Basic Information

Type: Sequence Only

Biological Identity

Species

rosa_samantha

Chromosome

Unknown

Orthogroup ID

OG0004712

Physical Location & Seq

Genomic Coordinates Reverse (-)

0 .. 0

Gene Structure 1 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG115900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGGCTCAGTTGAATACCATGTCCGCTTGCTTGCCTACCAACTTCGCCTTTCACGCTCCAAAATCAGAACTGCCCAGTTACAGTGAGACTGAAGTTGCTGGATCTACAAAGGAAAGAGGATTACGTGGCAAATTGAACAAGGTTGTTCTGGCCTATAGTGGTGGCTTAGACACGTCAGTCATTGTCCCATGGCTAAGGGAGAACTATGGTTGTGAAGTTGTTTGCTTCACTGCCGACGTGGGTCAAGGTATAAAAGAATTGGATGGTCTGGAACAGAAGGCCAAGGCGAGTGGGGCTTGTCAGTTAGTAGTGAAGGATCTGAAGGAGGAGTTTGTAAAAGACTATGTATTCCCTTGCTTGCGAGCTGGTGCAATCTATGAGAGGAAATACTTGCTGGGGACCTCAATGGCTCGTCCTGTTATTGCTAAGGCCATGGTAGATGTTGCCAAGGCAGTTGGAGCTGATGCCGTCGCTCATGGATGCACAGGGAAAGGAAATGATCAGGTTCGGTTTGAGCTGACGTTTTTTGCTCTCGACCCCAAACTTAATGTGGTGGCTCCTTGGAGGGAATGGGACATTACGGGTAGAGAAGATGCTATTGAATATGCTAAGAAGCATAATGTGCCTGTCCCAGTGACAAAGAAATCCATATACAGCAGAGATGGGAACTTATGGCACCTTAGTCATGAGGGTGATATTTTGGAAGACCCGGAAAATGAGCCGAAGAAGGATATGTTCATGTTATCTGTCGATCCAGAAGATGCACCGAATGAACCTGAGTATGTAGATATCGGGATCGTTTCAGGAATTCCAGTTTCAGTCGATGGACAAGAGCTTTCACCAGCGTCTTTGCTTACGAAGCTTAATGAGATTGGCGGAAGACACGGAATTGGTCGTATTGACATGGTTGAAAACCGTCTTGTTGGTATGAAGAGCCGTGGAGTCTATGAAACTCCTGGAGGCACCATCCTATTCGCTGCTGCACGAGAGTTGGAGTCCTTAACTTTGGATCGAGAAACAATGCAAGTTAAAGACTCGCTTGCCCTCAAATATGCAGAGCTAGTCTATGCAGGCAGGTGGTTCGACCCACTTCGTGAGTCCATGGATGCATTCATGGAGAAAATCTCGGAGACAACAACGGGATCAGTAACTCTAAAGTTGTACAAGGGTTCGATCACTGTAACTAGCCGTAAGAGTCCATATAGCCTGTATAGGCAAGACATCTCTTCATTTGAGAGCGGAGATGTGTATGATCAAGCCGATGCAGCTGGGTTTATTAGGCTATATGGTCTCCCCATGAGGGTGCGGGCAATGCTTAAGGAGGGGATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0000053 GO:0003674 GO:0003824 GO:0004055 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006575 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0019627 GO:0019752 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0071941 GO:0072350 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko01100 ko01110 ko01130 ko01230 ko05418 map00220 map00250 map01100 map01110 map01130 map01230 map05418

Pfam Domains

Protein Families

Arginosuc_synth

Protein Analysis

443

Amino Acids

48.82

Weight (kDa)

5.36

Isoelectric Point (pI)

31.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arginosuc_synth	PF00764	47 - 209	1.1e-68	Arginosuccinate synthase N-terminal HUP domain
Arginosuc_syn_C	PF20979	218 - 435	3e-82	Arginosuccinate synthase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24830 AT4G24830
fragaria_vesca	FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g07910 FvH4_5g30030
malus_domestica	MD06G1144000.v1.1 MD06G1144100.v1.1 MD14G1159300.v1.1
prunus_persica	Prupe.5G153100_v2.0.a1 Prupe.5G153100_v2.0.a1 Prupe.7G058500_v2.0.a1
pyrus_communis	pycom06g13360 pycom14g13300
rosa_chinensis	RchiOBHm_Chr7g0190471 RchiOBHm_Chr7g0190481
rosa_laevigata	RLG00000004524 RLG00000004526
rosa_multiflora	Rmu_sc0000526.1_g000010
rosa_roxburghii	Rroxscaffold_3G00264570
rosa_rugosa	Rorug06G0504800
rosa_samantha	Rh7AG110900 Rh7BG113700 Rh7BG113800 Rh7CG115900 Rh7DG114600 Rh7DG114700
rosa_wichuraiana	Rw0G023270 Rw7G009670 Rw7G009680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1065
AasI	GACNNNNNNGTC	1 cut(s)	176
Acc36I	ACCTGC	1 cut(s)	1065
AccB1I	GGYRCC	2 cut(s)	675, 962
AccBSI	CCGCTC	1 cut(s)	1239
AciI	CCGC	4 cut(s)	24, 876, 1239, 1306
AclWI	GGATC	6 cut(s)	109, 324, 746, 803, 1017, 1150
AcsI	RAATTY	1 cut(s)	807
AcuI	CTGAAG	2 cut(s)	111, 341
AdeI	CACNNNGTG	1 cut(s)	1094
AfaI	GTAC	1 cut(s)	1163
AfiI	CCNNNNNNNGG	1 cut(s)	1300
AflII	CTTAAG	1 cut(s)	1316
AflIII	ACRYGT	1 cut(s)	171
AgsI	TTSAA	4 cut(s)	13, 136, 602, 911
AjiI	CACGTC	2 cut(s)	174, 238
AjnI	CCWGG	1 cut(s)	955
AluBI	AGCT	7 cut(s)	365, 461, 517, 835, 862, 1060, 1268
AluI	AGCT	7 cut(s)	365, 461, 517, 835, 862, 1060, 1268
Alw26I	GTCTC	3 cut(s)	80, 1124, 1295
AlwI	GGATC	6 cut(s)	109, 324, 746, 803, 1017, 1150
AoxI	GGCC	3 cut(s)	150, 279, 429
ApeKI	GCWGC	3 cut(s)	977, 980, 1265
ApoI	RAATTY	1 cut(s)	807
AspS9I	GGNCC	1 cut(s)	399
AsuC2I	CCSGG	1 cut(s)	710
AsuHPI	GGTGA	2 cut(s)	704, 831
AvaII	GGWCC	1 cut(s)	399
BanI	GGYRCC	2 cut(s)	675, 962
BauI	CACGAG	1 cut(s)	984
BbsI	GAAGAC	2 cut(s)	711, 886
BbvI	GCAGC	3 cut(s)	964, 967, 1277
BccI	CCATC	4 cut(s)	257, 656, 818, 974
BceAI	ACGGC	3 cut(s)	452, 921, 1173
BcgI	CGANNNNNNTGC	2 cut(s)	1063, 1097
BciT130I	CCWGG	1 cut(s)	957
BclI	TGATCA	2 cut(s)	499, 1252
BcnI	CCSGG	1 cut(s)	710
BcoDI	GTCTC	3 cut(s)	80, 1124, 1295
BfaI	CTAG	3 cut(s)	1061, 1185, 1330
BfmI	CTRYAG	1 cut(s)	154
BfrI	CTTAAG	1 cut(s)	1316
BfuAI	ACCTGC	1 cut(s)	1065
BisI	GCNGC	3 cut(s)	978, 981, 1266
BlsI	GCNGC	3 cut(s)	979, 982, 1267
Bme1390I	CCNGG	2 cut(s)	710, 957
Bme18I	GGWCC	1 cut(s)	399
BmgBI	CACGTC	2 cut(s)	174, 238
BmgT120I	GGNCC	1 cut(s)	399
BmiI	GGNNCC	4 cut(s)	400, 558, 677, 964
BmrFI	CCNGG	2 cut(s)	710, 957
BmrI	ACTGGG	2 cut(s)	69, 626
BmsI	GCATC	6 cut(s)	454, 470, 583, 751, 1096, 1252
BmuI	ACTGGG	2 cut(s)	69, 626
BoxI	GACNNNNGTC	1 cut(s)	240
BpiI	GAAGAC	2 cut(s)	711, 886
BplI	GAGNNNNNCTC	2 cut(s)	1109, 1141
BpmI	CTGGAG	1 cut(s)	978
Bpu10I	CCTNAGC	2 cut(s)	194, 426
BpuMI	CCSGG	1 cut(s)	710
BsaAI	YACGTR	1 cut(s)	125
BsaI	GGTCTC	1 cut(s)	1295
BsaJI	CCNNGG	7 cut(s)	188, 282, 432, 447, 560, 937, 1101
Bsc4I	CCNNNNNNNGG	1 cut(s)	1300
Bse1I	ACTGG	3 cut(s)	75, 632, 812
Bse3DI	GCAATG	1 cut(s)	1317
BseBI	CCWGG	1 cut(s)	957
BseDI	CCNNGG	7 cut(s)	188, 282, 432, 447, 560, 937, 1101
BseGI	GGATG	4 cut(s)	268, 485, 966, 1111
BseLI	CCNNNNNNNGG	1 cut(s)	1300
BseMI	GCAATG	1 cut(s)	1317
BseMII	CTCAG	2 cut(s)	20, 768
BseNI	ACTGG	3 cut(s)	75, 632, 812
BseRI	GAGGAG	1 cut(s)	341
BseXI	GCAGC	3 cut(s)	964, 967, 1277
BseYI	CCCAGC	2 cut(s)	394, 1268
BsgI	GTGCAG	1 cut(s)	966
BshFI	GGCC	3 cut(s)	152, 281, 431
BshNI	GGYRCC	2 cut(s)	675, 962
BsiSI	CCGG	1 cut(s)	710
BslFI	GGGAC	4 cut(s)	170, 412, 587, 614
BslI	CCNNNNNNNGG	1 cut(s)	1300
BsmAI	GTCTC	3 cut(s)	80, 1124, 1295
BsmFI	GGGAC	4 cut(s)	170, 412, 587, 614
BsmI	GAATGC	1 cut(s)	1109
BsnI	GGCC	3 cut(s)	152, 281, 431
Bso31I	GGTCTC	1 cut(s)	1295
Bsp1407I	TGTACA	1 cut(s)	1161
Bsp19I	CCATGG	3 cut(s)	188, 432, 1101
BspACI	CCGC	4 cut(s)	24, 876, 1239, 1306
BspANI	GGCC	3 cut(s)	152, 281, 431
BspCNI	CTCAG	2 cut(s)	19, 769
BspHI	TCATGA	1 cut(s)	685
BspLI	GGNNCC	4 cut(s)	400, 558, 677, 964
BspMI	ACCTGC	1 cut(s)	1065
BspPI	GGATC	6 cut(s)	109, 324, 746, 803, 1017, 1150
BspQI	GCTCTTC	1 cut(s)	926
BspT107I	GGYRCC	2 cut(s)	675, 962
BspTI	CTTAAG	1 cut(s)	1316
BspTNI	GGTCTC	1 cut(s)	1295
BsrBI	CCGCTC	1 cut(s)	1239
BsrDI	GCAATG	1 cut(s)	1317
BsrGI	TGTACA	1 cut(s)	1161
BsrI	ACTGG	3 cut(s)	75, 632, 812
BssECI	CCNNGG	7 cut(s)	188, 282, 432, 447, 560, 937, 1101
BssSI	CACGAG	1 cut(s)	984
BssT1I	CCWWGG	6 cut(s)	188, 282, 432, 447, 560, 1101
Bst2BI	CACGAG	1 cut(s)	984
Bst2UI	CCWGG	1 cut(s)	957
Bst4CI	ACNGT	3 cut(s)	83, 917, 1180
Bst6I	CTCTTC	2 cut(s)	926, 1231
BstAFI	CTTAAG	1 cut(s)	1316
BstAPI	GCANNNNNTGC	1 cut(s)	622
BstAUI	TGTACA	1 cut(s)	1161
BstBAI	YACGTR	1 cut(s)	125
BstC8I	GCNNGC	7 cut(s)	28, 32, 359, 363, 1041, 1072, 1308
BstDEI	CTNAG	7 cut(s)	6, 166, 194, 426, 609, 680, 777
BstDSI	CCRYGG	4 cut(s)	188, 432, 937, 1101
BstF5I	GGATG	4 cut(s)	268, 485, 966, 1111
BstMAI	GTCTC	3 cut(s)	80, 1124, 1295
BstMWI	GCNNNNNNNGC	7 cut(s)	53, 293, 458, 622, 859, 1265, 1312
BstNI	CCWGG	1 cut(s)	957
BstPAI	GACNNNNGTC	1 cut(s)	240
BstSCI	CCNGG	2 cut(s)	708, 955
BstSFI	CTRYAG	1 cut(s)	154
BstV1I	GCAGC	3 cut(s)	964, 967, 1277
BstV2I	GAAGAC	2 cut(s)	711, 886
BstX2I	RGATCY	3 cut(s)	101, 316, 1326
BstYI	RGATCY	3 cut(s)	101, 316, 1326
BsuRI	GGCC	3 cut(s)	152, 281, 431
BtgI	CCRYGG	4 cut(s)	188, 432, 937, 1101
BtrI	CACGTC	2 cut(s)	174, 238
BtsCI	GGATG	4 cut(s)	268, 485, 966, 1111
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	4 cut(s)	88, 228, 639, 1176
BveI	ACCTGC	1 cut(s)	1065
Cac8I	GCNNGC	7 cut(s)	28, 32, 359, 363, 1041, 1072, 1308
CciI	TCATGA	1 cut(s)	685
Cfr13I	GGNCC	1 cut(s)	399
CseI	GACGC	1 cut(s)	834
Csp6I	GTAC	1 cut(s)	1162
CspCI	CAANNNNNGTGG	2 cut(s)	271, 306
CviQI	GTAC	1 cut(s)	1162
DdeI	CTNAG	7 cut(s)	6, 166, 194, 426, 609, 680, 777
DraIII	CACNNNGTG	1 cut(s)	1094
DrdI	GACNNNNNNGTC	1 cut(s)	176
DseDI	GACNNNNNNGTC	1 cut(s)	176
Eam1104I	CTCTTC	2 cut(s)	926, 1231
EarI	CTCTTC	2 cut(s)	926, 1231
EciI	GGCGGA	1 cut(s)	891
Eco130I	CCWWGG	6 cut(s)	188, 282, 432, 447, 560, 1101
Eco31I	GGTCTC	1 cut(s)	1295
Eco32I	GATATC	1 cut(s)	790
Eco47I	GGWCC	1 cut(s)	399
Eco57I	CTGAAG	2 cut(s)	111, 341
EcoO109I	RGGNCCY	1 cut(s)	399
EcoRI	GAATTC	1 cut(s)	807
EcoRII	CCWGG	1 cut(s)	955
EcoRV	GATATC	1 cut(s)	790
EcoT14I	CCWWGG	6 cut(s)	188, 282, 432, 447, 560, 1101
EcoT22I	ATGCAT	1 cut(s)	1111
ErhI	CCWWGG	6 cut(s)	188, 282, 432, 447, 560, 1101
FalI	AAGNNNNNCTT	2 cut(s)	1023, 1055
FaqI	GGGAC	4 cut(s)	170, 412, 587, 614
FauI	CCCGC	1 cut(s)	1299
FbaI	TGATCA	2 cut(s)	499, 1252
Fnu4HI	GCNGC	3 cut(s)	978, 981, 1266
FokI	GGATG	4 cut(s)	275, 492, 953, 1118
Fsp4HI	GCNGC	3 cut(s)	978, 981, 1266
FspBI	CTAG	3 cut(s)	1061, 1185, 1330
GluI	GCNGC	3 cut(s)	978, 981, 1266
GsaI	CCCAGC	2 cut(s)	398, 1272
GsuI	CTGGAG	1 cut(s)	978
HaeIII	GGCC	3 cut(s)	152, 281, 431
HapII	CCGG	1 cut(s)	710
HgaI	GACGC	1 cut(s)	834
HindIII	AAGCTT	1 cut(s)	860
HinfI	GANTC	5 cut(s)	942, 995, 1034, 1097, 1195
HpaII	CCGG	1 cut(s)	710
HphI	GGTGA	2 cut(s)	704, 831
Hpy188I	TCNGA	3 cut(s)	67, 321, 1129
Hpy188III	TCNNGA	8 cut(s)	269, 533, 686, 755, 793, 804, 1013, 1094
Hpy99I	CGWCG	2 cut(s)	239, 473
HpyAV	CCTTC	4 cut(s)	271, 307, 316, 721
HpyCH4III	ACNGT	3 cut(s)	83, 917, 1180
HpyCH4IV	ACGT	4 cut(s)	124, 173, 237, 521
HpyCH4V	TGCA	9 cut(s)	371, 483, 764, 983, 1024, 1055, 1070, 1109, 1265
HpyF10VI	GCNNNNNNNGC	7 cut(s)	53, 293, 458, 622, 859, 1265, 1312
HpyF3I	CTNAG	7 cut(s)	6, 166, 194, 426, 609, 680, 777
HpySE526I	ACGT	4 cut(s)	124, 173, 237, 521
Ksp22I	TGATCA	2 cut(s)	499, 1252
LguI	GCTCTTC	1 cut(s)	926
LmnI	GCTCC	3 cut(s)	61, 458, 562
Lsp1109I	GCAGC	3 cut(s)	964, 967, 1277
LweI	GCATC	6 cut(s)	454, 470, 583, 751, 1096, 1252
MaeI	CTAG	3 cut(s)	1061, 1185, 1330
MaeII	ACGT	4 cut(s)	124, 173, 237, 521
MaeIII	GTNAC	4 cut(s)	77, 634, 1147, 1180
MbiI	CCGCTC	1 cut(s)	1239
MboII	GAAGA	7 cut(s)	602, 716, 736, 770, 891, 943, 1218
MflI	RGATCY	3 cut(s)	101, 316, 1326
MluCI	AATT	4 cut(s)	131, 257, 807, 888
MlyI	GAGTC	5 cut(s)	951, 1004, 1028, 1106, 1204
MmeI	TCCRAC	2 cut(s)	436, 972
Mph1103I	ATGCAT	1 cut(s)	1111
MseI	TTAA	5 cut(s)	546, 864, 1001, 1029, 1317
MslI	CAYNNNNRTG	2 cut(s)	690, 1301
MspA1I	CMGCKG	1 cut(s)	1268
MspCI	CTTAAG	1 cut(s)	1316
MspI	CCGG	1 cut(s)	710
MspR9I	CCNGG	2 cut(s)	710, 957
Mva1269I	GAATGC	1 cut(s)	1109
MvaI	CCWGG	1 cut(s)	957
MwoI	GCNNNNNNNGC	7 cut(s)	53, 293, 458, 622, 859, 1265, 1312
NciI	CCSGG	1 cut(s)	710
NcoI	CCATGG	3 cut(s)	188, 432, 1101
NlaIV	GGNNCC	4 cut(s)	400, 558, 677, 964
NmuCI	GTSAC	1 cut(s)	634
NsiI	ATGCAT	1 cut(s)	1111
PagI	TCATGA	1 cut(s)	685
PaqCI	CACCTGC	1 cut(s)	1065
PciSI	GCTCTTC	1 cut(s)	926
PctI	GAATGC	1 cut(s)	1109
PfoI	TCCNGGA	1 cut(s)	955
PkrI	GCNGC	3 cut(s)	979, 982, 1267
PleI	GAGTC	5 cut(s)	950, 1003, 1028, 1105, 1203
PpsI	GAGTC	5 cut(s)	950, 1003, 1028, 1105, 1203
Ppu21I	YACGTR	1 cut(s)	125
PpuMI	RGGWCCY	1 cut(s)	399
PshAI	GACNNNNGTC	1 cut(s)	240
Psp5II	RGGWCCY	1 cut(s)	399
Psp6I	CCWGG	1 cut(s)	955
PspFI	CCCAGC	2 cut(s)	394, 1268
PspGI	CCWGG	1 cut(s)	955
PspN4I	GGNNCC	4 cut(s)	400, 558, 677, 964
PspPI	GGNCC	1 cut(s)	399
PspPPI	RGGWCCY	1 cut(s)	399
PsuI	RGATCY	3 cut(s)	101, 316, 1326
PvuII	CAGCTG	1 cut(s)	1268
RsaI	GTAC	1 cut(s)	1163
RsaNI	GTAC	1 cut(s)	1162
RseI	CAYNNNNRTG	2 cut(s)	690, 1301
SapI	GCTCTTC	1 cut(s)	926
SaqAI	TTAA	5 cut(s)	546, 864, 1001, 1029, 1317
SatI	GCNGC	3 cut(s)	978, 981, 1266
Sau96I	GGNCC	1 cut(s)	399
SchI	GAGTC	5 cut(s)	951, 1004, 1028, 1106, 1204
ScrFI	CCNGG	2 cut(s)	710, 957
SfaNI	GCATC	6 cut(s)	454, 470, 583, 751, 1096, 1252
SfcI	CTRYAG	1 cut(s)	154
SinI	GGWCC	1 cut(s)	399
SmiMI	CAYNNNNRTG	2 cut(s)	690, 1301
SmlI	CTYRAG	1 cut(s)	1316
SmoI	CTYRAG	1 cut(s)	1316
Sse9I	AATT	4 cut(s)	131, 257, 807, 888
SsiI	CCGC	4 cut(s)	24, 876, 1239, 1306
SspMI	CTAG	3 cut(s)	1061, 1185, 1330
StyD4I	CCNGG	2 cut(s)	708, 955
StyI	CCWWGG	6 cut(s)	188, 282, 432, 447, 560, 1101
TaaI	ACNGT	3 cut(s)	83, 917, 1180
TaiI	ACGT	4 cut(s)	127, 176, 240, 524
TaqI	TCGA	6 cut(s)	534, 750, 822, 1012, 1083, 1172
TasI	AATT	4 cut(s)	131, 257, 807, 888
TatI	WGTACW	1 cut(s)	1161
Tru1I	TTAA	5 cut(s)	546, 864, 1001, 1029, 1317
Tru9I	TTAA	5 cut(s)	546, 864, 1001, 1029, 1317
TscAI	CASTG	4 cut(s)	88, 235, 639, 1183
TseFI	GTSAC	1 cut(s)	634
TseI	GCWGC	3 cut(s)	977, 980, 1265
Tsp45I	GTSAC	1 cut(s)	634
TspDTI	ATGAA	6 cut(s)	727, 786, 944, 963, 1102, 1218
TspGWI	ACGGA	1 cut(s)	900
TspRI	CASTG	4 cut(s)	88, 235, 639, 1183
Vha464I	CTTAAG	1 cut(s)	1316
VpaK11BI	GGWCC	1 cut(s)	399
XapI	RAATTY	1 cut(s)	807
XcmI	CCANNNNNNNNNTGG	1 cut(s)	547
XspI	CTAG	3 cut(s)	1061, 1185, 1330
Zsp2I	ATGCAT	1 cut(s)	1111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.