MD06G1129100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012434
Physical Location & Seq
Reverse (-)
27270868 .. 27272511
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1129100.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCCCTGCAACTGCAGGCACCCACCACAAGAAGCCACCTAGCGCCACCGTCTCCGTTGGCAGCTCCGAAGGGCAATGCCGGCCTCCGACAACCGCCCGATCGTTTCGCGCTAAAATCATCCTTCTTCTCTTCGTCACACGACCTCTTATTGCTTTCTCCCAAGCACAGGCCTCGTGCTTCTTCCGCCGCACCCAAGTTTTCCATGCGCGTTGCCTCCAAAGGAGCATATATTTGTCGCGATTGCGGGTATATATACAACGACAGAACTCCCTTTGAGAAGTTACCTGACAAGTATTTCTGCCCTGTTTGTGGTGCTCCGAAACGGAGGTTTAGGCCATACCAGCCTCCGGTGACCAAGGACGCCAACAGCAAAGACGTCCGAAAGCAGCGAAAAGCGCAGTTGCAGAGAGAAGAAACAATCGGGAAGGCGCTGCCTATTGCTGTCGCCGTCGGAGCTGTGGCGCTTGTTGGATTGTACTTCTACATCAATGTCGGCTTTCAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubredoxin

Protein Analysis

170

Amino Acids

18.63

Weight (kDa)

10.18

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RUBY_RBDX PF21349 77 - 111 6e-06 Rubrerythrin, rubredoxin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51010
fragaria_vesca FvH4_5g09700
malus_domestica MD06G1129100.v1.1 MD14G1146000.v1.1
prunus_persica Prupe.5G140400_v2.0.a1 Prupe.5G140400_v2.0.a1 Prupe.5G140400_v2.0.a1
pyrus_communis pycom14g12100
rosa_chinensis RchiOBHm_Chr7g0188541
rosa_laevigata RLG00000004685
rosa_multiflora Rmu_sc0001576.1_g000010 Rmu_sc0016427.1_g000010
rosa_roxburghii Rroxscaffold_3G00266270
rosa_rugosa Rorug06G0491100
rosa_samantha Rh7AG096000 Rh7BG097800 Rh7CG098800 Rh7DG098000
rosa_wichuraiana Rw7G008290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 3 cut(s) 110, 210, 240
AciI CCGC 4 cut(s) 95, 186, 189, 246
AcyI GRCGYC 2 cut(s) 363, 378
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 3 cut(s) 168, 311, 349
AluBI AGCT 2 cut(s) 65, 458
AluI AGCT 2 cut(s) 65, 458
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 57
AoxI GGCC 4 cut(s) 3, 82, 170, 335
ApeKI GCWGC 3 cut(s) 62, 388, 433
AspLEI GCGC 6 cut(s) 46, 112, 210, 400, 433, 466
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 364
BanI GGYRCC 1 cut(s) 19
BauI CACGAG 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 74, 400, 420
BceAI ACGGC 1 cut(s) 434
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 14
BfoI RGCGCY 3 cut(s) 47, 434, 467
BisI GCNGC 4 cut(s) 63, 189, 389, 434
BlsI GCNGC 4 cut(s) 64, 190, 390, 435
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 363, 378
BsaJI CCNNGG 1 cut(s) 357
BsaWI WCCGGW 1 cut(s) 349
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 311, 349
Bse118I RCCGGY 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 3 cut(s) 168, 311, 349
BseMI GCAATG 1 cut(s) 82
BseXI GCAGC 3 cut(s) 74, 400, 420
Bsh1236I CGCG 3 cut(s) 110, 210, 240
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 4 cut(s) 5, 84, 172, 337
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 2 cut(s) 81, 350
BslI CCNNNNNNNGG 3 cut(s) 168, 311, 349
BsmAI GTCTC 1 cut(s) 57
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 4 cut(s) 5, 84, 172, 337
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 100
Bsp68I TCGCGA 1 cut(s) 240
BspACI CCGC 4 cut(s) 95, 186, 189, 246
BspANI GGCC 4 cut(s) 5, 84, 172, 337
BspFNI CGCG 3 cut(s) 110, 210, 240
BspLI GGNNCC 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 18
BspT107I GGYRCC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 2 cut(s) 363, 378
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 136
BstACI GRCGYC 2 cut(s) 363, 378
BstC8I GCNNGC 2 cut(s) 18, 82
BstEII GGTNACC 1 cut(s) 352
BstF5I GGATG 1 cut(s) 119
BstFNI CGCG 3 cut(s) 110, 210, 240
BstH2I RGCGCY 3 cut(s) 47, 434, 467
BstHHI GCGC 6 cut(s) 46, 112, 210, 400, 433, 466
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 1 cut(s) 100
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 5 cut(s) 81, 185, 343, 397, 455
BstPI GGTNACC 1 cut(s) 352
BstSFI CTRYAG 1 cut(s) 14
BstUI CGCG 3 cut(s) 110, 210, 240
BstV1I GCAGC 3 cut(s) 74, 400, 420
BsuRI GGCC 4 cut(s) 5, 84, 172, 337
BtsCI GGATG 1 cut(s) 119
BtuMI TCGCGA 1 cut(s) 240
Cac8I GCNNGC 2 cut(s) 18, 82
CfoI GCGC 6 cut(s) 46, 112, 210, 400, 433, 466
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 371
Csp6I GTAC 1 cut(s) 478
CviAII CATG 1 cut(s) 205
CviJI RGCY 9 cut(s) 5, 36, 65, 84, 172, 337, 346, 458, 498
CviKI_1 RGCY 9 cut(s) 5, 36, 65, 84, 172, 337, 346, 458, 498
CviQI GTAC 1 cut(s) 478
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
EciI GGCGGA 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 172
Eco91I GGTNACC 1 cut(s) 352
EcoO65I GGTNACC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 1 cut(s) 208
FaiI YATR 7 cut(s) 206, 229, 231, 252, 254, 256, 340
FatI CATG 1 cut(s) 204
FauI CCCGC 1 cut(s) 239
Fnu4HI GCNGC 4 cut(s) 63, 189, 389, 434
FokI GGATG 1 cut(s) 106
Fsp4HI GCNGC 4 cut(s) 63, 189, 389, 434
FspBI CTAG 1 cut(s) 41
GlaI GCGC 6 cut(s) 45, 111, 209, 399, 432, 465
GluI GCNGC 4 cut(s) 63, 189, 389, 434
HaeII RGCGCY 3 cut(s) 47, 434, 467
HaeIII GGCC 4 cut(s) 5, 84, 172, 337
HapII CCGG 2 cut(s) 81, 350
HgaI GACGC 1 cut(s) 371
HhaI GCGC 6 cut(s) 46, 112, 210, 400, 433, 466
Hin1I GRCGYC 2 cut(s) 363, 378
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 6 cut(s) 44, 110, 208, 398, 431, 464
HinP1I GCGC 6 cut(s) 44, 110, 208, 398, 431, 464
HpaII CCGG 2 cut(s) 81, 350
HphI GGTGA 1 cut(s) 364
Hpy188I TCNGA 5 cut(s) 69, 89, 321, 383, 455
Hpy188III TCNNGA 2 cut(s) 239, 424
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 3 cut(s) 64, 133, 421
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 3 cut(s) 10, 16, 406
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 185, 343, 397, 455
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 2 cut(s) 363, 378
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 6 cut(s) 44, 110, 208, 398, 431, 464
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Kzo9I GATC 1 cut(s) 100
LmnI GCTCC 4 cut(s) 70, 224, 322, 455
LpnPI CCDG 9 cut(s) 2, 20, 94, 154, 300, 318, 356, 363, 488
Lsp1109I GCAGC 3 cut(s) 74, 400, 420
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 3 cut(s) 135, 282, 352
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 4 cut(s) 118, 123, 174, 425
MhlI GDGCHC 1 cut(s) 319
MmeI TCCRAC 3 cut(s) 112, 433, 451
MnlI CCTC 6 cut(s) 95, 155, 183, 226, 321, 357
MroNI GCCGGC 1 cut(s) 80
MspI CCGG 2 cut(s) 81, 350
MvnI CGCG 3 cut(s) 110, 210, 240
MwoI GCNNNNNNNGC 5 cut(s) 81, 185, 343, 397, 455
NaeI GCCGGC 1 cut(s) 82
NdeII GATC 1 cut(s) 100
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 135, 352
NruI TCGCGA 1 cut(s) 240
PceI AGGCCT 1 cut(s) 172
PdiI GCCGGC 1 cut(s) 82
PkrI GCNGC 4 cut(s) 64, 190, 390, 435
Ple19I CGATCG 1 cut(s) 103
PspEI GGTNACC 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 18
PvuI CGATCG 1 cut(s) 103
RruI TCGCGA 1 cut(s) 240
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
SatI GCNGC 4 cut(s) 63, 189, 389, 434
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 1 cut(s) 4
SduI GDGCHC 1 cut(s) 319
SetI ASST 7 cut(s) 42, 67, 147, 289, 332, 381, 460
SfcI CTRYAG 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 4 cut(s) 95, 186, 189, 246
SspMI CTAG 1 cut(s) 41
StuI AGGCCT 1 cut(s) 172
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 1 cut(s) 381
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 191
TseFI GTSAC 2 cut(s) 135, 352
TseI GCWGC 3 cut(s) 62, 388, 433
Tsp45I GTSAC 2 cut(s) 135, 352
TspGWI ACGGA 2 cut(s) 45, 340
XspI CTAG 1 cut(s) 41
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.