseMII CTCAG 4 cut(s) 210, 279, 1745, 1844
BseNI ACTGG 5 cut(s) 46, 616, 1650, 1825, 1861
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 3 cut(s) 193, 1875, 2119
BseXI GCAGC 8 cut(s) 943, 1245, 1509, 1617, 1639, 1667, 2364, 2525
BseYI CCCAGC 1 cut(s) 431
Bsh1236I CGCG 2 cut(s) 1512, 2064
Bsh1285I CGRYCG 1 cut(s) 2330
BshFI GGCC 3 cut(s) 35, 1873, 2501
BshNI GGYRCC 2 cut(s) 188, 2335
BsiEI CGRYCG 1 cut(s) 2330
BsiHKAI GWGCWC 2 cut(s) 1429, 2119
BsiHKCI CYCGRG 1 cut(s) 1968
BsiSI CCGG 3 cut(s) 86, 818, 2575
BslFI GGGAC 3 cut(s) 521, 1082, 2038
BslI CCNNNNNNNGG 4 cut(s) 933, 1856, 2542, 2644
BsmAI GTCTC 4 cut(s) 76, 205, 1375, 1587
BsmFI GGGAC 3 cut(s) 521, 1082, 2038
BsmI GAATGC 3 cut(s) 308, 855, 942
BsnI GGCC 3 cut(s) 35, 1873, 2501
Bso31I GGTCTC 3 cut(s) 76, 1375, 1587
BsoBI CYCGRG 1 cut(s) 1968
Bsp119I TTCGAA 1 cut(s) 264
Bsp120I GGGCCC 1 cut(s) 1871
Bsp1286I GDGCHC 4 cut(s) 193, 1429, 1875, 2119
Bsp1407I TGTACA 3 cut(s) 894, 993, 1996
BspACI CCGC 8 cut(s) 540, 918, 1461, 1709, 1869, 1938, 2064, 2322
BspANI GGCC 3 cut(s) 35, 1873, 2501
BspCNI CTCAG 4 cut(s) 209, 280, 1746, 1843
BspFNI CGCG 2 cut(s) 1512, 2064
BspHI TCATGA 2 cut(s) 534, 1285
BspLI GGNNCC 8 cut(s) 190, 342, 616, 831, 1223, 1873, 1924, 2337
BspMAI CTGCAG 2 cut(s) 1657, 1912
BspMI ACCTGC 2 cut(s) 2421, 2646
BspOI GCTAGC 2 cut(s) 1245, 1709
BspPI GGATC 7 cut(s) 335, 348, 728, 824, 837, 891, 2146
BspT104I TTCGAA 1 cut(s) 264
BspT107I GGYRCC 2 cut(s) 188, 2335
BspTNI GGTCTC 3 cut(s) 76, 1375, 1587
BsrBI CCGCTC 1 cut(s) 540
BsrDI GCAATG 2 cut(s) 330, 913
BsrGI TGTACA 3 cut(s) 894, 993, 1996
BsrI ACTGG 5 cut(s) 46, 616, 1650, 1825, 1861
BssECI CCNNGG 5 cut(s) 181, 1967, 2123, 2356, 2638
BssT1I CCWWGG 1 cut(s) 2638
Bst2UI CCWGG 6 cut(s) 182, 934, 948, 1125, 2124, 2358
Bst4CI ACNGT 5 cut(s) 682, 899, 1187, 1489, 2227
Bst6I CTCTTC 2 cut(s) 495, 2046
BstAPI GCANNNNNTGC 2 cut(s) 1160, 1503
BstAUI TGTACA 3 cut(s) 894, 993, 1996
BstBAI YACGTR 2 cut(s) 630, 877
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 7 cut(s) 1243, 1456, 1508, 1565, 1707, 1871, 2324
BstDEI CTNAG 5 cut(s) 196, 288, 1754, 1830, 2030
BstEII GGTNACC 2 cut(s) 523, 777
BstF5I GGATG 4 cut(s) 923, 1275, 1552, 2551
BstFNI CGCG 2 cut(s) 1512, 2064
BstH2I RGCGCY 2 cut(s) 2454, 2614
BstHHI GCGC 8 cut(s) 923, 1258, 1413, 1512, 1569, 2064, 2453, 2613
BstMAI GTCTC 4 cut(s) 76, 205, 1375, 1587
BstMCI CGRYCG 1 cut(s) 2330
BstMWI GCNNNNNNNGC 6 cut(s) 67, 374, 1160, 1417, 1460, 1503
BstNI CCWGG 6 cut(s) 182, 934, 948, 1125, 2124, 2358
BstNSI RCATGY 4 cut(s) 992, 1458, 1552, 2156
BstPI GGTNACC 2 cut(s) 523, 777
BstSCI CCNGG 7 cut(s) 180, 817, 932, 946, 1123, 2122, 2356
BstSFI CTRYAG 3 cut(s) 1653, 1908, 2457
BstSLI GKGCMC 3 cut(s) 193, 1875, 2119
BstUI CGCG 2 cut(s) 1512, 2064
BstV1I GCAGC 8 cut(s) 943, 1245, 1509, 1617, 1639, 1667, 2364, 2525
BstV2I GAAGAC 4 cut(s) 791, 1770, 2070, 2228
BstX2I RGATCY 4 cut(s) 340, 829, 1883, 2190
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 4 cut(s) 340, 829, 1883, 2190
BsuI GTATCC 3 cut(s) 172, 1766, 2543
BsuRI GGCC 3 cut(s) 35, 1873, 2501
BtgZI GCGATG 1 cut(s) 1325
BtrI CACGTC 1 cut(s) 2120
BtsCI GGATG 4 cut(s) 923, 1275, 1552, 2551
BtsI GCAGTG 2 cut(s) 1917, 2176
BtsIMutI CAGTG 3 cut(s) 217, 1917, 2176
BveI ACCTGC 2 cut(s) 2421, 2646
Cac8I GCNNGC 7 cut(s) 1243, 1456, 1508, 1565, 1707, 1871, 2324
CaiI CAGNNNCTG 2 cut(s) 680, 1856
CciI TCATGA 2 cut(s) 534, 1285
CfoI GCGC 8 cut(s) 923, 1258, 1413, 1512, 1569, 2064, 2453, 2613
Cfr13I GGNCC 2 cut(s) 1871, 1872
CseI GACGC 2 cut(s) 785, 2195
CsiI ACCWGGT 1 cut(s) 932
Csp6I GTAC 9 cut(s) 63, 313, 417, 482, 609, 631, 895, 994, 1997
CviQI GTAC 9 cut(s) 63, 313, 417, 482, 609, 631, 895, 994, 1997
DdeI CTNAG 5 cut(s) 196, 288, 1754, 1830, 2030
DraI TTTAAA 4 cut(s) 108, 150, 762, 2287
EaeI YGGCCR 1 cut(s) 2499
Eam1104I CTCTTC 2 cut(s) 495, 2046
EarI CTCTTC 2 cut(s) 495, 2046
Eco130I CCWWGG 1 cut(s) 2638
Eco24I GRGCYC 1 cut(s) 1875
Eco31I GGTCTC 3 cut(s) 76, 1375, 1587
Eco32I GATATC 1 cut(s) 1006
Eco47III AGCGCT 1 cut(s) 2452
Eco57I CTGAAG 3 cut(s) 56, 77, 1782
Eco72I CACGTG 1 cut(s) 877
Eco88I CYCGRG 1 cut(s) 1968
Eco91I GGTNACC 2 cut(s) 523, 777
EcoO65I GGTNACC 2 cut(s) 523, 777
EcoRII CCWGG 6 cut(s) 180, 932, 946, 1123, 2122, 2356
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 2638
EcoT22I ATGCAT 1 cut(s) 1554
EcoT38I GRGCYC 1 cut(s) 1875
ErhI CCWWGG 1 cut(s) 2638
FalI AAGNNNNNCTT 7 cut(s) 32, 512, 544, 1299, 1331, 1911, 1943
FaqI GGGAC 3 cut(s) 521, 1082, 2038
FauI CCCGC 2 cut(s) 1862, 2315
FauNDI CATATG 1 cut(s) 2071
FbaI TGATCA 1 cut(s) 2161
FblI GTMKAC 1 cut(s) 1104
FokI GGATG 4 cut(s) 910, 1282, 1539, 2558
FriOI GRGCYC 1 cut(s) 1875
FspBI CTAG 9 cut(s) 278, 477, 803, 1242, 1706, 2112, 2388, 2517, 2639
GlaI GCGC 8 cut(s) 922, 1257, 1412, 1511, 1568, 2063, 2452, 2612
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 3 cut(s) 354, 411, 1709
HaeII RGCGCY 2 cut(s) 2454, 2614
HaeIII GGCC 3 cut(s) 35, 1873, 2501
HapII CCGG 3 cut(s) 86, 818, 2575
HgaI GACGC 2 cut(s) 785, 2195
HhaI GCGC 8 cut(s) 923, 1258, 1413, 1512, 1569, 2064, 2453, 2613
Hin6I GCGC 8 cut(s) 921, 1256, 1411, 1510, 1567, 2062, 2451, 2611
HinP1I GCGC 8 cut(s) 921, 1256, 1411, 1510, 1567, 2062, 2451, 2611
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 1 cut(s) 375
HpaII CCGG 3 cut(s) 86, 818, 2575
HphI GGTGA 5 cut(s) 152, 517, 962, 1226, 2372
Hpy188I TCNGA 9 cut(s) 57, 1348, 1888, 1918, 2143, 2182, 2188, 2272, 2647
Hpy99I CGWCG 4 cut(s) 1251, 2211, 2335, 2635
HpyAV CCTTC 3 cut(s) 6, 629, 1913
HpyCH4III ACNGT 5 cut(s) 682, 899, 1187, 1489, 2227
HpyCH4IV ACGT 5 cut(s) 629, 876, 1249, 2119, 2469
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 374, 1160, 1417, 1460, 1503
HpyF3I CTNAG 5 cut(s) 196, 288, 1754, 1830, 2030
HpySE526I ACGT 5 cut(s) 629, 876, 1249, 2119, 2469
HspAI GCGC 8 cut(s) 921, 1256, 1411, 1510, 1567, 2062, 2451, 2611
Ksp22I TGATCA 1 cut(s) 2161
LmnI GCTCC 6 cut(s) 373, 985, 1031, 1602, 1990, 2458
Lsp1109I GCAGC 8 cut(s) 943, 1245, 1509, 1617, 1639, 1667, 2364, 2525
MabI ACCWGGT 1 cut(s) 932
MaeI CTAG 9 cut(s) 278, 477, 803, 1242, 1706, 2112, 2388, 2517, 2639
MaeII ACGT 5 cut(s) 629, 876, 1249, 2119, 2469
MaeIII GTNAC 6 cut(s) 523, 777, 823, 1544, 1839, 2465
MbiI CCGCTC 1 cut(s) 540
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 4 cut(s) 340, 829, 1883, 2190
MhlI GDGCHC 4 cut(s) 193, 1429, 1875, 2119
MlsI TGGCCA 1 cut(s) 2501
MluNI TGGCCA 1 cut(s) 2501
MlyI GAGTC 2 cut(s) 2020, 2084
MmeI TCCRAC 2 cut(s) 1065, 2625
Mox20I TGGCCA 1 cut(s) 2501
Mph1103I ATGCAT 1 cut(s) 1554
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 1 cut(s) 2501
MslI CAYNNNNRTG 1 cut(s) 848
Msp20I TGGCCA 1 cut(s) 2501
MspA1I CMGCKG 2 cut(s) 431, 1463
MspI CCGG 3 cut(s) 86, 818, 2575
MspR9I CCNGG 7 cut(s) 182, 819, 934, 948, 1125, 2124, 2358
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 3 cut(s) 308, 855, 942
MvaI CCWGG 6 cut(s) 182, 934, 948, 1125, 2124, 2358
MvnI CGCG 2 cut(s) 1512, 2064
MwoI GCNNNNNNNGC 6 cut(s) 67, 374, 1160, 1417, 1460, 1503
NciI CCSGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 2071
NheI GCTAGC 2 cut(s) 1241, 1705
NlaIV GGNNCC 8 cut(s) 190, 342, 616, 831, 1223, 1873, 1924, 2337
NmuCI GTSAC 2 cut(s) 523, 1839
NsiI ATGCAT 1 cut(s) 1554
NspI RCATGY 4 cut(s) 992, 1458, 1552, 2156
NspV TTCGAA 1 cut(s) 264
PaeI GCATGC 1 cut(s) 1458
PagI TCATGA 2 cut(s) 534, 1285
PctI GAATGC 3 cut(s) 308, 855, 942
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 8 cut(s) 89, 462, 766, 1167, 1343, 2128, 2177, 2183
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 2 cut(s) 933, 1856
Ple19I CGATCG 1 cut(s) 2330
PleI GAGTC 2 cut(s) 2020, 2083
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 2 cut(s) 2020, 2083
Ppu21I YACGTR 2 cut(s) 630, 877
PshBI ATTAAT 2 cut(s) 117, 903
Psp1406I AACGTT 1 cut(s) 2469
Psp6I CCWGG 6 cut(s) 180, 932, 946, 1123, 2122, 2356
PspCI CACGTG 1 cut(s) 877
PspEI GGTNACC 2 cut(s) 523, 777
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 6 cut(s) 180, 932, 946, 1123, 2122, 2356
PspN4I GGNNCC 8 cut(s) 190, 342, 616, 831, 1223, 1873, 1924, 2337
PspOMI GGGCCC 1 cut(s) 1871
PspPI GGNCC 2 cut(s) 1871, 1872
PstI CTGCAG 2 cut(s) 1657, 1912
PstNI CAGNNNCTG 2 cut(s) 680, 1856
PsuI RGATCY 4 cut(s) 340, 829, 1883, 2190
PsyI GACNNNGTC 1 cut(s) 521
PvuI CGATCG 1 cut(s) 2330
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 9 cut(s) 64, 314, 418, 483, 610, 632, 896, 995, 1998
RsaNI GTAC 9 cut(s) 63, 313, 417, 482, 609, 631, 895, 994, 1997
RseI CAYNNNNRTG 1 cut(s) 848
Sau96I GGNCC 2 cut(s) 1871, 1872
ScaI AGTACT 2 cut(s) 418, 610
SchI GAGTC 2 cut(s) 2020, 2084
ScrFI CCNGG 7 cut(s) 182, 819, 934, 948, 1125, 2124, 2358
SduI GDGCHC 4 cut(s) 193, 1429, 1875, 2119
SexAI ACCWGGT 1 cut(s) 932
SfcI CTRYAG 3 cut(s) 1653, 1908, 2457
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 848
SpeI ACTAGT 1 cut(s) 2387
SphI GCATGC 1 cut(s) 1458
SsiI CCGC 8 cut(s) 540, 918, 1461, 1709, 1869, 1938, 2064, 2322
SspI AATATT 1 cut(s) 1078
SspMI CTAG 9 cut(s) 278, 477, 803, 1242, 1706, 2112, 2388, 2517, 2639
StyD4I CCNGG 7 cut(s) 180, 817, 932, 946, 1123, 2122, 2356
StyI CCWWGG 1 cut(s) 2638
TaaI ACNGT 5 cut(s) 682, 899, 1187, 1489, 2227
TaiI ACGT 5 cut(s) 632, 879, 1252, 2122, 2472
TaqI TCGA 3 cut(s) 130, 264, 567
TatI WGTACW 7 cut(s) 312, 416, 481, 608, 894, 993, 1996
TauI GCSGC 2 cut(s) 921, 1940
TfiI GAWTC 8 cut(s) 89, 462, 766, 1167, 1343, 2128, 2177, 2183
TscAI CASTG 3 cut(s) 217, 1917, 2176
TseFI GTSAC 2 cut(s) 523, 1839
TseI GCWGC 9 cut(s) 956, 1258, 1497, 1605, 1652, 1655, 2352, 2513, 2652
Tsp45I GTSAC 2 cut(s) 523, 1839
TspGWI ACGGA 3 cut(s) 548, 842, 2151
TspRI CASTG 3 cut(s) 217, 1917, 2176
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 2 cut(s) 933, 1856
VneI GTGCAC 1 cut(s) 2115
VspI ATTAAT 2 cut(s) 117, 903
XapI RAATTY 9 cut(s) 51, 294, 644, 657, 757, 1130, 1744, 1796, 2375
XbaI TCTAGA 2 cut(s) 277, 802
XceI RCATGY 4 cut(s) 992, 1458, 1552, 2156
XcmI CCANNNNNNNNNTGG 1 cut(s) 2543
XmaJI CCTAGG 1 cut(s) 2638
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 9 cut(s) 278, 477, 803, 1242, 1706, 2112, 2388, 2517, 2639
ZrmI AGTACT 2 cut(s) 418, 610
Zsp2I ATGCAT 1 cut(s) 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.