Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010367

Physical Location & Seq

Genomic Coordinates Forward (+)

28700890 .. 28706307

Gene Structure 5418 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1143400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGGCGGATCCAATCGCAGCCCCGCACAGATCCGCCGCGGACCTCTTCTCGGACCCGCTCGACTCCCACCCTCTCTGGTTCAAGCCCGCGCTCTTCCTCTCGCCAGAGTTCGACTCCGAGTCGTACATCTCCGACCTCCGGACCTTCGTGCCCTTCGACACGCTCCGATCCGAGCTCCAGTGCTACCTGGCCTCCCTCAACCACGAGCTCATCGACCTCATCAACCGCGACTACGCCGACTTCGTCAACCTCAGCACCAAGCTCGTCGACGTCGACTCCGCAGTCGTCCGTATGCGGGCCCCGCTTGTTGAGCTAAGGGAGAAGATCGAGCAGTTCAGAGGCTCCGTGCAGAACTCGCTCGTGGCTTTGACGAACGGATTGAAGCAGAGATCAGAGGCGGCCGAGGCCAGAGAGGTCTTGGAGCTCCTGCTTGATACGTTTCATGTCGTTTCTAAGGTTGAAAAACTAATAAAAGAGCTGCCCAGTGTGCCGGCTGATTGGTCGAACGGAGATGTAAATTTGGCAGAGAAGAATTCTATTAGCAATGGAGCTTCCTTGCAACATACTGAGAACGGAACAAATCTCAGAGACACTCAAAGCATGCTTTTGGAGAGAATTGCTAGTGAAATGAACCGGCTGAAGTTCTATTTCGCTCATGCACAGAACCTGCCTTTCATTGAAAACATGGAGAAGAGGATTCAGGGTGCCAGCCTATTATTAGATGCTAGCTTGGGACATTGTTTTGTAGATGGACTGGAACATCGAGATGCAAATGCAATTTACAATTGTTTACGTGCATATGCTGCAATTGATAACACTAGGGGTGCCGAAGAACTTTTTCGCACAACTATAGTAGCTCCATTGATACAAAAAATTATTCCACATGGAACATCGGGGGCAGCTACAAGACCCTCTGGAGATGACCTTGAGAATGATTATAAGCAAATCAGGACATGTATTGAGAAAGACTGCAAATTTTTGTTGGAAATTTCTTTCGAAGAGAATTCTGGTTTGCACGTTTTTGACTTCTTGGCAAATTCAATTCTCAAAGAGGTTCTCTCAGCGATCCAAAAGGGGAAACCCGGTGCTTTTTCTCCTGGAAGACCAACAGAGTTTTTGAAAAACTACAAATCAAGCCTAAAGTTCTTGGCTCATCTAGAAGGCTATTGTCCATCCAGATCAGCTGTTTCCAAATTTCGAGCGGAAGCTGTTTATATTGACTTCATGAAGCAATGGAACCTTGGGGTTTATTTCTCTTTGAGGTTTCAGGAAATTGCGGGGGCTCTAGATTCTATACTTGTGGAGACCAGTCTTCTCCCTATGCATAATGTACCATCTGGTCAAGGAAATTCTCTAGCTTTAACATTAAAACAAAGTGTCACTTTGTTGGAGTGCTTGGAATCCTGTTGGAGAGAAGATGTCATTGTTCTTTCTTGTGCTGACAAGTTTCTGCGCTTATCATTGCAGCTTCTTTCAAGATACTCAAGTTGGTTGTCGTCTGGACTGGCTGCTCGTAAGATGGGTCAAACCGGCTCCAAATCTGGTTGTGAATGGGCTATTTCAGCTGTTCCTGACGATTTTATATATATTATTCATGACGCAGACCAGCTGTACAAGGTGGTTTGTGGTGACTTCCTTGGGCATGTACTTAAGCTTCTTTCTTCTTGTCCTGCCGATGTTCTTGATCTTGTAAAACAGAGCCTGTTACAAGGAGGAAAATCATTGAATGATTTAGTGCCTCAAGTTATCAACACAATTGTAGAGGCACTAGTTGAAAAATCTGCTGCGGAATTGGCACAGCTAAAGGGAATAACTGCAACATACAGGATGACGAATAAGCCTCTTCCTGTCAGACATTCACCCTATGTGGCAGGAGTATTGCGTCCCCTGAGGGCATTTTTGGAAGGAGAACGAGCTACACAGTATCTTACAAGGGACGCTATCAACGAAGTGCTGCTTAATGCTGCAACCGAGATTACTGGTCGTTATAATGAGCAAGCTGCCAACGTCGTCAGTGTGGCTAGGAAAACGGAGACTTCACTCCAGAGAATAAGGCAAGGTGCACAAAGACGAGGTGGAGCAAGTTCAGATGTTTCGGATCACAATGTATCGGACACTGATAAGATATGCATGCAAATTTTTCTTGACATTCAGGAGTATGGGCGTAACCTCGCCGCCCTCGGTGTTGATGTTTCCAATATTGAGGCGTACCGTTCCTTGTGGGAATGCGTTGCTCCTCCGGACAGGAAAAGCGTGATTGATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005795 GO:0005829 GO:0006810 GO:0006891 GO:0006996 GO:0007030 GO:0008104 GO:0008150 GO:0008565 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016192 GO:0017119 GO:0031984 GO:0032991 GO:0033036 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0048193 GO:0051179 GO:0051234 GO:0071702 GO:0071705 GO:0071840 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COG2 DUF3510

Protein Analysis

756

Amino Acids

83.87

Weight (kDa)

5.59

Isoelectric Point (pI)

39.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COG2_N	PF06148	25 - 99	1e-24	COG2 N-terminal
COG2_C	PF12022	410 - 717	3e-95	COG complex component, COG2, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010367)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24840
fragaria_vesca	FvH4_5g08050 FvH4_5g08050 FvH4_5g08050
malus_domestica	MD06G1143400.v1.1 MD14G1158600.v1.1
prunus_persica	Prupe.5G152600_v2.0.a1
pyrus_communis	pycom06g13310 pycom14g13230
rosa_chinensis	RchiOBHm_Chr7g0190411
rosa_laevigata	RLG00000004535
rosa_multiflora	Rmu_sc0014718.1_g000002 Rmu_sc0017751.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264650
rosa_rugosa	Rorug06G0504500 Rorug06G0504600
rosa_samantha	Rh7AG110300 Rh7BG112800 Rh7CG115100 Rh7DG113400
rosa_wichuraiana	Rw7G009570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	939, 1989
AasI	GACNNNNNNGTC	1 cut(s)	281
AatII	GACGTC	1 cut(s)	273
Acc36I	ACCTGC	1 cut(s)	675
AccB1I	GGYRCC	2 cut(s)	704, 824
AccBSI	CCGCTC	2 cut(s)	58, 1202
AccI	GTMKAC	2 cut(s)	267, 273
AccII	CGCG	3 cut(s)	38, 89, 228
AccIII	TCCGGA	2 cut(s)	138, 2239
AclWI	GGATC	6 cut(s)	2, 15, 24, 162, 1060, 2106
AcoI	YGGCCR	1 cut(s)	399
AcsI	RAATTY	9 cut(s)	517, 532, 974, 987, 1003, 1036, 1193, 1348, 2136
AcuI	CTGAAG	1 cut(s)	659
AcyI	GRCGYC	1 cut(s)	270
AfaI	GTAC	5 cut(s)	125, 1332, 1613, 1647, 2210
AfiI	CCNNNNNNNGG	3 cut(s)	49, 138, 295
AflII	CTTAAG	1 cut(s)	1649
AflIII	ACRYGT	1 cut(s)	953
AgsI	TTSAA	9 cut(s)	82, 382, 461, 680, 1041, 1120, 1476, 1726, 1775
AhdI	GACNNNNNGTC	1 cut(s)	118
AhlI	ACTAGT	1 cut(s)	1768
AjnI	CCWGG	2 cut(s)	186, 1096
Alw21I	GWGCWC	4 cut(s)	177, 210, 426, 2065
Alw26I	GTCTC	3 cut(s)	582, 1298, 2027
Alw44I	GTGCAC	1 cut(s)	2061
AlwI	GGATC	6 cut(s)	2, 15, 24, 162, 1060, 2106
AlwNI	CAGNNNCTG	2 cut(s)	667, 1702
Aor13HI	TCCGGA	2 cut(s)	138, 2239
AoxI	GGCC	4 cut(s)	189, 297, 399, 405
ApaI	GGGCCC	1 cut(s)	301
ApaLI	GTGCAC	1 cut(s)	2061
ApoI	RAATTY	9 cut(s)	517, 532, 974, 987, 1003, 1036, 1193, 1348, 2136
Asp700I	GAANNNNTTC	1 cut(s)	837
AspLEI	GCGC	2 cut(s)	91, 1455
AspS9I	GGNCC	5 cut(s)	40, 52, 141, 297, 298
AsuC2I	CCSGG	1 cut(s)	1083
AsuHPI	GGTGA	2 cut(s)	1640, 1851
AsuII	TTCGAA	1 cut(s)	996
AsuNHI	GCTAGC	1 cut(s)	725
AvaII	GGWCC	3 cut(s)	40, 52, 141
AxyI	CCTNAGG	1 cut(s)	1889
BaeGI	GKGCMC	3 cut(s)	153, 301, 2065
BaeI	ACNNNNGTAYC	2 cut(s)	1471, 1504
BamHI	GGATCC	1 cut(s)	7
BanI	GGYRCC	2 cut(s)	704, 824
BanII	GRGCYC	5 cut(s)	177, 210, 301, 426, 1285
BarI	GAAGNNNNNNTAC	2 cut(s)	1638, 1670
BauI	CACGAG	2 cut(s)	203, 359
BbsI	GAAGAC	2 cut(s)	1108, 1304
Bbv12I	GWGCWC	4 cut(s)	177, 210, 426, 2065
BbvCI	CCTCAGC	1 cut(s)	251
BccI	CCATC	4 cut(s)	743, 1180, 1342, 1513
BcgI	CGANNNNNNTGC	2 cut(s)	244, 278
BciT130I	CCWGG	2 cut(s)	188, 1098
BcnI	CCSGG	1 cut(s)	1083
BcoDI	GTCTC	3 cut(s)	582, 1298, 2027
BcuI	ACTAGT	1 cut(s)	1768
BfaI	CTAG	8 cut(s)	621, 726, 819, 1157, 1286, 1355, 1769, 2022
BfmI	CTRYAG	1 cut(s)	849
BfrI	CTTAAG	1 cut(s)	1649
BfuAI	ACCTGC	1 cut(s)	675
Bme1390I	CCNGG	3 cut(s)	188, 1083, 1098
Bme18I	GGWCC	3 cut(s)	40, 52, 141
BmeRI	GACNNNNNGTC	1 cut(s)	118
BmgT120I	GGNCC	5 cut(s)	40, 52, 141, 297, 298
BmiI	GGNNCC	9 cut(s)	9, 54, 299, 300, 343, 706, 826, 1238, 1534
BmrFI	CCNGG	3 cut(s)	188, 1083, 1098
BmrI	ACTGGG	1 cut(s)	477
BmsI	GCATC	2 cut(s)	712, 757
BmtI	GCTAGC	1 cut(s)	729
BmuI	ACTGGG	1 cut(s)	477
BpiI	GAAGAC	2 cut(s)	1108, 1304
BplI	GAGNNNNNCTC	4 cut(s)	98, 130, 1043, 1075
BpmI	CTGGAG	3 cut(s)	161, 936, 2027
Bpu10I	CCTNAGC	2 cut(s)	251, 314
Bpu14I	TTCGAA	1 cut(s)	996
BpuEI	CTTGAG	3 cut(s)	947, 1468, 1725
BpuMI	CCSGG	1 cut(s)	1083
BsaAI	YACGTR	1 cut(s)	794
BsaHI	GRCGYC	1 cut(s)	270
BsaI	GGTCTC	1 cut(s)	1298
BsaJI	CCNNGG	5 cut(s)	36, 402, 1240, 1636, 2179
BsaWI	WCCGGW	2 cut(s)	138, 2239
BsaXI	ACNNNNNCTCC	4 cut(s)	176, 206, 260, 290
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 138, 295
Bse118I	RCCGGY	3 cut(s)	490, 633, 1529
Bse1I	ACTGG	6 cut(s)	178, 483, 759, 1308, 1509, 1984
Bse21I	CCTNAGG	1 cut(s)	1889
Bse3DI	GCAATG	3 cut(s)	550, 1238, 1460
BseAI	TCCGGA	2 cut(s)	138, 2239
BseBI	CCWGG	2 cut(s)	188, 1098
BseDI	CCNNGG	5 cut(s)	36, 402, 1240, 1636, 2179
BseGI	GGATG	2 cut(s)	1172, 1833
BseLI	CCNNNNNNNGG	3 cut(s)	49, 138, 295
BseMI	GCAATG	3 cut(s)	550, 1238, 1460
BseMII	CTCAG	5 cut(s)	265, 558, 598, 1074, 1880
BseNI	ACTGG	6 cut(s)	178, 483, 759, 1308, 1509, 1984
BseRI	GAGGAG	1 cut(s)	2226
BseSI	GKGCMC	3 cut(s)	153, 301, 2065
BseX3I	CGGCCG	1 cut(s)	399
BsgI	GTGCAG	1 cut(s)	368
Bsh1236I	CGCG	3 cut(s)	38, 89, 228
Bsh1285I	CGRYCG	1 cut(s)	402
BshFI	GGCC	4 cut(s)	191, 299, 401, 407
BshNI	GGYRCC	2 cut(s)	704, 824
BsiEI	CGRYCG	1 cut(s)	402
BsiHKAI	GWGCWC	4 cut(s)	177, 210, 426, 2065
BsiSI	CCGG	6 cut(s)	139, 491, 634, 1083, 1530, 2240
BslFI	GGGAC	3 cut(s)	747, 1869, 1949
BslI	CCNNNNNNNGG	3 cut(s)	49, 138, 295
BsmAI	GTCTC	3 cut(s)	582, 1298, 2027
BsmFI	GGGAC	3 cut(s)	747, 1869, 1949
BsmI	GAATGC	1 cut(s)	2231
BsnI	GGCC	4 cut(s)	191, 299, 401, 407
Bso31I	GGTCTC	1 cut(s)	1298
Bsp119I	TTCGAA	1 cut(s)	996
Bsp120I	GGGCCC	1 cut(s)	297
Bsp1286I	GDGCHC	7 cut(s)	153, 177, 210, 301, 426, 1285, 2065
Bsp13I	TCCGGA	2 cut(s)	138, 2239
Bsp1407I	TGTACA	1 cut(s)	1611
Bsp143I	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
BspANI	GGCC	4 cut(s)	191, 299, 401, 407
BspCNI	CTCAG	5 cut(s)	264, 559, 597, 1073, 1881
BspEI	TCCGGA	2 cut(s)	138, 2239
BspFNI	CGCG	3 cut(s)	38, 89, 228
BspHI	TCATGA	2 cut(s)	1224, 1594
BspLI	GGNNCC	9 cut(s)	9, 54, 299, 300, 343, 706, 826, 1238, 1534
BspMI	ACCTGC	1 cut(s)	675
BspOI	GCTAGC	1 cut(s)	729
BspPI	GGATC	6 cut(s)	2, 15, 24, 162, 1060, 2106
BspQI	GCTCTTC	1 cut(s)	98
BspT104I	TTCGAA	1 cut(s)	996
BspT107I	GGYRCC	2 cut(s)	704, 824
BspTI	CTTAAG	1 cut(s)	1649
BspTNI	GGTCTC	1 cut(s)	1298
BsrBI	CCGCTC	2 cut(s)	58, 1202
BsrDI	GCAATG	3 cut(s)	550, 1238, 1460
BsrFI	RCCGGY	3 cut(s)	490, 633, 1529
BsrGI	TGTACA	1 cut(s)	1611
BsrI	ACTGG	6 cut(s)	178, 483, 759, 1308, 1509, 1984
BssAI	RCCGGY	3 cut(s)	490, 633, 1529
BssECI	CCNNGG	5 cut(s)	36, 402, 1240, 1636, 2179
BssMI	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
BssNI	GRCGYC	1 cut(s)	270
BssSI	CACGAG	2 cut(s)	203, 359
BssT1I	CCWWGG	2 cut(s)	1240, 1636
Bst2BI	CACGAG	2 cut(s)	203, 359
Bst2UI	CCWGG	2 cut(s)	188, 1098
Bst4CI	ACNGT	2 cut(s)	1923, 2213
Bst6I	CTCTTC	5 cut(s)	50, 98, 686, 993, 1848
BstACI	GRCGYC	1 cut(s)	270
BstAFI	CTTAAG	1 cut(s)	1649
BstAPI	GCANNNNNTGC	1 cut(s)	803
BstAUI	TGTACA	1 cut(s)	1611
BstBAI	YACGTR	1 cut(s)	794
BstBI	TTCGAA	1 cut(s)	996
BstC8I	GCNNGC	8 cut(s)	87, 297, 492, 602, 709, 727, 1998, 2132
BstDEI	CTNAG	7 cut(s)	251, 314, 453, 567, 584, 1060, 1889
BstDSI	CCRYGG	1 cut(s)	36
BstF5I	GGATG	2 cut(s)	1172, 1833
BstFNI	CGCG	3 cut(s)	38, 89, 228
BstHHI	GCGC	2 cut(s)	91, 1455
BstKTI	GATC	9 cut(s)	10, 32, 170, 327, 392, 1068, 1181, 1687, 2101
BstMAI	GTCTC	3 cut(s)	582, 1298, 2027
BstMBI	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
BstMCI	CGRYCG	1 cut(s)	402
BstMWI	GCNNNNNNNGC	8 cut(s)	301, 310, 355, 404, 487, 803, 1562, 1793
BstNI	CCWGG	2 cut(s)	188, 1098
BstNSI	RCATGY	4 cut(s)	604, 957, 1646, 2134
BstSCI	CCNGG	3 cut(s)	186, 1081, 1096
BstSFI	CTRYAG	1 cut(s)	849
BstSLI	GKGCMC	3 cut(s)	153, 301, 2065
BstUI	CGCG	3 cut(s)	38, 89, 228
BstV2I	GAAGAC	2 cut(s)	1108, 1304
BstX2I	RGATCY	2 cut(s)	7, 29
BstYI	RGATCY	2 cut(s)	7, 29
BstZI	CGGCCG	1 cut(s)	399
Bsu36I	CCTNAGG	1 cut(s)	1889
BsuRI	GGCC	4 cut(s)	191, 299, 401, 407
BtgI	CCRYGG	1 cut(s)	36
BtsCI	GGATG	2 cut(s)	1172, 1833
BtsIMutI	CAGTG	4 cut(s)	185, 490, 2020, 2115
BveI	ACCTGC	1 cut(s)	675
Cac8I	GCNNGC	8 cut(s)	87, 297, 492, 602, 709, 727, 1998, 2132
CaiI	CAGNNNCTG	2 cut(s)	667, 1702
CciI	TCATGA	2 cut(s)	1224, 1594
CfoI	GCGC	2 cut(s)	91, 1455
Cfr10I	RCCGGY	3 cut(s)	490, 633, 1529
Cfr13I	GGNCC	5 cut(s)	40, 52, 141, 297, 298
Cfr42I	CCGCGG	1 cut(s)	39
CseI	GACGC	3 cut(s)	1607, 1871, 1946
Csp6I	GTAC	5 cut(s)	124, 1331, 1612, 1646, 2209
CviQI	GTAC	5 cut(s)	124, 1331, 1612, 1646, 2209
DdeI	CTNAG	7 cut(s)	251, 314, 453, 567, 584, 1060, 1889
DpnI	GATC	9 cut(s)	9, 31, 169, 326, 391, 1067, 1180, 1686, 2100
DpnII	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
DrdI	GACNNNNNNGTC	1 cut(s)	281
DriI	GACNNNNNGTC	1 cut(s)	118
DseDI	GACNNNNNNGTC	1 cut(s)	281
EaeI	YGGCCR	1 cut(s)	399
EagI	CGGCCG	1 cut(s)	399
Eam1104I	CTCTTC	5 cut(s)	50, 98, 686, 993, 1848
Eam1105I	GACNNNNNGTC	1 cut(s)	118
EarI	CTCTTC	5 cut(s)	50, 98, 686, 993, 1848
EciI	GGCGGA	2 cut(s)	20, 22
Ecl136II	GAGCTC	3 cut(s)	175, 208, 424
EclXI	CGGCCG	1 cut(s)	399
Eco130I	CCWWGG	2 cut(s)	1240, 1636
Eco24I	GRGCYC	5 cut(s)	177, 210, 301, 426, 1285
Eco31I	GGTCTC	1 cut(s)	1298
Eco47I	GGWCC	3 cut(s)	40, 52, 141
Eco52I	CGGCCG	1 cut(s)	399
Eco53kI	GAGCTC	3 cut(s)	175, 208, 424
Eco57I	CTGAAG	1 cut(s)	659
Eco81I	CCTNAGG	1 cut(s)	1889
EcoICRI	GAGCTC	3 cut(s)	175, 208, 424
EcoO109I	RGGNCCY	1 cut(s)	298
EcoRI	GAATTC	2 cut(s)	532, 1003
EcoRII	CCWGG	2 cut(s)	186, 1096
EcoT14I	CCWWGG	2 cut(s)	1240, 1636
EcoT22I	ATGCAT	2 cut(s)	1326, 2132
EcoT38I	GRGCYC	5 cut(s)	177, 210, 301, 426, 1285
ErhI	CCWWGG	2 cut(s)	1240, 1636
FalI	AAGNNNNNCTT	4 cut(s)	1366, 1398, 1941, 1973
FaqI	GGGAC	3 cut(s)	747, 1869, 1949
FauI	CCCGC	6 cut(s)	30, 63, 94, 288, 309, 1270
FauNDI	CATATG	1 cut(s)	799
FblI	GTMKAC	2 cut(s)	267, 273
FokI	GGATG	2 cut(s)	1159, 1840
FriOI	GRGCYC	5 cut(s)	177, 210, 301, 426, 1285
FspBI	CTAG	8 cut(s)	621, 726, 819, 1157, 1286, 1355, 1769, 2022
GlaI	GCGC	2 cut(s)	90, 1454
GsuI	CTGGAG	3 cut(s)	161, 936, 2027
HaeIII	GGCC	4 cut(s)	191, 299, 401, 407
HapII	CCGG	6 cut(s)	139, 491, 634, 1083, 1530, 2240
HgaI	GACGC	3 cut(s)	1607, 1871, 1946
HhaI	GCGC	2 cut(s)	91, 1455
Hin1I	GRCGYC	1 cut(s)	270
Hin6I	GCGC	2 cut(s)	89, 1453
HinP1I	GCGC	2 cut(s)	89, 1453
HincII	GTYRAC	3 cut(s)	247, 268, 274
HindII	GTYRAC	3 cut(s)	247, 268, 274
HindIII	AAGCTT	1 cut(s)	1652
HinfI	GANTC	7 cut(s)	62, 113, 119, 275, 697, 1289, 1400
HpaII	CCGG	6 cut(s)	139, 491, 634, 1083, 1530, 2240
HphI	GGTGA	2 cut(s)	1640, 1851
Hpy166II	GTNNAC	5 cut(s)	247, 268, 274, 791, 2063
Hpy8I	GTNNAC	5 cut(s)	247, 268, 274, 791, 2063
Hpy99I	CGWCG	4 cut(s)	269, 272, 275, 2012
HpyAV	CCTTC	4 cut(s)	154, 163, 1154, 1898
HpyCH4III	ACNGT	2 cut(s)	1923, 2213
HpyCH4IV	ACGT	5 cut(s)	270, 437, 793, 1017, 2007
HpyF10VI	GCNNNNNNNGC	8 cut(s)	301, 310, 355, 404, 487, 803, 1562, 1793
HpyF3I	CTNAG	7 cut(s)	251, 314, 453, 567, 584, 1060, 1889
HpySE526I	ACGT	5 cut(s)	270, 437, 793, 1017, 2007
Hsp92I	GRCGYC	1 cut(s)	270
HspAI	GCGC	2 cut(s)	89, 1453
Kpn2I	TCCGGA	2 cut(s)	138, 2239
KroI	GCCGGC	1 cut(s)	490
KroNI	GCCGGC	1 cut(s)	492
KspI	CCGCGG	1 cut(s)	39
Kzo9I	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
LguI	GCTCTTC	1 cut(s)	98
LweI	GCATC	2 cut(s)	712, 757
MaeI	CTAG	8 cut(s)	621, 726, 819, 1157, 1286, 1355, 1769, 2022
MaeII	ACGT	5 cut(s)	270, 437, 793, 1017, 2007
MaeIII	GTNAC	4 cut(s)	1378, 1628, 1704, 2165
MalI	GATC	9 cut(s)	9, 31, 169, 326, 391, 1067, 1180, 1686, 2100
MbiI	CCGCTC	2 cut(s)	58, 1202
MboI	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
MfeI	CAATTG	3 cut(s)	784, 807, 1755
MflI	RGATCY	2 cut(s)	7, 29
MhlI	GDGCHC	7 cut(s)	153, 177, 210, 301, 426, 1285, 2065
MlyI	GAGTC	4 cut(s)	56, 107, 128, 269
MmeI	TCCRAC	4 cut(s)	156, 963, 1368, 1388
Mph1103I	ATGCAT	2 cut(s)	1326, 2132
MroI	TCCGGA	2 cut(s)	138, 2239
MroNI	GCCGGC	1 cut(s)	490
MroXI	GAANNNNTTC	1 cut(s)	837
MseI	TTAA	4 cut(s)	1361, 1367, 1650, 1959
MslI	CAYNNNNRTG	1 cut(s)	765
MspA1I	CMGCKG	4 cut(s)	38, 1184, 1565, 1609
MspCI	CTTAAG	1 cut(s)	1649
MspI	CCGG	6 cut(s)	139, 491, 634, 1083, 1530, 2240
MspR9I	CCNGG	3 cut(s)	188, 1083, 1098
MunI	CAATTG	3 cut(s)	784, 807, 1755
Mva1269I	GAATGC	1 cut(s)	2231
MvaI	CCWGG	2 cut(s)	188, 1098
MvnI	CGCG	3 cut(s)	38, 89, 228
MwoI	GCNNNNNNNGC	8 cut(s)	301, 310, 355, 404, 487, 803, 1562, 1793
NaeI	GCCGGC	1 cut(s)	492
NciI	CCSGG	1 cut(s)	1083
NdeI	CATATG	1 cut(s)	799
NdeII	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
NgoMIV	GCCGGC	1 cut(s)	490
NheI	GCTAGC	1 cut(s)	725
NlaIV	GGNNCC	9 cut(s)	9, 54, 299, 300, 343, 706, 826, 1238, 1534
NmeAIII	GCCGAG	1 cut(s)	427
NmuCI	GTSAC	2 cut(s)	1378, 1628
NsiI	ATGCAT	2 cut(s)	1326, 2132
NspI	RCATGY	4 cut(s)	604, 957, 1646, 2134
NspV	TTCGAA	1 cut(s)	996
PaeI	GCATGC	2 cut(s)	604, 2134
PagI	TCATGA	2 cut(s)	1224, 1594
PciI	ACATGT	1 cut(s)	953
PciSI	GCTCTTC	1 cut(s)	98
PcsI	WCGNNNNNNNCGW	5 cut(s)	153, 210, 240, 270, 1944
PctI	GAATGC	1 cut(s)	2231
PdiI	GCCGGC	1 cut(s)	492
PdmI	GAANNNNTTC	1 cut(s)	837
PfeI	GAWTC	3 cut(s)	697, 1289, 1400
PflFI	GACNNNGTC	1 cut(s)	242
PfoI	TCCNGGA	1 cut(s)	1096
PleI	GAGTC	4 cut(s)	56, 107, 127, 269
PpsI	GAGTC	4 cut(s)	56, 107, 127, 269
Ppu21I	YACGTR	1 cut(s)	794
PscI	ACATGT	1 cut(s)	953
PsiI	TTATAA	2 cut(s)	939, 1989
Psp124BI	GAGCTC	3 cut(s)	177, 210, 426
Psp6I	CCWGG	2 cut(s)	186, 1096
PspGI	CCWGG	2 cut(s)	186, 1096
PspN4I	GGNNCC	9 cut(s)	9, 54, 299, 300, 343, 706, 826, 1238, 1534
PspOMI	GGGCCC	1 cut(s)	297
PspPI	GGNCC	5 cut(s)	40, 52, 141, 297, 298
PstNI	CAGNNNCTG	2 cut(s)	667, 1702
PsuI	RGATCY	2 cut(s)	7, 29
PsyI	GACNNNGTC	1 cut(s)	242
PvuII	CAGCTG	3 cut(s)	1184, 1565, 1609
RsaI	GTAC	5 cut(s)	125, 1332, 1613, 1647, 2210
RsaNI	GTAC	5 cut(s)	124, 1331, 1612, 1646, 2209
RseI	CAYNNNNRTG	1 cut(s)	765
SacI	GAGCTC	3 cut(s)	177, 210, 426
SacII	CCGCGG	1 cut(s)	39
SalI	GTCGAC	2 cut(s)	266, 272
SapI	GCTCTTC	1 cut(s)	98
SaqAI	TTAA	4 cut(s)	1361, 1367, 1650, 1959
Sau3AI	GATC	9 cut(s)	7, 29, 167, 324, 389, 1065, 1178, 1684, 2098
Sau96I	GGNCC	5 cut(s)	40, 52, 141, 297, 298
SchI	GAGTC	4 cut(s)	56, 107, 128, 269
ScrFI	CCNGG	3 cut(s)	188, 1083, 1098
SduI	GDGCHC	7 cut(s)	153, 177, 210, 301, 426, 1285, 2065
SfaNI	GCATC	2 cut(s)	712, 757
SfcI	CTRYAG	1 cut(s)	849
Sfr303I	CCGCGG	1 cut(s)	39
SfuI	TTCGAA	1 cut(s)	996
SgrBI	CCGCGG	1 cut(s)	39
SgrDI	CGTCGACG	1 cut(s)	266
SinI	GGWCC	3 cut(s)	40, 52, 141
SmiMI	CAYNNNNRTG	1 cut(s)	765
SmlI	CTYRAG	4 cut(s)	926, 1483, 1649, 1740
SmoI	CTYRAG	4 cut(s)	926, 1483, 1649, 1740
SpeI	ACTAGT	1 cut(s)	1768
SphI	GCATGC	2 cut(s)	604, 2134
SspI	AATATT	1 cut(s)	2200
SspMI	CTAG	8 cut(s)	621, 726, 819, 1157, 1286, 1355, 1769, 2022
SstI	GAGCTC	3 cut(s)	177, 210, 426
StyD4I	CCNGG	3 cut(s)	186, 1081, 1096
StyI	CCWWGG	2 cut(s)	1240, 1636
TaaI	ACNGT	2 cut(s)	1923, 2213
TaiI	ACGT	5 cut(s)	273, 440, 796, 1020, 2010
TatI	WGTACW	2 cut(s)	1611, 1645
TauI	GCSGC	3 cut(s)	38, 401, 2177
TfiI	GAWTC	3 cut(s)	697, 1289, 1400
Tru1I	TTAA	4 cut(s)	1361, 1367, 1650, 1959
Tru9I	TTAA	4 cut(s)	1361, 1367, 1650, 1959
TscAI	CASTG	4 cut(s)	185, 490, 2020, 2122
TseFI	GTSAC	2 cut(s)	1378, 1628
Tsp45I	GTSAC	2 cut(s)	1378, 1628
TspDTI	ATGAA	6 cut(s)	431, 644, 664, 1213, 1241, 1583
TspGWI	ACGGA	6 cut(s)	278, 334, 390, 522, 588, 2045
TspRI	CASTG	4 cut(s)	185, 490, 2020, 2122
Tth111I	GACNNNGTC	1 cut(s)	242
Vha464I	CTTAAG	1 cut(s)	1649
VneI	GTGCAC	1 cut(s)	2061
VpaK11BI	GGWCC	3 cut(s)	40, 52, 141
XapI	RAATTY	9 cut(s)	517, 532, 974, 987, 1003, 1036, 1193, 1348, 2136
XbaI	TCTAGA	2 cut(s)	1156, 1285
XceI	RCATGY	4 cut(s)	604, 957, 1646, 2134
XcmI	CCANNNNNNNNNTGG	1 cut(s)	415
XmiI	GTMKAC	2 cut(s)	267, 273
XmnI	GAANNNNTTC	1 cut(s)	837
XspI	CTAG	8 cut(s)	621, 726, 819, 1157, 1286, 1355, 1769, 2022
ZraI	GACGTC	1 cut(s)	271
Zsp2I	ATGCAT	2 cut(s)	1326, 2132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.