Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012359

Physical Location & Seq

Genomic Coordinates Forward (+)

26645912 .. 26650494

Gene Structure 4583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1124400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGAATTTTGGAGCAGCAGCCGCAAGAGCTAGAGATGCTGGGTTTTCTACTGAGGATGACCAAAATATGGTGAAAGGTAAGCTTCCTTTCTCATATTCTTCCTCTCCCTCTTCATCTTCATCTTCTCCTCACTACAACAAAACCACCGGCGTAGCCACTCTGGTCCCTTCTTCCGCTGTCAGCAGAAGCCGCAGCTGGCACCACGGCAGCGGCAGCCCCGACACGAAGCTCGAGCTTATGGACTCGACCCCCCAAGAAAACAACGAAGATGGTGGTGCTCAAAGAGAAGAAAACCCAATCCCCATCGAGAGGGAGCACATGTTCGACAAAGTCGTGACGCCGAGCGACGTAGGGAAGCTCAACCGCCTCGTCATCCCGAAACAGCATGCCGAGAAGTACTTCCCGTTGGATTCTTCCAGCAACGAGAAAGGGCTTCTGCTGAATTTCGAGGACCGGAACGGGAAGCCGTGGCGGTTTAGGTACTCCTACTGGAACAGCAGCCAGAGCTACGTGATGACCAAAGGGTGGAGCAGGTTTGTGAAGGAGAAAAAGCTCGACGCCGGCGACATAGTCTCGTTCCAGCGAGGACTTGGTGAGGTGGGGAAAGACCGTCTTTTTATTGATTGGCGGCGCCGCCCAGATGCGCCTGATCCAAACCATCACCACCACCACCACCCGCTTCATCCACATTATCAGCCCAATTTTGCAATTCCTCATCAGCATCAATACTCCTGGAACCCTCACCGGTCGGTTCCATGGAGTCCGTTGCTGATGAGACCTCCCGGGGCAGTAGTGACTCAGGACCACTTGCTCTTGTCGCAGCAGCAGATTAATTACGGCGTCAATATGCATCACCCATATCGAAATACTGGGACAAGTGGTGGTGGTTCAGGTTCTTATGGTTACGGAAATGTGGTGAACTCTACTAGGCCGATTTATTATTTGAGATCAGCTTCATCCGCTGCCCCGCCACAGTTTCACGAGCAAGGGGACAACGGAGTAATAGAATTTCAACAACACCAACAAGTACAGCTGGGTCGGGTTCATGAGTCGAATATGGTTCTGGAGTCGGTGCCAGTGGTCCATGGGAAGGCGGCAGCCAAGAAGCTGAGGCTATTTGGAGTGAACATGGAGTGTCCCATATCGGAGTCGGATCATGAATGTGACATATTGTCTTCAACCACATTATCATCATCCCAGTATCATCATCATCATGCCCATCACCAACTTTCGCCGCCATCGCAACCCCCACCGCTCCAATTAAGGCTCTACGACGGCATGCCTCTCCTCCACCAAACCATGCCCCCTACCACCACTACTAGTATCACCACCGACTTTCTCAACATGAGTGGCAAGGCCTCCCCATCTTCCATGTCCTTGGACTATGATGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009889 GO:0009908 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0099402 GO:0140110 GO:1900618 GO:1901371 GO:1903506 GO:1905421 GO:2000024 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

465

Amino Acids

51.97

Weight (kDa)

7.22

Isoelectric Point (pI)

57.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	108 - 212	1.7e-26	B3 DNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46870
fragaria_vesca	FvH4_5g10410
malus_domestica	MD06G1124400.v1.1 MD14G1141400.v1.1
prunus_persica	Prupe.5G134900_v2.0.a1
pyrus_communis	pycom06g11470 pycom14g11620
rosa_chinensis	RchiOBHm_Chr7g0187821
rosa_laevigata	RLG00000004754
rosa_multiflora	Rmu_co8399923.1_g000001 Rmu_sc0004483.1_g000041
rosa_roxburghii	Rroxscaffold_3G00267040
rosa_rugosa	Rorug06G0484500 Rorug06G0484600
rosa_samantha	Rh7AG089800 Rh7BG091400 Rh7CG090500 Rh7DG092200
rosa_wichuraiana	Rw7G007740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1388
Acc36I	ACCTGC	1 cut(s)	522
AccB1I	GGYRCC	3 cut(s)	198, 630, 1072
AccB7I	CCANNNNNTGG	2 cut(s)	67, 525
AccBSI	CCGCTC	1 cut(s)	1255
AclWI	GGATC	2 cut(s)	644, 1161
AcsI	RAATTY	3 cut(s)	4, 442, 1007
AcyI	GRCGYC	4 cut(s)	338, 558, 631, 840
AfaI	GTAC	3 cut(s)	398, 482, 1029
AfiI	CCNNNNNNNGG	4 cut(s)	67, 309, 525, 1090
AflIII	ACRYGT	1 cut(s)	318
AgeI	ACCGGT	1 cut(s)	744
AgsI	TTSAA	2 cut(s)	1013, 1179
AhdI	GACNNNNNGTC	1 cut(s)	1171
AhlI	ACTAGT	1 cut(s)	1319
AjnI	CCWGG	1 cut(s)	731
AloI	GAACNNNNNNTCC	2 cut(s)	305, 337
Alw21I	GWGCWC	2 cut(s)	280, 318
Alw26I	GTCTC	2 cut(s)	577, 769
AlwI	GGATC	2 cut(s)	644, 1161
Ama87I	CYCGRG	2 cut(s)	230, 782
AoxI	GGCC	2 cut(s)	929, 1356
ApoI	RAATTY	3 cut(s)	4, 442, 1007
AseI	ATTAAT	1 cut(s)	831
AsiGI	ACCGGT	1 cut(s)	744
Asp700I	GAANNNNTTC	1 cut(s)	398
AspLEI	GCGC	2 cut(s)	633, 646
AspS9I	GGNCC	4 cut(s)	163, 451, 802, 1081
AsuC2I	CCSGG	2 cut(s)	783, 784
AsuHPI	GGTGA	8 cut(s)	82, 605, 653, 734, 845, 928, 1214, 1318
AvaI	CYCGRG	2 cut(s)	230, 782
AvaII	GGWCC	4 cut(s)	163, 451, 802, 1081
BanI	GGYRCC	3 cut(s)	198, 630, 1072
BauI	CACGAG	1 cut(s)	980
BbsI	GAAGAC	1 cut(s)	1167
Bbv12I	GWGCWC	2 cut(s)	280, 318
BbvCI	CCTCAGC	1 cut(s)	1109
BccI	CCATC	6 cut(s)	263, 311, 666, 1227, 1246, 1372
BceAI	ACGGC	4 cut(s)	220, 451, 853, 1291
BciT130I	CCWGG	1 cut(s)	733
BcnI	CCSGG	2 cut(s)	783, 784
BcoDI	GTCTC	2 cut(s)	577, 769
BcuI	ACTAGT	1 cut(s)	1319
BfaI	CTAG	4 cut(s)	30, 927, 1320, 1393
BfoI	RGCGCY	1 cut(s)	634
BfuAI	ACCTGC	1 cut(s)	522
BmcAI	AGTACT	1 cut(s)	398
Bme1390I	CCNGG	3 cut(s)	733, 783, 784
Bme18I	GGWCC	4 cut(s)	163, 451, 802, 1081
BmeRI	GACNNNNNGTC	1 cut(s)	1171
BmeT110I	CYCGRG	2 cut(s)	230, 782
BmgT120I	GGNCC	4 cut(s)	163, 451, 802, 1081
BmiI	GGNNCC	6 cut(s)	165, 200, 632, 737, 753, 1074
BmrFI	CCNGG	3 cut(s)	733, 783, 784
BmrI	ACTGGG	2 cut(s)	879, 1192
BmsI	GCATC	4 cut(s)	25, 631, 730, 859
BmuI	ACTGGG	2 cut(s)	879, 1192
BpiI	GAAGAC	1 cut(s)	1167
BpmI	CTGGAG	1 cut(s)	1085
Bpu10I	CCTNAGC	1 cut(s)	1109
BpuMI	CCSGG	2 cut(s)	783, 784
BsaAI	YACGTR	1 cut(s)	511
BsaHI	GRCGYC	4 cut(s)	338, 558, 631, 840
BsaI	GGTCTC	1 cut(s)	769
BsaJI	CCNNGG	7 cut(s)	202, 467, 755, 782, 783, 1084, 1377
BsaWI	WCCGGW	2 cut(s)	453, 744
BsaXI	ACNNNNNCTCC	2 cut(s)	305, 335
Bsc4I	CCNNNNNNNGG	4 cut(s)	67, 309, 525, 1090
Bse118I	RCCGGY	3 cut(s)	146, 560, 744
Bse1I	ACTGG	4 cut(s)	494, 874, 1076, 1198
BseBI	CCWGG	1 cut(s)	733
BseDI	CCNNGG	7 cut(s)	202, 467, 755, 782, 783, 1084, 1377
BseGI	GGATG	5 cut(s)	61, 372, 682, 956, 1193
BseLI	CCNNNNNNNGG	4 cut(s)	67, 309, 525, 1090
BseMII	CTCAG	3 cut(s)	42, 812, 1100
BseNI	ACTGG	4 cut(s)	494, 874, 1076, 1198
BseRI	GAGGAG	2 cut(s)	117, 1277
BseYI	CCCAGC	2 cut(s)	38, 1033
Bsh1285I	CGRYCG	1 cut(s)	749
BshFI	GGCC	2 cut(s)	931, 1358
BshNI	GGYRCC	3 cut(s)	198, 630, 1072
BshTI	ACCGGT	1 cut(s)	744
BsiEI	CGRYCG	1 cut(s)	749
BsiHKAI	GWGCWC	2 cut(s)	280, 318
BsiHKCI	CYCGRG	2 cut(s)	230, 782
BsiSI	CCGG	5 cut(s)	147, 454, 561, 745, 783
BslFI	GGGAC	4 cut(s)	149, 886, 1004, 1122
BslI	CCNNNNNNNGG	4 cut(s)	67, 309, 525, 1090
BsmAI	GTCTC	2 cut(s)	577, 769
BsmFI	GGGAC	4 cut(s)	149, 886, 1004, 1122
BsnI	GGCC	2 cut(s)	931, 1358
Bso31I	GGTCTC	1 cut(s)	769
BsoBI	CYCGRG	2 cut(s)	230, 782
Bsp1286I	GDGCHC	2 cut(s)	280, 318
Bsp143I	GATC	3 cut(s)	649, 947, 1153
Bsp19I	CCATGG	2 cut(s)	755, 1084
BspANI	GGCC	2 cut(s)	931, 1358
BspCNI	CTCAG	3 cut(s)	43, 811, 1101
BspHI	TCATGA	2 cut(s)	1045, 1156
BspLI	GGNNCC	6 cut(s)	165, 200, 632, 737, 753, 1074
BspMI	ACCTGC	1 cut(s)	522
BspPI	GGATC	2 cut(s)	644, 1161
BspT107I	GGYRCC	3 cut(s)	198, 630, 1072
BspTNI	GGTCTC	1 cut(s)	769
BsrBI	CCGCTC	1 cut(s)	1255
BsrFI	RCCGGY	3 cut(s)	146, 560, 744
BsrI	ACTGG	4 cut(s)	494, 874, 1076, 1198
BssAI	RCCGGY	3 cut(s)	146, 560, 744
BssECI	CCNNGG	7 cut(s)	202, 467, 755, 782, 783, 1084, 1377
BssMI	GATC	3 cut(s)	649, 947, 1153
BssNI	GRCGYC	4 cut(s)	338, 558, 631, 840
BssSI	CACGAG	1 cut(s)	980
BssT1I	CCWWGG	3 cut(s)	755, 1084, 1377
Bst2BI	CACGAG	1 cut(s)	980
Bst2UI	CCWGG	1 cut(s)	733
Bst4CI	ACNGT	2 cut(s)	611, 975
Bst6I	CTCTTC	1 cut(s)	115
BstACI	GRCGYC	4 cut(s)	338, 558, 631, 840
BstBAI	YACGTR	1 cut(s)	511
BstC8I	GCNNGC	4 cut(s)	197, 387, 562, 1280
BstDEI	CTNAG	3 cut(s)	51, 798, 1109
BstDSI	CCRYGG	4 cut(s)	202, 467, 755, 1084
BstF5I	GGATG	5 cut(s)	61, 372, 682, 956, 1193
BstH2I	RGCGCY	1 cut(s)	634
BstHHI	GCGC	2 cut(s)	633, 646
BstKTI	GATC	3 cut(s)	652, 950, 1156
BstMAI	GTCTC	2 cut(s)	577, 769
BstMBI	GATC	3 cut(s)	649, 947, 1153
BstMCI	CGRYCG	1 cut(s)	749
BstMWI	GCNNNNNNNGC	9 cut(s)	20, 26, 35, 189, 213, 504, 817, 959, 1240
BstNI	CCWGG	1 cut(s)	733
BstNSI	RCATGY	3 cut(s)	322, 389, 1282
BstSCI	CCNGG	3 cut(s)	731, 781, 782
BstV2I	GAAGAC	1 cut(s)	1167
BstXI	CCANNNNNNTGG	1 cut(s)	1378
BsuRI	GGCC	2 cut(s)	931, 1358
BtgI	CCRYGG	4 cut(s)	202, 467, 755, 1084
BtgZI	GCGATG	1 cut(s)	1224
BtsCI	GGATG	5 cut(s)	61, 372, 682, 956, 1193
BtsIMutI	CAGTG	1 cut(s)	1083
BveI	ACCTGC	1 cut(s)	522
Cac8I	GCNNGC	4 cut(s)	197, 387, 562, 1280
CciI	TCATGA	2 cut(s)	1045, 1156
CfoI	GCGC	2 cut(s)	633, 646
Cfr10I	RCCGGY	3 cut(s)	146, 560, 744
Cfr13I	GGNCC	4 cut(s)	163, 451, 802, 1081
Cfr9I	CCCGGG	1 cut(s)	782
CseI	GACGC	3 cut(s)	346, 566, 829
Csp6I	GTAC	3 cut(s)	397, 481, 1028
CspAI	ACCGGT	1 cut(s)	744
CspCI	CAANNNNNGTGG	2 cut(s)	1330, 1365
CviQI	GTAC	3 cut(s)	397, 481, 1028
DdeI	CTNAG	3 cut(s)	51, 798, 1109
DinI	GGCGCC	1 cut(s)	632
DpnI	GATC	3 cut(s)	651, 949, 1155
DpnII	GATC	3 cut(s)	649, 947, 1153
DrdI	GACNNNNNNGTC	1 cut(s)	1388
DriI	GACNNNNNGTC	1 cut(s)	1171
DseDI	GACNNNNNNGTC	1 cut(s)	1388
Eam1104I	CTCTTC	1 cut(s)	115
Eam1105I	GACNNNNNGTC	1 cut(s)	1171
EarI	CTCTTC	1 cut(s)	115
Eco130I	CCWWGG	3 cut(s)	755, 1084, 1377
Eco147I	AGGCCT	1 cut(s)	1358
Eco31I	GGTCTC	1 cut(s)	769
Eco47I	GGWCC	4 cut(s)	163, 451, 802, 1081
Eco88I	CYCGRG	2 cut(s)	230, 782
EcoRII	CCWGG	1 cut(s)	731
EcoT14I	CCWWGG	3 cut(s)	755, 1084, 1377
EcoT22I	ATGCAT	1 cut(s)	852
EgeI	GGCGCC	1 cut(s)	632
EheI	GGCGCC	1 cut(s)	632
ErhI	CCWWGG	3 cut(s)	755, 1084, 1377
FalI	AAGNNNNNCTT	4 cut(s)	66, 98, 597, 629
FaqI	GGGAC	4 cut(s)	149, 886, 1004, 1122
FauI	CCCGC	2 cut(s)	684, 975
FokI	GGATG	5 cut(s)	68, 359, 669, 943, 1180
FspBI	CTAG	4 cut(s)	30, 927, 1320, 1393
GlaI	GCGC	2 cut(s)	632, 645
GsaI	CCCAGC	2 cut(s)	42, 1037
GsuI	CTGGAG	1 cut(s)	1085
HaeII	RGCGCY	1 cut(s)	634
HaeIII	GGCC	2 cut(s)	931, 1358
HapII	CCGG	5 cut(s)	147, 454, 561, 745, 783
HgaI	GACGC	3 cut(s)	346, 566, 829
HhaI	GCGC	2 cut(s)	633, 646
Hin1I	GRCGYC	4 cut(s)	338, 558, 631, 840
Hin6I	GCGC	2 cut(s)	631, 644
HinP1I	GCGC	2 cut(s)	631, 644
HindIII	AAGCTT	1 cut(s)	80
HinfI	GANTC	7 cut(s)	242, 410, 760, 796, 1049, 1067, 1148
HpaII	CCGG	5 cut(s)	147, 454, 561, 745, 783
HphI	GGTGA	8 cut(s)	82, 605, 653, 734, 845, 928, 1214, 1318
Hpy166II	GTNNAC	2 cut(s)	919, 1126
Hpy188I	TCNGA	2 cut(s)	1147, 1153
Hpy188III	TCNNGA	8 cut(s)	307, 334, 376, 800, 980, 1046, 1064, 1157
Hpy8I	GTNNAC	2 cut(s)	919, 1126
Hpy99I	CGWCG	3 cut(s)	350, 560, 1277
HpyAV	CCTTC	3 cut(s)	177, 535, 1084
HpyCH4III	ACNGT	2 cut(s)	611, 975
HpyCH4IV	ACGT	2 cut(s)	348, 510
HpyCH4V	TGCA	2 cut(s)	707, 850
HpyF10VI	GCNNNNNNNGC	9 cut(s)	20, 26, 35, 189, 213, 504, 817, 959, 1240
HpyF3I	CTNAG	3 cut(s)	51, 798, 1109
HpySE526I	ACGT	2 cut(s)	348, 510
Hsp92I	GRCGYC	4 cut(s)	338, 558, 631, 840
HspAI	GCGC	2 cut(s)	631, 644
KasI	GGCGCC	1 cut(s)	630
KroI	GCCGGC	1 cut(s)	560
KroNI	GCCGGC	1 cut(s)	562
Kzo9I	GATC	3 cut(s)	649, 947, 1153
LmnI	GCTCC	4 cut(s)	11, 313, 528, 1260
LweI	GCATC	4 cut(s)	25, 631, 730, 859
MaeI	CTAG	4 cut(s)	30, 927, 1320, 1393
MaeII	ACGT	2 cut(s)	348, 510
MaeIII	GTNAC	4 cut(s)	334, 793, 902, 1163
MalI	GATC	3 cut(s)	651, 949, 1155
MbiI	CCGCTC	1 cut(s)	1255
MboI	GATC	3 cut(s)	649, 947, 1153
MhlI	GDGCHC	2 cut(s)	280, 318
MluCI	AATT	7 cut(s)	4, 442, 700, 708, 832, 1007, 1259
Mly113I	GGCGCC	1 cut(s)	631
MlyI	GAGTC	6 cut(s)	236, 769, 790, 1058, 1076, 1157
MmeI	TCCRAC	2 cut(s)	387, 1131
Mph1103I	ATGCAT	1 cut(s)	852
MreI	CGCCGGCG	1 cut(s)	560
MroNI	GCCGGC	1 cut(s)	560
MroXI	GAANNNNTTC	1 cut(s)	398
MseI	TTAA	2 cut(s)	831, 1262
MslI	CAYNNNNRTG	2 cut(s)	1161, 1212
MspA1I	CMGCKG	5 cut(s)	176, 195, 210, 962, 1033
MspI	CCGG	5 cut(s)	147, 454, 561, 745, 783
MspR9I	CCNGG	3 cut(s)	733, 783, 784
MvaI	CCWGG	1 cut(s)	733
MwoI	GCNNNNNNNGC	9 cut(s)	20, 26, 35, 189, 213, 504, 817, 959, 1240
NaeI	GCCGGC	1 cut(s)	562
NarI	GGCGCC	1 cut(s)	631
NciI	CCSGG	2 cut(s)	783, 784
NcoI	CCATGG	2 cut(s)	755, 1084
NdeII	GATC	3 cut(s)	649, 947, 1153
NgoMIV	GCCGGC	1 cut(s)	560
NlaIV	GGNNCC	6 cut(s)	165, 200, 632, 737, 753, 1074
NmeAIII	GCCGAG	2 cut(s)	366, 415
NmuCI	GTSAC	3 cut(s)	334, 793, 1163
NsiI	ATGCAT	1 cut(s)	852
NspI	RCATGY	3 cut(s)	322, 389, 1282
PaeI	GCATGC	2 cut(s)	389, 1282
PaeR7I	CTCGAG	1 cut(s)	230
PagI	TCATGA	2 cut(s)	1045, 1156
PceI	AGGCCT	1 cut(s)	1358
PciI	ACATGT	1 cut(s)	318
PcsI	WCGNNNNNNNCGW	2 cut(s)	330, 561
PdiI	GCCGGC	1 cut(s)	562
PdmI	GAANNNNTTC	1 cut(s)	398
PfeI	GAWTC	1 cut(s)	410
PflFI	GACNNNGTC	2 cut(s)	329, 569
PflMI	CCANNNNNTGG	2 cut(s)	67, 525
PfoI	TCCNGGA	1 cut(s)	731
PinAI	ACCGGT	1 cut(s)	744
PleI	GAGTC	6 cut(s)	236, 768, 790, 1057, 1075, 1156
PluTI	GGCGCC	1 cut(s)	634
PpsI	GAGTC	6 cut(s)	236, 768, 790, 1057, 1075, 1156
Ppu21I	YACGTR	1 cut(s)	511
PscI	ACATGT	1 cut(s)	318
PshBI	ATTAAT	1 cut(s)	831
Psp6I	CCWGG	1 cut(s)	731
PspFI	CCCAGC	2 cut(s)	38, 1033
PspGI	CCWGG	1 cut(s)	731
PspN4I	GGNNCC	6 cut(s)	165, 200, 632, 737, 753, 1074
PspPI	GGNCC	4 cut(s)	163, 451, 802, 1081
PspXI	VCTCGAGB	1 cut(s)	230
PsyI	GACNNNGTC	2 cut(s)	329, 569
PvuII	CAGCTG	2 cut(s)	195, 1033
RsaI	GTAC	3 cut(s)	398, 482, 1029
RsaNI	GTAC	3 cut(s)	397, 481, 1028
RseI	CAYNNNNRTG	2 cut(s)	1161, 1212
SaqAI	TTAA	2 cut(s)	831, 1262
Sau3AI	GATC	3 cut(s)	649, 947, 1153
Sau96I	GGNCC	4 cut(s)	163, 451, 802, 1081
ScaI	AGTACT	1 cut(s)	398
SchI	GAGTC	6 cut(s)	236, 769, 790, 1058, 1076, 1157
ScrFI	CCNGG	3 cut(s)	733, 783, 784
SduI	GDGCHC	2 cut(s)	280, 318
SfaNI	GCATC	4 cut(s)	25, 631, 730, 859
SfoI	GGCGCC	1 cut(s)	632
Sfr274I	CTCGAG	1 cut(s)	230
SgrAI	CRCCGGYG	2 cut(s)	146, 560
SinI	GGWCC	4 cut(s)	163, 451, 802, 1081
SlaI	CTCGAG	1 cut(s)	230
SmaI	CCCGGG	1 cut(s)	784
SmiMI	CAYNNNNRTG	2 cut(s)	1161, 1212
SmlI	CTYRAG	1 cut(s)	230
SmoI	CTYRAG	1 cut(s)	230
SpeI	ACTAGT	1 cut(s)	1319
SphI	GCATGC	2 cut(s)	389, 1282
Sse9I	AATT	7 cut(s)	4, 442, 700, 708, 832, 1007, 1259
SseBI	AGGCCT	1 cut(s)	1358
SspDI	GGCGCC	1 cut(s)	630
SspMI	CTAG	4 cut(s)	30, 927, 1320, 1393
StuI	AGGCCT	1 cut(s)	1358
StyD4I	CCNGG	3 cut(s)	731, 781, 782
StyI	CCWWGG	3 cut(s)	755, 1084, 1377
TaaI	ACNGT	2 cut(s)	611, 975
TaiI	ACGT	2 cut(s)	351, 513
TaqI	TCGA	8 cut(s)	231, 245, 306, 324, 447, 555, 862, 1052
TasI	AATT	7 cut(s)	4, 442, 700, 708, 832, 1007, 1259
TatI	WGTACW	2 cut(s)	396, 1027
TauI	GCSGC	7 cut(s)	23, 192, 213, 631, 636, 1097, 1237
TfiI	GAWTC	1 cut(s)	410
Tru1I	TTAA	2 cut(s)	831, 1262
Tru9I	TTAA	2 cut(s)	831, 1262
TscAI	CASTG	1 cut(s)	1083
TseFI	GTSAC	3 cut(s)	334, 793, 1163
Tsp45I	GTSAC	3 cut(s)	334, 793, 1163
TspDTI	ATGAA	7 cut(s)	17, 102, 108, 671, 945, 1034, 1173
TspGWI	ACGGA	3 cut(s)	753, 921, 1011
TspMI	CCCGGG	1 cut(s)	782
TspRI	CASTG	1 cut(s)	1083
Tth111I	GACNNNGTC	2 cut(s)	329, 569
Van91I	CCANNNNNTGG	2 cut(s)	67, 525
VpaK11BI	GGWCC	4 cut(s)	163, 451, 802, 1081
VspI	ATTAAT	1 cut(s)	831
XapI	RAATTY	3 cut(s)	4, 442, 1007
XceI	RCATGY	3 cut(s)	322, 389, 1282
XcmI	CCANNNNNNNNNTGG	1 cut(s)	587
XhoI	CTCGAG	1 cut(s)	230
XmaI	CCCGGG	1 cut(s)	782
XmnI	GAANNNNTTC	1 cut(s)	398
XspI	CTAG	4 cut(s)	30, 927, 1320, 1393
ZrmI	AGTACT	1 cut(s)	398
Zsp2I	ATGCAT	1 cut(s)	852

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.