pycom14g13880

UFM1-specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008045
Physical Location & Seq
Forward (+)
16711933 .. 16716124
4192 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g13880.12

Sequence Viewer

Length: 1923 bp
ATGGCTGATCGATCCCCATTTTTCCCGCCACTCACAGTCGTCTCCACCGTCAGATGCATCCACACCGATTCCTCCTCTCCAGACTACCGCAGAGAATCAGAAGAGCTTCGGACGTTACTGTTGAAAGGATTTGAGGTGATCGGGGCACTAATCGTTGCGAATTCCGGTGATGGAAAGAGCGCCGCTGGCGAGGCAATTGCGGCGGCTCGAAGGTTGCGAAGGCTTCTCTCCGGAGAAGGAGGAAAACTGGACAGTCAACAAGTAATTGGAGCTGTTGCGGAATTTAGTGGCGGCGACATTCAGTTTTTCGTGTCTAAATCCGCGAGCTTGACCAGCTTTGAAGCTGTGAATGTATTGTATGAAGATCATCCTGAAAAGTATGTTTGGGAGAGGGGTTGTCTGCTCCGGTGTGAGCTTCCATTTAAGCTCCCAATCTACTATCCGGTAAACATACCAAATGATTCAGAAAAGATGTTTCGACGTGCAACTGAAGCAGTTATTGCAAAATTCAAAGATCCAAAGGCTGCGTACATGGTAGAAGCATTAAGCAAAACATCTGCTGAAGTACCTCAACCTGTCATCCTCCGCGGTGTAGATTTGGATTTTGACACAGATCTCTCAAATGTCAAAATTTTTGGAGAAAGTGCTCAAGAGTCCGATGCTGATCTTTTGTCATGTTCCCACTTCTGTTTGGATAGTAAAAAAGGTGCACCGGTATATTCTGCTGAGCATGCAGATAGAATCCAAGTAAGTGTTCTGCTGAATAGTTCAGATAAGTCTGAAAAATCGACAGCCCCCGTTGCTCAATATTTTCCAGCTTTGGAAGAAGCCAGAATTTTGGTGGTAGATTTTAAGCTAGAAGTGCTTTGCTATGCGGTCAAGGGTCTCCCGCTGAGGCATGCAGTTTCGAAGTTAATGATCCCGGGGTTGATTGATCAGTTTAATTTAGTGAAAAATACAGTCCTACCCAACCTCTTAGCCCAACATCCTCAGCTACATCCGTATCACTTCAGTCCTCCCGGTGTTCTGCATCCAATCACTGTTATCTATGAACTCAACTACGGGGAGACAGAAATGAAGCAAGTTGAAGTTAGAAAATCTTTACACCTAAGACTGGGTTTACCTTTTGATCGTCCTCTTCTAAGAATTGCCAACGCTCTGGATTTCTCTACATTGAGGGATGGTGTAAGGAGTGACGCAACACGGAAAGGTTCTACTTTGCTTAAAGATGTACATATCGGGATTCCAAGCAGCGGTGTTTCTGGGGGCAGTGTGTCTTTGTTGCAAGGCTCTTATGAATACCATCATTATCTACAAGATGCCTTCAATGATTCGGGTTGGGGTTGTGCTTATCGGTCTCTACAGACGATTATTTCATGGTTCCGACTCCAACACTATACATCCGTAGAAGTTCCATCGCATAGGGAAATACAGCAGTCACTTGTAGAGATTGGTGATAAAGATCCCTCTTTCATTGGGTCGCGGGAATGGATTGGTGCCATCGAGTTGAGCTTTGTTCTGGACAAGCTTTTGGGTGTTAGTTGCAAAGTCATGAATGTCAGATCTGGAGCTGAGCTTCCCGAAAAATGTCGAGAGCTGGCCTTGCACTTTGAGACTCAAGGAACGCCTATTATGATCGGTGGTGGAGTTCTTGCATATACCCTCTTGGGAGTTGATTACAATGAAGCAAGCGGAGATTGCGCGTTTCTAATTCTCGATCCCCACTACACAGGCAGTGACGAACACAAGAAAATTGTGAGTGGCGGATGGTGCGGGTGGAAAAAGGCTGTCGATAGCAAGGGAAAGAGTTTCTTTCTGCACGATAAGTTCTATAATCTTTTGCTTCCACAGAGGCCTAACATGGTTTTAAGATCGATCTATCACTATCATCATCAAATCTATACACCCTGTTATGAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0019783 GO:0043170 GO:0044238 GO:0070011 GO:0071567 GO:0071704 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C78

Protein Analysis

641

Amino Acids

71.16

Weight (kDa)

6.2

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1496
AccII CGCG 4 cut(s) 323, 588, 1483, 1703
AccIII TCCGGA 1 cut(s) 230
AclWI GGATC 5 cut(s) 6, 509, 913, 1457, 1712
AcsI RAATTY 5 cut(s) 160, 281, 506, 630, 834
AcuI CTGAAG 3 cut(s) 510, 582, 994
AfaI GTAC 3 cut(s) 530, 567, 1233
AfiI CCNNNNNNNGG 2 cut(s) 1114, 1253
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 5 cut(s) 124, 341, 511, 1088, 1327
AjiI CACGTC 1 cut(s) 482
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
Alw21I GWGCWC 2 cut(s) 649, 712
Alw26I GTCTC 5 cut(s) 46, 890, 1061, 1362, 1607
Alw44I GTGCAC 1 cut(s) 708
AlwI GGATC 5 cut(s) 6, 509, 913, 1457, 1712
Ama87I CYCGRG 1 cut(s) 922
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 1599, 1853
ApaLI GTGCAC 1 cut(s) 708
ApeKI GCWGC 2 cut(s) 524, 1251
ApoI RAATTY 5 cut(s) 160, 281, 506, 630, 834
AsiGI ACCGGT 1 cut(s) 712
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 2 cut(s) 182, 1703
AsuC2I CCSGG 3 cut(s) 923, 924, 1020
AsuHPI GGTGA 3 cut(s) 148, 179, 1466
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 1 cut(s) 922
BaeGI GKGCMC 2 cut(s) 148, 712
BaeI ACNNNNGTAYC 2 cut(s) 986, 1019
BanI GGYRCC 1 cut(s) 1496
Bbv12I GWGCWC 2 cut(s) 649, 712
BbvCI CCTCAGC 2 cut(s) 893, 990
BbvI GCAGC 2 cut(s) 511, 1263
BccI CCATC 6 cut(s) 164, 1175, 1311, 1423, 1508, 1761
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BclI TGATCA 1 cut(s) 934
BcnI CCSGG 3 cut(s) 923, 924, 1020
BcoDI GTCTC 5 cut(s) 46, 890, 1061, 1362, 1607
BfaI CTAG 1 cut(s) 857
BfmI CTRYAG 1 cut(s) 1361
BfoI RGCGCY 1 cut(s) 183
BglII AGATCT 2 cut(s) 613, 1562
BisI GCNGC 6 cut(s) 183, 201, 204, 292, 525, 1252
BlpI GCTNAGC 2 cut(s) 726, 1572
BlsI GCNGC 6 cut(s) 184, 202, 205, 293, 526, 1253
Bme1390I CCNGG 3 cut(s) 923, 924, 1020
BmeT110I CYCGRG 1 cut(s) 922
BmgBI CACGTC 1 cut(s) 482
BmiI GGNNCC 2 cut(s) 1382, 1498
BmrFI CCNGG 3 cut(s) 923, 924, 1020
BmrI ACTGGG 1 cut(s) 1124
BmsI GCATC 5 cut(s) 44, 66, 649, 1039, 1309
BmuI ACTGGG 1 cut(s) 1124
BpmI CTGGAG 2 cut(s) 63, 1587
Bpu10I CCTNAGC 2 cut(s) 893, 990
Bpu1102I GCTNAGC 2 cut(s) 726, 1572
Bpu14I TTCGAA 1 cut(s) 908
BpuEI CTTGAG 2 cut(s) 633, 1602
BpuMI CCSGG 3 cut(s) 923, 924, 1020
Bsa29I ATCGAT 2 cut(s) 10, 1874
BsaBI GATNNNNATC 2 cut(s) 663, 1461
BsaI GGTCTC 2 cut(s) 890, 1362
BsaJI CCNNGG 3 cut(s) 586, 922, 923
BsaWI WCCGGW 5 cut(s) 164, 230, 405, 442, 712
Bsc4I CCNNNNNNNGG 2 cut(s) 1114, 1253
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 2 cut(s) 252, 1119
Bse8I GATNNNNATC 2 cut(s) 663, 1461
BseAI TCCGGA 1 cut(s) 230
BseCI ATCGAT 2 cut(s) 10, 1874
BseDI CCNNGG 3 cut(s) 586, 922, 923
BseGI GGATG 9 cut(s) 57, 367, 579, 985, 997, 1030, 1186, 1400, 1772
BseJI GATNNNNATC 2 cut(s) 663, 1461
BseLI CCNNNNNNNGG 2 cut(s) 1114, 1253
BseMII CTCAG 4 cut(s) 717, 884, 1004, 1563
BseNI ACTGG 2 cut(s) 252, 1119
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 2 cut(s) 148, 712
BseXI GCAGC 2 cut(s) 511, 1263
BsgI GTGCAG 1 cut(s) 1802
Bsh1236I CGCG 4 cut(s) 323, 588, 1483, 1703
BshFI GGCC 2 cut(s) 1601, 1855
BshNI GGYRCC 1 cut(s) 1496
BshTI ACCGGT 1 cut(s) 712
BshVI ATCGAT 2 cut(s) 10, 1874
BsiHKAI GWGCWC 2 cut(s) 649, 712
BsiHKCI CYCGRG 1 cut(s) 922
BsiSI CCGG 7 cut(s) 165, 231, 406, 443, 713, 923, 1020
BslI CCNNNNNNNGG 2 cut(s) 1114, 1253
BsmAI GTCTC 5 cut(s) 46, 890, 1061, 1362, 1607
BsmBI CGTCTC 1 cut(s) 46
BsnI GGCC 2 cut(s) 1601, 1855
Bso31I GGTCTC 2 cut(s) 890, 1362
BsoBI CYCGRG 1 cut(s) 922
Bsp119I TTCGAA 1 cut(s) 908
Bsp1286I GDGCHC 3 cut(s) 148, 649, 712
Bsp13I TCCGGA 1 cut(s) 230
Bsp1407I TGTACA 1 cut(s) 1231
Bsp1720I GCTNAGC 2 cut(s) 726, 1572
BspANI GGCC 2 cut(s) 1601, 1855
BspCNI CTCAG 4 cut(s) 718, 885, 1003, 1564
BspDI ATCGAT 2 cut(s) 10, 1874
BspEI TCCGGA 1 cut(s) 230
BspFNI CGCG 4 cut(s) 323, 588, 1483, 1703
BspHI TCATGA 1 cut(s) 1551
BspLI GGNNCC 2 cut(s) 1382, 1498
BspPI GGATC 5 cut(s) 6, 509, 913, 1457, 1712
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 908
BspT107I GGYRCC 1 cut(s) 1496
BspTNI GGTCTC 2 cut(s) 890, 1362
BsrFI RCCGGY 1 cut(s) 712
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 2 cut(s) 252, 1119
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 3 cut(s) 586, 922, 923
Bst4CI ACNGT 6 cut(s) 37, 49, 120, 254, 961, 1042
Bst6I CTCTTC 2 cut(s) 96, 1143
BstAPI GCANNNNNTGC 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 1231
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 6 cut(s) 187, 325, 732, 900, 1599, 1690
BstDEI CTNAG 7 cut(s) 726, 893, 976, 990, 1109, 1142, 1572
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 9 cut(s) 57, 367, 579, 985, 997, 1030, 1186, 1400, 1772
BstFNI CGCG 4 cut(s) 323, 588, 1483, 1703
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 182, 1703
BstMAI GTCTC 5 cut(s) 46, 890, 1061, 1362, 1607
BstNSI RCATGY 2 cut(s) 734, 902
BstSCI CCNGG 3 cut(s) 921, 922, 1018
BstSFI CTRYAG 1 cut(s) 1361
BstSLI GKGCMC 2 cut(s) 148, 712
BstUI CGCG 4 cut(s) 323, 588, 1483, 1703
BstV1I GCAGC 2 cut(s) 511, 1263
BstX2I RGATCY 4 cut(s) 514, 613, 1462, 1562
BstXI CCANNNNNNTGG 2 cut(s) 838, 1159
BstYI RGATCY 4 cut(s) 514, 613, 1462, 1562
Bsu15I ATCGAT 2 cut(s) 10, 1874
BsuRI GGCC 2 cut(s) 1601, 1855
BsuTUI ATCGAT 2 cut(s) 10, 1874
BtgI CCRYGG 1 cut(s) 586
BtgZI GCGATG 1 cut(s) 1401
BtrI CACGTC 1 cut(s) 482
BtsCI GGATG 9 cut(s) 57, 367, 579, 985, 997, 1030, 1186, 1400, 1772
BtsI GCAGTG 2 cut(s) 1276, 1741
BtsIMutI CAGTG 3 cut(s) 1038, 1276, 1741
Cac8I GCNNGC 6 cut(s) 187, 325, 732, 900, 1599, 1690
CciI TCATGA 1 cut(s) 1551
CfoI GCGC 2 cut(s) 182, 1703
Cfr10I RCCGGY 1 cut(s) 712
Cfr42I CCGCGG 1 cut(s) 589
Cfr9I CCCGGG 1 cut(s) 922
ClaI ATCGAT 2 cut(s) 10, 1874
CseI GACGC 1 cut(s) 1205
Csp6I GTAC 3 cut(s) 529, 566, 1232
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 7 cut(s) 532, 675, 731, 899, 1377, 1552, 1861
CviQI GTAC 3 cut(s) 529, 566, 1232
DdeI CTNAG 7 cut(s) 726, 893, 976, 990, 1109, 1142, 1572
Eam1104I CTCTTC 2 cut(s) 96, 1143
EarI CTCTTC 2 cut(s) 96, 1143
EciI GGCGGA 1 cut(s) 1779
Eco147I AGGCCT 1 cut(s) 1855
Eco31I GGTCTC 2 cut(s) 890, 1362
Eco57I CTGAAG 3 cut(s) 510, 582, 994
Eco88I CYCGRG 1 cut(s) 922
EcoRI GAATTC 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 59
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 7 cut(s) 535, 678, 734, 902, 1380, 1555, 1864
FalI AAGNNNNNCTT 2 cut(s) 1796, 1828
FatI CATG 7 cut(s) 531, 674, 730, 898, 1376, 1551, 1860
FauI CCCGC 4 cut(s) 33, 897, 1476, 1766
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 6 cut(s) 183, 201, 204, 292, 525, 1252
FokI GGATG 9 cut(s) 44, 354, 566, 972, 984, 1017, 1193, 1387, 1779
Fsp4HI GCNGC 6 cut(s) 183, 201, 204, 292, 525, 1252
FspBI CTAG 1 cut(s) 857
GlaI GCGC 2 cut(s) 181, 1702
GluI GCNGC 6 cut(s) 183, 201, 204, 292, 525, 1252
GsuI CTGGAG 2 cut(s) 63, 1587
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 1601, 1855
HapII CCGG 7 cut(s) 165, 231, 406, 443, 713, 923, 1020
HgaI GACGC 1 cut(s) 1205
HhaI GCGC 2 cut(s) 182, 1703
Hin1II CATG 7 cut(s) 535, 678, 734, 902, 1380, 1555, 1864
Hin6I GCGC 2 cut(s) 180, 1701
HinP1I GCGC 2 cut(s) 180, 1701
HincII GTYRAC 1 cut(s) 257
HindII GTYRAC 1 cut(s) 257
HindIII AAGCTT 1 cut(s) 1526
HinfI GANTC 9 cut(s) 68, 95, 461, 653, 741, 1243, 1331, 1386, 1615
HpaII CCGG 7 cut(s) 165, 231, 406, 443, 713, 923, 1020
HphI GGTGA 3 cut(s) 148, 179, 1466
Hpy166II GTNNAC 4 cut(s) 257, 448, 710, 1121
Hpy188I TCNGA 9 cut(s) 53, 100, 111, 466, 658, 772, 781, 1385, 1562
Hpy8I GTNNAC 4 cut(s) 257, 448, 710, 1121
Hpy99I CGWCG 1 cut(s) 483
HpyAV CCTTC 4 cut(s) 204, 213, 230, 1333
HpyCH4III ACNGT 6 cut(s) 37, 49, 120, 254, 961, 1042
HpyCH4IV ACGT 2 cut(s) 113, 481
HpyF3I CTNAG 7 cut(s) 726, 893, 976, 990, 1109, 1142, 1572
HpySE526I ACGT 2 cut(s) 113, 481
Hsp92II CATG 7 cut(s) 535, 678, 734, 902, 1380, 1555, 1864
HspAI GCGC 2 cut(s) 180, 1701
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 934
KspI CCGCGG 1 cut(s) 589
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 4 cut(s) 269, 408, 432, 1568
Lsp1109I GCAGC 2 cut(s) 511, 1263
LweI GCATC 5 cut(s) 44, 66, 649, 1039, 1309
MaeI CTAG 1 cut(s) 857
MaeII ACGT 2 cut(s) 113, 481
MaeIII GTNAC 4 cut(s) 114, 1193, 1437, 1736
MboII GAAGA 4 cut(s) 113, 374, 836, 1130
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 4 cut(s) 514, 613, 1462, 1562
MhlI GDGCHC 3 cut(s) 148, 649, 712
MlyI GAGTC 3 cut(s) 662, 1380, 1609
MmeI TCCRAC 2 cut(s) 1408, 1414
Mph1103I ATGCAT 1 cut(s) 59
MroI TCCGGA 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 7 cut(s) 423, 545, 852, 914, 942, 1224, 1868
MspA1I CMGCKG 4 cut(s) 185, 588, 892, 1254
MspI CCGG 7 cut(s) 165, 231, 406, 443, 713, 923, 1020
MspR9I CCNGG 3 cut(s) 923, 924, 1020
MunI CAATTG 1 cut(s) 195
MvnI CGCG 4 cut(s) 323, 588, 1483, 1703
NciI CCSGG 3 cut(s) 923, 924, 1020
NlaIII CATG 7 cut(s) 535, 678, 734, 902, 1380, 1555, 1864
NlaIV GGNNCC 2 cut(s) 1382, 1498
NmuCI GTSAC 3 cut(s) 1193, 1437, 1736
NsiI ATGCAT 1 cut(s) 59
NspI RCATGY 2 cut(s) 734, 902
NspV TTCGAA 1 cut(s) 908
PaeI GCATGC 2 cut(s) 734, 902
PagI TCATGA 1 cut(s) 1551
PceI AGGCCT 1 cut(s) 1855
PciSI GCTCTTC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 214
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 6 cut(s) 68, 95, 461, 741, 1243, 1331
PinAI ACCGGT 1 cut(s) 712
PkrI GCNGC 6 cut(s) 184, 202, 205, 293, 526, 1253
PleI GAGTC 3 cut(s) 661, 1380, 1609
PpsI GAGTC 3 cut(s) 661, 1380, 1609
PspN4I GGNNCC 2 cut(s) 1382, 1498
PsuI RGATCY 4 cut(s) 514, 613, 1462, 1562
RsaI GTAC 3 cut(s) 530, 567, 1233
RsaNI GTAC 3 cut(s) 529, 566, 1232
SacII CCGCGG 1 cut(s) 589
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 7 cut(s) 423, 545, 852, 914, 942, 1224, 1868
SatI GCNGC 6 cut(s) 183, 201, 204, 292, 525, 1252
SchI GAGTC 3 cut(s) 662, 1380, 1609
ScrFI CCNGG 3 cut(s) 923, 924, 1020
SduI GDGCHC 3 cut(s) 148, 649, 712
SfaNI GCATC 5 cut(s) 44, 66, 649, 1039, 1309
SfcI CTRYAG 1 cut(s) 1361
Sfr303I CCGCGG 1 cut(s) 589
SfuI TTCGAA 1 cut(s) 908
SgrBI CCGCGG 1 cut(s) 589
SmaI CCCGGG 1 cut(s) 924
SmlI CTYRAG 2 cut(s) 648, 1617
SmoI CTYRAG 2 cut(s) 648, 1617
SphI GCATGC 2 cut(s) 734, 902
SseBI AGGCCT 1 cut(s) 1855
SspI AATATT 1 cut(s) 809
SspMI CTAG 1 cut(s) 857
StuI AGGCCT 1 cut(s) 1855
StyD4I CCNGG 3 cut(s) 921, 922, 1018
TaaI ACNGT 6 cut(s) 37, 49, 120, 254, 961, 1042
TaiI ACGT 2 cut(s) 116, 484
TaqII GACCGA 1 cut(s) 1344
TatI WGTACW 1 cut(s) 1231
TauI GCSGC 4 cut(s) 185, 203, 206, 294
TfiI GAWTC 6 cut(s) 68, 95, 461, 741, 1243, 1331
Tru1I TTAA 7 cut(s) 423, 545, 852, 914, 942, 1224, 1868
Tru9I TTAA 7 cut(s) 423, 545, 852, 914, 942, 1224, 1868
TscAI CASTG 3 cut(s) 1045, 1276, 1741
TseFI GTSAC 3 cut(s) 1193, 1437, 1736
TseI GCWGC 2 cut(s) 524, 1251
Tsp45I GTSAC 3 cut(s) 1193, 1437, 1736
TspDTI ATGAA 8 cut(s) 375, 1065, 1091, 1311, 1365, 1462, 1568, 1698
TspGWI ACGGA 3 cut(s) 990, 1219, 1393
TspMI CCCGGG 1 cut(s) 922
TspRI CASTG 3 cut(s) 1045, 1276, 1741
VneI GTGCAC 1 cut(s) 708
XapI RAATTY 5 cut(s) 160, 281, 506, 630, 834
XceI RCATGY 2 cut(s) 734, 902
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XmaI CCCGGG 1 cut(s) 922
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 1 cut(s) 857
Zsp2I ATGCAT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.