MD06G1086300.v1.1

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015618
Physical Location & Seq
Reverse (-)
21314990 .. 21317988
2999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1086300.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGAGTGGTGTTAGTGGAGACTCGAGAACTGGTAACACCGCTTTTGAGACTATAAACGCAGCTGCTTCCGCGATTGCAGCCGCGGAGAACCGTGCGCCTCACGCCACCGCTCAGAAGAGAAGATGGGGTAGTTTCCTGAGCACATATTGGTGTTTTGGATTTCAAAGACACAGAAAGAGCATTGGGCATATTGCTCCAGAACCGACGGACCCTGGAGGTGATGCTTCAAGAGCGGAAAGTTCAATCCAGGCACCTTCAATTGTATTTCCCTTTGTTGCACCTCCGTCCTCTCCTGCATCCTTTCTTCAATCAGAACCACCTTCAGTCACACAATCACCAGCTACTGGCTTTTTCTCTCTTTCAGCAAGCATGTACTCCCCTGGGCCTGCCTCAATTTTTGCTATCGGTCCTTATGCTCACGACACCCAGTTGGTCTCACCGCCTGTTTTCTCAACCCTCACTACTGAACCATCAACTGCTCCCTTTACTCCTCCTCCTGAGTCTGTTCATTTGACTACCCCTTCCTCGCCCGAGGTTCCATTTGCTCAGCTTCTTGATCCTCACTTTCGGAATGGTGAAGGTGGTCAGAGATTCCCATTATCTCACTACGAGTTTCCGTCTTACCAACTTTACCCTGGAAGCCCAGTTGGTCAGCTTATATCGCCAAGCTCAGCAATCTCAGGTTCTGGTACTTCGTCTCCTTTTCCCGACCGCGACTTTGCTGTTGGTGGTCCTCACTTCCTGGAATTCCGAACAGGTGATCCTCCTAAGCTCTTGAACCTTGACATTCTTTCGACCCGTGATTGGGGTTCAAGACTAGCTTCTGGTTCTGTGACCCCTGATGGTGCAAGGTCAACAGTTTCTGATGGTTTCCTTCAAAAGCCTCAAACCCCTGAGGTTGTATTAAATCCACGATCCAACACTAGAGGTAGAAATAATGGAATTGTGATCAACCATAGAGTTTCCTTTGAGTTAAGTTCTGAAGCAATTGTAAGAAGTGTAGAAAAAAAGACGGCATTAGCGGAAGCTGCATCAACTTCTATAGAAGAAACAGAAAAGGGCCAGAGCGAAGAGGATCCTTGCAAAGCAGTGAGCAGTTCCGAGTGCCCTGCCTGTGAGACACCCAGTGATGCACCAGAGAACGCTGTGGCCGATGAGGAGGATGCACAGCTGCACCAGAAGCACCGATCCATCACACTTGGTTCTAGTAAGGAATTCAATTTTGACAATGCTGATGGAGGAGATTCAGGTAACTCAAATACTATTGGCTCCGACTGGTGGGCAAATGAGAAAGTTAGTCTGAAGGAAAACGGGACACCAAAGAATTGGTCTTTCTTCCCTATGGTGCAGCCGGGTGTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

48.28

Weight (kDa)

5.14

Isoelectric Point (pI)

60.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02720
malus_domestica MD06G1086300.v1.1 MD14G1107500.v1.1
prunus_persica Prupe.5G102900_v2.0.a1
pyrus_communis pycom06g08290 pycom14g10250
rosa_chinensis RchiOBHm_Chr7g0197211
rosa_laevigata RLG00000003951
rosa_multiflora Rmu_ssc0000465.1_g000014
rosa_roxburghii Rroxscaffold_3G00258590
rosa_rugosa Rorug07G0038700
rosa_samantha Rh7AG163300 Rh7BG165300 Rh7CG171300 Rh7DG164700
rosa_wichuraiana Rw7G014260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 344
AccBSI CCGCTC 2 cut(s) 110, 233
AccII CGCG 3 cut(s) 71, 83, 714
AciI CCGC 9 cut(s) 39, 69, 81, 83, 108, 233, 440, 712, 1022
AclWI GGATC 6 cut(s) 551, 755, 909, 1070, 1083, 1182
AcoI YGGCCR 1 cut(s) 1149
AcsI RAATTY 2 cut(s) 746, 1214
AcuI CTGAAG 3 cut(s) 306, 1002, 1322
AdeI CACNNNGTG 1 cut(s) 1127
AfaI GTAC 2 cut(s) 374, 691
AfiI CCNNNNNNNGG 4 cut(s) 344, 804, 805, 1278
AgsI TTSAA 9 cut(s) 164, 228, 243, 258, 308, 778, 813, 878, 1219
AjnI CCWGG 5 cut(s) 211, 246, 379, 634, 741
AloI GAACNNNNNNTCC 2 cut(s) 745, 777
AluBI AGCT 9 cut(s) 62, 341, 550, 655, 669, 772, 821, 1028, 1171
AluI AGCT 9 cut(s) 62, 341, 550, 655, 669, 772, 821, 1028, 1171
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 5 cut(s) 12, 41, 439, 702, 1112
AlwI GGATC 6 cut(s) 551, 755, 909, 1070, 1083, 1182
AlwNI CAGNNNCTG 3 cut(s) 344, 686, 863
Ama87I CYCGRG 2 cut(s) 22, 530
AoxI GGCC 3 cut(s) 383, 1060, 1149
ApeKI GCWGC 6 cut(s) 59, 62, 77, 1028, 1171, 1348
ApoI RAATTY 2 cut(s) 746, 1214
ArsI GACNNNNNNTTYG 4 cut(s) 701, 733, 1313, 1345
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 5 cut(s) 208, 383, 407, 731, 1060
AsuC2I CCSGG 1 cut(s) 1353
AsuHPI GGTGA 5 cut(s) 230, 327, 429, 587, 770
AvaI CYCGRG 2 cut(s) 22, 530
AvaII GGWCC 3 cut(s) 208, 407, 731
AxyI CCTNAGG 1 cut(s) 894
BaeGI GKGCMC 1 cut(s) 1109
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 6 cut(s) 49, 71, 89, 1015, 1158, 1360
BccI CCATC 6 cut(s) 117, 478, 836, 860, 1199, 1229
BceAI ACGGC 1 cut(s) 1029
BcgI CGANNNNNNTGC 2 cut(s) 1091, 1125
BciT130I CCWGG 5 cut(s) 213, 248, 381, 636, 743
BclI TGATCA 1 cut(s) 948
BcnI CCSGG 1 cut(s) 1353
BcoDI GTCTC 5 cut(s) 12, 41, 439, 702, 1112
BfaI CTAG 3 cut(s) 818, 924, 1206
BfmI CTRYAG 1 cut(s) 1041
BisI GCNGC 7 cut(s) 60, 63, 78, 81, 1029, 1172, 1349
BlpI GCTNAGC 2 cut(s) 546, 670
BlsI GCNGC 7 cut(s) 61, 64, 79, 82, 1030, 1173, 1350
Bme1390I CCNGG 6 cut(s) 213, 248, 381, 636, 743, 1353
Bme18I GGWCC 3 cut(s) 208, 407, 731
BmeT110I CYCGRG 2 cut(s) 22, 530
BmgT120I GGNCC 5 cut(s) 208, 383, 407, 731, 1060
BmiI GGNNCC 5 cut(s) 210, 252, 537, 1077, 1270
BmrFI CCNGG 6 cut(s) 213, 248, 381, 636, 743, 1353
BmrI ACTGGG 3 cut(s) 421, 638, 1119
BmsI GCATC 5 cut(s) 211, 305, 1040, 1120, 1153
BmuI ACTGGG 3 cut(s) 421, 638, 1119
BpmI CTGGAG 2 cut(s) 180, 234
Bpu10I CCTNAGC 2 cut(s) 137, 768
Bpu1102I GCTNAGC 2 cut(s) 546, 670
BpuMI CCSGG 1 cut(s) 1353
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 6 cut(s) 81, 211, 379, 380, 531, 634
BsaXI ACNNNNNCTCC 4 cut(s) 478, 508, 682, 712
Bsc4I CCNNNNNNNGG 4 cut(s) 344, 804, 805, 1278
Bse1I ACTGG 6 cut(s) 34, 349, 427, 644, 1125, 1280
Bse21I CCTNAGG 1 cut(s) 894
BseBI CCWGG 5 cut(s) 213, 248, 381, 636, 743
BseDI CCNNGG 6 cut(s) 81, 211, 379, 380, 531, 634
BseGI GGATG 2 cut(s) 296, 1168
BseLI CCNNNNNNNGG 4 cut(s) 344, 804, 805, 1278
BseMII CTCAG 7 cut(s) 125, 128, 489, 560, 684, 693, 885
BseNI ACTGG 6 cut(s) 34, 349, 427, 644, 1125, 1280
BseRI GAGGAG 4 cut(s) 480, 483, 1172, 1254
BseSI GKGCMC 1 cut(s) 1109
BseXI GCAGC 6 cut(s) 49, 71, 89, 1015, 1158, 1360
BsgI GTGCAG 1 cut(s) 1157
Bsh1236I CGCG 3 cut(s) 71, 83, 714
Bsh1285I CGRYCG 1 cut(s) 712
BshFI GGCC 3 cut(s) 385, 1062, 1151
BshNI GGYRCC 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 712
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 2 cut(s) 22, 530
BsiSI CCGG 1 cut(s) 1352
BslFI GGGAC 1 cut(s) 1327
BslI CCNNNNNNNGG 4 cut(s) 344, 804, 805, 1278
BsmAI GTCTC 5 cut(s) 12, 41, 439, 702, 1112
BsmBI CGTCTC 1 cut(s) 702
BsmFI GGGAC 1 cut(s) 1327
BsnI GGCC 3 cut(s) 385, 1062, 1151
Bso31I GGTCTC 1 cut(s) 439
BsoBI CYCGRG 2 cut(s) 22, 530
Bsp1286I GDGCHC 2 cut(s) 143, 1109
Bsp143I GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
Bsp1720I GCTNAGC 2 cut(s) 546, 670
BspACI CCGC 9 cut(s) 39, 69, 81, 83, 108, 233, 440, 712, 1022
BspANI GGCC 3 cut(s) 385, 1062, 1151
BspCNI CTCAG 7 cut(s) 124, 129, 490, 559, 683, 692, 886
BspFNI CGCG 3 cut(s) 71, 83, 714
BspLI GGNNCC 5 cut(s) 210, 252, 537, 1077, 1270
BspPI GGATC 6 cut(s) 551, 755, 909, 1070, 1083, 1182
BspT107I GGYRCC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 439
BsrBI CCGCTC 2 cut(s) 110, 233
BsrI ACTGG 6 cut(s) 34, 349, 427, 644, 1125, 1280
BssECI CCNNGG 6 cut(s) 81, 211, 379, 380, 531, 634
BssMI GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
Bst2UI CCWGG 5 cut(s) 213, 248, 381, 636, 743
Bst4CI ACNGT 2 cut(s) 92, 859
Bst6I CTCTTC 2 cut(s) 110, 1065
BstC8I GCNNGC 2 cut(s) 367, 387
BstDEI CTNAG 8 cut(s) 111, 137, 498, 546, 670, 679, 768, 894
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 296, 1168
BstFNI CGCG 3 cut(s) 71, 83, 714
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 6 cut(s) 559, 763, 917, 951, 1078, 1190
BstMAI GTCTC 5 cut(s) 12, 41, 439, 702, 1112
BstMBI GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
BstMCI CGRYCG 1 cut(s) 712
BstMWI GCNNNNNNNGC 7 cut(s) 68, 77, 101, 230, 661, 1028, 1180
BstNI CCWGG 5 cut(s) 213, 248, 381, 636, 743
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 6 cut(s) 211, 246, 379, 634, 741, 1351
BstSFI CTRYAG 1 cut(s) 1041
BstSLI GKGCMC 1 cut(s) 1109
BstUI CGCG 3 cut(s) 71, 83, 714
BstV1I GCAGC 6 cut(s) 49, 71, 89, 1015, 1158, 1360
BstX2I RGATCY 1 cut(s) 1075
BstXI CCANNNNNNTGG 1 cut(s) 1326
BstYI RGATCY 1 cut(s) 1075
Bsu36I CCTNAGG 1 cut(s) 894
BsuRI GGCC 3 cut(s) 385, 1062, 1151
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 296, 1168
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 2 cut(s) 1095, 1132
Cac8I GCNNGC 2 cut(s) 367, 387
CaiI CAGNNNCTG 3 cut(s) 344, 686, 863
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 5 cut(s) 208, 383, 407, 731, 1060
Cfr42I CCGCGG 1 cut(s) 84
Csp6I GTAC 2 cut(s) 373, 690
CviAII CATG 1 cut(s) 370
CviQI GTAC 2 cut(s) 373, 690
DdeI CTNAG 8 cut(s) 111, 137, 498, 546, 670, 679, 768, 894
DpnI GATC 6 cut(s) 558, 762, 916, 950, 1077, 1189
DpnII GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
DraIII CACNNNGTG 1 cut(s) 1127
EaeI YGGCCR 1 cut(s) 1149
Eam1104I CTCTTC 2 cut(s) 110, 1065
EarI CTCTTC 2 cut(s) 110, 1065
Eco31I GGTCTC 1 cut(s) 439
Eco47I GGWCC 3 cut(s) 208, 407, 731
Eco57I CTGAAG 3 cut(s) 306, 1002, 1322
Eco81I CCTNAGG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 22, 530
EcoRI GAATTC 2 cut(s) 746, 1214
EcoRII CCWGG 5 cut(s) 211, 246, 379, 634, 741
Esp3I CGTCTC 1 cut(s) 702
FaeI CATG 1 cut(s) 373
FaiI YATR 9 cut(s) 53, 145, 189, 371, 414, 659, 957, 1043, 1343
FalI AAGNNNNNCTT 2 cut(s) 805, 837
FaqI GGGAC 1 cut(s) 1327
FatI CATG 1 cut(s) 369
FbaI TGATCA 1 cut(s) 948
Fnu4HI GCNGC 7 cut(s) 60, 63, 78, 81, 1029, 1172, 1349
FokI GGATG 2 cut(s) 283, 1175
Fsp4HI GCNGC 7 cut(s) 60, 63, 78, 81, 1029, 1172, 1349
FspBI CTAG 3 cut(s) 818, 924, 1206
GlaI GCGC 1 cut(s) 96
GluI GCNGC 7 cut(s) 60, 63, 78, 81, 1029, 1172, 1349
GsuI CTGGAG 2 cut(s) 180, 234
HaeIII GGCC 3 cut(s) 385, 1062, 1151
HapII CCGG 1 cut(s) 1352
HhaI GCGC 1 cut(s) 97
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 4 cut(s) 20, 500, 591, 1244
HpaII CCGG 1 cut(s) 1352
HphI GGTGA 5 cut(s) 230, 327, 429, 587, 770
Hpy166II GTNNAC 1 cut(s) 855
Hpy8I GTNNAC 1 cut(s) 855
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 6 cut(s) 264, 330, 531, 572, 884, 1297
HpyCH4III ACNGT 2 cut(s) 92, 859
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 77, 101, 230, 661, 1028, 1180
HpyF3I CTNAG 8 cut(s) 111, 137, 498, 546, 670, 679, 768, 894
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 948
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
LmnI GCTCC 3 cut(s) 199, 484, 1274
Lsp1109I GCAGC 6 cut(s) 49, 71, 89, 1015, 1158, 1360
LweI GCATC 5 cut(s) 211, 305, 1040, 1120, 1153
MaeI CTAG 3 cut(s) 818, 924, 1206
MaeIII GTNAC 4 cut(s) 32, 325, 832, 1250
MalI GATC 6 cut(s) 558, 762, 916, 950, 1077, 1189
MbiI CCGCTC 2 cut(s) 110, 233
MboI GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
MboII GAAGA 6 cut(s) 127, 132, 296, 1058, 1082, 1327
MfeI CAATTG 2 cut(s) 258, 987
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 2 cut(s) 143, 1109
MluCI AATT 8 cut(s) 258, 393, 746, 942, 987, 1214, 1219, 1324
MlyI GAGTC 2 cut(s) 14, 509
MmeI TCCRAC 2 cut(s) 942, 1296
MseI TTAA 3 cut(s) 905, 974, 1363
MslI CAYNNNNRTG 1 cut(s) 148
MspA1I CMGCKG 3 cut(s) 62, 83, 1171
MspI CCGG 1 cut(s) 1352
MspR9I CCNGG 6 cut(s) 213, 248, 381, 636, 743, 1353
MunI CAATTG 2 cut(s) 258, 987
MvaI CCWGG 5 cut(s) 213, 248, 381, 636, 743
MvnI CGCG 3 cut(s) 71, 83, 714
MwoI GCNNNNNNNGC 7 cut(s) 68, 77, 101, 230, 661, 1028, 1180
NciI CCSGG 1 cut(s) 1353
NdeII GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 5 cut(s) 210, 252, 537, 1077, 1270
NmuCI GTSAC 2 cut(s) 325, 832
NspI RCATGY 1 cut(s) 373
PaeR7I CTCGAG 1 cut(s) 22
PasI CCCWGGG 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 1149
PfeI GAWTC 2 cut(s) 591, 1244
PflMI CCANNNNNTGG 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 7 cut(s) 61, 64, 79, 82, 1030, 1173, 1350
PleI GAGTC 2 cut(s) 14, 508
PpsI GAGTC 2 cut(s) 14, 508
Psp6I CCWGG 5 cut(s) 211, 246, 379, 634, 741
PspGI CCWGG 5 cut(s) 211, 246, 379, 634, 741
PspN4I GGNNCC 5 cut(s) 210, 252, 537, 1077, 1270
PspPI GGNCC 5 cut(s) 208, 383, 407, 731, 1060
PstNI CAGNNNCTG 3 cut(s) 344, 686, 863
PsuI RGATCY 1 cut(s) 1075
PvuII CAGCTG 2 cut(s) 62, 1171
RsaI GTAC 2 cut(s) 374, 691
RsaNI GTAC 2 cut(s) 373, 690
RseI CAYNNNNRTG 1 cut(s) 148
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 3 cut(s) 905, 974, 1363
SatI GCNGC 7 cut(s) 60, 63, 78, 81, 1029, 1172, 1349
Sau3AI GATC 6 cut(s) 556, 760, 914, 948, 1075, 1187
Sau96I GGNCC 5 cut(s) 208, 383, 407, 731, 1060
SchI GAGTC 2 cut(s) 14, 509
ScrFI CCNGG 6 cut(s) 213, 248, 381, 636, 743, 1353
SduI GDGCHC 2 cut(s) 143, 1109
SfaNI GCATC 5 cut(s) 211, 305, 1040, 1120, 1153
SfcI CTRYAG 1 cut(s) 1041
Sfr274I CTCGAG 1 cut(s) 22
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 3 cut(s) 208, 407, 731
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 8 cut(s) 258, 393, 746, 942, 987, 1214, 1219, 1324
SsiI CCGC 9 cut(s) 39, 69, 81, 83, 108, 233, 440, 712, 1022
SspMI CTAG 3 cut(s) 818, 924, 1206
StyD4I CCNGG 6 cut(s) 211, 246, 379, 634, 741, 1351
TaaI ACNGT 2 cut(s) 92, 859
TaqI TCGA 2 cut(s) 23, 794
TaqII GACCGA 1 cut(s) 395
TasI AATT 8 cut(s) 258, 393, 746, 942, 987, 1214, 1219, 1324
TatI WGTACW 1 cut(s) 372
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 591, 1244
Tru1I TTAA 3 cut(s) 905, 974, 1363
Tru9I TTAA 3 cut(s) 905, 974, 1363
TscAI CASTG 2 cut(s) 1095, 1132
TseFI GTSAC 2 cut(s) 325, 832
TseI GCWGC 6 cut(s) 59, 62, 77, 1028, 1171, 1348
Tsp45I GTSAC 2 cut(s) 325, 832
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 3 cut(s) 221, 273, 606
TspRI CASTG 2 cut(s) 1095, 1132
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 3 cut(s) 208, 407, 731
XapI RAATTY 2 cut(s) 746, 1214
XceI RCATGY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 632
XhoI CTCGAG 1 cut(s) 22
XspI CTAG 3 cut(s) 818, 924, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.