Rroxscaffold_4G00320730

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002846
Physical Location & Seq
Reverse (-)
49586491 .. 49592873
6383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320730.1

Sequence Viewer

Length: 2154 bp
ATGGCGTGGAGACAGCTTACCAAAAAGGGCATACGTGCCGGTCTAAATACTGATTTGACGTCAAGACTACGAAGATATGCAGTGGGATTTACTTCTTTATCTAACGTCGATGATGTAGTAAAATCAGTTTCGTGTACACCAGAGCCCTCATTTAAAGTTAAGTCACGAAAGCTTGTGTGTGGTGAAATATTGATAAAAGACATCCAAATACGATGCTATAATGGGAGAAATGGGAATGACCAGTCATTTGGTTTAAAATCTCCCAAGAGAGAGAAGTTTGTAAAGAGGGACAATAAGGCACAGCCTCCTGTTGATGCTCCATATGTGCCTCCTAAACCACAAAGAACTACCAAGGCCATGCCAGATAAGACAATTGAGATTTTTGAAGGTATGACAATTGATGAACTTGCCAAGCGCACTGGACAGTCAATATCTACTCTGCAAACCATTCTTACAAATGTTGGGGAAAAGGTTGGCTCGGAGTTTGATACTCTCAGCGTCGACATTGCAGAGCTGGTTGCAATGGAAGTTGGGGTCAATGTGAGGAGGCTGCACTCCAATGAAGGTACAGAAATTCTACCACGACCTCCAGTTGTAACTGTCATGGGCCATGTTGACCATGGGAAAACTTCACTTTTGGATGCTCTACGTCAGACATCTGTGGCTGCCAAGGAAGCTGGAGGAATTACTCAGCATGTTGGTGCTTTTGTTGTTGGCATGACATCTGGAGCGTCAATTACATTTCTTGACACCCCTGGTCATGCAGCATTTAGTGCGATGCGAGCTAGAGGGGCTGCTGTTACAGATATAGTTGTGCTTGTTGTAGCTGCTGATGATGGGGTGATGCCTCAGACTCTTGAAGCCATGGCCCATGCAAAGGCAGCTAAAGTACCAATTGTTGTTGCAATTAATAAATGTGATAAACCAGCTGCTAACGCAGAAAAAGTAAGACTCCAGCTTGCGTCGGAGGGCTTGCTGCTAGAGGATATGGGTGGAGATGTTCAGGTTGTTGAGGTTTCAGCTATGAAGAAGACTGGATTAGATAACTTGGAGGAGGCGTTGCTTCTCCAGGCTGAGATGATGGATCTGAAAGCTCGTATTGATGGACCGGCCCAAGCTTATGTGGTGGAAGCAAGACTTGATAGGGGAAAGGGTCCATTGGTTACTGCAATAGTGAAAGCAGGAACTTTGATTTGTGGGAAATATGTGGTCGTGGGCTCCGAGTGGGGTAGAATAAGGGCTATTCGGGACATGGCAGGGAAGTCGACAGAGCAGGCAACACCTGCGATGCCAGTTGAGATTGAAGGCCTGAAGGGACTTCCAAGGGCTGGAGATGATATCATTGTTGTGGAATCTGAGGAGCGAGCTAGAATGCTTAGTGCAGGGAGGAAGAGGAAGTTCGAGAAAGATAGACTTATGAAAATTATGGATGGAAGGGTAGAAGATTCAGAAACAGAACCATCAGATGAGGTGCCTAAGCGGGTTGAATTGCCAATAATAGTAAAAGGGGATGTGCAGGGTACTGTCCAAGCTGTTACAGATGCATTAGGGAGTTTAAATAGTCCTCAGGTTTTTGTGAATGTGGTACATGTTGGTGTTGGGTCGCTATCTCAGTCTGATGTGGACATGGCACAAGCCTGTGGTGCATGTATTATTGGATTTAACATCAAGGCTCCACCCAGTTCCATCAGTTTGGCTGCGTCTCGAGCCAATATCAAGATAATGCAACACCGTGTGATCTATCATCTTCTGGAGGACATTGGGAACTTTATAGTTAAGAAGGCCCCTGGGACTACCGAGACCAAGGTTGCTGGGGAAGCCGAGGTTCTTAGTATTTTTGAGCTCAAAGGAAGGAGTAAGTCTAAGGGACCTGATGTGAAGATTGCCGGATGCCGGGTGGTTGATGGTTTTGTCACTAAATCGGCAACCCTAAGGCTTCTTAGGAGTGGAGAAGTTATATTTGAGGGATCATGTGAATCGCTTAAGCGGGAGAAACAGGATGTGGACACGGTAAAGAAGGGGAATGAGTGTGGACTGGTGATCCAAAATTGCTATGATTTCCAGGTTGGTGACATGGTCCAGTGCTTGCAGCAAGTTATAAGGAAGCCCAAATTCATTTCATCGGAGAGTGGTGCTGTTCGAATAGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU IF-2

Protein Analysis

717

Amino Acids

77.27

Weight (kDa)

8.6

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 199 - 355 3.7e-33 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 199 - 305 1.5e-09 50S ribosome-binding GTPase
FeoB_N PF02421 199 - 353 4.7e-06 Ferrous iron transport protein B
EF-G_D2 PF22042 370 - 449 3.3e-19 Elongation factor G domain 2
IF-2 PF11987 493 - 587 3.4e-28 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2099
AarI CACCTGC 1 cut(s) 1291
AatII GACGTC 1 cut(s) 62
Acc36I ACCTGC 1 cut(s) 1291
AccB1I GGYRCC 1 cut(s) 1471
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccI GTMKAC 2 cut(s) 501, 1265
AciI CCGC 2 cut(s) 1480, 1987
AclWI GGATC 3 cut(s) 1092, 1975, 2035
AcsI RAATTY 2 cut(s) 573, 2111
AcuI CTGAAG 1 cut(s) 1331
AcyI GRCGYC 1 cut(s) 59
AdeI CACNNNGTG 1 cut(s) 1735
AfaI GTAC 5 cut(s) 136, 568, 891, 1522, 1587
AfiI CCNNNNNNNGG 3 cut(s) 877, 1328, 1893
AflII CTTAAG 1 cut(s) 1982
AflIII ACRYGT 1 cut(s) 1588
AgsI TTSAA 4 cut(s) 386, 860, 1304, 1487
AjnI CCWGG 4 cut(s) 754, 1068, 1786, 2061
Alw21I GWGCWC 1 cut(s) 1845
Alw26I GTCTC 3 cut(s) 4, 1707, 1793
AlwI GGATC 3 cut(s) 1092, 1975, 2035
Ama87I CYCGRG 1 cut(s) 1704
AoxI GGCC 6 cut(s) 354, 607, 867, 1110, 1306, 1782
ApoI RAATTY 2 cut(s) 573, 2111
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AseI ATTAAT 1 cut(s) 909
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 8 cut(s) 607, 868, 1106, 1111, 1154, 1783, 1868, 2077
AsuC2I CCSGG 1 cut(s) 1895
AsuHPI GGTGA 4 cut(s) 194, 853, 2050, 2081
AsuII TTCGAA 1 cut(s) 2140
AvaI CYCGRG 1 cut(s) 1704
AvaII GGWCC 4 cut(s) 1106, 1154, 1868, 2077
AxyI CCTNAGG 2 cut(s) 1566, 1931
BanI GGYRCC 1 cut(s) 1471
BanII GRGCYC 3 cut(s) 147, 1220, 1845
BbsI GAAGAC 1 cut(s) 1037
Bbv12I GWGCWC 1 cut(s) 1845
BccI CCATC 7 cut(s) 830, 1075, 1097, 1424, 1468, 1694, 1898
BcgI CGANNNNNNTGC 2 cut(s) 1245, 1279
BciT130I CCWGG 4 cut(s) 756, 1070, 1788, 2063
BcnI CCSGG 1 cut(s) 1895
BcoDI GTCTC 3 cut(s) 4, 1707, 1793
BfaI CTAG 3 cut(s) 786, 980, 1368
BfrI CTTAAG 1 cut(s) 1982
BfuAI ACCTGC 1 cut(s) 1291
Bme1390I CCNGG 5 cut(s) 756, 1070, 1788, 1895, 2063
Bme18I GGWCC 4 cut(s) 1106, 1154, 1868, 2077
BmeT110I CYCGRG 1 cut(s) 1704
BmgT120I GGNCC 8 cut(s) 607, 868, 1106, 1111, 1154, 1783, 1868, 2077
BmiI GGNNCC 6 cut(s) 1155, 1219, 1473, 1674, 1785, 1869
BmrFI CCNGG 5 cut(s) 756, 1070, 1788, 1895, 2063
BmrI ACTGGG 1 cut(s) 1674
BmsI GCATC 8 cut(s) 203, 304, 631, 768, 834, 1278, 1531, 1880
BmuI ACTGGG 1 cut(s) 1674
BpiI GAAGAC 1 cut(s) 1037
BpmI CTGGAG 7 cut(s) 573, 699, 747, 938, 1052, 1350, 1772
Bpu10I CCTNAGC 1 cut(s) 1476
Bpu14I TTCGAA 1 cut(s) 2140
BpuMI CCSGG 1 cut(s) 1895
BsaAI YACGTR 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 1793
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 3 cut(s) 877, 1328, 1893
Bse118I RCCGGY 2 cut(s) 38, 1108
Bse1I ACTGG 8 cut(s) 241, 424, 590, 1039, 1292, 1680, 2040, 2080
Bse21I CCTNAGG 2 cut(s) 1566, 1931
Bse3DI GCAATG 2 cut(s) 504, 528
BseBI CCWGG 4 cut(s) 756, 1070, 1788, 2063
BseGI GGATG 6 cut(s) 201, 646, 1435, 1516, 1895, 2005
BseLI CCNNNNNNNGG 3 cut(s) 877, 1328, 1893
BseMI GCAATG 2 cut(s) 504, 528
BseMII CTCAG 7 cut(s) 508, 704, 863, 1065, 1347, 1580, 1625
BseNI ACTGG 8 cut(s) 241, 424, 590, 1039, 1292, 1680, 2040, 2080
BseRI GAGGAG 3 cut(s) 559, 1067, 1373
BseYI CCCAGC 1 cut(s) 1811
BsgI GTGCAG 3 cut(s) 536, 1401, 1535
BshFI GGCC 6 cut(s) 356, 609, 869, 1112, 1308, 1784
BshNI GGYRCC 1 cut(s) 1471
BsiHKAI GWGCWC 1 cut(s) 1845
BsiHKCI CYCGRG 1 cut(s) 1704
BsiSI CCGG 4 cut(s) 39, 1109, 1887, 1894
BslFI GGGAC 5 cut(s) 302, 1262, 1329, 1804, 1881
BslI CCNNNNNNNGG 3 cut(s) 877, 1328, 1893
BsmAI GTCTC 3 cut(s) 4, 1707, 1793
BsmBI CGTCTC 1 cut(s) 1707
BsmFI GGGAC 5 cut(s) 302, 1262, 1329, 1804, 1881
BsmI GAATGC 1 cut(s) 1377
BsnI GGCC 6 cut(s) 356, 609, 869, 1112, 1308, 1784
Bso31I GGTCTC 1 cut(s) 1793
BsoBI CYCGRG 1 cut(s) 1704
Bsp119I TTCGAA 1 cut(s) 2140
Bsp1286I GDGCHC 3 cut(s) 147, 1220, 1845
Bsp1407I TGTACA 1 cut(s) 134
Bsp143I GATC 4 cut(s) 1084, 1737, 1967, 2040
Bsp19I CCATGG 2 cut(s) 619, 864
BspACI CCGC 2 cut(s) 1480, 1987
BspANI GGCC 6 cut(s) 356, 609, 869, 1112, 1308, 1784
BspCNI CTCAG 7 cut(s) 507, 703, 862, 1066, 1348, 1579, 1624
BspLI GGNNCC 6 cut(s) 1155, 1219, 1473, 1674, 1785, 1869
BspMI ACCTGC 1 cut(s) 1291
BspPI GGATC 3 cut(s) 1092, 1975, 2035
BspT104I TTCGAA 1 cut(s) 2140
BspT107I GGYRCC 1 cut(s) 1471
BspTI CTTAAG 1 cut(s) 1982
BspTNI GGTCTC 1 cut(s) 1793
BsrDI GCAATG 2 cut(s) 504, 528
BsrFI RCCGGY 2 cut(s) 38, 1108
BsrGI TGTACA 1 cut(s) 134
BsrI ACTGG 8 cut(s) 241, 424, 590, 1039, 1292, 1680, 2040, 2080
BssAI RCCGGY 2 cut(s) 38, 1108
BssMI GATC 4 cut(s) 1084, 1737, 1967, 2040
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 6 cut(s) 351, 619, 669, 864, 1322, 1803
Bst2UI CCWGG 4 cut(s) 756, 1070, 1788, 2063
Bst4CI ACNGT 5 cut(s) 426, 601, 1525, 1733, 2011
Bst6I CTCTTC 1 cut(s) 1385
BstACI GRCGYC 1 cut(s) 59
BstAFI CTTAAG 1 cut(s) 1982
BstAPI GCANNNNNTGC 2 cut(s) 773, 1283
BstAUI TGTACA 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 2140
BstC8I GCNNGC 6 cut(s) 783, 960, 974, 1275, 1365, 2087
BstDSI CCRYGG 2 cut(s) 619, 864
BstF5I GGATG 6 cut(s) 201, 646, 1435, 1516, 1895, 2005
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 4 cut(s) 1087, 1740, 1970, 2043
BstMAI GTCTC 3 cut(s) 4, 1707, 1793
BstMBI GATC 4 cut(s) 1084, 1737, 1967, 2040
BstNI CCWGG 4 cut(s) 756, 1070, 1788, 2063
BstNSI RCATGY 3 cut(s) 698, 1592, 1650
BstSCI CCNGG 5 cut(s) 754, 1068, 1786, 1893, 2061
BstV2I GAAGAC 1 cut(s) 1037
BstX2I RGATCY 1 cut(s) 1084
BstXI CCANNNNNNTGG 2 cut(s) 248, 1693
BstYI RGATCY 1 cut(s) 1084
Bsu36I CCTNAGG 2 cut(s) 1566, 1931
BsuRI GGCC 6 cut(s) 356, 609, 869, 1112, 1308, 1784
BtgI CCRYGG 2 cut(s) 619, 864
BtgZI GCGATG 2 cut(s) 791, 1301
BtsCI GGATG 6 cut(s) 201, 646, 1435, 1516, 1895, 2005
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 87, 417, 2087
BveI ACCTGC 1 cut(s) 1291
Cac8I GCNNGC 6 cut(s) 783, 960, 974, 1275, 1365, 2087
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 2 cut(s) 38, 1108
Cfr13I GGNCC 8 cut(s) 607, 868, 1106, 1111, 1154, 1783, 1868, 2077
CseI GACGC 4 cut(s) 487, 720, 951, 1689
Csp6I GTAC 5 cut(s) 135, 567, 890, 1521, 1586
CviQI GTAC 5 cut(s) 135, 567, 890, 1521, 1586
DpnI GATC 4 cut(s) 1086, 1739, 1969, 2042
DpnII GATC 4 cut(s) 1084, 1737, 1967, 2040
DraI TTTAAA 3 cut(s) 154, 255, 1557
DraIII CACNNNGTG 1 cut(s) 1735
Eam1104I CTCTTC 1 cut(s) 1385
EarI CTCTTC 1 cut(s) 1385
Ecl136II GAGCTC 1 cut(s) 1843
Eco130I CCWWGG 6 cut(s) 351, 619, 669, 864, 1322, 1803
Eco147I AGGCCT 1 cut(s) 1308
Eco24I GRGCYC 3 cut(s) 147, 1220, 1845
Eco31I GGTCTC 1 cut(s) 1793
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 4 cut(s) 1106, 1154, 1868, 2077
Eco53kI GAGCTC 1 cut(s) 1843
Eco57I CTGAAG 1 cut(s) 1331
Eco81I CCTNAGG 2 cut(s) 1566, 1931
Eco88I CYCGRG 1 cut(s) 1704
EcoICRI GAGCTC 1 cut(s) 1843
EcoO109I RGGNCCY 2 cut(s) 1783, 1868
EcoRII CCWGG 4 cut(s) 754, 1068, 1786, 2061
EcoRV GATATC 1 cut(s) 1339
EcoT14I CCWWGG 6 cut(s) 351, 619, 669, 864, 1322, 1803
EcoT22I ATGCAT 1 cut(s) 1546
EcoT38I GRGCYC 3 cut(s) 147, 1220, 1845
ErhI CCWWGG 6 cut(s) 351, 619, 669, 864, 1322, 1803
Esp3I CGTCTC 1 cut(s) 1707
FalI AAGNNNNNCTT 4 cut(s) 1122, 1154, 1398, 1430
FaqI GGGAC 5 cut(s) 302, 1262, 1329, 1804, 1881
FauI CCCGC 2 cut(s) 1473, 1980
FauNDI CATATG 1 cut(s) 322
FblI GTMKAC 2 cut(s) 501, 1265
FokI GGATG 6 cut(s) 188, 653, 1442, 1523, 1902, 2012
FriOI GRGCYC 3 cut(s) 147, 1220, 1845
FspBI CTAG 3 cut(s) 786, 980, 1368
GlaI GCGC 1 cut(s) 416
GsaI CCCAGC 1 cut(s) 1815
GsuI CTGGAG 7 cut(s) 573, 699, 747, 938, 1052, 1350, 1772
HaeIII GGCC 6 cut(s) 356, 609, 869, 1112, 1308, 1784
HapII CCGG 4 cut(s) 39, 1109, 1887, 1894
HgaI GACGC 4 cut(s) 487, 720, 951, 1689
HhaI GCGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 59
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 3 cut(s) 502, 616, 1266
HindII GTYRAC 3 cut(s) 502, 616, 1266
HindIII AAGCTT 2 cut(s) 170, 1116
HinfI GANTC 5 cut(s) 853, 951, 1352, 1445, 1976
HpaII CCGG 4 cut(s) 39, 1109, 1887, 1894
HphI GGTGA 4 cut(s) 194, 853, 2050, 2081
Hpy166II GTNNAC 8 cut(s) 135, 137, 502, 616, 1266, 1624, 2005, 2033
Hpy8I GTNNAC 8 cut(s) 135, 137, 502, 616, 1266, 1624, 2005, 2033
Hpy99I CGWCG 3 cut(s) 110, 503, 967
HpyAV CCTTC 8 cut(s) 380, 557, 1298, 1306, 1428, 1774, 1845, 2011
HpyCH4III ACNGT 5 cut(s) 426, 601, 1525, 1733, 2011
HpyCH4IV ACGT 4 cut(s) 34, 59, 105, 649
HpySE526I ACGT 4 cut(s) 34, 59, 105, 649
Hsp92I GRCGYC 1 cut(s) 59
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 4 cut(s) 1084, 1737, 1967, 2040
LmnI GCTCC 5 cut(s) 322, 728, 1223, 1360, 1678
LweI GCATC 8 cut(s) 203, 304, 631, 768, 834, 1278, 1531, 1880
MaeI CTAG 3 cut(s) 786, 980, 1368
MaeII ACGT 4 cut(s) 34, 59, 105, 649
MaeIII GTNAC 7 cut(s) 162, 595, 799, 1162, 1534, 1912, 2069
MalI GATC 4 cut(s) 1086, 1739, 1969, 2042
MboI GATC 4 cut(s) 1084, 1737, 1967, 2040
MboII GAAGA 7 cut(s) 84, 1039, 1042, 1402, 1454, 1739, 1891
MfeI CAATTG 3 cut(s) 372, 396, 894
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 3 cut(s) 147, 1220, 1845
MlyI GAGTC 2 cut(s) 847, 945
MmeI TCCRAC 1 cut(s) 945
Mph1103I ATGCAT 1 cut(s) 1546
MseI TTAA 8 cut(s) 153, 159, 254, 909, 1556, 1662, 1776, 1983
MslI CAYNNNNRTG 4 cut(s) 558, 699, 1346, 1593
MspA1I CMGCKG 1 cut(s) 929
MspCI CTTAAG 1 cut(s) 1982
MspI CCGG 4 cut(s) 39, 1109, 1887, 1894
MspR9I CCNGG 5 cut(s) 756, 1070, 1788, 1895, 2063
MunI CAATTG 3 cut(s) 372, 396, 894
Mva1269I GAATGC 1 cut(s) 1377
MvaI CCWGG 4 cut(s) 756, 1070, 1788, 2063
NciI CCSGG 1 cut(s) 1895
NcoI CCATGG 2 cut(s) 619, 864
NdeI CATATG 1 cut(s) 322
NdeII GATC 4 cut(s) 1084, 1737, 1967, 2040
NlaIV GGNNCC 6 cut(s) 1155, 1219, 1473, 1674, 1785, 1869
NmeAIII GCCGAG 1 cut(s) 1846
NmuCI GTSAC 3 cut(s) 162, 1912, 2069
NsiI ATGCAT 1 cut(s) 1546
NspI RCATGY 3 cut(s) 698, 1592, 1650
NspV TTCGAA 1 cut(s) 2140
PaeR7I CTCGAG 1 cut(s) 1704
PaqCI CACCTGC 1 cut(s) 1291
PasI CCCWGGG 1 cut(s) 1787
PceI AGGCCT 1 cut(s) 1308
PciI ACATGT 1 cut(s) 1588
PcsI WCGNNNNNNNCGW 1 cut(s) 1218
PctI GAATGC 1 cut(s) 1377
PfeI GAWTC 3 cut(s) 1352, 1445, 1976
PflFI GACNNNGTC 1 cut(s) 2075
PflMI CCANNNNNTGG 1 cut(s) 1328
PleI GAGTC 2 cut(s) 847, 945
PpsI GAGTC 2 cut(s) 847, 945
Ppu21I YACGTR 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 1868
PscI ACATGT 1 cut(s) 1588
PshBI ATTAAT 1 cut(s) 909
PsiI TTATAA 1 cut(s) 2099
Psp124BI GAGCTC 1 cut(s) 1845
Psp5II RGGWCCY 1 cut(s) 1868
Psp6I CCWGG 4 cut(s) 754, 1068, 1786, 2061
PspFI CCCAGC 1 cut(s) 1811
PspGI CCWGG 4 cut(s) 754, 1068, 1786, 2061
PspN4I GGNNCC 6 cut(s) 1155, 1219, 1473, 1674, 1785, 1869
PspPI GGNCC 8 cut(s) 607, 868, 1106, 1111, 1154, 1783, 1868, 2077
PspPPI RGGWCCY 1 cut(s) 1868
PsuI RGATCY 1 cut(s) 1084
PsyI GACNNNGTC 1 cut(s) 2075
PvuII CAGCTG 1 cut(s) 929
RsaI GTAC 5 cut(s) 136, 568, 891, 1522, 1587
RsaNI GTAC 5 cut(s) 135, 567, 890, 1521, 1586
RseI CAYNNNNRTG 4 cut(s) 558, 699, 1346, 1593
SacI GAGCTC 1 cut(s) 1845
SalI GTCGAC 2 cut(s) 500, 1264
SaqAI TTAA 8 cut(s) 153, 159, 254, 909, 1556, 1662, 1776, 1983
Sau3AI GATC 4 cut(s) 1084, 1737, 1967, 2040
Sau96I GGNCC 8 cut(s) 607, 868, 1106, 1111, 1154, 1783, 1868, 2077
SchI GAGTC 2 cut(s) 847, 945
ScrFI CCNGG 5 cut(s) 756, 1070, 1788, 1895, 2063
SduI GDGCHC 3 cut(s) 147, 1220, 1845
SfaNI GCATC 8 cut(s) 203, 304, 631, 768, 834, 1278, 1531, 1880
Sfr274I CTCGAG 1 cut(s) 1704
SfuI TTCGAA 1 cut(s) 2140
SinI GGWCC 4 cut(s) 1106, 1154, 1868, 2077
SlaI CTCGAG 1 cut(s) 1704
SmiMI CAYNNNNRTG 4 cut(s) 558, 699, 1346, 1593
SmlI CTYRAG 2 cut(s) 1704, 1982
SmoI CTYRAG 2 cut(s) 1704, 1982
SseBI AGGCCT 1 cut(s) 1308
SsiI CCGC 2 cut(s) 1480, 1987
SspI AATATT 1 cut(s) 189
SspMI CTAG 3 cut(s) 786, 980, 1368
SstI GAGCTC 1 cut(s) 1845
StuI AGGCCT 1 cut(s) 1308
StyD4I CCNGG 5 cut(s) 754, 1068, 1786, 1893, 2061
StyI CCWWGG 6 cut(s) 351, 619, 669, 864, 1322, 1803
TaaI ACNGT 5 cut(s) 426, 601, 1525, 1733, 2011
TaiI ACGT 4 cut(s) 37, 62, 108, 652
TaqI TCGA 6 cut(s) 108, 501, 1265, 1401, 1705, 2140
TatI WGTACW 1 cut(s) 134
TfiI GAWTC 3 cut(s) 1352, 1445, 1976
Tru1I TTAA 8 cut(s) 153, 159, 254, 909, 1556, 1662, 1776, 1983
Tru9I TTAA 8 cut(s) 153, 159, 254, 909, 1556, 1662, 1776, 1983
TscAI CASTG 3 cut(s) 87, 424, 2087
TseFI GTSAC 3 cut(s) 162, 1912, 2069
Tsp45I GTSAC 3 cut(s) 162, 1912, 2069
TspDTI ATGAA 6 cut(s) 417, 576, 1040, 1433, 2104, 2109
TspRI CASTG 3 cut(s) 87, 424, 2087
Tth111I GACNNNGTC 1 cut(s) 2075
Van91I CCANNNNNTGG 1 cut(s) 1328
Vha464I CTTAAG 1 cut(s) 1982
VpaK11BI GGWCC 4 cut(s) 1106, 1154, 1868, 2077
VspI ATTAAT 1 cut(s) 909
XapI RAATTY 2 cut(s) 573, 2111
XceI RCATGY 3 cut(s) 698, 1592, 1650
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XhoI CTCGAG 1 cut(s) 1704
XmiI GTMKAC 2 cut(s) 501, 1265
XspI CTAG 3 cut(s) 786, 980, 1368
ZraI GACGTC 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.