Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010123

Physical Location & Seq

Genomic Coordinates Reverse (-)

3850258 .. 3855305

Gene Structure 5048 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1032100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGGGTTTGGGGATCCTATTTGGCAGTGGGCCAGTGGCCCACAATCCAATGTCCCTACCCACCCATTGTGGTTTGCAAGCCGTCCGCTTATTCCCCTTCTCCCCTATCTCTCTGCTCCAGCAACTCAGACCGCAACCAGACGCCGATCGTCGCCAAATTCCATGTGAAATGGGTTGCACTGTGAGAGAGAAGCACATCCGTACAAATCGAAAGCCCCGATCTACAAAACCCGATGTCGATTATTGTTGTCACTTTGATAGGGCATCAATCTCCAAATCCATACTCGAATCGGGTCTCAAACCGTTCAATCACCATCTGGGTTCCAATGACGATTCCACCCAGAACCCTAAATCGATCCCAGGCCCCAACCCCAATTTGGATGAGAGCGGGTGGGGTTACTGCACTGAGGAACAATTGGAGGAGATTTTATTGAAAAATTTGGAATTTTTGTACAATGAGGCTATTTCTAAGCTAACTGCGTTGGGTTATGATGAGGATGCCGCACTTAAAGCAATATTGCGAAATGGGCATTGCTATGGCGGTATGGATGTGTTAACCAACATTATGCATAACTCATTGGCTTATTTGAATAGTAGTTGTGGAAGCAGTAATGGGAAGTCGGATGAGGCTGAGCCTGTTTTTGCTGATTTAAGGCAGCTGGAGGAGTACTCGCTTGCGGGTATGGTGTGTATGTTGCAACAAGTTAGGCCACATTTAAGTAAAGGTGATGCTATGTGGTGTTTGCTTATGAGTGATCTTCATGTGGGTCGGGCAAGTACTATGGAAATTCCCTCCCTTCCCAGTGGAGGTAATGAAAATAATAGCTCGACATCCAGTAATGTTGAATCTGGTAGTAATGGGAACCATTCAGTTGGCGTTATGGCTCCTGCACTTTGTAGGTTTCATGGTGGTTGGGGGTTTGGAGATGGGGGAGCTTCAGAGTTTCCAGTTCCTGGGTTTTTCTCTTATGGTGCTGAATTGACTTTGCAAAGGGATATTGAGTGTCCCAAGAGGTTTAACCTTTCTCCCTCCATGAAGTCGTTGCTGAAAAGGAATGTTGCAATGTTTGCAGCTGGTTTTCGAGCAAACTCAAAACAGTTAAATGCTCAACCACAGGCTTGCCTGAACAGTTTAACTAGTAGGGACACCTCGGTTGGACTGGGGTCTGAAGCTGCAGTTCATCAGTCTGATGAGTCAAAGAATTCAAAGAACCAGGAGGTGGTTAACTCAGTGTTGAGTAAGTTTCGCGATTTGAATCTTGATGAGAATATGGAGTTTGTGGGGGAAGATCAAAAGAATGAGGTGATAGTTACTCTACTTCATCAAGTAAAAGAGCTTGAGAAGCAAGTTAAGGAACGGAAGGAATGGGCGCACCAGAAGGCAATGCAGGCTGCAACAAAGCTCAGTCACGATCTTGCAGAGCTTAAAATGCTGAGGCTGGAAAGGGAGGAGACTCAAAGGTTGAAGAAAGGGAAGCAAACTCTTGAGGACTCAACCATGAAGAGACTCTCAGAAATGGAAAACGCTCTAAGAAAGGCCAGTGGTCAAGTGGACCGCGCAAATGCAGCTGTGAGACGGCTTGAGACTGAGAATGCAGAAATCAGAGCAGAGATGGAGGCTTCTAAACTGAGTGCATCAGAGTCAGTAACAACTTGTTTGGAGGTTGCAAAGAGGGAGAAAAAATATCTGAAGAGGCTTTTGGCTTGGGAGAAACAGAAAACTAAATTGCAGGAAGAGATTGCAGAGGAGAAACAGAAGATTTCAGAATTGCAACAACATTTGGCTCGGATGAAGCATGATCAGAAAGAAGCTGAGGTGAAATGGAGGCAGGAACAGAAGGCTAAGGAGGAAGCCTTGACTCAAGTGGAAGAGGAACGACGTGCTAAGGAAGCAGCTGAGGCTAGTAATAAAAGAAAGCTTGAAGCTTTGCGCCTAAAGATAGAAATAGATTTCCAGCGTCACAAGGATGACCTCCAAAGACTTGAGCAGGAACTCTCACGTCTAAAAGTATCCGCCCAGTCTACTGAATCGGTTCATCCATCAAATGCGTTGCCAAAAGCAATCCCAGAAGGTGCAAAACCCCCAGGAGAAACTATTGCTAAGATGCTTCGTGATTTGGATAACCTGGAGAAATCAGCAGAGAAAGAAGTGGGCTGTGATAGGGAATGCATAATTTGCATGAGAGATGAAGTCTCTGTTGTTTTCCTGCCATGTGCTCATCAAGTTCTTTGTGCCAATTGCAATGATGATTACGGGAAAAAAGGTAAGGCCACCTGCCCATGTTGCCAGGCTCCTATTGAACATAGAATTCGTGTTTTTGGTGCAAGTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009987 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

778

Amino Acids

86.69

Weight (kDa)

6.55

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIR2-like_helical	PF20235	146 - 261	1.6e-27	PIR2-like, helical domain
zf-C3HC4_3	PF13920	720 - 768	2.7e-11	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010123)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35330 AT2G35330
fragaria_vesca	FvH4_5g21710 FvH4_5g21710
malus_domestica	MD06G1032100.v1.1
prunus_persica	Prupe.5G040000_v2.0.a1 Prupe.5G040000_v2.0.a1
pyrus_communis	pycom06g02660
rosa_chinensis	RchiOBHm_Chr7g0209211
rosa_laevigata	RLG00000003157
rosa_multiflora	Rmu_sc0001701.1_g000007 Rmu_sc0031054.1_g000002
rosa_roxburghii	Rroxscaffold_3G00249630
rosa_rugosa	Rorug07G0110200 Rorug07G0110300 Rorug07G0110400
rosa_samantha	Rh7AG244300 Rh7BG239500 Rh7CG259800 Rh7DG251100
rosa_wichuraiana	Rw7G020720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2282
Acc36I	ACCTGC	1 cut(s)	2282
AccB7I	CCANNNNNTGG	2 cut(s)	953, 1219
AccBSI	CCGCTC	1 cut(s)	387
AccI	GTMKAC	1 cut(s)	2021
AccII	CGCG	2 cut(s)	1248, 1557
AciI	CCGC	8 cut(s)	85, 131, 387, 501, 540, 677, 1555, 2013
AclWI	GGATC	3 cut(s)	7, 20, 349
AcsI	RAATTY	6 cut(s)	156, 436, 443, 786, 1201, 2307
AcuI	CTGAAG	3 cut(s)	921, 1188, 1709
AcyI	GRCGYC	1 cut(s)	141
AfaI	GTAC	4 cut(s)	202, 452, 668, 778
AfiI	CCNNNNNNNGG	4 cut(s)	376, 806, 953, 1219
AgsI	TTSAA	9 cut(s)	307, 433, 589, 845, 1206, 1255, 1465, 1922, 2300
AhlI	ACTAGT	1 cut(s)	1136
AjiI	CACGTC	2 cut(s)	1880, 2000
AjnI	CCWGG	6 cut(s)	358, 952, 1212, 2083, 2124, 2286
AjuI	GAANNNNNNNTTGG	2 cut(s)	1682, 1714
Alw21I	GWGCWC	1 cut(s)	2218
Alw26I	GTCTC	6 cut(s)	299, 1445, 1498, 1567, 1577, 2197
AlwI	GGATC	3 cut(s)	7, 20, 349
AlwNI	CAGNNNCTG	1 cut(s)	953
AoxI	GGCC	6 cut(s)	29, 36, 361, 707, 1536, 2268
ApeKI	GCWGC	6 cut(s)	655, 1070, 1172, 1391, 1565, 1892
ApoI	RAATTY	6 cut(s)	156, 436, 443, 786, 1201, 2307
Asp700I	GAANNNNTTC	1 cut(s)	2031
AspLEI	GCGC	3 cut(s)	1372, 1559, 1932
AspS9I	GGNCC	4 cut(s)	29, 37, 362, 1552
AsuHPI	GGTGA	4 cut(s)	302, 737, 1315, 1828
AvaII	GGWCC	1 cut(s)	1552
BamHI	GGATCC	1 cut(s)	12
Bbv12I	GWGCWC	1 cut(s)	2218
BbvCI	CCTCAGC	3 cut(s)	1433, 1812, 1896
BbvI	GCAGC	6 cut(s)	667, 1082, 1159, 1378, 1577, 1904
BccI	CCATC	4 cut(s)	321, 920, 1606, 2047
BceAI	ACGGC	2 cut(s)	65, 1592
BciT130I	CCWGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
BciVI	GTATCC	1 cut(s)	2020
BclI	TGATCA	1 cut(s)	1798
BcoDI	GTCTC	6 cut(s)	299, 1445, 1498, 1567, 1577, 2197
BcuI	ACTAGT	1 cut(s)	1136
BfaI	CTAG	2 cut(s)	1137, 1902
BfmI	CTRYAG	1 cut(s)	1173
BfuAI	ACCTGC	1 cut(s)	2282
BfuI	GTATCC	1 cut(s)	2020
BisI	GCNGC	7 cut(s)	501, 656, 1071, 1173, 1392, 1566, 1893
BlpI	GCTNAGC	1 cut(s)	630
BlsI	GCNGC	7 cut(s)	502, 657, 1072, 1174, 1393, 1567, 1894
BmcAI	AGTACT	2 cut(s)	668, 778
Bme1390I	CCNGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
Bme18I	GGWCC	1 cut(s)	1552
BmgBI	CACGTC	2 cut(s)	1880, 2000
BmgT120I	GGNCC	4 cut(s)	29, 37, 362, 1552
BmiI	GGNNCC	6 cut(s)	14, 322, 364, 863, 885, 2292
BmrFI	CCNGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
BmrI	ACTGGG	3 cut(s)	795, 1169, 2011
BmsI	GCATC	5 cut(s)	272, 487, 718, 1643, 2094
BmuI	ACTGGG	3 cut(s)	795, 1169, 2011
BoxI	GACNNNNGTC	1 cut(s)	1162
BplI	GAGNNNNNCTC	2 cut(s)	653, 685
BpmI	CTGGAG	3 cut(s)	101, 680, 2147
Bpu10I	CCTNAGC	5 cut(s)	1433, 1812, 1842, 1884, 1896
Bpu1102I	GCTNAGC	1 cut(s)	630
BpuEI	CTTGAG	5 cut(s)	1358, 1505, 1601, 1845, 2003
Bsa29I	ATCGAT	1 cut(s)	353
BsaBI	GATNNNNATC	1 cut(s)	1254
BsaHI	GRCGYC	1 cut(s)	141
BsaI	GGTCTC	1 cut(s)	299
BsaJI	CCNNGG	4 cut(s)	358, 953, 1149, 2083
Bsc4I	CCNNNNNNNGG	4 cut(s)	376, 806, 953, 1219
Bse1I	ACTGG	7 cut(s)	32, 801, 834, 947, 1164, 1539, 2017
Bse3DI	GCAATG	4 cut(s)	529, 1068, 1389, 2248
Bse8I	GATNNNNATC	1 cut(s)	1254
BseBI	CCWGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
BseCI	ATCGAT	1 cut(s)	353
BseDI	CCNNGG	4 cut(s)	358, 953, 1149, 2083
BseGI	GGATG	9 cut(s)	195, 385, 502, 553, 628, 830, 1794, 1972, 2035
BseJI	GATNNNNATC	1 cut(s)	1254
BseLI	CCNNNNNNNGG	4 cut(s)	376, 806, 953, 1219
BseMI	GCAATG	4 cut(s)	529, 1068, 1389, 2248
BseNI	ACTGG	7 cut(s)	32, 801, 834, 947, 1164, 1539, 2017
BseRI	GAGGAG	4 cut(s)	434, 677, 1463, 1760
BseXI	GCAGC	6 cut(s)	667, 1082, 1159, 1378, 1577, 1904
BsgI	GTGCAG	2 cut(s)	385, 873
Bsh1236I	CGCG	2 cut(s)	1248, 1557
Bsh1285I	CGRYCG	1 cut(s)	148
BshFI	GGCC	6 cut(s)	31, 38, 363, 709, 1538, 2270
BshVI	ATCGAT	1 cut(s)	353
BsiEI	CGRYCG	1 cut(s)	148
BsiHKAI	GWGCWC	1 cut(s)	2218
BslFI	GGGAC	3 cut(s)	37, 990, 1157
BslI	CCNNNNNNNGG	4 cut(s)	376, 806, 953, 1219
BsmAI	GTCTC	6 cut(s)	299, 1445, 1498, 1567, 1577, 2197
BsmBI	CGTCTC	1 cut(s)	1567
BsmFI	GGGAC	3 cut(s)	37, 990, 1157
BsmI	GAATGC	2 cut(s)	1597, 2171
BsnI	GGCC	6 cut(s)	31, 38, 363, 709, 1538, 2270
Bso31I	GGTCTC	1 cut(s)	299
Bsp1286I	GDGCHC	1 cut(s)	2218
Bsp1407I	TGTACA	1 cut(s)	450
Bsp143I	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
Bsp1720I	GCTNAGC	1 cut(s)	630
Bsp68I	TCGCGA	1 cut(s)	1248
BspACI	CCGC	8 cut(s)	85, 131, 387, 501, 540, 677, 1555, 2013
BspANI	GGCC	6 cut(s)	31, 38, 363, 709, 1538, 2270
BspDI	ATCGAT	1 cut(s)	353
BspFNI	CGCG	2 cut(s)	1248, 1557
BspLI	GGNNCC	6 cut(s)	14, 322, 364, 863, 885, 2292
BspMAI	CTGCAG	1 cut(s)	1177
BspMI	ACCTGC	1 cut(s)	2282
BspPI	GGATC	3 cut(s)	7, 20, 349
BspTNI	GGTCTC	1 cut(s)	299
BsrBI	CCGCTC	1 cut(s)	387
BsrDI	GCAATG	4 cut(s)	529, 1068, 1389, 2248
BsrGI	TGTACA	1 cut(s)	450
BsrI	ACTGG	7 cut(s)	32, 801, 834, 947, 1164, 1539, 2017
BssECI	CCNNGG	4 cut(s)	358, 953, 1149, 2083
BssMI	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
BssNI	GRCGYC	1 cut(s)	141
Bst2UI	CCWGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
Bst4CI	ACNGT	4 cut(s)	181, 303, 1098, 1130
Bst6I	CTCTTC	4 cut(s)	1496, 1685, 1728, 1863
BstACI	GRCGYC	1 cut(s)	141
BstAPI	GCANNNNNTGC	2 cut(s)	1067, 2175
BstAUI	TGTACA	1 cut(s)	450
BstC8I	GCNNGC	4 cut(s)	78, 675, 1120, 1389
BstF5I	GGATG	9 cut(s)	195, 385, 502, 553, 628, 830, 1794, 1972, 2035
BstFNI	CGCG	2 cut(s)	1248, 1557
BstHHI	GCGC	3 cut(s)	1372, 1559, 1932
BstKTI	GATC	8 cut(s)	15, 148, 221, 357, 757, 1291, 1414, 1801
BstMAI	GTCTC	6 cut(s)	299, 1445, 1498, 1567, 1577, 2197
BstMBI	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
BstMCI	CGRYCG	1 cut(s)	148
BstNI	CCWGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
BstPAI	GACNNNNGTC	1 cut(s)	1162
BstSCI	CCNGG	6 cut(s)	358, 952, 1212, 2083, 2124, 2286
BstSFI	CTRYAG	1 cut(s)	1173
BstUI	CGCG	2 cut(s)	1248, 1557
BstV1I	GCAGC	6 cut(s)	667, 1082, 1159, 1378, 1577, 1904
BstX2I	RGATCY	1 cut(s)	12
BstXI	CCANNNNNNTGG	1 cut(s)	872
BstYI	RGATCY	1 cut(s)	12
Bsu15I	ATCGAT	1 cut(s)	353
BsuI	GTATCC	1 cut(s)	2020
BsuRI	GGCC	6 cut(s)	31, 38, 363, 709, 1538, 2270
BsuTUI	ATCGAT	1 cut(s)	353
BtrI	CACGTC	2 cut(s)	1880, 2000
BtsCI	GGATG	9 cut(s)	195, 385, 502, 553, 628, 830, 1794, 1972, 2035
BtsI	GCAGTG	1 cut(s)	31
BtsIMutI	CAGTG	7 cut(s)	31, 39, 177, 402, 808, 1236, 1546
BtuMI	TCGCGA	1 cut(s)	1248
BveI	ACCTGC	1 cut(s)	2282
Cac8I	GCNNGC	4 cut(s)	78, 675, 1120, 1389
CaiI	CAGNNNCTG	1 cut(s)	953
CfoI	GCGC	3 cut(s)	1372, 1559, 1932
Cfr13I	GGNCC	4 cut(s)	29, 37, 362, 1552
ClaI	ATCGAT	1 cut(s)	353
CseI	GACGC	2 cut(s)	149, 1946
Csp6I	GTAC	4 cut(s)	201, 451, 667, 777
CviAII	CATG	9 cut(s)	162, 761, 905, 1033, 1498, 1796, 2179, 2211, 2280
CviQI	GTAC	4 cut(s)	201, 451, 667, 777
DpnI	GATC	8 cut(s)	14, 147, 220, 356, 756, 1290, 1413, 1800
DpnII	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
Eam1104I	CTCTTC	4 cut(s)	1496, 1685, 1728, 1863
EarI	CTCTTC	4 cut(s)	1496, 1685, 1728, 1863
EciI	GGCGGA	1 cut(s)	2002
Eco31I	GGTCTC	1 cut(s)	299
Eco47I	GGWCC	1 cut(s)	1552
Eco57I	CTGAAG	3 cut(s)	921, 1188, 1709
EcoO109I	RGGNCCY	1 cut(s)	362
EcoRI	GAATTC	2 cut(s)	1201, 2307
EcoRII	CCWGG	6 cut(s)	358, 952, 1212, 2083, 2124, 2286
EcoT22I	ATGCAT	2 cut(s)	570, 2171
Esp3I	CGTCTC	1 cut(s)	1567
FaeI	CATG	9 cut(s)	165, 764, 908, 1036, 1501, 1799, 2182, 2214, 2283
FaqI	GGGAC	3 cut(s)	37, 990, 1157
FatI	CATG	9 cut(s)	161, 760, 904, 1032, 1497, 1795, 2178, 2210, 2279
FauI	CCCGC	2 cut(s)	380, 670
FbaI	TGATCA	1 cut(s)	1798
FblI	GTMKAC	1 cut(s)	2021
Fnu4HI	GCNGC	7 cut(s)	501, 656, 1071, 1173, 1392, 1566, 1893
FokI	GGATG	9 cut(s)	182, 392, 509, 560, 635, 817, 1801, 1979, 2022
Fsp4HI	GCNGC	7 cut(s)	501, 656, 1071, 1173, 1392, 1566, 1893
FspBI	CTAG	2 cut(s)	1137, 1902
GlaI	GCGC	3 cut(s)	1371, 1558, 1931
GluI	GCNGC	7 cut(s)	501, 656, 1071, 1173, 1392, 1566, 1893
GsuI	CTGGAG	3 cut(s)	101, 680, 2147
HaeIII	GGCC	6 cut(s)	31, 38, 363, 709, 1538, 2270
HgaI	GACGC	2 cut(s)	149, 1946
HhaI	GCGC	3 cut(s)	1372, 1559, 1932
Hin1I	GRCGYC	1 cut(s)	141
Hin1II	CATG	9 cut(s)	165, 764, 908, 1036, 1501, 1799, 2182, 2214, 2283
Hin6I	GCGC	3 cut(s)	1370, 1557, 1930
HinP1I	GCGC	3 cut(s)	1370, 1557, 1930
HincII	GTYRAC	2 cut(s)	555, 1225
HindII	GTYRAC	2 cut(s)	555, 1225
HindIII	AAGCTT	2 cut(s)	1916, 1923
HpaI	GTTAAC	2 cut(s)	555, 1225
HphI	GGTGA	4 cut(s)	302, 737, 1315, 1828
Hpy166II	GTNNAC	4 cut(s)	555, 1225, 1552, 2022
Hpy188III	TCNNGA	5 cut(s)	1247, 1259, 1409, 1484, 2111
Hpy8I	GTNNAC	4 cut(s)	555, 1225, 1552, 2022
Hpy99I	CGWCG	2 cut(s)	153, 1881
HpyAV	CCTTC	6 cut(s)	106, 806, 1354, 1372, 1831, 2063
HpyCH4III	ACNGT	4 cut(s)	181, 303, 1098, 1130
HpyCH4IV	ACGT	2 cut(s)	1879, 1999
HpySE526I	ACGT	2 cut(s)	1879, 1999
Hsp92I	GRCGYC	1 cut(s)	141
Hsp92II	CATG	9 cut(s)	165, 764, 908, 1036, 1501, 1799, 2182, 2214, 2283
HspAI	GCGC	3 cut(s)	1370, 1557, 1930
Ksp22I	TGATCA	1 cut(s)	1798
KspAI	GTTAAC	2 cut(s)	555, 1225
Kzo9I	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
LmnI	GCTCC	4 cut(s)	120, 889, 932, 2296
Lsp1109I	GCAGC	6 cut(s)	667, 1082, 1159, 1378, 1577, 1904
LweI	GCATC	5 cut(s)	272, 487, 718, 1643, 2094
MaeI	CTAG	2 cut(s)	1137, 1902
MaeII	ACGT	2 cut(s)	1879, 1999
MaeIII	GTNAC	6 cut(s)	248, 395, 1309, 1406, 1645, 1958
MalI	GATC	8 cut(s)	14, 147, 220, 356, 756, 1290, 1413, 1800
MbiI	CCGCTC	1 cut(s)	387
MboI	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
MboII	GAAGA	8 cut(s)	749, 1298, 1477, 1513, 1702, 1745, 1768, 1880
MfeI	CAATTG	2 cut(s)	413, 2236
MflI	RGATCY	1 cut(s)	12
MhlI	GDGCHC	1 cut(s)	2218
MlyI	GAGTC	6 cut(s)	1202, 1447, 1484, 1500, 1649, 1852
MmeI	TCCRAC	2 cut(s)	600, 1135
Mph1103I	ATGCAT	2 cut(s)	570, 2171
MroXI	GAANNNNTTC	1 cut(s)	2031
MslI	CAYNNNNRTG	2 cut(s)	534, 1965
MspA1I	CMGCKG	4 cut(s)	658, 1073, 1568, 1895
MspR9I	CCNGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
MunI	CAATTG	2 cut(s)	413, 2236
Mva1269I	GAATGC	2 cut(s)	1597, 2171
MvaI	CCWGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
MvnI	CGCG	2 cut(s)	1248, 1557
NdeII	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
NlaIII	CATG	9 cut(s)	165, 764, 908, 1036, 1501, 1799, 2182, 2214, 2283
NlaIV	GGNNCC	6 cut(s)	14, 322, 364, 863, 885, 2292
NmuCI	GTSAC	3 cut(s)	248, 1406, 1958
NruI	TCGCGA	1 cut(s)	1248
NsiI	ATGCAT	2 cut(s)	570, 2171
PaqCI	CACCTGC	1 cut(s)	2282
PcsI	WCGNNNNNNNCGW	1 cut(s)	214
PctI	GAATGC	2 cut(s)	1597, 2171
PdmI	GAANNNNTTC	1 cut(s)	2031
PfeI	GAWTC	5 cut(s)	287, 332, 845, 1255, 2027
PflMI	CCANNNNNTGG	2 cut(s)	953, 1219
PkrI	GCNGC	7 cut(s)	502, 657, 1072, 1174, 1393, 1567, 1894
Ple19I	CGATCG	1 cut(s)	148
PleI	GAGTC	6 cut(s)	1201, 1447, 1484, 1500, 1648, 1852
PpsI	GAGTC	6 cut(s)	1201, 1447, 1484, 1500, 1648, 1852
PshAI	GACNNNNGTC	1 cut(s)	1162
Psp6I	CCWGG	6 cut(s)	358, 952, 1212, 2083, 2124, 2286
PspGI	CCWGG	6 cut(s)	358, 952, 1212, 2083, 2124, 2286
PspN4I	GGNNCC	6 cut(s)	14, 322, 364, 863, 885, 2292
PspPI	GGNCC	4 cut(s)	29, 37, 362, 1552
PstI	CTGCAG	1 cut(s)	1177
PstNI	CAGNNNCTG	1 cut(s)	953
PsuI	RGATCY	1 cut(s)	12
PvuI	CGATCG	1 cut(s)	148
PvuII	CAGCTG	4 cut(s)	658, 1073, 1568, 1895
RruI	TCGCGA	1 cut(s)	1248
RsaI	GTAC	4 cut(s)	202, 452, 668, 778
RsaNI	GTAC	4 cut(s)	201, 451, 667, 777
RseI	CAYNNNNRTG	2 cut(s)	534, 1965
SatI	GCNGC	7 cut(s)	501, 656, 1071, 1173, 1392, 1566, 1893
Sau3AI	GATC	8 cut(s)	12, 145, 218, 354, 754, 1288, 1411, 1798
Sau96I	GGNCC	4 cut(s)	29, 37, 362, 1552
ScaI	AGTACT	2 cut(s)	668, 778
SchI	GAGTC	6 cut(s)	1202, 1447, 1484, 1500, 1649, 1852
ScrFI	CCNGG	6 cut(s)	360, 954, 1214, 2085, 2126, 2288
SduI	GDGCHC	1 cut(s)	2218
SfaNI	GCATC	5 cut(s)	272, 487, 718, 1643, 2094
SfcI	CTRYAG	1 cut(s)	1173
SinI	GGWCC	1 cut(s)	1552
SmiMI	CAYNNNNRTG	2 cut(s)	534, 1965
SmlI	CTYRAG	5 cut(s)	1337, 1484, 1580, 1860, 1982
SmoI	CTYRAG	5 cut(s)	1337, 1484, 1580, 1860, 1982
SpeI	ACTAGT	1 cut(s)	1136
SsiI	CCGC	8 cut(s)	85, 131, 387, 501, 540, 677, 1555, 2013
SspI	AATATT	1 cut(s)	516
SspMI	CTAG	2 cut(s)	1137, 1902
StyD4I	CCNGG	6 cut(s)	358, 952, 1212, 2083, 2124, 2286
TaaI	ACNGT	4 cut(s)	181, 303, 1098, 1130
TaiI	ACGT	2 cut(s)	1882, 2002
TaqI	TCGA	6 cut(s)	208, 237, 285, 353, 827, 1081
TatI	WGTACW	3 cut(s)	450, 666, 776
TauI	GCSGC	1 cut(s)	503
TfiI	GAWTC	5 cut(s)	287, 332, 845, 1255, 2027
TscAI	CASTG	7 cut(s)	31, 39, 184, 409, 808, 1236, 1546
TseFI	GTSAC	3 cut(s)	248, 1406, 1958
TseI	GCWGC	6 cut(s)	655, 1070, 1172, 1391, 1565, 1892
Tsp45I	GTSAC	3 cut(s)	248, 1406, 1958
TspGWI	ACGGA	2 cut(s)	188, 1372
TspRI	CASTG	7 cut(s)	31, 39, 184, 409, 808, 1236, 1546
Van91I	CCANNNNNTGG	2 cut(s)	953, 1219
VpaK11BI	GGWCC	1 cut(s)	1552
XapI	RAATTY	6 cut(s)	156, 436, 443, 786, 1201, 2307
XcmI	CCANNNNNNNNNTGG	2 cut(s)	373, 1546
XmiI	GTMKAC	1 cut(s)	2021
XmnI	GAANNNNTTC	1 cut(s)	2031
XspI	CTAG	2 cut(s)	1137, 1902
ZrmI	AGTACT	2 cut(s)	668, 778
Zsp2I	ATGCAT	2 cut(s)	570, 2171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.