MD06G1223100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013063
Physical Location & Seq
Reverse (-)
35426725 .. 35428021
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1223100.v1.1.491

Sequence Viewer

Length: 369 bp
ATGCTCATATTTACTGCTGAGAAGGAATTGAAGGGCGGTCGCTACTTCCCTGTTACCGCAGTTCAGCGATTTGATGCTGCTGGAAAGAGAATTGAGAACGGAGTGTATTTCGGGCCATTTGGATGCTTAACATTTGAAGGAAGATTTTCCTGGAAGGAGAGAATACTAGCCTTCGTCTTTGAGTCCATCCGGATAAAAATTGGACCTTTGAAAACCCTAGAGATCGGTCTAGGCCAGAAAGACGACAGAGAGCCTAGCACGAAGGATCCATTCTTTATATGGTTTTACATTGATGAAGAACTAGCAGTTGGCCGAGGCAAACGTGGGGGAACTGCTTTCTGGTGCCGTTGTCACCGTGTAATGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

14.07

Weight (kDa)

9.3

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013063)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18060
fragaria_vesca FvH4_5g16950 FvH4_5g16950 FvH4_5g16950
malus_domestica MD06G1223100.v1.1
prunus_persica Prupe.5G228200_v2.0.a1
pyrus_communis pycom14g19360
rosa_chinensis RchiOBHm_Chr7g0179071
rosa_laevigata RLG00000005388
rosa_multiflora Rmu_sc0000888.1_g000015
rosa_roxburghii Rroxscaffold_3G00274420
rosa_rugosa Rorug06G0420600 Rorug06G0420700.1
rosa_samantha Rh7AG020100 Rh7BG019800 Rh7CG021100 Rh7DG020600
rosa_wichuraiana Rw7G001670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 2 cut(s) 36, 57
AclWI GGATC 2 cut(s) 260, 273
AcoI YGGCCR 1 cut(s) 310
AgsI TTSAA 3 cut(s) 31, 137, 211
AjnI CCWGG 1 cut(s) 149
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AlwI GGATC 2 cut(s) 260, 273
Aor13HI TCCGGA 1 cut(s) 189
AoxI GGCC 3 cut(s) 113, 232, 310
ApeKI GCWGC 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 113, 203
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 1 cut(s) 203
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 194
BceAI ACGGC 1 cut(s) 330
BciT130I CCWGG 1 cut(s) 151
BfaI CTAG 5 cut(s) 167, 218, 230, 255, 302
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 151
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 113, 203
BmiI GGNNCC 2 cut(s) 267, 344
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 64, 113
BsaJI CCNNGG 1 cut(s) 313
BsaWI WCCGGW 1 cut(s) 189
BseAI TCCGGA 1 cut(s) 189
BseBI CCWGG 1 cut(s) 151
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 2 cut(s) 128, 186
BseMII CTCAG 1 cut(s) 9
BseXI GCAGC 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 3 cut(s) 115, 234, 312
BshNI GGYRCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 190
BsnI GGCC 3 cut(s) 115, 234, 312
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 2 cut(s) 222, 265
BspACI CCGC 2 cut(s) 36, 57
BspANI GGCC 3 cut(s) 115, 234, 312
BspCNI CTCAG 1 cut(s) 10
BspEI TCCGGA 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 267, 344
BspPI GGATC 2 cut(s) 260, 273
BspT107I GGYRCC 1 cut(s) 342
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 2 cut(s) 222, 265
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 128, 186
BstKTI GATC 2 cut(s) 225, 268
BstMBI GATC 2 cut(s) 222, 265
BstMCI CGRYCG 1 cut(s) 40
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 3 cut(s) 115, 234, 312
BtsCI GGATG 2 cut(s) 128, 186
Cfr13I GGNCC 2 cut(s) 113, 203
CviJI RGCY 5 cut(s) 115, 170, 234, 253, 312
CviKI_1 RGCY 5 cut(s) 115, 170, 234, 253, 312
DdeI CTNAG 1 cut(s) 18
DpnI GATC 2 cut(s) 224, 267
DpnII GATC 2 cut(s) 222, 265
EaeI YGGCCR 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 149
FaiI YATR 3 cut(s) 8, 278, 280
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 2 cut(s) 135, 173
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 5 cut(s) 167, 218, 230, 255, 302
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 3 cut(s) 115, 234, 312
HapII CCGG 1 cut(s) 190
HinfI GANTC 1 cut(s) 182
HpaII CCGG 1 cut(s) 190
HphI GGTGA 1 cut(s) 344
Hpy188III TCNNGA 1 cut(s) 190
HpyAV CCTTC 6 cut(s) 16, 25, 131, 148, 181, 256
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4IV ACGT 1 cut(s) 322
HpyF3I CTNAG 1 cut(s) 18
HpySE526I ACGT 1 cut(s) 322
Kpn2I TCCGGA 1 cut(s) 189
Kzo9I GATC 2 cut(s) 222, 265
LpnPI CCDG 7 cut(s) 63, 66, 136, 163, 203, 248, 325
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 64, 113
MaeI CTAG 5 cut(s) 167, 218, 230, 255, 302
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 2 cut(s) 52, 350
MalI GATC 2 cut(s) 224, 267
MboI GATC 2 cut(s) 222, 265
MboII GAAGA 2 cut(s) 153, 308
MflI RGATCY 1 cut(s) 265
MluCI AATT 3 cut(s) 26, 90, 198
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 1 cut(s) 308
MroI TCCGGA 1 cut(s) 189
MroXI GAANNNNTTC 1 cut(s) 145
MseI TTAA 2 cut(s) 128, 367
MslI CAYNNNNRTG 1 cut(s) 121
MspI CCGG 1 cut(s) 190
MspR9I CCNGG 1 cut(s) 151
MvaI CCWGG 1 cut(s) 151
NdeII GATC 2 cut(s) 222, 265
NlaIV GGNNCC 2 cut(s) 267, 344
NmeAIII GCCGAG 1 cut(s) 338
NmuCI GTSAC 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 149
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 267, 344
PspPI GGNCC 2 cut(s) 113, 203
PsuI RGATCY 1 cut(s) 265
RseI CAYNNNNRTG 1 cut(s) 121
SaqAI TTAA 2 cut(s) 128, 367
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 222, 265
Sau96I GGNCC 2 cut(s) 113, 203
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 151
SetI ASST 2 cut(s) 208, 325
SfaNI GCATC 2 cut(s) 64, 113
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 121
Sse9I AATT 3 cut(s) 26, 90, 198
SsiI CCGC 2 cut(s) 36, 57
SspMI CTAG 5 cut(s) 167, 218, 230, 255, 302
StyD4I CCNGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 356
TaiI ACGT 1 cut(s) 325
TaqII GACCGA 1 cut(s) 215
TasI AATT 3 cut(s) 26, 90, 198
Tru1I TTAA 2 cut(s) 128, 367
Tru9I TTAA 2 cut(s) 128, 367
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 1 cut(s) 309
TspGWI ACGGA 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 203
XcmI CCANNNNNNNNNTGG 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 145
XspI CTAG 5 cut(s) 167, 218, 230, 255, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.