pycom16g25030

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007240
Physical Location & Seq
Reverse (-)
26731749 .. 26737388
5640 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g25030.4

Sequence Viewer

Length: 2526 bp
ATGGTGGAAGGAAGGGTTTGTTTGTCCAAAGAGGCCAAAACTGGATTAGAAATTCTGAAGCGTACAAGGCTTCAGCGAATGAGACCGCAAACTTCTACCGAGCCTGTAAATCTCATTAATACAATGGCTCGAAGTGGAGGCGATGCTTTAGAATCTTCTGTATCCTGTGGTCTTAGATTACAGGGAGGTGCCAACTCAGTTTCTCTCTTCACTGGTGCTTCACATGAAAAAGATGTTTTTCCAAAGCGTAGGGTGGATAAGTTCGAAACAAGTGACCTAGAGTGGACTGAGAAAATTCCCGAATGCCCTGTCTACTATCCAGCAATGGAGGAGTTTGAGGATCCTTTGGTTTATTTACAGAAGATAGCTCCAGAAGCTTCAAAATATGGTATATGCAAGATTGTTTCTCCCTTGAGTACTTCTACTCCAGCTGGGGTTGTATTAATGAGGGAGAAAGCAGGATTCAAGTTCACAACTAGAGTACAACCTCTTCGTCTTGCAGAGTGGGACAATGATGACAAGGTCACCTTTTTCATGAGCGGAAGAAATTATACATTCCGTGATTTCGAGAAAATGGCGAACAAGGTTTTTGCTCGTAGATATTATAGTACTGGTTCCCTCCCCGCCACATACTTGGAAAAAGAATTTTGGCAAGAAATTTCTTGTGGAAAGACAGAAACTGTTGAGTATGCTTGTGATGTAGATGGTAGTGCCTTTTCATCTTCTCGCAGTGATCCTCTTGGAAATAGCAAGTGGAATTTAAAGAATCTTTCAAGGTTACCCAAGTCCATTTTGCGTCTTCTAGAAATTGCAATTCCGGGTGTAACGGATCGCATGCTATACATTGGAATGCTGTTTAGTATGTTTGCTTGGCATGTGGACGATCATTATTTGTACAGTATTAATTATCATCATTGCGGCGCATCCAAAACCTGGTATGGCATTCCAGGTAAAGCTGCTCTGCAATTTGAAAAGGTTGTTAGGGAGCATGTGTACACGCATGATATCATATCAACTGATGGAGAAGATGGAGCAGTTGATGTCCTATTGGGGAAAACAACATTGTTTCCTCCAAATATTTTGTTGGAACATGATGTCCCTGTCTACAAGGCTGTGCAAAAGCCAGGAGAATTTGTTATTACTTTCCCCAGAGCATACCATGCTGGATTCAGTCATGGTTTCAACTGTGGCGAGGCAGTCAACTTTGCAATTGGTGATTGGTTCCCTTTAGGTGCTATTGCTAGCCAACGTTACGCGCTGCTTAACAGGATGCCTATGCTTCCTCATGAAGAACTTCTGTGTAAAGAAGCGATGCTTCTACACACAAGTTTAGAACTTGAAGATTCAGACTGTTCATCTGCTGATTTGGTCTCCCATAATAGAATTAAGTTGTCTTTTGTGAGATTGATGCGCTTTCAGCATTGTGCTCGTTGGTCTTTAATGAAGTCAGGGGCATGCACTGCTATTTTGCCAAACTCCTATGGAACTGTTCTATGCAGCCTATGCAAGCGCGATTGCTATGTAGCTTACATTAATTGCACTTGTTACATGCATCCTGTCTGCCTGCGCCATGATGTAAGGTCTCTTGACTTCTCATGTGGGAGCAATCCTACTCTCTTTTTAAGGGAGGAAATAACAGAACTGGAAGCTGCAGCCAGAAAATTTGAAAAGGACGATAGTATGCTGGAGGCGATAAGGGGGCTAGTGGAAAGTGGAGATGACTTGTATTCTTATCCATTAAATTTATCCCTGAGTGCTGAAGACAAAGGATACTCTCCCTATTGTGAGATAAAATTTGAGTTGAACCCTGAACTTACTGGTAGAACTTGGTATCGGTCACAAGAGCCAGAAACTGGTTCTCATGGCCAGCCCATGTTGAGATCTGATGCAGATTATTCAAGTCCGGCAGTGTCAGAAGGTTCCCTTTCATGTGCCGCATCAACTCTTTGTTCCCTTTTAGAGCCTCGGGAGAGCTTATCTGCACCCAATCATGTACAGGGAAATGCTAACTCTTACACAGGGACTCTAAGTTCTAAAAGGCGTTTTGAAGAGTTGGTGCGTGGTGCATATGAGTCTTCTCAATCCTCTGTATCTTATGATGAATACTCTAGTGTGCGTCCAGGGAATCTAAACGGATCAGAGGTTAGGCATGTTGTTGATCAAGGAAGTGACGATTCTGATTCAGAGATCTTTAGGGTTAAGCGCCGTTCATCATTGAAGGTGGTGGATAAAAGAAGTGTAAATGATGTCTTGTCATCAAATCATTCCGAAAACAAGGGTTTTAAACGATTGAGGAAACTGCAACCTGACAGAAGATGCGGGCGATCATTGGTGCCATTAGATTATTGCAGCCCTGGTGAACCAAATGCTAAATTTATTGCAACTAGTAATTACAAAGGAGTTCCAGAGAGTGCTTCTTTGGAGGGCAGGTTCTCCACAGGAAGTAGCGCTCCTACAGGAAGTAACGTTCCCATCTCTATCAAGCTTAAGAAGTTGGCCAATGAAGATTCAGCTAGACAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0000792 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010016 GO:0010033 GO:0010228 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032454 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045815 GO:0048366 GO:0048367 GO:0048439 GO:0048518 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

842

Amino Acids

94.04

Weight (kDa)

6.5

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2418
AccB1I GGYRCC 2 cut(s) 188, 2332
AccB7I CCANNNNNTGG 2 cut(s) 933, 1853
AccBSI CCGCTC 1 cut(s) 540
AccI GTMKAC 2 cut(s) 312, 1104
AccII CGCG 2 cut(s) 1256, 1512
AciI CCGC 6 cut(s) 86, 540, 624, 918, 1935, 2319
AclI AACGTT 2 cut(s) 1249, 2466
AclWI GGATC 5 cut(s) 335, 348, 728, 837, 2143
AcoI YGGCCR 2 cut(s) 1864, 2496
AcuI CTGAAG 3 cut(s) 56, 77, 1779
AfaI GTAC 7 cut(s) 64, 418, 483, 610, 896, 995, 1995
AfeI AGCGCT 1 cut(s) 2449
AfiI CCNNNNNNNGG 2 cut(s) 933, 1853
AflII CTTAAG 1 cut(s) 2486
AhlI ACTAGT 1 cut(s) 2384
AjnI CCWGG 5 cut(s) 932, 946, 1123, 2119, 2353
AloI GAACNNNNNNTCC 8 cut(s) 1080, 1112, 2014, 2046, 2414, 2446, 2451, 2483
AluBI AGCT 9 cut(s) 368, 377, 431, 956, 1526, 1649, 1974, 2485, 2513
AluI AGCT 9 cut(s) 368, 377, 431, 956, 1526, 1649, 1974, 2485, 2513
Alw21I GWGCWC 1 cut(s) 1429
Alw26I GTCTC 3 cut(s) 76, 1375, 1587
AlwI GGATC 5 cut(s) 335, 348, 728, 837, 2143
AlwNI CAGNNNCTG 2 cut(s) 680, 1853
Ama87I CYCGRG 1 cut(s) 1965
Aor51HI AGCGCT 1 cut(s) 2449
AoxI GGCC 3 cut(s) 33, 1864, 2496
ApeKI GCWGC 6 cut(s) 956, 1258, 1497, 1649, 1652, 2349
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AseI ATTAAT 4 cut(s) 117, 443, 903, 1533
Asp700I GAANNNNTTC 1 cut(s) 1293
AspLEI GCGC 7 cut(s) 923, 1258, 1413, 1512, 1569, 2205, 2450
AsuC2I CCSGG 1 cut(s) 819
AsuHPI GGTGA 3 cut(s) 517, 1226, 2369
AsuII TTCGAA 1 cut(s) 264
AsuNHI GCTAGC 1 cut(s) 1241
AvaI CYCGRG 1 cut(s) 1965
BalI TGGCCA 2 cut(s) 1866, 2498
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 188, 2332
BarI GAAGNNNNNNTAC 4 cut(s) 474, 506, 535, 567
BbsI GAAGAC 3 cut(s) 791, 1767, 2067
Bbv12I GWGCWC 1 cut(s) 1429
BbvI GCAGC 6 cut(s) 943, 1245, 1509, 1636, 1664, 2361
BccI CCATC 4 cut(s) 698, 1013, 1022, 2480
BceAI ACGGC 1 cut(s) 2190
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 5 cut(s) 934, 948, 1125, 2121, 2355
BciVI GTATCC 2 cut(s) 172, 1763
BclI TGATCA 1 cut(s) 2158
BcnI CCSGG 1 cut(s) 819
BcoDI GTCTC 3 cut(s) 76, 1375, 1587
BcuI ACTAGT 1 cut(s) 2384
BfaI CTAG 8 cut(s) 278, 477, 803, 1242, 1703, 2109, 2385, 2514
BfmI CTRYAG 2 cut(s) 1650, 2454
BfoI RGCGCY 2 cut(s) 2206, 2451
BfrI CTTAAG 1 cut(s) 2486
BfuAI ACCTGC 1 cut(s) 2418
BfuI GTATCC 2 cut(s) 172, 1763
BglII AGATCT 2 cut(s) 1880, 2187
BisI GCNGC 8 cut(s) 919, 957, 1259, 1498, 1650, 1653, 1935, 2350
BlsI GCNGC 8 cut(s) 920, 958, 1260, 1499, 1651, 1654, 1936, 2351
BmcAI AGTACT 2 cut(s) 418, 610
Bme1390I CCNGG 6 cut(s) 819, 934, 948, 1125, 2121, 2355
BmeT110I CYCGRG 1 cut(s) 1965
BmiI GGNNCC 6 cut(s) 190, 342, 616, 1223, 1921, 2334
BmrFI CCNGG 6 cut(s) 819, 934, 948, 1125, 2121, 2355
BmsI GCATC 9 cut(s) 133, 932, 1260, 1302, 1398, 1561, 1876, 1946, 2306
BmtI GCTAGC 1 cut(s) 1245
BpiI GAAGAC 3 cut(s) 791, 1767, 2067
BpmI CTGGAG 3 cut(s) 354, 411, 1706
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 1 cut(s) 819
BsaI GGTCTC 3 cut(s) 76, 1375, 1587
BsaJI CCNNGG 3 cut(s) 1964, 2120, 2353
BsaXI ACNNNNNCTCC 8 cut(s) 409, 439, 977, 1007, 1119, 1149, 2414, 2444
Bsc4I CCNNNNNNNGG 2 cut(s) 933, 1853
Bse1I ACTGG 6 cut(s) 46, 217, 616, 1647, 1822, 1858
Bse3DI GCAATG 2 cut(s) 330, 913
BseBI CCWGG 5 cut(s) 934, 948, 1125, 2121, 2355
BseDI CCNNGG 3 cut(s) 1964, 2120, 2353
BseGI GGATG 3 cut(s) 923, 1275, 1552
BseLI CCNNNNNNNGG 2 cut(s) 933, 1853
BseMI GCAATG 2 cut(s) 330, 913
BseMII CTCAG 3 cut(s) 210, 279, 1742
BseNI ACTGG 6 cut(s) 46, 217, 616, 1647, 1822, 1858
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 6 cut(s) 943, 1245, 1509, 1636, 1664, 2361
BseYI CCCAGC 1 cut(s) 431
BsgI GTGCAG 1 cut(s) 1965
Bsh1236I CGCG 2 cut(s) 1256, 1512
BshFI GGCC 3 cut(s) 35, 1866, 2498
BshNI GGYRCC 2 cut(s) 188, 2332
BsiHKAI GWGCWC 1 cut(s) 1429
BsiHKCI CYCGRG 1 cut(s) 1965
BsiSI CCGG 2 cut(s) 818, 1904
BslFI GGGAC 3 cut(s) 521, 1082, 2035
BslI CCNNNNNNNGG 2 cut(s) 933, 1853
BsmAI GTCTC 3 cut(s) 76, 1375, 1587
BsmFI GGGAC 3 cut(s) 521, 1082, 2035
BsmI GAATGC 3 cut(s) 308, 855, 942
BsnI GGCC 3 cut(s) 35, 1866, 2498
Bso31I GGTCTC 3 cut(s) 76, 1375, 1587
BsoBI CYCGRG 1 cut(s) 1965
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 1429
Bsp1407I TGTACA 3 cut(s) 894, 993, 1993
Bsp143I GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
BspACI CCGC 6 cut(s) 86, 540, 624, 918, 1935, 2319
BspANI GGCC 3 cut(s) 35, 1866, 2498
BspCNI CTCAG 3 cut(s) 209, 280, 1743
BspFNI CGCG 2 cut(s) 1256, 1512
BspHI TCATGA 2 cut(s) 534, 1285
BspLI GGNNCC 6 cut(s) 190, 342, 616, 1223, 1921, 2334
BspMAI CTGCAG 1 cut(s) 1654
BspMI ACCTGC 1 cut(s) 2418
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 5 cut(s) 335, 348, 728, 837, 2143
BspT104I TTCGAA 1 cut(s) 264
BspT107I GGYRCC 2 cut(s) 188, 2332
BspTI CTTAAG 1 cut(s) 2486
BspTNI GGTCTC 3 cut(s) 76, 1375, 1587
BsrBI CCGCTC 1 cut(s) 540
BsrDI GCAATG 2 cut(s) 330, 913
BsrGI TGTACA 3 cut(s) 894, 993, 1993
BsrI ACTGG 6 cut(s) 46, 217, 616, 1647, 1822, 1858
BssECI CCNNGG 3 cut(s) 1964, 2120, 2353
BssMI GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
Bst2UI CCWGG 5 cut(s) 934, 948, 1125, 2121, 2355
Bst4CI ACNGT 6 cut(s) 682, 899, 1187, 1352, 1489, 2523
Bst6I CTCTTC 3 cut(s) 212, 495, 2043
BstAFI CTTAAG 1 cut(s) 2486
BstAPI GCANNNNNTGC 3 cut(s) 1160, 1460, 1503
BstAUI TGTACA 3 cut(s) 894, 993, 1993
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 7 cut(s) 836, 1243, 1456, 1508, 1565, 1868, 2321
BstDEI CTNAG 5 cut(s) 173, 196, 288, 1751, 2027
BstEII GGTNACC 2 cut(s) 523, 777
BstF5I GGATG 3 cut(s) 923, 1275, 1552
BstFNI CGCG 2 cut(s) 1256, 1512
BstH2I RGCGCY 2 cut(s) 2206, 2451
BstHHI GCGC 7 cut(s) 923, 1258, 1413, 1512, 1569, 2205, 2450
BstKTI GATC 9 cut(s) 343, 736, 832, 886, 1883, 2138, 2161, 2190, 2327
BstMAI GTCTC 3 cut(s) 76, 1375, 1587
BstMBI GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
BstMWI GCNNNNNNNGC 6 cut(s) 67, 374, 1160, 1417, 1460, 1503
BstNI CCWGG 5 cut(s) 934, 948, 1125, 2121, 2355
BstNSI RCATGY 6 cut(s) 838, 878, 992, 1458, 1552, 2153
BstPI GGTNACC 2 cut(s) 523, 777
BstSCI CCNGG 6 cut(s) 817, 932, 946, 1123, 2119, 2353
BstSFI CTRYAG 2 cut(s) 1650, 2454
BstUI CGCG 2 cut(s) 1256, 1512
BstV1I GCAGC 6 cut(s) 943, 1245, 1509, 1636, 1664, 2361
BstV2I GAAGAC 3 cut(s) 791, 1767, 2067
BstX2I RGATCY 3 cut(s) 340, 1880, 2187
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 3 cut(s) 340, 1880, 2187
BsuI GTATCC 2 cut(s) 172, 1763
BsuRI GGCC 3 cut(s) 35, 1866, 2498
BtgZI GCGATG 2 cut(s) 156, 1325
BtsCI GGATG 3 cut(s) 923, 1275, 1552
BtsI GCAGTG 3 cut(s) 736, 1458, 1914
BtsIMutI CAGTG 4 cut(s) 210, 736, 1458, 1914
BveI ACCTGC 1 cut(s) 2418
Cac8I GCNNGC 7 cut(s) 836, 1243, 1456, 1508, 1565, 1868, 2321
CaiI CAGNNNCTG 2 cut(s) 680, 1853
CciI TCATGA 2 cut(s) 534, 1285
CfoI GCGC 7 cut(s) 923, 1258, 1413, 1512, 1569, 2205, 2450
CseI GACGC 2 cut(s) 785, 2105
CsiI ACCWGGT 1 cut(s) 932
Csp6I GTAC 7 cut(s) 63, 417, 482, 609, 895, 994, 1994
CviQI GTAC 7 cut(s) 63, 417, 482, 609, 895, 994, 1994
DdeI CTNAG 5 cut(s) 173, 196, 288, 1751, 2027
DpnI GATC 9 cut(s) 342, 735, 831, 885, 1882, 2137, 2160, 2189, 2326
DpnII GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
DraI TTTAAA 2 cut(s) 762, 2284
EaeI YGGCCR 2 cut(s) 1864, 2496
Eam1104I CTCTTC 3 cut(s) 212, 495, 2043
EarI CTCTTC 3 cut(s) 212, 495, 2043
Eco31I GGTCTC 3 cut(s) 76, 1375, 1587
Eco32I GATATC 1 cut(s) 1006
Eco47III AGCGCT 1 cut(s) 2449
Eco57I CTGAAG 3 cut(s) 56, 77, 1779
Eco88I CYCGRG 1 cut(s) 1965
Eco91I GGTNACC 2 cut(s) 523, 777
EcoO65I GGTNACC 2 cut(s) 523, 777
EcoRII CCWGG 5 cut(s) 932, 946, 1123, 2119, 2353
EcoRV GATATC 1 cut(s) 1006
EcoT22I ATGCAT 1 cut(s) 1554
FalI AAGNNNNNCTT 6 cut(s) 512, 544, 1299, 1331, 1908, 1940
FaqI GGGAC 3 cut(s) 521, 1082, 2035
FauI CCCGC 2 cut(s) 631, 2312
FauNDI CATATG 1 cut(s) 2068
FbaI TGATCA 1 cut(s) 2158
FblI GTMKAC 2 cut(s) 312, 1104
Fnu4HI GCNGC 8 cut(s) 919, 957, 1259, 1498, 1650, 1653, 1935, 2350
FokI GGATG 3 cut(s) 910, 1282, 1539
Fsp4HI GCNGC 8 cut(s) 919, 957, 1259, 1498, 1650, 1653, 1935, 2350
FspBI CTAG 8 cut(s) 278, 477, 803, 1242, 1703, 2109, 2385, 2514
GlaI GCGC 7 cut(s) 922, 1257, 1412, 1511, 1568, 2204, 2449
GluI GCNGC 8 cut(s) 919, 957, 1259, 1498, 1650, 1653, 1935, 2350
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 3 cut(s) 354, 411, 1706
HaeII RGCGCY 2 cut(s) 2206, 2451
HaeIII GGCC 3 cut(s) 35, 1866, 2498
HapII CCGG 2 cut(s) 818, 1904
HgaI GACGC 2 cut(s) 785, 2105
HhaI GCGC 7 cut(s) 923, 1258, 1413, 1512, 1569, 2205, 2450
Hin6I GCGC 7 cut(s) 921, 1256, 1411, 1510, 1567, 2203, 2448
HinP1I GCGC 7 cut(s) 921, 1256, 1411, 1510, 1567, 2203, 2448
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 2 cut(s) 375, 2483
HpaII CCGG 2 cut(s) 818, 1904
HphI GGTGA 3 cut(s) 517, 1226, 2369
Hpy166II GTNNAC 9 cut(s) 285, 313, 471, 880, 994, 996, 1105, 1201, 2360
Hpy188I TCNGA 8 cut(s) 57, 1348, 1885, 1915, 2140, 2179, 2185, 2269
Hpy188III TCNNGA 9 cut(s) 299, 371, 535, 568, 803, 1286, 1586, 1967, 2405
Hpy8I GTNNAC 9 cut(s) 285, 313, 471, 880, 994, 996, 1105, 1201, 2360
HpyAV CCTTC 3 cut(s) 6, 1910, 2212
HpyCH4III ACNGT 6 cut(s) 682, 899, 1187, 1352, 1489, 2523
HpyCH4IV ACGT 2 cut(s) 1249, 2466
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 374, 1160, 1417, 1460, 1503
HpyF3I CTNAG 5 cut(s) 173, 196, 288, 1751, 2027
HpySE526I ACGT 2 cut(s) 1249, 2466
HspAI GCGC 7 cut(s) 921, 1256, 1411, 1510, 1567, 2203, 2448
Ksp22I TGATCA 1 cut(s) 2158
Kzo9I GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
LmnI GCTCC 5 cut(s) 373, 985, 1031, 1602, 2455
Lsp1109I GCAGC 6 cut(s) 943, 1245, 1509, 1636, 1664, 2361
LweI GCATC 9 cut(s) 133, 932, 1260, 1302, 1398, 1561, 1876, 1946, 2306
MabI ACCWGGT 1 cut(s) 932
MaeI CTAG 8 cut(s) 278, 477, 803, 1242, 1703, 2109, 2385, 2514
MaeII ACGT 2 cut(s) 1249, 2466
MaeIII GTNAC 9 cut(s) 272, 523, 777, 823, 1250, 1544, 1836, 2168, 2462
MalI GATC 9 cut(s) 342, 735, 831, 885, 1882, 2137, 2160, 2189, 2326
MbiI CCGCTC 1 cut(s) 540
MboI GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 3 cut(s) 340, 1880, 2187
MhlI GDGCHC 1 cut(s) 1429
MlsI TGGCCA 2 cut(s) 1866, 2498
MluNI TGGCCA 2 cut(s) 1866, 2498
MlyI GAGTC 2 cut(s) 2017, 2081
MmeI TCCRAC 1 cut(s) 1065
Mox20I TGGCCA 2 cut(s) 1866, 2498
Mph1103I ATGCAT 1 cut(s) 1554
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 2 cut(s) 1866, 2498
MslI CAYNNNNRTG 1 cut(s) 848
Msp20I TGGCCA 2 cut(s) 1866, 2498
MspA1I CMGCKG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 2486
MspI CCGG 2 cut(s) 818, 1904
MspR9I CCNGG 6 cut(s) 819, 934, 948, 1125, 2121, 2355
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 3 cut(s) 308, 855, 942
MvaI CCWGG 5 cut(s) 934, 948, 1125, 2121, 2355
MvnI CGCG 2 cut(s) 1256, 1512
MwoI GCNNNNNNNGC 6 cut(s) 67, 374, 1160, 1417, 1460, 1503
NciI CCSGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 2068
NdeII GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
NheI GCTAGC 1 cut(s) 1241
NlaIV GGNNCC 6 cut(s) 190, 342, 616, 1223, 1921, 2334
NmuCI GTSAC 4 cut(s) 272, 523, 1836, 2168
NsiI ATGCAT 1 cut(s) 1554
NspI RCATGY 6 cut(s) 838, 878, 992, 1458, 1552, 2153
NspV TTCGAA 1 cut(s) 264
PaeI GCATGC 2 cut(s) 838, 1458
PagI TCATGA 2 cut(s) 534, 1285
PctI GAATGC 3 cut(s) 308, 855, 942
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 9 cut(s) 152, 462, 766, 1167, 1343, 2125, 2174, 2180, 2507
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 2 cut(s) 933, 1853
PkrI GCNGC 8 cut(s) 920, 958, 1260, 1499, 1651, 1654, 1936, 2351
PleI GAGTC 2 cut(s) 2017, 2080
PpsI GAGTC 2 cut(s) 2017, 2080
PshBI ATTAAT 4 cut(s) 117, 443, 903, 1533
Psp1406I AACGTT 2 cut(s) 1249, 2466
Psp6I CCWGG 5 cut(s) 932, 946, 1123, 2119, 2353
PspEI GGTNACC 2 cut(s) 523, 777
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 5 cut(s) 932, 946, 1123, 2119, 2353
PspN4I GGNNCC 6 cut(s) 190, 342, 616, 1223, 1921, 2334
PstI CTGCAG 1 cut(s) 1654
PstNI CAGNNNCTG 2 cut(s) 680, 1853
PsuI RGATCY 3 cut(s) 340, 1880, 2187
PsyI GACNNNGTC 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 7 cut(s) 64, 418, 483, 610, 896, 995, 1995
RsaNI GTAC 7 cut(s) 63, 417, 482, 609, 895, 994, 1994
RseI CAYNNNNRTG 1 cut(s) 848
SatI GCNGC 8 cut(s) 919, 957, 1259, 1498, 1650, 1653, 1935, 2350
Sau3AI GATC 9 cut(s) 340, 733, 829, 883, 1880, 2135, 2158, 2187, 2324
ScaI AGTACT 2 cut(s) 418, 610
SchI GAGTC 2 cut(s) 2017, 2081
ScrFI CCNGG 6 cut(s) 819, 934, 948, 1125, 2121, 2355
SduI GDGCHC 1 cut(s) 1429
SexAI ACCWGGT 1 cut(s) 932
SfaNI GCATC 9 cut(s) 133, 932, 1260, 1302, 1398, 1561, 1876, 1946, 2306
SfcI CTRYAG 2 cut(s) 1650, 2454
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 2 cut(s) 412, 2486
SmoI CTYRAG 2 cut(s) 412, 2486
SpeI ACTAGT 1 cut(s) 2384
SphI GCATGC 2 cut(s) 838, 1458
SsiI CCGC 6 cut(s) 86, 540, 624, 918, 1935, 2319
SspI AATATT 1 cut(s) 1078
SspMI CTAG 8 cut(s) 278, 477, 803, 1242, 1703, 2109, 2385, 2514
StyD4I CCNGG 6 cut(s) 817, 932, 946, 1123, 2119, 2353
TaaI ACNGT 6 cut(s) 682, 899, 1187, 1352, 1489, 2523
TaiI ACGT 2 cut(s) 1252, 2469
TaqI TCGA 3 cut(s) 130, 264, 567
TaqII GACCGA 1 cut(s) 1824
TatI WGTACW 6 cut(s) 416, 481, 608, 894, 993, 1993
TauI GCSGC 2 cut(s) 921, 1937
TfiI GAWTC 9 cut(s) 152, 462, 766, 1167, 1343, 2125, 2174, 2180, 2507
TscAI CASTG 4 cut(s) 217, 736, 1465, 1914
TseFI GTSAC 4 cut(s) 272, 523, 1836, 2168
TseI GCWGC 6 cut(s) 956, 1258, 1497, 1649, 1652, 2349
Tsp45I GTSAC 4 cut(s) 272, 523, 1836, 2168
TspGWI ACGGA 3 cut(s) 548, 842, 2148
TspRI CASTG 4 cut(s) 217, 736, 1465, 1914
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 2 cut(s) 933, 1853
Vha464I CTTAAG 1 cut(s) 2486
VspI ATTAAT 4 cut(s) 117, 443, 903, 1533
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 6 cut(s) 838, 878, 992, 1458, 1552, 2153
XmiI GTMKAC 2 cut(s) 312, 1104
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 8 cut(s) 278, 477, 803, 1242, 1703, 2109, 2385, 2514
ZrmI AGTACT 2 cut(s) 418, 610
Zsp2I ATGCAT 1 cut(s) 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.