MD06G1131800.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011770
Physical Location & Seq
Forward (+)
27560230 .. 27565609
5380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1131800.v1.1.491

Sequence Viewer

Length: 2421 bp
ATGCATTTCGCAAAGCTCGATGATTCGCCTATGTTCCGCCAACAGATACAAGGCTTGGAGGAAAGTGCAGAGTCACTGAGGGGAAGATGTTATAAGTTCTACAAAGGATGCCGAAAGTACACAGAAGGACTTGGAGAAGCGTATGATGGTGAAATCGCTTTTATAACTGCCCTCGAAAGTTTTGGGGGAGGCCACAATGATCCTATTTTTTCAGCTTTAGGAGGCCAGGTTATGTCCAGATTCACAATTGCATTAAGAGAAATCGCGACGTACAAGGAAGTTTTGCGTTCACAGATAGAGCACATGGTAAATGATAGGCTGGTGAACATTGTCCATGTTGACCTCCATGAAGTCAAGGAATCCCGAAAGCGTTTTGACAAGACTTCACTTGTATATGATCAGGCACGCGAGAAGTTTCTGTCATTGAGGAAGAGCACGAGGATGGATATAGCTGCTAGTATAGAGGAGGAACTGCAGAATGCAAGATCCTCGTTTGAGCAAGCCCGCTTCAGCCTGGTTGCTGTTCTTTCTGATATCGAGGCCAAAAAGAGGTTTGAGTTTCTGGAAGCTGTTAGCGGGGTGATGGACGCTCATCTTCGTTACTTCAAACAGAGTTATGAGTTACTGCATGCAATGGAACCCTACATTCATCAGGTTTTGGCTTACGCGCAACAGACACGAGAAAGTTGCAACCAGGAACAGATGTGTCTCAACGAAAGGATACAAGAGTACAAAAAGCAGATTGATAGGGAAAGTACACAGTCCTTGAGCGGTATCCATGGTTCTACCAAAGGAGATGGCCCGCAACCTTTCTCTAGGAAATCACATAAAGTGATCGAGGAAGTGATGCAATCTGCTGCAAAGGGGAAGGTACAAACCATTCGGCAAGGATATCTATCAAAGCGTTCCTCCAATTTGAGAGGTGACTGGAAAAGAAGGTATTTCATTCTTGATAGTCGAGGGATGCTGTACTACTATCGCAAACCATGGAGTTGGCCATCTTATGGTAGTCCATCTTCTCCACAGAAGAATAATTCCTCTGAAAATGGCTCCGGGCTTTTGAGTCGATGGCTTTCATCTCATTACCATGGCGGTGTACATGATGAGAAATCTGTTGCTAGCCACACTGTGAACTTGCTGACATCAACGATAAAGGTTGATGCAGACCAGACGGACTTAAGGTTCTGCTTCAGAATTATATCACCTGCTAAAATCTACACTTTACAGGCAGAGAATGCACTTGACCAAATGGATTGGATTGAGAAAATAACTGGAGTAATAGCTTCATTGTTGAGTTTCCAGACACCTGAGAGGCGTCTCTCAACTAGCCCTACAGGAAGCGACGATCAATTCTCTGGAAACGAGGGCAACTTATTAGAAAGCGCTTCTGATGCTGAGCAGACAGCTATCCAGGAACTCTCACCTAAGATCACAGCTTACAATTATCTACGAATCTCTAAAAGTATGCAGCCGCACAAGCTCAAAGAGAAAAGTGAGAAACCAATTGACATACTGAGAAGAGTGTGCGGTAATGACAAATGTGCTGATTGTGGTGCACCTGAACCAGATTGGGCATCTTTGAACCTTGGAATTCTTATTTGCATTGAATGCTCTGGTGTTCATCGTAATCTTGGTGTACATGTATCAAAGGTTCGATCACTAACACTTGATGTGAAAGTATGGGAACCTTCTGTCTTAACCTTGTTTCAATCACTGGGAAATGCTTTTGCAAACTCGATTTGGGAGGAGATGTTGTATTCAAGAAGTAGTTTGCAGGCTGATAACATGCCTATAGGTTTCTCCAAGGCTGATAGGAATGAGCCGTTTCTTATGATAAAGCCATGTAATGACGATCTTAATTCGACAAAGGAGAGGTATATTCAAGCAAAGTATGCAGAAAAACTTTTTGTTCGTCGTACAAAAAACAATCACAAACTTCATGTCATGGGGCAACAGTTGTGCAAAAGCGTCCGTGCTAATGACAAGAAAGCAGTTTACCGCTATATCATTGGTTATGAAGCAGATGTTAATTCTAGATCTGTGCAAGCATTAGATGATATTTCTGGAGACAAGCCCTCCTCGAGTAACTTAAATTCCGTCACTCAAACTGAAATTCAGCCTGCAGAAGACACTGCTGAAGGCTGTTCCCTGCTTCACCTAGCCTGCCAAAGTGCCGATATTGGCATGGTAGAGCTCCTTTTACAGTACGGTGCCAACGTAAAAGCTACTGATTCAAAAGGTCAAACACCACTTCATTATTCCGTTATAAGAGGGAATATTGCTATTGCAAAGTTGCTTCTAACAAGGGGAGCTGATCCAAAAGCCACTGACAGAGAAGGTAAAACACCAAGTGAGCTTGTATCAGACTCAGCCTCCAAAGACAATGGGATGCTGGCTCTTTTAACAAACTCCGGCAGATGA

Protein Analysis

807

Amino Acids

90.65

Weight (kDa)

8.2

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 6 - 234 5.4e-38 BAR domain of APPL family
BAR PF03114 75 - 216 3.4e-14 BAR domain
PH PF00169 294 - 427 8.1e-11 PH domain
ArfGap PF01412 502 - 637 3.2e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 710 - 777 2.4e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 718 - 767 1.6e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 733 - 785 2.6e-09 Ankyrin repeats (many copies)
Ank PF00023 747 - 777 1e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011770)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61980
fragaria_vesca FvH4_5g09370 FvH4_5g09370 FvH4_5g09370
malus_domestica MD06G1131800.v1.1 MD14G1148100.v1.1
prunus_persica Prupe.5G142800_v2.0.a1
pyrus_communis pycom06g12340 pycom14g12290
rosa_chinensis RchiOBHm_Chr7g0188801
rosa_laevigata RLG00000004658
rosa_multiflora Rmu_sc0003642.1_g000001
rosa_roxburghii Rroxscaffold_3G00266010
rosa_rugosa Rorug06G0494100
rosa_samantha Rh7AG098800 Rh7BG100500 Rh7CG102000 Rh7DG100600
rosa_wichuraiana Rw7G008510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 93, 164, 2267
AarI CACCTGC 1 cut(s) 1213
Acc36I ACCTGC 1 cut(s) 1213
AccB1I GGYRCC 1 cut(s) 2210
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccBSI CCGCTC 1 cut(s) 771
AccII CGCG 3 cut(s) 266, 408, 668
AciI CCGC 9 cut(s) 37, 505, 576, 771, 803, 1092, 1472, 1527, 1999
AclWI GGATC 3 cut(s) 194, 480, 2309
AcoI YGGCCR 1 cut(s) 995
AcsI RAATTY 3 cut(s) 1590, 2092, 2112
AcuI CTGAAG 3 cut(s) 493, 1174, 2157
AcyI GRCGYC 1 cut(s) 1315
AdeI CACNNNGTG 2 cut(s) 1129, 2351
AfeI AGCGCT 1 cut(s) 1384
AfiI CCNNNNNNNGG 2 cut(s) 549, 1004
AflII CTTAAG 1 cut(s) 1177
AflIII ACRYGT 1 cut(s) 1639
AgsI TTSAA 7 cut(s) 607, 1582, 1607, 1709, 1761, 1883, 2235
AjnI CCWGG 4 cut(s) 225, 513, 693, 1410
AloI GAACNNNNNNTCC 2 cut(s) 1166, 1198
Alw21I GWGCWC 4 cut(s) 303, 437, 1558, 2196
Alw26I GTCTC 3 cut(s) 713, 1322, 2061
Alw44I GTGCAC 1 cut(s) 1554
AlwI GGATC 3 cut(s) 194, 480, 2309
Ama87I CYCGRG 1 cut(s) 2080
Aor51HI AGCGCT 1 cut(s) 1384
AoxI GGCC 5 cut(s) 190, 223, 540, 799, 995
ApaLI GTGCAC 1 cut(s) 1554
ApeKI GCWGC 3 cut(s) 452, 857, 1468
ApoI RAATTY 3 cut(s) 1590, 2092, 2112
ArsI GACNNNNNNTTYG 2 cut(s) 1926, 1958
AspLEI GCGC 2 cut(s) 670, 1385
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 1 cut(s) 1054
AsuHPI GGTGA 7 cut(s) 161, 334, 592, 935, 1194, 1413, 2147
AsuNHI GCTAGC 1 cut(s) 1118
AvaI CYCGRG 1 cut(s) 2080
BaeGI GKGCMC 1 cut(s) 1558
BaeI ACNNNNGTAYC 2 cut(s) 713, 746
BalI TGGCCA 1 cut(s) 997
BanI GGYRCC 1 cut(s) 2210
BanII GRGCYC 1 cut(s) 2196
BarI GAAGNNNNNNTAC 2 cut(s) 1000, 1032
BauI CACGAG 2 cut(s) 436, 678
BbsI GAAGAC 1 cut(s) 2133
Bbv12I GWGCWC 4 cut(s) 303, 437, 1558, 2196
BbvI GCAGC 3 cut(s) 439, 844, 1480
BccI CCATC 7 cut(s) 140, 436, 577, 791, 1006, 1021, 1062
BceAI ACGGC 1 cut(s) 1807
BcgI CGANNNNNNTGC 4 cut(s) 471, 505, 669, 703
BciT130I CCWGG 4 cut(s) 227, 515, 695, 1412
BciVI GTATCC 2 cut(s) 714, 785
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 1 cut(s) 1054
BcoDI GTCTC 3 cut(s) 713, 1322, 2061
BfaI CTAG 6 cut(s) 456, 816, 1119, 1326, 2034, 2159
BfmI CTRYAG 4 cut(s) 473, 1332, 1791, 2121
BfoI RGCGCY 1 cut(s) 1386
BfrI CTTAAG 1 cut(s) 1177
BfuAI ACCTGC 1 cut(s) 1213
BfuI GTATCC 2 cut(s) 714, 785
BglII AGATCT 1 cut(s) 2036
BisI GCNGC 4 cut(s) 453, 858, 1469, 1472
BlpI GCTNAGC 1 cut(s) 1395
BlsI GCNGC 4 cut(s) 454, 859, 1470, 1473
Bme1390I CCNGG 5 cut(s) 227, 515, 695, 1054, 1412
BmeT110I CYCGRG 1 cut(s) 2080
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 4 cut(s) 639, 1051, 1686, 2212
BmrFI CCNGG 5 cut(s) 227, 515, 695, 1054, 1412
BmrI ACTGGG 1 cut(s) 1724
BmsI GCATC 7 cut(s) 98, 837, 954, 1150, 1381, 1583, 2379
BmtI GCTAGC 1 cut(s) 1122
BmuI ACTGGG 1 cut(s) 1724
BpiI GAAGAC 1 cut(s) 2133
BpmI CTGGAG 2 cut(s) 1293, 2085
Bpu1102I GCTNAGC 1 cut(s) 1395
BpuEI CTTGAG 1 cut(s) 787
BpuMI CCSGG 1 cut(s) 1054
BsaBI GATNNNNATC 1 cut(s) 895
BsaHI GRCGYC 1 cut(s) 1315
BsaJI CCNNGG 5 cut(s) 778, 986, 1087, 1585, 1803
Bsc4I CCNNNNNNNGG 2 cut(s) 549, 1004
Bse1I ACTGG 3 cut(s) 932, 1276, 1719
Bse3DI GCAATG 1 cut(s) 639
Bse8I GATNNNNATC 1 cut(s) 895
BseBI CCWGG 4 cut(s) 227, 515, 695, 1412
BseDI CCNNGG 5 cut(s) 778, 986, 1087, 1585, 1803
BseGI GGATG 4 cut(s) 113, 447, 969, 2394
BseJI GATNNNNATC 1 cut(s) 895
BseLI CCNNNNNNNGG 2 cut(s) 549, 1004
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 5 cut(s) 68, 1299, 1386, 1505, 2382
BseNI ACTGG 3 cut(s) 932, 1276, 1719
BseRI GAGGAG 3 cut(s) 479, 1760, 2068
BseSI GKGCMC 1 cut(s) 1558
BseXI GCAGC 3 cut(s) 439, 844, 1480
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 3 cut(s) 266, 408, 668
BshFI GGCC 5 cut(s) 192, 225, 542, 801, 997
BshNI GGYRCC 1 cut(s) 2210
BsiHKAI GWGCWC 4 cut(s) 303, 437, 1558, 2196
BsiHKCI CYCGRG 1 cut(s) 2080
BsiSI CCGG 2 cut(s) 1053, 2412
BslI CCNNNNNNNGG 2 cut(s) 549, 1004
BsmAI GTCTC 3 cut(s) 713, 1322, 2061
BsmBI CGTCTC 1 cut(s) 1322
BsmI GAATGC 3 cut(s) 484, 1240, 1613
BsnI GGCC 5 cut(s) 192, 225, 542, 801, 997
BsoBI CYCGRG 1 cut(s) 2080
Bsp1286I GDGCHC 4 cut(s) 303, 437, 1558, 2196
Bsp1407I TGTACA 2 cut(s) 1096, 1636
Bsp1720I GCTNAGC 1 cut(s) 1395
Bsp19I CCATGG 3 cut(s) 778, 986, 1087
Bsp68I TCGCGA 1 cut(s) 266
BspACI CCGC 9 cut(s) 37, 505, 576, 771, 803, 1092, 1472, 1527, 1999
BspANI GGCC 5 cut(s) 192, 225, 542, 801, 997
BspCNI CTCAG 5 cut(s) 69, 1300, 1387, 1506, 2381
BspFNI CGCG 3 cut(s) 266, 408, 668
BspLI GGNNCC 4 cut(s) 639, 1051, 1686, 2212
BspMAI CTGCAG 2 cut(s) 477, 2125
BspMI ACCTGC 1 cut(s) 1213
BspOI GCTAGC 1 cut(s) 1122
BspPI GGATC 3 cut(s) 194, 480, 2309
BspQI GCTCTTC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 2210
BspTI CTTAAG 1 cut(s) 1177
BsrBI CCGCTC 1 cut(s) 771
BsrDI GCAATG 1 cut(s) 639
BsrGI TGTACA 2 cut(s) 1096, 1636
BsrI ACTGG 3 cut(s) 932, 1276, 1719
BssECI CCNNGG 5 cut(s) 778, 986, 1087, 1585, 1803
BssNI GRCGYC 1 cut(s) 1315
BssSI CACGAG 2 cut(s) 436, 678
BssT1I CCWWGG 5 cut(s) 778, 986, 1087, 1585, 1803
Bst2BI CACGAG 2 cut(s) 436, 678
Bst2UI CCWGG 4 cut(s) 227, 515, 695, 1412
Bst4CI ACNGT 5 cut(s) 762, 1129, 1956, 2205, 2210
Bst6I CTCTTC 2 cut(s) 425, 1513
BstACI GRCGYC 1 cut(s) 1315
BstAFI CTTAAG 1 cut(s) 1177
BstAPI GCANNNNNTGC 3 cut(s) 1235, 1608, 1892
BstAUI TGTACA 2 cut(s) 1096, 1636
BstDEI CTNAG 6 cut(s) 77, 1308, 1395, 1425, 1514, 2368
BstDSI CCRYGG 3 cut(s) 778, 986, 1087
BstF5I GGATG 4 cut(s) 113, 447, 969, 2394
BstFNI CGCG 3 cut(s) 266, 408, 668
BstH2I RGCGCY 1 cut(s) 1386
BstHHI GCGC 2 cut(s) 670, 1385
BstMAI GTCTC 3 cut(s) 713, 1322, 2061
BstMWI GCNNNNNNNGC 5 cut(s) 1235, 1391, 1477, 1608, 1892
BstNI CCWGG 4 cut(s) 227, 515, 695, 1412
BstNSI RCATGY 3 cut(s) 632, 1643, 1789
BstSCI CCNGG 5 cut(s) 225, 513, 693, 1052, 1410
BstSFI CTRYAG 4 cut(s) 473, 1332, 1791, 2121
BstSLI GKGCMC 1 cut(s) 1558
BstUI CGCG 3 cut(s) 266, 408, 668
BstV1I GCAGC 3 cut(s) 439, 844, 1480
BstV2I GAAGAC 1 cut(s) 2133
BstX2I RGATCY 2 cut(s) 485, 2036
BstXI CCANNNNNNTGG 1 cut(s) 993
BstYI RGATCY 2 cut(s) 485, 2036
BsuI GTATCC 2 cut(s) 714, 785
BsuRI GGCC 5 cut(s) 192, 225, 542, 801, 997
BtgI CCRYGG 3 cut(s) 778, 986, 1087
BtsCI GGATG 4 cut(s) 113, 447, 969, 2394
BtsI GCAGTG 1 cut(s) 2130
BtsIMutI CAGTG 5 cut(s) 74, 1125, 1712, 2130, 2325
BtuMI TCGCGA 1 cut(s) 266
BveI ACCTGC 1 cut(s) 1213
CfoI GCGC 2 cut(s) 670, 1385
Cfr13I GGNCC 1 cut(s) 800
CseI GACGC 3 cut(s) 596, 1304, 1957
DdeI CTNAG 6 cut(s) 77, 1308, 1395, 1425, 1514, 2368
DraIII CACNNNGTG 2 cut(s) 1129, 2351
EaeI YGGCCR 1 cut(s) 995
Eam1104I CTCTTC 2 cut(s) 425, 1513
EarI CTCTTC 2 cut(s) 425, 1513
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 2194
Eco130I CCWWGG 5 cut(s) 778, 986, 1087, 1585, 1803
Eco24I GRGCYC 1 cut(s) 2196
Eco32I GATATC 2 cut(s) 535, 893
Eco47III AGCGCT 1 cut(s) 1384
Eco53kI GAGCTC 1 cut(s) 2194
Eco57I CTGAAG 3 cut(s) 493, 1174, 2157
Eco88I CYCGRG 1 cut(s) 2080
EcoICRI GAGCTC 1 cut(s) 2194
EcoRI GAATTC 1 cut(s) 1590
EcoRII CCWGG 4 cut(s) 225, 513, 693, 1410
EcoRV GATATC 2 cut(s) 535, 893
EcoT14I CCWWGG 5 cut(s) 778, 986, 1087, 1585, 1803
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 2196
ErhI CCWWGG 5 cut(s) 778, 986, 1087, 1585, 1803
Esp3I CGTCTC 1 cut(s) 1322
FauI CCCGC 3 cut(s) 512, 569, 810
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 4 cut(s) 453, 858, 1469, 1472
FokI GGATG 4 cut(s) 120, 454, 976, 2401
FriOI GRGCYC 1 cut(s) 2196
Fsp4HI GCNGC 4 cut(s) 453, 858, 1469, 1472
FspBI CTAG 6 cut(s) 456, 816, 1119, 1326, 2034, 2159
GlaI GCGC 2 cut(s) 669, 1384
GluI GCNGC 4 cut(s) 453, 858, 1469, 1472
GsuI CTGGAG 2 cut(s) 1293, 2085
HaeII RGCGCY 1 cut(s) 1386
HaeIII GGCC 5 cut(s) 192, 225, 542, 801, 997
HapII CCGG 2 cut(s) 1053, 2412
HgaI GACGC 3 cut(s) 596, 1304, 1957
HhaI GCGC 2 cut(s) 670, 1385
Hin1I GRCGYC 1 cut(s) 1315
Hin6I GCGC 2 cut(s) 668, 1383
HinP1I GCGC 2 cut(s) 668, 1383
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 8 cut(s) 23, 71, 240, 359, 1063, 1452, 2231, 2366
HpaII CCGG 2 cut(s) 1053, 2412
HphI GGTGA 7 cut(s) 161, 334, 592, 935, 1194, 1413, 2147
Hpy188I TCNGA 5 cut(s) 532, 1042, 1193, 1390, 2365
Hpy99I CGWCG 3 cut(s) 271, 1346, 1917
HpyAV CCTTC 6 cut(s) 119, 862, 930, 1698, 2132, 2330
HpyCH4III ACNGT 5 cut(s) 762, 1129, 1956, 2205, 2210
HpyCH4IV ACGT 2 cut(s) 269, 2217
HpyF10VI GCNNNNNNNGC 5 cut(s) 1235, 1391, 1477, 1608, 1892
HpyF3I CTNAG 6 cut(s) 77, 1308, 1395, 1425, 1514, 2368
HpySE526I ACGT 2 cut(s) 269, 2217
Hsp92I GRCGYC 1 cut(s) 1315
HspAI GCGC 2 cut(s) 668, 1383
Ksp22I TGATCA 1 cut(s) 397
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 3 cut(s) 1055, 2199, 2309
Lsp1109I GCAGC 3 cut(s) 439, 844, 1480
LweI GCATC 7 cut(s) 98, 837, 954, 1150, 1381, 1583, 2379
MaeI CTAG 6 cut(s) 456, 816, 1119, 1326, 2034, 2159
MaeII ACGT 2 cut(s) 269, 2217
MaeIII GTNAC 6 cut(s) 72, 599, 621, 923, 2084, 2098
MbiI CCGCTC 1 cut(s) 771
MboII GAAGA 7 cut(s) 96, 442, 587, 1008, 1039, 1530, 2138
MfeI CAATTG 2 cut(s) 246, 1503
MflI RGATCY 2 cut(s) 485, 2036
MhlI GDGCHC 4 cut(s) 303, 437, 1558, 2196
MlsI TGGCCA 1 cut(s) 997
MluNI TGGCCA 1 cut(s) 997
MlyI GAGTC 3 cut(s) 80, 1072, 2360
Mox20I TGGCCA 1 cut(s) 997
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 997
MseI TTAA 7 cut(s) 254, 1178, 1697, 1857, 2028, 2090, 2402
MslI CAYNNNNRTG 3 cut(s) 440, 1086, 1092
Msp20I TGGCCA 1 cut(s) 997
MspCI CTTAAG 1 cut(s) 1177
MspI CCGG 2 cut(s) 1053, 2412
MspR9I CCNGG 5 cut(s) 227, 515, 695, 1054, 1412
MunI CAATTG 2 cut(s) 246, 1503
Mva1269I GAATGC 3 cut(s) 484, 1240, 1613
MvaI CCWGG 4 cut(s) 227, 515, 695, 1412
MvnI CGCG 3 cut(s) 266, 408, 668
MwoI GCNNNNNNNGC 5 cut(s) 1235, 1391, 1477, 1608, 1892
NciI CCSGG 1 cut(s) 1054
NcoI CCATGG 3 cut(s) 778, 986, 1087
NheI GCTAGC 1 cut(s) 1118
NlaIV GGNNCC 4 cut(s) 639, 1051, 1686, 2212
NmuCI GTSAC 3 cut(s) 72, 923, 2098
NruI TCGCGA 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 632, 1643, 1789
PaeI GCATGC 1 cut(s) 632
PaeR7I CTCGAG 1 cut(s) 2080
PaqCI CACCTGC 1 cut(s) 1213
PciI ACATGT 1 cut(s) 1639
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 2214
PctI GAATGC 3 cut(s) 484, 1240, 1613
PfeI GAWTC 5 cut(s) 23, 240, 359, 1452, 2231
PflMI CCANNNNNTGG 1 cut(s) 1004
PfoI TCCNGGA 1 cut(s) 1410
PkrI GCNGC 4 cut(s) 454, 859, 1470, 1473
PleI GAGTC 3 cut(s) 79, 1071, 2360
PpsI GAGTC 3 cut(s) 79, 1071, 2360
PscI ACATGT 1 cut(s) 1639
PsiI TTATAA 3 cut(s) 93, 164, 2267
Psp124BI GAGCTC 1 cut(s) 2196
Psp6I CCWGG 4 cut(s) 225, 513, 693, 1410
PspGI CCWGG 4 cut(s) 225, 513, 693, 1410
PspN4I GGNNCC 4 cut(s) 639, 1051, 1686, 2212
PspPI GGNCC 1 cut(s) 800
PspXI VCTCGAGB 1 cut(s) 2080
PsrI GAACNNNNNNTAC 4 cut(s) 766, 798, 1635, 1667
PstI CTGCAG 2 cut(s) 477, 2125
PsuI RGATCY 2 cut(s) 485, 2036
RruI TCGCGA 1 cut(s) 266
RseI CAYNNNNRTG 3 cut(s) 440, 1086, 1092
SacI GAGCTC 1 cut(s) 2196
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 7 cut(s) 254, 1178, 1697, 1857, 2028, 2090, 2402
SatI GCNGC 4 cut(s) 453, 858, 1469, 1472
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 3 cut(s) 80, 1072, 2360
ScrFI CCNGG 5 cut(s) 227, 515, 695, 1054, 1412
SduI GDGCHC 4 cut(s) 303, 437, 1558, 2196
SfaNI GCATC 7 cut(s) 98, 837, 954, 1150, 1381, 1583, 2379
SfcI CTRYAG 4 cut(s) 473, 1332, 1791, 2121
Sfr274I CTCGAG 1 cut(s) 2080
SlaI CTCGAG 1 cut(s) 2080
SmiMI CAYNNNNRTG 3 cut(s) 440, 1086, 1092
SmlI CTYRAG 3 cut(s) 766, 1177, 2080
SmoI CTYRAG 3 cut(s) 766, 1177, 2080
SphI GCATGC 1 cut(s) 632
SsiI CCGC 9 cut(s) 37, 505, 576, 771, 803, 1092, 1472, 1527, 1999
SspI AATATT 1 cut(s) 2278
SspMI CTAG 6 cut(s) 456, 816, 1119, 1326, 2034, 2159
SstI GAGCTC 1 cut(s) 2196
StyD4I CCNGG 5 cut(s) 225, 513, 693, 1052, 1410
StyI CCWWGG 5 cut(s) 778, 986, 1087, 1585, 1803
TaaI ACNGT 5 cut(s) 762, 1129, 1956, 2205, 2210
TaiI ACGT 2 cut(s) 272, 2220
TatI WGTACW 6 cut(s) 117, 729, 755, 969, 1096, 1636
TauI GCSGC 1 cut(s) 1474
TfiI GAWTC 5 cut(s) 23, 240, 359, 1452, 2231
Tru1I TTAA 7 cut(s) 254, 1178, 1697, 1857, 2028, 2090, 2402
Tru9I TTAA 7 cut(s) 254, 1178, 1697, 1857, 2028, 2090, 2402
TscAI CASTG 5 cut(s) 81, 1132, 1719, 2137, 2332
TseFI GTSAC 3 cut(s) 72, 923, 2098
TseI GCWGC 3 cut(s) 452, 857, 1468
Tsp45I GTSAC 3 cut(s) 72, 923, 2098
TspDTI ATGAA 9 cut(s) 363, 638, 934, 1065, 1275, 1610, 1928, 2031, 2243
TspGWI ACGGA 4 cut(s) 1187, 1961, 2086, 2251
TspRI CASTG 5 cut(s) 81, 1132, 1719, 2137, 2332
Van91I CCANNNNNTGG 1 cut(s) 1004
Vha464I CTTAAG 1 cut(s) 1177
VneI GTGCAC 1 cut(s) 1554
XapI RAATTY 3 cut(s) 1590, 2092, 2112
XbaI TCTAGA 1 cut(s) 2033
XceI RCATGY 3 cut(s) 632, 1643, 1789
XhoI CTCGAG 1 cut(s) 2080
XspI CTAG 6 cut(s) 456, 816, 1119, 1326, 2034, 2159
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.