RLG00000004658

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011770
Physical Location & Seq
Reverse (-)
61217334 .. 61225827
8494 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004658

Sequence Viewer

Length: 2490 bp
ATGACTTTCGCAAAGCTCGATGATTCACCAATGTTCCGCCAGCAGTTGCAAGGCTTGGAAGAAGGGGCAGAGGCATTGAGGGGAAGGTGTTCTAAGTTTTACAAAGGATGTCGAAAGTACACAGAAGGGCTTGGAGAAGCATACGATGGGGAAATTGCTTTTGTAACTGCCCTTGAAACTTTTGGGGGAGGGCATGGTGATCCTATTTTTTCAGCAATGGGGGGCCATATCATGACGAATTTCACAATTGCATTGAGAGAAATCGGAACATACAAGGAAGTTTTGCGGTCACAGGTAGAGCACATGCTAAATGATCGGCTGCTGCAATTTGTAAATGTTGACCTCCAAGATCTCAAGGAAGCCAGAAAGCGTTTCGACAAGGCTTCACTTATATATGATCAGGCACGTGAGAAGTTTCTTTCATTGAGGAAGAGCACTAGGCTGGATATAGCTGCTAGTATAGAGGAGGAACTGAATAATGCAAGATCCTCATTTGAGCAAGCGCGCTTCAACCTGGTTACTGCTCTCTCTGATATTGAGGCCAAAAAGAAGTTTGAGTTTCAGGAAGCTGTTAGTGGGGTGATGGATGCTCACCTTCGTTACTTCAAACAGGGATATGATCTACTGCACACAATGGAACCCTATATTCATCAGGTTTTGGAGTATGCACAACATACGAGAGAAAGTGCCAACCAGGAGCAGATATCGCTTAATGAAAGAATAGTGGAGTACAGAAGGCAGATTGATAGGGAAAGTAGGCAGTCCTTACGTGGTGTTCATGGTTCTTTTAGTGGAGATGGTATGCAGACTTTGGCTAGAAGATCAAATAAACTGATAGAGGAAGTGATGCAATCTGCTGCAAAGGGGAAGATTCAAACCATTCGTCAAGGATATCTCTCAAAACGGTCCTCCAATTTGAGAGGCGACTGGAAAAGAAGGTATTTCATTCTTGATAGTCGAGGGATGCTGTATTACTATCGCAAACCATGGAACCGGTCATCTTCTTCTCCACAGAAGAATAATTACTCCGACTATGGCTCTGGGATTTTGAGTCGATGGCTTTCTTCCCATTATCATGGTGGTGTACATGATGAAAAATCGGTTGCACGTCACACTGTGAACTTGCTGACATCAACAATTAAGGTTGATGCAGACCAGACAGACTTAAGATTCTGCTTCAGGATTATATCACCTTTGAAAATTTACACTTTGCAGGCAGAGAATGCACTCGACCAAATGGATTGGATTGAGAAAATCACTGGAGTAATTTCTTCATTATTGAGTTTCCAGGCACCTGAGAGGCAGCGGCTGTTAGCTAGCTCTATGGGTAGCGACGATCATTTCTCTGCAAGCGAGAGCAATTCATTAGAAAGTTATCCTGATGTTGATCAGACAGCTATTCAGGACCAGTCACCAAAAATCTTCACTAATAATTATTTGCGTACCTATAGAAGTTTGCAACCGCATAAGCCGAGTGTGAAAACTGAGAAGCCAATTGAGATATTGAGAAGAGTACGTGGTAATGAGAAGTGTGCTGATTGTGGGGCACCTGACCCAGATTGGGCATCCTTAAACCTTGGTATTCTTATTTGCATCGAATGTTCTGGTGTTCATCGCAATCTTGGTGTACATGTATCAAAGGTTAGATCACTGACACTTGATGTGAAAGTATGGGAACCTTCTGTCTTAATCTTGTTTCAATCACTGGGGAATACTTATGCAAACTCGATTTGGGAGGAGATGCTGCCTTCAAAAAGTACTTCACAGGCTGATGATAGCATTACTGGTTTTTTCAAGTCTGATAGAAATTCAATTGATCAATCTCTTATGACAAAGCCCTGTTATGATGATCTGATTTCAGTAAAGGAGAGATTTATTCAAGCAAAGTATGCAGAAAAACGTTTTGTTCGCCCCAGAAAGATCAGTCAGAAACTCCACCCTGTGGAGCAACAGTTGTGGAAAAGTGTCTGTACTAATGACAAGCAAGCTGTTTACCGCTATATTGTTAGCTTTGAAGCTGATATTAATGCTGTATGTGGGAAAGCACTATGTGGTATGCCCTTAGAATCTCGAAATGTTACGAAGTGGATGAATAATTCAATAGACAAGCCTTCCTCAAGTAATTCAAATTTATCAAGTAGAAGTGAAGATCGCCTCATAGAAAACATCCCTGAGGGTTGTTCCCTGCTTCACCTAGCCTGCCAAATTGCTGATATTGGCATGGTGGAGCTACTTTTACAGTATGGTGCAAATATAAATGCTTCTGATTCAAAAGGCCAAACGCCACTCCATTATTGTGTTATGAGAGGGAATCCTGCTATTACAAAGATGCTTCTCATGAGGCCTCTCAAGCTGTCCTACCTGTTCAATTTCAGAGGAGCAGATCCTCAAGCCACTGACAGAGAAGGTAAAACACCGTGTAAGCTTGTATCAGAGTCAGCCTTGAATGACAATGGGATGCTTTCAATGCTGACAAACTCAGGCAGATGA

Protein Analysis

830

Amino Acids

94.14

Weight (kDa)

8.65

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 6 - 234 6.7e-37 BAR domain of APPL family
BAR PF03114 64 - 216 4.5e-14 BAR domain
PH PF00169 294 - 423 2.8e-11 PH domain
ArfGap PF01412 498 - 592 4.8e-32 Putative GTPase activating protein for Arf
Ank_2 PF12796 722 - 780 2.5e-10 Ankyrin repeats (3 copies)
Ank PF00023 726 - 756 3.5e-06 Ankyrin repeat
Ank_4 PF13637 730 - 779 2.8e-10 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011770)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61980
fragaria_vesca FvH4_5g09370 FvH4_5g09370 FvH4_5g09370
malus_domestica MD06G1131800.v1.1 MD14G1148100.v1.1
prunus_persica Prupe.5G142800_v2.0.a1
pyrus_communis pycom06g12340 pycom14g12290
rosa_chinensis RchiOBHm_Chr7g0188801
rosa_laevigata RLG00000004658
rosa_multiflora Rmu_sc0003642.1_g000001
rosa_roxburghii Rroxscaffold_3G00266010
rosa_rugosa Rorug06G0494100
rosa_samantha Rh7AG098800 Rh7BG100500 Rh7CG102000 Rh7DG100600
rosa_wichuraiana Rw7G008510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1291, 1546
AccB7I CCANNNNNTGG 1 cut(s) 1942
AccII CGCG 1 cut(s) 505
AciI CCGC 5 cut(s) 37, 286, 1306, 1463, 1996
AclI AACGTT 1 cut(s) 1900
AclWI GGATC 3 cut(s) 194, 480, 2378
AcsI RAATTY 4 cut(s) 238, 1200, 1807, 2128
AcuI CTGAAG 1 cut(s) 1162
AcvI CACGTG 1 cut(s) 407
AdeI CACNNNGTG 3 cut(s) 1117, 1942, 2051
AfaI GTAC 8 cut(s) 119, 731, 1086, 1444, 1515, 1629, 1759, 1972
AfiI CCNNNNNNNGG 2 cut(s) 1561, 1942
AflII CTTAAG 1 cut(s) 1165
AflIII ACRYGT 1 cut(s) 1630
AgeI ACCGGT 1 cut(s) 993
AjiI CACGTC 1 cut(s) 1109
AjnI CCWGG 3 cut(s) 513, 693, 1287
Alw21I GWGCWC 2 cut(s) 303, 437
AlwI GGATC 3 cut(s) 194, 480, 2378
AlwNI CAGNNNCTG 1 cut(s) 1309
AoxI GGCC 4 cut(s) 223, 540, 2275, 2342
ApeKI GCWGC 6 cut(s) 319, 322, 452, 857, 1303, 1744
ApoI RAATTY 4 cut(s) 238, 1200, 1807, 2128
ArsI GACNNNNNNTTYG 2 cut(s) 868, 900
AseI ATTAAT 1 cut(s) 2025
AsiGI ACCGGT 1 cut(s) 993
Asp700I GAANNNNTTC 1 cut(s) 88
AspLEI GCGC 2 cut(s) 505, 507
AspS9I GGNCC 3 cut(s) 223, 906, 1405
AsuHPI GGTGA 7 cut(s) 18, 209, 584, 592, 1182, 1404, 2183
AsuNHI GCTAGC 1 cut(s) 1316
AvaII GGWCC 2 cut(s) 906, 1405
AxyI CCTNAGG 1 cut(s) 2172
BaeGI GKGCMC 1 cut(s) 1549
BanI GGYRCC 2 cut(s) 1291, 1546
BarI GAAGNNNNNNTAC 2 cut(s) 1007, 1039
BbrPI CACGTG 1 cut(s) 407
Bbv12I GWGCWC 2 cut(s) 303, 437
BbvI GCAGC 6 cut(s) 306, 309, 439, 844, 1315, 1731
BccI CCATC 4 cut(s) 140, 577, 791, 1050
BciT130I CCWGG 3 cut(s) 515, 695, 1289
BclI TGATCA 3 cut(s) 397, 1387, 1816
BfaI CTAG 5 cut(s) 438, 456, 816, 1317, 2195
BfmI CTRYAG 1 cut(s) 1447
BfrI CTTAAG 1 cut(s) 1165
BglII AGATCT 1 cut(s) 349
BisI GCNGC 7 cut(s) 320, 323, 453, 858, 1304, 1307, 1745
BlsI GCNGC 7 cut(s) 321, 324, 454, 859, 1305, 1308, 1746
BmcAI AGTACT 1 cut(s) 1759
Bme1390I CCNGG 3 cut(s) 515, 695, 1289
Bme18I GGWCC 2 cut(s) 906, 1405
BmgBI CACGTC 1 cut(s) 1109
BmgT120I GGNCC 3 cut(s) 223, 906, 1405
BmiI GGNNCC 6 cut(s) 224, 639, 992, 1293, 1548, 1677
BmrFI CCNGG 3 cut(s) 515, 695, 1289
BmrI ACTGGG 1 cut(s) 1715
BmsI GCATC 9 cut(s) 577, 837, 954, 1138, 1574, 1602, 1731, 2319, 2448
BmtI GCTAGC 1 cut(s) 1320
BmuI ACTGGG 1 cut(s) 1715
BpmI CTGGAG 1 cut(s) 1281
BpuEI CTTGAG 4 cut(s) 338, 2101, 2333, 2373
BsaAI YACGTR 3 cut(s) 407, 770, 1517
BsaBI GATNNNNATC 1 cut(s) 1386
BsaJI CCNNGG 2 cut(s) 986, 1576
BsaWI WCCGGW 1 cut(s) 993
BsaXI ACNNNNNCTCC 2 cut(s) 1937, 1967
Bsc4I CCNNNNNNNGG 2 cut(s) 1561, 1942
Bse118I RCCGGY 1 cut(s) 993
Bse1I ACTGG 5 cut(s) 932, 1264, 1408, 1710, 1789
Bse21I CCTNAGG 1 cut(s) 2172
Bse3DI GCAATG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 1386
BseBI CCWGG 3 cut(s) 515, 695, 1289
BseDI CCNNGG 2 cut(s) 986, 1576
BseGI GGATG 7 cut(s) 113, 592, 969, 1565, 2094, 2166, 2463
BseJI GATNNNNATC 1 cut(s) 1386
BseLI CCNNNNNNNGG 2 cut(s) 1561, 1942
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 3 cut(s) 1287, 1476, 2163
BseNI ACTGG 5 cut(s) 932, 1264, 1408, 1710, 1789
BsePI GCGCGC 1 cut(s) 503
BseRI GAGGAG 3 cut(s) 479, 1751, 2391
BseSI GKGCMC 1 cut(s) 1549
BseXI GCAGC 6 cut(s) 306, 309, 439, 844, 1315, 1731
BsgI GTGCAG 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 505
BshFI GGCC 4 cut(s) 225, 542, 2277, 2344
BshNI GGYRCC 2 cut(s) 1291, 1546
BshTI ACCGGT 1 cut(s) 993
BsiHKAI GWGCWC 2 cut(s) 303, 437
BsiSI CCGG 1 cut(s) 994
BslI CCNNNNNNNGG 2 cut(s) 1561, 1942
BsmI GAATGC 1 cut(s) 1228
BsnI GGCC 4 cut(s) 225, 542, 2277, 2344
Bsp1286I GDGCHC 3 cut(s) 303, 437, 1549
Bsp1407I TGTACA 2 cut(s) 1084, 1627
Bsp19I CCATGG 1 cut(s) 986
BspACI CCGC 5 cut(s) 37, 286, 1306, 1463, 1996
BspANI GGCC 4 cut(s) 225, 542, 2277, 2344
BspCNI CTCAG 3 cut(s) 1288, 1477, 2164
BspFNI CGCG 1 cut(s) 505
BspHI TCATGA 2 cut(s) 231, 2337
BspLI GGNNCC 6 cut(s) 224, 639, 992, 1293, 1548, 1677
BspOI GCTAGC 1 cut(s) 1320
BspPI GGATC 3 cut(s) 194, 480, 2378
BspQI GCTCTTC 1 cut(s) 425
BspT107I GGYRCC 2 cut(s) 1291, 1546
BspTI CTTAAG 1 cut(s) 1165
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 993
BsrGI TGTACA 2 cut(s) 1084, 1627
BsrI ACTGG 5 cut(s) 932, 1264, 1408, 1710, 1789
BssAI RCCGGY 1 cut(s) 993
BssECI CCNNGG 2 cut(s) 986, 1576
BssHII GCGCGC 1 cut(s) 503
BssT1I CCWWGG 2 cut(s) 986, 1576
Bst2UI CCWGG 3 cut(s) 515, 695, 1289
Bst4CI ACNGT 5 cut(s) 906, 1117, 1953, 2241, 2418
Bst6I CTCTTC 2 cut(s) 425, 1504
BstAFI CTTAAG 1 cut(s) 1165
BstAPI GCANNNNNTGC 2 cut(s) 1223, 1889
BstAUI TGTACA 2 cut(s) 1084, 1627
BstBAI YACGTR 3 cut(s) 407, 770, 1517
BstC8I GCNNGC 8 cut(s) 41, 501, 505, 1215, 1318, 1351, 1986, 2200
BstDEI CTNAG 6 cut(s) 93, 1296, 1485, 2062, 2172, 2479
BstDSI CCRYGG 1 cut(s) 986
BstF5I GGATG 7 cut(s) 113, 592, 969, 1565, 2094, 2166, 2463
BstFNI CGCG 1 cut(s) 505
BstHHI GCGC 2 cut(s) 505, 507
BstMWI GCNNNNNNNGC 5 cut(s) 706, 1223, 1889, 2350, 2467
BstNI CCWGG 3 cut(s) 515, 695, 1289
BstNSI RCATGY 2 cut(s) 307, 1634
BstSCI CCNGG 3 cut(s) 513, 693, 1287
BstSFI CTRYAG 1 cut(s) 1447
BstSLI GKGCMC 1 cut(s) 1549
BstUI CGCG 1 cut(s) 505
BstV1I GCAGC 6 cut(s) 306, 309, 439, 844, 1315, 1731
BstX2I RGATCY 3 cut(s) 349, 485, 2383
BstXI CCANNNNNNTGG 1 cut(s) 1076
BstYI RGATCY 3 cut(s) 349, 485, 2383
Bsu36I CCTNAGG 1 cut(s) 2172
BsuRI GGCC 4 cut(s) 225, 542, 2277, 2344
BtgI CCRYGG 1 cut(s) 986
BtgZI GCGATG 1 cut(s) 1598
BtrI CACGTC 1 cut(s) 1109
BtsCI GGATG 7 cut(s) 113, 592, 969, 1565, 2094, 2166, 2463
BtsIMutI CAGTG 5 cut(s) 1113, 1257, 1649, 1703, 2394
Cac8I GCNNGC 8 cut(s) 41, 501, 505, 1215, 1318, 1351, 1986, 2200
CaiI CAGNNNCTG 1 cut(s) 1309
CciI TCATGA 2 cut(s) 231, 2337
CfoI GCGC 2 cut(s) 505, 507
Cfr10I RCCGGY 1 cut(s) 993
Cfr13I GGNCC 3 cut(s) 223, 906, 1405
CsiI ACCWGGT 1 cut(s) 513
Csp6I GTAC 8 cut(s) 118, 730, 1085, 1443, 1514, 1628, 1758, 1971
CspAI ACCGGT 1 cut(s) 993
CspCI CAANNNNNGTGG 2 cut(s) 1937, 1972
CviQI GTAC 8 cut(s) 118, 730, 1085, 1443, 1514, 1628, 1758, 1971
DdeI CTNAG 6 cut(s) 93, 1296, 1485, 2062, 2172, 2479
DraIII CACNNNGTG 3 cut(s) 1117, 1942, 2051
Eam1104I CTCTTC 2 cut(s) 425, 1504
EarI CTCTTC 2 cut(s) 425, 1504
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 986, 1576
Eco147I AGGCCT 1 cut(s) 2344
Eco32I GATATC 2 cut(s) 705, 893
Eco47I GGWCC 2 cut(s) 906, 1405
Eco57I CTGAAG 1 cut(s) 1162
Eco72I CACGTG 1 cut(s) 407
Eco81I CCTNAGG 1 cut(s) 2172
EcoRII CCWGG 3 cut(s) 513, 693, 1287
EcoRV GATATC 2 cut(s) 705, 893
EcoT14I CCWWGG 2 cut(s) 986, 1576
ErhI CCWWGG 2 cut(s) 986, 1576
FbaI TGATCA 3 cut(s) 397, 1387, 1816
Fnu4HI GCNGC 7 cut(s) 320, 323, 453, 858, 1304, 1307, 1745
FokI GGATG 7 cut(s) 120, 599, 976, 1552, 2101, 2153, 2470
Fsp4HI GCNGC 7 cut(s) 320, 323, 453, 858, 1304, 1307, 1745
FspBI CTAG 5 cut(s) 438, 456, 816, 1317, 2195
GlaI GCGC 2 cut(s) 504, 506
GluI GCNGC 7 cut(s) 320, 323, 453, 858, 1304, 1307, 1745
GsuI CTGGAG 1 cut(s) 1281
HaeIII GGCC 4 cut(s) 225, 542, 2277, 2344
HapII CCGG 1 cut(s) 994
HhaI GCGC 2 cut(s) 505, 507
Hin6I GCGC 2 cut(s) 503, 505
HinP1I GCGC 2 cut(s) 503, 505
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 2423
HinfI GANTC 8 cut(s) 23, 871, 1051, 1170, 2066, 2267, 2311, 2435
HpaII CCGG 1 cut(s) 994
HphI GGTGA 7 cut(s) 18, 209, 584, 592, 1182, 1404, 2183
Hpy166II GTNNAC 6 cut(s) 120, 340, 1085, 1120, 1628, 1993
Hpy188III TCNNGA 8 cut(s) 232, 563, 950, 1180, 1379, 1403, 2070, 2338
Hpy8I GTNNAC 6 cut(s) 120, 340, 1085, 1120, 1628, 1993
Hpy99I CGWCG 1 cut(s) 1337
HpyCH4III ACNGT 5 cut(s) 906, 1117, 1953, 2241, 2418
HpyCH4IV ACGT 5 cut(s) 406, 769, 1108, 1516, 1900
HpyF10VI GCNNNNNNNGC 5 cut(s) 706, 1223, 1889, 2350, 2467
HpyF3I CTNAG 6 cut(s) 93, 1296, 1485, 2062, 2172, 2479
HpySE526I ACGT 5 cut(s) 406, 769, 1108, 1516, 1900
HspAI GCGC 2 cut(s) 503, 505
Ksp22I TGATCA 3 cut(s) 397, 1387, 1816
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 4 cut(s) 697, 1945, 2227, 2378
Lsp1109I GCAGC 6 cut(s) 306, 309, 439, 844, 1315, 1731
LweI GCATC 9 cut(s) 577, 837, 954, 1138, 1574, 1602, 1731, 2319, 2448
MabI ACCWGGT 1 cut(s) 513
MaeI CTAG 5 cut(s) 438, 456, 816, 1317, 2195
MaeII ACGT 5 cut(s) 406, 769, 1108, 1516, 1900
MaeIII GTNAC 7 cut(s) 163, 288, 517, 599, 1109, 1410, 2077
MfeI CAATTG 3 cut(s) 246, 1494, 1812
MflI RGATCY 3 cut(s) 349, 485, 2383
MhlI GDGCHC 3 cut(s) 303, 437, 1549
MlyI GAGTC 2 cut(s) 1060, 2444
MmeI TCCRAC 1 cut(s) 1053
MroXI GAANNNNTTC 1 cut(s) 88
MseI TTAA 6 cut(s) 711, 1140, 1166, 1571, 1688, 2025
MslI CAYNNNNRTG 3 cut(s) 1074, 1080, 2295
MspA1I CMGCKG 1 cut(s) 1306
MspCI CTTAAG 1 cut(s) 1165
MspI CCGG 1 cut(s) 994
MspR9I CCNGG 3 cut(s) 515, 695, 1289
MunI CAATTG 3 cut(s) 246, 1494, 1812
Mva1269I GAATGC 1 cut(s) 1228
MvaI CCWGG 3 cut(s) 515, 695, 1289
MvnI CGCG 1 cut(s) 505
MwoI GCNNNNNNNGC 5 cut(s) 706, 1223, 1889, 2350, 2467
NcoI CCATGG 1 cut(s) 986
NheI GCTAGC 1 cut(s) 1316
NlaIV GGNNCC 6 cut(s) 224, 639, 992, 1293, 1548, 1677
NmeAIII GCCGAG 1 cut(s) 1497
NmuCI GTSAC 3 cut(s) 288, 1109, 1410
NspI RCATGY 2 cut(s) 307, 1634
PagI TCATGA 2 cut(s) 231, 2337
PauI GCGCGC 1 cut(s) 503
PceI AGGCCT 1 cut(s) 2344
PciI ACATGT 1 cut(s) 1630
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 1228
PdmI GAANNNNTTC 1 cut(s) 88
PfeI GAWTC 6 cut(s) 23, 871, 1170, 2066, 2267, 2311
PflMI CCANNNNNTGG 1 cut(s) 1942
PinAI ACCGGT 1 cut(s) 993
PkrI GCNGC 7 cut(s) 321, 324, 454, 859, 1305, 1308, 1746
PleI GAGTC 2 cut(s) 1059, 2443
PmaCI CACGTG 1 cut(s) 407
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 2 cut(s) 1059, 2443
Ppu21I YACGTR 3 cut(s) 407, 770, 1517
PscI ACATGT 1 cut(s) 1630
PshBI ATTAAT 1 cut(s) 2025
Psp1406I AACGTT 1 cut(s) 1900
Psp6I CCWGG 3 cut(s) 513, 693, 1287
PspCI CACGTG 1 cut(s) 407
PspGI CCWGG 3 cut(s) 513, 693, 1287
PspN4I GGNNCC 6 cut(s) 224, 639, 992, 1293, 1548, 1677
PspPI GGNCC 3 cut(s) 223, 906, 1405
PstNI CAGNNNCTG 1 cut(s) 1309
PsuI RGATCY 3 cut(s) 349, 485, 2383
PteI GCGCGC 1 cut(s) 503
RsaI GTAC 8 cut(s) 119, 731, 1086, 1444, 1515, 1629, 1759, 1972
RsaNI GTAC 8 cut(s) 118, 730, 1085, 1443, 1514, 1628, 1758, 1971
RseI CAYNNNNRTG 3 cut(s) 1074, 1080, 2295
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 6 cut(s) 711, 1140, 1166, 1571, 1688, 2025
SatI GCNGC 7 cut(s) 320, 323, 453, 858, 1304, 1307, 1745
Sau96I GGNCC 3 cut(s) 223, 906, 1405
ScaI AGTACT 1 cut(s) 1759
SchI GAGTC 2 cut(s) 1060, 2444
ScrFI CCNGG 3 cut(s) 515, 695, 1289
SduI GDGCHC 3 cut(s) 303, 437, 1549
SexAI ACCWGGT 1 cut(s) 513
SfaNI GCATC 9 cut(s) 577, 837, 954, 1138, 1574, 1602, 1731, 2319, 2448
SfcI CTRYAG 1 cut(s) 1447
SinI GGWCC 2 cut(s) 906, 1405
SmiMI CAYNNNNRTG 3 cut(s) 1074, 1080, 2295
SmlI CTYRAG 5 cut(s) 353, 1165, 2116, 2348, 2388
SmoI CTYRAG 5 cut(s) 353, 1165, 2116, 2348, 2388
SseBI AGGCCT 1 cut(s) 2344
SsiI CCGC 5 cut(s) 37, 286, 1306, 1463, 1996
SspMI CTAG 5 cut(s) 438, 456, 816, 1317, 2195
StuI AGGCCT 1 cut(s) 2344
StyD4I CCNGG 3 cut(s) 513, 693, 1287
StyI CCWWGG 2 cut(s) 986, 1576
TaaI ACNGT 5 cut(s) 906, 1117, 1953, 2241, 2418
TaiI ACGT 5 cut(s) 409, 772, 1111, 1519, 1903
TaqI TCGA 9 cut(s) 18, 112, 375, 958, 1054, 1230, 1596, 1727, 2071
TatI WGTACW 6 cut(s) 117, 729, 1084, 1627, 1757, 1970
TauI GCSGC 1 cut(s) 1309
TfiI GAWTC 6 cut(s) 23, 871, 1170, 2066, 2267, 2311
Tru1I TTAA 6 cut(s) 711, 1140, 1166, 1571, 1688, 2025
Tru9I TTAA 6 cut(s) 711, 1140, 1166, 1571, 1688, 2025
TscAI CASTG 5 cut(s) 1120, 1264, 1656, 1710, 2401
TseFI GTSAC 3 cut(s) 288, 1109, 1410
TseI GCWGC 6 cut(s) 319, 322, 452, 857, 1303, 1744
Tsp45I GTSAC 3 cut(s) 288, 1109, 1410
TspRI CASTG 5 cut(s) 1120, 1264, 1656, 1710, 2401
Van91I CCANNNNNTGG 1 cut(s) 1942
Vha464I CTTAAG 1 cut(s) 1165
VpaK11BI GGWCC 2 cut(s) 906, 1405
VspI ATTAAT 1 cut(s) 2025
XapI RAATTY 4 cut(s) 238, 1200, 1807, 2128
XceI RCATGY 2 cut(s) 307, 1634
XcmI CCANNNNNNNNNTGG 1 cut(s) 1076
XmnI GAANNNNTTC 1 cut(s) 88
XspI CTAG 5 cut(s) 438, 456, 816, 1317, 2195
ZrmI AGTACT 1 cut(s) 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.