MD06G1027100.v1.1

Protein of unknown function (DUF760)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014092
Physical Location & Seq
Forward (+)
3337571 .. 3341030
3460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1027100.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGCAGGTTTCAGGGGTAATTGGCGACGTTTCTTTTGTAATTCCCTCCGGTGGTGGTCTCAGGTGGCCAGAATTCGGACATTTCCATGCCCACGGCCAAAACCATTCACATCCACTCACTGATTCCAAGTCATTTCTTTCAGCTTCATTTTTCAGAAGTTGTCCTTCATTTTGCATCCCAAAGCTGGGAAACGGACTATATAACTGCCGAGCAAGGGGGTTGGTTGTGAGAGCATCAAAGGATTCAAGTGATAATTTGGTGCCAGTTGCTCCTCTTCAATTTGAATCTCCCGCTGGCCAGCTTTTGGCCCAAATATTGCAAACTCATCCACATCTACTACCTGCAGCCATTGATCAGCAGCTTGAGAACCTTCAAATAGATAAAGATGCCCAAAGAAAAGAAGCTGCTTCATCTGCAAATCTCCTCTACAAACGAATAGCTAAAGTCAAGGATAAAGAAAGGCGCATGGCATTGGAAGAGATAATATACTGCTTGATAGTGCATAAATTCGTAGACCGTGAAATTTCAATGATCCCGAAGATATCAGAGACATCAGATCCCACCGGTAGAGTGGATTTCTGGCCAAACCAAGAAAAGAAACTAGAGTCTGTTCACTCTCCTGAAGCATATGAGATGATACAGAGCCACTTATCCCTTGTACTTGGTGAGCGACTTGTGGGCCCCCTTTCTTCCATGGTTGAGATTAGCAAAATAAAACTTGGAAAGCTGTATGCTGCTTCAATAATGTACGGATATTTTCTCAAAAGAGTTGATGAGCGGTTCCAGCTTGAGAGAACCATGAACACCCTTCCTGAGGGTTTCACTGCAGATCCAGCACCAGCAAACCAGCTTTGGGACCCTGATTCCTTGATTCGGATAGCTCCTGAAGGTGCTAGTGATAGTGATAGTGATAGCAAGTCATATCGATTGAGATCCTATGTGACATACTTGGATGCAGAGACACTTCAGAGATATGCTACTATAAGATCAAAGGAAGCTATTTCTTTGATTGAAAACCAGACACAAGCCCTGTTTGGGAAACCTGACATCAGGATTGCCGCGGATGGTTCAATTGATGCACCTAATGATGAGGTGATTGCACTTACCTTCTCTGGATTGACAATGCTCATCTTGGAAGCTGTTGCTTTCGGATCATTCCTGTGGGATGCTGAAACCTACGTTGAATCTAGATATCACTTTGTCAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF760

Protein Analysis

404

Amino Acids

44.97

Weight (kDa)

5.92

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 142 - 267 6.2e-20 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32160
fragaria_vesca FvH4_5g23190
malus_domestica MD06G1027100.v1.1 MD16G1279100.v1.1
prunus_persica Prupe.5G027900_v2.0.a1 Prupe.5G027900_v2.0.a1
pyrus_communis pycom06g02220
rosa_chinensis RchiOBHm_Chr7g0212111
rosa_laevigata RLG00000002934
rosa_multiflora Rmu_sc0002283.1_g000040
rosa_roxburghii Rroxscaffold_3G00247130
rosa_rugosa Rorug07G0131000
rosa_samantha Rh7AG264000 Rh7BG257700 Rh7CG280500 Rh7DG270500
rosa_wichuraiana Rw7G022530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 259
AccB7I CCANNNNNTGG 1 cut(s) 304
AccBSI CCGCTC 1 cut(s) 778
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 1061
AciI CCGC 4 cut(s) 291, 778, 1059, 1061
AclWI GGATC 5 cut(s) 526, 551, 824, 927, 1159
AcoI YGGCCR 4 cut(s) 65, 94, 295, 581
AcsI RAATTY 3 cut(s) 71, 506, 522
AcuI CTGAAG 3 cut(s) 642, 906, 950
AfaI GTAC 2 cut(s) 660, 749
AgeI ACCGGT 1 cut(s) 563
AgsI TTSAA 9 cut(s) 246, 278, 284, 374, 528, 741, 1013, 1071, 1184
Alw26I GTCTC 3 cut(s) 62, 542, 953
AlwI GGATC 5 cut(s) 526, 551, 824, 927, 1159
AoxI GGCC 6 cut(s) 65, 94, 295, 306, 581, 679
ApaI GGGCCC 1 cut(s) 683
ApeKI GCWGC 4 cut(s) 344, 358, 404, 734
ApoI RAATTY 3 cut(s) 71, 506, 522
ArsI GACNNNNNNTTYG 2 cut(s) 17, 49
AsiGI ACCGGT 1 cut(s) 563
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 4 cut(s) 307, 679, 680, 856
AsuHPI GGTGA 2 cut(s) 677, 1105
AvaII GGWCC 1 cut(s) 856
AxyI CCTNAGG 1 cut(s) 813
BaeGI GKGCMC 1 cut(s) 683
BalI TGGCCA 3 cut(s) 67, 297, 583
BanI GGYRCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 683
BbvI GCAGC 4 cut(s) 356, 370, 391, 721
BccI CCATC 1 cut(s) 1058
BceAI ACGGC 1 cut(s) 109
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 3 cut(s) 62, 542, 953
BfaI CTAG 3 cut(s) 602, 894, 1188
BfmI CTRYAG 2 cut(s) 342, 825
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 5 cut(s) 345, 359, 405, 735, 1059
BlsI GCNGC 5 cut(s) 346, 360, 406, 736, 1060
Bme18I GGWCC 1 cut(s) 856
BmgT120I GGNCC 4 cut(s) 307, 679, 680, 856
BmiI GGNNCC 6 cut(s) 261, 681, 682, 782, 857, 858
BmsI GCATC 6 cut(s) 183, 242, 376, 943, 1066, 1156
BpuEI CTTGAG 2 cut(s) 383, 809
Bsa29I ATCGAT 1 cut(s) 925
BsaBI GATNNNNATC 1 cut(s) 931
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 91, 693, 1059
BsaWI WCCGGW 2 cut(s) 47, 563
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 1 cut(s) 263
Bse21I CCTNAGG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 931
BseCI ATCGAT 1 cut(s) 925
BseDI CCNNGG 3 cut(s) 91, 693, 1059
BseGI GGATG 6 cut(s) 109, 174, 325, 958, 1069, 1171
BseJI GATNNNNATC 1 cut(s) 931
BseMII CTCAG 2 cut(s) 73, 804
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 2 cut(s) 261, 413
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 4 cut(s) 356, 370, 391, 721
BseYI CCCAGC 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 1061
BshFI GGCC 6 cut(s) 67, 96, 297, 308, 583, 681
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 1 cut(s) 563
BshVI ATCGAT 1 cut(s) 925
BsiSI CCGG 2 cut(s) 48, 564
BslFI GGGAC 1 cut(s) 869
BsmAI GTCTC 3 cut(s) 62, 542, 953
BsmFI GGGAC 1 cut(s) 869
BsnI GGCC 6 cut(s) 67, 96, 297, 308, 583, 681
Bso31I GGTCTC 1 cut(s) 62
Bsp120I GGGCCC 1 cut(s) 679
Bsp1286I GDGCHC 1 cut(s) 683
Bsp143I GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 4 cut(s) 291, 778, 1059, 1061
BspANI GGCC 6 cut(s) 67, 96, 297, 308, 583, 681
BspCNI CTCAG 2 cut(s) 72, 805
BspDI ATCGAT 1 cut(s) 925
BspFNI CGCG 1 cut(s) 1061
BspLI GGNNCC 6 cut(s) 261, 681, 682, 782, 857, 858
BspMAI CTGCAG 2 cut(s) 346, 829
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 5 cut(s) 526, 551, 824, 927, 1159
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 778
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 3 cut(s) 91, 693, 1059
BssMI GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
BssT1I CCWWGG 1 cut(s) 693
Bst4CI ACNGT 1 cut(s) 518
Bst6I CTCTTC 2 cut(s) 279, 471
BstC8I GCNNGC 2 cut(s) 295, 299
BstDEI CTNAG 2 cut(s) 59, 813
BstDSI CCRYGG 3 cut(s) 91, 693, 1059
BstENI CCTNNNNNAGG 1 cut(s) 812
BstF5I GGATG 6 cut(s) 109, 174, 325, 958, 1069, 1171
BstFNI CGCG 1 cut(s) 1061
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 7 cut(s) 355, 534, 559, 832, 935, 989, 1154
BstMAI GTCTC 3 cut(s) 62, 542, 953
BstMBI GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
BstMWI GCNNNNNNNGC 3 cut(s) 413, 784, 833
BstSFI CTRYAG 2 cut(s) 342, 825
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 1 cut(s) 1061
BstV1I GCAGC 4 cut(s) 356, 370, 391, 721
BstX2I RGATCY 3 cut(s) 556, 829, 932
BstYI RGATCY 3 cut(s) 556, 829, 932
Bsu15I ATCGAT 1 cut(s) 925
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 6 cut(s) 67, 96, 297, 308, 583, 681
BsuTUI ATCGAT 1 cut(s) 925
BtgI CCRYGG 3 cut(s) 91, 693, 1059
BtsCI GGATG 6 cut(s) 109, 174, 325, 958, 1069, 1171
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 2 cut(s) 117, 822
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 2 cut(s) 295, 299
CfoI GCGC 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 4 cut(s) 307, 679, 680, 856
Cfr42I CCGCGG 1 cut(s) 1062
ClaI ATCGAT 1 cut(s) 925
Csp6I GTAC 2 cut(s) 659, 748
CspAI ACCGGT 1 cut(s) 563
CviAII CATG 4 cut(s) 86, 466, 694, 799
CviQI GTAC 2 cut(s) 659, 748
DdeI CTNAG 2 cut(s) 59, 813
DpnI GATC 7 cut(s) 354, 533, 558, 831, 934, 988, 1153
DpnII GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
EaeI YGGCCR 4 cut(s) 65, 94, 295, 581
Eam1104I CTCTTC 2 cut(s) 279, 471
EarI CTCTTC 2 cut(s) 279, 471
Eco130I CCWWGG 1 cut(s) 693
Eco24I GRGCYC 1 cut(s) 683
Eco31I GGTCTC 1 cut(s) 62
Eco32I GATATC 2 cut(s) 543, 1193
Eco47I GGWCC 1 cut(s) 856
Eco57I CTGAAG 3 cut(s) 642, 906, 950
Eco81I CCTNAGG 1 cut(s) 813
EcoNI CCTNNNNNAGG 1 cut(s) 812
EcoO109I RGGNCCY 2 cut(s) 680, 856
EcoRI GAATTC 1 cut(s) 71
EcoRV GATATC 2 cut(s) 543, 1193
EcoT14I CCWWGG 1 cut(s) 693
EcoT38I GRGCYC 1 cut(s) 683
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 4 cut(s) 89, 469, 697, 802
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FaqI GGGAC 1 cut(s) 869
FatI CATG 4 cut(s) 85, 465, 693, 798
FauI CCCGC 1 cut(s) 298
FauNDI CATATG 1 cut(s) 628
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 5 cut(s) 345, 359, 405, 735, 1059
FokI GGATG 6 cut(s) 96, 161, 312, 965, 1076, 1178
FriOI GRGCYC 1 cut(s) 683
Fsp4HI GCNGC 5 cut(s) 345, 359, 405, 735, 1059
FspBI CTAG 3 cut(s) 602, 894, 1188
GlaI GCGC 1 cut(s) 464
GluI GCNGC 5 cut(s) 345, 359, 405, 735, 1059
GsaI CCCAGC 1 cut(s) 188
HaeIII GGCC 6 cut(s) 67, 96, 297, 308, 583, 681
HapII CCGG 2 cut(s) 48, 564
HhaI GCGC 1 cut(s) 465
Hin1II CATG 4 cut(s) 89, 469, 697, 802
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HinfI GANTC 7 cut(s) 122, 242, 284, 605, 863, 871, 1184
HpaII CCGG 2 cut(s) 48, 564
HphI GGTGA 2 cut(s) 677, 1105
Hpy166II GTNNAC 2 cut(s) 514, 613
Hpy188I TCNGA 7 cut(s) 77, 155, 547, 556, 876, 969, 1151
Hpy188III TCNNGA 7 cut(s) 535, 620, 812, 884, 1051, 1113, 1188
Hpy8I GTNNAC 2 cut(s) 514, 613
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 5 cut(s) 174, 380, 818, 881, 1117
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4IV ACGT 2 cut(s) 27, 1179
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 784, 833
HpyF3I CTNAG 2 cut(s) 59, 813
HpySE526I ACGT 2 cut(s) 27, 1179
Hsp92II CATG 4 cut(s) 89, 469, 697, 802
HspAI GCGC 1 cut(s) 463
KflI GGGWCCC 1 cut(s) 856
Ksp22I TGATCA 1 cut(s) 352
KspI CCGCGG 1 cut(s) 1062
Kzo9I GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
LmnI GCTCC 2 cut(s) 274, 886
Lsp1109I GCAGC 4 cut(s) 356, 370, 391, 721
LweI GCATC 6 cut(s) 183, 242, 376, 943, 1066, 1156
MaeI CTAG 3 cut(s) 602, 894, 1188
MaeII ACGT 2 cut(s) 27, 1179
MaeIII GTNAC 1 cut(s) 940
MalI GATC 7 cut(s) 354, 533, 558, 831, 934, 988, 1153
MbiI CCGCTC 1 cut(s) 778
MboI GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
MboII GAAGA 4 cut(s) 266, 488, 550, 681
MfeI CAATTG 1 cut(s) 1071
MflI RGATCY 3 cut(s) 556, 829, 932
MhlI GDGCHC 1 cut(s) 683
MlsI TGGCCA 3 cut(s) 67, 297, 583
MluCI AATT 8 cut(s) 18, 39, 71, 253, 278, 506, 522, 1071
MluNI TGGCCA 3 cut(s) 67, 297, 583
MlyI GAGTC 1 cut(s) 614
MnlI CCTC 5 cut(s) 55, 282, 434, 808, 1084
Mox20I TGGCCA 3 cut(s) 67, 297, 583
MscI TGGCCA 3 cut(s) 67, 297, 583
MslI CAYNNNNRTG 2 cut(s) 84, 1159
Msp20I TGGCCA 3 cut(s) 67, 297, 583
MspA1I CMGCKG 2 cut(s) 293, 1061
MspI CCGG 2 cut(s) 48, 564
MunI CAATTG 1 cut(s) 1071
MvnI CGCG 1 cut(s) 1061
MwoI GCNNNNNNNGC 3 cut(s) 413, 784, 833
NcoI CCATGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 628
NdeII GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
NlaIII CATG 4 cut(s) 89, 469, 697, 802
NlaIV GGNNCC 6 cut(s) 261, 681, 682, 782, 857, 858
NmeAIII GCCGAG 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 940
PfeI GAWTC 6 cut(s) 122, 242, 284, 863, 871, 1184
PflMI CCANNNNNTGG 1 cut(s) 304
PinAI ACCGGT 1 cut(s) 563
PkrI GCNGC 5 cut(s) 346, 360, 406, 736, 1060
PleI GAGTC 1 cut(s) 613
PpsI GAGTC 1 cut(s) 613
PpuMI RGGWCCY 1 cut(s) 856
Psp5II RGGWCCY 1 cut(s) 856
PspFI CCCAGC 1 cut(s) 184
PspN4I GGNNCC 6 cut(s) 261, 681, 682, 782, 857, 858
PspOMI GGGCCC 1 cut(s) 679
PspPI GGNCC 4 cut(s) 307, 679, 680, 856
PspPPI RGGWCCY 1 cut(s) 856
PstI CTGCAG 2 cut(s) 346, 829
PsuI RGATCY 3 cut(s) 556, 829, 932
RsaI GTAC 2 cut(s) 660, 749
RsaNI GTAC 2 cut(s) 659, 748
RseI CAYNNNNRTG 2 cut(s) 84, 1159
SacII CCGCGG 1 cut(s) 1062
SatI GCNGC 5 cut(s) 345, 359, 405, 735, 1059
Sau3AI GATC 7 cut(s) 352, 531, 556, 829, 932, 986, 1151
Sau96I GGNCC 4 cut(s) 307, 679, 680, 856
SchI GAGTC 1 cut(s) 614
SduI GDGCHC 1 cut(s) 683
SfaNI GCATC 6 cut(s) 183, 242, 376, 943, 1066, 1156
SfcI CTRYAG 2 cut(s) 342, 825
Sfr303I CCGCGG 1 cut(s) 1062
SgrBI CCGCGG 1 cut(s) 1062
SinI GGWCC 1 cut(s) 856
SmiMI CAYNNNNRTG 2 cut(s) 84, 1159
SmlI CTYRAG 2 cut(s) 362, 788
SmoI CTYRAG 2 cut(s) 362, 788
Sse9I AATT 8 cut(s) 18, 39, 71, 253, 278, 506, 522, 1071
SsiI CCGC 4 cut(s) 291, 778, 1059, 1061
SspI AATATT 1 cut(s) 315
SspMI CTAG 3 cut(s) 602, 894, 1188
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 1 cut(s) 518
TaiI ACGT 2 cut(s) 30, 1182
TaqI TCGA 1 cut(s) 925
TasI AATT 8 cut(s) 18, 39, 71, 253, 278, 506, 522, 1071
TatI WGTACW 1 cut(s) 658
TauI GCSGC 1 cut(s) 1061
TfiI GAWTC 6 cut(s) 122, 242, 284, 863, 871, 1184
TscAI CASTG 2 cut(s) 124, 829
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 4 cut(s) 344, 358, 404, 734
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 4 cut(s) 135, 156, 399, 815
TspGWI ACGGA 2 cut(s) 207, 765
TspRI CASTG 2 cut(s) 124, 829
Van91I CCANNNNNTGG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 856
XagI CCTNNNNNAGG 1 cut(s) 812
XapI RAATTY 3 cut(s) 71, 506, 522
XbaI TCTAGA 1 cut(s) 1187
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 3 cut(s) 602, 894, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.