MD06G1226600.v1.1

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014745
Physical Location & Seq
Forward (+)
35720939 .. 35723387
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1226600.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGCATGCCAATCATCAACAATATGGGGAACAAAAACTGAATCTGCTTCCAAAATTTCAAGCTGCTTTAACAACAGCTCCTCCGTGCTCCTCTTCTCCTCCGCCATTCGCAACAAAAGCCGCCGTTTCCTCACTTGTTCATTAGACCCCATCTCCACTTCATCAACAACATCTTCTTCTTCAGCCTCACATAAAGCTACTTGCAAGAACATAACAAACAGAAAGTCTCTTGAAGTACAAAGAACCAAGGATGGGGCTTCCTCGGTTTTGCATGGCGATGAGCGTAGACAATCCGATAAGAAGATTAAGAAGAGCAAGAAGGATGAAACTAAGAATGGCGAGATTATGGGATCGAAATCTGGATCAAGAAAGCGGCCATCGTCGTGGAAGAAGGTTTTCTTTTCATCCAAGAAGTTTAGGAGTATTATGCTGCTCAATTTCATCACAATTGTATATGCAAGTGACATTCCAGTTATTAAAGAAGTTGAAGCTTTCATGGATCCAGCAGCCTTCTCAGCAGTACGATTTGTCATGTCCGCCATCCCATTTCTTCCATTTGTGTTTCGATCGCGAGGTGATGTCCAGACTCGCAATTCAGGCATGGAGTTGGGGTTCTGGATCAGCCTAGGATACCTTCTTGAGGCACTTGGTTTACTTACATCTGATGCTGGTCGTGCATCATTTATTTCTCTGTTCACAGTTATCACGGTACCTTTGCTTGATGGCATGCTTGGAGCAACCATACCTACTCGTACCTGGTTCGGAGTCCTCATGTCTGCTCTCGGGATTGCTATGCTCGAATGCAGCGGATCACCTCCGAATGTTGGAGATCTTTTGAACTTCTTGAGTGCAATATTTTTCGGAATTCACATGCTTCGAACTGAACATATTTCTAGAAGCACAAAGAAAGAAAACTTCTTAGCAATTCTTGGATTTGAGGTTTGTGTTGTTGCTATGTTATCAACAATTTGGGTTTTCATCGGAGGGTGGTTTGATGGTACCAACTATTCCAACCAATCCCCATGGACATGGACAGAGCTATGGGACTGGCTTGTTGTATTTCCATGGATACCAGCAATTTATACTGGTGCATTTTCAACCGGATTATGCTTATGGATTGAGATTGCAGCGATGTCTGATGTTTCAGCAACAGAAACAGCCATAATTTACGGGATGGAACCACTCTGGGGTGCTGGTTTTGCTTGGTTTCTGCTTGGTGAAAGATGGGGCATGCTTGGATGGTTAGGCGCTGCTCTTGTTCTTGGTGGAAGCTTACTGGTGCAGATTTTCGGGTCCTCATCCCCCAAAGAATTTACGGTGGCTAAGGATGGTAATGAGAAAGGTGATCTTGATCTCCAACGAGTTCCAGTCGAGCATAAATTACAAAACGGACTCTCTACTTCACCGGTTGTCGTCAGATCTGAAAAGAATGTCACAGATTTCTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

484

Amino Acids

53.15

Weight (kDa)

9.2

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 143 - 266 9.5e-10 EamA-like transporter family
EamA PF00892 276 - 427 3.1e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17290 FvH4_5g17290 FvH4_5g17290
malus_domestica MD06G1226600.v1.1
prunus_persica Prupe.5G232800_v2.0.a1 Prupe.5G232900_v2.0.a1
pyrus_communis pycom06g20290
rosa_chinensis RchiOBHm_Chr7g0178481
rosa_laevigata RLG00000005443
rosa_multiflora Rmu_sc0010235.1_g000003
rosa_roxburghii Rroxscaffold_3G00274860
rosa_rugosa Rorug06G0417500
rosa_samantha Rh7AG016000 Rh7BG015600 Rh7CG016300 Rh7DG016000
rosa_wichuraiana Rw7G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 709, 998
AccB1I GGYRCC 2 cut(s) 709, 998
AccI GTMKAC 1 cut(s) 286
AccII CGCG 1 cut(s) 571
AciI CCGC 5 cut(s) 102, 121, 373, 537, 807
AclWI GGATC 6 cut(s) 358, 370, 494, 507, 626, 817
AcoI YGGCCR 1 cut(s) 374
AcsI RAATTY 3 cut(s) 54, 864, 1310
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 5 cut(s) 237, 522, 711, 754, 1000
AfiI CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
AgeI ACCGGT 1 cut(s) 1405
AgsI TTSAA 6 cut(s) 60, 233, 488, 838, 1098, 1447
AjnI CCWGG 1 cut(s) 755
AloI GAACNNNNNNTCC 2 cut(s) 596, 628
AluBI AGCT 6 cut(s) 63, 78, 197, 491, 1039, 1272
AluI AGCT 6 cut(s) 63, 78, 197, 491, 1039, 1272
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 6 cut(s) 358, 370, 494, 507, 626, 817
Ama87I CYCGRG 1 cut(s) 782
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 6 cut(s) 63, 430, 506, 804, 1127, 1250
ApoI RAATTY 3 cut(s) 54, 864, 1310
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AsiGI ACCGGT 1 cut(s) 1405
Asp700I GAANNNNTTC 1 cut(s) 395
Asp718I GGTACC 2 cut(s) 709, 998
AspA2I CCTAGG 1 cut(s) 625
AspLEI GCGC 1 cut(s) 1250
AspS9I GGNCC 1 cut(s) 1293
AsuHPI GGTGA 5 cut(s) 587, 804, 1229, 1355, 1395
AsuII TTCGAA 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 782
AvaII GGWCC 1 cut(s) 1293
AvrII CCTAGG 1 cut(s) 625
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 2 cut(s) 709, 998
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 757
BciVI GTATCC 2 cut(s) 623, 1062
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 2 cut(s) 626, 894
BfoI RGCGCY 1 cut(s) 1251
BfuI GTATCC 2 cut(s) 623, 1062
BglII AGATCT 2 cut(s) 829, 1418
BisI GCNGC 8 cut(s) 64, 121, 374, 431, 507, 805, 1128, 1251
BlnI CCTAGG 1 cut(s) 625
BlsI GCNGC 8 cut(s) 65, 122, 375, 432, 508, 806, 1129, 1252
Bme1390I CCNGG 1 cut(s) 757
Bme18I GGWCC 1 cut(s) 1293
BmeT110I CYCGRG 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 1293
BmiI GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
BmrFI CCNGG 1 cut(s) 757
BmsI GCATC 2 cut(s) 655, 686
Bpu10I CCTNAGC 1 cut(s) 1323
Bpu14I TTCGAA 1 cut(s) 877
BpuEI CTTGAG 2 cut(s) 659, 865
BsaBI GATNNNNATC 2 cut(s) 355, 1350
BsaJI CCNNGG 5 cut(s) 246, 261, 625, 1022, 1064
BsaWI WCCGGW 2 cut(s) 1100, 1405
BsaXI ACNNNNNCTCC 8 cut(s) 62, 65, 92, 95, 137, 167, 596, 626
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
Bse118I RCCGGY 1 cut(s) 1405
Bse1I ACTGG 5 cut(s) 470, 1052, 1090, 1281, 1367
Bse8I GATNNNNATC 2 cut(s) 355, 1350
BseBI CCWGG 1 cut(s) 757
BseDI CCNNGG 5 cut(s) 246, 261, 625, 1022, 1064
BseGI GGATG 8 cut(s) 256, 328, 404, 540, 1179, 1244, 1298, 1333
BseJI GATNNNNATC 2 cut(s) 355, 1350
BseLI CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 5 cut(s) 470, 1052, 1090, 1281, 1367
BseRI GAGGAG 3 cut(s) 70, 80, 88
BseXI GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
BsgI GTGCAG 1 cut(s) 1301
Bsh1236I CGCG 1 cut(s) 571
Bsh1285I CGRYCG 1 cut(s) 569
BshFI GGCC 1 cut(s) 376
BshNI GGYRCC 2 cut(s) 709, 998
BshTI ACCGGT 1 cut(s) 1405
BsiEI CGRYCG 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 782
BsiSI CCGG 2 cut(s) 1101, 1406
BslFI GGGAC 1 cut(s) 1058
BslI CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
BsmAI GTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 1058
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 877
Bsp1286I GDGCHC 1 cut(s) 90
Bsp19I CCATGG 2 cut(s) 1022, 1064
Bsp68I TCGCGA 1 cut(s) 571
BspACI CCGC 5 cut(s) 102, 121, 373, 537, 807
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 1 cut(s) 527
BspFNI CGCG 1 cut(s) 571
BspLI GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
BspPI GGATC 6 cut(s) 358, 370, 494, 507, 626, 817
BspQI GCTCTTC 1 cut(s) 305
BspT104I TTCGAA 1 cut(s) 877
BspT107I GGYRCC 2 cut(s) 709, 998
BsrFI RCCGGY 1 cut(s) 1405
BsrI ACTGG 5 cut(s) 470, 1052, 1090, 1281, 1367
BssAI RCCGGY 1 cut(s) 1405
BssECI CCNNGG 5 cut(s) 246, 261, 625, 1022, 1064
BssT1I CCWWGG 4 cut(s) 246, 625, 1022, 1064
Bst2UI CCWGG 1 cut(s) 757
Bst4CI ACNGT 3 cut(s) 700, 709, 1318
Bst6I CTCTTC 2 cut(s) 98, 305
BstBI TTCGAA 1 cut(s) 877
BstC8I GCNNGC 3 cut(s) 7, 728, 1232
BstDEI CTNAG 4 cut(s) 330, 514, 919, 1323
BstDSI CCRYGG 2 cut(s) 1022, 1064
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 8 cut(s) 256, 328, 404, 540, 1179, 1244, 1298, 1333
BstFNI CGCG 1 cut(s) 571
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 1 cut(s) 1250
BstMAI GTCTC 1 cut(s) 231
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 5 cut(s) 117, 515, 597, 674, 1199
BstNI CCWGG 1 cut(s) 757
BstNSI RCATGY 4 cut(s) 9, 730, 874, 1234
BstSCI CCNGG 1 cut(s) 755
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
BstX2I RGATCY 3 cut(s) 499, 829, 1418
BstXI CCANNNNNNTGG 2 cut(s) 384, 1029
BstYI RGATCY 3 cut(s) 499, 829, 1418
BsuI GTATCC 2 cut(s) 623, 1062
BsuRI GGCC 1 cut(s) 376
BtgI CCRYGG 2 cut(s) 1022, 1064
BtgZI GCGATG 2 cut(s) 291, 1145
BtsCI GGATG 8 cut(s) 256, 328, 404, 540, 1179, 1244, 1298, 1333
BtuMI TCGCGA 1 cut(s) 571
Cac8I GCNNGC 3 cut(s) 7, 728, 1232
CfoI GCGC 1 cut(s) 1250
Cfr10I RCCGGY 1 cut(s) 1405
Cfr13I GGNCC 1 cut(s) 1293
CsiI ACCWGGT 1 cut(s) 755
Csp6I GTAC 5 cut(s) 236, 521, 710, 753, 999
CspAI ACCGGT 1 cut(s) 1405
CviQI GTAC 5 cut(s) 236, 521, 710, 753, 999
DdeI CTNAG 4 cut(s) 330, 514, 919, 1323
EaeI YGGCCR 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 98, 305
EarI CTCTTC 2 cut(s) 98, 305
EciI GGCGGA 2 cut(s) 91, 526
Eco130I CCWWGG 4 cut(s) 246, 625, 1022, 1064
Eco47I GGWCC 1 cut(s) 1293
Eco57I CTGAAG 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 782
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 1293
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 1 cut(s) 755
EcoT14I CCWWGG 4 cut(s) 246, 625, 1022, 1064
ErhI CCWWGG 4 cut(s) 246, 625, 1022, 1064
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 1332, 1364
FaqI GGGAC 1 cut(s) 1058
FblI GTMKAC 1 cut(s) 286
Fnu4HI GCNGC 8 cut(s) 64, 121, 374, 431, 507, 805, 1128, 1251
FokI GGATG 8 cut(s) 263, 335, 391, 527, 1186, 1251, 1285, 1340
Fsp4HI GCNGC 8 cut(s) 64, 121, 374, 431, 507, 805, 1128, 1251
FspBI CTAG 2 cut(s) 626, 894
GlaI GCGC 1 cut(s) 1249
GluI GCNGC 8 cut(s) 64, 121, 374, 431, 507, 805, 1128, 1251
HaeII RGCGCY 1 cut(s) 1251
HaeIII GGCC 1 cut(s) 376
HapII CCGG 2 cut(s) 1101, 1406
HhaI GCGC 1 cut(s) 1250
Hin6I GCGC 1 cut(s) 1248
HinP1I GCGC 1 cut(s) 1248
HindIII AAGCTT 2 cut(s) 489, 1270
HinfI GANTC 4 cut(s) 41, 586, 765, 1392
HpaII CCGG 2 cut(s) 1101, 1406
HphI GGTGA 5 cut(s) 587, 804, 1229, 1355, 1395
Hpy166II GTNNAC 3 cut(s) 287, 653, 696
Hpy188I TCNGA 9 cut(s) 295, 664, 764, 819, 863, 983, 1138, 1418, 1423
Hpy8I GTNNAC 3 cut(s) 287, 653, 696
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 4 cut(s) 313, 385, 520, 644
HpyCH4III ACNGT 3 cut(s) 700, 709, 1318
HpyCH4V TGCA 9 cut(s) 204, 271, 458, 677, 804, 851, 1091, 1127, 1282
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 515, 597, 674, 1199
HpyF3I CTNAG 4 cut(s) 330, 514, 919, 1323
HspAI GCGC 1 cut(s) 1248
KpnI GGTACC 2 cut(s) 713, 1002
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 3 cut(s) 83, 93, 734
Lsp1109I GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
LweI GCATC 2 cut(s) 655, 686
MabI ACCWGGT 1 cut(s) 755
MaeI CTAG 2 cut(s) 626, 894
MaeIII GTNAC 2 cut(s) 461, 1432
MboII GAAGA 9 cut(s) 85, 165, 168, 171, 313, 322, 400, 542, 1435
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 3 cut(s) 499, 829, 1418
MhlI GDGCHC 1 cut(s) 90
MlyI GAGTC 3 cut(s) 580, 774, 1386
MmeI TCCRAC 3 cut(s) 805, 1035, 1381
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 3 cut(s) 69, 306, 477
MslI CAYNNNNRTG 3 cut(s) 276, 382, 1027
MspA1I CMGCKG 1 cut(s) 807
MspI CCGG 2 cut(s) 1101, 1406
MspR9I CCNGG 1 cut(s) 757
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 1 cut(s) 757
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 5 cut(s) 117, 515, 597, 674, 1199
NcoI CCATGG 2 cut(s) 1022, 1064
NlaIV GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
NmuCI GTSAC 2 cut(s) 461, 1432
NruI TCGCGA 1 cut(s) 571
NspI RCATGY 4 cut(s) 9, 730, 874, 1234
NspV TTCGAA 1 cut(s) 877
PaeI GCATGC 3 cut(s) 9, 730, 1234
PciSI GCTCTTC 1 cut(s) 305
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 41
PinAI ACCGGT 1 cut(s) 1405
PkrI GCNGC 8 cut(s) 65, 122, 375, 432, 508, 806, 1129, 1252
Ple19I CGATCG 1 cut(s) 569
PleI GAGTC 3 cut(s) 580, 773, 1386
PpsI GAGTC 3 cut(s) 580, 773, 1386
PpuMI RGGWCCY 1 cut(s) 1293
Psp5II RGGWCCY 1 cut(s) 1293
Psp6I CCWGG 1 cut(s) 755
PspGI CCWGG 1 cut(s) 755
PspN4I GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
PspPI GGNCC 1 cut(s) 1293
PspPPI RGGWCCY 1 cut(s) 1293
PsuI RGATCY 3 cut(s) 499, 829, 1418
PvuI CGATCG 1 cut(s) 569
RruI TCGCGA 1 cut(s) 571
RsaI GTAC 5 cut(s) 237, 522, 711, 754, 1000
RsaNI GTAC 5 cut(s) 236, 521, 710, 753, 999
RseI CAYNNNNRTG 3 cut(s) 276, 382, 1027
SapI GCTCTTC 1 cut(s) 305
SaqAI TTAA 3 cut(s) 69, 306, 477
SatI GCNGC 8 cut(s) 64, 121, 374, 431, 507, 805, 1128, 1251
Sau96I GGNCC 1 cut(s) 1293
SchI GAGTC 3 cut(s) 580, 774, 1386
ScrFI CCNGG 1 cut(s) 757
SduI GDGCHC 1 cut(s) 90
SexAI ACCWGGT 1 cut(s) 755
SfaNI GCATC 2 cut(s) 655, 686
SfuI TTCGAA 1 cut(s) 877
SinI GGWCC 1 cut(s) 1293
SmiMI CAYNNNNRTG 3 cut(s) 276, 382, 1027
SmlI CTYRAG 2 cut(s) 638, 844
SmoI CTYRAG 2 cut(s) 638, 844
SphI GCATGC 3 cut(s) 9, 730, 1234
SsiI CCGC 5 cut(s) 102, 121, 373, 537, 807
SspI AATATT 1 cut(s) 855
SspMI CTAG 2 cut(s) 626, 894
StyD4I CCNGG 1 cut(s) 755
StyI CCWWGG 4 cut(s) 246, 625, 1022, 1064
TaaI ACNGT 3 cut(s) 700, 709, 1318
TaqI TCGA 5 cut(s) 353, 565, 798, 877, 1371
TatI WGTACW 1 cut(s) 235
TauI GCSGC 2 cut(s) 123, 376
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 3 cut(s) 69, 306, 477
Tru9I TTAA 3 cut(s) 69, 306, 477
TseFI GTSAC 2 cut(s) 461, 1432
TseI GCWGC 6 cut(s) 63, 430, 506, 804, 1127, 1250
Tsp45I GTSAC 2 cut(s) 461, 1432
TspDTI ATGAA 7 cut(s) 129, 150, 339, 393, 430, 484, 967
TspGWI ACGGA 2 cut(s) 73, 1404
VpaK11BI GGWCC 1 cut(s) 1293
XagI CCTNNNNNAGG 1 cut(s) 638
XapI RAATTY 3 cut(s) 54, 864, 1310
XbaI TCTAGA 1 cut(s) 893
XceI RCATGY 4 cut(s) 9, 730, 874, 1234
XmaJI CCTAGG 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 2 cut(s) 626, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.