MD06G1128100.v1.1

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015850
Physical Location & Seq
Forward (+)
27046491 .. 27049303
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1128100.v1.1.491

Sequence Viewer

Length: 558 bp
ATGTCGTCGTATACGCTGCATTTGGCTTCGCCGGCGACCAGAAATTGTTTGTACGAACACCATCGCAGAATTCCCACATTCAAATCTCCCGCGTTTTCCAACCCCCATCCCTTTCGCTGCGATTACGCTGCGGGAAATGGGCTATTGGCAGCGAGCAACACAAGCTTTAAAGCCGTGCCCAAAACGCACCATCGTCGGTTCTCGTGCACTTCTCAGCTGGCGGAGATGGCTCCGGCTACCTCTGCAGTCTACGGCTTCCTCCTCCTCAGCGGCGGCTTCTTTGCTTTTTCGAGAACAGGAAGCAAAGGGTCGCTTATCGGTGGTGTTTCAGGAGCAGCTCTAATGGCAACTGCTTACTATCTGCTGCAAACAACAGAGACAAAAGCTATTGGTGATGCCCTTGGTTTTGGATCAGCATTTCTTTTTGCTGCGGTATTCGGTATACGATTAGCAGCTACACGGAAATTGGCTCCTGCAGGCCCTCTGTTAGCTCTTTCTCTTAGTGCACTGGCTGTGTTTATATCAGCTTATCTAAAAGATAGTGTCACCATATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_14

Protein Analysis

186

Amino Acids

19.48

Weight (kDa)

9.91

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 81 - 173 3.3e-16 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33265
fragaria_vesca FvH4_5g10080 FvH4_5g10080
malus_domestica MD06G1128100.v1.1
prunus_persica Prupe.5G137900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0188191
rosa_laevigata RLG00000004720
rosa_multiflora Rmu_sc0004001.1_g000016 Rmu_sc0015196.1_g000001
rosa_roxburghii Rroxscaffold_3G00266680
rosa_rugosa Rorug06G0489000
rosa_samantha Rh7AG093300 Rh7BG094800 Rh7CG094600 Rh7DG095200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 11, 249, 442
AccII CGCG 1 cut(s) 92
AciI CCGC 6 cut(s) 90, 131, 221, 270, 273, 431
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 1 cut(s) 69
AfaI GTAC 1 cut(s) 53
AgsI TTSAA 1 cut(s) 82
AluBI AGCT 7 cut(s) 165, 217, 338, 386, 455, 491, 527
AluI AGCT 7 cut(s) 165, 217, 338, 386, 455, 491, 527
Alw21I GWGCWC 2 cut(s) 209, 508
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 2 cut(s) 205, 504
AlwI GGATC 1 cut(s) 418
AoxI GGCC 1 cut(s) 478
ApaLI GTGCAC 2 cut(s) 205, 504
ApeKI GCWGC 8 cut(s) 16, 117, 128, 149, 335, 364, 428, 452
ApoI RAATTY 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 479
AsuHPI GGTGA 2 cut(s) 404, 538
BaeGI GKGCMC 3 cut(s) 180, 209, 508
BauI CACGAG 1 cut(s) 202
Bbv12I GWGCWC 2 cut(s) 209, 508
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 8 cut(s) 3, 104, 115, 161, 347, 351, 415, 464
BccI CCATC 4 cut(s) 69, 114, 198, 220
BceAI ACGGC 2 cut(s) 158, 268
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 176, 210
BcoDI GTCTC 1 cut(s) 371
BfmI CTRYAG 2 cut(s) 243, 474
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 2 cut(s) 231, 471
BmsI GCATC 1 cut(s) 385
Bpu10I CCTNAGC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 400
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 1 cut(s) 106
BseMII CTCAG 2 cut(s) 227, 280
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 2 cut(s) 251, 254
BseSI GKGCMC 3 cut(s) 180, 209, 508
BseXI GCAGC 8 cut(s) 3, 104, 115, 161, 347, 351, 415, 464
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 209, 508
BsiSI CCGG 2 cut(s) 32, 233
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 1 cut(s) 480
Bsp1286I GDGCHC 3 cut(s) 180, 209, 508
Bsp143I GATC 1 cut(s) 410
BspACI CCGC 6 cut(s) 90, 131, 221, 270, 273, 431
BspANI GGCC 1 cut(s) 480
BspCNI CTCAG 2 cut(s) 226, 279
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 231, 471
BspMAI CTGCAG 2 cut(s) 247, 478
BspPI GGATC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 1 cut(s) 513
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 1 cut(s) 410
BssNAI GTATAC 2 cut(s) 12, 443
BssSI CACGAG 1 cut(s) 202
BssT1I CCWWGG 1 cut(s) 400
Bst1107I GTATAC 2 cut(s) 12, 443
Bst2BI CACGAG 1 cut(s) 202
BstC8I GCNNGC 4 cut(s) 33, 154, 219, 478
BstDEI CTNAG 4 cut(s) 213, 266, 500, 555
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 1 cut(s) 413
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 1 cut(s) 410
BstMWI GCNNNNNNNGC 6 cut(s) 32, 162, 184, 227, 242, 344
BstSFI CTRYAG 2 cut(s) 243, 474
BstSLI GKGCMC 3 cut(s) 180, 209, 508
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 8 cut(s) 3, 104, 115, 161, 347, 351, 415, 464
BstZ17I GTATAC 2 cut(s) 12, 443
BsuRI GGCC 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 506
Cac8I GCNNGC 4 cut(s) 33, 154, 219, 478
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 479
Csp6I GTAC 1 cut(s) 52
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 4 cut(s) 213, 266, 500, 555
DpnI GATC 1 cut(s) 412
DpnII GATC 1 cut(s) 410
DraI TTTAAA 1 cut(s) 169
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FaiI YATR 4 cut(s) 12, 443, 521, 551
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FauI CCCGC 2 cut(s) 97, 124
FblI GTMKAC 3 cut(s) 11, 249, 442
FokI GGATG 1 cut(s) 93
HaeIII GGCC 1 cut(s) 480
HapII CCGG 2 cut(s) 32, 233
HindIII AAGCTT 1 cut(s) 163
HpaII CCGG 2 cut(s) 32, 233
HphI GGTGA 2 cut(s) 404, 538
Hpy166II GTNNAC 5 cut(s) 12, 207, 250, 443, 506
Hpy188III TCNNGA 2 cut(s) 291, 330
Hpy8I GTNNAC 5 cut(s) 12, 207, 250, 443, 506
Hpy99I CGWCG 2 cut(s) 10, 198
HpyCH4V TGCA 6 cut(s) 19, 207, 245, 367, 476, 506
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 162, 184, 227, 242, 344
HpyF3I CTNAG 4 cut(s) 213, 266, 500, 555
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Kzo9I GATC 1 cut(s) 410
LmnI GCTCC 3 cut(s) 235, 332, 475
LpnPI CCDG 9 cut(s) 45, 52, 203, 246, 282, 315, 462, 486, 494
Lsp1109I GCAGC 8 cut(s) 3, 104, 115, 161, 347, 351, 415, 464
LweI GCATC 1 cut(s) 385
MaeIII GTNAC 1 cut(s) 544
MalI GATC 1 cut(s) 412
MboI GATC 1 cut(s) 410
MhlI GDGCHC 3 cut(s) 180, 209, 508
MluCI AATT 3 cut(s) 43, 69, 464
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 5 cut(s) 250, 269, 272, 275, 492
MreI CGCCGGCG 1 cut(s) 31
MroNI GCCGGC 1 cut(s) 31
MseI TTAA 1 cut(s) 168
MspA1I CMGCKG 2 cut(s) 217, 270
MspI CCGG 2 cut(s) 32, 233
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 6 cut(s) 32, 162, 184, 227, 242, 344
NaeI GCCGGC 1 cut(s) 33
NdeII GATC 1 cut(s) 410
NgoMIV GCCGGC 1 cut(s) 31
NlaIV GGNNCC 2 cut(s) 231, 471
NmuCI GTSAC 1 cut(s) 544
PdiI GCCGGC 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 231, 471
PspPI GGNCC 1 cut(s) 479
PstI CTGCAG 2 cut(s) 247, 478
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SaqAI TTAA 1 cut(s) 168
Sau3AI GATC 1 cut(s) 410
Sau96I GGNCC 1 cut(s) 479
SbfI CCTGCAGG 1 cut(s) 478
SdaI CCTGCAGG 1 cut(s) 478
SduI GDGCHC 3 cut(s) 180, 209, 508
SetI ASST 8 cut(s) 167, 219, 242, 340, 388, 457, 493, 529
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 2 cut(s) 243, 474
SgrAI CRCCGGYG 1 cut(s) 31
Sse8387I CCTGCAGG 1 cut(s) 478
Sse9I AATT 3 cut(s) 43, 69, 464
SsiI CCGC 6 cut(s) 90, 131, 221, 270, 273, 431
StyI CCWWGG 1 cut(s) 400
TaqI TCGA 1 cut(s) 290
TasI AATT 3 cut(s) 43, 69, 464
TauI GCSGC 2 cut(s) 273, 276
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 8 cut(s) 16, 117, 128, 149, 335, 364, 428, 452
Tsp45I GTSAC 1 cut(s) 544
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 1 cut(s) 513
VneI GTGCAC 2 cut(s) 205, 504
XapI RAATTY 1 cut(s) 69
XmiI GTMKAC 3 cut(s) 11, 249, 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.