Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013544

Physical Location & Seq

Genomic Coordinates Forward (+)

8353606 .. 8358349

Gene Structure 4744 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1055900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGAATTTAGGGTTTCACATGGAATCCCTATGTTCTCTGGCTCCTCACACCTCGCTTACCTCCATAACCAGCGTCACTGCTTCTCATCCCTCAAGAAACTCCATAAAACCGCTCAAGCCCGCGCTTTTGTCAATGGCGGAGGCGGTGGTTCTAAAGGTGATTCTTTTTTGTTGCCTGGTGCAACGGTGGCTACATTACTTATGCTCGGTGTTCTACATGGTCGTCGGATGTATGAAGACCGGAAGGCCGAGGAGGCACGAGAGAATGGAGCTGAACTTGAGTTTCAACCAGATGTAAAATCTAAATTTTTAAGGTTGCTGCCTCTGCGCTCCATTTCCAGATGTTGGGGATTATTGACAAGTGTGGAAATTCCAGTCTCTCTCCGCTCGCATGTTTATGGAGCGTGGGCTCGGGCATTCCATTCAAACTTGGAAGAAGCAGCTCTGCCACTGGATGAATACGCATCTTTACGAGAGTTCTTTGTTCGAACCTTGAAAGAGGGTTCCAGGCCTATTGACCCCGACCCACGATGTCTGGTTAGTCCTGTGGATGGTACAGTGTTAAGATTTGGGGAGCTTAGAGAAGCAGGAGCAATGATTGAACAAGTAAAAGGTTTTTCTTATTCTGTTTTTTCTCTGCTCGGTGCAAACTCATTTCTTCCTATGATAGCTAAAGGAGATATGCATGAGGAGAGTAGTGAACCAGAAAATACTTCCAGGGAGAAGAATAAAAAGTCATGGTTGAGAGTTTCATTGGCTTCCCCTAAAGTTTGGGACCCTGTGTCAGAACGTCCTATGAATGGCCTATTTTACTGTGTAATTTACTTGAAGCCTGGAGACTATCATCGTATTCATGCACCCGCTGATTGGAATGTTCTTGTCCGCCGTCACTTTTCGGGTCGTCTATTTCCTGTAAATGAACGTGCTACAAGAACAATCAGAAACCTTTATGTTGAAAATGAACGGGTTGTTCTTGAAGGTCTATGGAAAGAAGGATTTATGGCACTTGCTGCTATTGGAGCAACAAATATTGGTTCGATTGAGCTTTCCATTGAACCAGAACTTCAGACAAACCAGGCCAGAAAGAAGTTACTGCCCTCAGGGCCTCCAGAAGAACAGTTATACGAGCCTGATGGTATTGGTAGGATGCTCAAGAAAGGGGATGAGGTAGCCGCATTCAACATGGGATCAACTGTGGTGCTTGTCTTCCAGGCCCCCATATCACTATCACAAGAAAAACGGGATTCCTCATCAGAGTTCAAGTTTTTGGTGCAACGTGGGGATAGAGTCCGCGTCGGAGAAGCATTAGGGAGGTGGTATGATCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004609 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006508 GO:0006629 GO:0006644 GO:0006650 GO:0006656 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0010635 GO:0010636 GO:0010638 GO:0010821 GO:0010822 GO:0010954 GO:0016020 GO:0016021 GO:0016485 GO:0016540 GO:0016829 GO:0016830 GO:0016831 GO:0019222 GO:0019538 GO:0019637 GO:0019866 GO:0022603 GO:0030162 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031323 GO:0031325 GO:0031966 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0032592 GO:0033043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045017 GO:0045862 GO:0046470 GO:0046474 GO:0046486 GO:0048518 GO:0048522 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051604 GO:0060255 GO:0065007 GO:0070613 GO:0071704 GO:0080090 GO:0090407 GO:0097164 GO:0098573 GO:1901564 GO:1901566 GO:1901576 GO:1903317 GO:1903319

KEGG Pathways

Metabolic & Signaling

ko00564 ko01100 ko01110 map00564 map01100 map01110

Pfam Domains

Protein Families

PS_Dcarbxylase

Protein Analysis

443

Amino Acids

49.62

Weight (kDa)

8.87

Isoelectric Point (pI)

46.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PS_Dcarbxylase	PF02666	158 - 437	8.7e-52	Phosphatidylserine decarboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013544)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16700 AT4G16700
fragaria_vesca	FvH4_5g19150
malus_domestica	MD06G1055900.v1.1
prunus_persica	Prupe.5G066800_v2.0.a1 Prupe.5G066800_v2.0.a1 Prupe.5G066800_v2.0.a1
pyrus_communis	pycom06g04690
rosa_chinensis	RchiOBHm_Chr7g0203641
rosa_laevigata	RLG00000003513
rosa_multiflora	Rmu_sc0019220.1_g000001
rosa_roxburghii	Rroxscaffold_3G00253620
rosa_rugosa	Rorug07G0077900
rosa_samantha	Rh5CG208900 Rh7BG206800 Rh7CG219800 Rh7DG214400
rosa_wichuraiana	Rw7G018090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	345
AccBSI	CCGCTC	2 cut(s)	113, 387
AccII	CGCG	2 cut(s)	123, 1293
AciI	CCGC	9 cut(s)	111, 121, 138, 144, 385, 861, 883, 1173, 1291
AclWI	GGATC	1 cut(s)	1195
AcsI	RAATTY	3 cut(s)	5, 305, 369
AcuI	CTGAAG	1 cut(s)	1049
AfaI	GTAC	1 cut(s)	556
AfiI	CCNNNNNNNGG	4 cut(s)	345, 551, 800, 867
AhdI	GACNNNNNGTC	1 cut(s)	781
AjnI	CCWGG	6 cut(s)	175, 506, 716, 832, 1074, 1209
AluBI	AGCT	5 cut(s)	272, 443, 577, 671, 1045
AluI	AGCT	5 cut(s)	272, 443, 577, 671, 1045
Alw26I	GTCTC	2 cut(s)	382, 831
AlwI	GGATC	1 cut(s)	1195
Ama87I	CYCGRG	1 cut(s)	411
AoxI	GGCC	6 cut(s)	246, 509, 802, 1077, 1103, 1212
ApeKI	GCWGC	3 cut(s)	319, 440, 1010
ApoI	RAATTY	3 cut(s)	5, 305, 369
AspLEI	GCGC	2 cut(s)	125, 330
AspS9I	GGNCC	3 cut(s)	775, 1103, 1213
AsuHPI	GGTGA	1 cut(s)	170
AsuII	TTCGAA	1 cut(s)	487
AvaI	CYCGRG	1 cut(s)	411
AvaII	GGWCC	1 cut(s)	775
AxyI	CCTNAGG	1 cut(s)	1099
BanII	GRGCYC	1 cut(s)	412
BauI	CACGAG	1 cut(s)	258
BbsI	GAAGAC	2 cut(s)	243, 1198
BbvI	GCAGC	3 cut(s)	306, 452, 997
BccI	CCATC	2 cut(s)	545, 1127
BceAI	ACGGC	1 cut(s)	870
BciT130I	CCWGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
BclI	TGATCA	1 cut(s)	1321
BcoDI	GTCTC	2 cut(s)	382, 831
BglI	GCCNNNNNGGC	2 cut(s)	254, 1102
BisI	GCNGC	4 cut(s)	320, 441, 1011, 1173
BlsI	GCNGC	4 cut(s)	321, 442, 1012, 1174
Bme1390I	CCNGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
Bme18I	GGWCC	1 cut(s)	775
BmeRI	GACNNNNNGTC	1 cut(s)	781
BmeT110I	CYCGRG	1 cut(s)	411
BmgT120I	GGNCC	3 cut(s)	775, 1103, 1213
BmiI	GGNNCC	5 cut(s)	43, 505, 776, 777, 1215
BmrFI	CCNGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
BmsI	GCATC	2 cut(s)	473, 1137
BpiI	GAAGAC	2 cut(s)	243, 1198
BpmI	CTGGAG	2 cut(s)	855, 1092
Bpu14I	TTCGAA	1 cut(s)	487
BpuEI	CTTGAG	4 cut(s)	77, 99, 299, 1136
BsaJI	CCNNGG	2 cut(s)	249, 717
BsaWI	WCCGGW	1 cut(s)	240
Bsc4I	CCNNNNNNNGG	4 cut(s)	345, 551, 800, 867
Bse1I	ACTGG	2 cut(s)	374, 456
Bse21I	CCTNAGG	1 cut(s)	1099
Bse3DI	GCAATG	1 cut(s)	600
BseBI	CCWGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
BseDI	CCNNGG	2 cut(s)	249, 717
BseGI	GGATG	6 cut(s)	86, 234, 460, 556, 1152, 1168
BseLI	CCNNNNNNNGG	4 cut(s)	345, 551, 800, 867
BseMI	GCAATG	1 cut(s)	600
BseMII	CTCAG	1 cut(s)	1113
BseNI	ACTGG	2 cut(s)	374, 456
BseRI	GAGGAG	3 cut(s)	34, 266, 704
BseXI	GCAGC	3 cut(s)	306, 452, 997
Bsh1236I	CGCG	2 cut(s)	123, 1293
BshFI	GGCC	6 cut(s)	248, 511, 804, 1079, 1105, 1214
BsiHKCI	CYCGRG	1 cut(s)	411
BsiSI	CCGG	1 cut(s)	241
BslFI	GGGAC	1 cut(s)	788
BslI	CCNNNNNNNGG	4 cut(s)	345, 551, 800, 867
BsmAI	GTCTC	2 cut(s)	382, 831
BsmFI	GGGAC	1 cut(s)	788
BsmI	GAATGC	2 cut(s)	416, 1175
BsnI	GGCC	6 cut(s)	248, 511, 804, 1079, 1105, 1214
BsoBI	CYCGRG	1 cut(s)	411
Bsp119I	TTCGAA	1 cut(s)	487
Bsp1286I	GDGCHC	1 cut(s)	412
Bsp143I	GATC	2 cut(s)	1187, 1321
BspACI	CCGC	9 cut(s)	111, 121, 138, 144, 385, 861, 883, 1173, 1291
BspANI	GGCC	6 cut(s)	248, 511, 804, 1079, 1105, 1214
BspCNI	CTCAG	1 cut(s)	1112
BspFNI	CGCG	2 cut(s)	123, 1293
BspLI	GGNNCC	5 cut(s)	43, 505, 776, 777, 1215
BspPI	GGATC	1 cut(s)	1195
BspT104I	TTCGAA	1 cut(s)	487
BsrBI	CCGCTC	2 cut(s)	113, 387
BsrDI	GCAATG	1 cut(s)	600
BsrI	ACTGG	2 cut(s)	374, 456
BssECI	CCNNGG	2 cut(s)	249, 717
BssMI	GATC	2 cut(s)	1187, 1321
BssSI	CACGAG	1 cut(s)	258
Bst2BI	CACGAG	1 cut(s)	258
Bst2UI	CCWGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
Bst4CI	ACNGT	5 cut(s)	187, 559, 815, 1119, 1195
BstAPI	GCANNNNNTGC	1 cut(s)	1010
BstBI	TTCGAA	1 cut(s)	487
BstC8I	GCNNGC	2 cut(s)	121, 389
BstDEI	CTNAG	2 cut(s)	578, 1099
BstF5I	GGATG	6 cut(s)	86, 234, 460, 556, 1152, 1168
BstFNI	CGCG	2 cut(s)	123, 1293
BstHHI	GCGC	2 cut(s)	125, 330
BstKTI	GATC	2 cut(s)	1190, 1324
BstMAI	GTCTC	2 cut(s)	382, 831
BstMBI	GATC	2 cut(s)	1187, 1321
BstMWI	GCNNNNNNNGC	6 cut(s)	188, 254, 325, 1010, 1019, 1102
BstNI	CCWGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
BstNSI	RCATGY	1 cut(s)	395
BstSCI	CCNGG	6 cut(s)	175, 506, 716, 832, 1074, 1209
BstUI	CGCG	2 cut(s)	123, 1293
BstV1I	GCAGC	3 cut(s)	306, 452, 997
BstV2I	GAAGAC	2 cut(s)	243, 1198
Bsu36I	CCTNAGG	1 cut(s)	1099
BsuRI	GGCC	6 cut(s)	248, 511, 804, 1079, 1105, 1214
BtsCI	GGATG	6 cut(s)	86, 234, 460, 556, 1152, 1168
BtsI	GCAGTG	1 cut(s)	76
BtsIMutI	CAGTG	3 cut(s)	76, 449, 564
Cac8I	GCNNGC	2 cut(s)	121, 389
CfoI	GCGC	2 cut(s)	125, 330
Cfr13I	GGNCC	3 cut(s)	775, 1103, 1213
CseI	GACGC	2 cut(s)	62, 1282
Csp6I	GTAC	1 cut(s)	555
CviAII	CATG	7 cut(s)	20, 218, 392, 686, 738, 854, 1183
CviQI	GTAC	1 cut(s)	555
DdeI	CTNAG	2 cut(s)	578, 1099
DpnI	GATC	2 cut(s)	1189, 1323
DpnII	GATC	2 cut(s)	1187, 1321
DriI	GACNNNNNGTC	1 cut(s)	781
Eam1105I	GACNNNNNGTC	1 cut(s)	781
EciI	GGCGGA	2 cut(s)	153, 872
Eco147I	AGGCCT	1 cut(s)	511
Eco24I	GRGCYC	1 cut(s)	412
Eco47I	GGWCC	1 cut(s)	775
Eco57I	CTGAAG	1 cut(s)	1049
Eco81I	CCTNAGG	1 cut(s)	1099
Eco88I	CYCGRG	1 cut(s)	411
EcoO109I	RGGNCCY	3 cut(s)	775, 1103, 1213
EcoRII	CCWGG	6 cut(s)	175, 506, 716, 832, 1074, 1209
EcoT22I	ATGCAT	1 cut(s)	687
EcoT38I	GRGCYC	1 cut(s)	412
FaeI	CATG	7 cut(s)	23, 221, 395, 689, 741, 857, 1186
FaqI	GGGAC	1 cut(s)	788
FatI	CATG	7 cut(s)	19, 217, 391, 685, 737, 853, 1182
FauI	CCCGC	2 cut(s)	128, 868
FbaI	TGATCA	1 cut(s)	1321
Fnu4HI	GCNGC	4 cut(s)	320, 441, 1011, 1173
FokI	GGATG	6 cut(s)	73, 241, 467, 563, 1159, 1175
FriOI	GRGCYC	1 cut(s)	412
Fsp4HI	GCNGC	4 cut(s)	320, 441, 1011, 1173
GlaI	GCGC	2 cut(s)	124, 329
GluI	GCNGC	4 cut(s)	320, 441, 1011, 1173
GsuI	CTGGAG	2 cut(s)	855, 1092
HaeIII	GGCC	6 cut(s)	248, 511, 804, 1079, 1105, 1214
HapII	CCGG	1 cut(s)	241
HgaI	GACGC	2 cut(s)	62, 1282
HhaI	GCGC	2 cut(s)	125, 330
Hin1II	CATG	7 cut(s)	23, 221, 395, 689, 741, 857, 1186
Hin6I	GCGC	2 cut(s)	123, 328
HinP1I	GCGC	2 cut(s)	123, 328
HinfI	GANTC	4 cut(s)	24, 161, 1244, 1287
HpaII	CCGG	1 cut(s)	241
HphI	GGTGA	1 cut(s)	170
Hpy166II	GTNNAC	1 cut(s)	701
Hpy188I	TCNGA	6 cut(s)	228, 787, 941, 1068, 1255, 1298
Hpy188III	TCNNGA	5 cut(s)	94, 339, 974, 1109, 1153
Hpy8I	GTNNAC	1 cut(s)	701
Hpy99I	CGWCG	2 cut(s)	228, 1298
HpyAV	CCTTC	3 cut(s)	238, 971, 986
HpyCH4III	ACNGT	5 cut(s)	187, 559, 815, 1119, 1195
HpyCH4IV	ACGT	3 cut(s)	790, 922, 1276
HpyCH4V	TGCA	5 cut(s)	182, 647, 685, 857, 1273
HpyF10VI	GCNNNNNNNGC	6 cut(s)	188, 254, 325, 1010, 1019, 1102
HpyF3I	CTNAG	2 cut(s)	578, 1099
HpySE526I	ACGT	3 cut(s)	790, 922, 1276
Hsp92II	CATG	7 cut(s)	23, 221, 395, 689, 741, 857, 1186
HspAI	GCGC	2 cut(s)	123, 328
KflI	GGGWCCC	1 cut(s)	775
Ksp22I	TGATCA	1 cut(s)	1321
Kzo9I	GATC	2 cut(s)	1187, 1321
LmnI	GCTCC	7 cut(s)	47, 269, 335, 401, 574, 590, 1019
Lsp1109I	GCAGC	3 cut(s)	306, 452, 997
LweI	GCATC	2 cut(s)	473, 1137
MaeII	ACGT	3 cut(s)	790, 922, 1276
MaeIII	GTNAC	3 cut(s)	74, 887, 1089
MalI	GATC	2 cut(s)	1189, 1323
MbiI	CCGCTC	2 cut(s)	113, 387
MboI	GATC	2 cut(s)	1187, 1321
MboII	GAAGA	6 cut(s)	248, 446, 650, 736, 1124, 1198
MhlI	GDGCHC	1 cut(s)	412
MluCI	AATT	4 cut(s)	5, 305, 369, 819
MlyI	GAGTC	1 cut(s)	1296
MmeI	TCCRAC	2 cut(s)	206, 1276
Mph1103I	ATGCAT	1 cut(s)	687
MseI	TTAA	2 cut(s)	311, 563
MslI	CAYNNNNRTG	1 cut(s)	396
MspA1I	CMGCKG	1 cut(s)	863
MspI	CCGG	1 cut(s)	241
MspR9I	CCNGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
Mva1269I	GAATGC	2 cut(s)	416, 1175
MvaI	CCWGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
MvnI	CGCG	2 cut(s)	123, 1293
MwoI	GCNNNNNNNGC	6 cut(s)	188, 254, 325, 1010, 1019, 1102
NdeII	GATC	2 cut(s)	1187, 1321
NlaIII	CATG	7 cut(s)	23, 221, 395, 689, 741, 857, 1186
NlaIV	GGNNCC	5 cut(s)	43, 505, 776, 777, 1215
NmeAIII	GCCGAG	1 cut(s)	274
NmuCI	GTSAC	2 cut(s)	74, 887
NsiI	ATGCAT	1 cut(s)	687
NspI	RCATGY	1 cut(s)	395
NspV	TTCGAA	1 cut(s)	487
PceI	AGGCCT	1 cut(s)	511
PctI	GAATGC	2 cut(s)	416, 1175
PfeI	GAWTC	3 cut(s)	24, 161, 1244
PflMI	CCANNNNNTGG	1 cut(s)	345
PkrI	GCNGC	4 cut(s)	321, 442, 1012, 1174
PleI	GAGTC	1 cut(s)	1295
PpsI	GAGTC	1 cut(s)	1295
PpuMI	RGGWCCY	1 cut(s)	775
Psp5II	RGGWCCY	1 cut(s)	775
Psp6I	CCWGG	6 cut(s)	175, 506, 716, 832, 1074, 1209
PspGI	CCWGG	6 cut(s)	175, 506, 716, 832, 1074, 1209
PspN4I	GGNNCC	5 cut(s)	43, 505, 776, 777, 1215
PspPI	GGNCC	3 cut(s)	775, 1103, 1213
PspPPI	RGGWCCY	1 cut(s)	775
RsaI	GTAC	1 cut(s)	556
RsaNI	GTAC	1 cut(s)	555
RseI	CAYNNNNRTG	1 cut(s)	396
SaqAI	TTAA	2 cut(s)	311, 563
SatI	GCNGC	4 cut(s)	320, 441, 1011, 1173
Sau3AI	GATC	2 cut(s)	1187, 1321
Sau96I	GGNCC	3 cut(s)	775, 1103, 1213
SchI	GAGTC	1 cut(s)	1296
ScrFI	CCNGG	6 cut(s)	177, 508, 718, 834, 1076, 1211
SduI	GDGCHC	1 cut(s)	412
SfaNI	GCATC	2 cut(s)	473, 1137
SfuI	TTCGAA	1 cut(s)	487
SinI	GGWCC	1 cut(s)	775
SmiMI	CAYNNNNRTG	1 cut(s)	396
SmlI	CTYRAG	4 cut(s)	92, 114, 278, 1151
SmoI	CTYRAG	4 cut(s)	92, 114, 278, 1151
Sse9I	AATT	4 cut(s)	5, 305, 369, 819
SseBI	AGGCCT	1 cut(s)	511
SsiI	CCGC	9 cut(s)	111, 121, 138, 144, 385, 861, 883, 1173, 1291
SspI	AATATT	1 cut(s)	1030
StuI	AGGCCT	1 cut(s)	511
StyD4I	CCNGG	6 cut(s)	175, 506, 716, 832, 1074, 1209
TaaI	ACNGT	5 cut(s)	187, 559, 815, 1119, 1195
TaiI	ACGT	3 cut(s)	793, 925, 1279
TaqI	TCGA	2 cut(s)	487, 1037
TasI	AATT	4 cut(s)	5, 305, 369, 819
TauI	GCSGC	1 cut(s)	1175
TfiI	GAWTC	3 cut(s)	24, 161, 1244
Tru1I	TTAA	2 cut(s)	311, 563
Tru9I	TTAA	2 cut(s)	311, 563
TscAI	CASTG	3 cut(s)	83, 456, 564
TseFI	GTSAC	2 cut(s)	74, 887
TseI	GCWGC	3 cut(s)	319, 440, 1010
Tsp45I	GTSAC	2 cut(s)	74, 887
TspDTI	ATGAA	7 cut(s)	249, 471, 741, 812, 842, 933, 975
TspRI	CASTG	3 cut(s)	83, 456, 564
Van91I	CCANNNNNTGG	1 cut(s)	345
VpaK11BI	GGWCC	1 cut(s)	775
XapI	RAATTY	3 cut(s)	5, 305, 369
XceI	RCATGY	1 cut(s)	395
Zsp2I	ATGCAT	1 cut(s)	687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.