Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004712

Physical Location & Seq

Genomic Coordinates Forward (+)

28740691 .. 28748484

Gene Structure 7794 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1144000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGTCCGCCTGCTCCGCCGCCAACCTCACTTTTCACGATCCCGAAAGAGAATCATATCCATACCATGAGACAGAAGTCTCCGGATCCACGAAGGAAAGAGGGTTGCGTGGCAAATTGAATAAGGTTGTCCTGGCCTATAGTGGTGGCTTAGACACATCAGTCATTGTTCCGTGGTTAAGGGAGAACTATGGTTGTGATGTTGTTTGCTTCACTGCCGATGTTGGTCAAGGTATAAAAGAATTGGATGGTCTGGAAAAGAAGGCCAAGGCCAGTGGGGCTTCTCAGTTAGTAGTGCAGGATCTAAAGGAGGAGTTTGTAAAGGACTACATATTCCCTTGCTTGCGGGCAGGTGCAATATATGAGAGGAAATACTTGCTGGGGACTTCAATGGCCCGCCCTCTTATTGCTAAGGCCATGGTTGATGTTGCAAAAGAAGTTGGAGCTGATGCTGTAGCTCATGGATGCACCGGGAAAGGAAATGATCAGGCATGCCTTCTAATTCAAAGCGTTCGCTTTGAGCTGACATTTTTCGCTCTCAATCCCAAACTAAATGTTGTGGCTCCTTGGAGGGAGTGGGATATTACAGGCAGAGAAGATGCTATTGAATACGCTAAGAAACATAATGTGCCTGTCCCAGTGACAAAGAAATCCATATACAGCAGAGACGGCAATTTATGGCACCTTAGTCATGAGGGTGACATTTTGGAGGACCCGGAGAATGAGCCTAAGAAGGATATGTTCATGTTAACAGTCGATCCAGAAGATGCACCAAATGAACCTGAGTATGTAGAAATCGGAATTGTTTCAGGAATTCCGGTTTCGGTCAATGGGAAAGAGCTTTCACCGGCCTCTCTACTTGATCATCTAAATCAGATTGGTGGAAAACACGGAATTGGCCGTGTGGACATGGTTGAAAACCGGCTAGTTGGCATGAAGAGCCGTGGAGTCTATGAAACTCCTGGAGGCACCATCCTCTTCACTGCTGCACGAGAGCTTGAGTCTTTAACACTGGACCGAGAAACAATGCAAGTTAAAGACTCATTAGCCCTCAAGTACGCGGAGCTGGTGTACGCAGGCAGGTGGTTCGACCCACTTCGCGAGTCAATGGATTCGTTCATGAAGGAAATCTCTAAAACAACCACCGGATCAGTAACTCTGAAGCTGTACAAGGGTTCGGTTACTGTGACGAGCCGGAAGAGCCCATACAGCCTGTACAGGCAGGACATCGCATCGTTCGAGAGCGGGGATATATATGATCAAGCTGATGCTGCTGGGTTCATTAGGCTGTATGGCCTTCCCATTAGGGTTCGAGCAATGCTCGACGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0000053 GO:0003674 GO:0003824 GO:0004055 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006575 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0019627 GO:0019752 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0071941 GO:0072350 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko01100 ko01110 ko01130 ko01230 ko05418 map00220 map00250 map01100 map01110 map01130 map01230 map05418

Pfam Domains

Protein Families

Arginosuc_synth

Protein Analysis

443

Amino Acids

48.87

Weight (kDa)

5.48

Isoelectric Point (pI)

33.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arginosuc_synth	PF00764	41 - 210	7.6e-65	Arginosuccinate synthase N-terminal HUP domain
Arginosuc_syn_C	PF20979	219 - 436	1.2e-82	Arginosuccinate synthase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24830 AT4G24830
fragaria_vesca	FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g30030
malus_domestica	MD06G1144000.v1.1 MD06G1144100.v1.1 MD14G1159300.v1.1
prunus_persica	Prupe.5G153100_v2.0.a1 Prupe.5G153100_v2.0.a1 Prupe.7G058500_v2.0.a1
pyrus_communis	pycom06g13360 pycom14g13300
rosa_chinensis	RchiOBHm_Chr7g0190471 RchiOBHm_Chr7g0190481
rosa_laevigata	RLG00000004524 RLG00000004526
rosa_multiflora	Rmu_sc0000526.1_g000010
rosa_roxburghii	Rroxscaffold_3G00264570
rosa_rugosa	Rorug06G0504800
rosa_samantha	Rh7AG110900 Rh7BG113700 Rh7BG113800 Rh7CG115900 Rh7DG114600 Rh7DG114700
rosa_wichuraiana	Rw0G023270 Rw7G009670 Rw7G009680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	338, 1068
AasI	GACNNNNNNGTC	1 cut(s)	158
Acc36I	ACCTGC	2 cut(s)	338, 1068
AccB1I	GGYRCC	2 cut(s)	678, 965
AccBSI	CCGCTC	1 cut(s)	1242
AccII	CGCG	2 cut(s)	1058, 1098
AccIII	TCCGGA	1 cut(s)	80
AciI	CCGC	7 cut(s)	6, 15, 18, 343, 394, 1058, 1242
AclWI	GGATC	6 cut(s)	32, 78, 91, 306, 749, 1153
AcoI	YGGCCR	1 cut(s)	895
AcsI	RAATTY	1 cut(s)	810
AcuI	CTGAAG	1 cut(s)	1178
AfaI	GTAC	4 cut(s)	1055, 1070, 1166, 1214
AgsI	TTSAA	5 cut(s)	118, 387, 503, 605, 914
AjnI	CCWGG	2 cut(s)	129, 958
AluBI	AGCT	8 cut(s)	443, 455, 520, 838, 994, 1063, 1162, 1262
AluI	AGCT	8 cut(s)	443, 455, 520, 838, 994, 1063, 1162, 1262
Alw26I	GTCTC	3 cut(s)	62, 82, 657
AlwI	GGATC	6 cut(s)	32, 78, 91, 306, 749, 1153
Aor13HI	TCCGGA	1 cut(s)	80
AoxI	GGCC	8 cut(s)	132, 261, 267, 390, 411, 846, 895, 1291
ApeKI	GCWGC	2 cut(s)	983, 1268
ApoI	RAATTY	1 cut(s)	810
Asp700I	GAANNNNTTC	1 cut(s)	802
AspS9I	GGNCC	3 cut(s)	391, 709, 1012
AsuC2I	CCSGG	2 cut(s)	469, 713
AsuHPI	GGTGA	2 cut(s)	707, 834
AvaII	GGWCC	2 cut(s)	709, 1012
BamHI	GGATCC	1 cut(s)	83
BanI	GGYRCC	2 cut(s)	678, 965
BanII	GRGCYC	1 cut(s)	1202
BarI	GAAGNNNNNNTAC	2 cut(s)	1187, 1219
BauI	CACGAG	1 cut(s)	987
BbvI	GCAGC	2 cut(s)	970, 1255
BccI	CCATC	2 cut(s)	239, 977
BceAI	ACGGC	3 cut(s)	682, 882, 924
BcgI	CGANNNNNNTGC	2 cut(s)	1066, 1100
BciT130I	CCWGG	2 cut(s)	131, 960
BclI	TGATCA	3 cut(s)	481, 859, 1255
BcnI	CCSGG	2 cut(s)	469, 713
BcoDI	GTCTC	3 cut(s)	62, 82, 657
BfaI	CTAG	1 cut(s)	923
BfmI	CTRYAG	2 cut(s)	136, 450
BfuAI	ACCTGC	2 cut(s)	338, 1068
BglI	GCCNNNNNGGC	1 cut(s)	275
BisI	GCNGC	3 cut(s)	18, 984, 1269
BlsI	GCNGC	3 cut(s)	19, 985, 1270
Bme1390I	CCNGG	4 cut(s)	131, 469, 713, 960
Bme18I	GGWCC	2 cut(s)	709, 1012
BmgT120I	GGNCC	3 cut(s)	391, 709, 1012
BmiI	GGNNCC	5 cut(s)	85, 561, 680, 711, 967
BmrFI	CCNGG	4 cut(s)	131, 469, 713, 960
BmrI	ACTGGG	1 cut(s)	629
BmsI	GCATC	6 cut(s)	436, 452, 586, 754, 1238, 1255
BmuI	ACTGGG	1 cut(s)	629
BoxI	GACNNNNGTC	1 cut(s)	74
BplI	GAGNNNNNCTC	1 cut(s)	1302
BpmI	CTGGAG	1 cut(s)	981
Bpu10I	CCTNAGC	1 cut(s)	408
BpuEI	CTTGAG	2 cut(s)	1016, 1034
BpuMI	CCSGG	2 cut(s)	469, 713
BsaJI	CCNNGG	5 cut(s)	170, 264, 414, 563, 940
BsaWI	WCCGGW	3 cut(s)	80, 814, 1142
BsaXI	ACNNNNNCTCC	2 cut(s)	1052, 1082
Bse118I	RCCGGY	2 cut(s)	844, 918
Bse1I	ACTGG	3 cut(s)	270, 635, 1014
Bse3DI	GCAATG	1 cut(s)	1320
BseAI	TCCGGA	1 cut(s)	80
BseBI	CCWGG	2 cut(s)	131, 960
BseDI	CCNNGG	5 cut(s)	170, 264, 414, 563, 940
BseGI	GGATG	3 cut(s)	250, 467, 969
BseMI	GCAATG	1 cut(s)	1320
BseMII	CTCAG	2 cut(s)	296, 771
BseNI	ACTGG	3 cut(s)	270, 635, 1014
BseRI	GAGGAG	1 cut(s)	323
BseXI	GCAGC	2 cut(s)	970, 1255
BseYI	CCCAGC	2 cut(s)	376, 1271
BsgI	GTGCAG	2 cut(s)	314, 969
Bsh1236I	CGCG	2 cut(s)	1058, 1098
BshFI	GGCC	8 cut(s)	134, 263, 269, 392, 413, 848, 897, 1293
BshNI	GGYRCC	2 cut(s)	678, 965
BsiSI	CCGG	8 cut(s)	81, 468, 713, 815, 845, 919, 1143, 1192
BslFI	GGGAC	2 cut(s)	394, 617
BsmAI	GTCTC	3 cut(s)	62, 82, 657
BsmBI	CGTCTC	1 cut(s)	657
BsmFI	GGGAC	2 cut(s)	394, 617
BsnI	GGCC	8 cut(s)	134, 263, 269, 392, 413, 848, 897, 1293
Bsp1286I	GDGCHC	1 cut(s)	1202
Bsp13I	TCCGGA	1 cut(s)	80
Bsp1407I	TGTACA	2 cut(s)	1164, 1212
Bsp143I	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
Bsp19I	CCATGG	1 cut(s)	414
Bsp68I	TCGCGA	1 cut(s)	1098
BspACI	CCGC	7 cut(s)	6, 15, 18, 343, 394, 1058, 1242
BspANI	GGCC	8 cut(s)	134, 263, 269, 392, 413, 848, 897, 1293
BspCNI	CTCAG	2 cut(s)	295, 772
BspEI	TCCGGA	1 cut(s)	80
BspFNI	CGCG	2 cut(s)	1058, 1098
BspHI	TCATGA	2 cut(s)	688, 1116
BspLI	GGNNCC	5 cut(s)	85, 561, 680, 711, 967
BspMI	ACCTGC	2 cut(s)	338, 1068
BspPI	GGATC	6 cut(s)	32, 78, 91, 306, 749, 1153
BspQI	GCTCTTC	2 cut(s)	929, 1190
BspT107I	GGYRCC	2 cut(s)	678, 965
BsrBI	CCGCTC	1 cut(s)	1242
BsrDI	GCAATG	1 cut(s)	1320
BsrFI	RCCGGY	2 cut(s)	844, 918
BsrGI	TGTACA	2 cut(s)	1164, 1212
BsrI	ACTGG	3 cut(s)	270, 635, 1014
BssAI	RCCGGY	2 cut(s)	844, 918
BssECI	CCNNGG	5 cut(s)	170, 264, 414, 563, 940
BssMI	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
BssSI	CACGAG	1 cut(s)	987
BssT1I	CCWWGG	3 cut(s)	264, 414, 563
Bst2BI	CACGAG	1 cut(s)	987
Bst2UI	CCWGG	2 cut(s)	131, 960
Bst4CI	ACNGT	2 cut(s)	751, 1183
Bst6I	CTCTTC	3 cut(s)	929, 980, 1190
BstAUI	TGTACA	2 cut(s)	1164, 1212
BstC8I	GCNNGC	6 cut(s)	10, 341, 345, 394, 490, 1075
BstDEI	CTNAG	7 cut(s)	148, 282, 408, 612, 683, 726, 780
BstDSI	CCRYGG	3 cut(s)	170, 414, 940
BstF5I	GGATG	3 cut(s)	250, 467, 969
BstFNI	CGCG	2 cut(s)	1058, 1098
BstKTI	GATC	8 cut(s)	40, 86, 301, 484, 757, 862, 1148, 1258
BstMAI	GTCTC	3 cut(s)	62, 82, 657
BstMBI	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 275, 666, 936, 1197, 1206, 1268
BstNI	CCWGG	2 cut(s)	131, 960
BstNSI	RCATGY	1 cut(s)	492
BstPAI	GACNNNNGTC	1 cut(s)	74
BstSCI	CCNGG	4 cut(s)	129, 467, 711, 958
BstSFI	CTRYAG	2 cut(s)	136, 450
BstUI	CGCG	2 cut(s)	1058, 1098
BstV1I	GCAGC	2 cut(s)	970, 1255
BstX2I	RGATCY	2 cut(s)	83, 298
BstYI	RGATCY	2 cut(s)	83, 298
BsuRI	GGCC	8 cut(s)	134, 263, 269, 392, 413, 848, 897, 1293
BtgI	CCRYGG	3 cut(s)	170, 414, 940
BtgZI	GCGATG	1 cut(s)	1210
BtsCI	GGATG	3 cut(s)	250, 467, 969
BtsI	GCAGTG	2 cut(s)	210, 978
BtsIMutI	CAGTG	5 cut(s)	210, 277, 642, 978, 1007
BtuMI	TCGCGA	1 cut(s)	1098
BveI	ACCTGC	2 cut(s)	338, 1068
Cac8I	GCNNGC	6 cut(s)	10, 341, 345, 394, 490, 1075
CciI	TCATGA	2 cut(s)	688, 1116
Cfr10I	RCCGGY	2 cut(s)	844, 918
Cfr13I	GGNCC	3 cut(s)	391, 709, 1012
Csp6I	GTAC	4 cut(s)	1054, 1069, 1165, 1213
CspCI	CAANNNNNGTGG	2 cut(s)	253, 288
CviAII	CATG	9 cut(s)	65, 415, 458, 489, 689, 742, 907, 931, 1117
CviQI	GTAC	4 cut(s)	1054, 1069, 1165, 1213
DdeI	CTNAG	7 cut(s)	148, 282, 408, 612, 683, 726, 780
DpnI	GATC	8 cut(s)	39, 85, 300, 483, 756, 861, 1147, 1257
DpnII	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
DrdI	GACNNNNNNGTC	1 cut(s)	158
DseDI	GACNNNNNNGTC	1 cut(s)	158
EaeI	YGGCCR	1 cut(s)	895
Eam1104I	CTCTTC	3 cut(s)	929, 980, 1190
EarI	CTCTTC	3 cut(s)	929, 980, 1190
EciI	GGCGGA	1 cut(s)	4
Eco130I	CCWWGG	3 cut(s)	264, 414, 563
Eco24I	GRGCYC	1 cut(s)	1202
Eco47I	GGWCC	2 cut(s)	709, 1012
Eco57I	CTGAAG	1 cut(s)	1178
EcoO109I	RGGNCCY	1 cut(s)	709
EcoRI	GAATTC	1 cut(s)	810
EcoRII	CCWGG	2 cut(s)	129, 958
EcoT14I	CCWWGG	3 cut(s)	264, 414, 563
EcoT38I	GRGCYC	1 cut(s)	1202
ErhI	CCWWGG	3 cut(s)	264, 414, 563
Esp3I	CGTCTC	1 cut(s)	657
FaeI	CATG	9 cut(s)	68, 418, 461, 492, 692, 745, 910, 934, 1120
FaqI	GGGAC	2 cut(s)	394, 617
FatI	CATG	9 cut(s)	64, 414, 457, 488, 688, 741, 906, 930, 1116
FauI	CCCGC	3 cut(s)	336, 401, 1235
FbaI	TGATCA	3 cut(s)	481, 859, 1255
Fnu4HI	GCNGC	3 cut(s)	18, 984, 1269
FokI	GGATG	3 cut(s)	257, 474, 956
FriOI	GRGCYC	1 cut(s)	1202
Fsp4HI	GCNGC	3 cut(s)	18, 984, 1269
FspBI	CTAG	1 cut(s)	923
GluI	GCNGC	3 cut(s)	18, 984, 1269
GsaI	CCCAGC	2 cut(s)	380, 1275
GsuI	CTGGAG	1 cut(s)	981
HaeIII	GGCC	8 cut(s)	134, 263, 269, 392, 413, 848, 897, 1293
HapII	CCGG	8 cut(s)	81, 468, 713, 815, 845, 919, 1143, 1192
Hin1II	CATG	9 cut(s)	68, 418, 461, 492, 692, 745, 910, 934, 1120
HincII	GTYRAC	1 cut(s)	747
HindII	GTYRAC	1 cut(s)	747
HinfI	GANTC	6 cut(s)	50, 945, 998, 1037, 1100, 1109
HpaI	GTTAAC	1 cut(s)	747
HpaII	CCGG	8 cut(s)	81, 468, 713, 815, 845, 919, 1143, 1192
HphI	GGTGA	2 cut(s)	707, 834
Hpy166II	GTNNAC	3 cut(s)	747, 904, 1069
Hpy188I	TCNGA	3 cut(s)	797, 873, 1158
Hpy8I	GTNNAC	3 cut(s)	747, 904, 1069
Hpy99I	CGWCG	1 cut(s)	1325
HpyAV	CCTTC	6 cut(s)	85, 253, 503, 724, 1114, 1304
HpyCH4III	ACNGT	2 cut(s)	751, 1183
HpyCH4V	TGCA	7 cut(s)	295, 353, 428, 465, 767, 986, 1027
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 275, 666, 936, 1197, 1206, 1268
HpyF3I	CTNAG	7 cut(s)	148, 282, 408, 612, 683, 726, 780
Hsp92II	CATG	9 cut(s)	68, 418, 461, 492, 692, 745, 910, 934, 1120
Kpn2I	TCCGGA	1 cut(s)	80
Ksp22I	TGATCA	3 cut(s)	481, 859, 1255
KspAI	GTTAAC	1 cut(s)	747
Kzo9I	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
LguI	GCTCTTC	2 cut(s)	929, 1190
LmnI	GCTCC	4 cut(s)	17, 440, 565, 1060
Lsp1109I	GCAGC	2 cut(s)	970, 1255
LweI	GCATC	6 cut(s)	436, 452, 586, 754, 1238, 1255
MaeI	CTAG	1 cut(s)	923
MaeIII	GTNAC	5 cut(s)	637, 695, 1150, 1177, 1183
MalI	GATC	8 cut(s)	39, 85, 300, 483, 756, 861, 1147, 1257
MbiI	CCGCTC	1 cut(s)	1242
MboI	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
MboII	GAAGA	5 cut(s)	605, 773, 946, 967, 1207
MflI	RGATCY	2 cut(s)	83, 298
MhlI	GDGCHC	1 cut(s)	1202
MluCI	AATT	7 cut(s)	113, 239, 498, 670, 798, 810, 891
MlyI	GAGTC	4 cut(s)	954, 1007, 1031, 1109
MmeI	TCCRAC	1 cut(s)	418
MroI	TCCGGA	1 cut(s)	80
MroXI	GAANNNNTTC	1 cut(s)	802
MseI	TTAA	4 cut(s)	176, 746, 1004, 1032
MslI	CAYNNNNRTG	1 cut(s)	693
MspI	CCGG	8 cut(s)	81, 468, 713, 815, 845, 919, 1143, 1192
MspR9I	CCNGG	4 cut(s)	131, 469, 713, 960
MvaI	CCWGG	2 cut(s)	131, 960
MvnI	CGCG	2 cut(s)	1058, 1098
MwoI	GCNNNNNNNGC	7 cut(s)	14, 275, 666, 936, 1197, 1206, 1268
NciI	CCSGG	2 cut(s)	469, 713
NcoI	CCATGG	1 cut(s)	414
NdeII	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
NlaIII	CATG	9 cut(s)	68, 418, 461, 492, 692, 745, 910, 934, 1120
NlaIV	GGNNCC	5 cut(s)	85, 561, 680, 711, 967
NmuCI	GTSAC	3 cut(s)	637, 695, 1183
NruI	TCGCGA	1 cut(s)	1098
NspI	RCATGY	1 cut(s)	492
PaeI	GCATGC	1 cut(s)	492
PagI	TCATGA	2 cut(s)	688, 1116
PaqCI	CACCTGC	2 cut(s)	338, 1068
PciSI	GCTCTTC	2 cut(s)	929, 1190
PcsI	WCGNNNNNNNCGW	1 cut(s)	1233
PdmI	GAANNNNTTC	1 cut(s)	802
PfeI	GAWTC	2 cut(s)	50, 1109
PfoI	TCCNGGA	1 cut(s)	958
PkrI	GCNGC	3 cut(s)	19, 985, 1270
PleI	GAGTC	4 cut(s)	953, 1006, 1031, 1108
PpsI	GAGTC	4 cut(s)	953, 1006, 1031, 1108
PpuMI	RGGWCCY	1 cut(s)	709
PshAI	GACNNNNGTC	1 cut(s)	74
Psp5II	RGGWCCY	1 cut(s)	709
Psp6I	CCWGG	2 cut(s)	129, 958
PspFI	CCCAGC	2 cut(s)	376, 1271
PspGI	CCWGG	2 cut(s)	129, 958
PspN4I	GGNNCC	5 cut(s)	85, 561, 680, 711, 967
PspPI	GGNCC	3 cut(s)	391, 709, 1012
PspPPI	RGGWCCY	1 cut(s)	709
PsuI	RGATCY	2 cut(s)	83, 298
RruI	TCGCGA	1 cut(s)	1098
RsaI	GTAC	4 cut(s)	1055, 1070, 1166, 1214
RsaNI	GTAC	4 cut(s)	1054, 1069, 1165, 1213
RseI	CAYNNNNRTG	1 cut(s)	693
SapI	GCTCTTC	2 cut(s)	929, 1190
SaqAI	TTAA	4 cut(s)	176, 746, 1004, 1032
SatI	GCNGC	3 cut(s)	18, 984, 1269
Sau3AI	GATC	8 cut(s)	37, 83, 298, 481, 754, 859, 1145, 1255
Sau96I	GGNCC	3 cut(s)	391, 709, 1012
SchI	GAGTC	4 cut(s)	954, 1007, 1031, 1109
ScrFI	CCNGG	4 cut(s)	131, 469, 713, 960
SduI	GDGCHC	1 cut(s)	1202
SfaNI	GCATC	6 cut(s)	436, 452, 586, 754, 1238, 1255
SfcI	CTRYAG	2 cut(s)	136, 450
SinI	GGWCC	2 cut(s)	709, 1012
SmiMI	CAYNNNNRTG	1 cut(s)	693
SmlI	CTYRAG	2 cut(s)	995, 1049
SmoI	CTYRAG	2 cut(s)	995, 1049
SphI	GCATGC	1 cut(s)	492
Sse9I	AATT	7 cut(s)	113, 239, 498, 670, 798, 810, 891
SsiI	CCGC	7 cut(s)	6, 15, 18, 343, 394, 1058, 1242
SspMI	CTAG	1 cut(s)	923
StyD4I	CCNGG	4 cut(s)	129, 467, 711, 958
StyI	CCWWGG	3 cut(s)	264, 414, 563
TaaI	ACNGT	2 cut(s)	751, 1183
TaqI	TCGA	5 cut(s)	753, 1086, 1236, 1309, 1320
TaqII	GACCGA	2 cut(s)	811, 1029
TasI	AATT	7 cut(s)	113, 239, 498, 670, 798, 810, 891
TatI	WGTACW	2 cut(s)	1164, 1212
TauI	GCSGC	1 cut(s)	20
TfiI	GAWTC	2 cut(s)	50, 1109
Tru1I	TTAA	4 cut(s)	176, 746, 1004, 1032
Tru9I	TTAA	4 cut(s)	176, 746, 1004, 1032
TscAI	CASTG	5 cut(s)	217, 277, 642, 985, 1014
TseFI	GTSAC	3 cut(s)	637, 695, 1183
TseI	GCWGC	2 cut(s)	983, 1268
Tsp45I	GTSAC	3 cut(s)	637, 695, 1183
TspDTI	ATGAA	7 cut(s)	730, 789, 947, 966, 1105, 1133, 1267
TspGWI	ACGGA	2 cut(s)	159, 903
TspRI	CASTG	5 cut(s)	217, 277, 642, 985, 1014
VpaK11BI	GGWCC	2 cut(s)	709, 1012
XapI	RAATTY	1 cut(s)	810
XceI	RCATGY	1 cut(s)	492
XmnI	GAANNNNTTC	1 cut(s)	802
XspI	CTAG	1 cut(s)	923

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.