MD06G1142100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014166
Physical Location & Seq
Forward (+)
28580805 .. 28583218
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1142100.v1.1.491

Sequence Viewer

Length: 909 bp
ATGTCGAGCTCCGGTGACCAAACCCTAGCAGCGACACCACCAGCACCACCGCCAGCGGCAACCGAAGAAGAAGACGCAGCGGCGGAACAGAGAGTTTCCGACACCGACAACACTAGCGAGCAGTCCTCCCAGGAATGGGAGACCATGGCCCGAGCTTGGCTCTGCTCGTTTCCCGAGGCCAAAGCTGTCTCCATGGACGAGGTCGAAACCTGGATCGACTCCAACTTCGATTTCATACCCCAAGGCATCAAATCCATGCCCCGGGCCGACCTCTGCCACCGCCTCATTGCCATTCAGAATTGCATGCGACTCCCCAGTCAGGAAGGAAAGGAAGAAGACCAAGGTGATGTTGATATTCCACATGCTCGATTTAAACGCACTGATCAGTGGAGACCGGTTTATATTTGGTTAGAATCGTTGGATAAAGATGACGTGGTTAAGTCCAAAGATATAGCAGACTGGCTAACTGAGAACCAGGATATCCAAGCAGAGTTGTGCTCTAGGCATTCTAAGTATCATTTGATGCACTATATCAAGAAGTGCCATATGAAGATATTGAAGAGGCGGGAAAAGAAGAAGGTATTGGATACTTCTTGTTTTAGCCCAATTACCCTAGAAAATTCCAAGCGTCCGTCTGAAGATGCAGATGCTTCTTCCATATATACCACAGCAGGTATCGTGCAGCCCGACACACCAGCCGCTCTAAAGGTTCACAAAGACGTGCAGATGAAACAAGAAGCTCCAGTGCCATCTCCACTATCTGTATGCAATGCGTTGATCAATGTACCTAAAGATAGTGATGCATATGCGATGAAACGAAAGGAAGCAATTCAGAAATACGAGATATTAGTGGAGCTGGAGAAGCTGCTTGCCCCCATCTTTTTAACGCGCGTAGATACGAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

303

Amino Acids

34.19

Weight (kDa)

5.23

Isoelectric Point (pI)

59.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50410
fragaria_vesca FvH4_5g08300
malus_domestica MD06G1142100.v1.1 MD14G1157400.v1.1
prunus_persica Prupe.5G151300_v2.0.a1 Prupe.5G151300_v2.0.a1
pyrus_communis pycom06g13190
rosa_chinensis RchiOBHm_Chr7g0190221
rosa_laevigata RLG00000004552
rosa_multiflora Rmu_sc0004886.1_g000020
rosa_rugosa Rorug06G0503000 Rorug06G0503000
rosa_samantha Rh7AG108400 Rh7BG111000 Rh7CG113400 Rh7DG111800
rosa_wichuraiana Rw7G009400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 662
AccBSI CCGCTC 1 cut(s) 701
AccII CGCG 2 cut(s) 889, 891
AciI CCGC 7 cut(s) 50, 56, 80, 83, 280, 565, 699
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 1 cut(s) 619
AcuI CTGAAG 1 cut(s) 657
AfaI GTAC 1 cut(s) 786
AfiI CCNNNNNNNGG 5 cut(s) 135, 136, 156, 261, 319
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 1 cut(s) 559
AjiI CACGTC 2 cut(s) 433, 721
AjnI CCWGG 3 cut(s) 129, 209, 474
AjuI GAANNNNNNNTTGG 4 cut(s) 215, 247, 566, 598
AluBI AGCT 6 cut(s) 9, 155, 185, 740, 856, 865
AluI AGCT 6 cut(s) 9, 155, 185, 740, 856, 865
Alw21I GWGCWC 2 cut(s) 11, 500
Alw26I GTCTC 3 cut(s) 134, 193, 385
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 3 cut(s) 150, 173, 261
AoxI GGCC 3 cut(s) 147, 177, 264
ApeKI GCWGC 4 cut(s) 29, 77, 682, 865
ApoI RAATTY 1 cut(s) 619
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AsiGI ACCGGT 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 2 cut(s) 148, 264
AsuC2I CCSGG 2 cut(s) 262, 263
AsuHPI GGTGA 2 cut(s) 26, 356
AvaI CYCGRG 3 cut(s) 150, 173, 261
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 78, 342
Bbv12I GWGCWC 2 cut(s) 11, 500
BbvI GCAGC 4 cut(s) 41, 89, 694, 852
BccI CCATC 2 cut(s) 757, 884
BciT130I CCWGG 3 cut(s) 131, 211, 476
BciVI GTATCC 1 cut(s) 580
BclI TGATCA 2 cut(s) 382, 777
BcnI CCSGG 2 cut(s) 262, 263
BcoDI GTCTC 3 cut(s) 134, 193, 385
BfaI CTAG 4 cut(s) 26, 114, 501, 614
BfuAI ACCTGC 1 cut(s) 662
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 7 cut(s) 30, 57, 78, 81, 683, 699, 866
BlsI GCNGC 7 cut(s) 31, 58, 79, 82, 684, 700, 867
Bme1390I CCNGG 5 cut(s) 131, 211, 262, 263, 476
BmeT110I CYCGRG 3 cut(s) 150, 173, 261
BmgBI CACGTC 2 cut(s) 433, 721
BmgT120I GGNCC 2 cut(s) 148, 264
BmrFI CCNGG 5 cut(s) 131, 211, 262, 263, 476
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 5 cut(s) 255, 513, 631, 637, 790
BmuI ACTGGG 1 cut(s) 309
BpiI GAAGAC 2 cut(s) 78, 342
BplI GAGNNNNNCTC 6 cut(s) 110, 142, 144, 176, 482, 514
BpmI CTGGAG 2 cut(s) 726, 878
BpuMI CCSGG 2 cut(s) 262, 263
BsaI GGTCTC 2 cut(s) 134, 385
BsaJI CCNNGG 8 cut(s) 129, 144, 174, 192, 241, 260, 261, 340
BsaWI WCCGGW 2 cut(s) 11, 394
Bsc4I CCNNNNNNNGG 5 cut(s) 135, 136, 156, 261, 319
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 3 cut(s) 315, 464, 743
Bse3DI GCAATG 2 cut(s) 285, 775
BseBI CCWGG 3 cut(s) 131, 211, 476
BseDI CCNNGG 8 cut(s) 129, 144, 174, 192, 241, 260, 261, 340
BseLI CCNNNNNNNGG 5 cut(s) 135, 136, 156, 261, 319
BseMI GCAATG 2 cut(s) 285, 775
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 3 cut(s) 315, 464, 743
BseXI GCAGC 4 cut(s) 41, 89, 694, 852
BsgI GTGCAG 2 cut(s) 701, 743
Bsh1236I CGCG 2 cut(s) 889, 891
BshFI GGCC 3 cut(s) 149, 179, 266
BshTI ACCGGT 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 11, 500
BsiHKCI CYCGRG 3 cut(s) 150, 173, 261
BsiSI CCGG 3 cut(s) 12, 262, 395
BslI CCNNNNNNNGG 5 cut(s) 135, 136, 156, 261, 319
BsmAI GTCTC 3 cut(s) 134, 193, 385
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 3 cut(s) 149, 179, 266
Bso31I GGTCTC 2 cut(s) 134, 385
BsoBI CYCGRG 3 cut(s) 150, 173, 261
Bsp1286I GDGCHC 2 cut(s) 11, 500
Bsp143I GATC 3 cut(s) 213, 382, 777
Bsp19I CCATGG 2 cut(s) 144, 192
BspACI CCGC 7 cut(s) 50, 56, 80, 83, 280, 565, 699
BspANI GGCC 3 cut(s) 149, 179, 266
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 2 cut(s) 889, 891
BspMI ACCTGC 1 cut(s) 662
BspPI GGATC 1 cut(s) 221
BspTNI GGTCTC 2 cut(s) 134, 385
BsrBI CCGCTC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 285, 775
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 3 cut(s) 315, 464, 743
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 8 cut(s) 129, 144, 174, 192, 241, 260, 261, 340
BssMI GATC 3 cut(s) 213, 382, 777
BssT1I CCWWGG 4 cut(s) 144, 192, 241, 340
Bst2UI CCWGG 3 cut(s) 131, 211, 476
Bst6I CTCTTC 1 cut(s) 554
BstC8I GCNNGC 4 cut(s) 54, 119, 305, 870
BstDEI CTNAG 2 cut(s) 468, 510
BstDSI CCRYGG 2 cut(s) 144, 192
BstEII GGTNACC 1 cut(s) 14
BstFNI CGCG 2 cut(s) 889, 891
BstHHI GCGC 1 cut(s) 891
BstKTI GATC 3 cut(s) 216, 385, 780
BstMAI GTCTC 3 cut(s) 134, 193, 385
BstMBI GATC 3 cut(s) 213, 382, 777
BstMWI GCNNNNNNNGC 1 cut(s) 862
BstNI CCWGG 3 cut(s) 131, 211, 476
BstNSI RCATGY 2 cut(s) 307, 365
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 5 cut(s) 129, 209, 260, 261, 474
BstUI CGCG 2 cut(s) 889, 891
BstV1I GCAGC 4 cut(s) 41, 89, 694, 852
BstV2I GAAGAC 2 cut(s) 78, 342
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 149, 179, 266
BtgI CCRYGG 2 cut(s) 144, 192
BtgZI GCGATG 1 cut(s) 824
BtrI CACGTC 2 cut(s) 433, 721
BtsIMutI CAGTG 3 cut(s) 378, 392, 750
BveI ACCTGC 1 cut(s) 662
Cac8I GCNNGC 4 cut(s) 54, 119, 305, 870
CfoI GCGC 1 cut(s) 891
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 148, 264
Cfr9I CCCGGG 1 cut(s) 261
CseI GACGC 2 cut(s) 83, 617
Csp6I GTAC 1 cut(s) 785
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 5 cut(s) 145, 193, 256, 304, 362
CviQI GTAC 1 cut(s) 785
DdeI CTNAG 2 cut(s) 468, 510
DpnI GATC 3 cut(s) 215, 384, 779
DpnII GATC 3 cut(s) 213, 382, 777
DraI TTTAAA 1 cut(s) 373
DrdI GACNNNNNNGTC 1 cut(s) 315
DseDI GACNNNNNNGTC 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
EciI GGCGGA 1 cut(s) 98
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 4 cut(s) 144, 192, 241, 340
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 2 cut(s) 134, 385
Eco32I GATATC 1 cut(s) 481
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 657
Eco88I CYCGRG 3 cut(s) 150, 173, 261
Eco91I GGTNACC 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 9
EcoO65I GGTNACC 1 cut(s) 14
EcoRII CCWGG 3 cut(s) 129, 209, 474
EcoRV GATATC 1 cut(s) 481
EcoT14I CCWWGG 4 cut(s) 144, 192, 241, 340
EcoT22I ATGCAT 1 cut(s) 805
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 4 cut(s) 144, 192, 241, 340
FaeI CATG 5 cut(s) 148, 196, 259, 307, 365
FatI CATG 5 cut(s) 144, 192, 255, 303, 361
FauI CCCGC 1 cut(s) 558
FauNDI CATATG 2 cut(s) 546, 805
FbaI TGATCA 2 cut(s) 382, 777
Fnu4HI GCNGC 7 cut(s) 30, 57, 78, 81, 683, 699, 866
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 7 cut(s) 30, 57, 78, 81, 683, 699, 866
FspBI CTAG 4 cut(s) 26, 114, 501, 614
GlaI GCGC 1 cut(s) 890
GluI GCNGC 7 cut(s) 30, 57, 78, 81, 683, 699, 866
GsuI CTGGAG 2 cut(s) 726, 878
HaeIII GGCC 3 cut(s) 149, 179, 266
HapII CCGG 3 cut(s) 12, 262, 395
HgaI GACGC 2 cut(s) 83, 617
HhaI GCGC 1 cut(s) 891
Hin1II CATG 5 cut(s) 148, 196, 259, 307, 365
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HinfI GANTC 3 cut(s) 218, 309, 413
HpaII CCGG 3 cut(s) 12, 262, 395
HphI GGTGA 2 cut(s) 26, 356
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 4 cut(s) 100, 297, 637, 834
Hpy188III TCNNGA 3 cut(s) 173, 320, 535
Hpy8I GTNNAC 1 cut(s) 712
HpyAV CCTTC 2 cut(s) 317, 571
HpyCH4IV ACGT 2 cut(s) 432, 720
HpyCH4V TGCA 7 cut(s) 303, 526, 644, 682, 724, 768, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 862
HpyF3I CTNAG 2 cut(s) 468, 510
HpySE526I ACGT 2 cut(s) 432, 720
Hsp92II CATG 5 cut(s) 148, 196, 259, 307, 365
HspAI GCGC 1 cut(s) 889
Ksp22I TGATCA 2 cut(s) 382, 777
Kzo9I GATC 3 cut(s) 213, 382, 777
LmnI GCTCC 3 cut(s) 14, 745, 853
Lsp1109I GCAGC 4 cut(s) 41, 89, 694, 852
LweI GCATC 5 cut(s) 255, 513, 631, 637, 790
MaeI CTAG 4 cut(s) 26, 114, 501, 614
MaeII ACGT 2 cut(s) 432, 720
MaeIII GTNAC 1 cut(s) 14
MalI GATC 3 cut(s) 215, 384, 779
MbiI CCGCTC 1 cut(s) 701
MboI GATC 3 cut(s) 213, 382, 777
MhlI GDGCHC 2 cut(s) 11, 500
MluCI AATT 4 cut(s) 298, 606, 619, 828
MlyI GAGTC 2 cut(s) 212, 303
MmeI TCCRAC 3 cut(s) 123, 246, 399
MnlI CCTC 6 cut(s) 136, 169, 193, 281, 293, 555
Mph1103I ATGCAT 1 cut(s) 805
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 3 cut(s) 372, 438, 884
MspA1I CMGCKG 2 cut(s) 56, 80
MspI CCGG 3 cut(s) 12, 262, 395
MspR9I CCNGG 5 cut(s) 131, 211, 262, 263, 476
Mva1269I GAATGC 1 cut(s) 505
MvaI CCWGG 3 cut(s) 131, 211, 476
MvnI CGCG 2 cut(s) 889, 891
MwoI GCNNNNNNNGC 1 cut(s) 862
NciI CCSGG 2 cut(s) 262, 263
NcoI CCATGG 2 cut(s) 144, 192
NdeI CATATG 2 cut(s) 546, 805
NdeII GATC 3 cut(s) 213, 382, 777
NlaIII CATG 5 cut(s) 148, 196, 259, 307, 365
NmuCI GTSAC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 805
NspI RCATGY 2 cut(s) 307, 365
PaeI GCATGC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PctI GAATGC 1 cut(s) 505
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 200
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 7 cut(s) 31, 58, 79, 82, 684, 700, 867
PleI GAGTC 2 cut(s) 212, 303
PpsI GAGTC 2 cut(s) 212, 303
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 3 cut(s) 129, 209, 474
PspEI GGTNACC 1 cut(s) 14
PspGI CCWGG 3 cut(s) 129, 209, 474
PspPI GGNCC 2 cut(s) 148, 264
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 1 cut(s) 786
RsaNI GTAC 1 cut(s) 785
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 3 cut(s) 372, 438, 884
SatI GCNGC 7 cut(s) 30, 57, 78, 81, 683, 699, 866
Sau3AI GATC 3 cut(s) 213, 382, 777
Sau96I GGNCC 2 cut(s) 148, 264
SchI GAGTC 2 cut(s) 212, 303
ScrFI CCNGG 5 cut(s) 131, 211, 262, 263, 476
SduI GDGCHC 2 cut(s) 11, 500
SfaNI GCATC 5 cut(s) 255, 513, 631, 637, 790
SmaI CCCGGG 1 cut(s) 263
SphI GCATGC 1 cut(s) 307
Sse9I AATT 4 cut(s) 298, 606, 619, 828
SsiI CCGC 7 cut(s) 50, 56, 80, 83, 280, 565, 699
SspMI CTAG 4 cut(s) 26, 114, 501, 614
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 5 cut(s) 129, 209, 260, 261, 474
StyI CCWWGG 4 cut(s) 144, 192, 241, 340
TaiI ACGT 2 cut(s) 435, 723
TaqI TCGA 5 cut(s) 5, 204, 216, 228, 367
TasI AATT 4 cut(s) 298, 606, 619, 828
TauI GCSGC 3 cut(s) 59, 83, 701
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 3 cut(s) 372, 438, 884
Tru9I TTAA 3 cut(s) 372, 438, 884
TscAI CASTG 3 cut(s) 385, 392, 750
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 4 cut(s) 29, 77, 682, 865
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 4 cut(s) 223, 563, 743, 827
TspGWI ACGGA 1 cut(s) 621
TspMI CCCGGG 1 cut(s) 261
TspRI CASTG 3 cut(s) 385, 392, 750
Tth111I GACNNNGTC 1 cut(s) 200
XapI RAATTY 1 cut(s) 619
XceI RCATGY 2 cut(s) 307, 365
XmaI CCCGGG 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 4 cut(s) 26, 114, 501, 614
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.