Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0014367

Physical Location & Seq

Genomic Coordinates Reverse (-)

53133605 .. 53140615

Gene Structure 7011 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00120780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGCCGCCGCCGCCGCCTTTGCGCCACCCTCTTCTTCTCTCCGCCTCCGCTTCCTCTCCTTCTACCCCATCAGACCAATTCTCCCCCACTCCAAACCTCCAATTTTCCCGCCATTTCCTCCCCGGCCCTTCCCGCCCTCTGCCCCTCCACGCCGCTCCTTCTGCACCGTCGTCGCCGCAGCTCTCCACTCCGGAGGCGCCGCCGAGAGGACCAAGCCCGAAATCTCGGAAAAGTGGAGGGACAGAGTTGGCGAGTTCAGGAAGAAGCTCAAGGTCGCTGATATCAAGGGCGGCCCTGATGAAGGGCTCGACCGGCTGGGCCAGACTCTGGTGGTCACCGGCTGGGTTCGGACTCTTCGGGTCCAAAGCAGTGTCACCTTCATTGAGGTTAATGATGGGTCATGCCTCTCGAATATGCAATGTGTAATGGGTTCCGATGCGGAAGGCTATGATCAGGTAGAATCTGGCTCAATCACAACTGGTGCTTCGGTATGGGTGCAAGGCGTTGTAGCTACTAGCCAAGGATCAAAGCAAAAAGTGGAATTGAAGGTCAAGAAAATTGTTATGATTGGAAAGAGTGATCCTTCTTATCCTATCCAAAAGAAAAGGGTCAGCAGAGAATTTCTGAGAACGAAAGCTCATCTTCGGCCAAGAACAAATACTTTTGGTGCGGTTGCAAGAGTGAGAAATGCTTTGGCTTATGCTACTCACAACTTTTTCCAAGAAAATGGATTTGTGTGGGTCTCAAGTCCCATTATTACTGGTTCAGATTGTGAAGGAGCAGGTGAACAATTCTGTGTAACTACTCTGATTCCAAGCTCCAATGAAGTCTCTGATTCTCCTATCGATGGTATACCAAAAAAGGAGGATGGCCGAGTTGATTGGTCACAAGATTTCTTTGGGAAACCAGCATTCTTAACTGTCTCTGGCCAACTCAATGCTGAAACATATGCAACTGCTCTTTCTGATGTTTATACATTTGGTCCCACATTCAGGGCCGAAAATTCAAACACATCAAGACACCTTGCTGAATTCTGGATGATTGAGCCTGAGCTTGCATTTGCTGATCTGAATGATGACATGGCCTGTGCAACTGCCTATCTCCAGTATGTAGTGAGACATGTCCTTGATAATTGCAAAGAAGACATGGATTTTTTTAATACTTGGATTGAGAAAGGAATCATCGATCGATTGACTAATGTAGCTGAGAAAGACTTTGTACAGTTGACGTATACTGATGCAGTTGAACTTCTTCTTAAAACAAAGAAGAAATTTGAATTCCCAGTGAAGTGGGGATGTGATTTGCAGAGTGAGCATGAGTGTTATTTAACTGAAGAGGCATTTAGTGGATGCCCAGTAATTGTCAGGGACTATCCGAAGGAGATAAAAGCATTCTATATGCGGCAAAATGATGATGGAAAGACTGTGGCAGCTATGGATATGTTGGTTCCCCGGGTTGGAGAGCTTATCGGTGGAAGCCAGAGAGAAGAGAGGCTTGAATATTTAGAAGATCGGTTGGATGAATTAAAACTGAATAAGGAGGCATACTGGTGGTATCTTGATTTGCGTCGTTATGGGTCAGTTCCTCATGCAGGCTTTGGTCTTGGTTTCGAAAGGCTTGTTCAATTTGCCACTGGGATAGAGAATATCAGAGATGTGATTCCTTTCCCTCGGACACCTGGATCATCAGAATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004816 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006421 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

565

Amino Acids

63.31

Weight (kDa)

6.03

Isoelectric Point (pI)

43.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	207 - 559	1e-59	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014367)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17300 AT4G17300
fragaria_vesca	FvH4_1g25310
malus_domestica	MD06G1059300.v1.1
prunus_persica	Prupe.5G070100_v2.0.a1 Prupe.5G070100_v2.0.a1
pyrus_communis	pycom06g04930
rosa_chinensis	RchiOBHm_Chr2g0123321
rosa_multiflora	Rmu_sc0001917.1_g000001 Rmu_sc0021262.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120780
rosa_rugosa	Rorug02G0244500
rosa_samantha	Rh2AG303600 Rh2BG311700 Rh2CG290200 Rh2DG327600
rosa_wichuraiana	Rw2G024300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	773
Acc36I	ACCTGC	1 cut(s)	773
AccB1I	GGYRCC	1 cut(s)	197
AccB7I	CCANNNNNTGG	1 cut(s)	328
AccBSI	CCGCTC	1 cut(s)	156
AccI	GTMKAC	2 cut(s)	853, 1232
AccIII	TCCGGA	1 cut(s)	191
AclWI	GGATC	3 cut(s)	532, 575, 1690
AcoI	YGGCCR	4 cut(s)	3, 647, 871, 928
AcsI	RAATTY	6 cut(s)	620, 1003, 1031, 1271, 1277, 1691
AcuI	CTGAAG	1 cut(s)	1353
AcyI	GRCGYC	1 cut(s)	198
AfaI	GTAC	1 cut(s)	1221
AfiI	CCNNNNNNNGG	7 cut(s)	207, 302, 328, 848, 993, 1457, 1592
AflIII	ACRYGT	1 cut(s)	1120
AgsI	TTSAA	6 cut(s)	547, 1008, 1247, 1277, 1499, 1625
AjnI	CCWGG	1 cut(s)	1678
AluBI	AGCT	9 cut(s)	182, 268, 512, 638, 819, 1054, 1205, 1433, 1465
AluI	AGCT	9 cut(s)	182, 268, 512, 638, 819, 1054, 1205, 1433, 1465
Alw26I	GTCTC	4 cut(s)	748, 835, 928, 1111
AlwI	GGATC	3 cut(s)	532, 575, 1690
AlwNI	CAGNNNCTG	1 cut(s)	328
Ama87I	CYCGRG	1 cut(s)	1452
Aor13HI	TCCGGA	1 cut(s)	191
AoxI	GGCC	9 cut(s)	3, 125, 292, 319, 647, 871, 928, 996, 1083
ApeKI	GCWGC	2 cut(s)	179, 1430
ApoI	RAATTY	6 cut(s)	620, 1003, 1031, 1271, 1277, 1691
Asp700I	GAANNNNTTC	1 cut(s)	1251
AspLEI	GCGC	2 cut(s)	25, 200
AspS9I	GGNCC	7 cut(s)	126, 210, 293, 319, 361, 983, 996
AsuC2I	CCSGG	3 cut(s)	124, 1453, 1454
AsuHPI	GGTGA	3 cut(s)	328, 367, 797
AsuII	TTCGAA	1 cut(s)	1611
AvaI	CYCGRG	1 cut(s)	1452
AvaII	GGWCC	3 cut(s)	210, 361, 983
BalI	TGGCCA	1 cut(s)	930
BanI	GGYRCC	1 cut(s)	197
BanII	GRGCYC	1 cut(s)	309
BbsI	GAAGAC	1 cut(s)	1149
BbvI	GCAGC	2 cut(s)	191, 1442
BccI	CCATC	5 cut(s)	77, 389, 842, 863, 1409
BcgI	CGANNNNNNTGC	2 cut(s)	154, 188
BciT130I	CCWGG	1 cut(s)	1680
BclI	TGATCA	1 cut(s)	451
BcnI	CCSGG	3 cut(s)	124, 1453, 1454
BcoDI	GTCTC	4 cut(s)	748, 835, 928, 1111
BfaI	CTAG	1 cut(s)	516
BfoI	RGCGCY	1 cut(s)	201
BfuAI	ACCTGC	1 cut(s)	773
Bme1390I	CCNGG	4 cut(s)	124, 1453, 1454, 1680
Bme18I	GGWCC	3 cut(s)	210, 361, 983
BmeT110I	CYCGRG	1 cut(s)	1452
BmgT120I	GGNCC	7 cut(s)	126, 210, 293, 319, 361, 983, 996
BmiI	GGNNCC	5 cut(s)	199, 362, 433, 985, 1449
BmrFI	CCNGG	4 cut(s)	124, 1453, 1454, 1680
BmrI	ACTGGG	3 cut(s)	1277, 1349, 1644
BmsI	GCATC	3 cut(s)	427, 1228, 1340
BmuI	ACTGGG	3 cut(s)	1277, 1349, 1644
BpiI	GAAGAC	1 cut(s)	1149
BpmI	CTGGAG	1 cut(s)	1088
Bpu10I	CCTNAGC	1 cut(s)	1050
Bpu14I	TTCGAA	1 cut(s)	1611
BpuEI	CTTGAG	2 cut(s)	254, 730
BpuMI	CCSGG	3 cut(s)	124, 1453, 1454
Bsa29I	ATCGAT	3 cut(s)	846, 1185, 1189
BsaHI	GRCGYC	1 cut(s)	198
BsaI	GGTCTC	1 cut(s)	748
BsaJI	CCNNGG	5 cut(s)	122, 520, 1451, 1452, 1670
BsaWI	WCCGGW	1 cut(s)	191
BsaXI	ACNNNNNCTCC	2 cut(s)	66, 96
Bsc4I	CCNNNNNNNGG	7 cut(s)	207, 302, 328, 848, 993, 1457, 1592
Bse118I	RCCGGY	2 cut(s)	312, 338
Bse1I	ACTGG	7 cut(s)	484, 766, 1105, 1283, 1355, 1553, 1639
Bse3DI	GCAATG	1 cut(s)	425
BseAI	TCCGGA	1 cut(s)	191
BseBI	CCWGG	1 cut(s)	1680
BseCI	ATCGAT	3 cut(s)	846, 1185, 1189
BseDI	CCNNGG	5 cut(s)	122, 520, 1451, 1452, 1670
BseGI	GGATG	5 cut(s)	874, 1044, 1301, 1355, 1525
BseLI	CCNNNNNNNGG	7 cut(s)	207, 302, 328, 848, 993, 1457, 1592
BseMI	GCAATG	1 cut(s)	425
BseMII	CTCAG	3 cut(s)	617, 1041, 1197
BseNI	ACTGG	7 cut(s)	484, 766, 1105, 1283, 1355, 1553, 1639
BseXI	GCAGC	2 cut(s)	191, 1442
BseYI	CCCAGC	2 cut(s)	316, 342
BsgI	GTGCAG	1 cut(s)	148
Bsh1285I	CGRYCG	2 cut(s)	313, 1189
BshFI	GGCC	9 cut(s)	5, 127, 294, 321, 649, 873, 930, 998, 1085
BshNI	GGYRCC	1 cut(s)	197
BshVI	ATCGAT	3 cut(s)	846, 1185, 1189
BsiEI	CGRYCG	2 cut(s)	313, 1189
BsiHKCI	CYCGRG	1 cut(s)	1452
BsiSI	CCGG	5 cut(s)	124, 192, 313, 339, 1453
BslFI	GGGAC	4 cut(s)	254, 735, 969, 1382
BslI	CCNNNNNNNGG	7 cut(s)	207, 302, 328, 848, 993, 1457, 1592
BsmAI	GTCTC	4 cut(s)	748, 835, 928, 1111
BsmFI	GGGAC	4 cut(s)	254, 735, 969, 1382
BsmI	GAATGC	2 cut(s)	911, 1391
BsnI	GGCC	9 cut(s)	5, 127, 294, 321, 649, 873, 930, 998, 1085
Bso31I	GGTCTC	1 cut(s)	748
BsoBI	CYCGRG	1 cut(s)	1452
Bsp119I	TTCGAA	1 cut(s)	1611
Bsp1286I	GDGCHC	1 cut(s)	309
Bsp13I	TCCGGA	1 cut(s)	191
Bsp1407I	TGTACA	1 cut(s)	1219
Bsp143I	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
BspANI	GGCC	9 cut(s)	5, 127, 294, 321, 649, 873, 930, 998, 1085
BspCNI	CTCAG	3 cut(s)	618, 1042, 1198
BspDI	ATCGAT	3 cut(s)	846, 1185, 1189
BspEI	TCCGGA	1 cut(s)	191
BspLI	GGNNCC	5 cut(s)	199, 362, 433, 985, 1449
BspMI	ACCTGC	1 cut(s)	773
BspPI	GGATC	3 cut(s)	532, 575, 1690
BspT104I	TTCGAA	1 cut(s)	1611
BspT107I	GGYRCC	1 cut(s)	197
BspTNI	GGTCTC	1 cut(s)	748
BsrBI	CCGCTC	1 cut(s)	156
BsrDI	GCAATG	1 cut(s)	425
BsrFI	RCCGGY	2 cut(s)	312, 338
BsrGI	TGTACA	1 cut(s)	1219
BsrI	ACTGG	7 cut(s)	484, 766, 1105, 1283, 1355, 1553, 1639
BssAI	RCCGGY	2 cut(s)	312, 338
BssECI	CCNNGG	5 cut(s)	122, 520, 1451, 1452, 1670
BssMI	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
BssNAI	GTATAC	2 cut(s)	854, 1233
BssNI	GRCGYC	1 cut(s)	198
BssT1I	CCWWGG	1 cut(s)	520
Bst1107I	GTATAC	2 cut(s)	854, 1233
Bst2UI	CCWGG	1 cut(s)	1680
Bst4CI	ACNGT	4 cut(s)	169, 922, 1224, 1426
Bst6I	CTCTTC	4 cut(s)	37, 360, 1329, 1482
BstACI	GRCGYC	1 cut(s)	198
BstAUI	TGTACA	1 cut(s)	1219
BstBI	TTCGAA	1 cut(s)	1611
BstC8I	GCNNGC	2 cut(s)	1056, 1594
BstDEI	CTNAG	3 cut(s)	626, 1050, 1206
BstEII	GGTNACC	1 cut(s)	334
BstENI	CCTNNNNNAGG	2 cut(s)	300, 1590
BstF5I	GGATG	5 cut(s)	874, 1044, 1301, 1355, 1525
BstH2I	RGCGCY	1 cut(s)	201
BstHHI	GCGC	2 cut(s)	25, 200
BstKTI	GATC	7 cut(s)	454, 527, 583, 1069, 1189, 1513, 1685
BstMAI	GTCTC	4 cut(s)	748, 835, 928, 1111
BstMBI	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
BstMCI	CGRYCG	2 cut(s)	313, 1189
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 14, 20, 133, 162, 313, 1312
BstNI	CCWGG	1 cut(s)	1680
BstNSI	RCATGY	1 cut(s)	1124
BstPI	GGTNACC	1 cut(s)	334
BstSCI	CCNGG	4 cut(s)	122, 1451, 1452, 1678
BstV1I	GCAGC	2 cut(s)	191, 1442
BstV2I	GAAGAC	1 cut(s)	1149
BstXI	CCANNNNNNTGG	2 cut(s)	728, 1290
BstZ17I	GTATAC	2 cut(s)	854, 1233
Bsu15I	ATCGAT	3 cut(s)	846, 1185, 1189
BsuRI	GGCC	9 cut(s)	5, 127, 294, 321, 649, 873, 930, 998, 1085
BsuTUI	ATCGAT	3 cut(s)	846, 1185, 1189
BtsCI	GGATG	5 cut(s)	874, 1044, 1301, 1355, 1525
BtsI	GCAGTG	1 cut(s)	376
BtsIMutI	CAGTG	3 cut(s)	376, 1290, 1632
BveI	ACCTGC	1 cut(s)	773
Cac8I	GCNNGC	2 cut(s)	1056, 1594
CaiI	CAGNNNCTG	1 cut(s)	328
CfoI	GCGC	2 cut(s)	25, 200
Cfr10I	RCCGGY	2 cut(s)	312, 338
Cfr13I	GGNCC	7 cut(s)	126, 210, 293, 319, 361, 983, 996
Cfr9I	CCCGGG	1 cut(s)	1452
ClaI	ATCGAT	3 cut(s)	846, 1185, 1189
CseI	GACGC	1 cut(s)	1556
Csp6I	GTAC	1 cut(s)	1220
CviAII	CATG	6 cut(s)	402, 1081, 1121, 1147, 1316, 1589
CviQI	GTAC	1 cut(s)	1220
DdeI	CTNAG	3 cut(s)	626, 1050, 1206
DinI	GGCGCC	1 cut(s)	199
DpnI	GATC	7 cut(s)	453, 526, 582, 1068, 1188, 1512, 1684
DpnII	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
EaeI	YGGCCR	4 cut(s)	3, 647, 871, 928
Eam1104I	CTCTTC	4 cut(s)	37, 360, 1329, 1482
EarI	CTCTTC	4 cut(s)	37, 360, 1329, 1482
EciI	GGCGGA	1 cut(s)	32
Eco130I	CCWWGG	1 cut(s)	520
Eco24I	GRGCYC	1 cut(s)	309
Eco31I	GGTCTC	1 cut(s)	748
Eco32I	GATATC	1 cut(s)	283
Eco47I	GGWCC	3 cut(s)	210, 361, 983
Eco57I	CTGAAG	1 cut(s)	1353
Eco88I	CYCGRG	1 cut(s)	1452
Eco91I	GGTNACC	1 cut(s)	334
EcoNI	CCTNNNNNAGG	2 cut(s)	300, 1590
EcoO65I	GGTNACC	1 cut(s)	334
EcoRI	GAATTC	2 cut(s)	1031, 1277
EcoRII	CCWGG	1 cut(s)	1678
EcoRV	GATATC	1 cut(s)	283
EcoT14I	CCWWGG	1 cut(s)	520
EcoT38I	GRGCYC	1 cut(s)	309
EgeI	GGCGCC	1 cut(s)	199
EheI	GGCGCC	1 cut(s)	199
ErhI	CCWWGG	1 cut(s)	520
FaeI	CATG	6 cut(s)	405, 1084, 1124, 1150, 1319, 1592
FalI	AAGNNNNNCTT	4 cut(s)	627, 659, 1479, 1511
FaqI	GGGAC	4 cut(s)	254, 735, 969, 1382
FatI	CATG	6 cut(s)	401, 1080, 1120, 1146, 1315, 1588
FauI	CCCGC	2 cut(s)	117, 141
FauNDI	CATATG	1 cut(s)	949
FbaI	TGATCA	1 cut(s)	451
FblI	GTMKAC	2 cut(s)	853, 1232
FokI	GGATG	5 cut(s)	881, 1051, 1308, 1362, 1532
FriOI	GRGCYC	1 cut(s)	309
FspBI	CTAG	1 cut(s)	516
GlaI	GCGC	2 cut(s)	24, 199
GsaI	CCCAGC	2 cut(s)	320, 346
GsuI	CTGGAG	1 cut(s)	1088
HaeII	RGCGCY	1 cut(s)	201
HaeIII	GGCC	9 cut(s)	5, 127, 294, 321, 649, 873, 930, 998, 1085
HapII	CCGG	5 cut(s)	124, 192, 313, 339, 1453
HgaI	GACGC	1 cut(s)	1556
HhaI	GCGC	2 cut(s)	25, 200
Hin1I	GRCGYC	1 cut(s)	198
Hin1II	CATG	6 cut(s)	405, 1084, 1124, 1150, 1319, 1592
Hin6I	GCGC	2 cut(s)	23, 198
HinP1I	GCGC	2 cut(s)	23, 198
HincII	GTYRAC	1 cut(s)	1227
HindII	GTYRAC	1 cut(s)	1227
HinfI	GANTC	7 cut(s)	325, 352, 461, 811, 836, 1179, 1660
HpaII	CCGG	5 cut(s)	124, 192, 313, 339, 1453
HphI	GGTGA	3 cut(s)	328, 367, 797
Hpy166II	GTNNAC	4 cut(s)	788, 854, 1227, 1233
Hpy188III	TCNNGA	7 cut(s)	192, 259, 409, 553, 1017, 1036, 1559
Hpy8I	GTNNAC	4 cut(s)	788, 854, 1227, 1233
Hpy99I	CGWCG	3 cut(s)	173, 176, 1572
HpyCH4III	ACNGT	4 cut(s)	169, 922, 1224, 1426
HpyCH4IV	ACGT	1 cut(s)	1229
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 14, 20, 133, 162, 313, 1312
HpyF3I	CTNAG	3 cut(s)	626, 1050, 1206
HpySE526I	ACGT	1 cut(s)	1229
Hsp92I	GRCGYC	1 cut(s)	198
Hsp92II	CATG	6 cut(s)	405, 1084, 1124, 1150, 1319, 1592
HspAI	GCGC	2 cut(s)	23, 198
KasI	GGCGCC	1 cut(s)	197
Kpn2I	TCCGGA	1 cut(s)	191
Ksp22I	TGATCA	1 cut(s)	451
Kzo9I	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
LmnI	GCTCC	3 cut(s)	161, 779, 824
Lsp1109I	GCAGC	2 cut(s)	191, 1442
LweI	GCATC	3 cut(s)	427, 1228, 1340
MaeI	CTAG	1 cut(s)	516
MaeII	ACGT	1 cut(s)	1229
MaeIII	GTNAC	4 cut(s)	334, 373, 799, 885
MalI	GATC	7 cut(s)	453, 526, 582, 1068, 1188, 1512, 1684
MbiI	CCGCTC	1 cut(s)	156
MboI	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
MhlI	GDGCHC	1 cut(s)	309
MlsI	TGGCCA	1 cut(s)	930
MluNI	TGGCCA	1 cut(s)	930
Mly113I	GGCGCC	1 cut(s)	198
MlyI	GAGTC	2 cut(s)	319, 346
MmeI	TCCRAC	2 cut(s)	1438, 1497
Mox20I	TGGCCA	1 cut(s)	930
MroI	TCCGGA	1 cut(s)	191
MroXI	GAANNNNTTC	1 cut(s)	1251
MscI	TGGCCA	1 cut(s)	930
MseI	TTAA	7 cut(s)	390, 917, 1158, 1257, 1328, 1526, 1696
MslI	CAYNNNNRTG	1 cut(s)	1549
Msp20I	TGGCCA	1 cut(s)	930
MspI	CCGG	5 cut(s)	124, 192, 313, 339, 1453
MspR9I	CCNGG	4 cut(s)	124, 1453, 1454, 1680
Mva1269I	GAATGC	2 cut(s)	911, 1391
MvaI	CCWGG	1 cut(s)	1680
MwoI	GCNNNNNNNGC	7 cut(s)	11, 14, 20, 133, 162, 313, 1312
NarI	GGCGCC	1 cut(s)	198
NciI	CCSGG	3 cut(s)	124, 1453, 1454
NdeI	CATATG	1 cut(s)	949
NdeII	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
NlaIII	CATG	6 cut(s)	405, 1084, 1124, 1150, 1319, 1592
NlaIV	GGNNCC	5 cut(s)	199, 362, 433, 985, 1449
NmeAIII	GCCGAG	2 cut(s)	229, 899
NmuCI	GTSAC	3 cut(s)	334, 373, 885
NspI	RCATGY	1 cut(s)	1124
NspV	TTCGAA	1 cut(s)	1611
PaqCI	CACCTGC	1 cut(s)	773
PciI	ACATGT	1 cut(s)	1120
PctI	GAATGC	2 cut(s)	911, 1391
PdmI	GAANNNNTTC	1 cut(s)	1251
PfeI	GAWTC	5 cut(s)	461, 811, 836, 1179, 1660
PflMI	CCANNNNNTGG	1 cut(s)	328
Ple19I	CGATCG	1 cut(s)	1189
PleI	GAGTC	2 cut(s)	319, 346
PluTI	GGCGCC	1 cut(s)	201
PpsI	GAGTC	2 cut(s)	319, 346
PscI	ACATGT	1 cut(s)	1120
Psp6I	CCWGG	1 cut(s)	1678
PspEI	GGTNACC	1 cut(s)	334
PspFI	CCCAGC	2 cut(s)	316, 342
PspGI	CCWGG	1 cut(s)	1678
PspN4I	GGNNCC	5 cut(s)	199, 362, 433, 985, 1449
PspPI	GGNCC	7 cut(s)	126, 210, 293, 319, 361, 983, 996
PstNI	CAGNNNCTG	1 cut(s)	328
PvuI	CGATCG	1 cut(s)	1189
RsaI	GTAC	1 cut(s)	1221
RsaNI	GTAC	1 cut(s)	1220
RseI	CAYNNNNRTG	1 cut(s)	1549
SaqAI	TTAA	7 cut(s)	390, 917, 1158, 1257, 1328, 1526, 1696
Sau3AI	GATC	7 cut(s)	451, 524, 580, 1066, 1186, 1510, 1682
Sau96I	GGNCC	7 cut(s)	126, 210, 293, 319, 361, 983, 996
SchI	GAGTC	2 cut(s)	319, 346
ScrFI	CCNGG	4 cut(s)	124, 1453, 1454, 1680
SduI	GDGCHC	1 cut(s)	309
SfaNI	GCATC	3 cut(s)	427, 1228, 1340
SfoI	GGCGCC	1 cut(s)	199
SfuI	TTCGAA	1 cut(s)	1611
SinI	GGWCC	3 cut(s)	210, 361, 983
SmaI	CCCGGG	1 cut(s)	1454
SmiMI	CAYNNNNRTG	1 cut(s)	1549
SmlI	CTYRAG	2 cut(s)	269, 745
SmoI	CTYRAG	2 cut(s)	269, 745
SspDI	GGCGCC	1 cut(s)	197
SspI	AATATT	1 cut(s)	1502
SspMI	CTAG	1 cut(s)	516
StyD4I	CCNGG	4 cut(s)	122, 1451, 1452, 1678
StyI	CCWWGG	1 cut(s)	520
TaaI	ACNGT	4 cut(s)	169, 922, 1224, 1426
TaiI	ACGT	1 cut(s)	1232
TaqI	TCGA	6 cut(s)	309, 410, 846, 1185, 1189, 1611
TatI	WGTACW	1 cut(s)	1219
TauI	GCSGC	9 cut(s)	8, 11, 14, 17, 156, 179, 203, 294, 1405
TfiI	GAWTC	5 cut(s)	461, 811, 836, 1179, 1660
Tru1I	TTAA	7 cut(s)	390, 917, 1158, 1257, 1328, 1526, 1696
Tru9I	TTAA	7 cut(s)	390, 917, 1158, 1257, 1328, 1526, 1696
TscAI	CASTG	3 cut(s)	376, 1290, 1639
TseFI	GTSAC	3 cut(s)	334, 373, 885
TseI	GCWGC	2 cut(s)	179, 1430
Tsp45I	GTSAC	3 cut(s)	334, 373, 885
TspDTI	ATGAA	4 cut(s)	315, 370, 840, 1536
TspMI	CCCGGG	1 cut(s)	1452
TspRI	CASTG	3 cut(s)	376, 1290, 1639
Van91I	CCANNNNNTGG	1 cut(s)	328
VpaK11BI	GGWCC	3 cut(s)	210, 361, 983
XagI	CCTNNNNNAGG	2 cut(s)	300, 1590
XapI	RAATTY	6 cut(s)	620, 1003, 1031, 1271, 1277, 1691
XceI	RCATGY	1 cut(s)	1124
XmaI	CCCGGG	1 cut(s)	1452
XmiI	GTMKAC	2 cut(s)	853, 1232
XmnI	GAANNNNTTC	1 cut(s)	1251
XspI	CTAG	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.