Prupe.5G160400_v2.0.a1

UFM1-specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0008045
Physical Location & Seq
Reverse (-)
14143724 .. 14148476
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G160400.1

Sequence Viewer

Length: 1944 bp
ATGGAGAACGTGTACGAGAATACAAGCGTTCGAGTTCTCTGCTCTAAGCTTGTGCTCTACAAGAACGAACCCGGCCTCCAATGGCTGATCGGATCCCCATTCTTCCCGCCACTTACCGTCGTCTCCACCCTCAGATGCATCCACACCGATGCCTCCTCTCCAGACTACCACAGAGAATCAGAAGAGCTTCGAACGTTACTGTTCAAAGGATTTGAGGTGATCGGAGCATTGATCTTTGCAAATTCCGATGCTGAAAAGAACGCCGGCGAGGCTATTGCTGCGGCTCGAAGGTTGCGAAAGCTTTTCTGCGGTGAAAGAGAAAAAATGGATAGTCAACAAGTAATTGGAGCTGTTGTGGATTTGAACGGCGGCGACATAAAGTTTTTCGTGTCGAAATCCAAGAGTTCTAAGAGCTTTGAATGTGTTAACGTGTTGTATGAAGATGATCCTGAGAAGTATATTTGGGAGAGGGGTTGTCTGCTCCGATGTGAGCTTCCGCTTAAGTTGCCGATCTATTATCCAGTAAACAAGCCGTCTGATTTGGAAAAGATATTTCGGCGTGCAACTGAAGCAGTTGTTGCCAAGTTCAAAGATCCAAAGATTGCATACATGGTAGAAGCATTAAGAAAAACTTCTGCTGAAGTACCTCAACCTGTCATCCTTCGTGGTGTAGATTTGGATTTTGACACAGATCTCTCAAATGTCAAACTTTTGGATGAAAGAACTCAAAATTCTGATGCAGATTCTTTATCATGTTCGCACTTCTGTCTGGAAAGTAAAAAAGGCTCACCAGTATATTCTGCTGTGCATGCAGATAGAATCCAAGTGAGTGTTCTGCTGAATAGGTCAGTTAACTCTGAAAAGTCTACTGCACCTGTTGCAGAATATTTTCCAGCTTTGGAAGAAGCCAGAGTTTTGGTGGTAGATTATAAGCTAGAAGTGCTCTGCTATGCAGTCAAGGGTCTCCCACTGAGGCATGCAGTTTCAAAGTTAATTATCCCAGGGTTGGTTGATCAGTTTAATTTAGTGGGAAATACAGTCCTGCCGAACCTGTTAGCGCAACATCCTCAGCTACATACCTATCACTTTAGCCCTCCTGGTGTTCTGCATCCAATTACTGTTGTCTATGAACTCAATTATGGGGAAACAGAAATGAAGCAAGTTGAAGTGAGGAAATCTTTACATTTAAGACTGGGTTTACCTTTCGATCGGCCTCTTTTAAGAATTGCCAACGCTGTGGATTTCTTTGCGAGGGATGGCGTAAGGAGTGACCCGACACGGAAGGGTTCTACTTTGCTCAAAGATGTACACCTTGGGATTCCTAGCAGTGGGGTTTCAGGGGGCAGCGTGTCTATGGTGCAAGGCTCGTATGAATACTACCATTATCTACAAGAAGCATTTAATGATTCAGGGTGGGGTTGTGCTTATCGTTCTCTACAAACTATAATTTCATGGTTCAGAATCCAACATTATACATCCGTAGAAGTTCCATCGCATAGGGAAATACAGCAGACACTTGTAGAGATTGGTGATAAAGATCCTTCTTTCATTGGTTCACGCGAATGGATTGGTGCCATTGAATTGAGCTTTGTTCTGGACAAGCTTTTGGGTGTTAGTTGCAAAGTCATAAATGTTAGATCTGGAGCTGAGCTTCCAGAGAAATGTCGAGAGCTGGCCTTGCACTTTGACAATCAAGGAACACCTATTATGATAGGTGGTGGTGTTCTTGCATATACCCTCTTGGGAATTGATTACAATGAAGCAAGTGGAGATTGTGCGTTTCTAATACTCGATCCTCACTATACCGGCAACGACGAGCACAAGAAAATTGTAAACGGCGGATGGTGTGGGTGGAAAAAGGCTGTTGATAGCAAGGGAAAGAGTTTCTTTTTGCATGATAAGTTCTATAATCTTCTGCTTCCACAGAGGCCTGACATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0019783 GO:0043170 GO:0044238 GO:0070011 GO:0071567 GO:0071704 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C78

Protein Analysis

648

Amino Acids

72.44

Weight (kDa)

6.25

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
AccB1I GGYRCC 1 cut(s) 1571
AccI GTMKAC 1 cut(s) 866
AccII CGCG 1 cut(s) 1560
AciI CCGC 6 cut(s) 107, 281, 309, 369, 497, 1839
AclI AACGTT 1 cut(s) 194
AclWI GGATC 6 cut(s) 87, 100, 440, 587, 1532, 1787
AcsI RAATTY 2 cut(s) 241, 730
AcuI CTGAAG 2 cut(s) 588, 660
AfaI GTAC 3 cut(s) 14, 645, 1308
AfiI CCNNNNNNNGG 2 cut(s) 1006, 1328
AflII CTTAAG 1 cut(s) 500
AflIII ACRYGT 2 cut(s) 9, 429
AgsI TTSAA 7 cut(s) 205, 364, 419, 589, 987, 1166, 1580
AjnI CCWGG 2 cut(s) 1000, 1096
AjuI GAANNNNNNNTTGG 2 cut(s) 816, 848
AloI GAACNNNNNNTCC 2 cut(s) 60, 92
Alw21I GWGCWC 3 cut(s) 57, 945, 1821
Alw26I GTCTC 2 cut(s) 127, 968
AlwI GGATC 6 cut(s) 87, 100, 440, 587, 1532, 1787
AoxI GGCC 4 cut(s) 73, 1211, 1674, 1928
ApeKI GCWGC 2 cut(s) 278, 1344
ApoI RAATTY 2 cut(s) 241, 730
Asp700I GAANNNNTTC 3 cut(s) 186, 631, 888
AspLEI GCGC 1 cut(s) 1060
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 4 cut(s) 229, 323, 780, 1541
AsuII TTCGAA 1 cut(s) 190
BamHI GGATCC 1 cut(s) 92
BanI GGYRCC 1 cut(s) 1571
Bbv12I GWGCWC 3 cut(s) 57, 945, 1821
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 2 cut(s) 265, 1356
BccI CCATC 3 cut(s) 1250, 1498, 1836
BceAI ACGGC 3 cut(s) 382, 517, 1852
BcgI CGANNNNNNTGC 4 cut(s) 21, 55, 257, 291
BciT130I CCWGG 2 cut(s) 1002, 1098
BclI TGATCA 1 cut(s) 1012
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 127, 968
BfaI CTAG 2 cut(s) 935, 1323
BfrI CTTAAG 1 cut(s) 500
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 2 cut(s) 691, 1637
BisI GCNGC 4 cut(s) 279, 282, 370, 1345
BlpI GCTNAGC 1 cut(s) 1647
BlsI GCNGC 4 cut(s) 280, 283, 371, 1346
Bme1390I CCNGG 3 cut(s) 72, 1002, 1098
BmiI GGNNCC 2 cut(s) 94, 1573
BmrFI CCNGG 3 cut(s) 72, 1002, 1098
BmrI ACTGGG 1 cut(s) 1202
BmsI GCATC 6 cut(s) 125, 139, 147, 238, 727, 1117
BmuI ACTGGG 1 cut(s) 1202
BpmI CTGGAG 2 cut(s) 144, 1662
Bpu10I CCTNAGC 1 cut(s) 1068
Bpu1102I GCTNAGC 1 cut(s) 1647
Bpu14I TTCGAA 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 1536
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 3 cut(s) 1000, 1001, 1312
BsaXI ACNNNNNCTCC 5 cut(s) 26, 60, 90, 339, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 1006, 1328
Bse118I RCCGGY 2 cut(s) 263, 1805
Bse1I ACTGG 3 cut(s) 521, 791, 1197
Bse8I GATNNNNATC 1 cut(s) 1536
BseBI CCWGG 2 cut(s) 1002, 1098
BseDI CCNNGG 3 cut(s) 1000, 1001, 1312
BseGI GGATG 8 cut(s) 138, 657, 721, 1063, 1108, 1261, 1475, 1847
BseJI GATNNNNATC 1 cut(s) 1536
BseLI CCNNNNNNNGG 2 cut(s) 1006, 1328
BseMII CTCAG 5 cut(s) 145, 441, 962, 1082, 1638
BseNI ACTGG 3 cut(s) 521, 791, 1197
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 265, 1356
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 1560
Bsh1285I CGRYCG 1 cut(s) 1210
BshFI GGCC 4 cut(s) 75, 1213, 1676, 1930
BshNI GGYRCC 1 cut(s) 1571
BsiEI CGRYCG 1 cut(s) 1210
BsiHKAI GWGCWC 3 cut(s) 57, 945, 1821
BsiSI CCGG 3 cut(s) 72, 264, 1806
BslI CCNNNNNNNGG 2 cut(s) 1006, 1328
BsmAI GTCTC 2 cut(s) 127, 968
BsmBI CGTCTC 1 cut(s) 127
BsnI GGCC 4 cut(s) 75, 1213, 1676, 1930
Bso31I GGTCTC 1 cut(s) 968
Bsp119I TTCGAA 1 cut(s) 190
Bsp1286I GDGCHC 3 cut(s) 57, 945, 1821
Bsp1407I TGTACA 1 cut(s) 1306
Bsp1720I GCTNAGC 1 cut(s) 1647
BspACI CCGC 6 cut(s) 107, 281, 309, 369, 497, 1839
BspANI GGCC 4 cut(s) 75, 1213, 1676, 1930
BspCNI CTCAG 5 cut(s) 144, 442, 963, 1081, 1639
BspFNI CGCG 1 cut(s) 1560
BspLI GGNNCC 2 cut(s) 94, 1573
BspPI GGATC 6 cut(s) 87, 100, 440, 587, 1532, 1787
BspQI GCTCTTC 1 cut(s) 177
BspT104I TTCGAA 1 cut(s) 190
BspT107I GGYRCC 1 cut(s) 1571
BspTI CTTAAG 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 968
BsrFI RCCGGY 2 cut(s) 263, 1805
BsrGI TGTACA 1 cut(s) 1306
BsrI ACTGG 3 cut(s) 521, 791, 1197
BssAI RCCGGY 2 cut(s) 263, 1805
BssECI CCNNGG 3 cut(s) 1000, 1001, 1312
BssT1I CCWWGG 1 cut(s) 1312
Bst2UI CCWGG 2 cut(s) 1002, 1098
Bst4CI ACNGT 4 cut(s) 118, 201, 1039, 1120
Bst6I CTCTTC 1 cut(s) 177
BstAFI CTTAAG 1 cut(s) 500
BstAPI GCANNNNNTGC 2 cut(s) 578, 878
BstAUI TGTACA 1 cut(s) 1306
BstBI TTCGAA 1 cut(s) 190
BstC8I GCNNGC 5 cut(s) 265, 561, 810, 978, 1674
BstDEI CTNAG 7 cut(s) 45, 131, 408, 450, 971, 1068, 1647
BstF5I GGATG 8 cut(s) 138, 657, 721, 1063, 1108, 1261, 1475, 1847
BstFNI CGCG 1 cut(s) 1560
BstHHI GCGC 1 cut(s) 1060
BstMAI GTCTC 2 cut(s) 127, 968
BstMCI CGRYCG 1 cut(s) 1210
BstMWI GCNNNNNNNGC 9 cut(s) 269, 278, 505, 569, 578, 809, 878, 940, 1678
BstNI CCWGG 2 cut(s) 1002, 1098
BstNSI RCATGY 2 cut(s) 812, 980
BstSCI CCNGG 3 cut(s) 70, 1000, 1096
BstUI CGCG 1 cut(s) 1560
BstV1I GCAGC 2 cut(s) 265, 1356
BstX2I RGATCY 5 cut(s) 92, 592, 691, 1537, 1637
BstXI CCANNNNNNTGG 2 cut(s) 916, 1237
BstYI RGATCY 5 cut(s) 92, 592, 691, 1537, 1637
BsuRI GGCC 4 cut(s) 75, 1213, 1676, 1930
BtgZI GCGATG 1 cut(s) 1476
BtsCI GGATG 8 cut(s) 138, 657, 721, 1063, 1108, 1261, 1475, 1847
BtsI GCAGTG 1 cut(s) 1333
BtsIMutI CAGTG 2 cut(s) 968, 1333
Cac8I GCNNGC 5 cut(s) 265, 561, 810, 978, 1674
CfoI GCGC 1 cut(s) 1060
Cfr10I RCCGGY 2 cut(s) 263, 1805
Csp6I GTAC 3 cut(s) 13, 644, 1307
CviAII CATG 7 cut(s) 610, 753, 809, 977, 1452, 1895, 1936
CviQI GTAC 3 cut(s) 13, 644, 1307
DdeI CTNAG 7 cut(s) 45, 131, 408, 450, 971, 1068, 1647
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
EciI GGCGGA 1 cut(s) 1854
Eco130I CCWWGG 1 cut(s) 1312
Eco147I AGGCCT 1 cut(s) 1930
Eco31I GGTCTC 1 cut(s) 968
Eco57I CTGAAG 2 cut(s) 588, 660
EcoRII CCWGG 2 cut(s) 1000, 1096
EcoT14I CCWWGG 1 cut(s) 1312
EcoT22I ATGCAT 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 1312
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 7 cut(s) 613, 756, 812, 980, 1455, 1898, 1939
FalI AAGNNNNNCTT 4 cut(s) 616, 648, 1871, 1903
FatI CATG 7 cut(s) 609, 752, 808, 976, 1451, 1894, 1935
FauI CCCGC 1 cut(s) 114
FbaI TGATCA 1 cut(s) 1012
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 4 cut(s) 279, 282, 370, 1345
FokI GGATG 8 cut(s) 125, 644, 728, 1050, 1095, 1268, 1462, 1854
Fsp4HI GCNGC 4 cut(s) 279, 282, 370, 1345
FspBI CTAG 2 cut(s) 935, 1323
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 4 cut(s) 279, 282, 370, 1345
GsuI CTGGAG 2 cut(s) 144, 1662
HaeIII GGCC 4 cut(s) 75, 1213, 1676, 1930
HapII CCGG 3 cut(s) 72, 264, 1806
HhaI GCGC 1 cut(s) 1060
Hin1II CATG 7 cut(s) 613, 756, 812, 980, 1455, 1898, 1939
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HincII GTYRAC 3 cut(s) 335, 427, 853
HindII GTYRAC 3 cut(s) 335, 427, 853
HindIII AAGCTT 3 cut(s) 47, 299, 1601
HinfI GANTC 6 cut(s) 176, 743, 819, 1318, 1406, 1461
HpaI GTTAAC 2 cut(s) 427, 853
HpaII CCGG 3 cut(s) 72, 264, 1806
HphI GGTGA 4 cut(s) 229, 323, 780, 1541
Hpy188III TCNNGA 7 cut(s) 161, 449, 770, 1595, 1641, 1655, 1667
Hpy99I CGWCG 2 cut(s) 122, 1817
HpyAV CCTTC 4 cut(s) 282, 671, 1276, 1551
HpyCH4III ACNGT 4 cut(s) 118, 201, 1039, 1120
HpyCH4IV ACGT 3 cut(s) 9, 194, 429
HpyF10VI GCNNNNNNNGC 9 cut(s) 269, 278, 505, 569, 578, 809, 878, 940, 1678
HpyF3I CTNAG 7 cut(s) 45, 131, 408, 450, 971, 1068, 1647
HpySE526I ACGT 3 cut(s) 9, 194, 429
Hsp92II CATG 7 cut(s) 613, 756, 812, 980, 1455, 1898, 1939
HspAI GCGC 1 cut(s) 1058
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 1012
KspAI GTTAAC 2 cut(s) 427, 853
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 4 cut(s) 224, 347, 486, 1643
Lsp1109I GCAGC 2 cut(s) 265, 1356
LweI GCATC 6 cut(s) 125, 139, 147, 238, 727, 1117
MaeI CTAG 2 cut(s) 935, 1323
MaeII ACGT 3 cut(s) 9, 194, 429
MaeIII GTNAC 2 cut(s) 195, 1268
MboII GAAGA 5 cut(s) 94, 194, 452, 914, 1904
MflI RGATCY 5 cut(s) 92, 592, 691, 1537, 1637
MhlI GDGCHC 3 cut(s) 57, 945, 1821
MmeI TCCRAC 1 cut(s) 1489
Mph1103I ATGCAT 1 cut(s) 140
MreI CGCCGGCG 1 cut(s) 263
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 3 cut(s) 186, 631, 888
MseI TTAA 9 cut(s) 426, 501, 623, 852, 992, 1020, 1187, 1220, 1401
MslI CAYNNNNRTG 2 cut(s) 147, 1561
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 3 cut(s) 72, 264, 1806
MspR9I CCNGG 3 cut(s) 72, 1002, 1098
MvaI CCWGG 2 cut(s) 1002, 1098
MvnI CGCG 1 cut(s) 1560
MwoI GCNNNNNNNGC 9 cut(s) 269, 278, 505, 569, 578, 809, 878, 940, 1678
NaeI GCCGGC 1 cut(s) 265
NciI CCSGG 1 cut(s) 72
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 7 cut(s) 613, 756, 812, 980, 1455, 1898, 1939
NlaIV GGNNCC 2 cut(s) 94, 1573
NmuCI GTSAC 1 cut(s) 1268
NsiI ATGCAT 1 cut(s) 140
NspI RCATGY 2 cut(s) 812, 980
NspV TTCGAA 1 cut(s) 190
PaeI GCATGC 2 cut(s) 812, 980
PasI CCCWGGG 1 cut(s) 1001
PceI AGGCCT 1 cut(s) 1930
PciSI GCTCTTC 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 3 cut(s) 186, 631, 888
PfeI GAWTC 6 cut(s) 176, 743, 819, 1318, 1406, 1461
PkrI GCNGC 4 cut(s) 280, 283, 371, 1346
Ple19I CGATCG 1 cut(s) 1210
PsiI TTATAA 1 cut(s) 930
Psp1406I AACGTT 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 1000, 1096
PspGI CCWGG 2 cut(s) 1000, 1096
PspN4I GGNNCC 2 cut(s) 94, 1573
PsuI RGATCY 5 cut(s) 92, 592, 691, 1537, 1637
PvuI CGATCG 1 cut(s) 1210
RsaI GTAC 3 cut(s) 14, 645, 1308
RsaNI GTAC 3 cut(s) 13, 644, 1307
RseI CAYNNNNRTG 2 cut(s) 147, 1561
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 9 cut(s) 426, 501, 623, 852, 992, 1020, 1187, 1220, 1401
SatI GCNGC 4 cut(s) 279, 282, 370, 1345
ScrFI CCNGG 3 cut(s) 72, 1002, 1098
SduI GDGCHC 3 cut(s) 57, 945, 1821
SfaNI GCATC 6 cut(s) 125, 139, 147, 238, 727, 1117
SfuI TTCGAA 1 cut(s) 190
SgrAI CRCCGGYG 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 147, 1561
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SphI GCATGC 2 cut(s) 812, 980
SseBI AGGCCT 1 cut(s) 1930
SsiI CCGC 6 cut(s) 107, 281, 309, 369, 497, 1839
SspI AATATT 1 cut(s) 887
SspMI CTAG 2 cut(s) 935, 1323
StuI AGGCCT 1 cut(s) 1930
StyD4I CCNGG 3 cut(s) 70, 1000, 1096
StyI CCWWGG 1 cut(s) 1312
TaaI ACNGT 4 cut(s) 118, 201, 1039, 1120
TaiI ACGT 3 cut(s) 12, 197, 432
TaqI TCGA 7 cut(s) 31, 190, 286, 392, 1206, 1666, 1791
TatI WGTACW 1 cut(s) 1306
TauI GCSGC 2 cut(s) 284, 372
TfiI GAWTC 6 cut(s) 176, 743, 819, 1318, 1406, 1461
Tru1I TTAA 9 cut(s) 426, 501, 623, 852, 992, 1020, 1187, 1220, 1401
Tru9I TTAA 9 cut(s) 426, 501, 623, 852, 992, 1020, 1187, 1220, 1401
TscAI CASTG 2 cut(s) 975, 1333
TseFI GTSAC 1 cut(s) 1268
TseI GCWGC 2 cut(s) 278, 1344
Tsp45I GTSAC 1 cut(s) 1268
TspDTI ATGAA 8 cut(s) 453, 732, 1143, 1169, 1386, 1440, 1537, 1773
TspGWI ACGGA 2 cut(s) 1294, 1468
TspRI CASTG 2 cut(s) 975, 1333
Vha464I CTTAAG 1 cut(s) 500
XapI RAATTY 2 cut(s) 241, 730
XceI RCATGY 2 cut(s) 812, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XmiI GTMKAC 1 cut(s) 866
XmnI GAANNNNTTC 3 cut(s) 186, 631, 888
XspI CTAG 2 cut(s) 935, 1323
Zsp2I ATGCAT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.