MD14G1119100.v1.1

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002846
Physical Location & Seq
Reverse (-)
19338584 .. 19343844
5261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1119100.v1.1.491

Sequence Viewer

Length: 2223 bp
ATGGCGTGGAGAGAGCTTGGCAAAAGGGGGTTACGTGCCGGTCTAACTACGGATTTAACTGCAAGACTACGAAGACATGCAGTGGGATTTACATCTGTGTCTACTGTTGAGGATGTAATAAAATCAGTGTCTGCTGCTGGCAGATGCGTACCAGATCCCTCATTTAAATTTATGTCAACGAAGCTTGATTGTTGTGAAGGTTTGACAAAAGATTCCCTGATACGATGCTATCATGCAAGCCCAGAACTGTTTGCTAGAAAAGGAAATGACGACTCATTTGGTTTAAAAACTCCCAAGAAAGGGAAGTTCAAAAAGAGGGACCATAAGGCCCACCCTCCTGTTGAAGCTCCATATGTGCCTCCTAAACCACAGAGAACTGCCAAATCTATGCCAGATAAGATGATTGAGATTTTTGAAGGCATGACAGTTGTTGAACTTGCGAAGCGTACTGGTGAGTCAATACCGACTCTACAAAGTATTCTTACAAATGTTGGGGAAAAGGTTGACACAGAGTTTGATCCTCTCAGCATCGACGTTTCGGAGCTGATTGCAATGGAAGTTGGGATCAACGTTAGGAGGCTGCACTCCAATGAGGGTACAGAAATTGTACCTCGGCCACCAGTTGTAACTGTCATGGGTCACGTTGACCATGGGAAAACCTCGCTTCTAGATGCTCTACGTCAAACATCTGTGGCTGCCAAGGAAGCTGGAGGAATTACTCAACATCTAGGTGCTTTCGTTGTTGGCATGACATCAGGAGCATCAATTACATTTCTTGACACCCCTGGTCATGCGGCATTTAGTGCGATGCGGGCAAGAGGGGCTGCTGTCACAGATATAGTTGTGCTAGTTGTAGCTGCTGATGATGGGGTGATGCCTCAAACTCTTGAAGCCATGGCTCATGCAAAGGCAGCTAATGTACCGATTGTGGTTGCAATTAATAAATGTGATAAACCGGCTGCTAACCCGGAAAGAATAAAACTCCAGCTTGCTTCTGAGGGTTTGCTGCTGGAGGAGATGGGTGGAGATGTGCAGGTTGTGGAGGTTTCAGCCATAAAGAAGACTGGATTAGATAACTTGGAGGAGGCTTTGCTTCTCCAAGCTGAGATTATGGATCTCAAATCTCGAGTTGATGGGCCTGCTCAAGCTTATGTGGTGGAGGCAAGGCTTGATAAGGGAAAGGGTCCAGCGGTTACAGCAATAGTGAAAGCAGGGACTTTAGAATCCGGGCAGTATGTGGTTGTGGGATCAGAATGGGGCAGAATAAGGGCTATTAGGGACACGGTGGGGAAGTTGGCAGAGAAGGCCAGACCCGCAATGCCAGTTGAGATTGAAGGTTTGAAAGGACTTCCAAGGGCGGGAGATGATATCATTGTTGTGGAATCCGAGGAGCGAGCTAGAATGCTTAGTGCAGGGAGGAAAAAGAAATTTGAGAGAGATAGACTTAGGAAGATTAAAGCTGAGATGGATGTGAGGAATGAGGAAGAAATGTTAAAAGCAAAAAAAGCAGAAGAGTCTGGAGACGAGCCAAAAAATGAGGAACCCAAGCGGGTGGAGTTGCCAATAATAGTAAAAGCAGATGTGCAGGGCACCGTCCAAGCTGTTACAGATGCATTAGCAAATTTAAATAGTCCTCAGGTCTTTGTGAATGTAGTGCATGTTGGTGTTGGGCCTTTGTCTCAGTCTGATTTGGAGATAGCACAGGCCTGTGGTGCATGCATTATTGGATTTAATATAAAGACTCCACCCACTTCCCTCAGTTTGGCTGCAACTCGAGCCAATATAAAGATAATGCTGCATCGTGTTATATATCACCTTCTGGAGGACATTGGCAATTTTATAGTAGACAAAGCTCCTGGAACTTGTGAAACCAAGGTGGCTGGGGAAGCTGAGGTGCTGAGTATTTTTGAGCTCAAAGGAAGGAGCAAGACCAAGGGACCTGATGTGAAGATTGCAGGATGCCGAGTTATTGATGGTTTAATGAGCAAATCGGGAATCTTGAGGCTTCTTAGGAGTGGGGAAGTCGTATTTGAAGGATCCTGTGAATCACTCAAGCGGGAGAAACAGGATGTGGACACGGTAAAGAAGGGGAATGACTGTGGACTGGTAATCCAAGATTACGATGATTTCTGTGTTGGAGACATGGTCCAATGCTTGGAGCAAGTTGTAAGGAAGCCCAGATTCATTTCGTCAGAGAGTGGTGCTGCTCGAATAGAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU IF-2

Protein Analysis

741

Amino Acids

79.9

Weight (kDa)

7.18

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 209 - 365 8.1e-34 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 209 - 315 2.4e-09 50S ribosome-binding GTPase
FeoB_N PF02421 209 - 363 6.1e-06 Ferrous iron transport protein B
EF-G_D2 PF22042 380 - 459 9.3e-20 Elongation factor G domain 2
IF-2 PF11987 500 - 610 7e-28 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1024
AccB1I GGYRCC 1 cut(s) 1589
AccB7I CCANNNNNTGG 1 cut(s) 2155
AccI GTMKAC 2 cut(s) 101, 1845
AciI CCGC 7 cut(s) 794, 811, 1190, 1314, 1358, 1549, 2056
AclI AACGTT 1 cut(s) 570
AclWI GGATC 7 cut(s) 149, 512, 572, 1122, 1255, 2031, 2044
AcoI YGGCCR 1 cut(s) 614
AcsI RAATTY 3 cut(s) 167, 1427, 1621
AfaI GTAC 5 cut(s) 150, 448, 598, 609, 921
AfiI CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1762, 1822, 2155
AgsI TTSAA 8 cut(s) 310, 344, 416, 434, 890, 1334, 1342, 2033
AjnI CCWGG 2 cut(s) 784, 1855
Alw21I GWGCWC 1 cut(s) 1914
Alw26I GTCTC 3 cut(s) 1515, 1683, 2133
AlwI GGATC 7 cut(s) 149, 512, 572, 1122, 1255, 2031, 2044
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 2 cut(s) 1125, 1773
AoxI GGCC 6 cut(s) 327, 614, 1136, 1305, 1670, 1704
ApoI RAATTY 3 cut(s) 167, 1427, 1621
ArsI GACNNNNNNTTYG 4 cut(s) 260, 292, 497, 529
AseI ATTAAT 1 cut(s) 939
AspS9I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1670, 1937, 2146
AsuC2I CCSGG 2 cut(s) 968, 1228
AsuHPI GGTGA 3 cut(s) 464, 883, 1805
AvaI CYCGRG 2 cut(s) 1125, 1773
AvaII GGWCC 4 cut(s) 319, 1184, 1937, 2146
AxyI CCTNAGG 1 cut(s) 1635
BaeGI GKGCMC 1 cut(s) 1592
BamHI GGATCC 1 cut(s) 2036
BanI GGYRCC 1 cut(s) 1589
BanII GRGCYC 1 cut(s) 1914
BbsI GAAGAC 2 cut(s) 79, 1067
Bbv12I GWGCWC 1 cut(s) 1914
BbvCI CCTCAGC 1 cut(s) 1890
BccI CCATC 5 cut(s) 860, 1012, 1127, 1459, 1967
BcgI CGANNNNNNTGC 1 cut(s) 2199
BciT130I CCWGG 2 cut(s) 786, 1857
BcnI CCSGG 2 cut(s) 968, 1228
BcoDI GTCTC 3 cut(s) 1515, 1683, 2133
BfaI CTAG 5 cut(s) 255, 668, 728, 848, 1398
BfuAI ACCTGC 1 cut(s) 1024
Bme1390I CCNGG 4 cut(s) 786, 968, 1228, 1857
Bme18I GGWCC 4 cut(s) 319, 1184, 1937, 2146
BmeT110I CYCGRG 2 cut(s) 1125, 1773
BmgT120I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1670, 1937, 2146
BmiI GGNNCC 6 cut(s) 320, 1185, 1542, 1591, 1938, 2038
BmrFI CCNGG 4 cut(s) 786, 968, 1228, 1857
BpiI GAAGAC 2 cut(s) 79, 1067
BpmI CTGGAG 5 cut(s) 729, 968, 1031, 1539, 1841
Bpu10I CCTNAGC 1 cut(s) 1890
BpuEI CTTGAG 3 cut(s) 1128, 2020, 2036
BpuMI CCSGG 2 cut(s) 968, 1228
BsaAI YACGTR 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 91
BsaJI CCNNGG 9 cut(s) 611, 649, 699, 784, 894, 1352, 1386, 1872, 1932
BsaXI ACNNNNNCTCC 2 cut(s) 2130, 2160
Bsc4I CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1762, 1822, 2155
Bse118I RCCGGY 2 cut(s) 38, 955
Bse1I ACTGG 5 cut(s) 454, 620, 1069, 1322, 2109
Bse21I CCTNAGG 1 cut(s) 1635
Bse3DI GCAATG 2 cut(s) 558, 1323
Bse8I GATNNNNATC 1 cut(s) 91
BseBI CCWGG 2 cut(s) 786, 1857
BseDI CCNNGG 9 cut(s) 611, 649, 699, 784, 894, 1352, 1386, 1872, 1932
BseGI GGATG 4 cut(s) 118, 1474, 1964, 2074
BseJI GATNNNNATC 1 cut(s) 91
BseLI CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1762, 1822, 2155
BseMI GCAATG 2 cut(s) 558, 1323
BseMII CTCAG 9 cut(s) 538, 987, 1095, 1452, 1649, 1694, 1771, 1881, 1889
BseNI ACTGG 5 cut(s) 454, 620, 1069, 1322, 2109
BseRI GAGGAG 3 cut(s) 1028, 1097, 1403
BseSI GKGCMC 1 cut(s) 1592
BseYI CCCAGC 1 cut(s) 1880
BsgI GTGCAG 4 cut(s) 566, 1052, 1431, 1604
BshFI GGCC 6 cut(s) 329, 616, 1138, 1307, 1672, 1706
BshNI GGYRCC 1 cut(s) 1589
BsiHKAI GWGCWC 1 cut(s) 1914
BsiHKCI CYCGRG 2 cut(s) 1125, 1773
BsiSI CCGG 4 cut(s) 39, 956, 968, 1227
BslFI GGGAC 4 cut(s) 332, 1228, 1292, 1950
BslI CCNNNNNNNGG 6 cut(s) 299, 300, 1358, 1762, 1822, 2155
BsmAI GTCTC 3 cut(s) 1515, 1683, 2133
BsmBI CGTCTC 1 cut(s) 1515
BsmFI GGGAC 4 cut(s) 332, 1228, 1292, 1950
BsmI GAATGC 1 cut(s) 1407
BsnI GGCC 6 cut(s) 329, 616, 1138, 1307, 1672, 1706
BsoBI CYCGRG 2 cut(s) 1125, 1773
Bsp1286I GDGCHC 2 cut(s) 1592, 1914
Bsp143I GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
Bsp19I CCATGG 2 cut(s) 649, 894
BspACI CCGC 7 cut(s) 794, 811, 1190, 1314, 1358, 1549, 2056
BspANI GGCC 6 cut(s) 329, 616, 1138, 1307, 1672, 1706
BspCNI CTCAG 9 cut(s) 537, 988, 1096, 1453, 1648, 1693, 1770, 1882, 1890
BspLI GGNNCC 6 cut(s) 320, 1185, 1542, 1591, 1938, 2038
BspMI ACCTGC 1 cut(s) 1024
BspPI GGATC 7 cut(s) 149, 512, 572, 1122, 1255, 2031, 2044
BspT107I GGYRCC 1 cut(s) 1589
BsrDI GCAATG 2 cut(s) 558, 1323
BsrFI RCCGGY 2 cut(s) 38, 955
BsrI ACTGG 5 cut(s) 454, 620, 1069, 1322, 2109
BssAI RCCGGY 2 cut(s) 38, 955
BssECI CCNNGG 9 cut(s) 611, 649, 699, 784, 894, 1352, 1386, 1872, 1932
BssMI GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
BssT1I CCWWGG 6 cut(s) 649, 699, 894, 1352, 1872, 1932
Bst2UI CCWGG 2 cut(s) 786, 1857
Bst4CI ACNGT 8 cut(s) 106, 249, 427, 631, 1285, 1594, 2080, 2099
Bst6I CTCTTC 1 cut(s) 1506
BstAPI GCANNNNNTGC 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 7 cut(s) 139, 238, 813, 990, 1140, 1395, 1717
BstDSI CCRYGG 2 cut(s) 649, 894
BstENI CCTNNNNNAGG 1 cut(s) 1820
BstF5I GGATG 4 cut(s) 118, 1474, 1964, 2074
BstKTI GATC 6 cut(s) 157, 520, 567, 1117, 1250, 2039
BstMAI GTCTC 3 cut(s) 1515, 1683, 2133
BstMBI GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
BstNI CCWGG 2 cut(s) 786, 1857
BstNSI RCATGY 3 cut(s) 80, 1661, 1719
BstSCI CCNGG 4 cut(s) 784, 966, 1226, 1855
BstSLI GKGCMC 1 cut(s) 1592
BstV2I GAAGAC 2 cut(s) 79, 1067
BstX2I RGATCY 3 cut(s) 154, 1114, 2036
BstXI CCANNNNNNTGG 1 cut(s) 1552
BstYI RGATCY 3 cut(s) 154, 1114, 2036
Bsu36I CCTNAGG 1 cut(s) 1635
BsuRI GGCC 6 cut(s) 329, 616, 1138, 1307, 1672, 1706
BtgI CCRYGG 2 cut(s) 649, 894
BtgZI GCGATG 1 cut(s) 821
BtsCI GGATG 4 cut(s) 118, 1474, 1964, 2074
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 132
BveI ACCTGC 1 cut(s) 1024
Cac8I GCNNGC 7 cut(s) 139, 238, 813, 990, 1140, 1395, 1717
CaiI CAGNNNCTG 1 cut(s) 131
Cfr10I RCCGGY 2 cut(s) 38, 955
Cfr13I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1670, 1937, 2146
Csp6I GTAC 5 cut(s) 149, 447, 597, 608, 920
CviQI GTAC 5 cut(s) 149, 447, 597, 608, 920
DpnI GATC 6 cut(s) 156, 519, 566, 1116, 1249, 2038
DpnII GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
DraI TTTAAA 3 cut(s) 166, 285, 1626
EaeI YGGCCR 1 cut(s) 614
Eam1104I CTCTTC 1 cut(s) 1506
EarI CTCTTC 1 cut(s) 1506
Ecl136II GAGCTC 1 cut(s) 1912
Eco130I CCWWGG 6 cut(s) 649, 699, 894, 1352, 1872, 1932
Eco147I AGGCCT 1 cut(s) 1706
Eco24I GRGCYC 1 cut(s) 1914
Eco32I GATATC 1 cut(s) 1369
Eco47I GGWCC 4 cut(s) 319, 1184, 1937, 2146
Eco53kI GAGCTC 1 cut(s) 1912
Eco81I CCTNAGG 1 cut(s) 1635
Eco88I CYCGRG 2 cut(s) 1125, 1773
EcoICRI GAGCTC 1 cut(s) 1912
EcoNI CCTNNNNNAGG 1 cut(s) 1820
EcoO109I RGGNCCY 1 cut(s) 1937
EcoRII CCWGG 2 cut(s) 784, 1855
EcoRV GATATC 1 cut(s) 1369
EcoT14I CCWWGG 6 cut(s) 649, 699, 894, 1352, 1872, 1932
EcoT22I ATGCAT 2 cut(s) 1615, 1721
EcoT38I GRGCYC 1 cut(s) 1914
ErhI CCWWGG 6 cut(s) 649, 699, 894, 1352, 1872, 1932
Esp3I CGTCTC 1 cut(s) 1515
FaqI GGGAC 4 cut(s) 332, 1228, 1292, 1950
FauI CCCGC 5 cut(s) 804, 1321, 1351, 1542, 2049
FauNDI CATATG 1 cut(s) 352
FblI GTMKAC 2 cut(s) 101, 1845
FokI GGATG 4 cut(s) 125, 1481, 1971, 2081
FriOI GRGCYC 1 cut(s) 1914
FspBI CTAG 5 cut(s) 255, 668, 728, 848, 1398
GsaI CCCAGC 1 cut(s) 1884
GsuI CTGGAG 5 cut(s) 729, 968, 1031, 1539, 1841
HaeIII GGCC 6 cut(s) 329, 616, 1138, 1307, 1672, 1706
HapII CCGG 4 cut(s) 39, 956, 968, 1227
HincII GTYRAC 3 cut(s) 177, 505, 646
HindII GTYRAC 3 cut(s) 177, 505, 646
HindIII AAGCTT 2 cut(s) 182, 1146
HpaII CCGG 4 cut(s) 39, 956, 968, 1227
HphI GGTGA 3 cut(s) 464, 883, 1805
Hpy166II GTNNAC 7 cut(s) 102, 177, 505, 646, 1846, 2074, 2102
Hpy188I TCNGA 6 cut(s) 541, 997, 1252, 1387, 1687, 2194
Hpy188III TCNNGA 9 cut(s) 668, 756, 776, 887, 1125, 1518, 1820, 1992, 1999
Hpy8I GTNNAC 7 cut(s) 102, 177, 505, 646, 1846, 2074, 2102
Hpy99I CGWCG 1 cut(s) 536
HpyAV CCTTC 8 cut(s) 191, 410, 1297, 1328, 1826, 1914, 2027, 2080
HpyCH4III ACNGT 8 cut(s) 106, 249, 427, 631, 1285, 1594, 2080, 2099
HpyCH4IV ACGT 5 cut(s) 34, 534, 570, 642, 679
HpySE526I ACGT 5 cut(s) 34, 534, 570, 642, 679
Kzo9I GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
LmnI GCTCC 7 cut(s) 352, 541, 758, 1390, 1858, 1923, 2158
MaeI CTAG 5 cut(s) 255, 668, 728, 848, 1398
MaeII ACGT 5 cut(s) 34, 534, 570, 642, 679
MaeIII GTNAC 6 cut(s) 30, 625, 638, 829, 1192, 1603
MalI GATC 6 cut(s) 156, 519, 566, 1116, 1249, 2038
MboI GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
MboII GAAGA 6 cut(s) 84, 1072, 1462, 1496, 1523, 1960
MflI RGATCY 3 cut(s) 154, 1114, 2036
MhlI GDGCHC 2 cut(s) 1592, 1914
MluCI AATT 8 cut(s) 167, 603, 714, 765, 936, 1427, 1621, 1834
MlyI GAGTC 5 cut(s) 266, 460, 464, 1523, 1735
MmeI TCCRAC 1 cut(s) 2116
Mph1103I ATGCAT 2 cut(s) 1615, 1721
MseI TTAA 9 cut(s) 56, 165, 284, 939, 1455, 1493, 1625, 1731, 1979
MslI CAYNNNNRTG 4 cut(s) 588, 729, 1376, 1662
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 4 cut(s) 39, 956, 968, 1227
MspR9I CCNGG 4 cut(s) 786, 968, 1228, 1857
Mva1269I GAATGC 1 cut(s) 1407
MvaI CCWGG 2 cut(s) 786, 1857
NciI CCSGG 2 cut(s) 968, 1228
NcoI CCATGG 2 cut(s) 649, 894
NdeI CATATG 1 cut(s) 352
NdeII GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
NlaIV GGNNCC 6 cut(s) 320, 1185, 1542, 1591, 1938, 2038
NmeAIII GCCGAG 2 cut(s) 592, 1988
NmuCI GTSAC 2 cut(s) 638, 829
NsiI ATGCAT 2 cut(s) 1615, 1721
NspI RCATGY 3 cut(s) 80, 1661, 1719
PaeI GCATGC 1 cut(s) 1719
PaeR7I CTCGAG 2 cut(s) 1125, 1773
PceI AGGCCT 1 cut(s) 1706
PctI GAATGC 1 cut(s) 1407
PfeI GAWTC 6 cut(s) 212, 1223, 1382, 1995, 2045, 2181
PflFI GACNNNGTC 1 cut(s) 2144
PflMI CCANNNNNTGG 1 cut(s) 2155
PfoI TCCNGGA 1 cut(s) 1855
PleI GAGTC 5 cut(s) 266, 460, 463, 1522, 1735
PpsI GAGTC 5 cut(s) 266, 460, 463, 1522, 1735
Ppu21I YACGTR 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 1937
PshBI ATTAAT 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 1914
Psp1406I AACGTT 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 1937
Psp6I CCWGG 2 cut(s) 784, 1855
PspFI CCCAGC 1 cut(s) 1880
PspGI CCWGG 2 cut(s) 784, 1855
PspN4I GGNNCC 6 cut(s) 320, 1185, 1542, 1591, 1938, 2038
PspPI GGNCC 7 cut(s) 319, 328, 1136, 1184, 1670, 1937, 2146
PspPPI RGGWCCY 1 cut(s) 1937
PspXI VCTCGAGB 1 cut(s) 1773
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 3 cut(s) 154, 1114, 2036
PsyI GACNNNGTC 1 cut(s) 2144
RsaI GTAC 5 cut(s) 150, 448, 598, 609, 921
RsaNI GTAC 5 cut(s) 149, 447, 597, 608, 920
RseI CAYNNNNRTG 4 cut(s) 588, 729, 1376, 1662
SacI GAGCTC 1 cut(s) 1914
SaqAI TTAA 9 cut(s) 56, 165, 284, 939, 1455, 1493, 1625, 1731, 1979
Sau3AI GATC 6 cut(s) 154, 517, 564, 1114, 1247, 2036
Sau96I GGNCC 7 cut(s) 319, 328, 1136, 1184, 1670, 1937, 2146
SchI GAGTC 5 cut(s) 266, 460, 464, 1523, 1735
ScrFI CCNGG 4 cut(s) 786, 968, 1228, 1857
SduI GDGCHC 2 cut(s) 1592, 1914
Sfr274I CTCGAG 2 cut(s) 1125, 1773
SinI GGWCC 4 cut(s) 319, 1184, 1937, 2146
SlaI CTCGAG 2 cut(s) 1125, 1773
SmiI ATTTAAAT 2 cut(s) 166, 1626
SmiMI CAYNNNNRTG 4 cut(s) 588, 729, 1376, 1662
SmlI CTYRAG 5 cut(s) 1125, 1143, 1773, 1999, 2051
SmoI CTYRAG 5 cut(s) 1125, 1143, 1773, 1999, 2051
SphI GCATGC 1 cut(s) 1719
Sse9I AATT 8 cut(s) 167, 603, 714, 765, 936, 1427, 1621, 1834
SseBI AGGCCT 1 cut(s) 1706
SsiI CCGC 7 cut(s) 794, 811, 1190, 1314, 1358, 1549, 2056
SspMI CTAG 5 cut(s) 255, 668, 728, 848, 1398
SstI GAGCTC 1 cut(s) 1914
StuI AGGCCT 1 cut(s) 1706
StyD4I CCNGG 4 cut(s) 784, 966, 1226, 1855
StyI CCWWGG 6 cut(s) 649, 699, 894, 1352, 1872, 1932
SwaI ATTTAAAT 2 cut(s) 166, 1626
TaaI ACNGT 8 cut(s) 106, 249, 427, 631, 1285, 1594, 2080, 2099
TaiI ACGT 5 cut(s) 37, 537, 573, 645, 682
TaqI TCGA 4 cut(s) 531, 1126, 1774, 2209
TasI AATT 8 cut(s) 167, 603, 714, 765, 936, 1427, 1621, 1834
TauI GCSGC 1 cut(s) 797
TfiI GAWTC 6 cut(s) 212, 1223, 1382, 1995, 2045, 2181
Tru1I TTAA 9 cut(s) 56, 165, 284, 939, 1455, 1493, 1625, 1731, 1979
Tru9I TTAA 9 cut(s) 56, 165, 284, 939, 1455, 1493, 1625, 1731, 1979
TscAI CASTG 2 cut(s) 87, 132
TseFI GTSAC 2 cut(s) 638, 829
Tsp45I GTSAC 2 cut(s) 638, 829
TspDTI ATGAA 1 cut(s) 2173
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 2 cut(s) 87, 132
Tth111I GACNNNGTC 1 cut(s) 2144
Van91I CCANNNNNTGG 1 cut(s) 2155
VpaK11BI GGWCC 4 cut(s) 319, 1184, 1937, 2146
VspI ATTAAT 1 cut(s) 939
XagI CCTNNNNNAGG 1 cut(s) 1820
XapI RAATTY 3 cut(s) 167, 1427, 1621
XbaI TCTAGA 1 cut(s) 667
XceI RCATGY 3 cut(s) 80, 1661, 1719
XhoI CTCGAG 2 cut(s) 1125, 1773
XmiI GTMKAC 2 cut(s) 101, 1845
XspI CTAG 5 cut(s) 255, 668, 728, 848, 1398
Zsp2I ATGCAT 2 cut(s) 1615, 1721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.