Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0008045

Physical Location & Seq

Genomic Coordinates Forward (+)

30148810 .. 30155123

Gene Structure 6314 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1160100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGGAGAACGTAAACGAGAGTACAAGCGTTCGAGTTCTATGCCCTAAGCTCGTCCTTGACAAGAACGAACCGGGCCTCCAATGGCTGATTGGATCCCCATTTTTCCCGCCACACACCGTCGTCTCCACCGTCAGATGCATCCACACCGATTCCTCCTCTCCCGACTACCGCAGAGAATCAGAAGAGCTTCGGACATTACTGTTGAAAGGATTCGAGGTGATCGGAGCATTGGTCGTTGCAAATTCCGGTGATGGAATGAGCGCCGCCGGCGAGGCAATTGCTGCGGCACGAAGGCTGCGGAAGCTGCTCCGCAGAGAAAATGGAAAAAAATTGGATAGTCGACAAGTAATTGGTGGTGTTGCGGATTGTAGTGGCGGTGACATTCAGTTTTTCGTGTCGAAATCCGAGAGCTTGACCAGCTTTGAAGCTGTGAATGTGTTGTATGATGGTCATCCTGAAAAGTATGTTTGGGAGAGGGGCTGTCTGCTCCGGTGTGAGCTTCCGTTTAAGCTGCCAATCTATTATCCAGTTAACAAGCCAAAGGATTCAGAAAAGATGTTTCGGCATGCAACTGAAGCAGTTATTGCAAAATTCAAAGATCCAAAGGCTGCGTACTTGGTAGAAGCATTAAGCAAAACATCTGCCGAAGTACCCCAACCTGTCATCCTCCGCGGTGTAGATTTGGATTTTGACACAGATCTCTCAAATGTCAAACTTGCGGGAGAAAGCGCCCAAGATTCTGAGGCTGGTCTTTTGTCGTGTTCCCACTTCTGTTTAGAAAGTAAAAAAGGTGCACCGGTATATTCTGTTGAGCATGCAGATAGAATCCAAGTAAGCATTCTGCTGAATAGTTCGGATAAGTCAGAAAAATCTACGGCACCCGTTGCAGAATATTTTCCAGCTTTGGAAGAAGCCAAAGTTTTGGTGGTCGATTTTAAGCTAGAAGTGCTCTGCTATTCAGCCAAGGGTCTCCCACTGAAGCATGCAGTTTCAAAGTTATTGATCCCGGGGTTGATTGATCAGTTTAATTTAGTGGGAAATACAGTCCTACCCAACCTCTTAGCACAACATCCTCAGCTACATCCATATCACTTTAGCCCTCCTGGTGTTCTGCATCCGATCACTGTTGTCTATGAACTCAACTATGGGGAAACAGAAATGAAGCAAGTTGAAGTTAGAAAATCTTTACACTTAAGACTGGGTTTACCTTTTGATCGTCCTCTTCTAAGAATTGCCAACGCTCTCGATATCTCTACATCGAGGGATGTTGTAAGGAGTGATGCAAAGTGGAAAGGTTCTAGTTTGCTCAAAGATGTACACGTTGGGATTCCAAGCAGTGGCGTTTCTGGGGGCAGTGTGTCTTTGGTGCAAGGCTCTTATGAATACTACCATTATCTACAAGATGCCTTTAATGATTCGGGTTGGGGTTGTGCTTATCGCTCTCTACAAACGATCATTTCATGGTTCAGACTCCAACACTACACATCCGTACAAGTTCCTTCTCATAGGGAAATACAGCAGGCACTTGTAGAGATTGGTGATAAAGATCCTTCTTTCATCGGGTCACACGAATGGATTGGCGCCATCGAGTTGAGCTTTGTCCTGGACAAGCTATTGGGTGTTAGTTGCAAAGTCATGAATGTCCGATCTGGTGCTGAGCTTCCCGAAAAATGTCGAGAGCTGGCCTTACACTTCGAGACTCAAGGAACACCTATTATGATTGGTGGTGGAGTTCTGGCATATACGCTATTGGGAGTTGATTACAACGAAGCAAGCGGAGATTGCGCGTTCCTAATACTCGATCCTCACTACACAGGCAGTGACGAACACAAGAAAATTGTAAATGGCGGATGGTGCGGGTGGAAAAAGGCTGTTGATAGCAAGGGAAAGAGTTTCTTTTTGCATGATAAGTTCTATAATCTTCTGCTTCCACAGAGGCCTAATATGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0019783 GO:0043170 GO:0044238 GO:0070011 GO:0071567 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C78

Protein Analysis

651

Amino Acids

71.84

Weight (kDa)

6.33

Isoelectric Point (pI)

40.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UfSP2_N	PF20908	329 - 416	1.9e-08	Ubiquitin-fold modifier 1 specific protease 2, N-terminal
Peptidase_C78	PF07910	452 - 642	1.6e-67	Peptidase family C78

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008045)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48380 AT3G48380 AT3G48380
fragaria_vesca	FvH4_5g06921 FvH4_5g06950 FvH4_5g06990 FvH4_5g06990 FvH4_5g06990
malus_domestica	MD06G1160100.v1.1 MD14G1166100.v1.1
prunus_persica	Prupe.5G160400_v2.0.a1
pyrus_communis	pycom14g13880
rosa_chinensis	RchiOBHm_Chr7g0191691
rosa_laevigata	RLG00000004433
rosa_multiflora	Rmu_sc0001123.1_g000012 Rmu_sc0018746.1_g000004
rosa_roxburghii	Rroxscaffold_157G00438510 Rroxscaffold_3G00263640
rosa_rugosa	Rorug06G0511000
rosa_samantha	Rh7AG120200 Rh7BG122300 Rh7CG124900 Rh7DG123100
rosa_wichuraiana	Rw7G010230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	877, 1580
AccB7I	CCANNNNNTGG	1 cut(s)	1337
AccI	GTMKAC	1 cut(s)	340
AccII	CGCG	2 cut(s)	672, 1787
AclWI	GGATC	6 cut(s)	87, 100, 593, 997, 1541, 1796
AcsI	RAATTY	2 cut(s)	241, 590
AcuI	CTGAAG	2 cut(s)	594, 998
AcyI	GRCGYC	1 cut(s)	1581
AfaI	GTAC	5 cut(s)	22, 614, 651, 1317, 1491
AfiI	CCNNNNNNNGG	1 cut(s)	1337
AflII	CTTAAG	1 cut(s)	1192
AflIII	ACRYGT	1 cut(s)	1318
AgeI	ACCGGT	1 cut(s)	796
AgsI	TTSAA	5 cut(s)	205, 425, 595, 993, 1172
AjnI	CCWGG	2 cut(s)	1102, 1602
AjuI	GAANNNNNNNTTGG	2 cut(s)	822, 854
AloI	GAACNNNNNNTCC	2 cut(s)	60, 92
Alw21I	GWGCWC	2 cut(s)	796, 951
Alw26I	GTCTC	3 cut(s)	127, 974, 1691
Alw44I	GTGCAC	1 cut(s)	792
AlwI	GGATC	6 cut(s)	87, 100, 593, 997, 1541, 1796
Ama87I	CYCGRG	1 cut(s)	1006
AoxI	GGCC	3 cut(s)	73, 1683, 1937
ApaLI	GTGCAC	1 cut(s)	792
ApeKI	GCWGC	5 cut(s)	281, 295, 304, 511, 608
ApoI	RAATTY	2 cut(s)	241, 590
AsiGI	ACCGGT	1 cut(s)	796
Asp700I	GAANNNNTTC	3 cut(s)	186, 209, 894
AspLEI	GCGC	4 cut(s)	263, 731, 1583, 1787
AspS9I	GGNCC	1 cut(s)	73
AsuC2I	CCSGG	3 cut(s)	72, 1007, 1008
AsuHPI	GGTGA	4 cut(s)	229, 260, 389, 1550
AvaI	CYCGRG	1 cut(s)	1006
BaeGI	GKGCMC	1 cut(s)	796
BamHI	GGATCC	1 cut(s)	92
BanI	GGYRCC	2 cut(s)	877, 1580
Bbv12I	GWGCWC	2 cut(s)	796, 951
BbvCI	CCTCAGC	1 cut(s)	1074
BbvI	GCAGC	5 cut(s)	268, 282, 291, 498, 595
BccI	CCATC	4 cut(s)	245, 440, 1592, 1845
BceAI	ACGGC	1 cut(s)	891
BcgI	CGANNNNNNTGC	4 cut(s)	21, 55, 260, 294
BciT130I	CCWGG	2 cut(s)	1104, 1604
BclI	TGATCA	1 cut(s)	1018
BcnI	CCSGG	3 cut(s)	72, 1007, 1008
BcoDI	GTCTC	3 cut(s)	127, 974, 1691
BfaI	CTAG	2 cut(s)	941, 1299
BfoI	RGCGCY	3 cut(s)	264, 732, 1584
BfrI	CTTAAG	1 cut(s)	1192
BglI	GCCNNNNNGGC	1 cut(s)	272
BglII	AGATCT	1 cut(s)	697
BisI	GCNGC	7 cut(s)	264, 282, 285, 296, 305, 512, 609
BlpI	GCTNAGC	1 cut(s)	1656
BlsI	GCNGC	7 cut(s)	265, 283, 286, 297, 306, 513, 610
Bme1390I	CCNGG	5 cut(s)	72, 1007, 1008, 1104, 1604
BmeT110I	CYCGRG	1 cut(s)	1006
BmgT120I	GGNCC	1 cut(s)	73
BmiI	GGNNCC	3 cut(s)	94, 879, 1582
BmrFI	CCNGG	5 cut(s)	72, 1007, 1008, 1104, 1604
BmrI	ACTGGG	1 cut(s)	1208
BmsI	GCATC	5 cut(s)	125, 147, 1123, 1270, 1393
BmuI	ACTGGG	1 cut(s)	1208
Bpu10I	CCTNAGC	2 cut(s)	45, 1074
Bpu1102I	GCTNAGC	1 cut(s)	1656
BpuEI	CTTGAG	1 cut(s)	1686
BpuMI	CCSGG	3 cut(s)	72, 1007, 1008
BsaBI	GATNNNNATC	2 cut(s)	450, 1545
BsaHI	GRCGYC	1 cut(s)	1581
BsaI	GGTCTC	1 cut(s)	974
BsaJI	CCNNGG	4 cut(s)	670, 963, 1006, 1007
BsaWI	WCCGGW	3 cut(s)	245, 489, 796
BsaXI	ACNNNNNCTCC	4 cut(s)	60, 90, 216, 246
Bsc4I	CCNNNNNNNGG	1 cut(s)	1337
Bse118I	RCCGGY	2 cut(s)	266, 796
Bse1I	ACTGG	2 cut(s)	527, 1203
Bse8I	GATNNNNATC	2 cut(s)	450, 1545
BseBI	CCWGG	2 cut(s)	1104, 1604
BseDI	CCNNGG	4 cut(s)	670, 963, 1006, 1007
BseGI	GGATG	9 cut(s)	138, 451, 663, 1069, 1081, 1114, 1270, 1484, 1856
BseJI	GATNNNNATC	2 cut(s)	450, 1545
BseLI	CCNNNNNNNGG	1 cut(s)	1337
BseMII	CTCAG	3 cut(s)	732, 1088, 1647
BseNI	ACTGG	2 cut(s)	527, 1203
BseRI	GAGGAG	1 cut(s)	145
BseSI	GKGCMC	1 cut(s)	796
BseXI	GCAGC	5 cut(s)	268, 282, 291, 498, 595
Bsh1236I	CGCG	2 cut(s)	672, 1787
BshFI	GGCC	3 cut(s)	75, 1685, 1939
BshNI	GGYRCC	2 cut(s)	877, 1580
BshTI	ACCGGT	1 cut(s)	796
BsiHKAI	GWGCWC	2 cut(s)	796, 951
BsiHKCI	CYCGRG	1 cut(s)	1006
BsiSI	CCGG	6 cut(s)	71, 246, 267, 490, 797, 1007
BslI	CCNNNNNNNGG	1 cut(s)	1337
BsmAI	GTCTC	3 cut(s)	127, 974, 1691
BsmBI	CGTCTC	1 cut(s)	127
BsmI	GAATGC	1 cut(s)	837
BsnI	GGCC	3 cut(s)	75, 1685, 1939
Bso31I	GGTCTC	1 cut(s)	974
BsoBI	CYCGRG	1 cut(s)	1006
Bsp1286I	GDGCHC	2 cut(s)	796, 951
Bsp1407I	TGTACA	1 cut(s)	1315
Bsp1720I	GCTNAGC	1 cut(s)	1656
BspANI	GGCC	3 cut(s)	75, 1685, 1939
BspCNI	CTCAG	3 cut(s)	733, 1087, 1648
BspFNI	CGCG	2 cut(s)	672, 1787
BspHI	TCATGA	1 cut(s)	1635
BspLI	GGNNCC	3 cut(s)	94, 879, 1582
BspPI	GGATC	6 cut(s)	87, 100, 593, 997, 1541, 1796
BspQI	GCTCTTC	1 cut(s)	177
BspT107I	GGYRCC	2 cut(s)	877, 1580
BspTI	CTTAAG	1 cut(s)	1192
BspTNI	GGTCTC	1 cut(s)	974
BsrFI	RCCGGY	2 cut(s)	266, 796
BsrGI	TGTACA	1 cut(s)	1315
BsrI	ACTGG	2 cut(s)	527, 1203
BssAI	RCCGGY	2 cut(s)	266, 796
BssECI	CCNNGG	4 cut(s)	670, 963, 1006, 1007
BssNI	GRCGYC	1 cut(s)	1581
BssT1I	CCWWGG	1 cut(s)	963
Bst2UI	CCWGG	2 cut(s)	1104, 1604
Bst4CI	ACNGT	5 cut(s)	118, 130, 201, 1045, 1126
Bst6I	CTCTTC	2 cut(s)	177, 1227
BstACI	GRCGYC	1 cut(s)	1581
BstAFI	CTTAAG	1 cut(s)	1192
BstAPI	GCANNNNNTGC	3 cut(s)	281, 584, 884
BstAUI	TGTACA	1 cut(s)	1315
BstC8I	GCNNGC	7 cut(s)	268, 567, 816, 984, 1521, 1683, 1774
BstDEI	CTNAG	6 cut(s)	45, 741, 1060, 1074, 1226, 1656
BstDSI	CCRYGG	1 cut(s)	670
BstF5I	GGATG	9 cut(s)	138, 451, 663, 1069, 1081, 1114, 1270, 1484, 1856
BstFNI	CGCG	2 cut(s)	672, 1787
BstH2I	RGCGCY	3 cut(s)	264, 732, 1584
BstHHI	GCGC	4 cut(s)	263, 731, 1583, 1787
BstMAI	GTCTC	3 cut(s)	127, 974, 1691
BstNI	CCWGG	2 cut(s)	1104, 1604
BstNSI	RCATGY	3 cut(s)	569, 818, 986
BstSCI	CCNGG	5 cut(s)	70, 1005, 1006, 1102, 1602
BstSLI	GKGCMC	1 cut(s)	796
BstUI	CGCG	2 cut(s)	672, 1787
BstV1I	GCAGC	5 cut(s)	268, 282, 291, 498, 595
BstX2I	RGATCY	4 cut(s)	92, 598, 697, 1546
BstXI	CCANNNNNNTGG	1 cut(s)	922
BstYI	RGATCY	4 cut(s)	92, 598, 697, 1546
BsuRI	GGCC	3 cut(s)	75, 1685, 1939
BtgI	CCRYGG	1 cut(s)	670
BtsCI	GGATG	9 cut(s)	138, 451, 663, 1069, 1081, 1114, 1270, 1484, 1856
BtsI	GCAGTG	3 cut(s)	1342, 1360, 1825
BtsIMutI	CAGTG	5 cut(s)	974, 1122, 1342, 1360, 1825
Cac8I	GCNNGC	7 cut(s)	268, 567, 816, 984, 1521, 1683, 1774
CciI	TCATGA	1 cut(s)	1635
CfoI	GCGC	4 cut(s)	263, 731, 1583, 1787
Cfr10I	RCCGGY	2 cut(s)	266, 796
Cfr13I	GGNCC	1 cut(s)	73
Cfr42I	CCGCGG	1 cut(s)	673
Cfr9I	CCCGGG	1 cut(s)	1006
Csp6I	GTAC	5 cut(s)	21, 613, 650, 1316, 1490
CspAI	ACCGGT	1 cut(s)	796
CviAII	CATG	6 cut(s)	566, 815, 983, 1461, 1636, 1904
CviQI	GTAC	5 cut(s)	21, 613, 650, 1316, 1490
DdeI	CTNAG	6 cut(s)	45, 741, 1060, 1074, 1226, 1656
DinI	GGCGCC	1 cut(s)	1582
Eam1104I	CTCTTC	2 cut(s)	177, 1227
EarI	CTCTTC	2 cut(s)	177, 1227
EciI	GGCGGA	1 cut(s)	1863
Eco130I	CCWWGG	1 cut(s)	963
Eco147I	AGGCCT	1 cut(s)	1939
Eco31I	GGTCTC	1 cut(s)	974
Eco32I	GATATC	1 cut(s)	1249
Eco57I	CTGAAG	2 cut(s)	594, 998
Eco88I	CYCGRG	1 cut(s)	1006
EcoRII	CCWGG	2 cut(s)	1102, 1602
EcoRV	GATATC	1 cut(s)	1249
EcoT14I	CCWWGG	1 cut(s)	963
EcoT22I	ATGCAT	1 cut(s)	140
EgeI	GGCGCC	1 cut(s)	1582
EheI	GGCGCC	1 cut(s)	1582
ErhI	CCWWGG	1 cut(s)	963
Esp3I	CGTCTC	1 cut(s)	127
FaeI	CATG	6 cut(s)	569, 818, 986, 1464, 1639, 1907
FalI	AAGNNNNNCTT	2 cut(s)	1880, 1912
FatI	CATG	6 cut(s)	565, 814, 982, 1460, 1635, 1903
FauI	CCCGC	3 cut(s)	114, 712, 1850
FbaI	TGATCA	1 cut(s)	1018
FblI	GTMKAC	1 cut(s)	340
Fnu4HI	GCNGC	7 cut(s)	264, 282, 285, 296, 305, 512, 609
FokI	GGATG	9 cut(s)	125, 438, 650, 1056, 1068, 1101, 1277, 1471, 1863
Fsp4HI	GCNGC	7 cut(s)	264, 282, 285, 296, 305, 512, 609
FspBI	CTAG	2 cut(s)	941, 1299
GlaI	GCGC	4 cut(s)	262, 730, 1582, 1786
GluI	GCNGC	7 cut(s)	264, 282, 285, 296, 305, 512, 609
HaeII	RGCGCY	3 cut(s)	264, 732, 1584
HaeIII	GGCC	3 cut(s)	75, 1685, 1939
HapII	CCGG	6 cut(s)	71, 246, 267, 490, 797, 1007
HhaI	GCGC	4 cut(s)	263, 731, 1583, 1787
Hin1I	GRCGYC	1 cut(s)	1581
Hin1II	CATG	6 cut(s)	569, 818, 986, 1464, 1639, 1907
Hin6I	GCGC	4 cut(s)	261, 729, 1581, 1785
HinP1I	GCGC	4 cut(s)	261, 729, 1581, 1785
HincII	GTYRAC	2 cut(s)	341, 532
HindII	GTYRAC	2 cut(s)	341, 532
HpaI	GTTAAC	1 cut(s)	532
HpaII	CCGG	6 cut(s)	71, 246, 267, 490, 797, 1007
HphI	GGTGA	4 cut(s)	229, 260, 389, 1550
Hpy166II	GTNNAC	6 cut(s)	13, 341, 532, 794, 1205, 1318
Hpy188III	TCNNGA	7 cut(s)	161, 455, 1244, 1636, 1664, 1676, 1696
Hpy8I	GTNNAC	6 cut(s)	13, 341, 532, 794, 1205, 1318
Hpy99I	CGWCG	1 cut(s)	122
HpyAV	CCTTC	3 cut(s)	285, 1509, 1560
HpyCH4III	ACNGT	5 cut(s)	118, 130, 201, 1045, 1126
HpyCH4IV	ACGT	2 cut(s)	9, 1320
HpyF3I	CTNAG	6 cut(s)	45, 741, 1060, 1074, 1226, 1656
HpySE526I	ACGT	2 cut(s)	9, 1320
Hsp92I	GRCGYC	1 cut(s)	1581
Hsp92II	CATG	6 cut(s)	569, 818, 986, 1464, 1639, 1907
HspAI	GCGC	4 cut(s)	261, 729, 1581, 1785
KasI	GGCGCC	1 cut(s)	1580
KroI	GCCGGC	1 cut(s)	266
KroNI	GCCGGC	1 cut(s)	268
Ksp22I	TGATCA	1 cut(s)	1018
KspAI	GTTAAC	1 cut(s)	532
KspI	CCGCGG	1 cut(s)	673
LguI	GCTCTTC	1 cut(s)	177
LmnI	GCTCC	3 cut(s)	224, 312, 492
Lsp1109I	GCAGC	5 cut(s)	268, 282, 291, 498, 595
LweI	GCATC	5 cut(s)	125, 147, 1123, 1270, 1393
MaeI	CTAG	2 cut(s)	941, 1299
MaeII	ACGT	2 cut(s)	9, 1320
MaeIII	GTNAC	3 cut(s)	377, 1563, 1820
MboII	GAAGA	4 cut(s)	194, 920, 1214, 1913
MfeI	CAATTG	1 cut(s)	276
MflI	RGATCY	4 cut(s)	92, 598, 697, 1546
MhlI	GDGCHC	2 cut(s)	796, 951
MluCI	AATT	8 cut(s)	241, 276, 329, 348, 590, 1027, 1230, 1836
Mly113I	GGCGCC	1 cut(s)	1581
MlyI	GAGTC	2 cut(s)	1464, 1693
MmeI	TCCRAC	1 cut(s)	1498
Mph1103I	ATGCAT	1 cut(s)	140
MreI	CGCCGGCG	1 cut(s)	266
MroNI	GCCGGC	1 cut(s)	266
MroXI	GAANNNNTTC	3 cut(s)	186, 209, 894
MseI	TTAA	7 cut(s)	507, 531, 629, 936, 1026, 1193, 1410
MslI	CAYNNNNRTG	1 cut(s)	1570
MspA1I	CMGCKG	1 cut(s)	672
MspCI	CTTAAG	1 cut(s)	1192
MspI	CCGG	6 cut(s)	71, 246, 267, 490, 797, 1007
MspR9I	CCNGG	5 cut(s)	72, 1007, 1008, 1104, 1604
MunI	CAATTG	1 cut(s)	276
Mva1269I	GAATGC	1 cut(s)	837
MvaI	CCWGG	2 cut(s)	1104, 1604
MvnI	CGCG	2 cut(s)	672, 1787
NaeI	GCCGGC	1 cut(s)	268
NarI	GGCGCC	1 cut(s)	1581
NciI	CCSGG	3 cut(s)	72, 1007, 1008
NgoMIV	GCCGGC	1 cut(s)	266
NlaIII	CATG	6 cut(s)	569, 818, 986, 1464, 1639, 1907
NlaIV	GGNNCC	3 cut(s)	94, 879, 1582
NmuCI	GTSAC	3 cut(s)	377, 1563, 1820
NsiI	ATGCAT	1 cut(s)	140
NspI	RCATGY	3 cut(s)	569, 818, 986
PaeI	GCATGC	3 cut(s)	569, 818, 986
PagI	TCATGA	1 cut(s)	1635
PceI	AGGCCT	1 cut(s)	1939
PciSI	GCTCTTC	1 cut(s)	177
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 1566
PctI	GAATGC	1 cut(s)	837
PdiI	GCCGGC	1 cut(s)	268
PdmI	GAANNNNTTC	3 cut(s)	186, 209, 894
PfeI	GAWTC	8 cut(s)	149, 176, 210, 545, 737, 825, 1327, 1415
PflMI	CCANNNNNTGG	1 cut(s)	1337
PfoI	TCCNGGA	1 cut(s)	1602
PinAI	ACCGGT	1 cut(s)	796
PkrI	GCNGC	7 cut(s)	265, 283, 286, 297, 306, 513, 610
PleI	GAGTC	2 cut(s)	1464, 1693
PluTI	GGCGCC	1 cut(s)	1584
PpsI	GAGTC	2 cut(s)	1464, 1693
Psp6I	CCWGG	2 cut(s)	1102, 1602
PspGI	CCWGG	2 cut(s)	1102, 1602
PspN4I	GGNNCC	3 cut(s)	94, 879, 1582
PspPI	GGNCC	1 cut(s)	73
PsuI	RGATCY	4 cut(s)	92, 598, 697, 1546
RsaI	GTAC	5 cut(s)	22, 614, 651, 1317, 1491
RsaNI	GTAC	5 cut(s)	21, 613, 650, 1316, 1490
RseI	CAYNNNNRTG	1 cut(s)	1570
SacII	CCGCGG	1 cut(s)	673
SalI	GTCGAC	1 cut(s)	339
SapI	GCTCTTC	1 cut(s)	177
SaqAI	TTAA	7 cut(s)	507, 531, 629, 936, 1026, 1193, 1410
SatI	GCNGC	7 cut(s)	264, 282, 285, 296, 305, 512, 609
Sau96I	GGNCC	1 cut(s)	73
SchI	GAGTC	2 cut(s)	1464, 1693
ScrFI	CCNGG	5 cut(s)	72, 1007, 1008, 1104, 1604
SduI	GDGCHC	2 cut(s)	796, 951
SfaNI	GCATC	5 cut(s)	125, 147, 1123, 1270, 1393
SfoI	GGCGCC	1 cut(s)	1582
Sfr303I	CCGCGG	1 cut(s)	673
SgrAI	CRCCGGYG	1 cut(s)	266
SgrBI	CCGCGG	1 cut(s)	673
SmaI	CCCGGG	1 cut(s)	1008
SmiMI	CAYNNNNRTG	1 cut(s)	1570
SmlI	CTYRAG	2 cut(s)	1192, 1701
SmoI	CTYRAG	2 cut(s)	1192, 1701
SphI	GCATGC	3 cut(s)	569, 818, 986
Sse9I	AATT	8 cut(s)	241, 276, 329, 348, 590, 1027, 1230, 1836
SseBI	AGGCCT	1 cut(s)	1939
SspDI	GGCGCC	1 cut(s)	1580
SspI	AATATT	1 cut(s)	893
SspMI	CTAG	2 cut(s)	941, 1299
StuI	AGGCCT	1 cut(s)	1939
StyD4I	CCNGG	5 cut(s)	70, 1005, 1006, 1102, 1602
StyI	CCWWGG	1 cut(s)	963
TaaI	ACNGT	5 cut(s)	118, 130, 201, 1045, 1126
TaiI	ACGT	2 cut(s)	12, 1323
TasI	AATT	8 cut(s)	241, 276, 329, 348, 590, 1027, 1230, 1836
TatI	WGTACW	2 cut(s)	20, 1315
TauI	GCSGC	2 cut(s)	266, 287
TfiI	GAWTC	8 cut(s)	149, 176, 210, 545, 737, 825, 1327, 1415
Tru1I	TTAA	7 cut(s)	507, 531, 629, 936, 1026, 1193, 1410
Tru9I	TTAA	7 cut(s)	507, 531, 629, 936, 1026, 1193, 1410
TscAI	CASTG	5 cut(s)	981, 1129, 1342, 1360, 1825
TseFI	GTSAC	3 cut(s)	377, 1563, 1820
TseI	GCWGC	5 cut(s)	281, 295, 304, 511, 608
Tsp45I	GTSAC	3 cut(s)	377, 1563, 1820
TspDTI	ATGAA	6 cut(s)	1149, 1175, 1395, 1449, 1546, 1652
TspGWI	ACGGA	2 cut(s)	492, 1477
TspMI	CCCGGG	1 cut(s)	1006
TspRI	CASTG	5 cut(s)	981, 1129, 1342, 1360, 1825
Van91I	CCANNNNNTGG	1 cut(s)	1337
Vha464I	CTTAAG	1 cut(s)	1192
VneI	GTGCAC	1 cut(s)	792
XapI	RAATTY	2 cut(s)	241, 590
XceI	RCATGY	3 cut(s)	569, 818, 986
XcmI	CCANNNNNNNNNTGG	2 cut(s)	86, 922
XmaI	CCCGGG	1 cut(s)	1006
XmiI	GTMKAC	1 cut(s)	340
XmnI	GAANNNNTTC	3 cut(s)	186, 209, 894
XspI	CTAG	2 cut(s)	941, 1299
Zsp2I	ATGCAT	1 cut(s)	140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.