Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004712

Physical Location & Seq

Genomic Coordinates Forward (+)

28750109 .. 28753650

Gene Structure 3542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1144100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGTCCGCCTGCTCCGCCGCCAATCTCACTTTTCAAGCTCCCAAAAGAGAATCATATACATTTCATGGCAGAGTTTGTTGTCCGGGGAGGTTATCTTCGTTTCAAGAGTTGGGGGCAAGAAAGAGTGAGCTTCAGGGGACTGCATTTGCAGTTACGAGTGGCAATGGAAGTGTAAGAGCTCATAACAAGCAAGCTATTAGAGCAGCGCTACCCACTTACAATGAGACAGAAGCATCTGGATCCACGAAGGAAAGAGGGTTGCGTGGCAAATTGAATAAGGTTGTCCTGGCCTATAGTGGTGGCTTAGACACATCAGTCATTGTTCCGTGGTTAAGGGAGAACTATGGTTGTGATGTTGTTTGCTTCACGGCTGATGTTGGTCAAGGTATAAAAGAATTGGATGGTCTGGAAAAGAAGGCCAAGGCCAGTGGGGCTTCTCAATTAGTAGTGCATGATCTAAAGGAGGAGTTTGTAAAGGACTACATATTCCCTTGCTTGCGGGCAGGTGCAATATATGAGAGGAAATACTTGCTGGGGACTTCAATGGCCCGCCCCGTTATTGCTAAGGCCATGGTTGATGTTGCGAAAGAAGTTGGAGCGGATGCTGTAGCTCATGGATGCACCGGGAAAGGAAATGATCAGGCATGCCTTCCAATTCAAAGCGTTCGCTTTGAGCTGACGTTTTTCGCTCTCAATCCCAAACTAAATGTTGTGGCTCCTTGGAGGGAGTGGGATATTAGAGGCAGAGAAGATGCTATTGAATACGCTAAGAAACATAATGTGCCTGTCCCAGTGACAAAGAAGTCCATATACAGCAGAGATGGCAATTTATGGCACCTTAGTCATGAGGGTGACATTTTGGAAGACCCGGAGAATGAGCCTAAGAAGGATATGTTCATGTTAACAGTCGATCCAGAAGATGCACCAAATGAACCAGAGTACGTAGAAATCGGAATTGTTTCAGGAATTCCGGTTTCGGTCAATGGGAAAGAGCTTTCACCGGCCTCTCTACTTGATCATCTAAATCAGATTGGTGGAAAACACGGAATTGGCCGTGTGGACATGGTTGAAAACCGGCTAGTTGGCATGAAGAGCCGTGGAGTCTATGAAACTCCCGGGGGCACCATCCTCTTCGCTGCTGCACGAGAGCTTGAGTCTTTAACACTGGACCGAGAAACAATGCAAGTTAAAGACTCGTTAGCCCTCAAGTACGCGGAGCTGGTATACGCAGGAAGGTGGTTCGACCCACTTCGCGAGTCATTGGATTCGTTCATGGAGGAAATCTCGAAAACAACCACTGGATCAGTAACTCTGAAGCTGTACAAGGGTTCGGTCACTGTGACGAGCCGGAAGAGCCCTTATAGCCTGTACAGGCAGGACATCTCGTCGTTCGAGAGCGGGGATATATATGATCAAGCTGATGCTGCTGGGTTTATTAGGCTGTATGGCCTTCCCATTAGGGTTCGAGCAATGCTCGACGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0000053 GO:0003674 GO:0003824 GO:0004055 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006575 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0019627 GO:0019752 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0071941 GO:0072350 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko01100 ko01110 ko01130 ko01230 ko05418 map00220 map00250 map01100 map01110 map01130 map01230 map05418

Pfam Domains

Protein Families

Arginosuc_synth

Protein Analysis

495

Amino Acids

54.23

Weight (kDa)

6.36

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arginosuc_synth	PF00764	93 - 262	2.9e-64	Arginosuccinate synthase N-terminal HUP domain
Arginosuc_syn_C	PF20979	271 - 488	2.5e-82	Arginosuccinate synthase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24830 AT4G24830
fragaria_vesca	FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g30030
malus_domestica	MD06G1144000.v1.1 MD06G1144100.v1.1 MD14G1159300.v1.1
prunus_persica	Prupe.5G153100_v2.0.a1 Prupe.5G153100_v2.0.a1 Prupe.7G058500_v2.0.a1
pyrus_communis	pycom06g13360 pycom14g13300
rosa_chinensis	RchiOBHm_Chr7g0190471 RchiOBHm_Chr7g0190481
rosa_laevigata	RLG00000004524 RLG00000004526
rosa_multiflora	Rmu_sc0000526.1_g000010
rosa_roxburghii	Rroxscaffold_3G00264570
rosa_rugosa	Rorug06G0504800
rosa_samantha	Rh7AG110900 Rh7BG113700 Rh7BG113800 Rh7CG115900 Rh7DG114600 Rh7DG114700
rosa_wichuraiana	Rw0G023270 Rw7G009670 Rw7G009680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	494
AasI	GACNNNNNNGTC	2 cut(s)	314, 802
Acc36I	ACCTGC	1 cut(s)	494
AccB1I	GGYRCC	2 cut(s)	834, 1121
AccBSI	CCGCTC	2 cut(s)	599, 1398
AccI	GTMKAC	1 cut(s)	1224
AccII	CGCG	2 cut(s)	1214, 1254
AciI	CCGC	8 cut(s)	6, 15, 18, 499, 550, 599, 1214, 1398
AclWI	GGATC	4 cut(s)	234, 247, 905, 1309
AcoI	YGGCCR	1 cut(s)	1051
AcsI	RAATTY	1 cut(s)	966
AcuI	CTGAAG	2 cut(s)	116, 1334
AfaI	GTAC	4 cut(s)	941, 1211, 1322, 1370
AfeI	AGCGCT	1 cut(s)	207
AgsI	TTSAA	7 cut(s)	35, 104, 274, 543, 659, 761, 1070
AhdI	GACNNNNNGTC	1 cut(s)	1384
AjnI	CCWGG	1 cut(s)	285
Alw21I	GWGCWC	1 cut(s)	181
Alw26I	GTCTC	1 cut(s)	218
AlwI	GGATC	4 cut(s)	234, 247, 905, 1309
Ama87I	CYCGRG	1 cut(s)	1115
Aor51HI	AGCGCT	1 cut(s)	207
AoxI	GGCC	8 cut(s)	288, 417, 423, 546, 567, 1002, 1051, 1447
ApeKI	GCWGC	4 cut(s)	203, 1136, 1139, 1424
ApoI	RAATTY	1 cut(s)	966
Asp700I	GAANNNNTTC	1 cut(s)	958
AspLEI	GCGC	1 cut(s)	208
AspS9I	GGNCC	2 cut(s)	547, 1168
AsuC2I	CCSGG	5 cut(s)	84, 625, 869, 1116, 1117
AsuHPI	GGTGA	2 cut(s)	863, 990
AvaI	CYCGRG	1 cut(s)	1115
AvaII	GGWCC	1 cut(s)	1168
BaeGI	GKGCMC	1 cut(s)	1124
BamHI	GGATCC	1 cut(s)	239
BanI	GGYRCC	2 cut(s)	834, 1121
BanII	GRGCYC	2 cut(s)	181, 1358
BarI	GAAGNNNNNNTAC	2 cut(s)	794, 826
BauI	CACGAG	1 cut(s)	1143
BbsI	GAAGAC	1 cut(s)	870
Bbv12I	GWGCWC	1 cut(s)	181
BbvI	GCAGC	4 cut(s)	215, 1123, 1126, 1411
BccI	CCATC	3 cut(s)	395, 815, 1133
BceAI	ACGGC	3 cut(s)	384, 1038, 1080
BciT130I	CCWGG	1 cut(s)	287
BclI	TGATCA	3 cut(s)	637, 1015, 1411
BcnI	CCSGG	5 cut(s)	84, 625, 869, 1116, 1117
BcoDI	GTCTC	1 cut(s)	218
BfaI	CTAG	1 cut(s)	1079
BfmI	CTRYAG	2 cut(s)	292, 606
BfoI	RGCGCY	1 cut(s)	209
BfuAI	ACCTGC	1 cut(s)	494
BglI	GCCNNNNNGGC	1 cut(s)	431
BisI	GCNGC	5 cut(s)	18, 204, 1137, 1140, 1425
BlsI	GCNGC	5 cut(s)	19, 205, 1138, 1141, 1426
Bme1390I	CCNGG	6 cut(s)	84, 287, 625, 869, 1116, 1117
Bme18I	GGWCC	1 cut(s)	1168
BmeRI	GACNNNNNGTC	1 cut(s)	1384
BmeT110I	CYCGRG	1 cut(s)	1115
BmgT120I	GGNCC	2 cut(s)	547, 1168
BmiI	GGNNCC	4 cut(s)	241, 717, 836, 1123
BmrFI	CCNGG	6 cut(s)	84, 287, 625, 869, 1116, 1117
BmrI	ACTGGG	1 cut(s)	785
BmsI	GCATC	6 cut(s)	242, 592, 608, 742, 910, 1411
BmuI	ACTGGG	1 cut(s)	785
BpiI	GAAGAC	1 cut(s)	870
BplI	GAGNNNNNCTC	3 cut(s)	1268, 1300, 1458
Bpu10I	CCTNAGC	1 cut(s)	564
BpuEI	CTTGAG	2 cut(s)	1172, 1190
BpuMI	CCSGG	5 cut(s)	84, 625, 869, 1116, 1117
BsaAI	YACGTR	1 cut(s)	943
BsaJI	CCNNGG	8 cut(s)	83, 326, 420, 570, 719, 1096, 1115, 1116
BsaWI	WCCGGW	1 cut(s)	970
Bse118I	RCCGGY	2 cut(s)	1000, 1074
Bse1I	ACTGG	4 cut(s)	426, 791, 1170, 1303
Bse3DI	GCAATG	2 cut(s)	169, 1476
BseBI	CCWGG	1 cut(s)	287
BseDI	CCNNGG	8 cut(s)	83, 326, 420, 570, 719, 1096, 1115, 1116
BseGI	GGATG	4 cut(s)	406, 607, 623, 1125
BseMI	GCAATG	2 cut(s)	169, 1476
BseNI	ACTGG	4 cut(s)	426, 791, 1170, 1303
BseRI	GAGGAG	1 cut(s)	479
BseSI	GKGCMC	1 cut(s)	1124
BseXI	GCAGC	4 cut(s)	215, 1123, 1126, 1411
BseYI	CCCAGC	2 cut(s)	532, 1427
BsgI	GTGCAG	1 cut(s)	1125
Bsh1236I	CGCG	2 cut(s)	1214, 1254
BshFI	GGCC	8 cut(s)	290, 419, 425, 548, 569, 1004, 1053, 1449
BshNI	GGYRCC	2 cut(s)	834, 1121
BsiHKAI	GWGCWC	1 cut(s)	181
BsiHKCI	CYCGRG	1 cut(s)	1115
BsiSI	CCGG	8 cut(s)	83, 624, 869, 971, 1001, 1075, 1116, 1348
BslFI	GGGAC	3 cut(s)	151, 550, 773
BsmAI	GTCTC	1 cut(s)	218
BsmFI	GGGAC	3 cut(s)	151, 550, 773
BsnI	GGCC	8 cut(s)	290, 419, 425, 548, 569, 1004, 1053, 1449
BsoBI	CYCGRG	1 cut(s)	1115
Bsp1286I	GDGCHC	3 cut(s)	181, 1124, 1358
Bsp1407I	TGTACA	2 cut(s)	1320, 1368
Bsp143I	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
Bsp19I	CCATGG	1 cut(s)	570
Bsp68I	TCGCGA	1 cut(s)	1254
BspACI	CCGC	8 cut(s)	6, 15, 18, 499, 550, 599, 1214, 1398
BspANI	GGCC	8 cut(s)	290, 419, 425, 548, 569, 1004, 1053, 1449
BspFNI	CGCG	2 cut(s)	1214, 1254
BspHI	TCATGA	1 cut(s)	844
BspLI	GGNNCC	4 cut(s)	241, 717, 836, 1123
BspMI	ACCTGC	1 cut(s)	494
BspPI	GGATC	4 cut(s)	234, 247, 905, 1309
BspQI	GCTCTTC	2 cut(s)	1085, 1346
BspT107I	GGYRCC	2 cut(s)	834, 1121
BsrBI	CCGCTC	2 cut(s)	599, 1398
BsrDI	GCAATG	2 cut(s)	169, 1476
BsrFI	RCCGGY	2 cut(s)	1000, 1074
BsrGI	TGTACA	2 cut(s)	1320, 1368
BsrI	ACTGG	4 cut(s)	426, 791, 1170, 1303
BssAI	RCCGGY	2 cut(s)	1000, 1074
BssECI	CCNNGG	8 cut(s)	83, 326, 420, 570, 719, 1096, 1115, 1116
BssMI	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
BssNAI	GTATAC	1 cut(s)	1225
BssSI	CACGAG	1 cut(s)	1143
BssT1I	CCWWGG	3 cut(s)	420, 570, 719
Bst1107I	GTATAC	1 cut(s)	1225
Bst2BI	CACGAG	1 cut(s)	1143
Bst2UI	CCWGG	1 cut(s)	287
Bst4CI	ACNGT	2 cut(s)	907, 1339
Bst6I	CTCTTC	3 cut(s)	1085, 1136, 1346
BstAUI	TGTACA	2 cut(s)	1320, 1368
BstBAI	YACGTR	1 cut(s)	943
BstC8I	GCNNGC	6 cut(s)	10, 192, 497, 501, 550, 646
BstDEI	CTNAG	5 cut(s)	304, 564, 768, 839, 882
BstDSI	CCRYGG	3 cut(s)	326, 570, 1096
BstF5I	GGATG	4 cut(s)	406, 607, 623, 1125
BstFNI	CGCG	2 cut(s)	1214, 1254
BstH2I	RGCGCY	1 cut(s)	209
BstHHI	GCGC	1 cut(s)	208
BstKTI	GATC	7 cut(s)	242, 457, 640, 913, 1018, 1304, 1414
BstMAI	GTCTC	1 cut(s)	218
BstMBI	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
BstMWI	GCNNNNNNNGC	8 cut(s)	14, 200, 431, 822, 1092, 1353, 1362, 1424
BstNI	CCWGG	1 cut(s)	287
BstNSI	RCATGY	1 cut(s)	648
BstSCI	CCNGG	6 cut(s)	82, 285, 623, 867, 1114, 1115
BstSFI	CTRYAG	2 cut(s)	292, 606
BstSLI	GKGCMC	1 cut(s)	1124
BstSNI	TACGTA	1 cut(s)	943
BstUI	CGCG	2 cut(s)	1214, 1254
BstV1I	GCAGC	4 cut(s)	215, 1123, 1126, 1411
BstV2I	GAAGAC	1 cut(s)	870
BstX2I	RGATCY	1 cut(s)	239
BstYI	RGATCY	1 cut(s)	239
BstZ17I	GTATAC	1 cut(s)	1225
BsuRI	GGCC	8 cut(s)	290, 419, 425, 548, 569, 1004, 1053, 1449
BtgI	CCRYGG	3 cut(s)	326, 570, 1096
BtsCI	GGATG	4 cut(s)	406, 607, 623, 1125
BtsIMutI	CAGTG	5 cut(s)	433, 798, 1163, 1296, 1335
BtuMI	TCGCGA	1 cut(s)	1254
BveI	ACCTGC	1 cut(s)	494
Cac8I	GCNNGC	6 cut(s)	10, 192, 497, 501, 550, 646
CciI	TCATGA	1 cut(s)	844
CfoI	GCGC	1 cut(s)	208
Cfr10I	RCCGGY	2 cut(s)	1000, 1074
Cfr13I	GGNCC	2 cut(s)	547, 1168
Cfr9I	CCCGGG	1 cut(s)	1115
Csp6I	GTAC	4 cut(s)	940, 1210, 1321, 1369
CspCI	CAANNNNNGTGG	2 cut(s)	409, 444
CviQI	GTAC	4 cut(s)	940, 1210, 1321, 1369
DdeI	CTNAG	5 cut(s)	304, 564, 768, 839, 882
DpnI	GATC	7 cut(s)	241, 456, 639, 912, 1017, 1303, 1413
DpnII	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
DrdI	GACNNNNNNGTC	2 cut(s)	314, 802
DriI	GACNNNNNGTC	1 cut(s)	1384
DseDI	GACNNNNNNGTC	2 cut(s)	314, 802
EaeI	YGGCCR	1 cut(s)	1051
Eam1104I	CTCTTC	3 cut(s)	1085, 1136, 1346
Eam1105I	GACNNNNNGTC	1 cut(s)	1384
EarI	CTCTTC	3 cut(s)	1085, 1136, 1346
EciI	GGCGGA	1 cut(s)	4
Ecl136II	GAGCTC	1 cut(s)	179
Eco105I	TACGTA	1 cut(s)	943
Eco130I	CCWWGG	3 cut(s)	420, 570, 719
Eco24I	GRGCYC	2 cut(s)	181, 1358
Eco47I	GGWCC	1 cut(s)	1168
Eco47III	AGCGCT	1 cut(s)	207
Eco53kI	GAGCTC	1 cut(s)	179
Eco57I	CTGAAG	2 cut(s)	116, 1334
Eco88I	CYCGRG	1 cut(s)	1115
EcoICRI	GAGCTC	1 cut(s)	179
EcoRI	GAATTC	1 cut(s)	966
EcoRII	CCWGG	1 cut(s)	285
EcoT14I	CCWWGG	3 cut(s)	420, 570, 719
EcoT38I	GRGCYC	2 cut(s)	181, 1358
ErhI	CCWWGG	3 cut(s)	420, 570, 719
FaqI	GGGAC	3 cut(s)	151, 550, 773
FauI	CCCGC	3 cut(s)	492, 557, 1391
FbaI	TGATCA	3 cut(s)	637, 1015, 1411
FblI	GTMKAC	1 cut(s)	1224
Fnu4HI	GCNGC	5 cut(s)	18, 204, 1137, 1140, 1425
FokI	GGATG	4 cut(s)	413, 614, 630, 1112
FriOI	GRGCYC	2 cut(s)	181, 1358
Fsp4HI	GCNGC	5 cut(s)	18, 204, 1137, 1140, 1425
FspBI	CTAG	1 cut(s)	1079
GlaI	GCGC	1 cut(s)	207
GluI	GCNGC	5 cut(s)	18, 204, 1137, 1140, 1425
GsaI	CCCAGC	2 cut(s)	536, 1431
HaeII	RGCGCY	1 cut(s)	209
HaeIII	GGCC	8 cut(s)	290, 419, 425, 548, 569, 1004, 1053, 1449
HapII	CCGG	8 cut(s)	83, 624, 869, 971, 1001, 1075, 1116, 1348
HhaI	GCGC	1 cut(s)	208
Hin6I	GCGC	1 cut(s)	206
HinP1I	GCGC	1 cut(s)	206
HincII	GTYRAC	1 cut(s)	903
HindII	GTYRAC	1 cut(s)	903
HinfI	GANTC	6 cut(s)	50, 1101, 1154, 1193, 1256, 1265
HpaI	GTTAAC	1 cut(s)	903
HpaII	CCGG	8 cut(s)	83, 624, 869, 971, 1001, 1075, 1116, 1348
HphI	GGTGA	2 cut(s)	863, 990
Hpy166II	GTNNAC	3 cut(s)	903, 1060, 1225
Hpy188I	TCNGA	3 cut(s)	953, 1029, 1314
Hpy188III	TCNNGA	9 cut(s)	104, 237, 407, 845, 914, 963, 1253, 1285, 1393
Hpy8I	GTNNAC	3 cut(s)	903, 1060, 1225
Hpy99I	CGWCG	2 cut(s)	1390, 1481
HpyAV	CCTTC	6 cut(s)	241, 409, 659, 880, 1227, 1460
HpyCH4III	ACNGT	2 cut(s)	907, 1339
HpyCH4IV	ACGT	2 cut(s)	680, 942
HpyCH4V	TGCA	8 cut(s)	143, 149, 451, 509, 621, 923, 1142, 1183
HpyF10VI	GCNNNNNNNGC	8 cut(s)	14, 200, 431, 822, 1092, 1353, 1362, 1424
HpyF3I	CTNAG	5 cut(s)	304, 564, 768, 839, 882
HpySE526I	ACGT	2 cut(s)	680, 942
HspAI	GCGC	1 cut(s)	206
Ksp22I	TGATCA	3 cut(s)	637, 1015, 1411
KspAI	GTTAAC	1 cut(s)	903
Kzo9I	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
LguI	GCTCTTC	2 cut(s)	1085, 1346
LmnI	GCTCC	5 cut(s)	17, 43, 596, 721, 1216
Lsp1109I	GCAGC	4 cut(s)	215, 1123, 1126, 1411
LweI	GCATC	6 cut(s)	242, 592, 608, 742, 910, 1411
MaeI	CTAG	1 cut(s)	1079
MaeII	ACGT	2 cut(s)	680, 942
MaeIII	GTNAC	6 cut(s)	151, 793, 851, 1306, 1333, 1339
MalI	GATC	7 cut(s)	241, 456, 639, 912, 1017, 1303, 1413
MbiI	CCGCTC	2 cut(s)	599, 1398
MboI	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
MboII	GAAGA	7 cut(s)	87, 761, 875, 929, 1102, 1123, 1363
MflI	RGATCY	1 cut(s)	239
MhlI	GDGCHC	3 cut(s)	181, 1124, 1358
MluCI	AATT	8 cut(s)	269, 395, 440, 654, 826, 954, 966, 1047
MlyI	GAGTC	4 cut(s)	1110, 1163, 1187, 1265
MmeI	TCCRAC	1 cut(s)	574
MroXI	GAANNNNTTC	1 cut(s)	958
MseI	TTAA	4 cut(s)	332, 902, 1160, 1188
MslI	CAYNNNNRTG	1 cut(s)	849
MspI	CCGG	8 cut(s)	83, 624, 869, 971, 1001, 1075, 1116, 1348
MspR9I	CCNGG	6 cut(s)	84, 287, 625, 869, 1116, 1117
MvaI	CCWGG	1 cut(s)	287
MvnI	CGCG	2 cut(s)	1214, 1254
MwoI	GCNNNNNNNGC	8 cut(s)	14, 200, 431, 822, 1092, 1353, 1362, 1424
NciI	CCSGG	5 cut(s)	84, 625, 869, 1116, 1117
NcoI	CCATGG	1 cut(s)	570
NdeII	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
NlaIV	GGNNCC	4 cut(s)	241, 717, 836, 1123
NmuCI	GTSAC	4 cut(s)	793, 851, 1333, 1339
NruI	TCGCGA	1 cut(s)	1254
NspI	RCATGY	1 cut(s)	648
PaeI	GCATGC	1 cut(s)	648
PagI	TCATGA	1 cut(s)	844
PaqCI	CACCTGC	1 cut(s)	494
PciSI	GCTCTTC	2 cut(s)	1085, 1346
PdmI	GAANNNNTTC	1 cut(s)	958
PfeI	GAWTC	2 cut(s)	50, 1265
PkrI	GCNGC	5 cut(s)	19, 205, 1138, 1141, 1426
PleI	GAGTC	4 cut(s)	1109, 1162, 1187, 1264
PpsI	GAGTC	4 cut(s)	1109, 1162, 1187, 1264
Ppu21I	YACGTR	1 cut(s)	943
Psp124BI	GAGCTC	1 cut(s)	181
Psp6I	CCWGG	1 cut(s)	285
PspFI	CCCAGC	2 cut(s)	532, 1427
PspGI	CCWGG	1 cut(s)	285
PspN4I	GGNNCC	4 cut(s)	241, 717, 836, 1123
PspPI	GGNCC	2 cut(s)	547, 1168
PsuI	RGATCY	1 cut(s)	239
RruI	TCGCGA	1 cut(s)	1254
RsaI	GTAC	4 cut(s)	941, 1211, 1322, 1370
RsaNI	GTAC	4 cut(s)	940, 1210, 1321, 1369
RseI	CAYNNNNRTG	1 cut(s)	849
SacI	GAGCTC	1 cut(s)	181
SapI	GCTCTTC	2 cut(s)	1085, 1346
SaqAI	TTAA	4 cut(s)	332, 902, 1160, 1188
SatI	GCNGC	5 cut(s)	18, 204, 1137, 1140, 1425
Sau3AI	GATC	7 cut(s)	239, 454, 637, 910, 1015, 1301, 1411
Sau96I	GGNCC	2 cut(s)	547, 1168
SchI	GAGTC	4 cut(s)	1110, 1163, 1187, 1265
ScrFI	CCNGG	6 cut(s)	84, 287, 625, 869, 1116, 1117
SduI	GDGCHC	3 cut(s)	181, 1124, 1358
SfaNI	GCATC	6 cut(s)	242, 592, 608, 742, 910, 1411
SfcI	CTRYAG	2 cut(s)	292, 606
SinI	GGWCC	1 cut(s)	1168
SmaI	CCCGGG	1 cut(s)	1117
SmiMI	CAYNNNNRTG	1 cut(s)	849
SmlI	CTYRAG	2 cut(s)	1151, 1205
SmoI	CTYRAG	2 cut(s)	1151, 1205
SnaBI	TACGTA	1 cut(s)	943
SphI	GCATGC	1 cut(s)	648
Sse9I	AATT	8 cut(s)	269, 395, 440, 654, 826, 954, 966, 1047
SsiI	CCGC	8 cut(s)	6, 15, 18, 499, 550, 599, 1214, 1398
SspMI	CTAG	1 cut(s)	1079
SstI	GAGCTC	1 cut(s)	181
StyD4I	CCNGG	6 cut(s)	82, 285, 623, 867, 1114, 1115
StyI	CCWWGG	3 cut(s)	420, 570, 719
TaaI	ACNGT	2 cut(s)	907, 1339
TaiI	ACGT	2 cut(s)	683, 945
TaqI	TCGA	6 cut(s)	909, 1242, 1286, 1392, 1465, 1476
TaqII	GACCGA	3 cut(s)	967, 1185, 1321
TasI	AATT	8 cut(s)	269, 395, 440, 654, 826, 954, 966, 1047
TatI	WGTACW	2 cut(s)	1320, 1368
TauI	GCSGC	1 cut(s)	20
TfiI	GAWTC	2 cut(s)	50, 1265
Tru1I	TTAA	4 cut(s)	332, 902, 1160, 1188
Tru9I	TTAA	4 cut(s)	332, 902, 1160, 1188
TscAI	CASTG	5 cut(s)	433, 798, 1170, 1303, 1342
TseFI	GTSAC	4 cut(s)	793, 851, 1333, 1339
TseI	GCWGC	4 cut(s)	203, 1136, 1139, 1424
Tsp45I	GTSAC	4 cut(s)	793, 851, 1333, 1339
TspDTI	ATGAA	6 cut(s)	53, 886, 945, 1103, 1122, 1261
TspGWI	ACGGA	2 cut(s)	315, 1059
TspMI	CCCGGG	1 cut(s)	1115
TspRI	CASTG	5 cut(s)	433, 798, 1170, 1303, 1342
VpaK11BI	GGWCC	1 cut(s)	1168
XapI	RAATTY	1 cut(s)	966
XceI	RCATGY	1 cut(s)	648
XmaI	CCCGGG	1 cut(s)	1115
XmiI	GTMKAC	1 cut(s)	1224
XmnI	GAANNNNTTC	1 cut(s)	958
XspI	CTAG	1 cut(s)	1079

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.