Prupe.5G028800_v2.0.a1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0007240
Physical Location & Seq
Reverse (-)
3389574 .. 3396412
6839 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G028800.1

Sequence Viewer

Length: 2682 bp
ATGGTGGAAGGAAGGGTTTGTTTGTCCAAAGAGGCCAAAAATGGATTAGAAATTCTGAAGCGTAGAAGGCTTCAGCGTATGAGATCGGAAACTGGTACCGAGCCTGTAAAGTTAACTAATATGATGGCTCGAAGCGGAGGGGATGCTTTAAAAGCTTCAGCTTCCTGTGGTATTAGATTACAGGGAGGTTCCGACCCAGTTTCTCTGTCCAGTGGTGCTTCGCATGGAAAAGATCTTTTTTCGAAGCGTAGGGTGGATAAGTTTGAAACAGGTGATCTAGACTGGACTGAGAAAATTCCTGAGTGTCCTGTATACTATCCAGCAAAGGAGGAGTTTGAGGATCCTTTGGTTTATTTACAGAAGATAGCTCCGGAAGCTTCAAAATATGGTATATGCAAGATTATATCCCCTTTGAGTGCTTCTACTCCAGCTGGGGTTGTATTGATGAGGGAGAAAGCAGGTTTCAAGTTCACAACTAGAGTACAGCCTCTTCGTCTTGCTGAGTGGGACAATGATGACAAGGTCACATTTTTCATGAGTGGAAGAAATTATACATTCCGTGATTTCGAGAAAATGGCGAACAAAGTTTTTGCTCGTCGATATTGTAGTTCTGGTTCTCTTCCTGCCACGTACATGGAAAAAGAATTTTGGCAAGAAATTGCTTGTGGAAAGACAGAAACTGTTGAATATGCATGTGATGTAGATGGCAGTGCCTTTTCGTCTTCTCGCAGTGATCCGCTTGGAAGTAGCAAATGGAATTTAAAGAATCTTTCAAGGTTACCCAATTCCATTTTGCGTCTTCTGGAAACTGCAATTCCGGGCGTAACTGATCCCATGCTATACATTGGAATGATATTTAGTATGTTTGCTTGGCACGTGGAGGATCATTATTTGTACAGTATTAATTATCATCATTGTGGTGCATCCAAAACCTGGTATGGAATTCCTGGTCAAGCTGCTCTGCAATTTGAAAAGGTAGTCAAGGAGCATGTGTACACGCATGATATCATATCAACCGATGGAGAGGATGGAGCTTTTGATGTATTATTGGGGAAAACAACTTTGTTTCCTCCAAATATTTTGTTGGAACACGATGTCCCTGTCTACAAGGCTGTGCAAAAGCCAGGAGAGTTTGTCGTTACTTTCCCTAGAGCATACCATGCTGGATTCAGTCATGGTTTCAACTGTGGTGAGGCAGTCAACTTTGCAATTGGTGATTGGTTCCCTTTAGGTGCCATTGCTAGCCGTCGTTATGCGCTTCTTAACAGGATGCCCCTGCTTCCTCACGAAGAACTTCTGTGTAAAGAAGCCATGCTTCTGTACACAAGTTTAGAACTTGAAGATTCTGAATATTCATCTGCTGATTTGGTCTCCCATCAATGCATTAAGACTTCTTTTGTTAGATTGATGCGCTTTCAGCATCGTGCTCGTTGGTCTTTAATGAAGTCAGGCGCATGCACTGGTGTTTTACCAAACTCCTACGGAACCGTTCTCTGCAGCCTGTGCAAGCGCGATTGCTATGTAGCTTACATTAATTGCAACTGTTACATGCATCCTGTGTGCCTTCGACATGAGGCAAAGTCTCTTGACTTCTCATGTGGAAGCAACCCTATTCTCTTTTTAAGAGAGGAGATAACAGAACTGGAAGCTGCAGCCAGAAAGTTTGAAATGGAAGATGGTATGTTAGAGGAGATAAAAGGGCTTGGTGAAAATGGCGATGACTATTATTCGTATCCGTTAATCTCGTTTCAGAGCACTGAAGAGAAAGGATACTCTCCTTATTGTGAGATAAAATTTGAGTTGAATCCTAAACTTACTGGCACAACTCATTATCGGTCACAAGAGCCAGAACCTGGTTCTCATGGTCAGCCCATGTTGAGTTGCGGTGCAAAATGTTCAAGTCCTGCAGTGTCAGATGGTTCCCTTTCATGTGCTGCATCAACTCTTTGTTCTCTTTTAGAGCCTCGTGAGAGCTTATCAGCACCCAATAATTGTTCATATGTTTCCTGGCAGGTACAGGGAAATGCTAACACTAACACAGGAGCTCTAAATTCTAAAAGGCTTTCTGAAGAATTAGCGCGCAGTACGTATGAGTCTTCTCAGTCCTCTCCATCATACAATGAATGTTCTAGTGCGCGCCCAAGAAATTGTAATGGATCAGAGGTTAGGCCTGTTGTGGATCAAGGCAGTGATGATTCTGATTCTGAGATCTTTAGGGTTAAGCGCCGCTCATCATTGAAGGTGGACAAAAGAAGTGTAAATGATATCTCGTCATCAAAGCATTCTGAGAACCAGGGTTTTAAGCGACTGAAGAAACTGCAAGCTGAAAGATGTGGGCCATCGGTCCCACAGTATTCTTGCAGCACTGGTGAATCAACTAGGTTTCTTACAACTAGTACTTACAAAGGAGTTTCTGAGAGTGCTCCTATGGAGGGCAGGTTTTCCAGAGGAAGTACCGTCGTCCCTAGAGGAAGTACAGTTCCCATCTCTATCAAGTTTAAGAAGTTGGCCAATGAAGAATCGGTTAGTAGACAGCGAGAACACCACAGAAAGGATAGATACCACCAGCTTGAGTCGGGAAAGAGACGGAGGGGACCACCGCCAATAGAGATTGGTCCGAAGCACCTTAAAGTCAAAGGCCCGTCATTCCTAGGGTCAGAGAGCAGCAGCAGGTTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0000792 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010016 GO:0010033 GO:0010228 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032454 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045815 GO:0048366 GO:0048367 GO:0048439 GO:0048518 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

894

Amino Acids

99.6

Weight (kDa)

8.27

Isoelectric Point (pI)

54.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 449, 2002, 2427, 2661
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 95, 1232
AccB7I CCANNNNNTGG 2 cut(s) 933, 1853
AccBSI CCGCTC 1 cut(s) 2229
AccI GTMKAC 3 cut(s) 312, 1104, 2530
AccII CGCG 3 cut(s) 1512, 2080, 2137
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 5 cut(s) 135, 737, 1884, 2227, 2600
AclWI GGATC 7 cut(s) 335, 348, 728, 824, 891, 2164, 2187
AcoI YGGCCR 1 cut(s) 2508
AcsI RAATTY 7 cut(s) 51, 294, 644, 757, 942, 1793, 2050
AcuI CTGAAG 6 cut(s) 56, 77, 141, 1779, 2088, 2330
AcvI CACGTG 1 cut(s) 877
AfiI CCNNNNNNNGG 4 cut(s) 933, 1853, 2432, 2551
AhlI ACTAGT 1 cut(s) 2393
AjnI CCWGG 6 cut(s) 932, 946, 1123, 1852, 2006, 2292
AloI GAACNNNNNNTCC 4 cut(s) 1080, 1112, 2463, 2495
Alw21I GWGCWC 4 cut(s) 1429, 1757, 2047, 2425
Alw26I GTCTC 3 cut(s) 1375, 1587, 2578
AlwI GGATC 7 cut(s) 335, 348, 728, 824, 891, 2164, 2187
AlwNI CAGNNNCTG 2 cut(s) 680, 1853
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 33, 2168, 2336, 2508, 2638
ApeKI GCWGC 8 cut(s) 956, 1497, 1649, 1652, 1934, 2361, 2664, 2667
ApoI RAATTY 7 cut(s) 51, 294, 644, 757, 942, 1793, 2050
AseI ATTAAT 2 cut(s) 903, 1533
Asp700I GAANNNNTTC 1 cut(s) 1293
Asp718I GGTACC 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 2650
AspLEI GCGC 9 cut(s) 1258, 1413, 1454, 1512, 2080, 2082, 2137, 2139, 2226
AspS9I GGNCC 5 cut(s) 2336, 2344, 2594, 2615, 2639
AsuC2I CCSGG 1 cut(s) 819
AsuHPI GGTGA 5 cut(s) 284, 1202, 1226, 1718, 2381
AsuII TTCGAA 1 cut(s) 242
AsuNHI GCTAGC 1 cut(s) 1241
AvaII GGWCC 3 cut(s) 2344, 2594, 2615
AvrII CCTAGG 1 cut(s) 2650
BalI TGGCCA 1 cut(s) 2510
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 95, 1232
BanII GRGCYC 1 cut(s) 2047
BarI GAAGNNNNNNTAC 4 cut(s) 474, 506, 535, 567
BauI CACGAG 1 cut(s) 1965
BbrPI CACGTG 1 cut(s) 877
BbsI GAAGAC 3 cut(s) 714, 791, 2088
Bbv12I GWGCWC 4 cut(s) 1429, 1757, 2047, 2425
BbvI GCAGC 7 cut(s) 943, 1509, 1636, 1664, 1921, 2373, 2676
BceAI ACGGC 1 cut(s) 1230
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 6 cut(s) 934, 948, 1125, 1854, 2008, 2294
BciVI GTATCC 2 cut(s) 1743, 1763
BcnI CCSGG 1 cut(s) 819
BcoDI GTCTC 3 cut(s) 1375, 1587, 2578
BcuI ACTAGT 1 cut(s) 2393
BfaI CTAG 9 cut(s) 278, 477, 1149, 1242, 2130, 2379, 2394, 2466, 2651
BfmI CTRYAG 3 cut(s) 1495, 1650, 1905
BfoI RGCGCY 1 cut(s) 2227
BfuAI ACCTGC 4 cut(s) 449, 2002, 2427, 2661
BfuI GTATCC 2 cut(s) 1743, 1763
BglII AGATCT 2 cut(s) 232, 2208
BisI GCNGC 9 cut(s) 957, 1498, 1650, 1653, 1935, 2227, 2362, 2665, 2668
BlnI CCTAGG 1 cut(s) 2650
BlsI GCNGC 9 cut(s) 958, 1499, 1651, 1654, 1936, 2228, 2363, 2666, 2669
BmcAI AGTACT 1 cut(s) 2398
Bme1390I CCNGG 7 cut(s) 819, 934, 948, 1125, 1854, 2008, 2294
Bme18I GGWCC 3 cut(s) 2344, 2594, 2615
BmgT120I GGNCC 5 cut(s) 2336, 2344, 2594, 2615, 2639
BmiI GGNNCC 9 cut(s) 97, 190, 342, 1223, 1234, 1486, 1921, 2346, 2595
BmrFI CCNGG 7 cut(s) 819, 934, 948, 1125, 1854, 2008, 2294
BmrI ACTGGG 1 cut(s) 191
BmsI GCATC 7 cut(s) 133, 932, 1260, 1398, 1429, 1561, 1946
BmtI GCTAGC 1 cut(s) 1245
BmuI ACTGGG 1 cut(s) 191
BpiI GAAGAC 3 cut(s) 714, 791, 2088
BpmI CTGGAG 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 2591
BpuMI CCSGG 1 cut(s) 819
BsaAI YACGTR 3 cut(s) 630, 877, 2088
BsaI GGTCTC 1 cut(s) 1375
BsaJI CCNNGG 2 cut(s) 2293, 2650
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 6 cut(s) 977, 1007, 1119, 1149, 2423, 2453
Bsc4I CCNNNNNNNGG 4 cut(s) 933, 1853, 2432, 2551
Bse1I ACTGG 8 cut(s) 97, 197, 210, 287, 1465, 1647, 1822, 2371
Bse3DI GCAATG 1 cut(s) 1236
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 6 cut(s) 934, 948, 1125, 1854, 2008, 2294
BseDI CCNNGG 2 cut(s) 2293, 2650
BseGI GGATG 5 cut(s) 148, 923, 1033, 1275, 1552
BseLI CCNNNNNNNGG 4 cut(s) 933, 1853, 2432, 2551
BseMI GCAATG 1 cut(s) 1236
BseMII CTCAG 7 cut(s) 279, 291, 492, 2114, 2196, 2277, 2406
BseNI ACTGG 8 cut(s) 97, 197, 210, 287, 1465, 1647, 1822, 2371
BsePI GCGCGC 2 cut(s) 2078, 2135
BseRI GAGGAG 3 cut(s) 344, 1643, 1703
BseXI GCAGC 7 cut(s) 943, 1509, 1636, 1664, 1921, 2373, 2676
BseYI CCCAGC 1 cut(s) 431
Bsh1236I CGCG 3 cut(s) 1512, 2080, 2137
BshFI GGCC 5 cut(s) 35, 2170, 2338, 2510, 2640
BshNI GGYRCC 2 cut(s) 95, 1232
BsiHKAI GWGCWC 4 cut(s) 1429, 1757, 2047, 2425
BsiSI CCGG 2 cut(s) 371, 818
BslFI GGGAC 5 cut(s) 521, 1082, 2330, 2447, 2607
BslI CCNNNNNNNGG 4 cut(s) 933, 1853, 2432, 2551
BsmAI GTCTC 3 cut(s) 1375, 1587, 2578
BsmBI CGTCTC 1 cut(s) 2578
BsmFI GGGAC 5 cut(s) 521, 1082, 2330, 2447, 2607
BsmI GAATGC 1 cut(s) 2281
BsnI GGCC 5 cut(s) 35, 2170, 2338, 2510, 2640
Bso31I GGTCTC 1 cut(s) 1375
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 1429, 1757, 2047, 2425
Bsp13I TCCGGA 1 cut(s) 370
Bsp1407I TGTACA 3 cut(s) 894, 993, 1320
BspACI CCGC 5 cut(s) 135, 737, 1884, 2227, 2600
BspANI GGCC 5 cut(s) 35, 2170, 2338, 2510, 2640
BspCNI CTCAG 7 cut(s) 280, 292, 493, 2113, 2197, 2278, 2407
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 3 cut(s) 1512, 2080, 2137
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 9 cut(s) 97, 190, 342, 1223, 1234, 1486, 1921, 2346, 2595
BspMAI CTGCAG 3 cut(s) 1499, 1654, 1909
BspMI ACCTGC 4 cut(s) 449, 2002, 2427, 2661
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 7 cut(s) 335, 348, 728, 824, 891, 2164, 2187
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 2 cut(s) 95, 1232
BspTNI GGTCTC 1 cut(s) 1375
BsrBI CCGCTC 1 cut(s) 2229
BsrDI GCAATG 1 cut(s) 1236
BsrGI TGTACA 3 cut(s) 894, 993, 1320
BsrI ACTGG 8 cut(s) 97, 197, 210, 287, 1465, 1647, 1822, 2371
BssECI CCNNGG 2 cut(s) 2293, 2650
BssHII GCGCGC 2 cut(s) 2078, 2135
BssNAI GTATAC 1 cut(s) 313
BssSI CACGAG 1 cut(s) 1965
BssT1I CCWWGG 1 cut(s) 2650
Bst1107I GTATAC 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 1965
Bst2UI CCWGG 6 cut(s) 934, 948, 1125, 1854, 2008, 2294
Bst4CI ACNGT 8 cut(s) 682, 899, 1187, 1489, 1544, 2352, 2458, 2479
Bst6I CTCTTC 3 cut(s) 495, 624, 1755
BstAPI GCANNNNNTGC 2 cut(s) 1160, 1503
BstAUI TGTACA 3 cut(s) 894, 993, 1320
BstBAI YACGTR 3 cut(s) 630, 877, 2088
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 6 cut(s) 1243, 1456, 1508, 2080, 2137, 2322
BstDEI CTNAG 7 cut(s) 288, 300, 501, 2100, 2205, 2286, 2415
BstEII GGTNACC 1 cut(s) 777
BstF5I GGATG 5 cut(s) 148, 923, 1033, 1275, 1552
BstFNI CGCG 3 cut(s) 1512, 2080, 2137
BstH2I RGCGCY 1 cut(s) 2227
BstHHI GCGC 9 cut(s) 1258, 1413, 1454, 1512, 2080, 2082, 2137, 2139, 2226
BstMAI GTCTC 3 cut(s) 1375, 1587, 2578
BstMWI GCNNNNNNNGC 6 cut(s) 67, 152, 374, 1160, 1417, 1503
BstNI CCWGG 6 cut(s) 934, 948, 1125, 1854, 2008, 2294
BstNSI RCATGY 4 cut(s) 696, 992, 1458, 1552
BstPI GGTNACC 1 cut(s) 777
BstSCI CCNGG 7 cut(s) 817, 932, 946, 1123, 1852, 2006, 2292
BstSFI CTRYAG 3 cut(s) 1495, 1650, 1905
BstSNI TACGTA 1 cut(s) 2088
BstUI CGCG 3 cut(s) 1512, 2080, 2137
BstV1I GCAGC 7 cut(s) 943, 1509, 1636, 1664, 1921, 2373, 2676
BstV2I GAAGAC 3 cut(s) 714, 791, 2088
BstX2I RGATCY 3 cut(s) 232, 340, 2208
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 3 cut(s) 232, 340, 2208
BstZ17I GTATAC 1 cut(s) 313
BsuI GTATCC 2 cut(s) 1743, 1763
BsuRI GGCC 5 cut(s) 35, 2170, 2338, 2510, 2640
BtgZI GCGATG 1 cut(s) 1731
BtsCI GGATG 5 cut(s) 148, 923, 1033, 1275, 1552
BtsI GCAGTG 4 cut(s) 715, 736, 1914, 2194
BtsIMutI CAGTG 8 cut(s) 217, 715, 736, 1458, 1755, 1914, 2194, 2364
BveI ACCTGC 4 cut(s) 449, 2002, 2427, 2661
Cac8I GCNNGC 6 cut(s) 1243, 1456, 1508, 2080, 2137, 2322
CaiI CAGNNNCTG 2 cut(s) 680, 1853
CciI TCATGA 1 cut(s) 534
CfoI GCGC 9 cut(s) 1258, 1413, 1454, 1512, 2080, 2082, 2137, 2139, 2226
Cfr13I GGNCC 5 cut(s) 2336, 2344, 2594, 2615, 2639
CseI GACGC 1 cut(s) 785
CsiI ACCWGGT 2 cut(s) 932, 1852
DdeI CTNAG 7 cut(s) 288, 300, 501, 2100, 2205, 2286, 2415
DraI TTTAAA 2 cut(s) 150, 762
EaeI YGGCCR 1 cut(s) 2508
Eam1104I CTCTTC 3 cut(s) 495, 624, 1755
EarI CTCTTC 3 cut(s) 495, 624, 1755
Ecl136II GAGCTC 1 cut(s) 2045
Eco105I TACGTA 1 cut(s) 2088
Eco130I CCWWGG 1 cut(s) 2650
Eco147I AGGCCT 1 cut(s) 2170
Eco24I GRGCYC 1 cut(s) 2047
Eco31I GGTCTC 1 cut(s) 1375
Eco32I GATATC 2 cut(s) 1006, 2266
Eco47I GGWCC 3 cut(s) 2344, 2594, 2615
Eco53kI GAGCTC 1 cut(s) 2045
Eco57I CTGAAG 6 cut(s) 56, 77, 141, 1779, 2088, 2330
Eco72I CACGTG 1 cut(s) 877
Eco91I GGTNACC 1 cut(s) 777
EcoICRI GAGCTC 1 cut(s) 2045
EcoO65I GGTNACC 1 cut(s) 777
EcoRI GAATTC 1 cut(s) 942
EcoRII CCWGG 6 cut(s) 932, 946, 1123, 1852, 2006, 2292
EcoRV GATATC 2 cut(s) 1006, 2266
EcoT14I CCWWGG 1 cut(s) 2650
EcoT22I ATGCAT 3 cut(s) 694, 1385, 1554
EcoT38I GRGCYC 1 cut(s) 2047
ErhI CCWWGG 1 cut(s) 2650
Esp3I CGTCTC 1 cut(s) 2578
FalI AAGNNNNNCTT 2 cut(s) 1299, 1331
FaqI GGGAC 5 cut(s) 521, 1082, 2330, 2447, 2607
FauNDI CATATG 1 cut(s) 1999
FblI GTMKAC 3 cut(s) 312, 1104, 2530
Fnu4HI GCNGC 9 cut(s) 957, 1498, 1650, 1653, 1935, 2227, 2362, 2665, 2668
FokI GGATG 5 cut(s) 155, 910, 1040, 1282, 1539
FriOI GRGCYC 1 cut(s) 2047
Fsp4HI GCNGC 9 cut(s) 957, 1498, 1650, 1653, 1935, 2227, 2362, 2665, 2668
FspBI CTAG 9 cut(s) 278, 477, 1149, 1242, 2130, 2379, 2394, 2466, 2651
GlaI GCGC 9 cut(s) 1257, 1412, 1453, 1511, 2079, 2081, 2136, 2138, 2225
GluI GCNGC 9 cut(s) 957, 1498, 1650, 1653, 1935, 2227, 2362, 2665, 2668
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 2227
HaeIII GGCC 5 cut(s) 35, 2170, 2338, 2510, 2640
HapII CCGG 2 cut(s) 371, 818
HgaI GACGC 1 cut(s) 785
HhaI GCGC 9 cut(s) 1258, 1413, 1454, 1512, 2080, 2082, 2137, 2139, 2226
Hin6I GCGC 9 cut(s) 1256, 1411, 1452, 1510, 2078, 2080, 2135, 2137, 2224
HinP1I GCGC 9 cut(s) 1256, 1411, 1452, 1510, 2078, 2080, 2135, 2137, 2224
HincII GTYRAC 2 cut(s) 114, 1201
HindII GTYRAC 2 cut(s) 114, 1201
HindIII AAGCTT 2 cut(s) 153, 375
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 2 cut(s) 371, 818
HphI GGTGA 5 cut(s) 284, 1202, 1226, 1718, 2381
Hpy99I CGWCG 3 cut(s) 600, 1251, 2462
HpyAV CCTTC 4 cut(s) 6, 60, 1574, 2233
HpyCH4III ACNGT 8 cut(s) 682, 899, 1187, 1489, 1544, 2352, 2458, 2479
HpyCH4IV ACGT 3 cut(s) 629, 876, 2087
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 152, 374, 1160, 1417, 1503
HpyF3I CTNAG 7 cut(s) 288, 300, 501, 2100, 2205, 2286, 2415
HpySE526I ACGT 3 cut(s) 629, 876, 2087
HspAI GCGC 9 cut(s) 1256, 1411, 1452, 1510, 2078, 2080, 2135, 2137, 2224
Kpn2I TCCGGA 1 cut(s) 370
KpnI GGTACC 1 cut(s) 99
KspAI GTTAAC 1 cut(s) 114
LmnI GCTCC 5 cut(s) 373, 985, 1031, 2042, 2428
Lsp1109I GCAGC 7 cut(s) 943, 1509, 1636, 1664, 1921, 2373, 2676
LweI GCATC 7 cut(s) 133, 932, 1260, 1398, 1429, 1561, 1946
MabI ACCWGGT 2 cut(s) 932, 1852
MaeI CTAG 9 cut(s) 278, 477, 1149, 1242, 2130, 2379, 2394, 2466, 2651
MaeII ACGT 3 cut(s) 629, 876, 2087
MaeIII GTNAC 6 cut(s) 523, 777, 823, 1138, 1544, 1836
MbiI CCGCTC 1 cut(s) 2229
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 3 cut(s) 232, 340, 2208
MhlI GDGCHC 4 cut(s) 1429, 1757, 2047, 2425
MlsI TGGCCA 1 cut(s) 2510
MluNI TGGCCA 1 cut(s) 2510
MlyI GAGTC 2 cut(s) 2102, 2582
MmeI TCCRAC 2 cut(s) 216, 1065
Mox20I TGGCCA 1 cut(s) 2510
Mph1103I ATGCAT 3 cut(s) 694, 1385, 1554
MroI TCCGGA 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 1 cut(s) 2510
MslI CAYNNNNRTG 4 cut(s) 632, 848, 915, 918
Msp20I TGGCCA 1 cut(s) 2510
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 2 cut(s) 371, 818
MspR9I CCNGG 7 cut(s) 819, 934, 948, 1125, 1854, 2008, 2294
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 1 cut(s) 2281
MvaI CCWGG 6 cut(s) 934, 948, 1125, 1854, 2008, 2294
MvnI CGCG 3 cut(s) 1512, 2080, 2137
MwoI GCNNNNNNNGC 6 cut(s) 67, 152, 374, 1160, 1417, 1503
NciI CCSGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 1999
NheI GCTAGC 1 cut(s) 1241
NlaIV GGNNCC 9 cut(s) 97, 190, 342, 1223, 1234, 1486, 1921, 2346, 2595
NmuCI GTSAC 2 cut(s) 523, 1836
NsiI ATGCAT 3 cut(s) 694, 1385, 1554
NspI RCATGY 4 cut(s) 696, 992, 1458, 1552
NspV TTCGAA 1 cut(s) 242
PaeI GCATGC 1 cut(s) 1458
PagI TCATGA 1 cut(s) 534
PauI GCGCGC 2 cut(s) 2078, 2135
PceI AGGCCT 1 cut(s) 2170
PctI GAATGC 1 cut(s) 2281
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 8 cut(s) 766, 1167, 1343, 1804, 2195, 2201, 2372, 2519
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 2 cut(s) 933, 1853
PkrI GCNGC 9 cut(s) 958, 1499, 1651, 1654, 1936, 2228, 2363, 2666, 2669
PleI GAGTC 2 cut(s) 2101, 2581
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 2 cut(s) 2101, 2581
Ppu21I YACGTR 3 cut(s) 630, 877, 2088
PshBI ATTAAT 2 cut(s) 903, 1533
Psp124BI GAGCTC 1 cut(s) 2047
Psp6I CCWGG 6 cut(s) 932, 946, 1123, 1852, 2006, 2292
PspCI CACGTG 1 cut(s) 877
PspEI GGTNACC 1 cut(s) 777
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 6 cut(s) 932, 946, 1123, 1852, 2006, 2292
PspN4I GGNNCC 9 cut(s) 97, 190, 342, 1223, 1234, 1486, 1921, 2346, 2595
PspPI GGNCC 5 cut(s) 2336, 2344, 2594, 2615, 2639
PsrI GAACNNNNNNTAC 2 cut(s) 598, 630
PstI CTGCAG 3 cut(s) 1499, 1654, 1909
PstNI CAGNNNCTG 2 cut(s) 680, 1853
PsuI RGATCY 3 cut(s) 232, 340, 2208
PsyI GACNNNGTC 1 cut(s) 521
PteI GCGCGC 2 cut(s) 2078, 2135
PvuII CAGCTG 1 cut(s) 431
RseI CAYNNNNRTG 4 cut(s) 632, 848, 915, 918
SacI GAGCTC 1 cut(s) 2047
SatI GCNGC 9 cut(s) 957, 1498, 1650, 1653, 1935, 2227, 2362, 2665, 2668
Sau96I GGNCC 5 cut(s) 2336, 2344, 2594, 2615, 2639
ScaI AGTACT 1 cut(s) 2398
SchI GAGTC 2 cut(s) 2102, 2582
ScrFI CCNGG 7 cut(s) 819, 934, 948, 1125, 1854, 2008, 2294
SduI GDGCHC 4 cut(s) 1429, 1757, 2047, 2425
SexAI ACCWGGT 2 cut(s) 932, 1852
SfaNI GCATC 7 cut(s) 133, 932, 1260, 1398, 1429, 1561, 1946
SfcI CTRYAG 3 cut(s) 1495, 1650, 1905
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 3 cut(s) 2344, 2594, 2615
SmiMI CAYNNNNRTG 4 cut(s) 632, 848, 915, 918
SmlI CTYRAG 1 cut(s) 2570
SmoI CTYRAG 1 cut(s) 2570
SnaBI TACGTA 1 cut(s) 2088
SpeI ACTAGT 1 cut(s) 2393
SphI GCATGC 1 cut(s) 1458
SseBI AGGCCT 1 cut(s) 2170
SsiI CCGC 5 cut(s) 135, 737, 1884, 2227, 2600
SspI AATATT 2 cut(s) 1078, 1352
SspMI CTAG 9 cut(s) 278, 477, 1149, 1242, 2130, 2379, 2394, 2466, 2651
SstI GAGCTC 1 cut(s) 2047
StuI AGGCCT 1 cut(s) 2170
StyD4I CCNGG 7 cut(s) 817, 932, 946, 1123, 1852, 2006, 2292
StyI CCWWGG 1 cut(s) 2650
TaaI ACNGT 8 cut(s) 682, 899, 1187, 1489, 1544, 2352, 2458, 2479
TaiI ACGT 3 cut(s) 632, 879, 2090
TaqI TCGA 5 cut(s) 130, 242, 567, 598, 1567
TaqII GACCGA 2 cut(s) 1824, 2332
TatI WGTACW 6 cut(s) 481, 894, 993, 1320, 2396, 2474
TauI GCSGC 1 cut(s) 2229
TfiI GAWTC 8 cut(s) 766, 1167, 1343, 1804, 2195, 2201, 2372, 2519
TscAI CASTG 8 cut(s) 217, 715, 736, 1465, 1762, 1914, 2194, 2371
TseFI GTSAC 2 cut(s) 523, 1836
TseI GCWGC 8 cut(s) 956, 1497, 1649, 1652, 1934, 2361, 2664, 2667
Tsp45I GTSAC 2 cut(s) 523, 1836
TspDTI ATGAA 7 cut(s) 523, 1344, 1457, 1917, 1986, 2136, 2529
TspGWI ACGGA 4 cut(s) 548, 1497, 1725, 2602
TspRI CASTG 8 cut(s) 217, 715, 736, 1465, 1762, 1914, 2194, 2371
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 2 cut(s) 933, 1853
VpaK11BI GGWCC 3 cut(s) 2344, 2594, 2615
VspI ATTAAT 2 cut(s) 903, 1533
XapI RAATTY 7 cut(s) 51, 294, 644, 757, 942, 1793, 2050
XbaI TCTAGA 1 cut(s) 277
XceI RCATGY 4 cut(s) 696, 992, 1458, 1552
XmaJI CCTAGG 1 cut(s) 2650
XmiI GTMKAC 3 cut(s) 312, 1104, 2530
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 9 cut(s) 278, 477, 1149, 1242, 2130, 2379, 2394, 2466, 2651
ZrmI AGTACT 1 cut(s) 2398
Zsp2I ATGCAT 3 cut(s) 694, 1385, 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.