pycom06g08290

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015618
Physical Location & Seq
Reverse (-)
13404630 .. 13406910
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08290.1

Sequence Viewer

Length: 1368 bp
ATGAGTGGTATTAATGGAGACTCGAGAACTGGTAACACCGCTTTTGAGACTATAAACGCAGCTGCTTCCGCGATCGCAGCCGCGGAGAACCGTGCGCCTCACGCCACCGTTCAGAAGAGAAGGTGGGGTAGTTTCTTGAGCACATATTGGTGTTTTGGATTTCAAAGACACAGAAAGAGCATTGGGCATATTGCTCCAGAACCGACGGACCCTGGAGGTGATGCTTCAAGAGCGGAAAGTTCAATCCAGGCACCTTCAATTGTATTTCCCTTTGTTGCACCTCCATCCTCTCCTGCATCGTTTCTTCAATCAGAACCACCTTCGGTCACACAATCACCAGCTACTGGTTTTTTCTCTCTTTCAGCAAGCATGTACTCCCCTGGGCCTGCCTCAATTTTTGCTATCGGTCCTTATGCTCATGACACCCAGTTGGTCTCACCGCCTGTTTTCTCAACCCTCACTACCGAACCATCAACTGCTCCCTTTACTCCTCCTCCTGAGTCTGTTCATTTGACTACCCCTTCCTCGCCCGAGGTTCCATTTGCTCAGCTTCTCGATCCCCACTTCCAGAATGGTGAAGTTGGTCAGAGATTCCCATTATCTCACTACGAGTTTCCGTCTTACCAACTTTACCCTGGAAGCCCGGTTGGTCAGCTTATATCGCCAAGCTCAGGAATCTCAGGTTCTGGTACTTCGTCTCCTTTTCCCGACCGCGACTTTGCTGTTGGTGGTCCTCACTTCCTGGAATTCCGAACAGGTGATCCTCCTAAGCTCTTGAACCTTGACATTCTTTCCACCCGTGATTGGGGTTCAAGACTAGCTTCTGGTTCTGTGACCCCTGATGGTGGAAGGTCAACAGTTTCTGATGGTTTCCTTCAAAAGCCTCAAACCCCTGAGGTTGTATTAAATCCACGATCCAACACTAGAGGTAGAAATAATGGAATTGTGATCAACCATAGAGTTTCCTTTGAGTTAAGTTCTGAAGCAATTGTAAGAAGTGTAGAAAAAAAGACGGTGGCGTTAGCGGAAGCTGCATCAACTTCTATACAAGAAACAGAAAAGGGCCAGAGCGAAGAGGATCCTTCCAAAGCAGTGAGCAGTGCCGAGTGTCCTGCCTGTGAGACACTCAGTGATGCACCAGAGAAAGCTGTGGCTGACGAGGAGGATGCACAGCTGCACCAGAAGCACCGATCCATCACGCTTGGTTCTAGTAAGGAATTCAATTTTGACAATGCTGATGGAGGAGATTCAGGTAACTCGAATACTATTGGCTCCGACTGGTGGGCAAATGAGAAAGTTAGTCCGAAGGAAAACGGGACACCAAAGAATTGGTCTTTCTTCCCTATGGTGCAGCCGGGTGTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

456

Amino Acids

48.42

Weight (kDa)

5.2

Isoelectric Point (pI)

58.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02720
malus_domestica MD06G1086300.v1.1 MD14G1107500.v1.1
prunus_persica Prupe.5G102900_v2.0.a1
pyrus_communis pycom06g08290 pycom14g10250
rosa_chinensis RchiOBHm_Chr7g0197211
rosa_laevigata RLG00000003951
rosa_multiflora Rmu_ssc0000465.1_g000014
rosa_roxburghii Rroxscaffold_3G00258590
rosa_rugosa Rorug07G0038700
rosa_samantha Rh7AG163300 Rh7BG165300 Rh7CG171300 Rh7DG164700
rosa_wichuraiana Rw7G014260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 233
AccII CGCG 3 cut(s) 71, 83, 714
AciI CCGC 8 cut(s) 39, 69, 81, 83, 233, 440, 712, 1025
AclWI GGATC 6 cut(s) 551, 755, 909, 1073, 1086, 1185
AcsI RAATTY 2 cut(s) 746, 1217
AcuI CTGAAG 1 cut(s) 1002
AdeI CACNNNGTG 1 cut(s) 1130
AfaI GTAC 2 cut(s) 374, 691
AfiI CCNNNNNNNGG 6 cut(s) 344, 671, 804, 805, 845, 1281
AgsI TTSAA 9 cut(s) 164, 228, 243, 258, 308, 778, 813, 878, 1222
AjnI CCWGG 5 cut(s) 211, 246, 379, 634, 741
AloI GAACNNNNNNTCC 2 cut(s) 745, 777
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 5 cut(s) 12, 41, 439, 702, 1115
AlwI GGATC 6 cut(s) 551, 755, 909, 1073, 1086, 1185
AlwNI CAGNNNCTG 3 cut(s) 344, 686, 863
Ama87I CYCGRG 2 cut(s) 22, 530
AoxI GGCC 2 cut(s) 383, 1063
ApeKI GCWGC 6 cut(s) 59, 62, 77, 1031, 1174, 1351
ApoI RAATTY 2 cut(s) 746, 1217
ArsI GACNNNNNNTTYG 4 cut(s) 701, 733, 1316, 1348
AseI ATTAAT 1 cut(s) 12
AsiSI GCGATCGC 1 cut(s) 75
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 5 cut(s) 208, 383, 407, 731, 1063
AsuC2I CCSGG 2 cut(s) 644, 1356
AsuHPI GGTGA 5 cut(s) 230, 327, 429, 587, 770
AvaI CYCGRG 2 cut(s) 22, 530
AvaII GGWCC 3 cut(s) 208, 407, 731
AxyI CCTNAGG 1 cut(s) 894
BamHI GGATCC 1 cut(s) 1078
BanI GGYRCC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 6 cut(s) 49, 71, 89, 1018, 1161, 1363
BccI CCATC 6 cut(s) 292, 478, 836, 860, 1202, 1232
BcgI CGANNNNNNTGC 4 cut(s) 1094, 1128, 1148, 1182
BciT130I CCWGG 5 cut(s) 213, 248, 381, 636, 743
BclI TGATCA 1 cut(s) 948
BcnI CCSGG 2 cut(s) 644, 1356
BcoDI GTCTC 5 cut(s) 12, 41, 439, 702, 1115
BfaI CTAG 3 cut(s) 818, 924, 1209
BisI GCNGC 7 cut(s) 60, 63, 78, 81, 1032, 1175, 1352
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 7 cut(s) 61, 64, 79, 82, 1033, 1176, 1353
Bme1390I CCNGG 7 cut(s) 213, 248, 381, 636, 644, 743, 1356
Bme18I GGWCC 3 cut(s) 208, 407, 731
BmeT110I CYCGRG 2 cut(s) 22, 530
BmgT120I GGNCC 5 cut(s) 208, 383, 407, 731, 1063
BmiI GGNNCC 5 cut(s) 210, 252, 537, 1080, 1273
BmrFI CCNGG 7 cut(s) 213, 248, 381, 636, 644, 743, 1356
BmrI ACTGGG 1 cut(s) 421
BmsI GCATC 5 cut(s) 211, 305, 1043, 1123, 1156
BmuI ACTGGG 1 cut(s) 421
BpmI CTGGAG 2 cut(s) 180, 234
Bpu10I CCTNAGC 2 cut(s) 670, 768
Bpu1102I GCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 157
BpuMI CCSGG 2 cut(s) 644, 1356
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 6 cut(s) 81, 211, 379, 380, 531, 634
BsaXI ACNNNNNCTCC 4 cut(s) 478, 508, 682, 712
Bsc4I CCNNNNNNNGG 6 cut(s) 344, 671, 804, 805, 845, 1281
Bse1I ACTGG 4 cut(s) 34, 349, 427, 1283
Bse21I CCTNAGG 1 cut(s) 894
BseBI CCWGG 5 cut(s) 213, 248, 381, 636, 743
BseDI CCNNGG 6 cut(s) 81, 211, 379, 380, 531, 634
BseGI GGATG 2 cut(s) 284, 1171
BseLI CCNNNNNNNGG 6 cut(s) 344, 671, 804, 805, 845, 1281
BseMII CTCAG 6 cut(s) 489, 560, 684, 693, 885, 1141
BseNI ACTGG 4 cut(s) 34, 349, 427, 1283
BseRI GAGGAG 4 cut(s) 480, 483, 1175, 1257
BseXI GCAGC 6 cut(s) 49, 71, 89, 1018, 1161, 1363
BsgI GTGCAG 1 cut(s) 1160
Bsh1236I CGCG 3 cut(s) 71, 83, 714
Bsh1285I CGRYCG 2 cut(s) 75, 712
BshFI GGCC 2 cut(s) 385, 1065
BshNI GGYRCC 1 cut(s) 250
BsiEI CGRYCG 2 cut(s) 75, 712
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 2 cut(s) 22, 530
BsiSI CCGG 2 cut(s) 644, 1355
BslFI GGGAC 1 cut(s) 1330
BslI CCNNNNNNNGG 6 cut(s) 344, 671, 804, 805, 845, 1281
BsmAI GTCTC 5 cut(s) 12, 41, 439, 702, 1115
BsmBI CGTCTC 1 cut(s) 702
BsmFI GGGAC 1 cut(s) 1330
BsnI GGCC 2 cut(s) 385, 1065
Bso31I GGTCTC 1 cut(s) 439
BsoBI CYCGRG 2 cut(s) 22, 530
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
Bsp1720I GCTNAGC 1 cut(s) 546
BspACI CCGC 8 cut(s) 39, 69, 81, 83, 233, 440, 712, 1025
BspANI GGCC 2 cut(s) 385, 1065
BspCNI CTCAG 6 cut(s) 490, 559, 683, 692, 886, 1140
BspFNI CGCG 3 cut(s) 71, 83, 714
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 5 cut(s) 210, 252, 537, 1080, 1273
BspPI GGATC 6 cut(s) 551, 755, 909, 1073, 1086, 1185
BspT107I GGYRCC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 439
BsrBI CCGCTC 1 cut(s) 233
BsrI ACTGG 4 cut(s) 34, 349, 427, 1283
BssECI CCNNGG 6 cut(s) 81, 211, 379, 380, 531, 634
BssMI GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
Bst2UI CCWGG 5 cut(s) 213, 248, 381, 636, 743
Bst4CI ACNGT 4 cut(s) 92, 109, 859, 1015
Bst6I CTCTTC 2 cut(s) 110, 1068
BstC8I GCNNGC 2 cut(s) 367, 387
BstDEI CTNAG 7 cut(s) 498, 546, 670, 679, 768, 894, 1127
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 284, 1171
BstFNI CGCG 3 cut(s) 71, 83, 714
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 7 cut(s) 75, 559, 763, 917, 951, 1081, 1193
BstMAI GTCTC 5 cut(s) 12, 41, 439, 702, 1115
BstMBI GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
BstMCI CGRYCG 2 cut(s) 75, 712
BstMWI GCNNNNNNNGC 7 cut(s) 68, 77, 101, 230, 661, 1031, 1183
BstNI CCWGG 5 cut(s) 213, 248, 381, 636, 743
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 7 cut(s) 211, 246, 379, 634, 642, 741, 1354
BstUI CGCG 3 cut(s) 71, 83, 714
BstV1I GCAGC 6 cut(s) 49, 71, 89, 1018, 1161, 1363
BstX2I RGATCY 1 cut(s) 1078
BstXI CCANNNNNNTGG 1 cut(s) 1329
BstYI RGATCY 1 cut(s) 1078
Bsu36I CCTNAGG 1 cut(s) 894
BsuRI GGCC 2 cut(s) 385, 1065
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 284, 1171
BtsI GCAGTG 2 cut(s) 1098, 1105
BtsIMutI CAGTG 3 cut(s) 1098, 1105, 1135
Cac8I GCNNGC 2 cut(s) 367, 387
CaiI CAGNNNCTG 3 cut(s) 344, 686, 863
CciI TCATGA 1 cut(s) 418
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 5 cut(s) 208, 383, 407, 731, 1063
Cfr42I CCGCGG 1 cut(s) 84
Csp6I GTAC 2 cut(s) 373, 690
CviAII CATG 2 cut(s) 370, 419
CviQI GTAC 2 cut(s) 373, 690
DdeI CTNAG 7 cut(s) 498, 546, 670, 679, 768, 894, 1127
DpnI GATC 7 cut(s) 74, 558, 762, 916, 950, 1080, 1192
DpnII GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
DraIII CACNNNGTG 1 cut(s) 1130
Eam1104I CTCTTC 2 cut(s) 110, 1068
EarI CTCTTC 2 cut(s) 110, 1068
Eco31I GGTCTC 1 cut(s) 439
Eco47I GGWCC 3 cut(s) 208, 407, 731
Eco57I CTGAAG 1 cut(s) 1002
Eco81I CCTNAGG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 22, 530
EcoRI GAATTC 2 cut(s) 746, 1217
EcoRII CCWGG 5 cut(s) 211, 246, 379, 634, 741
Esp3I CGTCTC 1 cut(s) 702
FaeI CATG 2 cut(s) 373, 422
FalI AAGNNNNNCTT 2 cut(s) 805, 837
FaqI GGGAC 1 cut(s) 1330
FatI CATG 2 cut(s) 369, 418
FbaI TGATCA 1 cut(s) 948
Fnu4HI GCNGC 7 cut(s) 60, 63, 78, 81, 1032, 1175, 1352
FokI GGATG 2 cut(s) 271, 1178
Fsp4HI GCNGC 7 cut(s) 60, 63, 78, 81, 1032, 1175, 1352
FspBI CTAG 3 cut(s) 818, 924, 1209
GlaI GCGC 1 cut(s) 96
GluI GCNGC 7 cut(s) 60, 63, 78, 81, 1032, 1175, 1352
GsuI CTGGAG 2 cut(s) 180, 234
HaeIII GGCC 2 cut(s) 385, 1065
HapII CCGG 2 cut(s) 644, 1355
HhaI GCGC 1 cut(s) 97
Hin1II CATG 2 cut(s) 373, 422
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 5 cut(s) 20, 500, 591, 675, 1247
HpaII CCGG 2 cut(s) 644, 1355
HphI GGTGA 5 cut(s) 230, 327, 429, 587, 770
Hpy166II GTNNAC 1 cut(s) 855
Hpy188I TCNGA 8 cut(s) 114, 313, 588, 752, 865, 982, 1276, 1305
Hpy8I GTNNAC 1 cut(s) 855
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 8 cut(s) 114, 264, 330, 531, 843, 884, 1092, 1300
HpyCH4III ACNGT 4 cut(s) 92, 109, 859, 1015
HpyCH4V TGCA 7 cut(s) 278, 296, 1034, 1136, 1169, 1177, 1351
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 77, 101, 230, 661, 1031, 1183
HpyF3I CTNAG 7 cut(s) 498, 546, 670, 679, 768, 894, 1127
Hsp92II CATG 2 cut(s) 373, 422
HspAI GCGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 948
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
LmnI GCTCC 3 cut(s) 199, 484, 1277
Lsp1109I GCAGC 6 cut(s) 49, 71, 89, 1018, 1161, 1363
LweI GCATC 5 cut(s) 211, 305, 1043, 1123, 1156
MaeI CTAG 3 cut(s) 818, 924, 1209
MaeIII GTNAC 4 cut(s) 32, 325, 832, 1253
MalI GATC 7 cut(s) 74, 558, 762, 916, 950, 1080, 1192
MbiI CCGCTC 1 cut(s) 233
MboI GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
MboII GAAGA 4 cut(s) 127, 296, 1085, 1330
MfeI CAATTG 2 cut(s) 258, 987
MflI RGATCY 1 cut(s) 1078
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 8 cut(s) 258, 393, 746, 942, 987, 1217, 1222, 1327
MlyI GAGTC 2 cut(s) 14, 509
MmeI TCCRAC 2 cut(s) 942, 1299
MseI TTAA 3 cut(s) 12, 905, 974
MslI CAYNNNNRTG 1 cut(s) 148
MspA1I CMGCKG 3 cut(s) 62, 83, 1174
MspI CCGG 2 cut(s) 644, 1355
MspR9I CCNGG 7 cut(s) 213, 248, 381, 636, 644, 743, 1356
MunI CAATTG 2 cut(s) 258, 987
MvaI CCWGG 5 cut(s) 213, 248, 381, 636, 743
MvnI CGCG 3 cut(s) 71, 83, 714
MwoI GCNNNNNNNGC 7 cut(s) 68, 77, 101, 230, 661, 1031, 1183
NciI CCSGG 2 cut(s) 644, 1356
NdeII GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
NlaIII CATG 2 cut(s) 373, 422
NlaIV GGNNCC 5 cut(s) 210, 252, 537, 1080, 1273
NmeAIII GCCGAG 1 cut(s) 1129
NmuCI GTSAC 2 cut(s) 325, 832
NspI RCATGY 1 cut(s) 373
PaeR7I CTCGAG 1 cut(s) 22
PagI TCATGA 1 cut(s) 418
PasI CCCWGGG 1 cut(s) 380
PfeI GAWTC 3 cut(s) 591, 675, 1247
PflMI CCANNNNNTGG 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 7 cut(s) 61, 64, 79, 82, 1033, 1176, 1353
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 2 cut(s) 14, 508
PpsI GAGTC 2 cut(s) 14, 508
PshBI ATTAAT 1 cut(s) 12
Psp6I CCWGG 5 cut(s) 211, 246, 379, 634, 741
PspGI CCWGG 5 cut(s) 211, 246, 379, 634, 741
PspN4I GGNNCC 5 cut(s) 210, 252, 537, 1080, 1273
PspPI GGNCC 5 cut(s) 208, 383, 407, 731, 1063
PstNI CAGNNNCTG 3 cut(s) 344, 686, 863
PsuI RGATCY 1 cut(s) 1078
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 62, 1174
RgaI GCGATCGC 1 cut(s) 75
RsaI GTAC 2 cut(s) 374, 691
RsaNI GTAC 2 cut(s) 373, 690
RseI CAYNNNNRTG 1 cut(s) 148
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 3 cut(s) 12, 905, 974
SatI GCNGC 7 cut(s) 60, 63, 78, 81, 1032, 1175, 1352
Sau3AI GATC 7 cut(s) 72, 556, 760, 914, 948, 1078, 1190
Sau96I GGNCC 5 cut(s) 208, 383, 407, 731, 1063
SchI GAGTC 2 cut(s) 14, 509
ScrFI CCNGG 7 cut(s) 213, 248, 381, 636, 644, 743, 1356
SduI GDGCHC 1 cut(s) 143
SfaAI GCGATCGC 1 cut(s) 75
SfaNI GCATC 5 cut(s) 211, 305, 1043, 1123, 1156
Sfr274I CTCGAG 1 cut(s) 22
Sfr303I CCGCGG 1 cut(s) 84
SgfI GCGATCGC 1 cut(s) 75
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 3 cut(s) 208, 407, 731
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 2 cut(s) 22, 136
SmoI CTYRAG 2 cut(s) 22, 136
Sse9I AATT 8 cut(s) 258, 393, 746, 942, 987, 1217, 1222, 1327
SsiI CCGC 8 cut(s) 39, 69, 81, 83, 233, 440, 712, 1025
SspMI CTAG 3 cut(s) 818, 924, 1209
StyD4I CCNGG 7 cut(s) 211, 246, 379, 634, 642, 741, 1354
TaaI ACNGT 4 cut(s) 92, 109, 859, 1015
TaqI TCGA 3 cut(s) 23, 555, 1259
TaqII GACCGA 2 cut(s) 313, 395
TasI AATT 8 cut(s) 258, 393, 746, 942, 987, 1217, 1222, 1327
TatI WGTACW 1 cut(s) 372
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 3 cut(s) 591, 675, 1247
Tru1I TTAA 3 cut(s) 12, 905, 974
Tru9I TTAA 3 cut(s) 12, 905, 974
TscAI CASTG 3 cut(s) 1098, 1105, 1135
TseFI GTSAC 2 cut(s) 325, 832
TseI GCWGC 6 cut(s) 59, 62, 77, 1031, 1174, 1351
Tsp45I GTSAC 2 cut(s) 325, 832
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 2 cut(s) 221, 606
TspRI CASTG 3 cut(s) 1098, 1105, 1135
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 3 cut(s) 208, 407, 731
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 2 cut(s) 746, 1217
XceI RCATGY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 2 cut(s) 569, 632
XhoI CTCGAG 1 cut(s) 22
XspI CTAG 3 cut(s) 818, 924, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.