Rh7AG016000

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014745
Physical Location & Seq
Reverse (-)
1058793 .. 1061202
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG016000.1

Sequence Viewer

Length: 1422 bp
ATGGCTTGTTCATCATCAAATATATGGAGGGTGACAACACTTGACTCTGGCTCAAAAATTTCAAGCTACTTTAGCAACCCTGCCTCCATGTTCCTCTTCTTTTCCACTACCATTCCCAACAAACGGCGGCGTCTAATCGCTTGTTCATTAGATCCCATCTCCACTTCATCACTAACATCTCAGAATGCTACTCACAAGAACAGAACAGACAGGAAATCTGTTGAAATTCAAAGAACCAATGATGGGACTTTCTCTGTTTTACACAGTGATGAGCACAAACCATATGAGAAGAAGATCAAGAACAGCAAGAAGGATGAAGCTAATAAGGGGTTGAAATCTGGTTCCAAAAAGCGACGACCTTTGTGGCAAAAGGCTTTCTTTGCATCCAAGAAGATGAGGAGTATTTTGTTGCTCAATGCAATCACAATTGTCTATGCAAGTGACATTCCAGTTATTAAAGAAGTTGAAACTATCATGGACCCAGCAGCCTTCTCAGCGGTGCGGTTTATCATGTCAGCAATTCCATTTCTGCCATTCGTGTTTCGATCACCGGGTGATGTTCAGACCCGAAATGCAGGGATTGAGTTGGGGTTCTGGATCAGTCTAGGCTACCTTCTTGAGGCACTCGGTTTACTTACATCTGATGCTGGCCGTGCATCGTTTATTTCCCTCTTCACAGTTATTGTGGTACCTTTGCTTGATGGCTTGTTTGGATCAATCATACCTGCTAGATCCTGGTTCGGAGTTCTCATGTCTGCTCTTGGGGTTGGTATGCTGGAATGTAGTGGATCACCTCCAAATGTTGGAGATCTCTTCAACTTCTTGAGTGCAATATTTTTCGGAATTCACATGCTTCGAACTGAGCATATTTCAAGAAGCACAAAGAAGGAGAACTTCCTAGCAATTCTTGGATATGAGGTCAGTGTTGTTGCTGTCTTATCCACAATCTGGGTTTTTATTGGAGGGTGGTTTGATGGTGCCCAAAGTTCCAACCAATTAACATGGACGTGGACAGAGATGTGGGATTGGCTTGTTGCATTTCCATGGATACCTGCACTTTATACTGGTGCATTTTCAACTGGGATATGCTTATGGATTGAGATTGCAGCGATGCGCGATGTTTCAGCAACAGAAACAGCCATAATTTATGGGCTGGAGCCACTGTGGGGAGCTGGATTTGCATGGTTTCTTCTTGGTGAAAGATGGGGAGCACTTGGATGGATCGGTGCAGCTCTTGTGCTTGGTGGAAGCCTAGTGGTGCAGATATTTGGGTCCTCAACTTCAAAAGAATTTATAGTTTCTGAAGAGAGCAAGGAGAAAGGTGATCTCCTAAGGGTTCCAGTTGACCAAAAATTACAAAATAGTCTCTCTACTTCTCCATTAGTTGTCAGGTCCAAAAAGGATCTAACAGATTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

473

Amino Acids

52.26

Weight (kDa)

9.11

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 134 - 258 6.3e-09 EamA-like transporter family
EamA PF00892 269 - 420 1.4e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17290 FvH4_5g17290 FvH4_5g17290
malus_domestica MD06G1226600.v1.1
prunus_persica Prupe.5G232800_v2.0.a1 Prupe.5G232900_v2.0.a1
pyrus_communis pycom06g20290
rosa_chinensis RchiOBHm_Chr7g0178481
rosa_laevigata RLG00000005443
rosa_multiflora Rmu_sc0010235.1_g000003
rosa_roxburghii Rroxscaffold_3G00274860
rosa_rugosa Rorug06G0417500
rosa_samantha Rh7AG016000 Rh7BG015600 Rh7CG016300 Rh7DG016000
rosa_wichuraiana Rw7G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 733, 1060
Acc65I GGTACC 1 cut(s) 688
AccB1I GGYRCC 2 cut(s) 688, 977
AccB7I CCANNNNNTGG 2 cut(s) 803, 948
AccII CGCG 1 cut(s) 1116
AciI CCGC 3 cut(s) 127, 497, 502
AclWI GGATC 7 cut(s) 146, 605, 721, 726, 796, 1229, 1410
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 4 cut(s) 57, 225, 843, 1289
AcuI CTGAAG 1 cut(s) 1323
AcyI GRCGYC 1 cut(s) 130
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 1 cut(s) 690
AfiI CCNNNNNNNGG 6 cut(s) 123, 243, 619, 803, 948, 1166
AgsI TTSAA 9 cut(s) 63, 224, 230, 334, 467, 817, 873, 1077, 1284
AjiI CACGTC 1 cut(s) 1008
AjnI CCWGG 1 cut(s) 734
AluBI AGCT 4 cut(s) 66, 320, 1172, 1232
AluI AGCT 4 cut(s) 66, 320, 1172, 1232
Alw21I GWGCWC 2 cut(s) 276, 1213
Alw26I GTCTC 1 cut(s) 1370
AlwI GGATC 7 cut(s) 146, 605, 721, 726, 796, 1229, 1410
AoxI GGCC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 485, 1106, 1229
ApoI RAATTY 4 cut(s) 57, 225, 843, 1289
Asp718I GGTACC 1 cut(s) 688
AspLEI GCGC 1 cut(s) 1116
AspS9I GGNCC 3 cut(s) 478, 1272, 1392
AsuC2I CCSGG 1 cut(s) 552
AsuHPI GGTGA 6 cut(s) 43, 540, 566, 783, 1208, 1334
AsuII TTCGAA 1 cut(s) 856
AvaII GGWCC 3 cut(s) 478, 1272, 1392
AxyI CCTNAGG 1 cut(s) 1331
BaeGI GKGCMC 1 cut(s) 982
BanI GGYRCC 2 cut(s) 688, 977
Bbv12I GWGCWC 2 cut(s) 276, 1213
BbvI GCAGC 3 cut(s) 497, 1118, 1241
BccI CCATC 6 cut(s) 164, 236, 695, 968, 1197, 1212
BceAI ACGGC 2 cut(s) 140, 636
BciT130I CCWGG 1 cut(s) 736
BciVI GTATCC 1 cut(s) 1041
BcnI CCSGG 1 cut(s) 552
BcoDI GTCTC 1 cut(s) 1370
BfaI CTAG 4 cut(s) 605, 729, 899, 1253
BfmI CTRYAG 1 cut(s) 1418
BfuAI ACCTGC 2 cut(s) 733, 1060
BfuI GTATCC 1 cut(s) 1041
BglII AGATCT 1 cut(s) 808
BisI GCNGC 4 cut(s) 128, 486, 1107, 1230
BlsI GCNGC 4 cut(s) 129, 487, 1108, 1231
Bme1390I CCNGG 2 cut(s) 552, 736
Bme18I GGWCC 3 cut(s) 478, 1272, 1392
BmgBI CACGTC 1 cut(s) 1008
BmgT120I GGNCC 3 cut(s) 478, 1272, 1392
BmiI GGNNCC 7 cut(s) 343, 480, 690, 979, 1158, 1273, 1338
BmrFI CCNGG 2 cut(s) 552, 736
BmrI ACTGGG 1 cut(s) 1089
BmsI GCATC 4 cut(s) 392, 634, 665, 1101
BmuI ACTGGG 1 cut(s) 1089
BpmI CTGGAG 1 cut(s) 1175
Bpu14I TTCGAA 1 cut(s) 856
BpuEI CTTGAG 2 cut(s) 638, 844
BpuMI CCSGG 1 cut(s) 552
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 1043
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1148, 1178
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 243, 619, 803, 948, 1166
Bse1I ACTGG 4 cut(s) 449, 1069, 1084, 1340
Bse21I CCTNAGG 1 cut(s) 1331
BseBI CCWGG 1 cut(s) 736
BseDI CCNNGG 1 cut(s) 1043
BseGI GGATG 3 cut(s) 319, 383, 1223
BseLI CCNNNNNNNGG 6 cut(s) 123, 243, 619, 803, 948, 1166
BseMII CTCAG 3 cut(s) 194, 507, 852
BseNI ACTGG 4 cut(s) 449, 1069, 1084, 1340
BseRI GAGGAG 1 cut(s) 412
BseSI GKGCMC 1 cut(s) 982
BseXI GCAGC 3 cut(s) 497, 1118, 1241
BseYI CCCAGC 1 cut(s) 481
BsgI GTGCAG 3 cut(s) 1038, 1248, 1280
Bsh1236I CGCG 1 cut(s) 1116
BshFI GGCC 1 cut(s) 651
BshNI GGYRCC 2 cut(s) 688, 977
BsiHKAI GWGCWC 2 cut(s) 276, 1213
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 6 cut(s) 123, 243, 619, 803, 948, 1166
BsmAI GTCTC 1 cut(s) 1370
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 1 cut(s) 651
Bsp119I TTCGAA 1 cut(s) 856
Bsp1286I GDGCHC 3 cut(s) 276, 982, 1213
Bsp19I CCATGG 1 cut(s) 1043
BspACI CCGC 3 cut(s) 127, 497, 502
BspANI GGCC 1 cut(s) 651
BspCNI CTCAG 3 cut(s) 193, 506, 853
BspFNI CGCG 1 cut(s) 1116
BspLI GGNNCC 7 cut(s) 343, 480, 690, 979, 1158, 1273, 1338
BspMI ACCTGC 2 cut(s) 733, 1060
BspPI GGATC 7 cut(s) 146, 605, 721, 726, 796, 1229, 1410
BspT104I TTCGAA 1 cut(s) 856
BspT107I GGYRCC 2 cut(s) 688, 977
BsrI ACTGG 4 cut(s) 449, 1069, 1084, 1340
BssECI CCNNGG 1 cut(s) 1043
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 1043
Bst2UI CCWGG 1 cut(s) 736
Bst4CI ACNGT 3 cut(s) 266, 679, 1164
Bst6I CTCTTC 4 cut(s) 101, 677, 818, 1299
BstACI GRCGYC 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 856
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 4 cut(s) 180, 493, 861, 1331
BstDSI CCRYGG 1 cut(s) 1043
BstENI CCTNNNNNAGG 1 cut(s) 617
BstF5I GGATG 3 cut(s) 319, 383, 1223
BstFNI CGCG 1 cut(s) 1116
BstHHI GCGC 1 cut(s) 1116
BstMAI GTCTC 1 cut(s) 1370
BstMWI GCNNNNNNNGC 5 cut(s) 72, 380, 494, 653, 1178
BstNI CCWGG 1 cut(s) 736
BstNSI RCATGY 1 cut(s) 853
BstSCI CCNGG 2 cut(s) 550, 734
BstSFI CTRYAG 1 cut(s) 1418
BstSLI GKGCMC 1 cut(s) 982
BstUI CGCG 1 cut(s) 1116
BstV1I GCAGC 3 cut(s) 497, 1118, 1241
BstX2I RGATCY 4 cut(s) 151, 731, 808, 1402
BstYI RGATCY 4 cut(s) 151, 731, 808, 1402
Bsu36I CCTNAGG 1 cut(s) 1331
BsuI GTATCC 1 cut(s) 1041
BsuRI GGCC 1 cut(s) 651
BtgI CCRYGG 1 cut(s) 1043
BtgZI GCGATG 2 cut(s) 1124, 1131
BtrI CACGTC 1 cut(s) 1008
BtsCI GGATG 3 cut(s) 319, 383, 1223
BtsIMutI CAGTG 3 cut(s) 271, 928, 1160
BveI ACCTGC 2 cut(s) 733, 1060
Cac8I GCNNGC 1 cut(s) 649
CfoI GCGC 1 cut(s) 1116
Cfr13I GGNCC 3 cut(s) 478, 1272, 1392
CseI GACGC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 689
CviAII CATG 8 cut(s) 88, 475, 511, 751, 850, 1002, 1044, 1182
CviQI GTAC 1 cut(s) 689
DdeI CTNAG 4 cut(s) 180, 493, 861, 1331
DraIII CACNNNGTG 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 649
Eam1104I CTCTTC 4 cut(s) 101, 677, 818, 1299
EarI CTCTTC 4 cut(s) 101, 677, 818, 1299
Eco130I CCWWGG 1 cut(s) 1043
Eco47I GGWCC 3 cut(s) 478, 1272, 1392
Eco57I CTGAAG 1 cut(s) 1323
Eco81I CCTNAGG 1 cut(s) 1331
EcoNI CCTNNNNNAGG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 1272
EcoRI GAATTC 1 cut(s) 843
EcoRII CCWGG 1 cut(s) 734
EcoT14I CCWWGG 1 cut(s) 1043
ErhI CCWWGG 1 cut(s) 1043
FaeI CATG 8 cut(s) 91, 478, 514, 754, 853, 1005, 1047, 1185
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 878, 910
FaqI GGGAC 1 cut(s) 259
FatI CATG 8 cut(s) 87, 474, 510, 750, 849, 1001, 1043, 1181
FauNDI CATATG 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 128, 486, 1107, 1230
FokI GGATG 3 cut(s) 326, 370, 1230
Fsp4HI GCNGC 4 cut(s) 128, 486, 1107, 1230
FspBI CTAG 4 cut(s) 605, 729, 899, 1253
GlaI GCGC 1 cut(s) 1115
GluI GCNGC 4 cut(s) 128, 486, 1107, 1230
GsaI CCCAGC 1 cut(s) 485
GsuI CTGGAG 1 cut(s) 1175
HaeIII GGCC 1 cut(s) 651
HapII CCGG 1 cut(s) 551
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 1116
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 8 cut(s) 91, 478, 514, 754, 853, 1005, 1047, 1185
Hin6I GCGC 1 cut(s) 1114
HinP1I GCGC 1 cut(s) 1114
HincII GTYRAC 1 cut(s) 1345
HindII GTYRAC 1 cut(s) 1345
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 551
HphI GGTGA 6 cut(s) 43, 540, 566, 783, 1208, 1334
Hpy166II GTNNAC 3 cut(s) 632, 1011, 1345
Hpy188I TCNGA 6 cut(s) 183, 564, 643, 743, 842, 1303
Hpy188III TCNNGA 5 cut(s) 298, 595, 617, 823, 873
Hpy8I GTNNAC 3 cut(s) 632, 1011, 1345
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 4 cut(s) 304, 499, 623, 880
HpyCH4III ACNGT 3 cut(s) 266, 679, 1164
HpyCH4IV ACGT 1 cut(s) 1007
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 380, 494, 653, 1178
HpyF3I CTNAG 4 cut(s) 180, 493, 861, 1331
HpySE526I ACGT 1 cut(s) 1007
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 8 cut(s) 91, 478, 514, 754, 853, 1005, 1047, 1185
HspAI GCGC 1 cut(s) 1114
KpnI GGTACC 1 cut(s) 692
LmnI GCTCC 3 cut(s) 1156, 1169, 1208
Lsp1109I GCAGC 3 cut(s) 497, 1118, 1241
LweI GCATC 4 cut(s) 392, 634, 665, 1101
MaeI CTAG 4 cut(s) 605, 729, 899, 1253
MaeII ACGT 1 cut(s) 1007
MaeIII GTNAC 2 cut(s) 31, 440
MboII GAAGA 8 cut(s) 88, 301, 304, 403, 664, 805, 1181, 1316
MfeI CAATTG 1 cut(s) 426
MflI RGATCY 4 cut(s) 151, 731, 808, 1402
MhlI GDGCHC 3 cut(s) 276, 982, 1213
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 784, 1014
MseI TTAA 2 cut(s) 456, 998
MslI CAYNNNNRTG 4 cut(s) 267, 1006, 1042, 1216
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 1 cut(s) 551
MspR9I CCNGG 2 cut(s) 552, 736
MunI CAATTG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 736
MvnI CGCG 1 cut(s) 1116
MwoI GCNNNNNNNGC 5 cut(s) 72, 380, 494, 653, 1178
NciI CCSGG 1 cut(s) 552
NcoI CCATGG 1 cut(s) 1043
NdeI CATATG 1 cut(s) 283
NlaIII CATG 8 cut(s) 91, 478, 514, 754, 853, 1005, 1047, 1185
NlaIV GGNNCC 7 cut(s) 343, 480, 690, 979, 1158, 1273, 1338
NmuCI GTSAC 2 cut(s) 31, 440
NspI RCATGY 1 cut(s) 853
NspV TTCGAA 1 cut(s) 856
PctI GAATGC 1 cut(s) 190
PflMI CCANNNNNTGG 2 cut(s) 803, 948
PkrI GCNGC 4 cut(s) 129, 487, 1108, 1231
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PpuMI RGGWCCY 1 cut(s) 1272
Psp5II RGGWCCY 1 cut(s) 1272
Psp6I CCWGG 1 cut(s) 734
PspFI CCCAGC 1 cut(s) 481
PspGI CCWGG 1 cut(s) 734
PspN4I GGNNCC 7 cut(s) 343, 480, 690, 979, 1158, 1273, 1338
PspPI GGNCC 3 cut(s) 478, 1272, 1392
PspPPI RGGWCCY 1 cut(s) 1272
PsuI RGATCY 4 cut(s) 151, 731, 808, 1402
RsaI GTAC 1 cut(s) 690
RsaNI GTAC 1 cut(s) 689
RseI CAYNNNNRTG 4 cut(s) 267, 1006, 1042, 1216
SaqAI TTAA 2 cut(s) 456, 998
SatI GCNGC 4 cut(s) 128, 486, 1107, 1230
Sau96I GGNCC 3 cut(s) 478, 1272, 1392
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 2 cut(s) 552, 736
SduI GDGCHC 3 cut(s) 276, 982, 1213
SfaNI GCATC 4 cut(s) 392, 634, 665, 1101
SfcI CTRYAG 1 cut(s) 1418
SfuI TTCGAA 1 cut(s) 856
SinI GGWCC 3 cut(s) 478, 1272, 1392
SmiMI CAYNNNNRTG 4 cut(s) 267, 1006, 1042, 1216
SmlI CTYRAG 2 cut(s) 617, 823
SmoI CTYRAG 2 cut(s) 617, 823
SsiI CCGC 3 cut(s) 127, 497, 502
SspI AATATT 1 cut(s) 834
SspMI CTAG 4 cut(s) 605, 729, 899, 1253
StyD4I CCNGG 2 cut(s) 550, 734
StyI CCWWGG 1 cut(s) 1043
TaaI ACNGT 3 cut(s) 266, 679, 1164
TaiI ACGT 1 cut(s) 1010
TaqI TCGA 2 cut(s) 544, 856
TauI GCSGC 1 cut(s) 130
Tru1I TTAA 2 cut(s) 456, 998
Tru9I TTAA 2 cut(s) 456, 998
TscAI CASTG 3 cut(s) 271, 928, 1167
TseFI GTSAC 2 cut(s) 31, 440
TseI GCWGC 3 cut(s) 485, 1106, 1229
Tsp45I GTSAC 2 cut(s) 31, 440
TspDTI ATGAA 3 cut(s) 135, 156, 330
TspRI CASTG 3 cut(s) 271, 928, 1167
Van91I CCANNNNNTGG 2 cut(s) 803, 948
VpaK11BI GGWCC 3 cut(s) 478, 1272, 1392
XagI CCTNNNNNAGG 1 cut(s) 617
XapI RAATTY 4 cut(s) 57, 225, 843, 1289
XceI RCATGY 1 cut(s) 853
XspI CTAG 4 cut(s) 605, 729, 899, 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.