Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0004712

Physical Location & Seq

Genomic Coordinates Forward (+)

8534769 .. 8537971

Gene Structure 3203 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG113800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGGCACAGTTGAACACCAACATCGCCTTTCACGCTCCAAAATCAGCACTGCCCAGTTACAGTGAGACTGAAGTAGCTGGATCTACAAAGGAAAGAGGATTACGTGGCAAATTGAACAAGGTTGTTCTGGCCTATAGTGGTGGCTTAGACACGTCAGTCATTGTCCCATGGCTAAGGGAGAACTATGGTTGTGAAGTTGTTTGCTTCACTGCCGACGTGGGTCAAGGTATAAAAGAATTGGATGGTCTGGAACAGAAGGCCAAGGCAAGTGGGGCTTGTCAGTTAGTAGTGAAGGATCTGAAGGAGGAGTTTGTAAAAGACTATGTATTCCCTTGCTTGCGAGCTGGTGCAATCTATGAGAGGAAATACTTGCTTGGGACCTCAATGGCCCGTCCTGTTATTGCTAAGGCCATGGTAGATGTTGCCAAAGCAGTTGGAGCTGATGCCGTCGCTCATGGATGCACAGGGAAAGGAAATGATCAGGTTCGGTTTGAGCTGACATTTTTTGCTCTCGACCCCAAACTTAATGTGGTGGCTCCTTGGAGGGAATGGGACATTACGGGTAGAGAAGATGCTATTGAATATGCTAAGAAGCATAATGTGCCTGTCCCAGTGACAAAGAAATCCATATACAGCAGAGATGGGAACTTATGGCACCTTAGTCATGAGGGTGATATTTTGGAAGACCCGGAAAATGAGCCGAAGAAGGATATGTTCATGTTATCTGTTGATCCAGAAGATGCACCGAATGAACCTGAGTATGTAGATATCGGGATCGTTTCAGGAATTCCAGTTTCAGTCGATGGACAAGAGCTTTCACCAGCTTCTTTGCTTACGAAGCTTAATGAGATTGGCGGAAGACACGGAATTGGTCGTATTGACATGGTTGAAAACCGTCTTGTTGGTATGAAGAGCCGTGGAGTCTATGAAACTCCTGGAGGCACCATCCTATTCGCTGCTGCACGAGAGTTGGAGTCCTTAACTTTGGATCGAGAAACAATGCAAGTTAAAGACTCGCTTGCCCTCAAATATGCAGAGCTAGTCTATGCAGGCAGGTGGTTCGACCCACTTCGTGAGTCCATGGATGCATTCATGGAGAAAATCTCGGAGACAACAACGGGATCAGTAACTCTAAAGTTGTACAAGGGTTCGATCACTGTAACTAGCCGTAAGAGTCCATATAGCCTGTATAGGCAAGACATCTCTTCATTTGAGAGCGGAGATGTGTATGATCAAGCCGATGCAGCTGGGTTTATTAGGCTATATGGTCTCCCCATGAGGGTGCGGGCAATGCTTAAGGAGGGGATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0000053 GO:0003674 GO:0003824 GO:0004055 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006575 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0019627 GO:0019752 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0071941 GO:0072350 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko01100 ko01110 ko01130 ko01230 ko05418 map00220 map00250 map01100 map01110 map01130 map01230 map05418

Pfam Domains

Protein Families

Arginosuc_synth

Protein Analysis

436

Amino Acids

48.02

Weight (kDa)

5.43

Isoelectric Point (pI)

30.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arginosuc_synth	PF00764	40 - 202	1.1e-68	Arginosuccinate synthase N-terminal HUP domain
Arginosuc_syn_C	PF20979	211 - 428	2.9e-82	Arginosuccinate synthase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24830 AT4G24830
fragaria_vesca	FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g30030
malus_domestica	MD06G1144000.v1.1 MD06G1144100.v1.1 MD14G1159300.v1.1
prunus_persica	Prupe.5G153100_v2.0.a1 Prupe.5G153100_v2.0.a1 Prupe.7G058500_v2.0.a1
pyrus_communis	pycom06g13360 pycom14g13300
rosa_chinensis	RchiOBHm_Chr7g0190471 RchiOBHm_Chr7g0190481
rosa_laevigata	RLG00000004524 RLG00000004526
rosa_multiflora	Rmu_sc0000526.1_g000010
rosa_roxburghii	Rroxscaffold_3G00264570
rosa_rugosa	Rorug06G0504800
rosa_samantha	Rh7AG110900 Rh7BG113700 Rh7BG113800 Rh7CG115900 Rh7DG114600 Rh7DG114700
rosa_wichuraiana	Rw0G023270 Rw7G009670 Rw7G009680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1044
AasI	GACNNNNNNGTC	1 cut(s)	155
Acc36I	ACCTGC	1 cut(s)	1044
AccB1I	GGYRCC	2 cut(s)	654, 941
AccBSI	CCGCTC	1 cut(s)	1218
AciI	CCGC	3 cut(s)	855, 1218, 1285
AclWI	GGATC	6 cut(s)	88, 303, 725, 782, 996, 1129
AcsI	RAATTY	1 cut(s)	786
AcuI	CTGAAG	2 cut(s)	90, 320
AdeI	CACNNNGTG	1 cut(s)	1073
AfaI	GTAC	1 cut(s)	1142
AfiI	CCNNNNNNNGG	1 cut(s)	1279
AflII	CTTAAG	1 cut(s)	1295
AflIII	ACRYGT	1 cut(s)	150
AgsI	TTSAA	4 cut(s)	13, 115, 581, 890
AjiI	CACGTC	2 cut(s)	153, 217
AjnI	CCWGG	1 cut(s)	934
AluBI	AGCT	9 cut(s)	77, 344, 440, 496, 814, 824, 841, 1039, 1247
AluI	AGCT	9 cut(s)	77, 344, 440, 496, 814, 824, 841, 1039, 1247
Alw26I	GTCTC	3 cut(s)	59, 1103, 1274
AlwI	GGATC	6 cut(s)	88, 303, 725, 782, 996, 1129
AoxI	GGCC	4 cut(s)	129, 258, 387, 408
ApeKI	GCWGC	3 cut(s)	956, 959, 1244
ApoI	RAATTY	1 cut(s)	786
AspS9I	GGNCC	2 cut(s)	378, 388
AsuC2I	CCSGG	1 cut(s)	689
AsuHPI	GGTGA	2 cut(s)	683, 810
AvaII	GGWCC	1 cut(s)	378
BanI	GGYRCC	2 cut(s)	654, 941
BauI	CACGAG	1 cut(s)	963
BbsI	GAAGAC	2 cut(s)	690, 865
BbvI	GCAGC	3 cut(s)	943, 946, 1256
BccI	CCATC	4 cut(s)	236, 635, 797, 953
BceAI	ACGGC	3 cut(s)	431, 900, 1152
BcgI	CGANNNNNNTGC	2 cut(s)	1042, 1076
BciT130I	CCWGG	1 cut(s)	936
BclI	TGATCA	2 cut(s)	478, 1231
BcnI	CCSGG	1 cut(s)	689
BcoDI	GTCTC	3 cut(s)	59, 1103, 1274
BfaI	CTAG	3 cut(s)	1040, 1164, 1309
BfmI	CTRYAG	1 cut(s)	133
BfrI	CTTAAG	1 cut(s)	1295
BfuAI	ACCTGC	1 cut(s)	1044
BisI	GCNGC	3 cut(s)	957, 960, 1245
BlsI	GCNGC	3 cut(s)	958, 961, 1246
Bme1390I	CCNGG	2 cut(s)	689, 936
Bme18I	GGWCC	1 cut(s)	378
BmgBI	CACGTC	2 cut(s)	153, 217
BmgT120I	GGNCC	2 cut(s)	378, 388
BmiI	GGNNCC	4 cut(s)	379, 537, 656, 943
BmrFI	CCNGG	2 cut(s)	689, 936
BmrI	ACTGGG	2 cut(s)	48, 605
BmsI	GCATC	6 cut(s)	433, 449, 562, 730, 1075, 1231
BmuI	ACTGGG	2 cut(s)	48, 605
BoxI	GACNNNNGTC	1 cut(s)	219
BpiI	GAAGAC	2 cut(s)	690, 865
BplI	GAGNNNNNCTC	2 cut(s)	1088, 1120
BpmI	CTGGAG	1 cut(s)	957
Bpu10I	CCTNAGC	2 cut(s)	173, 405
BpuMI	CCSGG	1 cut(s)	689
BsaAI	YACGTR	1 cut(s)	104
BsaI	GGTCTC	1 cut(s)	1274
BsaJI	CCNNGG	6 cut(s)	167, 261, 411, 539, 916, 1080
Bsc4I	CCNNNNNNNGG	1 cut(s)	1279
Bse1I	ACTGG	3 cut(s)	54, 611, 791
Bse3DI	GCAATG	1 cut(s)	1296
BseBI	CCWGG	1 cut(s)	936
BseDI	CCNNGG	6 cut(s)	167, 261, 411, 539, 916, 1080
BseGI	GGATG	4 cut(s)	247, 464, 945, 1090
BseLI	CCNNNNNNNGG	1 cut(s)	1279
BseMI	GCAATG	1 cut(s)	1296
BseMII	CTCAG	1 cut(s)	747
BseNI	ACTGG	3 cut(s)	54, 611, 791
BseRI	GAGGAG	1 cut(s)	320
BseXI	GCAGC	3 cut(s)	943, 946, 1256
BseYI	CCCAGC	1 cut(s)	1247
BsgI	GTGCAG	1 cut(s)	945
BshFI	GGCC	4 cut(s)	131, 260, 389, 410
BshNI	GGYRCC	2 cut(s)	654, 941
BsiSI	CCGG	1 cut(s)	689
BslFI	GGGAC	4 cut(s)	149, 391, 566, 593
BslI	CCNNNNNNNGG	1 cut(s)	1279
BsmAI	GTCTC	3 cut(s)	59, 1103, 1274
BsmFI	GGGAC	4 cut(s)	149, 391, 566, 593
BsmI	GAATGC	1 cut(s)	1088
BsnI	GGCC	4 cut(s)	131, 260, 389, 410
Bso31I	GGTCTC	1 cut(s)	1274
Bsp1407I	TGTACA	1 cut(s)	1140
Bsp19I	CCATGG	3 cut(s)	167, 411, 1080
BspACI	CCGC	3 cut(s)	855, 1218, 1285
BspANI	GGCC	4 cut(s)	131, 260, 389, 410
BspCNI	CTCAG	1 cut(s)	748
BspHI	TCATGA	1 cut(s)	664
BspLI	GGNNCC	4 cut(s)	379, 537, 656, 943
BspMI	ACCTGC	1 cut(s)	1044
BspPI	GGATC	6 cut(s)	88, 303, 725, 782, 996, 1129
BspQI	GCTCTTC	1 cut(s)	905
BspT107I	GGYRCC	2 cut(s)	654, 941
BspTI	CTTAAG	1 cut(s)	1295
BspTNI	GGTCTC	1 cut(s)	1274
BsrBI	CCGCTC	1 cut(s)	1218
BsrDI	GCAATG	1 cut(s)	1296
BsrGI	TGTACA	1 cut(s)	1140
BsrI	ACTGG	3 cut(s)	54, 611, 791
BssECI	CCNNGG	6 cut(s)	167, 261, 411, 539, 916, 1080
BssSI	CACGAG	1 cut(s)	963
BssT1I	CCWWGG	5 cut(s)	167, 261, 411, 539, 1080
Bst2BI	CACGAG	1 cut(s)	963
Bst2UI	CCWGG	1 cut(s)	936
Bst4CI	ACNGT	4 cut(s)	9, 62, 896, 1159
Bst6I	CTCTTC	2 cut(s)	905, 1210
BstAFI	CTTAAG	1 cut(s)	1295
BstAPI	GCANNNNNTGC	1 cut(s)	601
BstAUI	TGTACA	1 cut(s)	1140
BstBAI	YACGTR	1 cut(s)	104
BstC8I	GCNNGC	5 cut(s)	338, 342, 1020, 1051, 1287
BstDEI	CTNAG	6 cut(s)	145, 173, 405, 588, 659, 756
BstDSI	CCRYGG	4 cut(s)	167, 411, 916, 1080
BstF5I	GGATG	4 cut(s)	247, 464, 945, 1090
BstMAI	GTCTC	3 cut(s)	59, 1103, 1274
BstMWI	GCNNNNNNNGC	7 cut(s)	32, 272, 437, 601, 838, 1244, 1291
BstNI	CCWGG	1 cut(s)	936
BstPAI	GACNNNNGTC	1 cut(s)	219
BstSCI	CCNGG	2 cut(s)	687, 934
BstSFI	CTRYAG	1 cut(s)	133
BstV1I	GCAGC	3 cut(s)	943, 946, 1256
BstV2I	GAAGAC	2 cut(s)	690, 865
BstX2I	RGATCY	3 cut(s)	80, 295, 1305
BstYI	RGATCY	3 cut(s)	80, 295, 1305
BsuRI	GGCC	4 cut(s)	131, 260, 389, 410
BtgI	CCRYGG	4 cut(s)	167, 411, 916, 1080
BtgZI	GCGATG	1 cut(s)	7
BtrI	CACGTC	2 cut(s)	153, 217
BtsCI	GGATG	4 cut(s)	247, 464, 945, 1090
BtsI	GCAGTG	2 cut(s)	47, 207
BtsIMutI	CAGTG	5 cut(s)	47, 67, 207, 618, 1155
BveI	ACCTGC	1 cut(s)	1044
Cac8I	GCNNGC	5 cut(s)	338, 342, 1020, 1051, 1287
CciI	TCATGA	1 cut(s)	664
Cfr13I	GGNCC	2 cut(s)	378, 388
Csp6I	GTAC	1 cut(s)	1141
CspCI	CAANNNNNGTGG	2 cut(s)	250, 285
CviAII	CATG	9 cut(s)	168, 412, 455, 665, 718, 883, 1081, 1093, 1276
CviQI	GTAC	1 cut(s)	1141
DdeI	CTNAG	6 cut(s)	145, 173, 405, 588, 659, 756
DraIII	CACNNNGTG	1 cut(s)	1073
DrdI	GACNNNNNNGTC	1 cut(s)	155
DseDI	GACNNNNNNGTC	1 cut(s)	155
Eam1104I	CTCTTC	2 cut(s)	905, 1210
EarI	CTCTTC	2 cut(s)	905, 1210
EciI	GGCGGA	1 cut(s)	870
Eco130I	CCWWGG	5 cut(s)	167, 261, 411, 539, 1080
Eco31I	GGTCTC	1 cut(s)	1274
Eco32I	GATATC	1 cut(s)	769
Eco47I	GGWCC	1 cut(s)	378
Eco57I	CTGAAG	2 cut(s)	90, 320
EcoO109I	RGGNCCY	1 cut(s)	378
EcoRI	GAATTC	1 cut(s)	786
EcoRII	CCWGG	1 cut(s)	934
EcoRV	GATATC	1 cut(s)	769
EcoT14I	CCWWGG	5 cut(s)	167, 261, 411, 539, 1080
EcoT22I	ATGCAT	1 cut(s)	1090
ErhI	CCWWGG	5 cut(s)	167, 261, 411, 539, 1080
FaeI	CATG	9 cut(s)	171, 415, 458, 668, 721, 886, 1084, 1096, 1279
FalI	AAGNNNNNCTT	4 cut(s)	259, 291, 1002, 1034
FaqI	GGGAC	4 cut(s)	149, 391, 566, 593
FatI	CATG	9 cut(s)	167, 411, 454, 664, 717, 882, 1080, 1092, 1275
FauI	CCCGC	1 cut(s)	1278
FbaI	TGATCA	2 cut(s)	478, 1231
Fnu4HI	GCNGC	3 cut(s)	957, 960, 1245
FokI	GGATG	4 cut(s)	254, 471, 932, 1097
Fsp4HI	GCNGC	3 cut(s)	957, 960, 1245
FspBI	CTAG	3 cut(s)	1040, 1164, 1309
GluI	GCNGC	3 cut(s)	957, 960, 1245
GsaI	CCCAGC	1 cut(s)	1251
GsuI	CTGGAG	1 cut(s)	957
HaeIII	GGCC	4 cut(s)	131, 260, 389, 410
HapII	CCGG	1 cut(s)	689
Hin1II	CATG	9 cut(s)	171, 415, 458, 668, 721, 886, 1084, 1096, 1279
HindIII	AAGCTT	1 cut(s)	839
HinfI	GANTC	5 cut(s)	921, 974, 1013, 1076, 1174
HpaII	CCGG	1 cut(s)	689
HphI	GGTGA	2 cut(s)	683, 810
Hpy188I	TCNGA	2 cut(s)	300, 1108
Hpy188III	TCNNGA	8 cut(s)	248, 512, 665, 734, 772, 783, 992, 1073
Hpy99I	CGWCG	2 cut(s)	218, 452
HpyAV	CCTTC	4 cut(s)	250, 286, 295, 700
HpyCH4III	ACNGT	4 cut(s)	9, 62, 896, 1159
HpyCH4IV	ACGT	3 cut(s)	103, 152, 216
HpyCH4V	TGCA	9 cut(s)	350, 462, 743, 962, 1003, 1034, 1049, 1088, 1244
HpyF10VI	GCNNNNNNNGC	7 cut(s)	32, 272, 437, 601, 838, 1244, 1291
HpyF3I	CTNAG	6 cut(s)	145, 173, 405, 588, 659, 756
HpySE526I	ACGT	3 cut(s)	103, 152, 216
Hsp92II	CATG	9 cut(s)	171, 415, 458, 668, 721, 886, 1084, 1096, 1279
Ksp22I	TGATCA	2 cut(s)	478, 1231
LguI	GCTCTTC	1 cut(s)	905
LmnI	GCTCC	3 cut(s)	40, 437, 541
Lsp1109I	GCAGC	3 cut(s)	943, 946, 1256
LweI	GCATC	6 cut(s)	433, 449, 562, 730, 1075, 1231
MaeI	CTAG	3 cut(s)	1040, 1164, 1309
MaeII	ACGT	3 cut(s)	103, 152, 216
MaeIII	GTNAC	4 cut(s)	56, 613, 1126, 1159
MbiI	CCGCTC	1 cut(s)	1218
MboII	GAAGA	7 cut(s)	581, 695, 715, 749, 870, 922, 1197
MflI	RGATCY	3 cut(s)	80, 295, 1305
MluCI	AATT	4 cut(s)	110, 236, 786, 867
MlyI	GAGTC	5 cut(s)	930, 983, 1007, 1085, 1183
MmeI	TCCRAC	2 cut(s)	415, 951
Mph1103I	ATGCAT	1 cut(s)	1090
MseI	TTAA	5 cut(s)	525, 843, 980, 1008, 1296
MslI	CAYNNNNRTG	2 cut(s)	669, 1280
MspA1I	CMGCKG	1 cut(s)	1247
MspCI	CTTAAG	1 cut(s)	1295
MspI	CCGG	1 cut(s)	689
MspR9I	CCNGG	2 cut(s)	689, 936
Mva1269I	GAATGC	1 cut(s)	1088
MvaI	CCWGG	1 cut(s)	936
MwoI	GCNNNNNNNGC	7 cut(s)	32, 272, 437, 601, 838, 1244, 1291
NciI	CCSGG	1 cut(s)	689
NcoI	CCATGG	3 cut(s)	167, 411, 1080
NlaIII	CATG	9 cut(s)	171, 415, 458, 668, 721, 886, 1084, 1096, 1279
NlaIV	GGNNCC	4 cut(s)	379, 537, 656, 943
NmuCI	GTSAC	1 cut(s)	613
NsiI	ATGCAT	1 cut(s)	1090
PagI	TCATGA	1 cut(s)	664
PaqCI	CACCTGC	1 cut(s)	1044
PciSI	GCTCTTC	1 cut(s)	905
PctI	GAATGC	1 cut(s)	1088
PfoI	TCCNGGA	1 cut(s)	934
PkrI	GCNGC	3 cut(s)	958, 961, 1246
PleI	GAGTC	5 cut(s)	929, 982, 1007, 1084, 1182
PpsI	GAGTC	5 cut(s)	929, 982, 1007, 1084, 1182
Ppu21I	YACGTR	1 cut(s)	104
PpuMI	RGGWCCY	1 cut(s)	378
PshAI	GACNNNNGTC	1 cut(s)	219
Psp5II	RGGWCCY	1 cut(s)	378
Psp6I	CCWGG	1 cut(s)	934
PspFI	CCCAGC	1 cut(s)	1247
PspGI	CCWGG	1 cut(s)	934
PspN4I	GGNNCC	4 cut(s)	379, 537, 656, 943
PspPI	GGNCC	2 cut(s)	378, 388
PspPPI	RGGWCCY	1 cut(s)	378
PsuI	RGATCY	3 cut(s)	80, 295, 1305
PvuII	CAGCTG	1 cut(s)	1247
RsaI	GTAC	1 cut(s)	1142
RsaNI	GTAC	1 cut(s)	1141
RseI	CAYNNNNRTG	2 cut(s)	669, 1280
SapI	GCTCTTC	1 cut(s)	905
SaqAI	TTAA	5 cut(s)	525, 843, 980, 1008, 1296
SatI	GCNGC	3 cut(s)	957, 960, 1245
Sau96I	GGNCC	2 cut(s)	378, 388
SchI	GAGTC	5 cut(s)	930, 983, 1007, 1085, 1183
ScrFI	CCNGG	2 cut(s)	689, 936
SfaNI	GCATC	6 cut(s)	433, 449, 562, 730, 1075, 1231
SfcI	CTRYAG	1 cut(s)	133
SinI	GGWCC	1 cut(s)	378
SmiMI	CAYNNNNRTG	2 cut(s)	669, 1280
SmlI	CTYRAG	1 cut(s)	1295
SmoI	CTYRAG	1 cut(s)	1295
Sse9I	AATT	4 cut(s)	110, 236, 786, 867
SsiI	CCGC	3 cut(s)	855, 1218, 1285
SspMI	CTAG	3 cut(s)	1040, 1164, 1309
StyD4I	CCNGG	2 cut(s)	687, 934
StyI	CCWWGG	5 cut(s)	167, 261, 411, 539, 1080
TaaI	ACNGT	4 cut(s)	9, 62, 896, 1159
TaiI	ACGT	3 cut(s)	106, 155, 219
TaqI	TCGA	5 cut(s)	513, 801, 991, 1062, 1151
TasI	AATT	4 cut(s)	110, 236, 786, 867
TatI	WGTACW	1 cut(s)	1140
Tru1I	TTAA	5 cut(s)	525, 843, 980, 1008, 1296
Tru9I	TTAA	5 cut(s)	525, 843, 980, 1008, 1296
TscAI	CASTG	5 cut(s)	54, 67, 214, 618, 1162
TseFI	GTSAC	1 cut(s)	613
TseI	GCWGC	3 cut(s)	956, 959, 1244
Tsp45I	GTSAC	1 cut(s)	613
TspDTI	ATGAA	6 cut(s)	706, 765, 923, 942, 1081, 1197
TspGWI	ACGGA	1 cut(s)	879
TspRI	CASTG	5 cut(s)	54, 67, 214, 618, 1162
Vha464I	CTTAAG	1 cut(s)	1295
VpaK11BI	GGWCC	1 cut(s)	378
XapI	RAATTY	1 cut(s)	786
XcmI	CCANNNNNNNNNTGG	1 cut(s)	526
XspI	CTAG	3 cut(s)	1040, 1164, 1309
Zsp2I	ATGCAT	1 cut(s)	1090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.