MD14G1148100.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011770
Physical Location & Seq
Forward (+)
24089250 .. 24094504
5255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1148100.v1.1.491

Sequence Viewer

Length: 2373 bp
ATGCATTTCGCAAAGCTCGATGATTCGCCTATGTTCCGCCAACAGATACAAGGGTTGGAAGAAAGTGCAGAGTCGCTGAGGGGAAGATGTTATAAGTTTTACAAAGGATGTCGAAAGCATACAGAAGGGCTTGGAGAAGCGTATGATGGTGAAATCGCTTTTATAACTGCCCTCGAAACTTTTGGGGGAGGGCATAATGATCCTATTTTTTCAGATTTAGGAGGCCATGTTATGACCAGATTCACAACTGCATTGAGAGAAATCGCGACGTACAAGGAAGTTTTTCGTTCACAGTTAGAGCACATGCTAAATGATAAGCTGATGCACATTGTCCATGTTGACCTCCATGATGTCAAGGAAGCCCGAAAGCGTTTTGACAAGACTTCACTTGTATATGATCAGGCACGTGAGAAGTTTCTGTCGTTGAGGAAGAGTACGAGGATGGATATAGCTGCTAGTATAGAGGAGGTTACCGTTCTATCTGATATCGAGGCCAAAAAAAGGTTTGAGTTTTCTGAAGCTGTTAGTGGCATGATGGATGCTCATCTCCGTTACTTCAAACACAGTTATGAGTTACTGCATGCAATGGAACCATATATCCATCAGGTTTTGGCTTATGCACAACAGACAAGAGAAAGTTGCAAACAGGAACAGATATCTCTTAATGAAAGGATACTAGAGTACAGGAGGCAGATTGATCGGGAAAGTATGCAGTCCTTGAGCGGTATCCATGGTTCCCCCAGTGGAGATGGTCCACAGCCTCTCTCTCGGAAATCACATAAAGTGATCGAGGAAGTGATGCAATCTGCTGCAAAGGGGAAGGTACAACCCATTCGGCAAGGATATCTATCAAAACGTTCCTCCAATTTGAGAGGTGACTGGAAGCGAAGGTATTTCATCCTTGATAGTCGAGGGATGCTGTACTACTATCGCAAACCATTGACTCGGCCATCTTATGGGAGTCAATCTTCTCCACAGAAGAATAATTACTCTGAAAATGGCTCCGGGATTTTGAGTCGATGGCTTTCATCTCACTACCATGGCGGTGTACATGATGAAAAATCCGTTGCACGCCACACCGTGAACTTGCTGACATCAACGATAAAGGTTGATGCAGACCAGACAGACTTAAGGTTCTGCTTCAGAATTATATCACCAATAAAAATCTACACTTTGCAGGCAGAGAATGCACTTGATCAAATGGATTGGATTGAGAAAATCACTGGAGTAATTGCATCACTATTGAGTTTCCAGACATCTGAGAGGCGTCTCTCAACTAGCCCTACAGGAAGCGACGATCAATTATCTGGAAACGAGGGTAGTTCATTAGAAAGCGCTCCTCATGCTGAGCAGACAGCTATTCTGGAACTCTCACCTAAGATCACAGCTTGTAATTATCTCCGAATGTCTAAAAGTTTGCAGCCGCACAAGTTCAAACCGAAAAGTGAGAAACTGATTGACTTACTGAGAAGAGTGTGTGGTAATGAAAAATGTGCTGATTGTGGTGCACCTGAACCAGATTGGGCATCTTTGAACCTTGGGATTCTTATTTGCATCGAATGTTCTGGTGTCCATCGTAATCTCGGTGTACATGTATCAAAGGTTCGGTCACTGACACTTGATGTGAAAGTATGGGAACCTTCTGTCTTAACCTTGTTTCAATCACTGGGAAATACTTACATAAACTCGATTTGGGAGGAGATGTTGTATTCAAGAAGTTCTTTGCAGGCTGATAACATGCCTATAGGTTTCTCCAGTGCTGATAGAAACGAGCCGTTTCTTATGATAAAGCCATGTTATGACGATCTTATTTCGACAAAGGAGAGGTATATTCATGCAAAGTATGCAGAAAAACTTTTTGTTCGTCATACGAAAAACAATCAGAAGCTCCACGTAACGGGGCAACAGTTGTGCACAAGCGTCCGAGCGAATGACAAGAAAGCAGTTTACCGCTATATTATTGGTTATGAAGCAGATGTTAATGCTAGATCAGTGCAAGCATTAGATGATCATTCTGAAGACAAGCCTTCGTCGAGTAACTTAAATTACGTTTCTAGAAGCGAAATTCAGCCCGCAGAAGACAGCGCTGAGGACTGTTCCCTGCTTCACCTAGCCTGCCAAAGTGCCGATATTGGCATGGTAGAGCTACTTTTACAGTATGGTGCGCACGTAAACGCTACTGATTCAAAAGGTCAAACACCGCTGCATTATTGCGTTATCAGAGGGAACATTGCTATTGCAAAGTTGCTTCTTACGAGGGGAGCTGATCCTCGAGCCACTGACAGAGAAGGTAAAACACCAAGTGAGCTTGTATCAGAATCCGCCTCGAAAGACAATGGGATGTTGGCTCTTTTAACAAACTCCGGCAGATGA

Protein Analysis

791

Amino Acids

89.19

Weight (kDa)

7.85

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 6 - 218 1.3e-26 BAR domain of APPL family
PH PF00169 278 - 411 2.7e-11 PH domain
ArfGap PF01412 485 - 575 8.3e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 697 - 761 7e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 702 - 751 2e-11 Ankyrin repeats (many copies)
Ank_5 PF13857 724 - 769 1.8e-07 Ankyrin repeats (many copies)
Ank PF00023 731 - 762 1.7e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011770)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61980
fragaria_vesca FvH4_5g09370 FvH4_5g09370 FvH4_5g09370
malus_domestica MD06G1131800.v1.1 MD14G1148100.v1.1
prunus_persica Prupe.5G142800_v2.0.a1
pyrus_communis pycom06g12340 pycom14g12290
rosa_chinensis RchiOBHm_Chr7g0188801
rosa_laevigata RLG00000004658
rosa_multiflora Rmu_sc0003642.1_g000001
rosa_roxburghii Rroxscaffold_3G00266010
rosa_rugosa Rorug06G0494100
rosa_samantha Rh7AG098800 Rh7BG100500 Rh7CG102000 Rh7DG100600
rosa_wichuraiana Rw7G008510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 93, 164
Acc16I TGCGCA 1 cut(s) 2166
AccB7I CCANNNNNTGG 1 cut(s) 956
AccBSI CCGCTC 1 cut(s) 723
AccII CGCG 1 cut(s) 266
AciI CCGC 8 cut(s) 37, 723, 1044, 1424, 1951, 2073, 2201, 2322
AclI AACGTT 1 cut(s) 856
AclWI GGATC 2 cut(s) 194, 2261
AcoI YGGCCR 1 cut(s) 947
AcsI RAATTY 1 cut(s) 2064
AcuI CTGAAG 3 cut(s) 537, 1126, 2037
AcvI CACGTG 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 1267
AdeI CACNNNGTG 2 cut(s) 1081, 2303
AfaI GTAC 7 cut(s) 272, 436, 683, 825, 923, 1050, 1590
AfeI AGCGCT 2 cut(s) 1336, 2086
AfiI CCNNNNNNNGG 3 cut(s) 501, 956, 1897
AflII CTTAAG 1 cut(s) 1129
AflIII ACRYGT 1 cut(s) 1591
AgsI TTSAA 6 cut(s) 559, 1435, 1534, 1661, 1713, 2187
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
Alw21I GWGCWC 3 cut(s) 303, 1510, 1916
Alw26I GTCTC 1 cut(s) 1274
Alw44I GTGCAC 2 cut(s) 1506, 1912
AlwI GGATC 2 cut(s) 194, 2261
Ama87I CYCGRG 1 cut(s) 2271
Aor51HI AGCGCT 2 cut(s) 1336, 2086
AoxI GGCC 3 cut(s) 223, 492, 947
ApaLI GTGCAC 2 cut(s) 1506, 1912
ApeKI GCWGC 4 cut(s) 452, 809, 1420, 2203
ApoI RAATTY 1 cut(s) 2064
ArsI GACNNNNNNTTYG 2 cut(s) 1592, 1624
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 3 cut(s) 1337, 2087, 2167
AspS9I GGNCC 1 cut(s) 752
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 5 cut(s) 161, 887, 1146, 1365, 2099
AvaI CYCGRG 1 cut(s) 2271
AvaII GGWCC 1 cut(s) 752
BaeGI GKGCMC 2 cut(s) 1510, 1916
BaeI ACNNNNGTAYC 4 cut(s) 38, 71, 665, 698
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BbrPI CACGTG 1 cut(s) 407
BbsI GAAGAC 2 cut(s) 2025, 2085
Bbv12I GWGCWC 3 cut(s) 303, 1510, 1916
BbvCI CCTCAGC 2 cut(s) 77, 2088
BbvI GCAGC 4 cut(s) 439, 796, 1432, 2190
BccI CCATC 8 cut(s) 140, 436, 529, 609, 743, 958, 1014, 1581
BceAI ACGGC 1 cut(s) 1759
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciVI GTATCC 2 cut(s) 666, 737
BclI TGATCA 3 cut(s) 397, 1195, 2008
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 1 cut(s) 1274
BfaI CTAG 6 cut(s) 456, 677, 1278, 1986, 2055, 2111
BfmI CTRYAG 2 cut(s) 1284, 1743
BfoI RGCGCY 2 cut(s) 1338, 2088
BfrI CTTAAG 1 cut(s) 1129
BfuI GTATCC 2 cut(s) 666, 737
BisI GCNGC 5 cut(s) 453, 810, 1421, 1424, 2204
BlpI GCTNAGC 1 cut(s) 1347
BlsI GCNGC 5 cut(s) 454, 811, 1422, 1425, 2205
Bme1390I CCNGG 1 cut(s) 1006
Bme18I GGWCC 1 cut(s) 752
BmeT110I CYCGRG 1 cut(s) 2271
BmgT120I GGNCC 1 cut(s) 752
BmiI GGNNCC 4 cut(s) 591, 736, 1003, 1638
BmrFI CCNGG 1 cut(s) 1006
BmrI ACTGGG 2 cut(s) 735, 1676
BmsI GCATC 8 cut(s) 312, 529, 789, 906, 1102, 1244, 1535, 1563
BmuI ACTGGG 2 cut(s) 735, 1676
BpiI GAAGAC 2 cut(s) 2025, 2085
BpmI CTGGAG 2 cut(s) 1245, 1738
Bpu10I CCTNAGC 2 cut(s) 77, 2088
Bpu1102I GCTNAGC 1 cut(s) 1347
BpuEI CTTGAG 1 cut(s) 739
BpuMI CCSGG 1 cut(s) 1006
BsaAI YACGTR 3 cut(s) 407, 1894, 2170
BsaBI GATNNNNATC 2 cut(s) 543, 847
BsaHI GRCGYC 1 cut(s) 1267
BsaJI CCNNGG 3 cut(s) 730, 1039, 1537
BsaXI ACNNNNNCTCC 6 cut(s) 213, 243, 1688, 1691, 1718, 1721
Bsc4I CCNNNNNNNGG 3 cut(s) 501, 956, 1897
Bse1I ACTGG 5 cut(s) 741, 884, 1228, 1671, 1755
Bse3DI GCAATG 2 cut(s) 591, 2229
Bse8I GATNNNNATC 2 cut(s) 543, 847
BseDI CCNNGG 3 cut(s) 730, 1039, 1537
BseGI GGATG 6 cut(s) 113, 447, 544, 897, 921, 2346
BseJI GATNNNNATC 2 cut(s) 543, 847
BseLI CCNNNNNNNGG 3 cut(s) 501, 956, 1897
BseMI GCAATG 2 cut(s) 591, 2229
BseMII CTCAG 5 cut(s) 68, 1251, 1338, 1457, 2079
BseNI ACTGG 5 cut(s) 741, 884, 1228, 1671, 1755
BseRI GAGGAG 3 cut(s) 479, 1329, 1712
BseSI GKGCMC 2 cut(s) 1510, 1916
BseXI GCAGC 4 cut(s) 439, 796, 1432, 2190
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 3 cut(s) 225, 494, 949
BsiHKAI GWGCWC 3 cut(s) 303, 1510, 1916
BsiHKCI CYCGRG 1 cut(s) 2271
BsiSI CCGG 2 cut(s) 1005, 2364
BslI CCNNNNNNNGG 3 cut(s) 501, 956, 1897
BsmAI GTCTC 1 cut(s) 1274
BsmBI CGTCTC 1 cut(s) 1274
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 3 cut(s) 225, 494, 949
BsoBI CYCGRG 1 cut(s) 2271
Bsp1286I GDGCHC 3 cut(s) 303, 1510, 1916
Bsp1407I TGTACA 2 cut(s) 1048, 1588
Bsp1720I GCTNAGC 1 cut(s) 1347
Bsp19I CCATGG 2 cut(s) 730, 1039
Bsp68I TCGCGA 1 cut(s) 266
BspACI CCGC 8 cut(s) 37, 723, 1044, 1424, 1951, 2073, 2201, 2322
BspANI GGCC 3 cut(s) 225, 494, 949
BspCNI CTCAG 5 cut(s) 69, 1252, 1339, 1458, 2080
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 4 cut(s) 591, 736, 1003, 1638
BspPI GGATC 2 cut(s) 194, 2261
BspTI CTTAAG 1 cut(s) 1129
BsrBI CCGCTC 1 cut(s) 723
BsrDI GCAATG 2 cut(s) 591, 2229
BsrGI TGTACA 2 cut(s) 1048, 1588
BsrI ACTGG 5 cut(s) 741, 884, 1228, 1671, 1755
BssECI CCNNGG 3 cut(s) 730, 1039, 1537
BssNI GRCGYC 1 cut(s) 1267
BssT1I CCWWGG 3 cut(s) 730, 1039, 1537
Bst4CI ACNGT 7 cut(s) 294, 475, 566, 1081, 1908, 2096, 2157
Bst6I CTCTTC 2 cut(s) 425, 1465
BstACI GRCGYC 1 cut(s) 1267
BstAFI CTTAAG 1 cut(s) 1129
BstAPI GCANNNNNTGC 2 cut(s) 1187, 1844
BstAUI TGTACA 2 cut(s) 1048, 1588
BstBAI YACGTR 3 cut(s) 407, 1894, 2170
BstC8I GCNNGC 7 cut(s) 582, 1072, 1179, 1728, 1998, 2073, 2116
BstDEI CTNAG 6 cut(s) 77, 1260, 1347, 1377, 1466, 2088
BstDSI CCRYGG 2 cut(s) 730, 1039
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 6 cut(s) 113, 447, 544, 897, 921, 2346
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 2 cut(s) 1338, 2088
BstHHI GCGC 3 cut(s) 1337, 2087, 2167
BstMAI GTCTC 1 cut(s) 1274
BstMWI GCNNNNNNNGC 3 cut(s) 1187, 1343, 1844
BstNSI RCATGY 4 cut(s) 307, 584, 1595, 1741
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 1004
BstSFI CTRYAG 2 cut(s) 1284, 1743
BstSLI GKGCMC 2 cut(s) 1510, 1916
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 4 cut(s) 439, 796, 1432, 2190
BstV2I GAAGAC 2 cut(s) 2025, 2085
BsuI GTATCC 2 cut(s) 666, 737
BsuRI GGCC 3 cut(s) 225, 494, 949
BtgI CCRYGG 2 cut(s) 730, 1039
BtsCI GGATG 6 cut(s) 113, 447, 544, 897, 921, 2346
BtsIMutI CAGTG 7 cut(s) 748, 1221, 1610, 1664, 1762, 1998, 2277
BtuMI TCGCGA 1 cut(s) 266
Cac8I GCNNGC 7 cut(s) 582, 1072, 1179, 1728, 1998, 2073, 2116
CfoI GCGC 3 cut(s) 1337, 2087, 2167
Cfr13I GGNCC 1 cut(s) 752
CseI GACGC 2 cut(s) 1256, 1909
Csp6I GTAC 7 cut(s) 271, 435, 682, 824, 922, 1049, 1589
CviQI GTAC 7 cut(s) 271, 435, 682, 824, 922, 1049, 1589
DdeI CTNAG 6 cut(s) 77, 1260, 1347, 1377, 1466, 2088
DraIII CACNNNGTG 2 cut(s) 1081, 2303
EaeI YGGCCR 1 cut(s) 947
Eam1104I CTCTTC 2 cut(s) 425, 1465
EarI CTCTTC 2 cut(s) 425, 1465
EciI GGCGGA 2 cut(s) 26, 2311
Eco130I CCWWGG 3 cut(s) 730, 1039, 1537
Eco32I GATATC 3 cut(s) 487, 657, 845
Eco47I GGWCC 1 cut(s) 752
Eco47III AGCGCT 2 cut(s) 1336, 2086
Eco57I CTGAAG 3 cut(s) 537, 1126, 2037
Eco72I CACGTG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 2271
Eco91I GGTNACC 1 cut(s) 469
EcoO65I GGTNACC 1 cut(s) 469
EcoRV GATATC 3 cut(s) 487, 657, 845
EcoT14I CCWWGG 3 cut(s) 730, 1039, 1537
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 730, 1039, 1537
Esp3I CGTCTC 1 cut(s) 1274
FalI AAGNNNNNCTT 2 cut(s) 1705, 1737
FauI CCCGC 1 cut(s) 2080
FbaI TGATCA 3 cut(s) 397, 1195, 2008
Fnu4HI GCNGC 5 cut(s) 453, 810, 1421, 1424, 2204
FokI GGATG 6 cut(s) 120, 454, 551, 884, 928, 2353
Fsp4HI GCNGC 5 cut(s) 453, 810, 1421, 1424, 2204
FspAI RTGCGCAY 1 cut(s) 2166
FspBI CTAG 6 cut(s) 456, 677, 1278, 1986, 2055, 2111
FspI TGCGCA 1 cut(s) 2166
GlaI GCGC 3 cut(s) 1336, 2086, 2166
GluI GCNGC 5 cut(s) 453, 810, 1421, 1424, 2204
GsuI CTGGAG 2 cut(s) 1245, 1738
HaeII RGCGCY 2 cut(s) 1338, 2088
HaeIII GGCC 3 cut(s) 225, 494, 949
HapII CCGG 2 cut(s) 1005, 2364
HgaI GACGC 2 cut(s) 1256, 1909
HhaI GCGC 3 cut(s) 1337, 2087, 2167
Hin1I GRCGYC 1 cut(s) 1267
Hin6I GCGC 3 cut(s) 1335, 2085, 2165
HinP1I GCGC 3 cut(s) 1335, 2085, 2165
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 9 cut(s) 23, 71, 240, 943, 961, 1015, 1543, 2183, 2318
HpaII CCGG 2 cut(s) 1005, 2364
HphI GGTGA 5 cut(s) 161, 887, 1146, 1365, 2099
Hpy188III TCNNGA 7 cut(s) 265, 701, 1252, 1308, 1364, 1713, 2055
Hpy99I CGWCG 3 cut(s) 271, 1298, 2035
HpyAV CCTTC 6 cut(s) 119, 814, 882, 1650, 2037, 2282
HpyCH4III ACNGT 7 cut(s) 294, 475, 566, 1081, 1908, 2096, 2157
HpyCH4IV ACGT 6 cut(s) 269, 406, 856, 1893, 2049, 2169
HpyF10VI GCNNNNNNNGC 3 cut(s) 1187, 1343, 1844
HpyF3I CTNAG 6 cut(s) 77, 1260, 1347, 1377, 1466, 2088
HpySE526I ACGT 6 cut(s) 269, 406, 856, 1893, 2049, 2169
Hsp92I GRCGYC 1 cut(s) 1267
HspAI GCGC 3 cut(s) 1335, 2085, 2165
Ksp22I TGATCA 3 cut(s) 397, 1195, 2008
LmnI GCTCC 4 cut(s) 1007, 1342, 1893, 2261
Lsp1109I GCAGC 4 cut(s) 439, 796, 1432, 2190
LweI GCATC 8 cut(s) 312, 529, 789, 906, 1102, 1244, 1535, 1563
MaeI CTAG 6 cut(s) 456, 677, 1278, 1986, 2055, 2111
MaeII ACGT 6 cut(s) 269, 406, 856, 1893, 2049, 2169
MaeIII GTNAC 7 cut(s) 469, 551, 573, 875, 1608, 1894, 2036
MbiI CCGCTC 1 cut(s) 723
MboII GAAGA 8 cut(s) 71, 96, 442, 960, 991, 1482, 2030, 2090
MhlI GDGCHC 3 cut(s) 303, 1510, 1916
MluCI AATT 8 cut(s) 865, 985, 1146, 1230, 1301, 1393, 2044, 2064
MlyI GAGTC 4 cut(s) 80, 937, 970, 1024
MmeI TCCRAC 1 cut(s) 36
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 6 cut(s) 663, 1130, 1649, 1980, 2042, 2354
MslI CAYNNNNRTG 3 cut(s) 567, 1038, 1044
MspA1I CMGCKG 1 cut(s) 2203
MspCI CTTAAG 1 cut(s) 1129
MspI CCGG 2 cut(s) 1005, 2364
MspR9I CCNGG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 1192
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 3 cut(s) 1187, 1343, 1844
NciI CCSGG 1 cut(s) 1006
NcoI CCATGG 2 cut(s) 730, 1039
NlaIV GGNNCC 4 cut(s) 591, 736, 1003, 1638
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 2 cut(s) 875, 1608
NruI TCGCGA 1 cut(s) 266
NsbI TGCGCA 1 cut(s) 2166
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 4 cut(s) 307, 584, 1595, 1741
PaeI GCATGC 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 2271
PciI ACATGT 1 cut(s) 1591
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 1192
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 5 cut(s) 23, 240, 1543, 2183, 2318
PflMI CCANNNNNTGG 1 cut(s) 956
PfoI TCCNGGA 1 cut(s) 1004
PkrI GCNGC 5 cut(s) 454, 811, 1422, 1425, 2205
PleI GAGTC 4 cut(s) 79, 937, 969, 1023
PmaCI CACGTG 1 cut(s) 407
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 4 cut(s) 79, 937, 969, 1023
Ppu21I YACGTR 3 cut(s) 407, 1894, 2170
PscI ACATGT 1 cut(s) 1591
PsiI TTATAA 2 cut(s) 93, 164
Psp1406I AACGTT 1 cut(s) 856
PspCI CACGTG 1 cut(s) 407
PspEI GGTNACC 1 cut(s) 469
PspN4I GGNNCC 4 cut(s) 591, 736, 1003, 1638
PspPI GGNCC 1 cut(s) 752
PspXI VCTCGAGB 1 cut(s) 2271
PsrI GAACNNNNNNTAC 4 cut(s) 718, 750, 1587, 1619
RruI TCGCGA 1 cut(s) 266
RsaI GTAC 7 cut(s) 272, 436, 683, 825, 923, 1050, 1590
RsaNI GTAC 7 cut(s) 271, 435, 682, 824, 922, 1049, 1589
RseI CAYNNNNRTG 3 cut(s) 567, 1038, 1044
SaqAI TTAA 6 cut(s) 663, 1130, 1649, 1980, 2042, 2354
SatI GCNGC 5 cut(s) 453, 810, 1421, 1424, 2204
Sau96I GGNCC 1 cut(s) 752
SchI GAGTC 4 cut(s) 80, 937, 970, 1024
ScrFI CCNGG 1 cut(s) 1006
SduI GDGCHC 3 cut(s) 303, 1510, 1916
SfaNI GCATC 8 cut(s) 312, 529, 789, 906, 1102, 1244, 1535, 1563
SfcI CTRYAG 2 cut(s) 1284, 1743
Sfr274I CTCGAG 1 cut(s) 2271
SinI GGWCC 1 cut(s) 752
SlaI CTCGAG 1 cut(s) 2271
SmiMI CAYNNNNRTG 3 cut(s) 567, 1038, 1044
SmlI CTYRAG 3 cut(s) 718, 1129, 2271
SmoI CTYRAG 3 cut(s) 718, 1129, 2271
SphI GCATGC 1 cut(s) 584
Sse9I AATT 8 cut(s) 865, 985, 1146, 1230, 1301, 1393, 2044, 2064
SsiI CCGC 8 cut(s) 37, 723, 1044, 1424, 1951, 2073, 2201, 2322
SspMI CTAG 6 cut(s) 456, 677, 1278, 1986, 2055, 2111
StyD4I CCNGG 1 cut(s) 1004
StyI CCWWGG 3 cut(s) 730, 1039, 1537
TaaI ACNGT 7 cut(s) 294, 475, 566, 1081, 1908, 2096, 2157
TaiI ACGT 6 cut(s) 272, 409, 859, 1896, 2052, 2172
TaqII GACCGA 1 cut(s) 1596
TasI AATT 8 cut(s) 865, 985, 1146, 1230, 1301, 1393, 2044, 2064
TatI WGTACW 4 cut(s) 681, 921, 1048, 1588
TauI GCSGC 1 cut(s) 1426
TfiI GAWTC 5 cut(s) 23, 240, 1543, 2183, 2318
Tru1I TTAA 6 cut(s) 663, 1130, 1649, 1980, 2042, 2354
Tru9I TTAA 6 cut(s) 663, 1130, 1649, 1980, 2042, 2354
TscAI CASTG 7 cut(s) 748, 1228, 1617, 1671, 1762, 1998, 2284
TseFI GTSAC 2 cut(s) 875, 1608
TseI GCWGC 4 cut(s) 452, 809, 1420, 2203
Tsp45I GTSAC 2 cut(s) 875, 1608
TspDTI ATGAA 8 cut(s) 681, 886, 1017, 1071, 1314, 1500, 1823, 1983
TspGWI ACGGA 2 cut(s) 539, 1054
TspRI CASTG 7 cut(s) 748, 1228, 1617, 1671, 1762, 1998, 2284
Van91I CCANNNNNTGG 1 cut(s) 956
Vha464I CTTAAG 1 cut(s) 1129
VneI GTGCAC 2 cut(s) 1506, 1912
VpaK11BI GGWCC 1 cut(s) 752
XapI RAATTY 1 cut(s) 2064
XbaI TCTAGA 1 cut(s) 2054
XceI RCATGY 4 cut(s) 307, 584, 1595, 1741
XhoI CTCGAG 1 cut(s) 2271
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 6 cut(s) 456, 677, 1278, 1986, 2055, 2111
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.