Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0014367

Physical Location & Seq

Genomic Coordinates Forward (+)

9124468 .. 9133106

Gene Structure 8639 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1059300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGCTGCTGCCTTAGCACCATCGCCTTCTCTCCGGTTCAATCCTTACTCCACCCTCCGGCTCCTCTCCTTCTACCCCAGAACCCCACTTCCCCGCCGCACCCCAACTCCAATTTTCCCGCCATTTCTTCCCCGCCTCTCCCGCGCCGCCGCCGTCCCTCGCCGGAGGTCCTTCTGTACCCTCATCTCCGCAGCTCTCCACTCCGGCGGAGCTGCCGAGAGGACGAAACCGGAAATTGCTGAAGATCGGAGGTCCGAAGGCGGCAACAACAGAGTTGGGGAATTCAGGAAGAAGCTCAAGGTTGTGGATGTCAAGGGAGGGTCCGATGAGGGGCTCGAGCGCCTGGGCCAGAGCTTGGTGGTTATGGGTTGGGTTCGTACTCTCCGAGTTCAAAGTAGCGTTACATTCATTGAGGTTAATGATGGGTCGTGTCTCTCAAACATGCAATGTGTATTGGGCTCAGATGCTGAAGGTTATGATCAGGTAGAATCTGGCTCAATCACAACTGGTGCATCAATATGGGTGCAAGGGATAATATCTGCCAGCCAAGGATCAAAACAGAAAGTGGAATTGAAGGTCAACAAAATTGTTCTGGTTGGAAAGAGTGATCCTTCGTATCCCATCCAAAAGAAAAGGGTCAGTAGAGAATTTTTGAGAACAAAAGCTCATCTTCGGCCAAGAACAAATACTTTTGGTGCGGTTGCAAGAGTGAGAAATGCTTTGGCATATGCTACTCACAAGTTTTTCCAGGAAAATGGATTTGTATGGGTTTCCAGTCCCATCATTACTGCTTCAGATTGCGAAGGAGCAGGTGAACAATTTTGTGTGACTACTTTGATTCCAAGTTCTAGAGAAGCCTCTCATTCTCCTGTGGATGGCATTCCAAAATCAAATGATGGCCTAGTTGATTGGTCACAAGATTTTTTTGGGAAGCCAGCATTTTTGACTGTCTCTGGCCAACTCAATGCTGAAACCTATGCTACTGCTCTGTCTGATGTATATACATTTGGCCCCACATTTCGGGCTGAAAATTCCAACACTTCTAGGCACCTGGCTGAATTTTGGATGATTGAACCGGAACTTGCATTTGCTGATCTGAATGATGACATGGCCTGTGCAAGTGCCTATCTCCAGTATGTAGTGAGACACATCCTCGAAAATTGCAAAGAAGACATGGAATTTTTCAACACTTGGATTGAGAAAGGAATCATTGGTCGATTGAGTGATGTGGCTGAGAAGGACTTCGTGCAGTTGACTTATACTGATGCAGTTGAACTTCTTCTTAAAGCAAATAAGAAATTTGAATTCCCAGTCAAGTGGGGTTGTGATTTGCAGAGTGAGCATGAACGTTATTTAACTGAAGAGGCATTTAGAGGATGCCCTGTAATTGTCAGAGATTATCCAAAGGAGATCAAAGCATTTTATATGCGGCAAAATGATGATGGAAAGACTGTCGCAGCAATGGATATGTTGGTTCCCCGGGTTGGCGAGCTTATTGGTGGAAGCCAGAGAGAAGAGAGGCTTGAATATTTAGAAGATCGGTTGGATGAATTAAAGCTGGAGAAGGAAAGCTACTGGTGGTATCTTGATTTGCGTCGTTACGGTTCAGTTCCTCATGCAGGCTTCGGTTTGGGTTTTGAAAGGCTTGTACAGTTTGCAACTGGGATAGACAACATTCGAGATACTATTCCGTTTCCCCGGGCACCTGGTTCGGCAGAGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004816 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006421 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

575

Amino Acids

64.29

Weight (kDa)

6.51

Isoelectric Point (pI)

45.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	216 - 567	2.4e-60	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014367)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17300 AT4G17300
fragaria_vesca	FvH4_1g25310
malus_domestica	MD06G1059300.v1.1
prunus_persica	Prupe.5G070100_v2.0.a1 Prupe.5G070100_v2.0.a1
pyrus_communis	pycom06g04930
rosa_chinensis	RchiOBHm_Chr2g0123321
rosa_multiflora	Rmu_sc0001917.1_g000001 Rmu_sc0021262.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120780
rosa_rugosa	Rorug02G0244500
rosa_samantha	Rh2AG303600 Rh2BG311700 Rh2CG290200 Rh2DG327600
rosa_wichuraiana	Rw2G024300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	800
Acc36I	ACCTGC	1 cut(s)	800
AccB1I	GGYRCC	2 cut(s)	1047, 1702
AccB7I	CCANNNNNTGG	1 cut(s)	355
AccII	CGCG	1 cut(s)	144
AclI	AACGTT	1 cut(s)	1348
AclWI	GGATC	2 cut(s)	559, 602
AcoI	YGGCCR	2 cut(s)	674, 955
AcsI	RAATTY	7 cut(s)	281, 647, 1030, 1058, 1178, 1298, 1304
AcuI	CTGAAG	4 cut(s)	261, 489, 777, 1380
AfaI	GTAC	3 cut(s)	178, 379, 1650
AfiI	CCNNNNNNNGG	7 cut(s)	57, 330, 355, 875, 1020, 1484, 1619
AgsI	TTSAA	9 cut(s)	40, 392, 574, 1073, 1186, 1274, 1304, 1526, 1640
AjnI	CCWGG	4 cut(s)	342, 747, 1050, 1705
AloI	GAACNNNNNNTCC	2 cut(s)	73, 105
AluBI	AGCT	8 cut(s)	194, 212, 295, 354, 665, 1492, 1558, 1572
AluI	AGCT	8 cut(s)	194, 212, 295, 354, 665, 1492, 1558, 1572
Alw26I	GTCTC	3 cut(s)	437, 955, 1138
AlwI	GGATC	2 cut(s)	559, 602
AlwNI	CAGNNNCTG	1 cut(s)	467
Ama87I	CYCGRG	3 cut(s)	335, 1479, 1698
AoxI	GGCC	6 cut(s)	346, 674, 898, 955, 1009, 1110
ApeKI	GCWGC	5 cut(s)	5, 8, 191, 212, 1457
ApoI	RAATTY	7 cut(s)	281, 647, 1030, 1058, 1178, 1298, 1304
Asp700I	GAANNNNTTC	2 cut(s)	1241, 1278
AspLEI	GCGC	2 cut(s)	146, 342
AspS9I	GGNCC	5 cut(s)	168, 252, 321, 346, 1010
AsuC2I	CCSGG	4 cut(s)	1480, 1481, 1699, 1700
AsuHPI	GGTGA	1 cut(s)	824
AvaI	CYCGRG	3 cut(s)	335, 1479, 1698
AvaII	GGWCC	3 cut(s)	168, 252, 321
BaeGI	GKGCMC	1 cut(s)	1705
BalI	TGGCCA	1 cut(s)	957
BanI	GGYRCC	2 cut(s)	1047, 1702
BanII	GRGCYC	2 cut(s)	336, 461
BarI	GAAGNNNNNNTAC	2 cut(s)	1556, 1588
BbsI	GAAGAC	1 cut(s)	1176
BbvI	GCAGC	3 cut(s)	199, 203, 1469
BccI	CCATC	7 cut(s)	28, 416, 629, 788, 869, 890, 1436
BceAI	ACGGC	1 cut(s)	137
BcgI	CGANNNNNNTGC	1 cut(s)	1692
BciT130I	CCWGG	4 cut(s)	344, 749, 1052, 1707
BciVI	GTATCC	1 cut(s)	627
BclI	TGATCA	1 cut(s)	478
BcnI	CCSGG	4 cut(s)	1480, 1481, 1699, 1700
BcoDI	GTCTC	3 cut(s)	437, 955, 1138
BfaI	CTAG	3 cut(s)	849, 902, 1044
BfoI	RGCGCY	1 cut(s)	343
BfuAI	ACCTGC	1 cut(s)	800
BfuI	GTATCC	1 cut(s)	627
Bme1390I	CCNGG	8 cut(s)	344, 749, 1052, 1480, 1481, 1699, 1700, 1707
Bme18I	GGWCC	3 cut(s)	168, 252, 321
BmeT110I	CYCGRG	3 cut(s)	335, 1479, 1698
BmgT120I	GGNCC	5 cut(s)	168, 252, 321, 346, 1010
BmiI	GGNNCC	6 cut(s)	62, 322, 1012, 1049, 1476, 1704
BmrFI	CCNGG	8 cut(s)	344, 749, 1052, 1480, 1481, 1699, 1700, 1707
BmrI	ACTGGG	2 cut(s)	1304, 1671
BmsI	GCATC	4 cut(s)	454, 521, 1255, 1367
BmuI	ACTGGG	2 cut(s)	1304, 1671
BpiI	GAAGAC	1 cut(s)	1176
BpmI	CTGGAG	2 cut(s)	1115, 1580
Bpu10I	CCTNAGC	1 cut(s)	13
BpuEI	CTTGAG	1 cut(s)	281
BpuMI	CCSGG	4 cut(s)	1480, 1481, 1699, 1700
BsaJI	CCNNGG	6 cut(s)	343, 547, 1478, 1479, 1697, 1698
BsaWI	WCCGGW	3 cut(s)	33, 229, 1075
BsaXI	ACNNNNNCTCC	2 cut(s)	91, 121
Bsc4I	CCNNNNNNNGG	7 cut(s)	57, 330, 355, 875, 1020, 1484, 1619
Bse1I	ACTGG	6 cut(s)	511, 774, 1132, 1310, 1580, 1666
Bse3DI	GCAATG	2 cut(s)	452, 1467
BseBI	CCWGG	4 cut(s)	344, 749, 1052, 1707
BseDI	CCNNGG	6 cut(s)	343, 547, 1478, 1479, 1697, 1698
BseGI	GGATG	7 cut(s)	313, 621, 880, 1071, 1149, 1382, 1552
BseLI	CCNNNNNNNGG	7 cut(s)	57, 330, 355, 875, 1020, 1484, 1619
BseMI	GCAATG	2 cut(s)	452, 1467
BseMII	CTCAG	2 cut(s)	474, 1224
BseNI	ACTGG	6 cut(s)	511, 774, 1132, 1310, 1580, 1666
BseRI	GAGGAG	1 cut(s)	53
BseSI	GKGCMC	1 cut(s)	1705
BseXI	GCAGC	3 cut(s)	199, 203, 1469
BsgI	GTGCAG	1 cut(s)	1268
Bsh1236I	CGCG	1 cut(s)	144
BshFI	GGCC	6 cut(s)	348, 676, 900, 957, 1011, 1112
BshNI	GGYRCC	2 cut(s)	1047, 1702
BsiHKCI	CYCGRG	3 cut(s)	335, 1479, 1698
BsiSI	CCGG	8 cut(s)	34, 58, 163, 204, 230, 1076, 1480, 1699
BslFI	GGGAC	2 cut(s)	140, 762
BslI	CCNNNNNNNGG	7 cut(s)	57, 330, 355, 875, 1020, 1484, 1619
BsmAI	GTCTC	3 cut(s)	437, 955, 1138
BsmFI	GGGAC	2 cut(s)	140, 762
BsmI	GAATGC	1 cut(s)	879
BsnI	GGCC	6 cut(s)	348, 676, 900, 957, 1011, 1112
BsoBI	CYCGRG	3 cut(s)	335, 1479, 1698
Bsp1286I	GDGCHC	3 cut(s)	336, 461, 1705
Bsp1407I	TGTACA	1 cut(s)	1648
Bsp143I	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
BspANI	GGCC	6 cut(s)	348, 676, 900, 957, 1011, 1112
BspCNI	CTCAG	2 cut(s)	473, 1225
BspFNI	CGCG	1 cut(s)	144
BspLI	GGNNCC	6 cut(s)	62, 322, 1012, 1049, 1476, 1704
BspMI	ACCTGC	1 cut(s)	800
BspPI	GGATC	2 cut(s)	559, 602
BspT107I	GGYRCC	2 cut(s)	1047, 1702
BsrDI	GCAATG	2 cut(s)	452, 1467
BsrGI	TGTACA	1 cut(s)	1648
BsrI	ACTGG	6 cut(s)	511, 774, 1132, 1310, 1580, 1666
BssECI	CCNNGG	6 cut(s)	343, 547, 1478, 1479, 1697, 1698
BssMI	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
BssT1I	CCWWGG	1 cut(s)	547
Bst2UI	CCWGG	4 cut(s)	344, 749, 1052, 1707
Bst4CI	ACNGT	4 cut(s)	949, 1453, 1604, 1653
Bst6I	CTCTTC	2 cut(s)	1356, 1509
BstAUI	TGTACA	1 cut(s)	1648
BstC8I	GCNNGC	4 cut(s)	544, 936, 1490, 1621
BstDEI	CTNAG	3 cut(s)	13, 460, 1233
BstENI	CCTNNNNNAGG	1 cut(s)	1617
BstF5I	GGATG	7 cut(s)	313, 621, 880, 1071, 1149, 1382, 1552
BstFNI	CGCG	1 cut(s)	144
BstH2I	RGCGCY	1 cut(s)	343
BstHHI	GCGC	2 cut(s)	146, 342
BstKTI	GATC	7 cut(s)	247, 481, 554, 610, 1096, 1413, 1540
BstMAI	GTCTC	3 cut(s)	437, 955, 1138
BstMBI	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 141, 1339
BstNI	CCWGG	4 cut(s)	344, 749, 1052, 1707
BstNSI	RCATGY	1 cut(s)	445
BstSCI	CCNGG	8 cut(s)	342, 747, 1050, 1478, 1479, 1697, 1698, 1705
BstSLI	GKGCMC	1 cut(s)	1705
BstUI	CGCG	1 cut(s)	144
BstV1I	GCAGC	3 cut(s)	199, 203, 1469
BstV2I	GAAGAC	1 cut(s)	1176
BstXI	CCANNNNNNTGG	2 cut(s)	755, 1317
BsuI	GTATCC	1 cut(s)	627
BsuRI	GGCC	6 cut(s)	348, 676, 900, 957, 1011, 1112
BtgZI	GCGATG	1 cut(s)	6
BtsCI	GGATG	7 cut(s)	313, 621, 880, 1071, 1149, 1382, 1552
BveI	ACCTGC	1 cut(s)	800
Cac8I	GCNNGC	4 cut(s)	544, 936, 1490, 1621
CaiI	CAGNNNCTG	1 cut(s)	467
CfoI	GCGC	2 cut(s)	146, 342
Cfr13I	GGNCC	5 cut(s)	168, 252, 321, 346, 1010
Cfr9I	CCCGGG	2 cut(s)	1479, 1698
CseI	GACGC	1 cut(s)	1583
CsiI	ACCWGGT	1 cut(s)	1705
Csp6I	GTAC	3 cut(s)	177, 378, 1649
CviAII	CATG	5 cut(s)	442, 1108, 1174, 1343, 1616
CviQI	GTAC	3 cut(s)	177, 378, 1649
DdeI	CTNAG	3 cut(s)	13, 460, 1233
DpnI	GATC	7 cut(s)	246, 480, 553, 609, 1095, 1412, 1539
DpnII	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
EaeI	YGGCCR	2 cut(s)	674, 955
Eam1104I	CTCTTC	2 cut(s)	1356, 1509
EarI	CTCTTC	2 cut(s)	1356, 1509
EciI	GGCGGA	1 cut(s)	222
Eco130I	CCWWGG	1 cut(s)	547
Eco24I	GRGCYC	2 cut(s)	336, 461
Eco47I	GGWCC	3 cut(s)	168, 252, 321
Eco57I	CTGAAG	4 cut(s)	261, 489, 777, 1380
Eco88I	CYCGRG	3 cut(s)	335, 1479, 1698
EcoNI	CCTNNNNNAGG	1 cut(s)	1617
EcoO109I	RGGNCCY	1 cut(s)	168
EcoRI	GAATTC	2 cut(s)	281, 1304
EcoRII	CCWGG	4 cut(s)	342, 747, 1050, 1705
EcoT14I	CCWWGG	1 cut(s)	547
EcoT38I	GRGCYC	2 cut(s)	336, 461
ErhI	CCWWGG	1 cut(s)	547
FaeI	CATG	5 cut(s)	445, 1111, 1177, 1346, 1619
FalI	AAGNNNNNCTT	4 cut(s)	654, 686, 1506, 1538
FaqI	GGGAC	2 cut(s)	140, 762
FatI	CATG	5 cut(s)	441, 1107, 1173, 1342, 1615
FauI	CCCGC	4 cut(s)	101, 126, 140, 149
FauNDI	CATATG	1 cut(s)	727
FbaI	TGATCA	1 cut(s)	478
FokI	GGATG	7 cut(s)	320, 608, 887, 1078, 1136, 1389, 1559
FriOI	GRGCYC	2 cut(s)	336, 461
FspBI	CTAG	3 cut(s)	849, 902, 1044
GlaI	GCGC	2 cut(s)	145, 341
GsuI	CTGGAG	2 cut(s)	1115, 1580
HaeII	RGCGCY	1 cut(s)	343
HaeIII	GGCC	6 cut(s)	348, 676, 900, 957, 1011, 1112
HapII	CCGG	8 cut(s)	34, 58, 163, 204, 230, 1076, 1480, 1699
HgaI	GACGC	1 cut(s)	1583
HhaI	GCGC	2 cut(s)	146, 342
Hin1II	CATG	5 cut(s)	445, 1111, 1177, 1346, 1619
Hin6I	GCGC	2 cut(s)	144, 340
HinP1I	GCGC	2 cut(s)	144, 340
HincII	GTYRAC	2 cut(s)	580, 1254
HindII	GTYRAC	2 cut(s)	580, 1254
HinfI	GANTC	3 cut(s)	488, 838, 1206
HpaII	CCGG	8 cut(s)	34, 58, 163, 204, 230, 1076, 1480, 1699
HphI	GGTGA	1 cut(s)	824
Hpy166II	GTNNAC	3 cut(s)	580, 815, 1254
Hpy188I	TCNGA	9 cut(s)	249, 256, 325, 386, 463, 796, 994, 1098, 1394
Hpy188III	TCNNGA	4 cut(s)	286, 849, 1586, 1679
Hpy8I	GTNNAC	3 cut(s)	580, 815, 1254
Hpy99I	CGWCG	1 cut(s)	1599
HpyCH4III	ACNGT	4 cut(s)	949, 1453, 1604, 1653
HpyCH4IV	ACGT	1 cut(s)	1348
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 141, 1339
HpyF3I	CTNAG	3 cut(s)	13, 460, 1233
HpySE526I	ACGT	1 cut(s)	1348
Hsp92II	CATG	5 cut(s)	445, 1111, 1177, 1346, 1619
HspAI	GCGC	2 cut(s)	144, 340
Ksp22I	TGATCA	1 cut(s)	478
Kzo9I	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
LmnI	GCTCC	3 cut(s)	66, 209, 806
Lsp1109I	GCAGC	3 cut(s)	199, 203, 1469
LweI	GCATC	4 cut(s)	454, 521, 1255, 1367
MabI	ACCWGGT	1 cut(s)	1705
MaeI	CTAG	3 cut(s)	849, 902, 1044
MaeII	ACGT	1 cut(s)	1348
MaeIII	GTNAC	4 cut(s)	400, 826, 912, 1598
MalI	GATC	7 cut(s)	246, 480, 553, 609, 1095, 1412, 1539
MboI	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
MboII	GAAGA	9 cut(s)	119, 254, 301, 662, 1181, 1271, 1373, 1526, 1547
MhlI	GDGCHC	3 cut(s)	336, 461, 1705
MlsI	TGGCCA	1 cut(s)	957
MluNI	TGGCCA	1 cut(s)	957
MmeI	TCCRAC	3 cut(s)	577, 1059, 1524
Mox20I	TGGCCA	1 cut(s)	957
MroXI	GAANNNNTTC	2 cut(s)	1241, 1278
MscI	TGGCCA	1 cut(s)	957
MseI	TTAA	4 cut(s)	417, 1284, 1355, 1553
MslI	CAYNNNNRTG	1 cut(s)	517
Msp20I	TGGCCA	1 cut(s)	957
MspI	CCGG	8 cut(s)	34, 58, 163, 204, 230, 1076, 1480, 1699
MspR9I	CCNGG	8 cut(s)	344, 749, 1052, 1480, 1481, 1699, 1700, 1707
Mva1269I	GAATGC	1 cut(s)	879
MvaI	CCWGG	4 cut(s)	344, 749, 1052, 1707
MvnI	CGCG	1 cut(s)	144
MwoI	GCNNNNNNNGC	3 cut(s)	14, 141, 1339
NciI	CCSGG	4 cut(s)	1480, 1481, 1699, 1700
NdeI	CATATG	1 cut(s)	727
NdeII	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
NlaIII	CATG	5 cut(s)	445, 1111, 1177, 1346, 1619
NlaIV	GGNNCC	6 cut(s)	62, 322, 1012, 1049, 1476, 1704
NmeAIII	GCCGAG	1 cut(s)	241
NmuCI	GTSAC	2 cut(s)	826, 912
NspI	RCATGY	1 cut(s)	445
PaeR7I	CTCGAG	1 cut(s)	335
PaqCI	CACCTGC	1 cut(s)	800
PcsI	WCGNNNNNNNCGW	1 cut(s)	382
PctI	GAATGC	1 cut(s)	879
PdmI	GAANNNNTTC	2 cut(s)	1241, 1278
PfeI	GAWTC	3 cut(s)	488, 838, 1206
PflMI	CCANNNNNTGG	1 cut(s)	355
PfoI	TCCNGGA	1 cut(s)	747
PpuMI	RGGWCCY	1 cut(s)	168
Psp1406I	AACGTT	1 cut(s)	1348
Psp5II	RGGWCCY	1 cut(s)	168
Psp6I	CCWGG	4 cut(s)	342, 747, 1050, 1705
PspGI	CCWGG	4 cut(s)	342, 747, 1050, 1705
PspN4I	GGNNCC	6 cut(s)	62, 322, 1012, 1049, 1476, 1704
PspPI	GGNCC	5 cut(s)	168, 252, 321, 346, 1010
PspPPI	RGGWCCY	1 cut(s)	168
PspXI	VCTCGAGB	1 cut(s)	335
PstNI	CAGNNNCTG	1 cut(s)	467
RsaI	GTAC	3 cut(s)	178, 379, 1650
RsaNI	GTAC	3 cut(s)	177, 378, 1649
RseI	CAYNNNNRTG	1 cut(s)	517
SaqAI	TTAA	4 cut(s)	417, 1284, 1355, 1553
Sau3AI	GATC	7 cut(s)	244, 478, 551, 607, 1093, 1410, 1537
Sau96I	GGNCC	5 cut(s)	168, 252, 321, 346, 1010
ScrFI	CCNGG	8 cut(s)	344, 749, 1052, 1480, 1481, 1699, 1700, 1707
SduI	GDGCHC	3 cut(s)	336, 461, 1705
SexAI	ACCWGGT	1 cut(s)	1705
SfaNI	GCATC	4 cut(s)	454, 521, 1255, 1367
Sfr274I	CTCGAG	1 cut(s)	335
SinI	GGWCC	3 cut(s)	168, 252, 321
SlaI	CTCGAG	1 cut(s)	335
SmaI	CCCGGG	2 cut(s)	1481, 1700
SmiMI	CAYNNNNRTG	1 cut(s)	517
SmlI	CTYRAG	2 cut(s)	296, 335
SmoI	CTYRAG	2 cut(s)	296, 335
SspI	AATATT	1 cut(s)	1529
SspMI	CTAG	3 cut(s)	849, 902, 1044
StyD4I	CCNGG	8 cut(s)	342, 747, 1050, 1478, 1479, 1697, 1698, 1705
StyI	CCWWGG	1 cut(s)	547
TaaI	ACNGT	4 cut(s)	949, 1453, 1604, 1653
TaiI	ACGT	1 cut(s)	1351
TaqI	TCGA	4 cut(s)	336, 1155, 1216, 1678
TatI	WGTACW	1 cut(s)	1648
TauI	GCSGC	5 cut(s)	99, 149, 152, 264, 1432
TfiI	GAWTC	3 cut(s)	488, 838, 1206
Tru1I	TTAA	4 cut(s)	417, 1284, 1355, 1553
Tru9I	TTAA	4 cut(s)	417, 1284, 1355, 1553
TseFI	GTSAC	2 cut(s)	826, 912
TseI	GCWGC	5 cut(s)	5, 8, 191, 212, 1457
Tsp45I	GTSAC	2 cut(s)	826, 912
TspDTI	ATGAA	3 cut(s)	397, 1359, 1563
TspGWI	ACGGA	1 cut(s)	1680
TspMI	CCCGGG	2 cut(s)	1479, 1698
Van91I	CCANNNNNTGG	1 cut(s)	355
VpaK11BI	GGWCC	3 cut(s)	168, 252, 321
XagI	CCTNNNNNAGG	1 cut(s)	1617
XapI	RAATTY	7 cut(s)	281, 647, 1030, 1058, 1178, 1298, 1304
XbaI	TCTAGA	1 cut(s)	848
XceI	RCATGY	1 cut(s)	445
XhoI	CTCGAG	1 cut(s)	335
XmaI	CCCGGG	2 cut(s)	1479, 1698
XmnI	GAANNNNTTC	2 cut(s)	1241, 1278
XspI	CTAG	3 cut(s)	849, 902, 1044

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.