Rh1CG096500

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002846
Physical Location & Seq
Forward (+)
20685519 .. 20688696
3178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG096500.1

Sequence Viewer

Length: 2046 bp
ATGCCATTATCATATCCTTACTTTTGTGCTGCAGAGCCCTCATTTAAAGTTAAGTCACGAAAGCTTGTGTGTGGTGAAATATTGATAAAAGACATCCAAATACGATGCTATCATGGGAGAAATGGGAATGACCAGTCATTTGGTTTAAAACCTCCTAAGAGAGAGAAGTTTGTAAAGAGGGACAATAAGGCACAGCCTCCTGTTGATGCTCCATATGTGCCTCCTAAACCACAAAGAACTACCAAGGCCATGCCAGATAAGACAATTGAGATTTTTGAAGGTATGACAATTGATGAACTTGCCAAGCGCACTGGACAGTCAATATCTACTCTGCAAACCATTCTTACAAATGTTGGGGAAAAGGTTGGCTCGGAGTTTGACACTCTCAGCGTCGACATTGCAGAGCTGGTTGCAATGGAAGTTGGGATCAATGTGAGGAGGCTGCACTCCAATGAAGGTACAGAAATTCTACCACGACCTCCAGTTGTAACTGTCATGGGCCATGTTGACCATGGGAAAACTTCACTTTTGGATGCTCTACGTCAGACATCTGTGGCTGCCAAAGAAGCTGGAGGGATTACTCAGCATGTTGGTGCTTTTGTTGTTGGCATGACATCTGGAGCGTCAATTACATTTCTTGACACCCCTGGTCATGCAGCATTTAGTGCGATGCGAGCTAGAGGGGCTGCCGTTACAGATATAGTTGTGCTTGTTGTAGCTGCTGATGATGGGGTGATGCCTCAGACTCTTGAAGCCATGGCCCATGCAAAGGCAGCTAAAGTACCAATTGTTGTTGCAATTAATAAATGTGATAAACCAGCTGCTAATGCAGAAAAAGTAAGACTCCAGCTTGCGTCGGAGGGCTTGCTGCTAGAGGATATGGGTGGGGATGTTCAGGTTGTCGAGGTTTCAGCTATGAAGAAGACTGGATTAGATAACTTGGAGGAGGCGTTGCTTCTCCAGGCTGAGATGATGGATCTGAAAGCTCGTATTGATGGACCGGCCCAAGCTTATGTGGTGGAAGCAAGACTTGATAGGGGAAAGGGTCCATTGGTTACTGCAATAGTGAAAGCAGGAACTTTAATTTGTGGGAAATATGTCGTCATGGGCTCCGAGTGGGGTAGAATAAGGGCTATTCGGGACATGGCAGGGAAGTCGACAGAGCGGGCAACACCTGCGATGCCGGTTGAGATTGAAGGCCTGAAGGGACTTCCAAGGGCTGGAGATGATATCATTGTTGTGGAATCTGAGGAGCGAGCTAGAATGCTTAGTGCAGGGAGGAAGAGGAAGTTCGAGAAAGATAGACTCATGAAAATTCTGGATGGAAGGGTAGAAGATTCAGAAACAGAACCATCAGATGAGGCGCCTAAGCGGGTTGAATTGCCAATAATAGTAAAAGGGGATGTGCAGGGTACTGTCCAAGCTGTTACAGATGCATTAGGGAGTTTAAATAGTCCTCAGGTTTTTGTGAATGTGGTACATGTTGGTGTTGGGTCACTGTCTCAGTCTGATGTGGACATGGCACAAGCCTGTGGTGCATGTATTATTGGATTTAACATCAAGGCTCCACCCAGTTCCATCAGTTTGGCTGCGTCTCGAGCCAATATCAAGATAATGCAACACCGTGTGATCTATCATCTTCTGGAGGACATTGGGAACTTTATAGTTAAGAAGGCCCCTGGGACTACCGAGACCAAGGTTGCTGGGGAAGCCGAGGTTCTTAGTATTTTTGAGCTCAAAGGAAGGAGCAAGTCTAAGGGACCTGATGTGAAGATTGCTGGATGCCGGGTGGTTGATGGTTTTGTCACTAAATCGGCAACCCTAAGGCTTCTTAGGAGTGGAGAAGTTATATTTGAGGGATCATGTGAATCGCTTAAGCGGGAGAAACAGGATGTGGACACGGTAAAGAAGGGGAATGAGTGTGGACTGGTGATCCAAAATTGCTATGATTTCCAGGTTGGTGACATGGTCCAGTGCTTGCAGCAAGTTATAAGGAAGCCCAAATTCATTTCATCGGAGAGTGGTGCTGTTCGAATAGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU IF-2

Protein Analysis

681

Amino Acids

73.38

Weight (kDa)

8.09

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 163 - 319 3.4e-33 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 163 - 269 1.4e-09 50S ribosome-binding GTPase
FeoB_N PF02421 163 - 317 4.3e-06 Ferrous iron transport protein B
EF-G_D2 PF22042 334 - 413 7.9e-19 Elongation factor G domain 2
IF-2 PF11987 457 - 551 2.7e-28 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1991
AarI CACCTGC 1 cut(s) 1183
Acc36I ACCTGC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 1363
AccB7I CCANNNNNTGG 1 cut(s) 1220
AccBSI CCGCTC 1 cut(s) 1165
AccI GTMKAC 2 cut(s) 393, 1157
AciI CCGC 3 cut(s) 1165, 1372, 1879
AclWI GGATC 4 cut(s) 434, 984, 1867, 1927
AcsI RAATTY 3 cut(s) 465, 1314, 2003
AcuI CTGAAG 1 cut(s) 1223
AcyI GRCGYC 1 cut(s) 1364
AdeI CACNNNGTG 1 cut(s) 1627
AfaI GTAC 4 cut(s) 460, 783, 1414, 1479
AfiI CCNNNNNNNGG 2 cut(s) 769, 1220
AflII CTTAAG 1 cut(s) 1874
AflIII ACRYGT 1 cut(s) 1480
AgsI TTSAA 4 cut(s) 278, 752, 1196, 1379
AjnI CCWGG 4 cut(s) 646, 960, 1678, 1953
Alw21I GWGCWC 1 cut(s) 1737
Alw26I GTCTC 3 cut(s) 1506, 1599, 1685
AlwI GGATC 4 cut(s) 434, 984, 1867, 1927
Ama87I CYCGRG 1 cut(s) 1596
AoxI GGCC 6 cut(s) 246, 499, 759, 1002, 1198, 1674
ApoI RAATTY 3 cut(s) 465, 1314, 2003
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AseI ATTAAT 1 cut(s) 801
AspLEI GCGC 2 cut(s) 309, 1366
AspS9I GGNCC 8 cut(s) 499, 760, 998, 1003, 1046, 1675, 1760, 1969
AsuC2I CCSGG 1 cut(s) 1787
AsuHPI GGTGA 4 cut(s) 86, 745, 1942, 1973
AsuII TTCGAA 1 cut(s) 2032
AvaI CYCGRG 1 cut(s) 1596
AvaII GGWCC 4 cut(s) 998, 1046, 1760, 1969
AxyI CCTNAGG 2 cut(s) 1458, 1823
BanI GGYRCC 1 cut(s) 1363
BanII GRGCYC 3 cut(s) 39, 1112, 1737
BbsI GAAGAC 1 cut(s) 929
Bbv12I GWGCWC 1 cut(s) 1737
BccI CCATC 7 cut(s) 722, 967, 989, 1316, 1360, 1586, 1790
BceAI ACGGC 1 cut(s) 674
BcgI CGANNNNNNTGC 2 cut(s) 1137, 1171
BciT130I CCWGG 4 cut(s) 648, 962, 1680, 1955
BcnI CCSGG 1 cut(s) 1787
BcoDI GTCTC 3 cut(s) 1506, 1599, 1685
BfaI CTAG 3 cut(s) 678, 872, 1260
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 1367
BfrI CTTAAG 1 cut(s) 1874
BfuAI ACCTGC 1 cut(s) 1183
Bme1390I CCNGG 5 cut(s) 648, 962, 1680, 1787, 1955
Bme18I GGWCC 4 cut(s) 998, 1046, 1760, 1969
BmeT110I CYCGRG 1 cut(s) 1596
BmgT120I GGNCC 8 cut(s) 499, 760, 998, 1003, 1046, 1675, 1760, 1969
BmiI GGNNCC 6 cut(s) 1047, 1111, 1365, 1566, 1677, 1761
BmrFI CCNGG 5 cut(s) 648, 962, 1680, 1787, 1955
BmrI ACTGGG 1 cut(s) 1566
BmsI GCATC 8 cut(s) 95, 196, 523, 660, 726, 1170, 1423, 1772
BmuI ACTGGG 1 cut(s) 1566
BpiI GAAGAC 1 cut(s) 929
BpmI CTGGAG 7 cut(s) 465, 591, 639, 830, 944, 1242, 1664
Bpu10I CCTNAGC 1 cut(s) 1368
Bpu14I TTCGAA 1 cut(s) 2032
BpuMI CCSGG 1 cut(s) 1787
BsaHI GRCGYC 1 cut(s) 1364
BsaI GGTCTC 1 cut(s) 1685
BsaJI CCNNGG 9 cut(s) 243, 511, 646, 756, 1214, 1678, 1679, 1695, 1713
BsaXI ACNNNNNCTCC 2 cut(s) 935, 965
Bsc4I CCNNNNNNNGG 2 cut(s) 769, 1220
Bse118I RCCGGY 2 cut(s) 1000, 1183
Bse1I ACTGG 7 cut(s) 133, 316, 482, 931, 1572, 1932, 1972
Bse21I CCTNAGG 2 cut(s) 1458, 1823
Bse3DI GCAATG 2 cut(s) 396, 420
BseBI CCWGG 4 cut(s) 648, 962, 1680, 1955
BseDI CCNNGG 9 cut(s) 243, 511, 646, 756, 1214, 1678, 1679, 1695, 1713
BseGI GGATG 7 cut(s) 93, 538, 895, 1327, 1408, 1787, 1897
BseLI CCNNNNNNNGG 2 cut(s) 769, 1220
BseMI GCAATG 2 cut(s) 396, 420
BseMII CTCAG 7 cut(s) 400, 596, 755, 957, 1239, 1472, 1517
BseNI ACTGG 7 cut(s) 133, 316, 482, 931, 1572, 1932, 1972
BseRI GAGGAG 3 cut(s) 451, 959, 1265
BseYI CCCAGC 1 cut(s) 1703
BsgI GTGCAG 3 cut(s) 428, 1293, 1427
BshFI GGCC 6 cut(s) 248, 501, 761, 1004, 1200, 1676
BshNI GGYRCC 1 cut(s) 1363
BsiHKAI GWGCWC 1 cut(s) 1737
BsiHKCI CYCGRG 1 cut(s) 1596
BsiSI CCGG 3 cut(s) 1001, 1184, 1786
BslFI GGGAC 5 cut(s) 194, 1154, 1221, 1696, 1773
BslI CCNNNNNNNGG 2 cut(s) 769, 1220
BsmAI GTCTC 3 cut(s) 1506, 1599, 1685
BsmBI CGTCTC 1 cut(s) 1599
BsmFI GGGAC 5 cut(s) 194, 1154, 1221, 1696, 1773
BsmI GAATGC 1 cut(s) 1269
BsnI GGCC 6 cut(s) 248, 501, 761, 1004, 1200, 1676
Bso31I GGTCTC 1 cut(s) 1685
BsoBI CYCGRG 1 cut(s) 1596
Bsp119I TTCGAA 1 cut(s) 2032
Bsp1286I GDGCHC 3 cut(s) 39, 1112, 1737
Bsp143I GATC 5 cut(s) 426, 976, 1629, 1859, 1932
Bsp19I CCATGG 2 cut(s) 511, 756
BspACI CCGC 3 cut(s) 1165, 1372, 1879
BspANI GGCC 6 cut(s) 248, 501, 761, 1004, 1200, 1676
BspCNI CTCAG 7 cut(s) 399, 595, 754, 958, 1240, 1471, 1516
BspHI TCATGA 1 cut(s) 1308
BspLI GGNNCC 6 cut(s) 1047, 1111, 1365, 1566, 1677, 1761
BspMAI CTGCAG 1 cut(s) 34
BspMI ACCTGC 1 cut(s) 1183
BspPI GGATC 4 cut(s) 434, 984, 1867, 1927
BspT104I TTCGAA 1 cut(s) 2032
BspT107I GGYRCC 1 cut(s) 1363
BspTI CTTAAG 1 cut(s) 1874
BspTNI GGTCTC 1 cut(s) 1685
BsrBI CCGCTC 1 cut(s) 1165
BsrDI GCAATG 2 cut(s) 396, 420
BsrFI RCCGGY 2 cut(s) 1000, 1183
BsrI ACTGG 7 cut(s) 133, 316, 482, 931, 1572, 1932, 1972
BssAI RCCGGY 2 cut(s) 1000, 1183
BssECI CCNNGG 9 cut(s) 243, 511, 646, 756, 1214, 1678, 1679, 1695, 1713
BssMI GATC 5 cut(s) 426, 976, 1629, 1859, 1932
BssNI GRCGYC 1 cut(s) 1364
BssT1I CCWWGG 5 cut(s) 243, 511, 756, 1214, 1695
Bst2UI CCWGG 4 cut(s) 648, 962, 1680, 1955
Bst4CI ACNGT 6 cut(s) 318, 493, 1417, 1500, 1625, 1903
Bst6I CTCTTC 1 cut(s) 1277
BstACI GRCGYC 1 cut(s) 1364
BstAFI CTTAAG 1 cut(s) 1874
BstAPI GCANNNNNTGC 2 cut(s) 665, 1175
BstBI TTCGAA 1 cut(s) 2032
BstC8I GCNNGC 6 cut(s) 675, 852, 866, 1167, 1257, 1979
BstDSI CCRYGG 2 cut(s) 511, 756
BstF5I GGATG 7 cut(s) 93, 538, 895, 1327, 1408, 1787, 1897
BstH2I RGCGCY 1 cut(s) 1367
BstHHI GCGC 2 cut(s) 309, 1366
BstKTI GATC 5 cut(s) 429, 979, 1632, 1862, 1935
BstMAI GTCTC 3 cut(s) 1506, 1599, 1685
BstMBI GATC 5 cut(s) 426, 976, 1629, 1859, 1932
BstNI CCWGG 4 cut(s) 648, 962, 1680, 1955
BstNSI RCATGY 3 cut(s) 590, 1484, 1542
BstSCI CCNGG 5 cut(s) 646, 960, 1678, 1785, 1953
BstSFI CTRYAG 1 cut(s) 30
BstV2I GAAGAC 1 cut(s) 929
BstX2I RGATCY 1 cut(s) 976
BstXI CCANNNNNNTGG 2 cut(s) 140, 1585
BstYI RGATCY 1 cut(s) 976
Bsu36I CCTNAGG 2 cut(s) 1458, 1823
BsuRI GGCC 6 cut(s) 248, 501, 761, 1004, 1200, 1676
BtgI CCRYGG 2 cut(s) 511, 756
BtgZI GCGATG 2 cut(s) 683, 1193
BtsCI GGATG 7 cut(s) 93, 538, 895, 1327, 1408, 1787, 1897
BtsIMutI CAGTG 3 cut(s) 309, 1496, 1979
BveI ACCTGC 1 cut(s) 1183
Cac8I GCNNGC 6 cut(s) 675, 852, 866, 1167, 1257, 1979
CciI TCATGA 1 cut(s) 1308
CfoI GCGC 2 cut(s) 309, 1366
Cfr10I RCCGGY 2 cut(s) 1000, 1183
Cfr13I GGNCC 8 cut(s) 499, 760, 998, 1003, 1046, 1675, 1760, 1969
CseI GACGC 4 cut(s) 379, 612, 843, 1581
Csp6I GTAC 4 cut(s) 459, 782, 1413, 1478
CviQI GTAC 4 cut(s) 459, 782, 1413, 1478
DinI GGCGCC 1 cut(s) 1365
DpnI GATC 5 cut(s) 428, 978, 1631, 1861, 1934
DpnII GATC 5 cut(s) 426, 976, 1629, 1859, 1932
DraI TTTAAA 3 cut(s) 46, 147, 1449
DraIII CACNNNGTG 1 cut(s) 1627
Eam1104I CTCTTC 1 cut(s) 1277
EarI CTCTTC 1 cut(s) 1277
Ecl136II GAGCTC 1 cut(s) 1735
Eco130I CCWWGG 5 cut(s) 243, 511, 756, 1214, 1695
Eco147I AGGCCT 1 cut(s) 1200
Eco24I GRGCYC 3 cut(s) 39, 1112, 1737
Eco31I GGTCTC 1 cut(s) 1685
Eco32I GATATC 1 cut(s) 1231
Eco47I GGWCC 4 cut(s) 998, 1046, 1760, 1969
Eco53kI GAGCTC 1 cut(s) 1735
Eco57I CTGAAG 1 cut(s) 1223
Eco81I CCTNAGG 2 cut(s) 1458, 1823
Eco88I CYCGRG 1 cut(s) 1596
EcoICRI GAGCTC 1 cut(s) 1735
EcoO109I RGGNCCY 2 cut(s) 1675, 1760
EcoRII CCWGG 4 cut(s) 646, 960, 1678, 1953
EcoRV GATATC 1 cut(s) 1231
EcoT14I CCWWGG 5 cut(s) 243, 511, 756, 1214, 1695
EcoT22I ATGCAT 1 cut(s) 1438
EcoT38I GRGCYC 3 cut(s) 39, 1112, 1737
EgeI GGCGCC 1 cut(s) 1365
EheI GGCGCC 1 cut(s) 1365
ErhI CCWWGG 5 cut(s) 243, 511, 756, 1214, 1695
Esp3I CGTCTC 1 cut(s) 1599
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 5 cut(s) 194, 1154, 1221, 1696, 1773
FauI CCCGC 3 cut(s) 1158, 1365, 1872
FauNDI CATATG 1 cut(s) 214
FblI GTMKAC 2 cut(s) 393, 1157
FokI GGATG 7 cut(s) 80, 545, 902, 1334, 1415, 1794, 1904
FriOI GRGCYC 3 cut(s) 39, 1112, 1737
FspBI CTAG 3 cut(s) 678, 872, 1260
GlaI GCGC 2 cut(s) 308, 1365
GsaI CCCAGC 1 cut(s) 1707
GsuI CTGGAG 7 cut(s) 465, 591, 639, 830, 944, 1242, 1664
HaeII RGCGCY 1 cut(s) 1367
HaeIII GGCC 6 cut(s) 248, 501, 761, 1004, 1200, 1676
HapII CCGG 3 cut(s) 1001, 1184, 1786
HgaI GACGC 4 cut(s) 379, 612, 843, 1581
HhaI GCGC 2 cut(s) 309, 1366
Hin1I GRCGYC 1 cut(s) 1364
Hin6I GCGC 2 cut(s) 307, 1364
HinP1I GCGC 2 cut(s) 307, 1364
HincII GTYRAC 3 cut(s) 394, 508, 1158
HindII GTYRAC 3 cut(s) 394, 508, 1158
HindIII AAGCTT 2 cut(s) 62, 1008
HinfI GANTC 6 cut(s) 745, 843, 1244, 1305, 1337, 1868
HpaII CCGG 3 cut(s) 1001, 1184, 1786
HphI GGTGA 4 cut(s) 86, 745, 1942, 1973
Hpy166II GTNNAC 6 cut(s) 394, 508, 1158, 1516, 1897, 1925
Hpy8I GTNNAC 6 cut(s) 394, 508, 1158, 1516, 1897, 1925
Hpy99I CGWCG 2 cut(s) 395, 859
HpyAV CCTTC 8 cut(s) 272, 449, 1190, 1198, 1320, 1666, 1737, 1903
HpyCH4III ACNGT 6 cut(s) 318, 493, 1417, 1500, 1625, 1903
HpyCH4IV ACGT 1 cut(s) 541
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 1364
HspAI GCGC 2 cut(s) 307, 1364
KasI GGCGCC 1 cut(s) 1363
Kzo9I GATC 5 cut(s) 426, 976, 1629, 1859, 1932
LmnI GCTCC 6 cut(s) 214, 620, 1115, 1252, 1570, 1746
LweI GCATC 8 cut(s) 95, 196, 523, 660, 726, 1170, 1423, 1772
MaeI CTAG 3 cut(s) 678, 872, 1260
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 8 cut(s) 54, 487, 691, 1054, 1426, 1494, 1804, 1961
MalI GATC 5 cut(s) 428, 978, 1631, 1861, 1934
MbiI CCGCTC 1 cut(s) 1165
MboI GATC 5 cut(s) 426, 976, 1629, 1859, 1932
MboII GAAGA 6 cut(s) 931, 934, 1294, 1346, 1631, 1783
MfeI CAATTG 3 cut(s) 264, 288, 786
MflI RGATCY 1 cut(s) 976
MhlI GDGCHC 3 cut(s) 39, 1112, 1737
Mly113I GGCGCC 1 cut(s) 1364
MlyI GAGTC 3 cut(s) 739, 837, 1299
MmeI TCCRAC 1 cut(s) 837
Mph1103I ATGCAT 1 cut(s) 1438
MseI TTAA 9 cut(s) 45, 51, 146, 801, 1082, 1448, 1554, 1668, 1875
MslI CAYNNNNRTG 4 cut(s) 450, 591, 1238, 1485
MspA1I CMGCKG 1 cut(s) 821
MspCI CTTAAG 1 cut(s) 1874
MspI CCGG 3 cut(s) 1001, 1184, 1786
MspR9I CCNGG 5 cut(s) 648, 962, 1680, 1787, 1955
MunI CAATTG 3 cut(s) 264, 288, 786
Mva1269I GAATGC 1 cut(s) 1269
MvaI CCWGG 4 cut(s) 648, 962, 1680, 1955
NarI GGCGCC 1 cut(s) 1364
NciI CCSGG 1 cut(s) 1787
NcoI CCATGG 2 cut(s) 511, 756
NdeI CATATG 1 cut(s) 214
NdeII GATC 5 cut(s) 426, 976, 1629, 1859, 1932
NlaIV GGNNCC 6 cut(s) 1047, 1111, 1365, 1566, 1677, 1761
NmeAIII GCCGAG 1 cut(s) 1738
NmuCI GTSAC 4 cut(s) 54, 1494, 1804, 1961
NsiI ATGCAT 1 cut(s) 1438
NspI RCATGY 3 cut(s) 590, 1484, 1542
NspV TTCGAA 1 cut(s) 2032
PaeR7I CTCGAG 1 cut(s) 1596
PagI TCATGA 1 cut(s) 1308
PaqCI CACCTGC 1 cut(s) 1183
PasI CCCWGGG 1 cut(s) 1679
PceI AGGCCT 1 cut(s) 1200
PciI ACATGT 1 cut(s) 1480
PctI GAATGC 1 cut(s) 1269
PfeI GAWTC 3 cut(s) 1244, 1337, 1868
PflFI GACNNNGTC 1 cut(s) 1967
PflMI CCANNNNNTGG 1 cut(s) 1220
PleI GAGTC 3 cut(s) 739, 837, 1299
PluTI GGCGCC 1 cut(s) 1367
PpsI GAGTC 3 cut(s) 739, 837, 1299
PpuMI RGGWCCY 1 cut(s) 1760
PscI ACATGT 1 cut(s) 1480
PshBI ATTAAT 1 cut(s) 801
PsiI TTATAA 1 cut(s) 1991
Psp124BI GAGCTC 1 cut(s) 1737
Psp5II RGGWCCY 1 cut(s) 1760
Psp6I CCWGG 4 cut(s) 646, 960, 1678, 1953
PspFI CCCAGC 1 cut(s) 1703
PspGI CCWGG 4 cut(s) 646, 960, 1678, 1953
PspN4I GGNNCC 6 cut(s) 1047, 1111, 1365, 1566, 1677, 1761
PspPI GGNCC 8 cut(s) 499, 760, 998, 1003, 1046, 1675, 1760, 1969
PspPPI RGGWCCY 1 cut(s) 1760
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 976
PsyI GACNNNGTC 1 cut(s) 1967
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 4 cut(s) 460, 783, 1414, 1479
RsaNI GTAC 4 cut(s) 459, 782, 1413, 1478
RseI CAYNNNNRTG 4 cut(s) 450, 591, 1238, 1485
SacI GAGCTC 1 cut(s) 1737
SalI GTCGAC 2 cut(s) 392, 1156
SaqAI TTAA 9 cut(s) 45, 51, 146, 801, 1082, 1448, 1554, 1668, 1875
Sau3AI GATC 5 cut(s) 426, 976, 1629, 1859, 1932
Sau96I GGNCC 8 cut(s) 499, 760, 998, 1003, 1046, 1675, 1760, 1969
SchI GAGTC 3 cut(s) 739, 837, 1299
ScrFI CCNGG 5 cut(s) 648, 962, 1680, 1787, 1955
SduI GDGCHC 3 cut(s) 39, 1112, 1737
SfaNI GCATC 8 cut(s) 95, 196, 523, 660, 726, 1170, 1423, 1772
SfcI CTRYAG 1 cut(s) 30
SfoI GGCGCC 1 cut(s) 1365
Sfr274I CTCGAG 1 cut(s) 1596
SfuI TTCGAA 1 cut(s) 2032
SinI GGWCC 4 cut(s) 998, 1046, 1760, 1969
SlaI CTCGAG 1 cut(s) 1596
SmiMI CAYNNNNRTG 4 cut(s) 450, 591, 1238, 1485
SmlI CTYRAG 2 cut(s) 1596, 1874
SmoI CTYRAG 2 cut(s) 1596, 1874
SseBI AGGCCT 1 cut(s) 1200
SsiI CCGC 3 cut(s) 1165, 1372, 1879
SspDI GGCGCC 1 cut(s) 1363
SspI AATATT 1 cut(s) 81
SspMI CTAG 3 cut(s) 678, 872, 1260
SstI GAGCTC 1 cut(s) 1737
StuI AGGCCT 1 cut(s) 1200
StyD4I CCNGG 5 cut(s) 646, 960, 1678, 1785, 1953
StyI CCWWGG 5 cut(s) 243, 511, 756, 1214, 1695
TaaI ACNGT 6 cut(s) 318, 493, 1417, 1500, 1625, 1903
TaiI ACGT 1 cut(s) 544
TaqI TCGA 6 cut(s) 393, 903, 1157, 1293, 1597, 2032
TfiI GAWTC 3 cut(s) 1244, 1337, 1868
Tru1I TTAA 9 cut(s) 45, 51, 146, 801, 1082, 1448, 1554, 1668, 1875
Tru9I TTAA 9 cut(s) 45, 51, 146, 801, 1082, 1448, 1554, 1668, 1875
TscAI CASTG 3 cut(s) 316, 1503, 1979
TseFI GTSAC 4 cut(s) 54, 1494, 1804, 1961
Tsp45I GTSAC 4 cut(s) 54, 1494, 1804, 1961
TspDTI ATGAA 6 cut(s) 309, 468, 932, 1325, 1996, 2001
TspRI CASTG 3 cut(s) 316, 1503, 1979
Tth111I GACNNNGTC 1 cut(s) 1967
Van91I CCANNNNNTGG 1 cut(s) 1220
Vha464I CTTAAG 1 cut(s) 1874
VpaK11BI GGWCC 4 cut(s) 998, 1046, 1760, 1969
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 3 cut(s) 465, 1314, 2003
XceI RCATGY 3 cut(s) 590, 1484, 1542
XcmI CCANNNNNNNNNTGG 1 cut(s) 509
XhoI CTCGAG 1 cut(s) 1596
XmiI GTMKAC 2 cut(s) 393, 1157
XspI CTAG 3 cut(s) 678, 872, 1260
Zsp2I ATGCAT 1 cut(s) 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.