Rroxscaffold_3G00247560

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007240
Physical Location & Seq
Reverse (-)
40867699 .. 40874348
6650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247560.1

Sequence Viewer

Length: 2619 bp
ATGGTGGAAGGTAGGGTTTGTTTGTCCAAAGAGGCCCAAAATGGATTAGAATTTCTGAAGCGAAGAAGGCTTCAGCATATGAGATCAGAAACTATTCCTGAGCCTGTAAGTTTCACGAACATGATGTCTAGAAGTGGAGGGGATGCTTTAAAAGCTTCAGCATCCTGTGGTGTTAGAATACATGGAGGTGCCGATTCAGTTTCCCGGTCCAATGGTGCTCCACAGGGGAAGGATGTTTTTTCAAAGCGTAAGGTGGATAAATTTGAAACAAGTGATCTAGGCTGGACCGAGAAAATTCCCGAGTGTCCTGTATACCATCCAGCAAAGGAGGAGTTTGAGGATCCTTTAGTTTATTTACAGAAAATAGCTCCAGAAGCTTCCAAATATGGTATATGCAAGATTGTCTCCCCTGTTAGTGCTTCTACACCTGCTGGGGTTGTATTGATGAGGGAAAAAGCAGGGTTTAAGTTCACTACTAGAGTACAACCTCTTCGCCTTGCTGAGTGGGACAATGATGACAGGGTCACTTTTTTCATGAGCGGAAGAAACTATACATTCCGTGATTTCGAGAAAATGGCGAACAAGGTTTTTGCTCGTAGATACTGTAGTGCTGGGTCTCTTCCTCCTACGTACTTGGAAAAAGAATTTTGGAAAGAAATTGGTTCTGGAAAGACAGAAAGTGTTGAATATGCCTGTGATGTAGATGGTAGTGCATTTTCATGTTCTCCCAATGATCCACTTGGAAGTAGTAAATGGAATTTAAAGAATCTTTCAAGGCTACCCAAGTCTATTCTGCGTCTTCTGGAAAATGCAATTCCGGGTGTAACAGATCCTATGCTATACATTGGAATGATATTTAGCATGTTTGCTTGGCATGTGGAAGATCACTATTTGTACAGTATCAATTATCATCATTGTGGCGCATCCAAAACATGGTATGGCATTCCAGGTCATGCCGCCCTGCAATTTGAAAAGGTTGTCAAGGAACATGTTTACACCAATGATATTGTACCAAGTGATGGAGAGGATGGAGCTTTTGATGTCTTATTGGGGAAAACAACTCTGTTTCCTCCCAGCATATTGTTGGAACATGATGTACCTGTCTACAAAGCTGTGCAAAAGCCAGGAGAGTTTGTGGTTACTTTCCCTAGAGCGTATCATGCTGGATTCAGTCATGGTTTCAATTGTGGTGAGGCAGTCAACTTTGCTATTGGTGATTGGTTCCCATTGGGTGCTATTGCTAGCCGGCGTTATGCACTTCTTAATAGGATGCCTCTGCTTCCTCATGAAGAACTCCTGTGCAAAGAAGCCATGCTTCTCCATACAAGTTTAGAGCTTGAAGATTCAGATTATTCTTCTGCTGACTTGGTTTCCCATCATCGCATTAAGGTCTCTTTTGTGAAACTGATGCAGTTTCAGCATCGTGCTCGTTGGACTTTAATGAAGTCTGGGGCATGCACTGGTGTCTTGCCAAACTCTTATGGAACTGTTCTCTGCTGCATATGCAAGCGCGATTGCTATCTTGCCTACATTAGTTGCAACTGTTATATGCATCATGTGTGCCTTCGCCATGATGTAAGGTCATTGGACTTGTCATGTCAGAGCAATCCTACTCTCTTTTTAAGGGAGGAGATAGCAGAAATGGAAGCTGCAGCAAGTAAATTTGAAGAGGAAGTGGAAGTGATAGAGGAGATTCAAGGTCAAGGTGAAAATGGTGATGACTTGTATTCATATCCATTAATTTTGCCTCCGATAGCTGAAGAGAAAGGGTACTCCCCTTACTGCAATATAAAATTTGAGTTGAACCCCGAACTCCTTGGTACAACAGAAATCACTGGCACAACTCTGGCTCAGTCACAAGAGCCAGCTGCACTTGGTTCTCATACCAGTCCTGCAGTGTCAGAAGGTTCCATTTCATGTATTGCATCTACTCTTTGTTCTCTTTCAGAGCCACTTGAGAGCTTGTATGCACCCAATAATGGAATTGCCAACTCTAAGACAGTGACTCTAAATTCCAAAAGGTCTGCATATGAGTCTTCTCGGTCCTCCCCATCATATGATGAATGCTCAAGTGTATGTCCAGGAAGTTCTAACGCATTGGAGGTTAGGCCTATAGTAGATCAAGGCAGTGATGATTCTGATTCAGAGATATTTAGGGTTAAGCGCCGTTCTTCATTGAAGGTGGAGAGAAGAATTATAAATGATGTTTTGCCATCAAATCATTCTGAGAACCAGCAGGGATTTAAGCGACTAAAGAAACTGCAACCTGATCCTAGATGTGGGCGGTCGGTGCCATTACAAAGCTGCAGCACTACGGAATCAAGTACTAAGTTGGTTACAACTACTAATAGCAAAGGGACTCCGGAGAATGCTTTAAGAGATGACAGGTTTGCCAGAGGAAGTACCATATCCAGAGGAAGTACCATTCCCTTCTCTATCAAGTTTAAGAAGTTGACCAATGAAGATTCTGTTAGGAGACAGCGAGATCACCACAGAACTGATATACGCCAGCTTGAGTTGGGAAAGAGCCGCAGGGAACCTCCCCCAATAGAGATTGGGCCAAAGCGCCTTAAGGTCAAAGGCCCTTCTTTCTTAGCTTCGGAGAACAGGTTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0000792 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010016 GO:0010033 GO:0010228 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032451 GO:0032452 GO:0032454 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045815 GO:0048366 GO:0048367 GO:0048439 GO:0048518 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090567 GO:0090698 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

872

Amino Acids

97.22

Weight (kDa)

7.08

Isoelectric Point (pI)

56.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 103 - 135 1.1e-14 jmjN domain
JmjC PF02373 279 - 402 2.2e-39 JmjC domain, hydroxylase
zf-C5HC2 PF02928 499 - 550 2e-08 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2198
AarI CACCTGC 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 2 cut(s) 188, 2290
AccB7I CCANNNNNTGG 2 cut(s) 933, 1019
AccBSI CCGCTC 1 cut(s) 540
AccI GTMKAC 2 cut(s) 312, 1104
AccII CGCG 1 cut(s) 1512
AccIII TCCGGA 1 cut(s) 2362
AciI CCGC 4 cut(s) 540, 957, 2284, 2530
AclWI GGATC 5 cut(s) 335, 348, 728, 824, 2264
AcsI RAATTY 8 cut(s) 50, 260, 294, 644, 757, 1661, 1793, 2011
AcuI CTGAAG 4 cut(s) 56, 77, 141, 1779
AfiI CCNNNNNNNGG 4 cut(s) 933, 1019, 1979, 2279
AflII CTTAAG 1 cut(s) 2570
AflIII ACRYGT 1 cut(s) 988
AjnI CCWGG 3 cut(s) 946, 1123, 2080
Alw21I GWGCWC 2 cut(s) 220, 1429
Alw26I GTCTC 4 cut(s) 409, 621, 1396, 2470
AlwI GGATC 5 cut(s) 335, 348, 728, 824, 2264
Ama87I CYCGRG 1 cut(s) 299
Aor13HI TCCGGA 1 cut(s) 2362
AoxI GGCC 4 cut(s) 33, 2108, 2558, 2581
ApeKI GCWGC 6 cut(s) 1497, 1649, 1652, 1868, 2304, 2307
ApoI RAATTY 8 cut(s) 50, 260, 294, 644, 757, 1661, 1793, 2011
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AseI ATTAAT 1 cut(s) 1739
Asp700I GAANNNNTTC 1 cut(s) 93
AspLEI GCGC 4 cut(s) 923, 1512, 2166, 2568
AspS9I GGNCC 6 cut(s) 34, 207, 285, 2043, 2558, 2582
AsuC2I CCSGG 2 cut(s) 205, 819
AsuHPI GGTGA 5 cut(s) 1202, 1226, 1718, 1727, 2480
AsuNHI GCTAGC 1 cut(s) 1241
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 3 cut(s) 207, 285, 2043
BaeI ACNNNNGTAYC 2 cut(s) 592, 625
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 188, 2290
BarI GAAGNNNNNNTAC 4 cut(s) 474, 506, 535, 567
BbsI GAAGAC 2 cut(s) 791, 2028
Bbv12I GWGCWC 2 cut(s) 220, 1429
BbvI GCAGC 6 cut(s) 1484, 1636, 1664, 1855, 2291, 2319
BccI CCATC 7 cut(s) 324, 698, 1013, 1022, 1383, 2059, 2221
BceAI ACGGC 1 cut(s) 2151
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 3 cut(s) 948, 1125, 2082
BcnI CCSGG 2 cut(s) 205, 819
BcoDI GTCTC 4 cut(s) 409, 621, 1396, 2470
BfaI CTAG 6 cut(s) 129, 278, 477, 1149, 1242, 2274
BfmI CTRYAG 5 cut(s) 604, 1650, 1893, 2112, 2305
BfoI RGCGCY 2 cut(s) 2167, 2569
BfrI CTTAAG 1 cut(s) 2570
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 8 cut(s) 957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
BlsI GCNGC 8 cut(s) 958, 1499, 1651, 1654, 1870, 2306, 2309, 2531
BmcAI AGTACT 1 cut(s) 2326
Bme1390I CCNGG 5 cut(s) 205, 819, 948, 1125, 2082
Bme18I GGWCC 3 cut(s) 207, 285, 2043
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 6 cut(s) 34, 207, 285, 2043, 2558, 2582
BmiI GGNNCC 6 cut(s) 190, 342, 1223, 1909, 2292, 2538
BmrFI CCNGG 5 cut(s) 205, 819, 948, 1125, 2082
BmsI GCATC 8 cut(s) 133, 170, 932, 1260, 1398, 1429, 1561, 1934
BmtI GCTAGC 1 cut(s) 1245
BpiI GAAGAC 2 cut(s) 791, 2028
BpmI CTGGAG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 3 cut(s) 1976, 2053, 2534
BpuMI CCSGG 2 cut(s) 205, 819
BsaAI YACGTR 1 cut(s) 630
BsaBI GATNNNNATC 1 cut(s) 1518
BsaI GGTCTC 2 cut(s) 621, 1396
BsaJI CCNNGG 1 cut(s) 1816
BsaWI WCCGGW 1 cut(s) 2362
BsaXI ACNNNNNCTCC 3 cut(s) 1119, 1149, 2594
Bsc4I CCNNNNNNNGG 4 cut(s) 933, 1019, 1979, 2279
Bse118I RCCGGY 1 cut(s) 1245
Bse1I ACTGG 3 cut(s) 1465, 1840, 1887
Bse8I GATNNNNATC 1 cut(s) 1518
BseAI TCCGGA 1 cut(s) 2362
BseBI CCWGG 3 cut(s) 948, 1125, 2082
BseDI CCNNGG 1 cut(s) 1816
BseGI GGATG 7 cut(s) 148, 161, 238, 316, 923, 1033, 1275
BseJI GATNNNNATC 1 cut(s) 1518
BseLI CCNNNNNNNGG 4 cut(s) 933, 1019, 1979, 2279
BseMII CTCAG 4 cut(s) 90, 492, 1865, 2217
BseNI ACTGG 3 cut(s) 1465, 1840, 1887
BseRI GAGGAG 3 cut(s) 344, 1643, 1703
BseXI GCAGC 6 cut(s) 1484, 1636, 1664, 1855, 2291, 2319
BseYI CCCAGC 3 cut(s) 431, 611, 1073
BsgI GTGCAG 1 cut(s) 1854
Bsh1236I CGCG 1 cut(s) 1512
Bsh1285I CGRYCG 1 cut(s) 2288
BshFI GGCC 4 cut(s) 35, 2110, 2560, 2583
BshNI GGYRCC 2 cut(s) 188, 2290
BsiEI CGRYCG 1 cut(s) 2288
BsiHKAI GWGCWC 2 cut(s) 220, 1429
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 205, 818, 1246, 2363
BslFI GGGAC 2 cut(s) 521, 2371
BslI CCNNNNNNNGG 4 cut(s) 933, 1019, 1979, 2279
BsmAI GTCTC 4 cut(s) 409, 621, 1396, 2470
BsmFI GGGAC 2 cut(s) 521, 2371
BsmI GAATGC 3 cut(s) 942, 2069, 2374
BsnI GGCC 4 cut(s) 35, 2110, 2560, 2583
Bso31I GGTCTC 2 cut(s) 621, 1396
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 220, 1429
Bsp13I TCCGGA 1 cut(s) 2362
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
BspACI CCGC 4 cut(s) 540, 957, 2284, 2530
BspANI GGCC 4 cut(s) 35, 2110, 2560, 2583
BspCNI CTCAG 4 cut(s) 91, 493, 1864, 2218
BspEI TCCGGA 1 cut(s) 2362
BspFNI CGCG 1 cut(s) 1512
BspHI TCATGA 2 cut(s) 534, 1285
BspLI GGNNCC 6 cut(s) 190, 342, 1223, 1909, 2292, 2538
BspMAI CTGCAG 3 cut(s) 1654, 1897, 2309
BspMI ACCTGC 1 cut(s) 436
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 5 cut(s) 335, 348, 728, 824, 2264
BspT107I GGYRCC 2 cut(s) 188, 2290
BspTI CTTAAG 1 cut(s) 2570
BspTNI GGTCTC 2 cut(s) 621, 1396
BsrBI CCGCTC 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 1245
BsrGI TGTACA 1 cut(s) 894
BsrI ACTGG 3 cut(s) 1465, 1840, 1887
BssAI RCCGGY 1 cut(s) 1245
BssECI CCNNGG 1 cut(s) 1816
BssMI GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
BssNAI GTATAC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 1816
Bst1107I GTATAC 1 cut(s) 313
Bst2UI CCWGG 3 cut(s) 948, 1125, 2082
Bst4CI ACNGT 5 cut(s) 605, 899, 1489, 1544, 2002
Bst6I CTCTTC 4 cut(s) 495, 624, 1662, 1755
BstAFI CTTAAG 1 cut(s) 2570
BstAUI TGTACA 1 cut(s) 894
BstBAI YACGTR 1 cut(s) 630
BstC8I GCNNGC 6 cut(s) 1243, 1247, 1456, 1508, 1866, 2510
BstDEI CTNAG 7 cut(s) 99, 501, 1851, 1995, 2226, 2328, 2593
BstF5I GGATG 7 cut(s) 148, 161, 238, 316, 923, 1033, 1275
BstFNI CGCG 1 cut(s) 1512
BstH2I RGCGCY 2 cut(s) 2167, 2569
BstHHI GCGC 4 cut(s) 923, 1512, 2166, 2568
BstKTI GATC 9 cut(s) 86, 277, 343, 736, 832, 886, 2122, 2272, 2488
BstMAI GTCTC 4 cut(s) 409, 621, 1396, 2470
BstMBI GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
BstMCI CGRYCG 1 cut(s) 2288
BstMWI GCNNNNNNNGC 7 cut(s) 67, 152, 374, 1160, 1417, 1503, 2290
BstNI CCWGG 3 cut(s) 948, 1125, 2082
BstNSI RCATGY 4 cut(s) 865, 878, 992, 1458
BstSCI CCNGG 5 cut(s) 203, 817, 946, 1123, 2080
BstSFI CTRYAG 5 cut(s) 604, 1650, 1893, 2112, 2305
BstSNI TACGTA 1 cut(s) 630
BstUI CGCG 1 cut(s) 1512
BstV1I GCAGC 6 cut(s) 1484, 1636, 1664, 1855, 2291, 2319
BstV2I GAAGAC 2 cut(s) 791, 2028
BstX2I RGATCY 2 cut(s) 340, 829
BstYI RGATCY 2 cut(s) 340, 829
BstZ17I GTATAC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 35, 2110, 2560, 2583
BtgZI GCGATG 1 cut(s) 1364
BtsCI GGATG 7 cut(s) 148, 161, 238, 316, 923, 1033, 1275
BtsI GCAGTG 2 cut(s) 1902, 2134
BtsIMutI CAGTG 5 cut(s) 1458, 1833, 1902, 2007, 2134
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 6 cut(s) 1243, 1247, 1456, 1508, 1866, 2510
CciI TCATGA 2 cut(s) 534, 1285
CfoI GCGC 4 cut(s) 923, 1512, 2166, 2568
Cfr10I RCCGGY 1 cut(s) 1245
Cfr13I GGNCC 6 cut(s) 34, 207, 285, 2043, 2558, 2582
CseI GACGC 1 cut(s) 785
DdeI CTNAG 7 cut(s) 99, 501, 1851, 1995, 2226, 2328, 2593
DpnI GATC 9 cut(s) 85, 276, 342, 735, 831, 885, 2121, 2271, 2487
DpnII GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
DraI TTTAAA 2 cut(s) 150, 762
Eam1104I CTCTTC 4 cut(s) 495, 624, 1662, 1755
EarI CTCTTC 4 cut(s) 495, 624, 1662, 1755
Eco105I TACGTA 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 1816
Eco147I AGGCCT 1 cut(s) 2110
Eco31I GGTCTC 2 cut(s) 621, 1396
Eco47I GGWCC 3 cut(s) 207, 285, 2043
Eco57I CTGAAG 4 cut(s) 56, 77, 141, 1779
Eco88I CYCGRG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 2582
EcoRII CCWGG 3 cut(s) 946, 1123, 2080
EcoT14I CCWWGG 1 cut(s) 1816
EcoT22I ATGCAT 1 cut(s) 1554
ErhI CCWWGG 1 cut(s) 1816
FalI AAGNNNNNCTT 2 cut(s) 1299, 1331
FaqI GGGAC 2 cut(s) 521, 2371
FauNDI CATATG 4 cut(s) 78, 1502, 2029, 2056
FblI GTMKAC 2 cut(s) 312, 1104
Fnu4HI GCNGC 8 cut(s) 957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
FokI GGATG 7 cut(s) 148, 155, 245, 303, 910, 1040, 1282
Fsp4HI GCNGC 8 cut(s) 957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
FspBI CTAG 6 cut(s) 129, 278, 477, 1149, 1242, 2274
GlaI GCGC 4 cut(s) 922, 1511, 2165, 2567
GluI GCNGC 8 cut(s) 957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
GsaI CCCAGC 3 cut(s) 435, 615, 1077
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 2 cut(s) 2167, 2569
HaeIII GGCC 4 cut(s) 35, 2110, 2560, 2583
HapII CCGG 4 cut(s) 205, 818, 1246, 2363
HgaI GACGC 1 cut(s) 785
HhaI GCGC 4 cut(s) 923, 1512, 2166, 2568
Hin6I GCGC 4 cut(s) 921, 1510, 2164, 2566
HinP1I GCGC 4 cut(s) 921, 1510, 2164, 2566
HincII GTYRAC 2 cut(s) 1201, 2454
HindII GTYRAC 2 cut(s) 1201, 2454
HindIII AAGCTT 2 cut(s) 153, 375
HpaII CCGG 4 cut(s) 205, 818, 1246, 2363
HphI GGTGA 5 cut(s) 1202, 1226, 1718, 1727, 2480
Hpy166II GTNNAC 6 cut(s) 313, 471, 994, 1105, 1201, 2454
Hpy8I GTNNAC 6 cut(s) 313, 471, 994, 1105, 1201, 2454
HpyAV CCTTC 7 cut(s) 60, 223, 1574, 1898, 2173, 2440, 2595
HpyCH4III ACNGT 5 cut(s) 605, 899, 1489, 1544, 2002
HpyCH4IV ACGT 1 cut(s) 629
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 152, 374, 1160, 1417, 1503, 2290
HpyF3I CTNAG 7 cut(s) 99, 501, 1851, 1995, 2226, 2328, 2593
HpySE526I ACGT 1 cut(s) 629
HspAI GCGC 4 cut(s) 921, 1510, 2164, 2566
Kpn2I TCCGGA 1 cut(s) 2362
KroI GCCGGC 1 cut(s) 1245
KroNI GCCGGC 1 cut(s) 1247
Kzo9I GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
LmnI GCTCC 3 cut(s) 223, 373, 1031
Lsp1109I GCAGC 6 cut(s) 1484, 1636, 1664, 1855, 2291, 2319
LweI GCATC 8 cut(s) 133, 170, 932, 1260, 1398, 1429, 1561, 1934
MaeI CTAG 6 cut(s) 129, 278, 477, 1149, 1242, 2274
MaeII ACGT 1 cut(s) 629
MaeIII GTNAC 6 cut(s) 523, 823, 1138, 1854, 2002, 2335
MalI GATC 9 cut(s) 85, 276, 342, 735, 831, 885, 2121, 2271, 2487
MbiI CCGCTC 1 cut(s) 540
MboI GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
MfeI CAATTG 1 cut(s) 1183
MflI RGATCY 2 cut(s) 340, 829
MhlI GDGCHC 2 cut(s) 220, 1429
MlyI GAGTC 3 cut(s) 1999, 2042, 2353
MmeI TCCRAC 2 cut(s) 1065, 1412
Mph1103I ATGCAT 1 cut(s) 1554
MroI TCCGGA 1 cut(s) 2362
MroNI GCCGGC 1 cut(s) 1245
MroXI GAANNNNTTC 1 cut(s) 93
MslI CAYNNNNRTG 5 cut(s) 119, 186, 718, 848, 915
MspA1I CMGCKG 1 cut(s) 1868
MspCI CTTAAG 1 cut(s) 2570
MspI CCGG 4 cut(s) 205, 818, 1246, 2363
MspR9I CCNGG 5 cut(s) 205, 819, 948, 1125, 2082
MunI CAATTG 1 cut(s) 1183
Mva1269I GAATGC 3 cut(s) 942, 2069, 2374
MvaI CCWGG 3 cut(s) 948, 1125, 2082
MvnI CGCG 1 cut(s) 1512
MwoI GCNNNNNNNGC 7 cut(s) 67, 152, 374, 1160, 1417, 1503, 2290
NaeI GCCGGC 1 cut(s) 1247
NciI CCSGG 2 cut(s) 205, 819
NdeI CATATG 4 cut(s) 78, 1502, 2029, 2056
NdeII GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
NgoMIV GCCGGC 1 cut(s) 1245
NheI GCTAGC 1 cut(s) 1241
NlaIV GGNNCC 6 cut(s) 190, 342, 1223, 1909, 2292, 2538
NmuCI GTSAC 3 cut(s) 523, 1854, 2002
NsiI ATGCAT 1 cut(s) 1554
NspI RCATGY 4 cut(s) 865, 878, 992, 1458
PaeI GCATGC 1 cut(s) 1458
PagI TCATGA 2 cut(s) 534, 1285
PaqCI CACCTGC 1 cut(s) 436
PceI AGGCCT 1 cut(s) 2110
PciI ACATGT 1 cut(s) 988
PctI GAATGC 3 cut(s) 942, 2069, 2374
PdiI GCCGGC 1 cut(s) 1247
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 9 cut(s) 194, 766, 1167, 1343, 1693, 2135, 2141, 2318, 2465
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 2 cut(s) 933, 1019
PfoI TCCNGGA 1 cut(s) 2080
PkrI GCNGC 8 cut(s) 958, 1499, 1651, 1654, 1870, 2306, 2309, 2531
PleI GAGTC 3 cut(s) 1999, 2041, 2353
PpsI GAGTC 3 cut(s) 1999, 2041, 2353
Ppu21I YACGTR 1 cut(s) 630
PscI ACATGT 1 cut(s) 988
PshBI ATTAAT 1 cut(s) 1739
PsiI TTATAA 1 cut(s) 2198
Psp6I CCWGG 3 cut(s) 946, 1123, 2080
PspFI CCCAGC 3 cut(s) 431, 611, 1073
PspGI CCWGG 3 cut(s) 946, 1123, 2080
PspN4I GGNNCC 6 cut(s) 190, 342, 1223, 1909, 2292, 2538
PspPI GGNCC 6 cut(s) 34, 207, 285, 2043, 2558, 2582
PsrI GAACNNNNNNTAC 2 cut(s) 1080, 1112
PstI CTGCAG 3 cut(s) 1654, 1897, 2309
PsuI RGATCY 2 cut(s) 340, 829
PsyI GACNNNGTC 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 1868
RseI CAYNNNNRTG 5 cut(s) 119, 186, 718, 848, 915
SatI GCNGC 8 cut(s) 957, 1498, 1650, 1653, 1869, 2305, 2308, 2530
Sau3AI GATC 9 cut(s) 83, 274, 340, 733, 829, 883, 2119, 2269, 2485
Sau96I GGNCC 6 cut(s) 34, 207, 285, 2043, 2558, 2582
ScaI AGTACT 1 cut(s) 2326
SchI GAGTC 3 cut(s) 1999, 2042, 2353
ScrFI CCNGG 5 cut(s) 205, 819, 948, 1125, 2082
SduI GDGCHC 2 cut(s) 220, 1429
SfaNI GCATC 8 cut(s) 133, 170, 932, 1260, 1398, 1429, 1561, 1934
SfcI CTRYAG 5 cut(s) 604, 1650, 1893, 2112, 2305
SinI GGWCC 3 cut(s) 207, 285, 2043
SmiMI CAYNNNNRTG 5 cut(s) 119, 186, 718, 848, 915
SmlI CTYRAG 4 cut(s) 1955, 2068, 2513, 2570
SmoI CTYRAG 4 cut(s) 1955, 2068, 2513, 2570
SnaBI TACGTA 1 cut(s) 630
SphI GCATGC 1 cut(s) 1458
SseBI AGGCCT 1 cut(s) 2110
SsiI CCGC 4 cut(s) 540, 957, 2284, 2530
SspMI CTAG 6 cut(s) 129, 278, 477, 1149, 1242, 2274
StuI AGGCCT 1 cut(s) 2110
StyD4I CCNGG 5 cut(s) 203, 817, 946, 1123, 2080
StyI CCWWGG 1 cut(s) 1816
TaaI ACNGT 5 cut(s) 605, 899, 1489, 1544, 2002
TaiI ACGT 1 cut(s) 632
TaqI TCGA 1 cut(s) 567
TaqII GACCGA 2 cut(s) 302, 2031
TatI WGTACW 3 cut(s) 481, 894, 2324
TauI GCSGC 2 cut(s) 959, 2532
TfiI GAWTC 9 cut(s) 194, 766, 1167, 1343, 1693, 2135, 2141, 2318, 2465
TscAI CASTG 5 cut(s) 1465, 1840, 1902, 2007, 2134
TseFI GTSAC 3 cut(s) 523, 1854, 2002
TseI GCWGC 6 cut(s) 1497, 1649, 1652, 1868, 2304, 2307
Tsp45I GTSAC 3 cut(s) 523, 1854, 2002
TspDTI ATGAA 9 cut(s) 523, 708, 1302, 1457, 1719, 1905, 2076, 2163, 2475
TspGWI ACGGA 2 cut(s) 548, 2330
TspRI CASTG 5 cut(s) 1465, 1840, 1902, 2007, 2134
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 2 cut(s) 933, 1019
Vha464I CTTAAG 1 cut(s) 2570
VpaK11BI GGWCC 3 cut(s) 207, 285, 2043
VspI ATTAAT 1 cut(s) 1739
XapI RAATTY 8 cut(s) 50, 260, 294, 644, 757, 1661, 1793, 2011
XbaI TCTAGA 1 cut(s) 128
XceI RCATGY 4 cut(s) 865, 878, 992, 1458
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmiI GTMKAC 2 cut(s) 312, 1104
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 6 cut(s) 129, 278, 477, 1149, 1242, 2274
ZrmI AGTACT 1 cut(s) 2326
Zsp2I ATGCAT 1 cut(s) 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.