Rmu_sc0001875.1_g000012

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001875.1
OG0007240
Physical Location & Seq
Forward (+)
45852 .. 52153
6302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001875.1_g000012.1.cds

Sequence Viewer

Length: 2856 bp
ctgtattcgccggaaaacctccttttcctcgccggaatatctcctttcgccggcgaataccgtccatctgtagccgggaaaccgcaacgcctccgtcgcctgaagactctcttcaactggagagcctctttcgttgactccgtcgttgaaggccttggatctccgagatacacaccattcgctgccgttggggacgttttgcgagaatgtgggctgccgtggtctcgctcaaggcttttgcggcgtctccggaatgagatggtggaaggtagggtttgtttttccaaagaggcccaaaatggattagaatttctgaagcgaagaaggcttcagcatatgagatcagaaactattcctgagcctgtaagtttcacgaatatgatgtctagaagtggaggggatgctttaaaagcttcagcatcctgtggtgttagaatacatggaggtgccgattcaatttctcggtccaatggtgctccacaggggaaagatgttttttcaaagcgtaaggtggataaatttgaaacaagtgatctagactggaccgaaaaaattcccgagtgtcctgtatactatccagcaaaggaggagtttgaggatcctttagtctatttacagaaaatagctccagaagcttccaaatatggtatatgcaagattatctcccctgttagtgcttctactcctgctggggttgtattgatgagggaaaaagcagggtttaagttcactactagagtacaacctcttcgccttgccgagtgggacaatgatgacagggtcacttttttcatgagcggaagaaactatacattccgtgatttcgagaaaatggcgaacaaggttttcgctcgtagatactgtagtgctgggtctcttcctcctacgtacttggaaaaagaattttggaaagaaattggttctggaaagacagaaagtgttgaatatgcctgtgatgtagatggtagtgcattttcatgttctcccaatgatccacttggaagtagtaaatggaatttaaagaatctttcaaggctacccaagtctattctgcgtcttctggaaaacgcaattccgggtgtaacagatcctatgctatacattggaatgatatttagcatgtttgcttggcatgtggaagatcactatttgtacagtatcaattatcatcattgtggcgcatccaaaacctggtatggcattccaggtcatgccgccctgcaatttgaaaaggttgtcaaggaacatgtttacaccaatgatattgtaccaagtgacggagaggatggagcttttgatgtcttattggggaaaacaactctgtttcctcccagcatattgttggaacatgatgtacctgtctacaaagctgtgcaaaagccaggagagtttgtggttactttccctagagcgtatcatgctggattcagtcatggtttcaattgtggtgaggcagtcaactttgctattggtgattggttcccattgggtgctattgctagccggcgttatgcacttcttaataggatgcctctgctccctcatgaagaactcctgtgcaaagaagccatgcttctccatacaagtttagagcttgaagattcagattattcttctgctgacttggtttcccatcatcgcattaaggtctcttttgtgaaactgatgcggtttcagcatcgtgctcgttggactttaatgaagtctggggcatgcactggtgtcttgccaaactcatatggaactgttctctgcagcatatgcaagcgcgattgctatcttgcttacattagttgcaactgttatatgcatcatgtgtgccttcgccatgatataaggtcattggacttgtcatgtcagagcaatcctactctctttttaagggaggagatagcagaaatggaagctgcagcaagtaaatttgaagaggaagcggaagtgatagaggagattcaaggtcaaggtgaaaatggtgatgacttgtattcatatccattaattttgtctccgatagctgaagagaaagggtactccccttactgcaatataaaatttgagttgaaccccgaactccctggtacaacagaaatcactggcacaactctggctcagtcacaagagccagctgcacttggttctcataccagtcctgcagtgtcagaaggttccatttcatgtattgcatctactctttgttctctttcagagccacttgagagcttgtatgcacccaataatggaattgccaactctaagacagtgactctaaattccaaaaggtctgcatatgaggcttctcggtcctccccatcatatgatgaatgctcaagtgtatgtccaggaagttctaacgcattggaggttaggcctatagtagatcaaggcagtgatgattctgattcagagatatttagggttaagcgccgttcttcattgaaggtggagagaagaattataaatgatgtcttgccatcaaatcattctgagaaccagggatttaagcgactgaagaaactgcaacctgatcctagatgtgggcggtcggtgccattacaaagctgcagcactaccgaatcaagtactaagttggttacaactactaatagcaaaggggctccggagaatgctttaagagatgacaggtttgccagaggaagtaccattcccttctctatcaagtttaagaagttgaccaatgaagattctgttaggagacagcgagatcaccacagaactgatatacgccagcttgagttgggaaagagccgcagggaacctcccccaatagagattgggccaaagcgccttaaggtcaaaggcccttctttcttagcttcggagaacaggttaaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0000792 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010016 GO:0010033 GO:0010228 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032454 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045815 GO:0048366 GO:0048367 GO:0048439 GO:0048518 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

951

Amino Acids

106.32

Weight (kDa)

8.14

Isoelectric Point (pI)

57.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2456
AccB1I GGYRCC 2 cut(s) 446, 2545
AccB7I CCANNNNNTGG 1 cut(s) 1191
AccBSI CCGCTC 1 cut(s) 798
AccI GTMKAC 2 cut(s) 570, 1362
AccII CGCG 1 cut(s) 1770
AccIII TCCGGA 2 cut(s) 249, 2617
AciI CCGC 8 cut(s) 83, 241, 798, 1215, 1669, 1934, 2539, 2767
AclWI GGATC 6 cut(s) 166, 593, 606, 986, 1082, 2519
AcsI RAATTY 8 cut(s) 308, 518, 552, 902, 1015, 1919, 2051, 2269
AcuI CTGAAG 6 cut(s) 122, 314, 335, 399, 2037, 2528
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 9 cut(s) 741, 890, 1154, 1269, 1356, 2030, 2080, 2581, 2659
AfiI CCNNNNNNNGG 5 cut(s) 50, 1191, 1277, 2237, 2534
AflII CTTAAG 1 cut(s) 2807
AflIII ACRYGT 1 cut(s) 1246
AjnI CCWGG 6 cut(s) 1190, 1204, 1381, 2074, 2338, 2490
Alw21I GWGCWC 2 cut(s) 478, 1687
Alw26I GTCTC 6 cut(s) 228, 251, 879, 1654, 2010, 2707
AlwI GGATC 6 cut(s) 166, 593, 606, 986, 1082, 2519
Ama87I CYCGRG 1 cut(s) 557
Aor13HI TCCGGA 2 cut(s) 249, 2617
AoxI GGCC 5 cut(s) 151, 291, 2366, 2795, 2818
ApeKI GCWGC 8 cut(s) 182, 214, 1755, 1907, 1910, 2126, 2559, 2562
ApoI RAATTY 8 cut(s) 308, 518, 552, 902, 1015, 1919, 2051, 2269
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AseI ATTAAT 1 cut(s) 1997
Asp700I GAANNNNTTC 2 cut(s) 351, 552
AspLEI GCGC 4 cut(s) 1181, 1770, 2424, 2805
AspS9I GGNCC 6 cut(s) 292, 465, 543, 2301, 2795, 2819
AsuC2I CCSGG 2 cut(s) 76, 1077
AsuHPI GGTGA 5 cut(s) 1460, 1484, 1976, 1985, 2717
AsuNHI GCTAGC 1 cut(s) 1499
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 3 cut(s) 465, 543, 2301
BaeI ACNNNNGTAYC 2 cut(s) 850, 883
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 2 cut(s) 446, 2545
BanII GRGCYC 1 cut(s) 2617
BarI GAAGNNNNNNTAC 4 cut(s) 732, 764, 793, 825
BbsI GAAGAC 2 cut(s) 110, 1049
Bbv12I GWGCWC 2 cut(s) 478, 1687
BbvI GCAGC 8 cut(s) 169, 201, 1767, 1894, 1922, 2113, 2546, 2574
BccI CCATC 7 cut(s) 73, 253, 956, 1280, 1641, 2317, 2479
BceAI ACGGC 3 cut(s) 170, 202, 2409
BcgI CGANNNNNNTGC 2 cut(s) 1667, 1701
BciT130I CCWGG 6 cut(s) 1192, 1206, 1383, 2076, 2340, 2492
BcnI CCSGG 2 cut(s) 76, 1077
BcoDI GTCTC 6 cut(s) 228, 251, 879, 1654, 2010, 2707
BfaI CTAG 6 cut(s) 387, 536, 735, 1407, 1500, 2529
BfmI CTRYAG 7 cut(s) 69, 862, 1753, 1908, 2151, 2370, 2560
BfoI RGCGCY 2 cut(s) 2425, 2806
BfrI CTTAAG 1 cut(s) 2807
BmcAI AGTACT 1 cut(s) 2581
Bme1390I CCNGG 8 cut(s) 76, 1077, 1192, 1206, 1383, 2076, 2340, 2492
Bme18I GGWCC 3 cut(s) 465, 543, 2301
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 6 cut(s) 292, 465, 543, 2301, 2795, 2819
BmiI GGNNCC 7 cut(s) 448, 600, 1481, 2167, 2547, 2616, 2775
BmrFI CCNGG 8 cut(s) 76, 1077, 1192, 1206, 1383, 2076, 2340, 2492
BmsI GCATC 8 cut(s) 391, 428, 1190, 1518, 1656, 1687, 1819, 2192
BmtI GCTAGC 1 cut(s) 1503
BpiI GAAGAC 2 cut(s) 110, 1049
BpmI CTGGAG 2 cut(s) 139, 612
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 4 cut(s) 214, 2234, 2311, 2771
BpuMI CCSGG 2 cut(s) 76, 1077
BsaAI YACGTR 1 cut(s) 888
BsaBI GATNNNNATC 1 cut(s) 1776
BsaHI GRCGYC 1 cut(s) 244
BsaI GGTCTC 3 cut(s) 228, 879, 1654
BsaJI CCNNGG 4 cut(s) 154, 218, 2074, 2491
BsaWI WCCGGW 2 cut(s) 249, 2617
BsaXI ACNNNNNCTCC 3 cut(s) 1377, 1407, 2831
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 1191, 1277, 2237, 2534
Bse118I RCCGGY 2 cut(s) 50, 1503
Bse1I ACTGG 5 cut(s) 122, 545, 1723, 2098, 2145
Bse8I GATNNNNATC 1 cut(s) 1776
BseAI TCCGGA 2 cut(s) 249, 2617
BseBI CCWGG 6 cut(s) 1192, 1206, 1383, 2076, 2340, 2492
BseDI CCNNGG 4 cut(s) 154, 218, 2074, 2491
BseGI GGATG 5 cut(s) 406, 419, 1181, 1291, 1533
BseJI GATNNNNATC 1 cut(s) 1776
BseLI CCNNNNNNNGG 5 cut(s) 50, 1191, 1277, 2237, 2534
BseMII CTCAG 3 cut(s) 348, 2123, 2475
BseNI ACTGG 5 cut(s) 122, 545, 1723, 2098, 2145
BseRI GAGGAG 3 cut(s) 602, 1901, 1961
BseXI GCAGC 8 cut(s) 169, 201, 1767, 1894, 1922, 2113, 2546, 2574
BseYI CCCAGC 3 cut(s) 689, 869, 1331
BsgI GTGCAG 1 cut(s) 2112
Bsh1236I CGCG 1 cut(s) 1770
Bsh1285I CGRYCG 1 cut(s) 2543
BshFI GGCC 5 cut(s) 153, 293, 2368, 2797, 2820
BshNI GGYRCC 2 cut(s) 446, 2545
BsiEI CGRYCG 1 cut(s) 2543
BsiHKAI GWGCWC 2 cut(s) 478, 1687
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 8 cut(s) 11, 33, 51, 75, 250, 1076, 1504, 2618
BslFI GGGAC 2 cut(s) 206, 779
BslI CCNNNNNNNGG 5 cut(s) 50, 1191, 1277, 2237, 2534
BsmAI GTCTC 6 cut(s) 228, 251, 879, 1654, 2010, 2707
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 2 cut(s) 206, 779
BsmI GAATGC 3 cut(s) 1200, 2327, 2629
BsnI GGCC 5 cut(s) 153, 293, 2368, 2797, 2820
Bso31I GGTCTC 3 cut(s) 228, 879, 1654
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 3 cut(s) 478, 1687, 2617
Bsp13I TCCGGA 2 cut(s) 249, 2617
Bsp1407I TGTACA 1 cut(s) 1152
BspACI CCGC 8 cut(s) 83, 241, 798, 1215, 1669, 1934, 2539, 2767
BspANI GGCC 5 cut(s) 153, 293, 2368, 2797, 2820
BspCNI CTCAG 3 cut(s) 349, 2122, 2476
BspEI TCCGGA 2 cut(s) 249, 2617
BspFNI CGCG 1 cut(s) 1770
BspHI TCATGA 2 cut(s) 792, 1543
BspLI GGNNCC 7 cut(s) 448, 600, 1481, 2167, 2547, 2616, 2775
BspMAI CTGCAG 4 cut(s) 1757, 1912, 2155, 2564
BspOI GCTAGC 1 cut(s) 1503
BspPI GGATC 6 cut(s) 166, 593, 606, 986, 1082, 2519
BspT107I GGYRCC 2 cut(s) 446, 2545
BspTI CTTAAG 1 cut(s) 2807
BspTNI GGTCTC 3 cut(s) 228, 879, 1654
BsrBI CCGCTC 1 cut(s) 798
BsrFI RCCGGY 2 cut(s) 50, 1503
BsrGI TGTACA 1 cut(s) 1152
BsrI ACTGG 5 cut(s) 122, 545, 1723, 2098, 2145
BssAI RCCGGY 2 cut(s) 50, 1503
BssECI CCNNGG 4 cut(s) 154, 218, 2074, 2491
BssNAI GTATAC 1 cut(s) 571
BssNI GRCGYC 1 cut(s) 244
BssT1I CCWWGG 1 cut(s) 154
Bst1107I GTATAC 1 cut(s) 571
Bst2UI CCWGG 6 cut(s) 1192, 1206, 1383, 2076, 2340, 2492
Bst4CI ACNGT 6 cut(s) 62, 863, 1157, 1747, 1802, 2260
Bst6I CTCTTC 5 cut(s) 116, 753, 882, 1920, 2013
BstACI GRCGYC 1 cut(s) 244
BstAFI CTTAAG 1 cut(s) 2807
BstAPI GCANNNNNTGC 1 cut(s) 1761
BstAUI TGTACA 1 cut(s) 1152
BstBAI YACGTR 1 cut(s) 888
BstC8I GCNNGC 7 cut(s) 52, 1501, 1505, 1714, 1766, 2124, 2747
BstDEI CTNAG 6 cut(s) 357, 2109, 2253, 2484, 2583, 2830
BstDSI CCRYGG 1 cut(s) 218
BstF5I GGATG 5 cut(s) 406, 419, 1181, 1291, 1533
BstFNI CGCG 1 cut(s) 1770
BstH2I RGCGCY 2 cut(s) 2425, 2806
BstHHI GCGC 4 cut(s) 1181, 1770, 2424, 2805
BstMAI GTCTC 6 cut(s) 228, 251, 879, 1654, 2010, 2707
BstMCI CGRYCG 1 cut(s) 2543
BstNI CCWGG 6 cut(s) 1192, 1206, 1383, 2076, 2340, 2492
BstNSI RCATGY 4 cut(s) 1123, 1136, 1250, 1716
BstSCI CCNGG 8 cut(s) 74, 1075, 1190, 1204, 1381, 2074, 2338, 2490
BstSFI CTRYAG 7 cut(s) 69, 862, 1753, 1908, 2151, 2370, 2560
BstSNI TACGTA 1 cut(s) 888
BstUI CGCG 1 cut(s) 1770
BstV1I GCAGC 8 cut(s) 169, 201, 1767, 1894, 1922, 2113, 2546, 2574
BstV2I GAAGAC 2 cut(s) 110, 1049
BstX2I RGATCY 3 cut(s) 158, 598, 1087
BstYI RGATCY 3 cut(s) 158, 598, 1087
BstZ17I GTATAC 1 cut(s) 571
BsuRI GGCC 5 cut(s) 153, 293, 2368, 2797, 2820
BtgI CCRYGG 1 cut(s) 218
BtgZI GCGATG 1 cut(s) 1622
BtsCI GGATG 5 cut(s) 406, 419, 1181, 1291, 1533
BtsI GCAGTG 2 cut(s) 2160, 2392
BtsIMutI CAGTG 5 cut(s) 1716, 2091, 2160, 2265, 2392
Cac8I GCNNGC 7 cut(s) 52, 1501, 1505, 1714, 1766, 2124, 2747
CciI TCATGA 2 cut(s) 792, 1543
CfoI GCGC 4 cut(s) 1181, 1770, 2424, 2805
Cfr10I RCCGGY 2 cut(s) 50, 1503
Cfr13I GGNCC 6 cut(s) 292, 465, 543, 2301, 2795, 2819
CseI GACGC 2 cut(s) 233, 1043
CsiI ACCWGGT 1 cut(s) 1190
Csp6I GTAC 9 cut(s) 740, 889, 1153, 1268, 1355, 2029, 2079, 2580, 2658
CviQI GTAC 9 cut(s) 740, 889, 1153, 1268, 1355, 2029, 2079, 2580, 2658
DdeI CTNAG 6 cut(s) 357, 2109, 2253, 2484, 2583, 2830
DraI TTTAAA 2 cut(s) 408, 1020
Eam1104I CTCTTC 5 cut(s) 116, 753, 882, 1920, 2013
EarI CTCTTC 5 cut(s) 116, 753, 882, 1920, 2013
Eco105I TACGTA 1 cut(s) 888
Eco130I CCWWGG 1 cut(s) 154
Eco147I AGGCCT 2 cut(s) 153, 2368
Eco24I GRGCYC 1 cut(s) 2617
Eco31I GGTCTC 3 cut(s) 228, 879, 1654
Eco47I GGWCC 3 cut(s) 465, 543, 2301
Eco57I CTGAAG 6 cut(s) 122, 314, 335, 399, 2037, 2528
Eco88I CYCGRG 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 2819
EcoRII CCWGG 6 cut(s) 1190, 1204, 1381, 2074, 2338, 2490
EcoT14I CCWWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 1812
EcoT38I GRGCYC 1 cut(s) 2617
ErhI CCWWGG 1 cut(s) 154
Esp3I CGTCTC 1 cut(s) 251
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1557, 1589
FaqI GGGAC 2 cut(s) 206, 779
FauNDI CATATG 5 cut(s) 336, 1738, 1760, 2287, 2314
FblI GTMKAC 2 cut(s) 570, 1362
FokI GGATG 5 cut(s) 406, 413, 1168, 1298, 1540
FriOI GRGCYC 1 cut(s) 2617
FspBI CTAG 6 cut(s) 387, 536, 735, 1407, 1500, 2529
GlaI GCGC 4 cut(s) 1180, 1769, 2423, 2804
GsaI CCCAGC 3 cut(s) 693, 873, 1335
GsuI CTGGAG 2 cut(s) 139, 612
HaeII RGCGCY 2 cut(s) 2425, 2806
HaeIII GGCC 5 cut(s) 153, 293, 2368, 2797, 2820
HapII CCGG 8 cut(s) 11, 33, 51, 75, 250, 1076, 1504, 2618
HgaI GACGC 2 cut(s) 233, 1043
HhaI GCGC 4 cut(s) 1181, 1770, 2424, 2805
Hin1I GRCGYC 1 cut(s) 244
Hin6I GCGC 4 cut(s) 1179, 1768, 2422, 2803
HinP1I GCGC 4 cut(s) 1179, 1768, 2422, 2803
HincII GTYRAC 3 cut(s) 136, 1459, 2691
HindII GTYRAC 3 cut(s) 136, 1459, 2691
HindIII AAGCTT 2 cut(s) 411, 633
HpaII CCGG 8 cut(s) 11, 33, 51, 75, 250, 1076, 1504, 2618
HphI GGTGA 5 cut(s) 1460, 1484, 1976, 1985, 2717
Hpy166II GTNNAC 7 cut(s) 136, 571, 729, 1252, 1363, 1459, 2691
Hpy8I GTNNAC 7 cut(s) 136, 571, 729, 1252, 1363, 1459, 2691
Hpy99I CGWCG 2 cut(s) 99, 146
HpyAV CCTTC 8 cut(s) 143, 260, 318, 1832, 2156, 2431, 2677, 2832
HpyCH4III ACNGT 6 cut(s) 62, 863, 1157, 1747, 1802, 2260
HpyCH4IV ACGT 2 cut(s) 195, 887
HpyF3I CTNAG 6 cut(s) 357, 2109, 2253, 2484, 2583, 2830
HpySE526I ACGT 2 cut(s) 195, 887
Hsp92I GRCGYC 1 cut(s) 244
HspAI GCGC 4 cut(s) 1179, 1768, 2422, 2803
Kpn2I TCCGGA 2 cut(s) 249, 2617
KroI GCCGGC 2 cut(s) 50, 1503
KroNI GCCGGC 2 cut(s) 52, 1505
LmnI GCTCC 5 cut(s) 481, 631, 1289, 1542, 2620
Lsp1109I GCAGC 8 cut(s) 169, 201, 1767, 1894, 1922, 2113, 2546, 2574
LweI GCATC 8 cut(s) 391, 428, 1190, 1518, 1656, 1687, 1819, 2192
MabI ACCWGGT 1 cut(s) 1190
MaeI CTAG 6 cut(s) 387, 536, 735, 1407, 1500, 2529
MaeII ACGT 2 cut(s) 195, 887
MaeIII GTNAC 7 cut(s) 781, 1081, 1274, 1396, 2112, 2260, 2590
MbiI CCGCTC 1 cut(s) 798
MfeI CAATTG 1 cut(s) 1441
MflI RGATCY 3 cut(s) 158, 598, 1087
MhlI GDGCHC 3 cut(s) 478, 1687, 2617
MlyI GAGTC 3 cut(s) 100, 131, 2257
MmeI TCCRAC 2 cut(s) 1323, 1670
Mph1103I ATGCAT 1 cut(s) 1812
MreI CGCCGGCG 1 cut(s) 50
MroI TCCGGA 2 cut(s) 249, 2617
MroNI GCCGGC 2 cut(s) 50, 1503
MroXI GAANNNNTTC 2 cut(s) 351, 552
MslI CAYNNNNRTG 5 cut(s) 377, 444, 976, 1106, 1173
MspA1I CMGCKG 1 cut(s) 2126
MspCI CTTAAG 1 cut(s) 2807
MspI CCGG 8 cut(s) 11, 33, 51, 75, 250, 1076, 1504, 2618
MspR9I CCNGG 8 cut(s) 76, 1077, 1192, 1206, 1383, 2076, 2340, 2492
MunI CAATTG 1 cut(s) 1441
Mva1269I GAATGC 3 cut(s) 1200, 2327, 2629
MvaI CCWGG 6 cut(s) 1192, 1206, 1383, 2076, 2340, 2492
MvnI CGCG 1 cut(s) 1770
NaeI GCCGGC 2 cut(s) 52, 1505
NciI CCSGG 2 cut(s) 76, 1077
NdeI CATATG 5 cut(s) 336, 1738, 1760, 2287, 2314
NgoMIV GCCGGC 2 cut(s) 50, 1503
NheI GCTAGC 1 cut(s) 1499
NlaIV GGNNCC 7 cut(s) 448, 600, 1481, 2167, 2547, 2616, 2775
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 4 cut(s) 781, 1274, 2112, 2260
NsiI ATGCAT 1 cut(s) 1812
NspI RCATGY 4 cut(s) 1123, 1136, 1250, 1716
PaeI GCATGC 1 cut(s) 1716
PagI TCATGA 2 cut(s) 792, 1543
PceI AGGCCT 2 cut(s) 153, 2368
PciI ACATGT 1 cut(s) 1246
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 3 cut(s) 1200, 2327, 2629
PdiI GCCGGC 2 cut(s) 52, 1505
PdmI GAANNNNTTC 2 cut(s) 351, 552
PfeI GAWTC 9 cut(s) 452, 1024, 1425, 1601, 1951, 2393, 2399, 2573, 2702
PflFI GACNNNGTC 2 cut(s) 140, 779
PflMI CCANNNNNTGG 1 cut(s) 1191
PfoI TCCNGGA 1 cut(s) 2338
PleI GAGTC 3 cut(s) 100, 131, 2257
PpsI GAGTC 3 cut(s) 100, 131, 2257
Ppu21I YACGTR 1 cut(s) 888
PscI ACATGT 1 cut(s) 1246
PshBI ATTAAT 1 cut(s) 1997
PsiI TTATAA 1 cut(s) 2456
Psp6I CCWGG 6 cut(s) 1190, 1204, 1381, 2074, 2338, 2490
PspFI CCCAGC 3 cut(s) 689, 869, 1331
PspGI CCWGG 6 cut(s) 1190, 1204, 1381, 2074, 2338, 2490
PspN4I GGNNCC 7 cut(s) 448, 600, 1481, 2167, 2547, 2616, 2775
PspPI GGNCC 6 cut(s) 292, 465, 543, 2301, 2795, 2819
PsrI GAACNNNNNNTAC 2 cut(s) 1338, 1370
PstI CTGCAG 4 cut(s) 1757, 1912, 2155, 2564
PsuI RGATCY 3 cut(s) 158, 598, 1087
PsyI GACNNNGTC 2 cut(s) 140, 779
PvuII CAGCTG 1 cut(s) 2126
RsaI GTAC 9 cut(s) 741, 890, 1154, 1269, 1356, 2030, 2080, 2581, 2659
RsaNI GTAC 9 cut(s) 740, 889, 1153, 1268, 1355, 2029, 2079, 2580, 2658
RseI CAYNNNNRTG 5 cut(s) 377, 444, 976, 1106, 1173
Sau96I GGNCC 6 cut(s) 292, 465, 543, 2301, 2795, 2819
ScaI AGTACT 1 cut(s) 2581
SchI GAGTC 3 cut(s) 100, 131, 2257
ScrFI CCNGG 8 cut(s) 76, 1077, 1192, 1206, 1383, 2076, 2340, 2492
SduI GDGCHC 3 cut(s) 478, 1687, 2617
SexAI ACCWGGT 1 cut(s) 1190
SfaNI GCATC 8 cut(s) 391, 428, 1190, 1518, 1656, 1687, 1819, 2192
SfcI CTRYAG 7 cut(s) 69, 862, 1753, 1908, 2151, 2370, 2560
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 3 cut(s) 465, 543, 2301
SmiMI CAYNNNNRTG 5 cut(s) 377, 444, 976, 1106, 1173
SmlI CTYRAG 5 cut(s) 229, 2213, 2326, 2750, 2807
SmoI CTYRAG 5 cut(s) 229, 2213, 2326, 2750, 2807
SnaBI TACGTA 1 cut(s) 888
SphI GCATGC 1 cut(s) 1716
SseBI AGGCCT 2 cut(s) 153, 2368
SsiI CCGC 8 cut(s) 83, 241, 798, 1215, 1669, 1934, 2539, 2767
SspMI CTAG 6 cut(s) 387, 536, 735, 1407, 1500, 2529
StuI AGGCCT 2 cut(s) 153, 2368
StyD4I CCNGG 8 cut(s) 74, 1075, 1190, 1204, 1381, 2074, 2338, 2490
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 6 cut(s) 62, 863, 1157, 1747, 1802, 2260
TaiI ACGT 2 cut(s) 198, 890
TaqI TCGA 1 cut(s) 825
TaqII GACCGA 3 cut(s) 453, 560, 2289
TatI WGTACW 3 cut(s) 739, 1152, 2579
TauI GCSGC 3 cut(s) 244, 1217, 2769
TfiI GAWTC 9 cut(s) 452, 1024, 1425, 1601, 1951, 2393, 2399, 2573, 2702
TscAI CASTG 5 cut(s) 1723, 2098, 2160, 2265, 2392
TseFI GTSAC 4 cut(s) 781, 1274, 2112, 2260
TseI GCWGC 8 cut(s) 182, 214, 1755, 1907, 1910, 2126, 2559, 2562
Tsp45I GTSAC 4 cut(s) 781, 1274, 2112, 2260
TspDTI ATGAA 9 cut(s) 781, 966, 1560, 1715, 1977, 2163, 2334, 2421, 2712
TspGWI ACGGA 4 cut(s) 83, 130, 806, 1293
TspRI CASTG 5 cut(s) 1723, 2098, 2160, 2265, 2392
Tth111I GACNNNGTC 2 cut(s) 140, 779
Van91I CCANNNNNTGG 1 cut(s) 1191
Vha464I CTTAAG 1 cut(s) 2807
VpaK11BI GGWCC 3 cut(s) 465, 543, 2301
VspI ATTAAT 1 cut(s) 1997
XapI RAATTY 8 cut(s) 308, 518, 552, 902, 1015, 1919, 2051, 2269
XbaI TCTAGA 2 cut(s) 386, 535
XceI RCATGY 4 cut(s) 1123, 1136, 1250, 1716
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XmiI GTMKAC 2 cut(s) 570, 1362
XmnI GAANNNNTTC 2 cut(s) 351, 552
XspI CTAG 6 cut(s) 387, 536, 735, 1407, 1500, 2529
ZrmI AGTACT 1 cut(s) 2581
Zsp2I ATGCAT 1 cut(s) 1812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.