Rorug06G0511000

UFM1-specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008045
Physical Location & Seq
Reverse (-)
62567548 .. 62574048
6501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0511000.1

Sequence Viewer

Length: 5262 bp
ATGACATACTTTACGGATAATGATTCTCTAGTGTTCACACCGCTAAACAATTCTCAAAATCATTATTCACTCAATCTCCAGGTGATCGACTACTGTGCGAACGCAAGCAGCCGAACAGCAATCGAACATCCCAGATTTCGCCACGTGGCATTATCCAAAGGATTCGCGAGGCCGGATTGGAATCCCAAATCATGCTCTTGCTTCGTGATCTTGACGACTGTTGGAGGAAGCGATGATTCGGCGTGTTACGATCTCAAAAATGTGTGGGGGCATGTAGGATGGAATGTCAAGTTTGGGGCGTCTCGGTTTGAGGAATTGTTGATGGAAAATAGAATAGAGGTTTCACATGGCACTGAAATTCCCAGGAAATCGAGATCTTTGGATCTTAAGAGTTTGTACAAGTCCAGGAGTAGAAAAGAGGCTGAAAATCAGAGCCTGAAGAGAAAAGGGAGTGAGGACGACGACGGTGGGGACAAGAAGAAGAGGAGTAGGAAAGAGGTGTCTCTCAGTAGTCTCAAGAATGTTAATACTAGTAGTAGCAAGAAGAGTATAGATAAAGTTTATCATAGAGGGTTGGATTCTGGTTCACATGATCCGGACGCATTCAAATCGGGGTCAAGTCAAAAATTGGATAGCAGTAGTGGGTTAAATGGCGTTTCAAAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCGAGGCGTAAGAGGGGATTCGTGGGGCGAAAAAAATGTGAGGGTGCTCAGCTTTTGAAGCTGTCTGATGAGTCTGCTGGTAGAGCAACTAGTGTTGATCAGAATCATCAGATAGCTAAGTTAAGTGGTGATGATTTGGGTACTCAAGCTGAGGCCTGGAAGGCCATAAGAAACAAAGGTTTGGATGAATGCAAAGAAAATATAAATAGCGAGTTGAATGCAACTTCACATGCAAAGAAAGAAAATGTTCTTGAGAGGCAGTCAGTTGCAAGCAATGGTAATTCATCTTTGAAAAAGTCCCGGAGGAATCGTCGGAAAAGGAAGGACTTGTCACCTGATAGTAAAACTGATGCAAAGGAAGCTGAGCCTTTAGTCAATAGTTCTACTAAAGCATGCCAGACTTCACAAGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCGAGGATGCTTTCATCGAGGTTTGATCCAAGCTGTACTGGGTTTTCATTAAACACCAAGGCTTCTGCGATGCAATCTTCAAACGGGTTGTCTAATCAGTCATCTCCTGGTCAGGATTTTGATAGTCACATGTCGAAGTCATTTTCTGGATCAGAATCTCAATCTGTTGATAATGCTGGTAGAGTGCTGAGGCCCAGGGCCAGGAAGCAGCACAAAGAAAAGAAAGGTACGAGGAAGAGGCGCCACTTCTATGAAATCTTCTTTGGGGACTTGGATGTTTGTTGGGTATTGAACCGTAGGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTATTATGGCCTTGTGAATGCTTATGACAAGGAGAAAAAACTTCACCATATTAGATACGATGACCGTGAAGAAGAATGGGTTGATCTTCAACATGAGAGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAGCAGAAAAAATCATTCATGCAAATTACAAGTTCTGAAGAGAGAGAAGGAAACTTGAAACCCAGAAAAGAGAAGAAAAAGAGAGACTCGATGTCGGAGGACGATAGCTGCATAGGCAGCTGTATGGATTCTGAACCTATTATTTCATGGTTGTCGCGATCTACCCGTCGGATCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACAACTGGTCCATCTCCAAAGTCCGTGCCAACTTTGTCTGACAGTACTGGCACTTTACATGGGTGTTTGAGTGATGTTTCCTCTAGAAGGGATACAAATAAATTATCTTGTAATTCCGGCAGATCATCTGATCCTGTGAGGGAAGAGAAGCCTGCTCCAGAAGGCAACTCCGAAGATAGCAAAATGCCTATTGTTTATTATCGGAAGCGATTGCGCAAGACTGGCTCTGTACTGTCTCAAATATACAAGGATGAGCATGCTTCCATGCATGGACATCATTGTTGTACTTCTGTTACTCCTGTTGAGGAGGTTTGGGACTTGGAAGAGCCAGATGATTTTGTTGTTATATTGGATCGCAGTTGGCCATTGTGGTATAGTGATGATGCAGGGTTGCTAAAGTTAACTCTTCCTTGGATAGAATCAGGAAAAGTCAAATTTAAATGCCTTCCACTGCATTCACTAATGAATGATTCTCTTGGAGTAGAGTTGTTACGGTTCTTTCATGCTGCAATGCTGCTTCGGCATGGTGTAGTGGTGACCACATGGCCAAAGGTTCATTTGGAGATGCTCTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAAGGTTGCTTGAAGCAGGCGGTGGCCTTTGTTTCTCTTATCCTGACATTATTTCATCACCCTAATGAACAGGGGAAGCTTACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTTAAATTCTCCTGTGTTCAGCATCTTGGAAAGCAACTTGTGTCTGCTTTCTACAACTTCTGTCAAGTTAAAAATTCAACATGGATGTACCTAGACAATAAACTCAGGAGGCATTGTTTACTCGCCAAAAAACTGCCTCTGTCTGAATGCACCTTTGATAATATCCAGGCACTTCAAAATGGAATAAATCAGTCACCTTCTATGTCTCTTTGCGGGCAACCCTCTGTCAAGGGTACACGGAAGAGGTCCAGGCAGGGCATTAACTTCATGGGCAGTTCTCGGGAAGTTAGTTTTGTAAATATCAGCCATTCTGCTACTCATTCTGATGAGCTTCACAGAAAGCTTCCCCCACTTGCTCTATCTTTTACAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGTGTTGCTAATATCTGCTTTCAAGACCATGATTCAGAAATTACTCCCGAGAACAATTTGAAGGCTCCAGAAAATGTGGCTACCTCTGGTGGGTCCTGTACTAAAGTGGTAACAGAAACATCTCTTTCTATTTGCAGTCAGCGCGGTCGGTTAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCCTCTTCTAGGACTTTCGCTGGCAGAGATAAATCCAAAACTAGTTCCCGTTCTATTGTGAATGGGCTTACTGTTGAAATTCCATCTTTTGACCATTTTGAGAAGCCTGTGGAGAGGGAGTTACAAAGTGCTCAACAGCCTACAGATTTTCAACAGCCTACAGTTTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGTACAGGGCAGCGAAATAGAAATAATATGTCATCATTTGGAAACCTCGCACATCGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGTAATGGACCGAAGAAGCCCCGAACTCAAGTCTCATACACATTGCCTTGTGGAGGTTTTGATGTCAGCTCTAAGCAAAGAAATGTTCACAAAGGGCTTCCTAATAAGCGAATTAGAAGGGCCAGCGAGAAGAGGTCATCAGACATTTCCAGAGGTTCCGAAAGGAACTTGGAGCTCTTATCCTGTGAGGCAAATGTGTTAATTTCTGCAAGTGACAGAGGGTGGAGAGAATGTGGGGCACGTGTAGTATTAGAACTCTTTGATAATAGTGAGTGGAAGCTTGCTGTAAAACTTTCAGGGACTACGAAGTATTCATACAAAGCACATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGATCTTGGAGTTTCCAGATAGGAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCTGCGTCAGTTAAAAACATACCTATTCCTGGTGTTCGCTTAGTAGAAGATATTGATGATAATGGAACAGAAATAGCATTTCTTCGCAGTTCTACCAAGTACTTTCAGCAGATGAAAACCGATGTTGAGATGGCTTTAGATCCATCTCGTCTCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCAAAATTCTTCAGAAGTTGAAAAGAGTAGCTCAATGGAGATTGGCGAGGAGATGTTTGAGAAGACAATGGACATGTTTGAGAAGGCTGCATATGTTCGGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTATTGGGCCCATCGATTTGATTAAAACCATTTATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCGTCATGGGAGACGTATCAAAAACAAGTGAGGGAGTGGGAGCAAGCTATGACCAAGATGAATAACACACTCGCAAATGGTGGCCGTGAGAAAGCTGCAGCAGTTGAGAAGCCACCGATGTATGCTTTTTGTCTGAAACCACGGGGCCTGGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATTTTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATCCTATTGGAAGGAGATCAAATGGGTTTGCCTATGGTGATGAAAAAATTGCATATTCAGGCCATAACTATGAATCTTTAGATGACTCTCCATTGTCTCAGACGTCGCCCAGGGTTTTTTCACCAAGGGATGCAGCAAACATCTTTATGAGCAATGATGGTTTTGAGAGGAATCATCTTCACAGAATTGACAGAAGCAAGTCAAAGAAATATCGGACAATAGCATCCTCTGTTGACCCCCAGATGGTGTCTCCATATAATCATAGAGTGGTACGAAACAGAAATGGAGTTCATAGAGGGAATATTGGTTTCCCGGAGTGGTCAAGCCAGTCATATTATCAACCAGATGTTGCTCAGAGGCTTGTAAATGCGCATGGCTTTGATCATGATGAGTTTAGGCTTCGTGAGGCATCCGGTGCTGCTCAGCATGCACATAACTTAGCCAAGCGTAAGAGAGAAAATGCTCGCAGATTGTTTTACAGAGCAGATCTAGCAATGCACAAGGCTGTGGTTGCTCTCATGACTGCGGAAGCAATCAAAGCTTCTTCAGAGGACTACGACTATGACTACGACTCTGAGTCCGACTCCTTCTCCATCTCCGACTCTGACACCGATTCAGACAGTGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0019783 GO:0043170 GO:0044238 GO:0070011 GO:0071567 GO:0071704 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C78

Protein Analysis

1753

Amino Acids

198.33

Weight (kDa)

9.09

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1315 - 1410 1.9e-08 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 4736
Acc16I TGCGCA 2 cut(s) 2030, 5001
AccB1I GGYRCC 1 cut(s) 1374
AccII CGCG 3 cut(s) 167, 1765, 3102
AccIII TCCGGA 1 cut(s) 595
AciI CCGC 7 cut(s) 41, 2444, 2751, 3102, 3829, 4604, 5156
AcoI YGGCCR 3 cut(s) 2177, 2360, 4468
AcsI RAATTY 7 cut(s) 357, 2249, 2543, 2611, 3234, 4150, 4577
AcuI CTGAAG 6 cut(s) 458, 1611, 1665, 4140, 4287, 5160
AcvI CACGTG 2 cut(s) 145, 3716
AcyI GRCGYC 4 cut(s) 299, 1375, 4336, 4733
AfiI CCNNNNNNNGG 9 cut(s) 1335, 1902, 3048, 3165, 3527, 3910, 3977, 4550, 4873
AflII CTTAAG 2 cut(s) 386, 2939
AflIII ACRYGT 3 cut(s) 1263, 3715, 4218
AhdI GACNNNNNGTC 2 cut(s) 1699, 5206
AhlI ACTAGT 3 cut(s) 530, 782, 3197
AjuI GAANNNNNNNTTGG 2 cut(s) 1380, 1412
Alw21I GWGCWC 3 cut(s) 742, 3289, 3651
AlwNI CAGNNNCTG 1 cut(s) 436
Ama87I CYCGRG 3 cut(s) 694, 2817, 3005
Aor13HI TCCGGA 1 cut(s) 595
ApaI GGGCCC 2 cut(s) 3904, 4302
ApoI RAATTY 7 cut(s) 357, 2249, 2543, 2611, 3234, 4150, 4577
ArsI GACNNNNNNTTYG 4 cut(s) 1208, 1240, 4550, 4582
AseI ATTAAT 1 cut(s) 2928
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 4 cut(s) 1377, 2031, 3102, 5002
AsuC2I CCSGG 2 cut(s) 994, 4943
AsuHPI GGTGA 9 cut(s) 94, 833, 1017, 1502, 2362, 2474, 2724, 4679, 4743
AvaI CYCGRG 3 cut(s) 694, 2817, 3005
AvaII GGWCC 5 cut(s) 1456, 1823, 2784, 3051, 3482
BaeGI GKGCMC 3 cut(s) 3715, 3904, 4302
BalI TGGCCA 2 cut(s) 2179, 2362
BanI GGYRCC 1 cut(s) 1374
BanII GRGCYC 3 cut(s) 3651, 3904, 4302
BarI GAAGNNNNNNTAC 2 cut(s) 3773, 3805
BbrPI CACGTG 2 cut(s) 145, 3716
BbsI GAAGAC 3 cut(s) 1110, 1435, 4214
Bbv12I GWGCWC 3 cut(s) 742, 3289, 3651
BbvCI CCTCAGC 2 cut(s) 843, 1322
BceAI ACGGC 2 cut(s) 4361, 4455
BcgI CGANNNNNNTGC 6 cut(s) 77, 111, 591, 625, 893, 927
BciVI GTATCC 1 cut(s) 1900
BclI TGATCA 3 cut(s) 790, 4252, 5011
BcnI CCSGG 2 cut(s) 994, 4943
BcuI ACTAGT 3 cut(s) 530, 782, 3197
BfaI CTAG 8 cut(s) 29, 531, 783, 1899, 2630, 3165, 3198, 5120
BfmI CTRYAG 3 cut(s) 3297, 3315, 4482
BfoI RGCGCY 1 cut(s) 1378
BfrI CTTAAG 2 cut(s) 386, 2939
BfuI GTATCC 1 cut(s) 1900
BglI GCCNNNNNGGC 1 cut(s) 854
BglII AGATCT 2 cut(s) 374, 5116
BlpI GCTNAGC 3 cut(s) 741, 1056, 5052
BmcAI AGTACT 2 cut(s) 1861, 4049
Bme18I GGWCC 5 cut(s) 1456, 1823, 2784, 3051, 3482
BmeRI GACNNNNNGTC 2 cut(s) 1699, 5206
BmeT110I CYCGRG 3 cut(s) 694, 2817, 3005
BmrI ACTGGG 2 cut(s) 1182, 3348
BmuI ACTGGG 2 cut(s) 1182, 3348
BpiI GAAGAC 3 cut(s) 1110, 1435, 4214
BplI GAGNNNNNCTC 2 cut(s) 5198, 5230
BpmI CTGGAG 5 cut(s) 62, 1956, 3009, 3174, 4364
Bpu10I CCTNAGC 2 cut(s) 843, 1322
Bpu1102I GCTNAGC 3 cut(s) 741, 1056, 5052
BpuEI CTTGAG 4 cut(s) 500, 822, 965, 3486
BpuMI CCSGG 2 cut(s) 994, 4943
Bsa29I ATCGAT 1 cut(s) 4305
BsaAI YACGTR 2 cut(s) 145, 3716
BsaBI GATNNNNATC 3 cut(s) 180, 795, 1288
BsaHI GRCGYC 4 cut(s) 299, 1375, 4336, 4733
BsaWI WCCGGW 2 cut(s) 595, 5042
Bsc4I CCNNNNNNNGG 9 cut(s) 1335, 1902, 3048, 3165, 3527, 3910, 3977, 4550, 4873
Bse1I ACTGG 9 cut(s) 1177, 1825, 1867, 2041, 2515, 3354, 3895, 4294, 4957
Bse3DI GCAATG 6 cut(s) 973, 2331, 3515, 4253, 4789, 5130
Bse8I GATNNNNATC 3 cut(s) 180, 795, 1288
BseAI TCCGGA 1 cut(s) 595
BseCI ATCGAT 1 cut(s) 4305
BseJI GATNNNNATC 3 cut(s) 180, 795, 1288
BseLI CCNNNNNNNGG 9 cut(s) 1335, 1902, 3048, 3165, 3527, 3910, 3977, 4550, 4873
BseMI GCAATG 6 cut(s) 973, 2331, 3515, 4253, 4789, 5130
BseNI ACTGG 9 cut(s) 1177, 1825, 1867, 2041, 2515, 3354, 3895, 4294, 4957
BseRI GAGGAG 5 cut(s) 499, 1124, 1127, 2135, 4208
BseSI GKGCMC 3 cut(s) 3715, 3904, 4302
BseYI CCCAGC 1 cut(s) 3359
BsgI GTGCAG 2 cut(s) 2895, 4575
Bsh1236I CGCG 3 cut(s) 167, 1765, 3102
Bsh1285I CGRYCG 1 cut(s) 3106
BshNI GGYRCC 1 cut(s) 1374
BshVI ATCGAT 1 cut(s) 4305
BsiEI CGRYCG 1 cut(s) 3106
BsiHKAI GWGCWC 3 cut(s) 742, 3289, 3651
BsiHKCI CYCGRG 3 cut(s) 694, 2817, 3005
BsiSI CCGG 6 cut(s) 173, 596, 994, 1932, 4943, 5043
BslFI GGGAC 6 cut(s) 485, 976, 1415, 2144, 3342, 3787
BslI CCNNNNNNNGG 9 cut(s) 1335, 1902, 3048, 3165, 3527, 3910, 3977, 4550, 4873
BsmBI CGTCTC 3 cut(s) 306, 4103, 4391
BsmFI GGGAC 6 cut(s) 485, 976, 1415, 2144, 3342, 3787
BsmI GAATGC 9 cut(s) 602, 684, 887, 916, 1135, 1489, 2269, 2690, 4365
BsoBI CYCGRG 3 cut(s) 694, 2817, 3005
Bsp120I GGGCCC 2 cut(s) 3900, 4298
Bsp1286I GDGCHC 6 cut(s) 742, 3289, 3651, 3715, 3904, 4302
Bsp13I TCCGGA 1 cut(s) 595
Bsp1407I TGTACA 1 cut(s) 396
Bsp1720I GCTNAGC 3 cut(s) 741, 1056, 5052
Bsp68I TCGCGA 2 cut(s) 167, 1765
BspACI CCGC 7 cut(s) 41, 2444, 2751, 3102, 3829, 4604, 5156
BspDI ATCGAT 1 cut(s) 4305
BspEI TCCGGA 1 cut(s) 595
BspFNI CGCG 3 cut(s) 167, 1765, 3102
BspHI TCATGA 3 cut(s) 4284, 5014, 5148
BspMAI CTGCAG 1 cut(s) 4486
BspQI GCTCTTC 1 cut(s) 2133
BspT107I GGYRCC 1 cut(s) 1374
BspTI CTTAAG 2 cut(s) 386, 2939
BsrDI GCAATG 6 cut(s) 973, 2331, 3515, 4253, 4789, 5130
BsrGI TGTACA 1 cut(s) 396
BsrI ACTGG 9 cut(s) 1177, 1825, 1867, 2041, 2515, 3354, 3895, 4294, 4957
BssNI GRCGYC 4 cut(s) 299, 1375, 4336, 4733
BssT1I CCWWGG 3 cut(s) 1191, 2225, 4754
BstACI GRCGYC 4 cut(s) 299, 1375, 4336, 4733
BstAFI CTTAAG 2 cut(s) 386, 2939
BstAPI GCANNNNNTGC 1 cut(s) 5045
BstAUI TGTACA 1 cut(s) 396
BstBAI YACGTR 2 cut(s) 145, 3716
BstDSI CCRYGG 1 cut(s) 4526
BstEII GGTNACC 1 cut(s) 2350
BstENI CCTNNNNNAGG 4 cut(s) 1900, 3163, 3525, 3908
BstFNI CGCG 3 cut(s) 167, 1765, 3102
BstH2I RGCGCY 1 cut(s) 1378
BstHHI GCGC 4 cut(s) 1377, 2031, 3102, 5002
BstMCI CGRYCG 1 cut(s) 3106
BstNSI RCATGY 7 cut(s) 275, 926, 1089, 1267, 2075, 4222, 5060
BstPI GGTNACC 1 cut(s) 2350
BstSFI CTRYAG 3 cut(s) 3297, 3315, 4482
BstSLI GKGCMC 3 cut(s) 3715, 3904, 4302
BstUI CGCG 3 cut(s) 167, 1765, 3102
BstV2I GAAGAC 3 cut(s) 1110, 1435, 4214
BstX2I RGATCY 5 cut(s) 374, 382, 3870, 4087, 5116
BstXI CCANNNNNNTGG 2 cut(s) 4392, 4637
BstYI RGATCY 5 cut(s) 374, 382, 3870, 4087, 5116
Bsu15I ATCGAT 1 cut(s) 4305
BsuI GTATCC 1 cut(s) 1900
BsuTUI ATCGAT 1 cut(s) 4305
BtgI CCRYGG 1 cut(s) 4526
BtgZI GCGATG 3 cut(s) 246, 1217, 4144
BtsI GCAGTG 2 cut(s) 2264, 3366
BtsIMutI CAGTG 6 cut(s) 351, 2264, 2965, 3366, 3902, 5257
BtuMI TCGCGA 2 cut(s) 167, 1765
CaiI CAGNNNCTG 1 cut(s) 436
CciI TCATGA 3 cut(s) 4284, 5014, 5148
CfoI GCGC 4 cut(s) 1377, 2031, 3102, 5002
ClaI ATCGAT 1 cut(s) 4305
CseI GACGC 4 cut(s) 288, 608, 3942, 4325
DinI GGCGCC 1 cut(s) 1376
DraI TTTAAA 2 cut(s) 2254, 2542
DriI GACNNNNNGTC 2 cut(s) 1699, 5206
EaeI YGGCCR 3 cut(s) 2177, 2360, 4468
Eam1105I GACNNNNNGTC 2 cut(s) 1699, 5206
Ecl136II GAGCTC 1 cut(s) 3649
Eco130I CCWWGG 3 cut(s) 1191, 2225, 4754
Eco147I AGGCCT 1 cut(s) 848
Eco24I GRGCYC 3 cut(s) 3651, 3904, 4302
Eco47I GGWCC 5 cut(s) 1456, 1823, 2784, 3051, 3482
Eco53kI GAGCTC 1 cut(s) 3649
Eco57I CTGAAG 6 cut(s) 458, 1611, 1665, 4140, 4287, 5160
Eco72I CACGTG 2 cut(s) 145, 3716
Eco88I CYCGRG 3 cut(s) 694, 2817, 3005
Eco91I GGTNACC 1 cut(s) 2350
EcoICRI GAGCTC 1 cut(s) 3649
EcoNI CCTNNNNNAGG 4 cut(s) 1900, 3163, 3525, 3908
EcoO109I RGGNCCY 3 cut(s) 3051, 3901, 4531
EcoO65I GGTNACC 1 cut(s) 2350
EcoT14I CCWWGG 3 cut(s) 1191, 2225, 4754
EcoT22I ATGCAT 2 cut(s) 2085, 3921
EcoT38I GRGCYC 3 cut(s) 3651, 3904, 4302
EgeI GGCGCC 1 cut(s) 1376
EheI GGCGCC 1 cut(s) 1376
ErhI CCWWGG 3 cut(s) 1191, 2225, 4754
Esp3I CGTCTC 3 cut(s) 306, 4103, 4391
FaqI GGGAC 6 cut(s) 485, 976, 1415, 2144, 3342, 3787
FauI CCCGC 1 cut(s) 2744
FauNDI CATATG 1 cut(s) 4237
FbaI TGATCA 3 cut(s) 790, 4252, 5011
FriOI GRGCYC 3 cut(s) 3651, 3904, 4302
FspAI RTGCGCAY 1 cut(s) 5001
FspBI CTAG 8 cut(s) 29, 531, 783, 1899, 2630, 3165, 3198, 5120
FspI TGCGCA 2 cut(s) 2030, 5001
GlaI GCGC 4 cut(s) 1376, 2030, 3101, 5001
GsaI CCCAGC 1 cut(s) 3363
GsuI CTGGAG 5 cut(s) 62, 1956, 3009, 3174, 4364
HaeII RGCGCY 1 cut(s) 1378
HapII CCGG 6 cut(s) 173, 596, 994, 1932, 4943, 5043
HgaI GACGC 4 cut(s) 288, 608, 3942, 4325
HhaI GCGC 4 cut(s) 1377, 2031, 3102, 5002
Hin1I GRCGYC 4 cut(s) 299, 1375, 4336, 4733
Hin6I GCGC 4 cut(s) 1375, 2029, 3100, 5000
HinP1I GCGC 4 cut(s) 1375, 2029, 3100, 5000
HincII GTYRAC 3 cut(s) 2217, 3822, 4864
HindII GTYRAC 3 cut(s) 2217, 3822, 4864
HindIII AAGCTT 5 cut(s) 662, 2501, 2879, 3752, 5169
HpaI GTTAAC 1 cut(s) 2217
HpaII CCGG 6 cut(s) 173, 596, 994, 1932, 4943, 5043
HphI GGTGA 9 cut(s) 94, 833, 1017, 1502, 2362, 2474, 2724, 4679, 4743
Hpy166II GTNNAC 9 cut(s) 36, 587, 2217, 2657, 2774, 3562, 3822, 4260, 4864
Hpy8I GTNNAC 9 cut(s) 36, 587, 2217, 2657, 2774, 3562, 3822, 4260, 4864
Hpy99I CGWCG 5 cut(s) 464, 467, 1008, 1779, 4738
HpyCH4IV ACGT 4 cut(s) 144, 3715, 4400, 4733
HpySE526I ACGT 4 cut(s) 144, 3715, 4400, 4733
Hsp92I GRCGYC 4 cut(s) 299, 1375, 4336, 4733
HspAI GCGC 4 cut(s) 1375, 2029, 3100, 5000
KasI GGCGCC 1 cut(s) 1374
Kpn2I TCCGGA 1 cut(s) 595
Ksp22I TGATCA 3 cut(s) 790, 4252, 5011
KspAI GTTAAC 1 cut(s) 2217
LguI GCTCTTC 1 cut(s) 2133
LmnI GCTCC 7 cut(s) 1975, 3028, 3155, 3376, 3646, 3891, 4426
MaeI CTAG 8 cut(s) 29, 531, 783, 1899, 2630, 3165, 3198, 5120
MaeII ACGT 4 cut(s) 144, 3715, 4400, 4733
MflI RGATCY 5 cut(s) 374, 382, 3870, 4087, 5116
MhlI GDGCHC 6 cut(s) 742, 3289, 3651, 3715, 3904, 4302
MlsI TGGCCA 2 cut(s) 2179, 2362
MluNI TGGCCA 2 cut(s) 2179, 2362
Mly113I GGCGCC 1 cut(s) 1375
MlyI GAGTC 7 cut(s) 773, 1688, 4709, 5195, 5207, 5216, 5225
MmeI TCCRAC 9 cut(s) 202, 555, 986, 1683, 1757, 2383, 4396, 5235, 5253
Mox20I TGGCCA 2 cut(s) 2179, 2362
Mph1103I ATGCAT 2 cut(s) 2085, 3921
MroI TCCGGA 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 939
MscI TGGCCA 2 cut(s) 2179, 2362
MslI CAYNNNNRTG 7 cut(s) 1383, 2487, 2862, 4390, 4599, 4698, 4775
Msp20I TGGCCA 2 cut(s) 2179, 2362
MspA1I CMGCKG 2 cut(s) 1728, 2909
MspCI CTTAAG 2 cut(s) 386, 2939
MspI CCGG 6 cut(s) 173, 596, 994, 1932, 4943, 5043
Mva1269I GAATGC 9 cut(s) 602, 684, 887, 916, 1135, 1489, 2269, 2690, 4365
MvnI CGCG 3 cut(s) 167, 1765, 3102
NarI GGCGCC 1 cut(s) 1375
NciI CCSGG 2 cut(s) 994, 4943
NdeI CATATG 1 cut(s) 4237
NmuCI GTSAC 6 cut(s) 1023, 1259, 2350, 2730, 3686, 4566
NruI TCGCGA 2 cut(s) 167, 1765
NsbI TGCGCA 2 cut(s) 2030, 5001
NsiI ATGCAT 2 cut(s) 2085, 3921
NspI RCATGY 7 cut(s) 275, 926, 1089, 1267, 2075, 4222, 5060
PaeI GCATGC 3 cut(s) 1089, 2075, 5060
PagI TCATGA 3 cut(s) 4284, 5014, 5148
PasI CCCWGGG 2 cut(s) 1329, 4740
PceI AGGCCT 1 cut(s) 848
PciI ACATGT 2 cut(s) 1263, 4218
PciSI GCTCTTC 1 cut(s) 2133
PctI GAATGC 9 cut(s) 602, 684, 887, 916, 1135, 1489, 2269, 2690, 4365
PdmI GAANNNNTTC 1 cut(s) 939
PfoI TCCNGGA 3 cut(s) 404, 992, 4941
PleI GAGTC 7 cut(s) 772, 1688, 4709, 5195, 5207, 5215, 5225
PluTI GGCGCC 1 cut(s) 1378
PmaCI CACGTG 2 cut(s) 145, 3716
PmlI CACGTG 2 cut(s) 145, 3716
PpsI GAGTC 7 cut(s) 772, 1688, 4709, 5195, 5207, 5215, 5225
Ppu21I YACGTR 2 cut(s) 145, 3716
PpuMI RGGWCCY 1 cut(s) 3051
PscI ACATGT 2 cut(s) 1263, 4218
PshBI ATTAAT 1 cut(s) 2928
Psp124BI GAGCTC 1 cut(s) 3651
Psp5II RGGWCCY 1 cut(s) 3051
PspCI CACGTG 2 cut(s) 145, 3716
PspEI GGTNACC 1 cut(s) 2350
PspFI CCCAGC 1 cut(s) 3359
PspOMI GGGCCC 2 cut(s) 3900, 4298
PspPPI RGGWCCY 1 cut(s) 3051
PstI CTGCAG 1 cut(s) 4486
PstNI CAGNNNCTG 1 cut(s) 436
PsuI RGATCY 5 cut(s) 374, 382, 3870, 4087, 5116
PvuII CAGCTG 2 cut(s) 1728, 2909
RruI TCGCGA 2 cut(s) 167, 1765
RseI CAYNNNNRTG 7 cut(s) 1383, 2487, 2862, 4390, 4599, 4698, 4775
SacI GAGCTC 1 cut(s) 3651
SapI GCTCTTC 1 cut(s) 2133
ScaI AGTACT 2 cut(s) 1861, 4049
SchI GAGTC 7 cut(s) 773, 1688, 4709, 5195, 5207, 5216, 5225
SduI GDGCHC 6 cut(s) 742, 3289, 3651, 3715, 3904, 4302
SfcI CTRYAG 3 cut(s) 3297, 3315, 4482
SfiI GGCCNNNNNGGCC 1 cut(s) 854
SfoI GGCGCC 1 cut(s) 1376
SinI GGWCC 5 cut(s) 1456, 1823, 2784, 3051, 3482
SmiI ATTTAAAT 1 cut(s) 2254
SmiMI CAYNNNNRTG 7 cut(s) 1383, 2487, 2862, 4390, 4599, 4698, 4775
SmlI CTYRAG 6 cut(s) 386, 515, 837, 944, 2939, 3501
SmoI CTYRAG 6 cut(s) 386, 515, 837, 944, 2939, 3501
SpeI ACTAGT 3 cut(s) 530, 782, 3197
SphI GCATGC 3 cut(s) 1089, 2075, 5060
SseBI AGGCCT 1 cut(s) 848
SsiI CCGC 7 cut(s) 41, 2444, 2751, 3102, 3829, 4604, 5156
SspDI GGCGCC 1 cut(s) 1374
SspI AATATT 1 cut(s) 4933
SspMI CTAG 8 cut(s) 29, 531, 783, 1899, 2630, 3165, 3198, 5120
SstI GAGCTC 1 cut(s) 3651
StuI AGGCCT 1 cut(s) 848
StyI CCWWGG 3 cut(s) 1191, 2225, 4754
SwaI ATTTAAAT 1 cut(s) 2254
TaiI ACGT 4 cut(s) 147, 3718, 4403, 4736
TaqI TCGA 7 cut(s) 87, 123, 371, 1151, 1268, 1697, 4305
TaqII GACCGA 2 cut(s) 3499, 3936
TatI WGTACW 8 cut(s) 396, 1169, 1859, 2044, 2099, 3056, 3380, 4047
TscAI CASTG 6 cut(s) 358, 2271, 2965, 3373, 3902, 5257
TseFI GTSAC 6 cut(s) 1023, 1259, 2350, 2730, 3686, 4566
Tsp45I GTSAC 6 cut(s) 1023, 1259, 2350, 2730, 3686, 4566
TspGWI ACGGA 3 cut(s) 29, 1828, 2791
TspRI CASTG 6 cut(s) 358, 2271, 2965, 3373, 3902, 5257
Vha464I CTTAAG 2 cut(s) 386, 2939
VpaK11BI GGWCC 5 cut(s) 1456, 1823, 2784, 3051, 3482
VspI ATTAAT 1 cut(s) 2928
XagI CCTNNNNNAGG 4 cut(s) 1900, 3163, 3525, 3908
XapI RAATTY 7 cut(s) 357, 2249, 2543, 2611, 3234, 4150, 4577
XbaI TCTAGA 1 cut(s) 1898
XceI RCATGY 7 cut(s) 275, 926, 1089, 1267, 2075, 4222, 5060
XcmI CCANNNNNNNNNTGG 1 cut(s) 3470
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 8 cut(s) 29, 531, 783, 1899, 2630, 3165, 3198, 5120
ZraI GACGTC 1 cut(s) 4734
ZrmI AGTACT 2 cut(s) 1861, 4049
Zsp2I ATGCAT 2 cut(s) 2085, 3921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.