RLG00000004720

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015850
Physical Location & Seq
Reverse (-)
61697185 .. 61699765
2581 bp
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UTR
Exon/CDS
Intron
RLM00000004720

Sequence Viewer

Length: 564 bp
ATGATGGCGTCGGCCACTTTCAATTTCTCCAGGGTTTGCTTGTACCAGCACCACTCCACAGTTCCCAGATCCAAATCTTCTGCATTAGTTTCAAATTCTAAACCTTTTCGCTGCGATTTTATCGCGGGCAATGAAAAACTGATACTGGATAATGCGACTGGTCTCAGAACCAAAGCCAAGATAGCTCCCAATCCCCGTCGTCGGTTTTCGTGCACTTCTAATCTGGCGGAGTATGCCCAGACTACCTCTGCCGTCTATGGATTCCTCCTCCTCAGCGGTGGCTTCTTTGCCTATACTAGGACAGGAAGCAAAGGGTCACTTCTAGGAGGCGTTTCAGGGGCAGCTGTAATGGCCACTGCGTACTATCTGATGAAAACACCAGAGACAGAAGCTCTTGGTGATGCCCTTGGCTTTGGATCAGCATTCCTTTTTGCTTCTGTATTTGGTATCCGATTAGCAGCTACTCGGAAATTTGCTCCTGCAGGCCCTCTCTTGGCTCTTTCCCTCAGTGCACTGGCTGTTTTTATAACAGCATACCTTCAAGATAGTGTCACTACTCTTTAG

Protein Analysis

188

Amino Acids

19.84

Weight (kDa)

9.86

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 83 - 175 3.8e-16 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33265
fragaria_vesca FvH4_5g10080 FvH4_5g10080
malus_domestica MD06G1128100.v1.1
prunus_persica Prupe.5G137900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0188191
rosa_laevigata RLG00000004720
rosa_multiflora Rmu_sc0004001.1_g000016 Rmu_sc0015196.1_g000001
rosa_roxburghii Rroxscaffold_3G00266680
rosa_rugosa Rorug06G0489000
rosa_samantha Rh7AG093300 Rh7BG094800 Rh7CG094600 Rh7DG095200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AccII CGCG 1 cut(s) 125
AciI CCGC 3 cut(s) 125, 227, 276
AclWI GGATC 2 cut(s) 63, 424
AcoI YGGCCR 2 cut(s) 12, 351
AcsI RAATTY 2 cut(s) 94, 470
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 2 cut(s) 44, 362
AfiI CCNNNNNNNGG 3 cut(s) 201, 297, 493
AgsI TTSAA 3 cut(s) 22, 93, 542
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 4 cut(s) 185, 344, 392, 461
AluI AGCT 4 cut(s) 185, 344, 392, 461
Alw21I GWGCWC 2 cut(s) 215, 514
Alw26I GTCTC 2 cut(s) 167, 377
Alw44I GTGCAC 2 cut(s) 211, 510
AlwI GGATC 2 cut(s) 63, 424
AoxI GGCC 3 cut(s) 12, 351, 484
ApaLI GTGCAC 2 cut(s) 211, 510
ApeKI GCWGC 3 cut(s) 111, 341, 458
ApoI RAATTY 2 cut(s) 94, 470
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 410
BaeGI GKGCMC 2 cut(s) 215, 514
BalI TGGCCA 1 cut(s) 353
Bbv12I GWGCWC 2 cut(s) 215, 514
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 3 cut(s) 98, 353, 470
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 31
BciVI GTATCC 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 167, 377
BfaI CTAG 2 cut(s) 297, 323
BfmI CTRYAG 1 cut(s) 480
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 3 cut(s) 112, 342, 459
BlsI GCNGC 3 cut(s) 113, 343, 460
Bme1390I CCNGG 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 485
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 13
Bpu10I CCTNAGC 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 30, 406
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 297, 493
Bse1I ACTGG 3 cut(s) 150, 163, 519
Bse3DI GCAATG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 30, 406
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 201, 297, 493
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 3 cut(s) 178, 286, 520
BseNI ACTGG 3 cut(s) 150, 163, 519
BseRI GAGGAG 2 cut(s) 257, 260
BseSI GKGCMC 2 cut(s) 215, 514
BseXI GCAGC 3 cut(s) 98, 353, 470
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 3 cut(s) 14, 353, 486
BsiHKAI GWGCWC 2 cut(s) 215, 514
BslI CCNNNNNNNGG 3 cut(s) 201, 297, 493
BsmAI GTCTC 2 cut(s) 167, 377
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 3 cut(s) 14, 353, 486
Bso31I GGTCTC 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 215, 514
Bsp143I GATC 2 cut(s) 68, 416
BspACI CCGC 3 cut(s) 125, 227, 276
BspANI GGCC 3 cut(s) 14, 353, 486
BspCNI CTCAG 3 cut(s) 177, 285, 519
BspFNI CGCG 1 cut(s) 125
BspMAI CTGCAG 1 cut(s) 484
BspPI GGATC 2 cut(s) 63, 424
BspTNI GGTCTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 136
BsrI ACTGG 3 cut(s) 150, 163, 519
BssECI CCNNGG 2 cut(s) 30, 406
BssMI GATC 2 cut(s) 68, 416
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 61
BstACI GRCGYC 1 cut(s) 8
BstC8I GCNNGC 2 cut(s) 127, 484
BstDEI CTNAG 3 cut(s) 164, 272, 506
BstENI CCTNNNNNAGG 1 cut(s) 295
BstFNI CGCG 1 cut(s) 125
BstKTI GATC 2 cut(s) 71, 419
BstMAI GTCTC 2 cut(s) 167, 377
BstMBI GATC 2 cut(s) 68, 416
BstMWI GCNNNNNNNGC 3 cut(s) 182, 233, 350
BstNI CCWGG 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 29
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 2 cut(s) 215, 514
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 3 cut(s) 98, 353, 470
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 3 cut(s) 14, 353, 486
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 354, 512, 514
Cac8I GCNNGC 2 cut(s) 127, 484
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 2 cut(s) 43, 361
CviQI GTAC 2 cut(s) 43, 361
DdeI CTNAG 3 cut(s) 164, 272, 506
DpnI GATC 2 cut(s) 70, 418
DpnII GATC 2 cut(s) 68, 416
EaeI YGGCCR 2 cut(s) 12, 351
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 406
Eco31I GGTCTC 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FaiI YATR 5 cut(s) 234, 258, 294, 527, 535
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FauI CCCGC 1 cut(s) 118
Fnu4HI GCNGC 3 cut(s) 112, 342, 459
Fsp4HI GCNGC 3 cut(s) 112, 342, 459
FspBI CTAG 2 cut(s) 297, 323
GluI GCNGC 3 cut(s) 112, 342, 459
GsuI CTGGAG 1 cut(s) 13
HaeIII GGCC 3 cut(s) 14, 353, 486
Hin1I GRCGYC 1 cut(s) 8
HinfI GANTC 1 cut(s) 261
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 213, 512
Hpy188I TCNGA 4 cut(s) 167, 369, 452, 468
Hpy188III TCNNGA 1 cut(s) 542
Hpy8I GTNNAC 2 cut(s) 213, 512
Hpy99I CGWCG 3 cut(s) 13, 201, 204
HpyAV CCTTC 1 cut(s) 548
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 4 cut(s) 83, 213, 482, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 233, 350
HpyF3I CTNAG 3 cut(s) 164, 272, 506
Hsp92I GRCGYC 1 cut(s) 8
Kzo9I GATC 2 cut(s) 68, 416
LmnI GCTCC 2 cut(s) 190, 481
Lsp1109I GCAGC 3 cut(s) 98, 353, 470
LweI GCATC 1 cut(s) 391
MaeI CTAG 2 cut(s) 297, 323
MaeIII GTNAC 2 cut(s) 315, 550
MalI GATC 2 cut(s) 70, 418
MboI GATC 2 cut(s) 68, 416
MboII GAAGA 1 cut(s) 69
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 2 cut(s) 215, 514
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 3 cut(s) 22, 94, 470
MluNI TGGCCA 1 cut(s) 353
MnlI CCTC 7 cut(s) 256, 275, 278, 281, 320, 498, 515
Mox20I TGGCCA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 2 cut(s) 276, 344
MspR9I CCNGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 422
MvaI CCWGG 1 cut(s) 31
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 182, 233, 350
NdeII GATC 2 cut(s) 68, 416
NmuCI GTSAC 2 cut(s) 315, 550
PctI GAATGC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 3 cut(s) 113, 343, 460
PsiI TTATAA 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 29
PspPI GGNCC 1 cut(s) 485
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 2 cut(s) 44, 362
RsaNI GTAC 2 cut(s) 43, 361
SatI GCNGC 3 cut(s) 112, 342, 459
Sau3AI GATC 2 cut(s) 68, 416
Sau96I GGNCC 1 cut(s) 485
SbfI CCTGCAGG 1 cut(s) 484
ScrFI CCNGG 1 cut(s) 31
SdaI CCTGCAGG 1 cut(s) 484
SduI GDGCHC 2 cut(s) 215, 514
SetI ASST 7 cut(s) 106, 187, 248, 346, 394, 463, 540
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 480
Sse8387I CCTGCAGG 1 cut(s) 484
Sse9I AATT 3 cut(s) 22, 94, 470
SsiI CCGC 3 cut(s) 125, 227, 276
SspMI CTAG 2 cut(s) 297, 323
StyD4I CCNGG 1 cut(s) 29
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 61
TasI AATT 3 cut(s) 22, 94, 470
TfiI GAWTC 1 cut(s) 261
TscAI CASTG 3 cut(s) 361, 514, 519
TseFI GTSAC 2 cut(s) 315, 550
TseI GCWGC 3 cut(s) 111, 341, 458
Tsp45I GTSAC 2 cut(s) 315, 550
TspDTI ATGAA 2 cut(s) 147, 386
TspRI CASTG 3 cut(s) 361, 514, 519
VneI GTGCAC 2 cut(s) 211, 510
XagI CCTNNNNNAGG 1 cut(s) 295
XapI RAATTY 2 cut(s) 94, 470
XspI CTAG 2 cut(s) 297, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.