Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004712

Physical Location & Seq

Genomic Coordinates Forward (+)

25329058 .. 25332950

Gene Structure 3893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1159300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGCTCAATTGAAGTCCATGTCCGCCTGCTCCGCCGCCAACCTCACTTTTCACGCGCCAAAAAGAGAATCATATCCGTATCATGGCAGAGTTTGTTGTCCGGGGAAATTATCTTCATTTCAAGAGTTGGGGGCACGAAAGAGTGAGCTGCAGGGGAATGCATTTGCGGTTACCAGTGGCAATGGAAGTGTAAGAGCTCATAACAAGCAAGTTATTAGAGCGGTGCTGCCCACTTACAGTGAGACAGAAGTATCCGGATCCACGAAGGAAAGAGGACTGCGTGGCAAATTGAACAAGGTTGTTCTAGCCTATAGTGGTGGCTTAGACACATCAGTCATTGTCCCATGGTTAAGGGAGAATTATGGTTGTGATGTTGTTTGCTTCACGGCTGATGTTGGTCAAGGTATAAAAGAATTGGATGGTCTGGAAAAAAAGGCCAAAGCCAGTGGGGCTTCTCAGTTAGTAGTGAAGGATCTAAAGGAGGAGTTTGTAAAGGAGTACATATTCCCTTGCTTGCGGGCTGGTGCAATCTATGAGAGAAAATACTTGTTAGGGACTTCAATGGCCCGTCCTGTTATTGCTAAGGTGGCCATGGTTGATGTTGCAAAAGAAGTTGGAGCTGATGCTGTAGCTCATGGATGCACTGGGAAAGGAAATGATCAGGCATGCCTTCCAATTCAGAGCGTTCGCTTTGAGCTGACATTTTTCGCTCTCAATCCCAAACTAAATGTTGTCGCTCCTTGGAGGGAGTGGGATATTACAGGTAGAGAAGATGCTATTGAATACGCTAAGAAGCATAATGTGCCTGTCCCAGTGACAAAGAAATCCATATACAGCAGAGACGGCAATTTATGGCACCTTAGTCATGAGGGTGATATTTTGGAAGACCCGGAAAACGAGCCCAAGAAGGATATGTTCATGTTAACAGTTGATCCAGAGGATGCACCAAATAAACCTGAGCATGTAGAAATTGGAATCGTTTCTGGAATTCCAGTTTCAGTCAATGGGAAGGAGCTTTCGCCGGCATCTCTACTTGATCATCTCAATCAGATTGGTGGAAAACATGGAATTGGCCGCGTTGACATGGTTGAAAACCGGCTAGTCGGTATGAAGAGCCGTGGAGTCTATGAAACTCCTGGAGGCACCATCCTCTTCGCTGCTGCACGCGAGCTGGAGTCTTTAACACTGGACCGAGAAACAATGCAAGTTAAAGACTCACTAGCCCTCAAATACGCGGAGCTGGTATATGCAGGCAGGTGGTTTGACCCACTGCGCGAGTCCTTGGATTCGTTCATGGAGGAAATCTCAAAAACAACCACCGGATCAGTAACTCTGAAGCTGTACAGGGGTTCAGTTACCGTGACCAGCCGGAAGAGCCCGTACAGTCTCTACAGGCAGGACATCTCGTCGTTTGAGAGTGGGGATATATATGATCAGGCTGATGCTGCTGGGTTCATTAGGCTGTATGGCCTTCCCATTAGGGTTCGCGCAATGCTCGACTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0000053 GO:0003674 GO:0003824 GO:0004055 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006575 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0019627 GO:0019752 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0071941 GO:0072350 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko01100 ko01110 ko01130 ko01230 ko05418 map00220 map00250 map01100 map01110 map01130 map01230 map05418

Pfam Domains

Protein Families

Arginosuc_synth

Protein Analysis

502

Amino Acids

55.02

Weight (kDa)

7.13

Isoelectric Point (pI)

34.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arginosuc_synth	PF00764	99 - 269	1e-62	Arginosuccinate synthase N-terminal HUP domain
Arginosuc_syn_C	PF20979	278 - 495	7.6e-82	Arginosuccinate synthase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24830 AT4G24830
fragaria_vesca	FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g30030
malus_domestica	MD06G1144000.v1.1 MD06G1144100.v1.1 MD14G1159300.v1.1
prunus_persica	Prupe.5G153100_v2.0.a1 Prupe.5G153100_v2.0.a1 Prupe.7G058500_v2.0.a1
pyrus_communis	pycom06g13360 pycom14g13300
rosa_chinensis	RchiOBHm_Chr7g0190471 RchiOBHm_Chr7g0190481
rosa_laevigata	RLG00000004524 RLG00000004526
rosa_multiflora	Rmu_sc0000526.1_g000010
rosa_roxburghii	Rroxscaffold_3G00264570
rosa_rugosa	Rorug06G0504800
rosa_samantha	Rh7AG110900 Rh7BG113700 Rh7BG113800 Rh7CG115900 Rh7DG114600 Rh7DG114700
rosa_wichuraiana	Rw0G023270 Rw7G009670 Rw7G009680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1245
AasI	GACNNNNNNGTC	1 cut(s)	332
Acc36I	ACCTGC	1 cut(s)	1245
AccB1I	GGYRCC	2 cut(s)	855, 1142
AccBSI	CCGCTC	1 cut(s)	221
AccII	CGCG	6 cut(s)	56, 1077, 1167, 1235, 1275, 1488
AccIII	TCCGGA	1 cut(s)	254
AciI	CCGC	8 cut(s)	24, 33, 36, 167, 221, 517, 1075, 1235
AclWI	GGATC	5 cut(s)	252, 265, 480, 926, 1330
AcoI	YGGCCR	2 cut(s)	588, 1072
AcsI	RAATTY	1 cut(s)	987
AcuI	CTGAAG	1 cut(s)	1355
AfaI	GTAC	3 cut(s)	500, 1343, 1382
AfiI	CCNNNNNNNGG	1 cut(s)	83
AgsI	TTSAA	6 cut(s)	13, 122, 292, 561, 782, 1091
AhdI	GACNNNNNGTC	1 cut(s)	1405
AjnI	CCWGG	1 cut(s)	1135
AluBI	AGCT	9 cut(s)	148, 197, 620, 632, 697, 1015, 1171, 1240, 1339
AluI	AGCT	9 cut(s)	148, 197, 620, 632, 697, 1015, 1171, 1240, 1339
Alw21I	GWGCWC	1 cut(s)	199
Alw26I	GTCTC	3 cut(s)	236, 834, 1391
AlwI	GGATC	5 cut(s)	252, 265, 480, 926, 1330
Aor13HI	TCCGGA	1 cut(s)	254
AoxI	GGCC	5 cut(s)	435, 564, 588, 1072, 1468
ApeKI	GCWGC	5 cut(s)	148, 226, 1157, 1160, 1445
ApoI	RAATTY	1 cut(s)	987
Asp700I	GAANNNNTTC	1 cut(s)	979
AspLEI	GCGC	3 cut(s)	58, 1275, 1490
AspS9I	GGNCC	2 cut(s)	565, 1189
AsuC2I	CCSGG	2 cut(s)	102, 890
AsuHPI	GGTGA	1 cut(s)	884
AvaII	GGWCC	1 cut(s)	1189
BaeGI	GKGCMC	1 cut(s)	136
BaeI	ACNNNNGTAYC	2 cut(s)	234, 267
BalI	TGGCCA	1 cut(s)	590
BamHI	GGATCC	1 cut(s)	257
BanI	GGYRCC	2 cut(s)	855, 1142
BanII	GRGCYC	3 cut(s)	199, 903, 1379
BarI	GAAGNNNNNNTAC	2 cut(s)	1364, 1396
BbsI	GAAGAC	1 cut(s)	891
Bbv12I	GWGCWC	1 cut(s)	199
BbvI	GCAGC	5 cut(s)	135, 213, 1144, 1147, 1432
BccI	CCATC	2 cut(s)	413, 1154
BceAI	ACGGC	3 cut(s)	402, 859, 1101
BciT130I	CCWGG	1 cut(s)	1137
BciVI	GTATCC	1 cut(s)	262
BclI	TGATCA	3 cut(s)	658, 1036, 1432
BcnI	CCSGG	2 cut(s)	102, 890
BcoDI	GTCTC	3 cut(s)	236, 834, 1391
BfaI	CTAG	3 cut(s)	305, 1100, 1220
BfmI	CTRYAG	4 cut(s)	149, 310, 627, 1390
BfuAI	ACCTGC	1 cut(s)	1245
BfuI	GTATCC	1 cut(s)	262
BglI	GCCNNNNNGGC	1 cut(s)	449
BisI	GCNGC	7 cut(s)	36, 149, 227, 1075, 1158, 1161, 1446
BlsI	GCNGC	7 cut(s)	37, 150, 228, 1076, 1159, 1162, 1447
Bme1390I	CCNGG	3 cut(s)	102, 890, 1137
Bme18I	GGWCC	1 cut(s)	1189
BmeRI	GACNNNNNGTC	1 cut(s)	1405
BmgT120I	GGNCC	2 cut(s)	565, 1189
BmiI	GGNNCC	3 cut(s)	259, 857, 1144
BmrFI	CCNGG	3 cut(s)	102, 890, 1137
BmrI	ACTGGG	2 cut(s)	654, 806
BmsI	GCATC	6 cut(s)	613, 629, 763, 931, 1034, 1432
BmuI	ACTGGG	2 cut(s)	654, 806
BpiI	GAAGAC	1 cut(s)	891
BplI	GAGNNNNNCTC	2 cut(s)	1289, 1321
BpmI	CTGGAG	2 cut(s)	1158, 1193
Bpu10I	CCTNAGC	2 cut(s)	582, 957
BpuMI	CCSGG	2 cut(s)	102, 890
BsaJI	CCNNGG	6 cut(s)	101, 344, 591, 740, 1117, 1281
BsaWI	WCCGGW	2 cut(s)	254, 1319
Bsc4I	CCNNNNNNNGG	1 cut(s)	83
Bse118I	RCCGGY	2 cut(s)	1021, 1095
Bse1I	ACTGG	6 cut(s)	174, 444, 649, 812, 992, 1191
Bse3DI	GCAATG	2 cut(s)	187, 1497
BseAI	TCCGGA	1 cut(s)	254
BseBI	CCWGG	1 cut(s)	1137
BseDI	CCNNGG	6 cut(s)	101, 344, 591, 740, 1117, 1281
BseGI	GGATG	4 cut(s)	424, 644, 946, 1146
BseLI	CCNNNNNNNGG	1 cut(s)	83
BseMI	GCAATG	2 cut(s)	187, 1497
BseMII	CTCAG	2 cut(s)	470, 948
BseNI	ACTGG	6 cut(s)	174, 444, 649, 812, 992, 1191
BseRI	GAGGAG	1 cut(s)	497
BseSI	GKGCMC	1 cut(s)	136
BseXI	GCAGC	5 cut(s)	135, 213, 1144, 1147, 1432
BseYI	CCCAGC	1 cut(s)	1448
BsgI	GTGCAG	1 cut(s)	1146
Bsh1236I	CGCG	6 cut(s)	56, 1077, 1167, 1235, 1275, 1488
BshFI	GGCC	5 cut(s)	437, 566, 590, 1074, 1470
BshNI	GGYRCC	2 cut(s)	855, 1142
BsiHKAI	GWGCWC	1 cut(s)	199
BsiSI	CCGG	7 cut(s)	101, 255, 890, 1022, 1096, 1320, 1369
BslFI	GGGAC	3 cut(s)	326, 568, 794
BslI	CCNNNNNNNGG	1 cut(s)	83
BsmAI	GTCTC	3 cut(s)	236, 834, 1391
BsmBI	CGTCTC	1 cut(s)	834
BsmFI	GGGAC	3 cut(s)	326, 568, 794
BsmI	GAATGC	1 cut(s)	163
BsnI	GGCC	5 cut(s)	437, 566, 590, 1074, 1470
Bsp1286I	GDGCHC	4 cut(s)	136, 199, 903, 1379
Bsp13I	TCCGGA	1 cut(s)	254
Bsp1407I	TGTACA	1 cut(s)	1341
Bsp143I	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
Bsp19I	CCATGG	2 cut(s)	344, 591
BspACI	CCGC	8 cut(s)	24, 33, 36, 167, 221, 517, 1075, 1235
BspANI	GGCC	5 cut(s)	437, 566, 590, 1074, 1470
BspCNI	CTCAG	2 cut(s)	469, 949
BspEI	TCCGGA	1 cut(s)	254
BspFNI	CGCG	6 cut(s)	56, 1077, 1167, 1235, 1275, 1488
BspHI	TCATGA	1 cut(s)	865
BspLI	GGNNCC	3 cut(s)	259, 857, 1144
BspMAI	CTGCAG	1 cut(s)	153
BspMI	ACCTGC	1 cut(s)	1245
BspPI	GGATC	5 cut(s)	252, 265, 480, 926, 1330
BspQI	GCTCTTC	2 cut(s)	1106, 1367
BspT107I	GGYRCC	2 cut(s)	855, 1142
BsrBI	CCGCTC	1 cut(s)	221
BsrDI	GCAATG	2 cut(s)	187, 1497
BsrFI	RCCGGY	2 cut(s)	1021, 1095
BsrGI	TGTACA	1 cut(s)	1341
BsrI	ACTGG	6 cut(s)	174, 444, 649, 812, 992, 1191
BssAI	RCCGGY	2 cut(s)	1021, 1095
BssECI	CCNNGG	6 cut(s)	101, 344, 591, 740, 1117, 1281
BssMI	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
BssT1I	CCWWGG	4 cut(s)	344, 591, 740, 1281
Bst2UI	CCWGG	1 cut(s)	1137
Bst4CI	ACNGT	4 cut(s)	239, 928, 1360, 1385
Bst6I	CTCTTC	3 cut(s)	1106, 1157, 1367
BstAPI	GCANNNNNTGC	1 cut(s)	802
BstAUI	TGTACA	1 cut(s)	1341
BstC8I	GCNNGC	8 cut(s)	28, 515, 519, 667, 1023, 1165, 1169, 1252
BstDEI	CTNAG	6 cut(s)	322, 456, 582, 789, 860, 957
BstDSI	CCRYGG	3 cut(s)	344, 591, 1117
BstEII	GGTNACC	1 cut(s)	169
BstF5I	GGATG	4 cut(s)	424, 644, 946, 1146
BstFNI	CGCG	6 cut(s)	56, 1077, 1167, 1235, 1275, 1488
BstHHI	GCGC	3 cut(s)	58, 1275, 1490
BstKTI	GATC	7 cut(s)	260, 475, 661, 934, 1039, 1325, 1435
BstMAI	GTCTC	3 cut(s)	236, 834, 1391
BstMBI	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
BstMWI	GCNNNNNNNGC	7 cut(s)	32, 449, 587, 802, 843, 1374, 1445
BstNI	CCWGG	1 cut(s)	1137
BstNSI	RCATGY	2 cut(s)	669, 965
BstPI	GGTNACC	1 cut(s)	169
BstSCI	CCNGG	3 cut(s)	100, 888, 1135
BstSFI	CTRYAG	4 cut(s)	149, 310, 627, 1390
BstSLI	GKGCMC	1 cut(s)	136
BstUI	CGCG	6 cut(s)	56, 1077, 1167, 1235, 1275, 1488
BstV1I	GCAGC	5 cut(s)	135, 213, 1144, 1147, 1432
BstV2I	GAAGAC	1 cut(s)	891
BstX2I	RGATCY	2 cut(s)	257, 472
BstYI	RGATCY	2 cut(s)	257, 472
BsuI	GTATCC	1 cut(s)	262
BsuRI	GGCC	5 cut(s)	437, 566, 590, 1074, 1470
BtgI	CCRYGG	3 cut(s)	344, 591, 1117
BtsCI	GGATG	4 cut(s)	424, 644, 946, 1146
BtsI	GCAGTG	1 cut(s)	1268
BtsIMutI	CAGTG	7 cut(s)	181, 244, 451, 642, 819, 1184, 1268
BveI	ACCTGC	1 cut(s)	1245
Cac8I	GCNNGC	8 cut(s)	28, 515, 519, 667, 1023, 1165, 1169, 1252
CciI	TCATGA	1 cut(s)	865
CfoI	GCGC	3 cut(s)	58, 1275, 1490
Cfr10I	RCCGGY	2 cut(s)	1021, 1095
Cfr13I	GGNCC	2 cut(s)	565, 1189
Csp6I	GTAC	3 cut(s)	499, 1342, 1381
CspCI	CAANNNNNGTGG	2 cut(s)	427, 462
CviQI	GTAC	3 cut(s)	499, 1342, 1381
DdeI	CTNAG	6 cut(s)	322, 456, 582, 789, 860, 957
DpnI	GATC	7 cut(s)	259, 474, 660, 933, 1038, 1324, 1434
DpnII	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
DrdI	GACNNNNNNGTC	1 cut(s)	332
DriI	GACNNNNNGTC	1 cut(s)	1405
DseDI	GACNNNNNNGTC	1 cut(s)	332
EaeI	YGGCCR	2 cut(s)	588, 1072
Eam1104I	CTCTTC	3 cut(s)	1106, 1157, 1367
Eam1105I	GACNNNNNGTC	1 cut(s)	1405
EarI	CTCTTC	3 cut(s)	1106, 1157, 1367
EciI	GGCGGA	2 cut(s)	13, 22
Ecl136II	GAGCTC	1 cut(s)	197
Eco130I	CCWWGG	4 cut(s)	344, 591, 740, 1281
Eco24I	GRGCYC	3 cut(s)	199, 903, 1379
Eco47I	GGWCC	1 cut(s)	1189
Eco53kI	GAGCTC	1 cut(s)	197
Eco57I	CTGAAG	1 cut(s)	1355
Eco91I	GGTNACC	1 cut(s)	169
EcoICRI	GAGCTC	1 cut(s)	197
EcoO65I	GGTNACC	1 cut(s)	169
EcoRI	GAATTC	1 cut(s)	987
EcoRII	CCWGG	1 cut(s)	1135
EcoT14I	CCWWGG	4 cut(s)	344, 591, 740, 1281
EcoT22I	ATGCAT	1 cut(s)	163
EcoT38I	GRGCYC	3 cut(s)	199, 903, 1379
ErhI	CCWWGG	4 cut(s)	344, 591, 740, 1281
Esp3I	CGTCTC	1 cut(s)	834
FaqI	GGGAC	3 cut(s)	326, 568, 794
FauI	CCCGC	1 cut(s)	510
FbaI	TGATCA	3 cut(s)	658, 1036, 1432
Fnu4HI	GCNGC	7 cut(s)	36, 149, 227, 1075, 1158, 1161, 1446
FokI	GGATG	4 cut(s)	431, 651, 953, 1133
FriOI	GRGCYC	3 cut(s)	199, 903, 1379
Fsp4HI	GCNGC	7 cut(s)	36, 149, 227, 1075, 1158, 1161, 1446
FspBI	CTAG	3 cut(s)	305, 1100, 1220
GlaI	GCGC	3 cut(s)	57, 1274, 1489
GluI	GCNGC	7 cut(s)	36, 149, 227, 1075, 1158, 1161, 1446
GsaI	CCCAGC	1 cut(s)	1452
GsuI	CTGGAG	2 cut(s)	1158, 1193
HaeIII	GGCC	5 cut(s)	437, 566, 590, 1074, 1470
HapII	CCGG	7 cut(s)	101, 255, 890, 1022, 1096, 1320, 1369
HhaI	GCGC	3 cut(s)	58, 1275, 1490
Hin6I	GCGC	3 cut(s)	56, 1273, 1488
HinP1I	GCGC	3 cut(s)	56, 1273, 1488
HincII	GTYRAC	2 cut(s)	924, 1081
HindII	GTYRAC	2 cut(s)	924, 1081
HinfI	GANTC	8 cut(s)	68, 975, 1122, 1175, 1214, 1277, 1286, 1499
HpaI	GTTAAC	1 cut(s)	924
HpaII	CCGG	7 cut(s)	101, 255, 890, 1022, 1096, 1320, 1369
HphI	GGTGA	1 cut(s)	884
Hpy166II	GTNNAC	2 cut(s)	924, 1081
Hpy188I	TCNGA	3 cut(s)	681, 1050, 1335
Hpy188III	TCNNGA	7 cut(s)	122, 255, 425, 866, 935, 984, 1503
Hpy8I	GTNNAC	2 cut(s)	924, 1081
Hpy99I	CGWCG	1 cut(s)	1411
HpyAV	CCTTC	6 cut(s)	259, 463, 680, 901, 1003, 1481
HpyCH4III	ACNGT	4 cut(s)	239, 928, 1360, 1385
HpyCH4V	TGCA	9 cut(s)	151, 161, 527, 605, 642, 944, 1163, 1204, 1250
HpyF10VI	GCNNNNNNNGC	7 cut(s)	32, 449, 587, 802, 843, 1374, 1445
HpyF3I	CTNAG	6 cut(s)	322, 456, 582, 789, 860, 957
HspAI	GCGC	3 cut(s)	56, 1273, 1488
Kpn2I	TCCGGA	1 cut(s)	254
KroI	GCCGGC	1 cut(s)	1021
KroNI	GCCGGC	1 cut(s)	1023
Ksp22I	TGATCA	3 cut(s)	658, 1036, 1432
KspAI	GTTAAC	1 cut(s)	924
Kzo9I	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
LguI	GCTCTTC	2 cut(s)	1106, 1367
LmnI	GCTCC	5 cut(s)	35, 617, 742, 1012, 1237
Lsp1109I	GCAGC	5 cut(s)	135, 213, 1144, 1147, 1432
LweI	GCATC	6 cut(s)	613, 629, 763, 931, 1034, 1432
MaeI	CTAG	3 cut(s)	305, 1100, 1220
MaeIII	GTNAC	5 cut(s)	169, 814, 1327, 1354, 1360
MalI	GATC	7 cut(s)	259, 474, 660, 933, 1038, 1324, 1434
MbiI	CCGCTC	1 cut(s)	221
MboI	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
MboII	GAAGA	6 cut(s)	105, 782, 896, 1123, 1144, 1384
MfeI	CAATTG	1 cut(s)	8
MflI	RGATCY	2 cut(s)	257, 472
MhlI	GDGCHC	4 cut(s)	136, 199, 903, 1379
MlsI	TGGCCA	1 cut(s)	590
MluNI	TGGCCA	1 cut(s)	590
MlyI	GAGTC	5 cut(s)	1131, 1184, 1208, 1286, 1493
MmeI	TCCRAC	1 cut(s)	595
Mox20I	TGGCCA	1 cut(s)	590
Mph1103I	ATGCAT	1 cut(s)	163
MroI	TCCGGA	1 cut(s)	254
MroNI	GCCGGC	1 cut(s)	1021
MroXI	GAANNNNTTC	1 cut(s)	979
MscI	TGGCCA	1 cut(s)	590
MseI	TTAA	4 cut(s)	350, 923, 1181, 1209
MslI	CAYNNNNRTG	1 cut(s)	870
Msp20I	TGGCCA	1 cut(s)	590
MspI	CCGG	7 cut(s)	101, 255, 890, 1022, 1096, 1320, 1369
MspR9I	CCNGG	3 cut(s)	102, 890, 1137
MunI	CAATTG	1 cut(s)	8
Mva1269I	GAATGC	1 cut(s)	163
MvaI	CCWGG	1 cut(s)	1137
MvnI	CGCG	6 cut(s)	56, 1077, 1167, 1235, 1275, 1488
MwoI	GCNNNNNNNGC	7 cut(s)	32, 449, 587, 802, 843, 1374, 1445
NaeI	GCCGGC	1 cut(s)	1023
NciI	CCSGG	2 cut(s)	102, 890
NcoI	CCATGG	2 cut(s)	344, 591
NdeII	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
NgoMIV	GCCGGC	1 cut(s)	1021
NlaIV	GGNNCC	3 cut(s)	259, 857, 1144
NmuCI	GTSAC	2 cut(s)	814, 1360
NsiI	ATGCAT	1 cut(s)	163
NspI	RCATGY	2 cut(s)	669, 965
PaeI	GCATGC	1 cut(s)	669
PagI	TCATGA	1 cut(s)	865
PaqCI	CACCTGC	1 cut(s)	1245
PciSI	GCTCTTC	2 cut(s)	1106, 1367
PctI	GAATGC	1 cut(s)	163
PdiI	GCCGGC	1 cut(s)	1023
PdmI	GAANNNNTTC	1 cut(s)	979
PfeI	GAWTC	3 cut(s)	68, 975, 1286
PfoI	TCCNGGA	1 cut(s)	1135
PkrI	GCNGC	7 cut(s)	37, 150, 228, 1076, 1159, 1162, 1447
PleI	GAGTC	5 cut(s)	1130, 1183, 1208, 1285, 1493
PpsI	GAGTC	5 cut(s)	1130, 1183, 1208, 1285, 1493
Psp124BI	GAGCTC	1 cut(s)	199
Psp6I	CCWGG	1 cut(s)	1135
PspEI	GGTNACC	1 cut(s)	169
PspFI	CCCAGC	1 cut(s)	1448
PspGI	CCWGG	1 cut(s)	1135
PspN4I	GGNNCC	3 cut(s)	259, 857, 1144
PspPI	GGNCC	2 cut(s)	565, 1189
PstI	CTGCAG	1 cut(s)	153
PsuI	RGATCY	2 cut(s)	257, 472
RsaI	GTAC	3 cut(s)	500, 1343, 1382
RsaNI	GTAC	3 cut(s)	499, 1342, 1381
RseI	CAYNNNNRTG	1 cut(s)	870
SacI	GAGCTC	1 cut(s)	199
SapI	GCTCTTC	2 cut(s)	1106, 1367
SaqAI	TTAA	4 cut(s)	350, 923, 1181, 1209
SatI	GCNGC	7 cut(s)	36, 149, 227, 1075, 1158, 1161, 1446
Sau3AI	GATC	7 cut(s)	257, 472, 658, 931, 1036, 1322, 1432
Sau96I	GGNCC	2 cut(s)	565, 1189
SchI	GAGTC	5 cut(s)	1131, 1184, 1208, 1286, 1493
ScrFI	CCNGG	3 cut(s)	102, 890, 1137
SduI	GDGCHC	4 cut(s)	136, 199, 903, 1379
SfaNI	GCATC	6 cut(s)	613, 629, 763, 931, 1034, 1432
SfcI	CTRYAG	4 cut(s)	149, 310, 627, 1390
SinI	GGWCC	1 cut(s)	1189
SmiMI	CAYNNNNRTG	1 cut(s)	870
SphI	GCATGC	1 cut(s)	669
SsiI	CCGC	8 cut(s)	24, 33, 36, 167, 221, 517, 1075, 1235
SspMI	CTAG	3 cut(s)	305, 1100, 1220
SstI	GAGCTC	1 cut(s)	199
StyD4I	CCNGG	3 cut(s)	100, 888, 1135
StyI	CCWWGG	4 cut(s)	344, 591, 740, 1281
TaaI	ACNGT	4 cut(s)	239, 928, 1360, 1385
TaqI	TCGA	1 cut(s)	1497
TaqII	GACCGA	1 cut(s)	1206
TatI	WGTACW	2 cut(s)	498, 1341
TauI	GCSGC	2 cut(s)	38, 1077
TfiI	GAWTC	3 cut(s)	68, 975, 1286
Tru1I	TTAA	4 cut(s)	350, 923, 1181, 1209
Tru9I	TTAA	4 cut(s)	350, 923, 1181, 1209
TscAI	CASTG	7 cut(s)	181, 244, 451, 649, 819, 1191, 1275
TseFI	GTSAC	2 cut(s)	814, 1360
TseI	GCWGC	5 cut(s)	148, 226, 1157, 1160, 1445
Tsp45I	GTSAC	2 cut(s)	814, 1360
TspDTI	ATGAA	6 cut(s)	105, 907, 1124, 1143, 1282, 1444
TspGWI	ACGGA	1 cut(s)	66
TspRI	CASTG	7 cut(s)	181, 244, 451, 649, 819, 1191, 1275
VpaK11BI	GGWCC	1 cut(s)	1189
XapI	RAATTY	1 cut(s)	987
XceI	RCATGY	2 cut(s)	669, 965
XmnI	GAANNNNTTC	1 cut(s)	979
XspI	CTAG	3 cut(s)	305, 1100, 1220
Zsp2I	ATGCAT	1 cut(s)	163

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.