MD06G1099300.v1.1

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002846
Physical Location & Seq
Reverse (-)
23568450 .. 23573259
4810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1099300.v1.1.491

Sequence Viewer

Length: 2220 bp
ATGGCGTGGAGAGAGCTTGGCAAAAGGGGGTTATGTGCCAGTATAACTACAGATTTAACTTCAAGACTACAAAGACATGCAATGGGATTTACATCTGTATCTACTGTTGAGGATGTAATAAAATCAGTTTCTGCTGCTGGCAGATGCTTACCAGATCCCCCCTTTAGATTTATGTCAAGAAGGCTTGATTGTTGTGAAGGTTTGACAAAAGATTCCCTGATACGATGCTATCATGCAAGCCCAGAACTGTTTGCCAGAAAAGGAAATGAAGACTCATTTGGTTTAAAAACTCCCAAGAAAGGGAAGTTCAAAAAGAGGGACCATAAGGCCCACCCTCCTGTTGAAGCTCCATATGTGCCTCCTAAACCACAGAGAACTACCAAACCTATGCCAGATAAAATGATCGAGATTTTTGAAGGCATGACAGTTGTTGAACTTGCGAAGCGTACTGGTGAATCAATACCAACTCTGCAAAGTATTCTCACAAATGTCGGGGAAAAGGTTGAAACAGAGTTTGATCCTCTCAGCATCGACGTTTCGGAGCTGATTGCAATGGAAGTTGGGATCAATGTTAGGAGGCTGCACTCCAATAAAGGTACAGAAATTCTACCTCGGCCACCAGTTGTAACTGTCATGGGTCACGTCGACCATGGTAAAACCTCGCTTCTAGATGCTCTACGTCAAACATCTGTGGCTGCCAAGGAAGCTGGAGGAATTACTCAGCATCTAGGTGCTTTCATTGTTGGTATGACATCAGGAGCATCAATTACATTTCTTGACACCCCTGGTCATGCAGCATTTAGTGCGATGCGGGCAAGAGGTGCTGCTGTCACAGATATAGTTGTGCTAGTTGTAGCTGCTGATGATGGGGTGATGCCTCAAACTCTTGAAGCCATGGCTCATGCAAAGGCAGCTAATGTACCGATTGTGGTTGCAATTAATAAATGTGATAAACCGGCTGCTAACCCGGAAAGAATAAAACTCCAGCTTGCTTCCGAGGGTTTGCTGCTGGAGGAGATGGGTGGAGATGTGCAGGTTGTGGAGGTTTCAGCGATAAAGAAGACTGGATTAGATAACTTGGAGGAGGCTTTGCTTCTCCAAGCTGAGATTATGGATCTCAAATCTCGAGTTGATGGGCCTGCTCAAGCTTATGTGGTGGAGGCAAGGCTTGATAAGGGAAAGGGTCCAGCGGTTACAGCAATAGTGAAAGCAGGGACTTTAGAATCCGGGCAGTATGTGGTGGTGGGATCTGAGTGGGGCAGAATAAGGGCTATTAGGGACATGGCGGGGAAGTTGGCAGAGAAGGCAAGACCTGCAATGCCAGTTGAGATTGAAGGGTTGAAGGGACTTCCAAGGGCGGGAGATGATATCATTGTTGTGGAATCTGAGGAGCGAGCTAGAATGCTTAGTGCAGGGAGGAAAAAGAAATTCGAGAGAGATAGACTTAGGAAGATTAAAGCTGAGATGGATGAGAGGAATGAGGAAGAAATATTAAAAGCAAAAAAAGCAGAAGAGTCTGGAGACGAGCCAAAGGAGGAACCCAAGCGGGTGGAATTGCCAGTAATAGTAAAAGCAGATGTGCAGGGCACTGTCCAAGCTGTTACAGACGCATTAATGAATTTAAATAGTCCTCAGGTCTTTGTGAATGTAGTGCATGTTGGTGTTGGGCCTTTATCTCAGTCTGATTTGGACATAGCACAAGCCTGTGGTGCTTGCATTATTGGATTTAATATAAAGACTCCGCCCACTTCCCTCAGTTTGGCTGCAACTCGAGCCAATATAAAGATAATGCTGCATCGTGTTATATATCACCTTCTGGAGGACATTGGCAATTTTATAGTAGACAAAGCCCCTGGAACTTGTGAAACCAAGGTTGCTGGGGAAGCTGAGGTGCTTAGTATTTTTGAGCTCAAAGGAAGGAGCAAGACCAAGGGACCTGATGTGAAGATTGCAGGATGTCGAGTGATTGACGGTTTAATGAGCAAATCGGGAATCTTGAGGCTACTTAGGAGTGGGGAAGTCGTATTTGAAGGATCCTGCGAATCACTCAAGCGGGAGAAACAGGATGTGGACACGGTAAAGAAGGGGAATGACTGTGGACTGGTAATCCAAGATTACGATGATTTCCGAGTTGGAGACATGGTCCAATGCCTGGAGCAAGTTGTAAGGAAGCCCAAATTCATTTCGTCAGCGAGTGGTGCTGCTCGAATAGAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU IF-2

Protein Analysis

740

Amino Acids

80.03

Weight (kDa)

7.84

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 209 - 365 1.2e-33 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 209 - 315 2.4e-09 50S ribosome-binding GTPase
FeoB_N PF02421 209 - 363 7.9e-06 Ferrous iron transport protein B
EF-G_D2 PF22042 380 - 459 8.3e-20 Elongation factor G domain 2
IF-2 PF11987 501 - 609 1.2e-27 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1024, 1321
AccB7I CCANNNNNTGG 1 cut(s) 2152
AccI GTMKAC 2 cut(s) 645, 1842
AciI CCGC 7 cut(s) 811, 1190, 1286, 1358, 1546, 1742, 2053
AclWI GGATC 7 cut(s) 149, 512, 572, 1122, 1255, 2028, 2041
AcoI YGGCCR 1 cut(s) 614
AcsI RAATTY 4 cut(s) 603, 1427, 1618, 2177
AfaI GTAC 3 cut(s) 448, 598, 921
AfiI CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1759, 1819, 2152
AjiI CACGTC 1 cut(s) 643
AjnI CCWGG 3 cut(s) 784, 1852, 2151
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
Alw21I GWGCWC 1 cut(s) 1911
Alw26I GTCTC 2 cut(s) 1515, 2130
AlwI GGATC 7 cut(s) 149, 512, 572, 1122, 1255, 2028, 2041
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 2 cut(s) 1125, 1770
AoxI GGCC 4 cut(s) 327, 614, 1136, 1667
ApoI RAATTY 4 cut(s) 603, 1427, 1618, 2177
AseI ATTAAT 2 cut(s) 939, 1613
AspS9I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1667, 1934, 2143
AsuC2I CCSGG 2 cut(s) 968, 1228
AsuHPI GGTGA 3 cut(s) 464, 883, 1802
AvaI CYCGRG 2 cut(s) 1125, 1770
AvaII GGWCC 4 cut(s) 319, 1184, 1934, 2143
AxyI CCTNAGG 1 cut(s) 1632
BaeGI GKGCMC 1 cut(s) 1589
BaeI ACNNNNGTAYC 2 cut(s) 81, 114
BamHI GGATCC 1 cut(s) 2033
BanII GRGCYC 1 cut(s) 1911
BbsI GAAGAC 2 cut(s) 276, 1067
Bbv12I GWGCWC 1 cut(s) 1911
BbvCI CCTCAGC 1 cut(s) 1887
BccI CCATC 4 cut(s) 860, 1012, 1127, 1459
BcgI CGANNNNNNTGC 1 cut(s) 2196
BciT130I CCWGG 3 cut(s) 786, 1854, 2153
BcnI CCSGG 2 cut(s) 968, 1228
BcoDI GTCTC 2 cut(s) 1515, 2130
BfaI CTAG 4 cut(s) 668, 728, 848, 1398
BfmI CTRYAG 1 cut(s) 48
BfuAI ACCTGC 2 cut(s) 1024, 1321
Bme1390I CCNGG 5 cut(s) 786, 968, 1228, 1854, 2153
Bme18I GGWCC 4 cut(s) 319, 1184, 1934, 2143
BmeT110I CYCGRG 2 cut(s) 1125, 1770
BmgBI CACGTC 1 cut(s) 643
BmgT120I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1667, 1934, 2143
BmiI GGNNCC 5 cut(s) 320, 1185, 1539, 1935, 2035
BmrFI CCNGG 5 cut(s) 786, 968, 1228, 1854, 2153
BmsI GCATC 9 cut(s) 134, 215, 537, 661, 733, 770, 798, 864, 1804
BpiI GAAGAC 2 cut(s) 276, 1067
BpmI CTGGAG 6 cut(s) 729, 968, 1031, 1539, 1838, 2174
Bpu10I CCTNAGC 1 cut(s) 1887
BpuEI CTTGAG 3 cut(s) 1128, 2017, 2033
BpuMI CCSGG 2 cut(s) 968, 1228
BsaBI GATNNNNATC 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 2127, 2157
Bsc4I CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1759, 1819, 2152
Bse118I RCCGGY 1 cut(s) 955
Bse1I ACTGG 7 cut(s) 39, 454, 620, 1069, 1322, 1559, 2106
Bse21I CCTNAGG 1 cut(s) 1632
Bse3DI GCAATG 3 cut(s) 87, 558, 1323
Bse8I GATNNNNATC 1 cut(s) 91
BseBI CCWGG 3 cut(s) 786, 1854, 2153
BseGI GGATG 4 cut(s) 118, 1474, 1961, 2071
BseJI GATNNNNATC 1 cut(s) 91
BseLI CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1759, 1819, 2152
BseMI GCAATG 3 cut(s) 87, 558, 1323
BseNI ACTGG 7 cut(s) 39, 454, 620, 1069, 1322, 1559, 2106
BseRI GAGGAG 3 cut(s) 1028, 1097, 1403
BseSI GKGCMC 1 cut(s) 1589
BseYI CCCAGC 1 cut(s) 1877
BsgI GTGCAG 4 cut(s) 566, 1052, 1431, 1601
BshFI GGCC 4 cut(s) 329, 616, 1138, 1669
BsiHKAI GWGCWC 1 cut(s) 1911
BsiHKCI CYCGRG 2 cut(s) 1125, 1770
BsiSI CCGG 3 cut(s) 956, 968, 1227
BslFI GGGAC 5 cut(s) 332, 1228, 1292, 1359, 1947
BslI CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1759, 1819, 2152
BsmAI GTCTC 2 cut(s) 1515, 2130
BsmBI CGTCTC 1 cut(s) 1515
BsmFI GGGAC 5 cut(s) 332, 1228, 1292, 1359, 1947
BsmI GAATGC 1 cut(s) 1407
BsnI GGCC 4 cut(s) 329, 616, 1138, 1669
BsoBI CYCGRG 2 cut(s) 1125, 1770
Bsp1286I GDGCHC 2 cut(s) 1589, 1911
Bsp143I GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
Bsp19I CCATGG 2 cut(s) 649, 894
BspACI CCGC 7 cut(s) 811, 1190, 1286, 1358, 1546, 1742, 2053
BspANI GGCC 4 cut(s) 329, 616, 1138, 1669
BspLI GGNNCC 5 cut(s) 320, 1185, 1539, 1935, 2035
BspMI ACCTGC 2 cut(s) 1024, 1321
BspPI GGATC 7 cut(s) 149, 512, 572, 1122, 1255, 2028, 2041
BsrDI GCAATG 3 cut(s) 87, 558, 1323
BsrFI RCCGGY 1 cut(s) 955
BsrI ACTGG 7 cut(s) 39, 454, 620, 1069, 1322, 1559, 2106
BssAI RCCGGY 1 cut(s) 955
BssMI GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
BssT1I CCWWGG 6 cut(s) 649, 699, 894, 1352, 1869, 1929
Bst2UI CCWGG 3 cut(s) 786, 1854, 2153
Bst4CI ACNGT 8 cut(s) 106, 249, 427, 631, 1591, 1973, 2077, 2096
Bst6I CTCTTC 1 cut(s) 1506
BstAPI GCANNNNNTGC 3 cut(s) 803, 821, 1313
BstC8I GCNNGC 7 cut(s) 139, 238, 813, 990, 1140, 1395, 1714
BstDSI CCRYGG 2 cut(s) 649, 894
BstENI CCTNNNNNAGG 1 cut(s) 1817
BstF5I GGATG 4 cut(s) 118, 1474, 1961, 2071
BstKTI GATC 7 cut(s) 157, 405, 520, 567, 1117, 1250, 2036
BstMAI GTCTC 2 cut(s) 1515, 2130
BstMBI GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
BstNI CCWGG 3 cut(s) 786, 1854, 2153
BstNSI RCATGY 2 cut(s) 80, 1658
BstSCI CCNGG 5 cut(s) 784, 966, 1226, 1852, 2151
BstSFI CTRYAG 1 cut(s) 48
BstSLI GKGCMC 1 cut(s) 1589
BstV2I GAAGAC 2 cut(s) 276, 1067
BstX2I RGATCY 4 cut(s) 154, 1114, 1247, 2033
BstXI CCANNNNNNTGG 1 cut(s) 1549
BstYI RGATCY 4 cut(s) 154, 1114, 1247, 2033
Bsu36I CCTNAGG 1 cut(s) 1632
BsuRI GGCC 4 cut(s) 329, 616, 1138, 1669
BtgI CCRYGG 2 cut(s) 649, 894
BtgZI GCGATG 1 cut(s) 821
BtrI CACGTC 1 cut(s) 643
BtsCI GGATG 4 cut(s) 118, 1474, 1961, 2071
BtsIMutI CAGTG 1 cut(s) 1587
BveI ACCTGC 2 cut(s) 1024, 1321
Cac8I GCNNGC 7 cut(s) 139, 238, 813, 990, 1140, 1395, 1714
CaiI CAGNNNCTG 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 955
Cfr13I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1667, 1934, 2143
CseI GACGC 1 cut(s) 1616
Csp6I GTAC 3 cut(s) 447, 597, 920
CviQI GTAC 3 cut(s) 447, 597, 920
DpnI GATC 7 cut(s) 156, 404, 519, 566, 1116, 1249, 2035
DpnII GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
DraI TTTAAA 2 cut(s) 285, 1623
EaeI YGGCCR 1 cut(s) 614
Eam1104I CTCTTC 1 cut(s) 1506
EarI CTCTTC 1 cut(s) 1506
EciI GGCGGA 1 cut(s) 1731
Ecl136II GAGCTC 1 cut(s) 1909
Eco130I CCWWGG 6 cut(s) 649, 699, 894, 1352, 1869, 1929
Eco24I GRGCYC 1 cut(s) 1911
Eco32I GATATC 1 cut(s) 1369
Eco47I GGWCC 4 cut(s) 319, 1184, 1934, 2143
Eco53kI GAGCTC 1 cut(s) 1909
Eco81I CCTNAGG 1 cut(s) 1632
Eco88I CYCGRG 2 cut(s) 1125, 1770
EcoICRI GAGCTC 1 cut(s) 1909
EcoNI CCTNNNNNAGG 1 cut(s) 1817
EcoO109I RGGNCCY 1 cut(s) 1934
EcoRII CCWGG 3 cut(s) 784, 1852, 2151
EcoRV GATATC 1 cut(s) 1369
EcoT14I CCWWGG 6 cut(s) 649, 699, 894, 1352, 1869, 1929
EcoT38I GRGCYC 1 cut(s) 1911
ErhI CCWWGG 6 cut(s) 649, 699, 894, 1352, 1869, 1929
Esp3I CGTCTC 1 cut(s) 1515
FaqI GGGAC 5 cut(s) 332, 1228, 1292, 1359, 1947
FauI CCCGC 5 cut(s) 804, 1279, 1351, 1539, 2046
FauNDI CATATG 1 cut(s) 352
FblI GTMKAC 2 cut(s) 645, 1842
FokI GGATG 4 cut(s) 125, 1481, 1968, 2078
FriOI GRGCYC 1 cut(s) 1911
FspBI CTAG 4 cut(s) 668, 728, 848, 1398
GsaI CCCAGC 1 cut(s) 1881
GsuI CTGGAG 6 cut(s) 729, 968, 1031, 1539, 1838, 2174
HaeIII GGCC 4 cut(s) 329, 616, 1138, 1669
HapII CCGG 3 cut(s) 956, 968, 1227
HgaI GACGC 1 cut(s) 1616
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 1 cut(s) 1146
HinfI GANTC 9 cut(s) 212, 272, 455, 1223, 1382, 1514, 1738, 1992, 2042
HpaII CCGG 3 cut(s) 956, 968, 1227
HphI GGTGA 3 cut(s) 464, 883, 1802
Hpy166II GTNNAC 4 cut(s) 646, 1843, 2071, 2099
Hpy188I TCNGA 6 cut(s) 541, 997, 1252, 1387, 1684, 2129
Hpy8I GTNNAC 4 cut(s) 646, 1843, 2071, 2099
Hpy99I CGWCG 2 cut(s) 536, 647
HpyCH4III ACNGT 8 cut(s) 106, 249, 427, 631, 1591, 1973, 2077, 2096
HpyCH4IV ACGT 3 cut(s) 534, 642, 679
HpySE526I ACGT 3 cut(s) 534, 642, 679
Kzo9I GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
LmnI GCTCC 6 cut(s) 352, 541, 758, 1390, 1920, 2155
LweI GCATC 9 cut(s) 134, 215, 537, 661, 733, 770, 798, 864, 1804
MaeI CTAG 4 cut(s) 668, 728, 848, 1398
MaeII ACGT 3 cut(s) 534, 642, 679
MaeIII GTNAC 5 cut(s) 625, 638, 829, 1192, 1600
MalI GATC 7 cut(s) 156, 404, 519, 566, 1116, 1249, 2035
MboI GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
MboII GAAGA 6 cut(s) 281, 1072, 1462, 1496, 1523, 1957
MflI RGATCY 4 cut(s) 154, 1114, 1247, 2033
MhlI GDGCHC 2 cut(s) 1589, 1911
MluCI AATT 9 cut(s) 603, 714, 765, 936, 1427, 1553, 1618, 1831, 2177
MlyI GAGTC 3 cut(s) 266, 1523, 1732
MmeI TCCRAC 1 cut(s) 2113
MseI TTAA 9 cut(s) 56, 284, 939, 1455, 1493, 1613, 1622, 1728, 1976
MslI CAYNNNNRTG 3 cut(s) 729, 1376, 1659
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 3 cut(s) 956, 968, 1227
MspR9I CCNGG 5 cut(s) 786, 968, 1228, 1854, 2153
Mva1269I GAATGC 1 cut(s) 1407
MvaI CCWGG 3 cut(s) 786, 1854, 2153
NciI CCSGG 2 cut(s) 968, 1228
NcoI CCATGG 2 cut(s) 649, 894
NdeI CATATG 1 cut(s) 352
NdeII GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
NlaIV GGNNCC 5 cut(s) 320, 1185, 1539, 1935, 2035
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 2 cut(s) 638, 829
NspI RCATGY 2 cut(s) 80, 1658
PaeR7I CTCGAG 2 cut(s) 1125, 1770
PctI GAATGC 1 cut(s) 1407
PfeI GAWTC 6 cut(s) 212, 455, 1223, 1382, 1992, 2042
PflFI GACNNNGTC 1 cut(s) 2141
PflMI CCANNNNNTGG 1 cut(s) 2152
PleI GAGTC 3 cut(s) 266, 1522, 1732
PpsI GAGTC 3 cut(s) 266, 1522, 1732
PpuMI RGGWCCY 1 cut(s) 1934
PshBI ATTAAT 2 cut(s) 939, 1613
Psp124BI GAGCTC 1 cut(s) 1911
Psp5II RGGWCCY 1 cut(s) 1934
Psp6I CCWGG 3 cut(s) 784, 1852, 2151
PspFI CCCAGC 1 cut(s) 1877
PspGI CCWGG 3 cut(s) 784, 1852, 2151
PspN4I GGNNCC 5 cut(s) 320, 1185, 1539, 1935, 2035
PspPI GGNCC 7 cut(s) 319, 328, 1136, 1184, 1667, 1934, 2143
PspPPI RGGWCCY 1 cut(s) 1934
PspXI VCTCGAGB 1 cut(s) 1770
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 4 cut(s) 154, 1114, 1247, 2033
PsyI GACNNNGTC 1 cut(s) 2141
RsaI GTAC 3 cut(s) 448, 598, 921
RsaNI GTAC 3 cut(s) 447, 597, 920
RseI CAYNNNNRTG 3 cut(s) 729, 1376, 1659
SacI GAGCTC 1 cut(s) 1911
SalI GTCGAC 1 cut(s) 644
SaqAI TTAA 9 cut(s) 56, 284, 939, 1455, 1493, 1613, 1622, 1728, 1976
Sau3AI GATC 7 cut(s) 154, 402, 517, 564, 1114, 1247, 2033
Sau96I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1667, 1934, 2143
SchI GAGTC 3 cut(s) 266, 1523, 1732
ScrFI CCNGG 5 cut(s) 786, 968, 1228, 1854, 2153
SduI GDGCHC 2 cut(s) 1589, 1911
SfaNI GCATC 9 cut(s) 134, 215, 537, 661, 733, 770, 798, 864, 1804
SfcI CTRYAG 1 cut(s) 48
Sfr274I CTCGAG 2 cut(s) 1125, 1770
SinI GGWCC 4 cut(s) 319, 1184, 1934, 2143
SlaI CTCGAG 2 cut(s) 1125, 1770
SmiI ATTTAAAT 1 cut(s) 1623
SmiMI CAYNNNNRTG 3 cut(s) 729, 1376, 1659
SmlI CTYRAG 5 cut(s) 1125, 1143, 1770, 1996, 2048
SmoI CTYRAG 5 cut(s) 1125, 1143, 1770, 1996, 2048
Sse9I AATT 9 cut(s) 603, 714, 765, 936, 1427, 1553, 1618, 1831, 2177
SsiI CCGC 7 cut(s) 811, 1190, 1286, 1358, 1546, 1742, 2053
SspI AATATT 1 cut(s) 1491
SspMI CTAG 4 cut(s) 668, 728, 848, 1398
SstI GAGCTC 1 cut(s) 1911
StyD4I CCNGG 5 cut(s) 784, 966, 1226, 1852, 2151
StyI CCWWGG 6 cut(s) 649, 699, 894, 1352, 1869, 1929
SwaI ATTTAAAT 1 cut(s) 1623
TaaI ACNGT 8 cut(s) 106, 249, 427, 631, 1591, 1973, 2077, 2096
TaiI ACGT 3 cut(s) 537, 645, 682
TaqI TCGA 8 cut(s) 405, 531, 645, 1126, 1431, 1771, 1960, 2206
TasI AATT 9 cut(s) 603, 714, 765, 936, 1427, 1553, 1618, 1831, 2177
TfiI GAWTC 6 cut(s) 212, 455, 1223, 1382, 1992, 2042
Tru1I TTAA 9 cut(s) 56, 284, 939, 1455, 1493, 1613, 1622, 1728, 1976
Tru9I TTAA 9 cut(s) 56, 284, 939, 1455, 1493, 1613, 1622, 1728, 1976
TscAI CASTG 1 cut(s) 1594
TseFI GTSAC 2 cut(s) 638, 829
Tsp45I GTSAC 2 cut(s) 638, 829
TspDTI ATGAA 4 cut(s) 282, 727, 1631, 2170
TspRI CASTG 1 cut(s) 1594
Tth111I GACNNNGTC 1 cut(s) 2141
Van91I CCANNNNNTGG 1 cut(s) 2152
VpaK11BI GGWCC 4 cut(s) 319, 1184, 1934, 2143
VspI ATTAAT 2 cut(s) 939, 1613
XagI CCTNNNNNAGG 1 cut(s) 1817
XapI RAATTY 4 cut(s) 603, 1427, 1618, 2177
XbaI TCTAGA 1 cut(s) 667
XceI RCATGY 2 cut(s) 80, 1658
XhoI CTCGAG 2 cut(s) 1125, 1770
XmiI GTMKAC 2 cut(s) 645, 1842
XspI CTAG 4 cut(s) 668, 728, 848, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.