Rroxscaffold_3G00260210

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002846
Physical Location & Seq
Reverse (-)
54655245 .. 54669517
14273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260210.1

Sequence Viewer

Length: 2367 bp
ATGAGTAAGGACACTACCGGTGAAAGCGAGCAAAGCCAGGCTCACCGACCTAAACTGCTCGTCGCCGACGAACTGTACCCTGTTCTCCTCTTAAACACTAATTCTGATAATTTCTCAACTGCTATTCATCTCTATGTTCTGGGTTTACTTCTCCCTTTGAGAAAACTCATTCAGTTTGGTCTAAGTTGTCCGGGTTTCTTATTGACCATGCCATTTCCTAAGAATGACATTTTGTCATTGGGCATATGTGCCAGTTTAAATACTGATTTGACTTCAAGACTACGAAGATATGCAGTGGGATTTACTTCTTTATCTAATGTTGATGATGTAGTAAAATCAGTTTTGTGTACACCAGAGCCCTCATTTAAATTTAAGTTACGAAAGCTTGTTTATGGTGAAATATTGATAAAAGACACCCAAATACGATGCTATCATGGGAGAAAAGGGAATGACCAGTCATTTGGTTTAAAACCTCCCAAGAGAGAGAAGTTTGTAAAGAGGGACAATAAGACACAGCCTCCTGTTGATGCTCCATATGTGCCTCCTAAACCACAAAGAACTACCAAGGCCATGCCAGATAAGACAATTGAGATTTTTGAAGGTATGACAATTGATGAACTTGCCAAGCGCACTGGACAGTCAATATCTACTCTGCAAACCATTCTTACAAATGTTGGGGAAAAGGTTGGCTCGGAGTTTGACACTCTCAGCATCGACATTGCAGAGCTGGTTGCAATGGAAGTTGGGGTCAATGTGAGGAGGCTGCACTCCAATGAAGGTACACAAATTCTACCACGACCCCCAGTTGTAACTGTCATGGGCCATGTTGACCATGGGAAAACTTCACTTTTGGATGCTCTACGTCAGACATCTGTGGCTGCCAAAGAAGCTGGAGGGATTACTCAGCATGTTGGTGCTTTTGTTGTTGGCATGACATCTGGAGCATCAATTACTTTTCTTGACACCCCTGGTCATGCAGCATTTAGTGCGATGCGAGCTAGAGGAGCTGCTGTCACAGATATAGTTGTGCTTGTTGTAGCTGCTGATGACGGGGTGATGCCTCAGACTCTTGAAGCCATGGCCCATGCAAAGGCAGCTAAAGTACCAATTGTTGTTGCAATTAATAAATGTGATAAACCAGCTGCTAACGCAGAAAAGGTAAGACTCCAGCTTGCGTCGGAGGGCTTGCTGCTAGAGGATATGGGTGGGGATGTTCAGGTTGTTGAGGTTTCAGCTATGAAGAAGACTGGTTTAGATAACTTGGAGGAGGCTTTGCTTCTCCAGGCTGAGATGATGGATCTGAAAGCTCGTATTGATGGACCGGCTCAAGCTTATGTGGTGGAAGCAAGACTTGATAGGGGAAAGGGTCCATTGGTTACTGCAATAGTGAAAGCAGGAACTTTAATTTGTGGGAAATATGTTGTCGTGGGCTCTGAGTGGGGTAGAATAAGGGCTATTAGGGACATGGCAGGGAAGTCGACAGAGCGGGTAACACCTGCTATGCCAGTTGAGATTGAAGGCCTGAAGGGACTTCCAAGGGCTGGAGATGATATCATTGTTGTGGAATCTGAGGAGCGAGCTAGAATGCTTAGTGCAGGGAGGAAAAGGAAGTTCGAGAAAGATAGACTCATGAAAATTATGGATGGAAGGGTAGAAGATCCAGAAACAGAACCATCAGATGAGGCGCCCAAGCGGGTTGAATTGCCGATAATAGTAAAAGGGGATGTGCAGGGTACTGTCCAAGCTGTTACAGATGCATTAGGGAGTTTAAATAGTCCTCAGGTTTTTGTGAATGTGGTACATGTTGGTGTTGGGTCGCTATCTCAGTCTGATGTGGACATGGCACAAGCCTGTGGTGCATGTATTATTGGATTTAACATCAAGTCTCCACCCAGTTCCATCAGTTTGACTGCATCTCGAGCCAATATCAAGATAATGCAACACCGTGTGATCTATCATCTTTTGGAGGACATTGGGAACTTTATAGTTGAGAAGGCCCCTGGGACTACTGAGACCAAGGTTGCTGGGGAAGCCGAGGTTCTTAGTATTTTTGAGCTCAAAGGAAGGAGCAAGTCTAAGGGACCTGATGTGAAGATTGCCGGATGCCGGGTGGTTGATGGTTTTGTCACCAAATCGGCAACCCTGAGGCTTCTTAGGAGTGGGGAAGTTGTATTTGAGGGATCATGTGAATCGCTCAAGCGGGAGAAACAGGATGTGGACACAGTAAAGAAGGGGAGTGAGTGTGGACTGGTGATCCAAAATTGCTACGATTTCCAGGTTGGTGACATGGTCCAGTGCTTACAGCAAGTTATAAGGAAGCCCAAATTCATTTCATCGGAGAGTGGTGCTGTTCGAATAGAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU IF-2

Protein Analysis

788

Amino Acids

85.27

Weight (kDa)

8.06

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 270 - 426 4.4e-33 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 270 - 376 1.8e-09 50S ribosome-binding GTPase
FeoB_N PF02421 270 - 424 5.5e-06 Ferrous iron transport protein B
EF-G_D2 PF22042 441 - 520 1.5e-18 Elongation factor G domain 2
IF-2 PF11987 564 - 658 8.4e-28 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2312
AarI CACCTGC 1 cut(s) 1504
Acc36I ACCTGC 1 cut(s) 1504
AccB1I GGYRCC 1 cut(s) 1684
AccB7I CCANNNNNTGG 1 cut(s) 1541
AccBSI CCGCTC 1 cut(s) 1486
AccI GTMKAC 1 cut(s) 1478
AciI CCGC 3 cut(s) 1486, 1693, 2200
AclWI GGATC 4 cut(s) 1305, 1652, 2188, 2248
AcsI RAATTY 3 cut(s) 368, 786, 2324
AcuI CTGAAG 1 cut(s) 1544
AcyI GRCGYC 1 cut(s) 1685
AdeI CACNNNGTG 1 cut(s) 1948
AfaI GTAC 6 cut(s) 77, 349, 781, 1104, 1735, 1800
AfiI CCNNNNNNNGG 3 cut(s) 1090, 1541, 2106
AflIII ACRYGT 1 cut(s) 1801
AgeI ACCGGT 1 cut(s) 17
AgsI TTSAA 5 cut(s) 276, 599, 1073, 1517, 1700
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 5 cut(s) 36, 967, 1281, 1999, 2274
Alw21I GWGCWC 1 cut(s) 2058
Alw26I GTCTC 2 cut(s) 1890, 2006
AlwI GGATC 4 cut(s) 1305, 1652, 2188, 2248
Ama87I CYCGRG 1 cut(s) 1917
AoxI GGCC 5 cut(s) 567, 820, 1080, 1519, 1995
ApeKI GCWGC 8 cut(s) 763, 878, 977, 1007, 1040, 1094, 1142, 1189
ApoI RAATTY 3 cut(s) 368, 786, 2324
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
AseI ATTAAT 1 cut(s) 1122
AsiGI ACCGGT 1 cut(s) 17
AspLEI GCGC 2 cut(s) 630, 1687
AspS9I GGNCC 7 cut(s) 820, 1081, 1319, 1367, 1996, 2081, 2290
AsuC2I CCSGG 2 cut(s) 192, 2108
AsuHPI GGTGA 7 cut(s) 32, 35, 407, 1066, 2119, 2263, 2294
AsuII TTCGAA 1 cut(s) 2353
AvaI CYCGRG 1 cut(s) 1917
AvaII GGWCC 4 cut(s) 1319, 1367, 2081, 2290
AxyI CCTNAGG 2 cut(s) 1779, 2144
BanI GGYRCC 1 cut(s) 1684
BanII GRGCYC 3 cut(s) 360, 1433, 2058
BbsI GAAGAC 1 cut(s) 1250
Bbv12I GWGCWC 1 cut(s) 2058
BbvI GCAGC 8 cut(s) 750, 865, 989, 994, 1027, 1106, 1129, 1176
BccI CCATC 6 cut(s) 1288, 1310, 1637, 1681, 1907, 2111
BcgI CGANNNNNNTGC 2 cut(s) 1458, 1492
BciT130I CCWGG 5 cut(s) 38, 969, 1283, 2001, 2276
BcnI CCSGG 2 cut(s) 192, 2108
BcoDI GTCTC 2 cut(s) 1890, 2006
BfaI CTAG 3 cut(s) 999, 1193, 1581
BfoI RGCGCY 1 cut(s) 1688
BfuAI ACCTGC 1 cut(s) 1504
BisI GCNGC 8 cut(s) 764, 879, 978, 1008, 1041, 1095, 1143, 1190
BlsI GCNGC 8 cut(s) 765, 880, 979, 1009, 1042, 1096, 1144, 1191
Bme1390I CCNGG 7 cut(s) 38, 192, 969, 1283, 2001, 2108, 2276
Bme18I GGWCC 4 cut(s) 1319, 1367, 2081, 2290
BmeRI GACNNNNNGTC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 1917
BmgT120I GGNCC 7 cut(s) 820, 1081, 1319, 1367, 1996, 2081, 2290
BmiI GGNNCC 4 cut(s) 1368, 1686, 1998, 2082
BmrFI CCNGG 7 cut(s) 38, 192, 969, 1283, 2001, 2108, 2276
BmrI ACTGGG 2 cut(s) 797, 1887
BmuI ACTGGG 2 cut(s) 797, 1887
BpiI GAAGAC 1 cut(s) 1250
BpmI CTGGAG 5 cut(s) 912, 960, 1151, 1265, 1563
Bpu14I TTCGAA 1 cut(s) 2353
BpuEI CTTGAG 2 cut(s) 1311, 2180
BpuMI CCSGG 2 cut(s) 192, 2108
BsaHI GRCGYC 1 cut(s) 1685
BsaI GGTCTC 1 cut(s) 2006
BsaJI CCNNGG 9 cut(s) 564, 832, 967, 1077, 1535, 1999, 2000, 2016, 2034
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 6 cut(s) 502, 532, 996, 1026, 2227, 2257
Bsc4I CCNNNNNNNGG 3 cut(s) 1090, 1541, 2106
Bse118I RCCGGY 2 cut(s) 17, 1321
Bse1I ACTGG 9 cut(s) 252, 454, 637, 803, 1252, 1505, 1893, 2253, 2293
Bse21I CCTNAGG 2 cut(s) 1779, 2144
Bse3DI GCAATG 2 cut(s) 717, 741
BseBI CCWGG 5 cut(s) 38, 969, 1283, 2001, 2276
BseDI CCNNGG 9 cut(s) 564, 832, 967, 1077, 1535, 1999, 2000, 2016, 2034
BseGI GGATG 6 cut(s) 859, 1216, 1648, 1729, 2108, 2218
BseLI CCNNNNNNNGG 3 cut(s) 1090, 1541, 2106
BseMI GCAATG 2 cut(s) 717, 741
BseNI ACTGG 9 cut(s) 252, 454, 637, 803, 1252, 1505, 1893, 2253, 2293
BseRI GAGGAG 5 cut(s) 77, 772, 1017, 1280, 1586
BseXI GCAGC 8 cut(s) 750, 865, 989, 994, 1027, 1106, 1129, 1176
BseYI CCCAGC 1 cut(s) 2024
BsgI GTGCAG 3 cut(s) 749, 1614, 1748
BshFI GGCC 5 cut(s) 569, 822, 1082, 1521, 1997
BshNI GGYRCC 1 cut(s) 1684
BshTI ACCGGT 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 2058
BsiHKCI CYCGRG 1 cut(s) 1917
BsiSI CCGG 5 cut(s) 18, 191, 1322, 2100, 2107
BslFI GGGAC 5 cut(s) 515, 1475, 1542, 2017, 2094
BslI CCNNNNNNNGG 3 cut(s) 1090, 1541, 2106
BsmAI GTCTC 2 cut(s) 1890, 2006
BsmFI GGGAC 5 cut(s) 515, 1475, 1542, 2017, 2094
BsmI GAATGC 1 cut(s) 1590
BsnI GGCC 5 cut(s) 569, 822, 1082, 1521, 1997
Bso31I GGTCTC 1 cut(s) 2006
BsoBI CYCGRG 1 cut(s) 1917
Bsp119I TTCGAA 1 cut(s) 2353
Bsp1286I GDGCHC 3 cut(s) 360, 1433, 2058
Bsp1407I TGTACA 1 cut(s) 347
Bsp143I GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
Bsp19I CCATGG 2 cut(s) 832, 1077
BspACI CCGC 3 cut(s) 1486, 1693, 2200
BspANI GGCC 5 cut(s) 569, 822, 1082, 1521, 1997
BspHI TCATGA 1 cut(s) 1629
BspLI GGNNCC 4 cut(s) 1368, 1686, 1998, 2082
BspMI ACCTGC 1 cut(s) 1504
BspPI GGATC 4 cut(s) 1305, 1652, 2188, 2248
BspT104I TTCGAA 1 cut(s) 2353
BspT107I GGYRCC 1 cut(s) 1684
BspTNI GGTCTC 1 cut(s) 2006
BsrBI CCGCTC 1 cut(s) 1486
BsrDI GCAATG 2 cut(s) 717, 741
BsrFI RCCGGY 2 cut(s) 17, 1321
BsrGI TGTACA 1 cut(s) 347
BsrI ACTGG 9 cut(s) 252, 454, 637, 803, 1252, 1505, 1893, 2253, 2293
BssAI RCCGGY 2 cut(s) 17, 1321
BssECI CCNNGG 9 cut(s) 564, 832, 967, 1077, 1535, 1999, 2000, 2016, 2034
BssMI GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
BssNI GRCGYC 1 cut(s) 1685
BssT1I CCWWGG 5 cut(s) 564, 832, 1077, 1535, 2016
Bst2UI CCWGG 5 cut(s) 38, 969, 1283, 2001, 2276
Bst4CI ACNGT 6 cut(s) 75, 639, 814, 1738, 1946, 2224
BstACI GRCGYC 1 cut(s) 1685
BstAPI GCANNNNNTGC 1 cut(s) 986
BstAUI TGTACA 1 cut(s) 347
BstBI TTCGAA 1 cut(s) 2353
BstC8I GCNNGC 5 cut(s) 29, 996, 1173, 1187, 1578
BstDSI CCRYGG 2 cut(s) 832, 1077
BstF5I GGATG 6 cut(s) 859, 1216, 1648, 1729, 2108, 2218
BstH2I RGCGCY 1 cut(s) 1688
BstHHI GCGC 2 cut(s) 630, 1687
BstKTI GATC 5 cut(s) 1300, 1660, 1953, 2183, 2256
BstMAI GTCTC 2 cut(s) 1890, 2006
BstMBI GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
BstNI CCWGG 5 cut(s) 38, 969, 1283, 2001, 2276
BstNSI RCATGY 3 cut(s) 911, 1805, 1863
BstSCI CCNGG 7 cut(s) 36, 190, 967, 1281, 1999, 2106, 2274
BstV1I GCAGC 8 cut(s) 750, 865, 989, 994, 1027, 1106, 1129, 1176
BstV2I GAAGAC 1 cut(s) 1250
BstX2I RGATCY 2 cut(s) 1297, 1657
BstXI CCANNNNNNTGG 1 cut(s) 461
BstYI RGATCY 2 cut(s) 1297, 1657
Bsu36I CCTNAGG 2 cut(s) 1779, 2144
BsuRI GGCC 5 cut(s) 569, 822, 1082, 1521, 1997
BtgI CCRYGG 2 cut(s) 832, 1077
BtgZI GCGATG 1 cut(s) 1004
BtsCI GGATG 6 cut(s) 859, 1216, 1648, 1729, 2108, 2218
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 3 cut(s) 300, 630, 2300
BveI ACCTGC 1 cut(s) 1504
Cac8I GCNNGC 5 cut(s) 29, 996, 1173, 1187, 1578
CciI TCATGA 1 cut(s) 1629
CfoI GCGC 2 cut(s) 630, 1687
Cfr10I RCCGGY 2 cut(s) 17, 1321
Cfr13I GGNCC 7 cut(s) 820, 1081, 1319, 1367, 1996, 2081, 2290
CseI GACGC 1 cut(s) 1164
Csp6I GTAC 6 cut(s) 76, 348, 780, 1103, 1734, 1799
CspAI ACCGGT 1 cut(s) 17
CviQI GTAC 6 cut(s) 76, 348, 780, 1103, 1734, 1799
DinI GGCGCC 1 cut(s) 1686
DpnI GATC 5 cut(s) 1299, 1659, 1952, 2182, 2255
DpnII GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
DraI TTTAAA 4 cut(s) 258, 367, 468, 1770
DraIII CACNNNGTG 1 cut(s) 1948
DriI GACNNNNNGTC 1 cut(s) 232
Eam1105I GACNNNNNGTC 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 2056
Eco130I CCWWGG 5 cut(s) 564, 832, 1077, 1535, 2016
Eco147I AGGCCT 1 cut(s) 1521
Eco24I GRGCYC 3 cut(s) 360, 1433, 2058
Eco31I GGTCTC 1 cut(s) 2006
Eco32I GATATC 1 cut(s) 1552
Eco47I GGWCC 4 cut(s) 1319, 1367, 2081, 2290
Eco53kI GAGCTC 1 cut(s) 2056
Eco57I CTGAAG 1 cut(s) 1544
Eco81I CCTNAGG 2 cut(s) 1779, 2144
Eco88I CYCGRG 1 cut(s) 1917
EcoICRI GAGCTC 1 cut(s) 2056
EcoO109I RGGNCCY 2 cut(s) 1996, 2081
EcoRII CCWGG 5 cut(s) 36, 967, 1281, 1999, 2274
EcoRV GATATC 1 cut(s) 1552
EcoT14I CCWWGG 5 cut(s) 564, 832, 1077, 1535, 2016
EcoT22I ATGCAT 1 cut(s) 1759
EcoT38I GRGCYC 3 cut(s) 360, 1433, 2058
EgeI GGCGCC 1 cut(s) 1686
EheI GGCGCC 1 cut(s) 1686
ErhI CCWWGG 5 cut(s) 564, 832, 1077, 1535, 2016
FalI AAGNNNNNCTT 2 cut(s) 1335, 1367
FaqI GGGAC 5 cut(s) 515, 1475, 1542, 2017, 2094
FauI CCCGC 3 cut(s) 1479, 1686, 2193
FauNDI CATATG 2 cut(s) 245, 535
FblI GTMKAC 1 cut(s) 1478
Fnu4HI GCNGC 8 cut(s) 764, 879, 978, 1008, 1041, 1095, 1143, 1190
FokI GGATG 6 cut(s) 866, 1223, 1655, 1736, 2115, 2225
FriOI GRGCYC 3 cut(s) 360, 1433, 2058
Fsp4HI GCNGC 8 cut(s) 764, 879, 978, 1008, 1041, 1095, 1143, 1190
FspBI CTAG 3 cut(s) 999, 1193, 1581
GlaI GCGC 2 cut(s) 629, 1686
GluI GCNGC 8 cut(s) 764, 879, 978, 1008, 1041, 1095, 1143, 1190
GsaI CCCAGC 1 cut(s) 2028
GsuI CTGGAG 5 cut(s) 912, 960, 1151, 1265, 1563
HaeII RGCGCY 1 cut(s) 1688
HaeIII GGCC 5 cut(s) 569, 822, 1082, 1521, 1997
HapII CCGG 5 cut(s) 18, 191, 1322, 2100, 2107
HgaI GACGC 1 cut(s) 1164
HhaI GCGC 2 cut(s) 630, 1687
Hin1I GRCGYC 1 cut(s) 1685
Hin6I GCGC 2 cut(s) 628, 1685
HinP1I GCGC 2 cut(s) 628, 1685
HincII GTYRAC 2 cut(s) 829, 1479
HindII GTYRAC 2 cut(s) 829, 1479
HindIII AAGCTT 2 cut(s) 383, 1329
HinfI GANTC 5 cut(s) 1066, 1164, 1565, 1626, 2189
HpaII CCGG 5 cut(s) 18, 191, 1322, 2100, 2107
HphI GGTGA 7 cut(s) 32, 35, 407, 1066, 2119, 2263, 2294
Hpy166II GTNNAC 9 cut(s) 146, 348, 350, 782, 829, 1479, 1837, 2218, 2246
Hpy188III TCNNGA 9 cut(s) 276, 939, 959, 1070, 1615, 1630, 1661, 1917, 1930
Hpy8I GTNNAC 9 cut(s) 146, 348, 350, 782, 829, 1479, 1837, 2218, 2246
Hpy99I CGWCG 3 cut(s) 65, 71, 1180
HpyAV CCTTC 8 cut(s) 593, 770, 1511, 1519, 1641, 1987, 2058, 2224
HpyCH4III ACNGT 6 cut(s) 75, 639, 814, 1738, 1946, 2224
HpyCH4IV ACGT 1 cut(s) 862
HpySE526I ACGT 1 cut(s) 862
Hsp92I GRCGYC 1 cut(s) 1685
HspAI GCGC 2 cut(s) 628, 1685
KasI GGCGCC 1 cut(s) 1684
Kzo9I GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
LmnI GCTCC 5 cut(s) 535, 941, 1004, 1573, 2067
Lsp1109I GCAGC 8 cut(s) 750, 865, 989, 994, 1027, 1106, 1129, 1176
MaeI CTAG 3 cut(s) 999, 1193, 1581
MaeII ACGT 1 cut(s) 862
MaeIII GTNAC 8 cut(s) 375, 808, 1012, 1375, 1489, 1747, 2125, 2282
MalI GATC 5 cut(s) 1299, 1659, 1952, 2182, 2255
MbiI CCGCTC 1 cut(s) 1486
MboI GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
MboII GAAGA 5 cut(s) 297, 1252, 1255, 1667, 2104
MfeI CAATTG 3 cut(s) 585, 609, 1107
MflI RGATCY 2 cut(s) 1297, 1657
MhlI GDGCHC 3 cut(s) 360, 1433, 2058
Mly113I GGCGCC 1 cut(s) 1685
MlyI GAGTC 3 cut(s) 1060, 1158, 1620
MmeI TCCRAC 1 cut(s) 1158
Mph1103I ATGCAT 1 cut(s) 1759
MseI TTAA 9 cut(s) 92, 257, 366, 372, 467, 1122, 1403, 1769, 1875
MslI CAYNNNNRTG 5 cut(s) 132, 771, 912, 1559, 1806
MspA1I CMGCKG 1 cut(s) 1142
MspI CCGG 5 cut(s) 18, 191, 1322, 2100, 2107
MspR9I CCNGG 7 cut(s) 38, 192, 969, 1283, 2001, 2108, 2276
MunI CAATTG 3 cut(s) 585, 609, 1107
Mva1269I GAATGC 1 cut(s) 1590
MvaI CCWGG 5 cut(s) 38, 969, 1283, 2001, 2276
NarI GGCGCC 1 cut(s) 1685
NciI CCSGG 2 cut(s) 192, 2108
NcoI CCATGG 2 cut(s) 832, 1077
NdeI CATATG 2 cut(s) 245, 535
NdeII GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
NlaIV GGNNCC 4 cut(s) 1368, 1686, 1998, 2082
NmeAIII GCCGAG 1 cut(s) 2059
NmuCI GTSAC 3 cut(s) 1012, 2125, 2282
NsiI ATGCAT 1 cut(s) 1759
NspI RCATGY 3 cut(s) 911, 1805, 1863
NspV TTCGAA 1 cut(s) 2353
PaeR7I CTCGAG 1 cut(s) 1917
PagI TCATGA 1 cut(s) 1629
PaqCI CACCTGC 1 cut(s) 1504
PasI CCCWGGG 1 cut(s) 2000
PceI AGGCCT 1 cut(s) 1521
PciI ACATGT 1 cut(s) 1801
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 1590
PfeI GAWTC 2 cut(s) 1565, 2189
PflFI GACNNNGTC 1 cut(s) 2288
PflMI CCANNNNNTGG 1 cut(s) 1541
PinAI ACCGGT 1 cut(s) 17
PkrI GCNGC 8 cut(s) 765, 880, 979, 1009, 1042, 1096, 1144, 1191
PleI GAGTC 3 cut(s) 1060, 1158, 1620
PluTI GGCGCC 1 cut(s) 1688
PpsI GAGTC 3 cut(s) 1060, 1158, 1620
PpuMI RGGWCCY 1 cut(s) 2081
PscI ACATGT 1 cut(s) 1801
PshBI ATTAAT 1 cut(s) 1122
PsiI TTATAA 1 cut(s) 2312
Psp124BI GAGCTC 1 cut(s) 2058
Psp5II RGGWCCY 1 cut(s) 2081
Psp6I CCWGG 5 cut(s) 36, 967, 1281, 1999, 2274
PspFI CCCAGC 1 cut(s) 2024
PspGI CCWGG 5 cut(s) 36, 967, 1281, 1999, 2274
PspN4I GGNNCC 4 cut(s) 1368, 1686, 1998, 2082
PspPI GGNCC 7 cut(s) 820, 1081, 1319, 1367, 1996, 2081, 2290
PspPPI RGGWCCY 1 cut(s) 2081
PsuI RGATCY 2 cut(s) 1297, 1657
PsyI GACNNNGTC 1 cut(s) 2288
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 6 cut(s) 77, 349, 781, 1104, 1735, 1800
RsaNI GTAC 6 cut(s) 76, 348, 780, 1103, 1734, 1799
RseI CAYNNNNRTG 5 cut(s) 132, 771, 912, 1559, 1806
SacI GAGCTC 1 cut(s) 2058
SalI GTCGAC 1 cut(s) 1477
SaqAI TTAA 9 cut(s) 92, 257, 366, 372, 467, 1122, 1403, 1769, 1875
SatI GCNGC 8 cut(s) 764, 879, 978, 1008, 1041, 1095, 1143, 1190
Sau3AI GATC 5 cut(s) 1297, 1657, 1950, 2180, 2253
Sau96I GGNCC 7 cut(s) 820, 1081, 1319, 1367, 1996, 2081, 2290
SchI GAGTC 3 cut(s) 1060, 1158, 1620
ScrFI CCNGG 7 cut(s) 38, 192, 969, 1283, 2001, 2108, 2276
SduI GDGCHC 3 cut(s) 360, 1433, 2058
SfoI GGCGCC 1 cut(s) 1686
Sfr274I CTCGAG 1 cut(s) 1917
SfuI TTCGAA 1 cut(s) 2353
SinI GGWCC 4 cut(s) 1319, 1367, 2081, 2290
SlaI CTCGAG 1 cut(s) 1917
SmiI ATTTAAAT 1 cut(s) 367
SmiMI CAYNNNNRTG 5 cut(s) 132, 771, 912, 1559, 1806
SmlI CTYRAG 3 cut(s) 1326, 1917, 2195
SmoI CTYRAG 3 cut(s) 1326, 1917, 2195
SseBI AGGCCT 1 cut(s) 1521
SsiI CCGC 3 cut(s) 1486, 1693, 2200
SspDI GGCGCC 1 cut(s) 1684
SspI AATATT 1 cut(s) 402
SspMI CTAG 3 cut(s) 999, 1193, 1581
SstI GAGCTC 1 cut(s) 2058
StuI AGGCCT 1 cut(s) 1521
StyD4I CCNGG 7 cut(s) 36, 190, 967, 1281, 1999, 2106, 2274
StyI CCWWGG 5 cut(s) 564, 832, 1077, 1535, 2016
SwaI ATTTAAAT 1 cut(s) 367
TaaI ACNGT 6 cut(s) 75, 639, 814, 1738, 1946, 2224
TaiI ACGT 1 cut(s) 865
TaqI TCGA 5 cut(s) 714, 1478, 1614, 1918, 2353
TatI WGTACW 1 cut(s) 347
TfiI GAWTC 2 cut(s) 1565, 2189
Tru1I TTAA 9 cut(s) 92, 257, 366, 372, 467, 1122, 1403, 1769, 1875
Tru9I TTAA 9 cut(s) 92, 257, 366, 372, 467, 1122, 1403, 1769, 1875
TscAI CASTG 3 cut(s) 300, 637, 2300
TseFI GTSAC 3 cut(s) 1012, 2125, 2282
TseI GCWGC 8 cut(s) 763, 878, 977, 1007, 1040, 1094, 1142, 1189
Tsp45I GTSAC 3 cut(s) 1012, 2125, 2282
TspDTI ATGAA 7 cut(s) 116, 630, 789, 1253, 1646, 2317, 2322
TspRI CASTG 3 cut(s) 300, 637, 2300
Tth111I GACNNNGTC 1 cut(s) 2288
Van91I CCANNNNNTGG 1 cut(s) 1541
VpaK11BI GGWCC 4 cut(s) 1319, 1367, 2081, 2290
VspI ATTAAT 1 cut(s) 1122
XapI RAATTY 3 cut(s) 368, 786, 2324
XceI RCATGY 3 cut(s) 911, 1805, 1863
XcmI CCANNNNNNNNNTGG 1 cut(s) 830
XhoI CTCGAG 1 cut(s) 1917
XmiI GTMKAC 1 cut(s) 1478
XspI CTAG 3 cut(s) 999, 1193, 1581
Zsp2I ATGCAT 1 cut(s) 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.