Rorug07G0110300

MND1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010123
Physical Location & Seq
Reverse (-)
8590961 .. 8592016
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0110300.1

Sequence Viewer

Length: 1056 bp
ATGGACCGAGAGTGGGGTTCAAAGCCCGGAGGTGGAGGCGCCGCCTCCGCCCAGAACGAAGCCATCGACCGCCGCGAGCGACTCCGACGACTTGCCCTCGAGACCATCGATCTCGCCAAAGATCCATACTTCATGCGCAATCACCTCGGAAGCTATGAGTGTAAACTTTGCTTGACCTTGCACAACAATGAGGGTAACTACTTGGCCTATACACAGGGAAAGCGCCACCAGACCTATCTCGCCAAGCGGGCCACGCGTGAGGCCAAGGATATTCCTGCTCAGCCCCAGCCCCACAAGCGCAAAGTCTCCGTTCGCAAAACAGTGAAAATTGGTAGGCCGGGCTATAGAGTAACAAAGCAGTTTGATGGAGAGACAAAGCAAAGGTCCCTTCTCTTCCAGATTGAGTATCCGGAAATAGAAGATAATGCAAAGCCAAGGCATCGTTTTATGTCATCTTATGAGCAGAGGGTCCAACCCTATGAGAAAAGATACCAGTATCTTCTGTTTGCAGCCGAACCATACGAGATAATTGCTTTTAAGGTTCCTAGTACAGAGATTGACAAATCGACTCCGAAGTTCTTCTCACATTGGGATCCAAACTCCAAAATGTTCACGTTGCAGCTATATTTCAAAGTTAAGCCACCAGAGACGAACAAACCTCAACCTCCCCCAAATGCCAATGGCACAGGTGCTCCTGGAGTTCCTTCAAGGCCTCTACCCCCACCACCCCGAGGTCCACCTCCTCCTCCTCCACAAGGAATGGAGCCTGGTGCACCTATGGGAAACCCTCCTAGAGCTCCACCAACTATTCCTGGTTCCTTACTGCCACCTCCTCCTATGGTAAATGGCCCTGGGCCTATGCCTCCTGGTGGGGCTCCACCTGCCCCACCTCCACCTCCTGTCAGCAATAACACAATGGTGAATTTCACTCCTGGTACCCAGGTGGGTAGACCACCGACTATGCCACCTCCACCACAAGCTTTCCAAGGACAACAGATGCAGGGCCAGGGTCTTCGTCCTCCTCCTCCTCCTACACCTCCTAGCATGGGTCGTTAA

Protein Analysis

351

Amino Acids

38.87

Weight (kDa)

9.95

Isoelectric Point (pI)

60.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SF3A2 PF16835 110 - 204 1.2e-36 Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 889
Acc16I TGCGCA 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 889
Acc65I GGTACC 1 cut(s) 935
AccB1I GGYRCC 2 cut(s) 38, 935
AccI GTMKAC 1 cut(s) 949
AccII CGCG 2 cut(s) 75, 256
AccIII TCCGGA 1 cut(s) 409
AciI CCGC 5 cut(s) 42, 48, 70, 73, 247
AclWI GGATC 3 cut(s) 116, 587, 600
AcsI RAATTY 1 cut(s) 922
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 550, 937
AfiI CCNNNNNNNGG 6 cut(s) 13, 32, 731, 755, 869, 1046
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 3 cut(s) 21, 631, 708
AjnI CCWGG 8 cut(s) 694, 766, 811, 850, 865, 931, 939, 1005
AleI CACNNNNGTG 1 cut(s) 917
AluBI AGCT 4 cut(s) 153, 622, 797, 980
AluI AGCT 4 cut(s) 153, 622, 797, 980
Alw21I GWGCWC 3 cut(s) 694, 775, 799
Alw26I GTCTC 4 cut(s) 95, 310, 365, 641
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 3 cut(s) 116, 587, 600
Ama87I CYCGRG 2 cut(s) 98, 729
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 8 cut(s) 204, 249, 261, 335, 710, 847, 854, 1003
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 2 cut(s) 509, 619
ApoI RAATTY 1 cut(s) 922
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
Asp700I GAANNNNTTC 1 cut(s) 578
Asp718I GGTACC 1 cut(s) 935
AspLEI GCGC 4 cut(s) 41, 138, 225, 300
AspS9I GGNCC 8 cut(s) 4, 249, 384, 469, 734, 848, 854, 1003
AsuC2I CCSGG 2 cut(s) 27, 339
AsuHPI GGTGA 2 cut(s) 134, 931
AvaI CYCGRG 2 cut(s) 98, 729
AvaII GGWCC 4 cut(s) 4, 384, 469, 734
BaeGI GKGCMC 1 cut(s) 775
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 2 cut(s) 38, 935
BanII GRGCYC 2 cut(s) 799, 877
BbsI GAAGAC 1 cut(s) 1004
Bbv12I GWGCWC 3 cut(s) 694, 775, 799
BbvI GCAGC 2 cut(s) 521, 631
BccI CCATC 3 cut(s) 71, 113, 359
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 512, 546
BciT130I CCWGG 8 cut(s) 696, 768, 813, 852, 867, 933, 941, 1007
BciVI GTATCC 1 cut(s) 417
BcnI CCSGG 2 cut(s) 27, 339
BcoDI GTCTC 4 cut(s) 95, 310, 365, 641
BfaI CTAG 3 cut(s) 546, 792, 1041
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 2 cut(s) 42, 226
BfuAI ACCTGC 1 cut(s) 889
BfuI GTATCC 1 cut(s) 417
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 4 cut(s) 42, 73, 510, 620
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 4 cut(s) 43, 74, 511, 621
Bme18I GGWCC 4 cut(s) 4, 384, 469, 734
BmeT110I CYCGRG 2 cut(s) 98, 729
BmgT120I GGNCC 8 cut(s) 4, 249, 384, 469, 734, 848, 854, 1003
BmiI GGNNCC 9 cut(s) 40, 386, 470, 543, 594, 765, 817, 876, 937
BmsI GCATC 2 cut(s) 448, 987
BpiI GAAGAC 1 cut(s) 1004
BpmI CTGGAG 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 27, 339
Bsa29I ATCGAT 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 9 cut(s) 145, 264, 434, 730, 850, 851, 939, 985, 1006
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 2 cut(s) 676, 706
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 32, 731, 755, 869, 1046
Bse1I ACTGG 1 cut(s) 493
BseAI TCCGGA 1 cut(s) 409
BseBI CCWGG 8 cut(s) 696, 768, 813, 852, 867, 933, 941, 1007
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 9 cut(s) 145, 264, 434, 730, 850, 851, 939, 985, 1006
BseLI CCNNNNNNNGG 6 cut(s) 13, 32, 731, 755, 869, 1046
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 493
BseRI GAGGAG 7 cut(s) 732, 735, 738, 822, 1011, 1014, 1017
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 2 cut(s) 521, 631
BseYI CCCAGC 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 75, 256
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 8 cut(s) 206, 251, 263, 337, 712, 849, 856, 1005
BshNI GGYRCC 2 cut(s) 38, 935
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 3 cut(s) 694, 775, 799
BsiHKCI CYCGRG 2 cut(s) 98, 729
BsiSI CCGG 3 cut(s) 27, 338, 410
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 6 cut(s) 13, 32, 731, 755, 869, 1046
BsmAI GTCTC 4 cut(s) 95, 310, 365, 641
BsmBI CGTCTC 1 cut(s) 641
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 8 cut(s) 206, 251, 263, 337, 712, 849, 856, 1005
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 2 cut(s) 98, 729
Bsp1286I GDGCHC 4 cut(s) 694, 775, 799, 877
Bsp13I TCCGGA 1 cut(s) 409
Bsp143I GATC 3 cut(s) 109, 121, 592
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 5 cut(s) 42, 48, 70, 73, 247
BspANI GGCC 8 cut(s) 206, 251, 263, 337, 712, 849, 856, 1005
BspCNI CTCAG 1 cut(s) 292
BspDI ATCGAT 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 409
BspFNI CGCG 2 cut(s) 75, 256
BspLI GGNNCC 9 cut(s) 40, 386, 470, 543, 594, 765, 817, 876, 937
BspMI ACCTGC 1 cut(s) 889
BspPI GGATC 3 cut(s) 116, 587, 600
BspT107I GGYRCC 2 cut(s) 38, 935
BspTNI GGTCTC 1 cut(s) 95
BsrI ACTGG 1 cut(s) 493
BssECI CCNNGG 9 cut(s) 145, 264, 434, 730, 850, 851, 939, 985, 1006
BssMI GATC 3 cut(s) 109, 121, 592
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 3 cut(s) 264, 434, 985
Bst2UI CCWGG 8 cut(s) 696, 768, 813, 852, 867, 933, 941, 1007
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 77, 249
BstDEI CTNAG 1 cut(s) 279
BstENI CCTNNNNNAGG 1 cut(s) 753
BstFNI CGCG 2 cut(s) 75, 256
BstH2I RGCGCY 2 cut(s) 42, 226
BstHHI GCGC 4 cut(s) 41, 138, 225, 300
BstKTI GATC 3 cut(s) 112, 124, 595
BstMAI GTCTC 4 cut(s) 95, 310, 365, 641
BstMBI GATC 3 cut(s) 109, 121, 592
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 5 cut(s) 47, 248, 253, 295, 881
BstNI CCWGG 8 cut(s) 696, 768, 813, 852, 867, 933, 941, 1007
BstSFI CTRYAG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 2 cut(s) 75, 256
BstV1I GCAGC 2 cut(s) 521, 631
BstV2I GAAGAC 1 cut(s) 1004
BstX2I RGATCY 2 cut(s) 121, 592
BstYI RGATCY 2 cut(s) 121, 592
Bsu15I ATCGAT 1 cut(s) 108
BsuI GTATCC 1 cut(s) 417
BsuRI GGCC 8 cut(s) 206, 251, 263, 337, 712, 849, 856, 1005
BsuTUI ATCGAT 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 327
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 2 cut(s) 77, 249
CfoI GCGC 4 cut(s) 41, 138, 225, 300
Cfr13I GGNCC 8 cut(s) 4, 249, 384, 469, 734, 848, 854, 1003
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 2 cut(s) 549, 936
CviAII CATG 2 cut(s) 133, 1045
CviQI GTAC 2 cut(s) 549, 936
DdeI CTNAG 1 cut(s) 279
DinI GGCGCC 1 cut(s) 40
DpnI GATC 3 cut(s) 111, 123, 594
DpnII GATC 3 cut(s) 109, 121, 592
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 797
Eco130I CCWWGG 3 cut(s) 264, 434, 985
Eco147I AGGCCT 1 cut(s) 712
Eco24I GRGCYC 2 cut(s) 799, 877
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 4 cut(s) 4, 384, 469, 734
Eco53kI GAGCTC 1 cut(s) 797
Eco88I CYCGRG 2 cut(s) 98, 729
EcoICRI GAGCTC 1 cut(s) 797
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoO109I RGGNCCY 1 cut(s) 384
EcoRII CCWGG 8 cut(s) 694, 766, 811, 850, 865, 931, 939, 1005
EcoT14I CCWWGG 3 cut(s) 264, 434, 985
EcoT38I GRGCYC 2 cut(s) 799, 877
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 3 cut(s) 264, 434, 985
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 2 cut(s) 136, 1048
FaqI GGGAC 1 cut(s) 370
FatI CATG 2 cut(s) 132, 1044
FauI CCCGC 1 cut(s) 240
FblI GTMKAC 1 cut(s) 949
Fnu4HI GCNGC 4 cut(s) 42, 73, 510, 620
FriOI GRGCYC 2 cut(s) 799, 877
Fsp4HI GCNGC 4 cut(s) 42, 73, 510, 620
FspBI CTAG 3 cut(s) 546, 792, 1041
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 4 cut(s) 40, 137, 224, 299
GluI GCNGC 4 cut(s) 42, 73, 510, 620
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 2 cut(s) 42, 226
HaeIII GGCC 8 cut(s) 206, 251, 263, 337, 712, 849, 856, 1005
HapII CCGG 3 cut(s) 27, 338, 410
HhaI GCGC 4 cut(s) 41, 138, 225, 300
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 2 cut(s) 136, 1048
Hin6I GCGC 4 cut(s) 39, 136, 223, 298
HinP1I GCGC 4 cut(s) 39, 136, 223, 298
HindIII AAGCTT 1 cut(s) 978
HinfI GANTC 2 cut(s) 81, 568
HpaII CCGG 3 cut(s) 27, 338, 410
HphI GGTGA 2 cut(s) 134, 931
Hpy166II GTNNAC 5 cut(s) 164, 612, 737, 773, 950
Hpy188I TCNGA 3 cut(s) 86, 149, 573
Hpy188III TCNNGA 3 cut(s) 100, 397, 410
Hpy8I GTNNAC 5 cut(s) 164, 612, 737, 773, 950
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 2 cut(s) 398, 714
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 614
HpyCH4V TGCA 6 cut(s) 181, 428, 509, 619, 773, 1000
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 248, 253, 295, 881
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 1 cut(s) 614
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 2 cut(s) 136, 1048
HspAI GCGC 4 cut(s) 39, 136, 223, 298
KasI GGCGCC 1 cut(s) 38
Kpn2I TCCGGA 1 cut(s) 409
KpnI GGTACC 1 cut(s) 939
Kzo9I GATC 3 cut(s) 109, 121, 592
LmnI GCTCC 4 cut(s) 697, 763, 802, 880
Lsp1109I GCAGC 2 cut(s) 521, 631
LweI GCATC 2 cut(s) 448, 987
MaeI CTAG 3 cut(s) 546, 792, 1041
MaeII ACGT 1 cut(s) 614
MaeIII GTNAC 2 cut(s) 194, 349
MalI GATC 3 cut(s) 111, 123, 594
MboI GATC 3 cut(s) 109, 121, 592
MboII GAAGA 5 cut(s) 385, 431, 491, 571, 1004
MflI RGATCY 2 cut(s) 121, 592
MhlI GDGCHC 4 cut(s) 694, 775, 799, 877
MluCI AATT 3 cut(s) 327, 528, 922
MluI ACGCGT 1 cut(s) 254
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 2 cut(s) 75, 562
MmeI TCCRAC 2 cut(s) 109, 496
MroI TCCGGA 1 cut(s) 409
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 3 cut(s) 537, 636, 1054
MslI CAYNNNNRTG 2 cut(s) 186, 917
MspI CCGG 3 cut(s) 27, 338, 410
MvaI CCWGG 8 cut(s) 696, 768, 813, 852, 867, 933, 941, 1007
MvnI CGCG 2 cut(s) 75, 256
MwoI GCNNNNNNNGC 5 cut(s) 47, 248, 253, 295, 881
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 2 cut(s) 27, 339
NdeII GATC 3 cut(s) 109, 121, 592
NlaIII CATG 2 cut(s) 136, 1048
NlaIV GGNNCC 9 cut(s) 40, 386, 470, 543, 594, 765, 817, 876, 937
NsbI TGCGCA 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 917
PaeR7I CTCGAG 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 889
PasI CCCWGGG 1 cut(s) 851
PceI AGGCCT 1 cut(s) 712
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 72, 105
PdmI GAANNNNTTC 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 694
PkrI GCNGC 4 cut(s) 43, 74, 511, 621
PleI GAGTC 2 cut(s) 75, 562
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 2 cut(s) 75, 562
PpuMI RGGWCCY 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 799
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 8 cut(s) 694, 766, 811, 850, 865, 931, 939, 1005
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 8 cut(s) 694, 766, 811, 850, 865, 931, 939, 1005
PspN4I GGNNCC 9 cut(s) 40, 386, 470, 543, 594, 765, 817, 876, 937
PspPI GGNCC 8 cut(s) 4, 249, 384, 469, 734, 848, 854, 1003
PspPPI RGGWCCY 1 cut(s) 384
PsuI RGATCY 2 cut(s) 121, 592
RsaI GTAC 2 cut(s) 550, 937
RsaNI GTAC 2 cut(s) 549, 936
RseI CAYNNNNRTG 2 cut(s) 186, 917
SacI GAGCTC 1 cut(s) 799
SaqAI TTAA 3 cut(s) 537, 636, 1054
SatI GCNGC 4 cut(s) 42, 73, 510, 620
Sau3AI GATC 3 cut(s) 109, 121, 592
Sau96I GGNCC 8 cut(s) 4, 249, 384, 469, 734, 848, 854, 1003
SchI GAGTC 2 cut(s) 75, 562
SduI GDGCHC 4 cut(s) 694, 775, 799, 877
SfaNI GCATC 2 cut(s) 448, 987
SfcI CTRYAG 1 cut(s) 343
SfoI GGCGCC 1 cut(s) 40
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 4 cut(s) 4, 384, 469, 734
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 186, 917
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 3 cut(s) 327, 528, 922
SseBI AGGCCT 1 cut(s) 712
SsiI CCGC 5 cut(s) 42, 48, 70, 73, 247
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 3 cut(s) 546, 792, 1041
SstI GAGCTC 1 cut(s) 799
StuI AGGCCT 1 cut(s) 712
StyI CCWWGG 3 cut(s) 264, 434, 985
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 617
TaqI TCGA 4 cut(s) 66, 99, 108, 566
TaqII GACCGA 1 cut(s) 21
TasI AATT 3 cut(s) 327, 528, 922
TatI WGTACW 1 cut(s) 548
TauI GCSGC 2 cut(s) 44, 75
Tru1I TTAA 3 cut(s) 537, 636, 1054
Tru9I TTAA 3 cut(s) 537, 636, 1054
TscAI CASTG 1 cut(s) 327
TseI GCWGC 2 cut(s) 509, 619
TspDTI ATGAA 1 cut(s) 121
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 327
VneI GTGCAC 1 cut(s) 771
VpaK11BI GGWCC 4 cut(s) 4, 384, 469, 734
XagI CCTNNNNNAGG 1 cut(s) 753
XapI RAATTY 1 cut(s) 922
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 3 cut(s) 546, 792, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.